Plant extract rich in functional flavone compounds and preparation method and application thereof

Abstract

The invention discloses a preparation method of a plant extract rich in functional flavone compounds, which comprises the following steps: removing impurities from Kushui rose calyx, airing in a shady, cool and ventilated place, and crushing for later use; the preparation method comprises the following steps: weighing Kushui rose calyx powder, extracting twice under an ultrasonic condition by taking an ethanol solution as an extraction solvent, and merging supernate to obtain an extracting solution rich in flavonoid substances. The invention further provides the plant extract rich in the functional flavone compounds and application of the plant extract rich in the functional flavone compounds, according to the plant extract rich in the functional flavone compounds and the preparation method and application of the plant extract rich in the functional flavone compounds, the method is easy and convenient to operate, the extraction efficiency is high, the cost is low, and the plant extract rich in the functional flavone compounds and the application of the plant extract rich in the functional flavone compounds are obtained. And resource utilization of Kushui rose processing by-products can be realized.

Description

Technical Field

[0001] This invention belongs to the technical field of natural product extraction methods, specifically relating to plant extracts rich in functional flavonoid compounds, as well as the preparation methods and applications of the aforementioned plant extracts. Background Technology

[0002] The bitter rose (Rosa sertata × Rosa rugosa) possesses a rich aroma and high medicinal and edible value. Bitter rose flowers are rich in polyphenols, flavonoids, and other chemical components, exhibiting various biological activities such as antioxidant, anti-inflammatory, antibacterial, and antithrombotic effects. These functional flavonoids have broad application prospects in the food, cosmetics, and pharmaceutical industries.

[0003] Currently, research on the extraction of rose flavonoids mainly focuses on petals or buds, with less utilization of the calyx. In the extraction of rose essential oil and the production of fresh flower cakes, the calyx is often discarded in large quantities as a byproduct, causing resource waste and environmental pollution. Existing technologies, such as Chinese Patent CN121490006A (publication date: 2026.02.10) and Chinese Patent CN106317005B (publication date: 2020.331), disclose methods for extracting flavonoids from dried rose buds and rose petal residue water, respectively, but neither involves the extraction and utilization of specific functional flavonoid components (catechins, dihydromyricetin, quercetin) from the calyx of the bitter rose. There are currently no research reports on the extraction of these functional flavonoid compounds using the calyx of the bitter rose as raw material; their composition and content are still unclear, and systematic extraction process research is lacking. Therefore, developing an extraction method for the calyx of the bitter rose that is simple to operate, has high extraction efficiency, and can enrich flavonoid functional components such as catechin, dihydromyricetin, and quercetin is of great practical significance and economic value. This method would fill the research gap in this field and realize the high-value utilization of rose processing by-products. Summary of the Invention

[0004] The first objective of this invention is to provide a method for preparing plant extracts rich in functional flavonoids. This method is simple to operate, has high extraction efficiency, and is low in cost, enabling the resource utilization of by-products from the processing of bitter rose.

[0005] A second objective of this invention is to provide a plant extract rich in functional flavonoid compounds obtained by the above method.

[0006] A third objective of this invention is to provide applications of the aforementioned plant extracts.

[0007] The first technical solution adopted in this invention is: a method for preparing plant extracts rich in functional flavonoids, comprising the following steps: removing impurities from the calyx of the bitter rose, drying it in a cool and ventilated place, and pulverizing it for later use; weighing the powder of the calyx of the bitter rose, using ethanol solution as the extraction solvent, extracting it under ultrasonic conditions, extracting it twice, and combining the supernatants to obtain an extract rich in flavonoids.

[0008] The first technical solution adopted in this invention is further characterized by: Furthermore, the preparation method of plant extracts rich in functional flavonoids is specifically carried out according to the following steps: Step 1: Remove impurities from the calyxes of the bitter rose, dry them in a cool, ventilated place, and then crush them for later use; Step 2: Weigh out the calyx powder of bitter rose and extract it using ethanol solution as the extraction solvent. Extract the powder under the following conditions: ethanol solution concentration of 40%-50%, material-liquid ratio of 1:20-1:40 g / mL, ultrasonic temperature of 40-55℃, and ultrasonic time of 20-40 min. Step 3: Extract twice following the above steps, combine the supernatants to obtain an extract rich in flavonoids.

[0009] Furthermore, in step 1, a high-speed universal pulverizer is used to pulverize the dried calyx into fine powder with a relatively uniform particle size of 60-80 mesh.

[0010] The second technical solution adopted in this invention is: a plant extract rich in functional flavonoid compounds prepared according to the above method.

[0011] The second technical solution adopted in this invention is further characterized by: Furthermore, the aforementioned plant extracts are rich in flavonoid glycosides and aglycones, including catechins, dihydromyricetin, and quercetin.

[0012] The third technical solution adopted in this invention is: the application of the above-mentioned plant extracts rich in functional flavonoids in the preparation of antioxidant, anti-inflammatory, and metabolic regulating drugs.

[0013] The fourth technical solution adopted in this invention is: the application of the above-mentioned plant extracts rich in functional flavonoids in the preparation of antioxidant, anti-inflammatory, anti-tumor, and hypoglycemic drugs.

[0014] The fifth technical solution adopted in this invention is: the application of the above-mentioned plant extracts rich in functional flavonoids in the preparation of antioxidant, anti-inflammatory, and hypoglycemic drugs.

[0015] The beneficial effects of this invention are: 1. The method of this invention is the first to develop the preparation of functional plant extracts from calyx resources: For the first time, functional plant extracts rich in catechins, dihydromyricetin and quercetin are prepared using the calyx of the bitter rose as raw material, filling the research gap in this field and realizing the high-value utilization of rose processing by-products, which is of pioneering significance.

[0016] 2. The method of the present invention has high extraction efficiency: By optimizing the extraction parameters (ethanol solution concentration 42%, material-liquid ratio 1:32, ultrasonic temperature 50℃, ultrasonic time 32 min), flavonoids are fully dissolved, and the total flavonoid yield can reach 9.60%.

[0017] 3. The plant extract of the present invention has a high content of functional components: The plant extract of the calyx of the bitter rose prepared by the present invention is rich in three main functional flavonoids, namely catechin (0.72 mg / mL), dihydromyricetin (0.34 mg / mL), and quercetin (0.31 mg / mL). It can be used as a high-quality functional raw material in food, cosmetics, medicine and other fields, and has high biological activity and application value.

[0018] 4. The method of the present invention is simple to operate: it adopts ultrasonic-assisted extraction, the equipment is simple, the operation is convenient, the extraction time is short, and it is suitable for industrial production.

[0019] 5. The method of the present invention has significant economic benefits: the method is simple and low-cost, and uses the calyx, a by-product of rose processing, as raw material to prepare high-value-added functional plant extracts, realizing the transformation of waste into treasure. It provides a new way for the development of the entire industrial chain of bitter rose and the creation of functional products, and has high economic value and market application prospects. Attached Figure Description

[0020] Figure 1 The effect of different ethanol solution concentrations on the yield of total flavonoids; Figure 2 The effect of different material-to-liquid ratios on the yield of total flavonoids; Figure 3 The effect of different ultrasonic temperatures on the yield of total flavonoids; Figure 4 The effect of different ultrasound times on the yield of total flavonoids; Figure 5 This is the mass spectrum of catechins; Figure 6 The mass spectrum of dihydromyricetin; Figure 7 This is the mass spectrum of quercetin. Detailed Implementation

[0021] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0022] This invention provides a method for preparing plant extracts rich in functional flavonoid components such as catechin, dihydromyricetin, and quercetin, comprising the following steps: Step 1: Remove impurities from the calyxes of the bitter rose, dry them in a cool, ventilated place, and then crush them for later use; Preferably, a high-speed universal pulverizer is used to pulverize the dried calyx into fine powder with a relatively uniform particle size of 60-80 mesh. Step 2: Weigh out the calyx powder of bitter rose and extract it using ethanol solution as the extraction solvent. Extract the powder under the following conditions: ethanol solution concentration of 40%-50%, material-to-liquid ratio of 1:20-40 g / mL, ultrasonic temperature of 40-55℃, and ultrasonic time of 20-40 min. Preferably, the calyx powder of bitter rose is accurately weighed, and extraction is carried out using ethanol solution as the extraction solvent under the conditions of ethanol solution concentration of 42%, material-to-liquid ratio of 1:32, ultrasonic temperature of 50℃, and ultrasonic time of 32min. Step 3: Extract twice using the above method, combine the supernatants to obtain an extract rich in flavonoids, and then test the extract.

[0023] In step 1, a high-speed universal pulverizer is used to pulverize the dried calyx into fine powder with relatively uniform particle size. Compared with manual crushing or other coarse pulverizing methods, the uniform powder particle size can ensure that each particle has a similar contact area with the extraction solvent, so that the flavonoids in the cells can be extracted more fully and evenly, thereby improving the extraction efficiency. At the same time, since the cell structure inside the dried calyx is intact, the flavonoids are wrapped inside the cells, and the solvent can penetrate into the cells more easily after pulverization, thereby increasing the yield of flavonoids.

[0024] In step 2, the extraction solvent was an ethanol solution. A 42% (v / v) ethanol solution was chosen as the solvent after considering the polarity and solubility characteristics of the three flavonoids. Catechins (flavanols) have multiple ortho- and olfactory hydroxyl groups, a high proportion of hydrophilic hydroxyl groups, relatively high molecular polarity, and good water solubility, being soluble in hot water and low-concentration ethanol. Dihydromyricetin (dihydroflavonols) has a hydrogen-saturated myricetin nucleus, numerous phenolic hydroxyl groups (multi-hydroxyl substitution), abundant hydrophilic groups, and moderate to strong polarity. Its solubility in pure water is poor, it is readily soluble in low-concentration alcohols, and its solubility in high-concentration ethanol is also poor. Quercetin (flavonoid glycosides) has a structure characterized by the introduction of hydrophilic sugar groups after glycosylation, improving water solubility, but still retaining the hydrophobic aglycone skeleton. Its solubility in pure water is poor, and low to medium concentration ethanol is the best fit. 42% ethanol has moderate polarity, which can effectively dissolve flavonoids while reducing the dissolution of some hydrophilic impurities and non-flavonoid lipid-soluble impurities.

[0025] In step 2, the material-to-liquid ratio is 1:32. This ratio ensures that there is enough solvent to fully wet and dissolve the flavonoids in the calyx powder, providing a sufficient solvent environment for the target components to diffuse and dissolve. If the material-to-liquid ratio is too small, such as 1:10, some flavonoids will not dissolve sufficiently, thus reducing the yield. If the material-to-liquid ratio is too large, such as 1:50, it will increase the energy consumption and cost of the subsequent concentration process, and the yield improvement will not be significant.

[0026] The ultrasonic temperature in step 2 is 50℃. This temperature is an optimized choice. Too high a temperature (such as above 70℃) may damage the structural stability of flavonoids and reduce their biological activity; too low a temperature (such as below 30℃) will slow down the thermal motion of molecules, reduce the diffusion rate of flavonoids, and decrease the extraction efficiency. At 50℃, the thermal motion of molecules is intensified, which can accelerate the dissolution of flavonoids from the calyx powder while ensuring that their structural integrity is not damaged.

[0027] The extraction in step 2 is ultrasound-assisted extraction, with an ultrasound time of 32 minutes. Utilizing the cavitation, mechanical, and thermal effects of ultrasound, the plant cell walls of the calyx of the bitter rose can be effectively destroyed, accelerating the release, diffusion, and dissolution of flavonoids within the cells. The 32-minute extraction time is optimized to ensure that the flavonoids are fully dissolved in the solvent to achieve extraction equilibrium, without increasing unnecessary energy consumption due to excessive time. If the time is too short, the extraction will be insufficient; if the time is too long, it may lead to increased dissolution of impurities or degradation of flavonoids.

[0028] Step 3, which involves two extractions, refers to performing three independent ultrasonic extractions on the same batch of calyx powder. The supernatants from the first two extractions are combined, and the extract obtained from the third extraction is used as the solvent for the first extraction in the next round. However, only the supernatant from the first two extractions is used for calculation. This extraction method can further dissolve the flavonoids remaining in the calyx powder after the first extraction, maximizing the yield of total flavonoids, ensuring full utilization of raw materials, saving ethanol resources, and recycling them. A single extraction often fails to completely dissolve the target components, while two extractions can significantly improve the yield.

[0029] The supernatant in step 3 is the extract obtained by combining two extractions. After two extractions, the solution will contain extracted flavonoids, undissolved impurities, and solvent. Centrifugation is used to separate the extracted flavonoids from most of the solid impurities. This extract is rich in three main flavonoids: catechin, dihydromyricetin, and quercetin. Catechins have various biological activities such as antioxidant, anti-inflammatory, and metabolic regulation; dihydromyricetin has antioxidant, anti-inflammatory, anti-tumor, and hypoglycemic effects; and quercetin has antioxidant, anti-inflammatory, anti-tumor, and cardiovascular protective effects.

[0030] This invention is the first to utilize the calyx of the bitter rose (Rosa kusnezoffii) for the preparation of plant extracts rich in catechins, dihydromyricetin, and quercetin. Unlike existing technologies that primarily focus on rose buds or petals, this invention innovatively uses the calyx, a processing byproduct, as a raw material. This solves the resource waste and environmental pollution problems caused by discarding calyxes during rose essential oil extraction and fresh flower cake processing, while also enabling the preparation of plant extracts rich in various functional flavonoid components. The bitter rose calyx plant extract obtained by this method achieves a total flavonoid yield of 6.72%-9.60%, and is rich in the three main functional flavonoids: catechin (0.42-0.72 mg / mL), dihydromyricetin (0.23-0.34 mg / mL), and quercetin (0.17-0.31 mg / mL). This invention not only fills the gap in the research of functional plant extracts from the calyx of the bitter rose, turning waste into treasure, but also has the advantages of simple process, low cost and environmental protection, providing new ideas for the development of the entire bitter rose industry chain and the creation of high value-added functional products.

[0031] The technical solution of the present invention will be further described below through embodiments.

[0032] Example 1 Step 1: Raw material processing: Remove impurities from the calyxes of the bitter rose, dry them in a cool, ventilated place, and then pulverize them with a high-speed universal pulverizer to obtain bitter rose calyx powder, which is then stored for later use.

[0033] Step 2, Extraction Method: Accurately weigh 5 portions of *Rosa kusnezoffii* calyx powder and place them separately in Erlenmeyer flasks. Add ethanol solutions with volume concentrations of 30%, 40%, 50%, 60%, and 70% respectively at a material-to-liquid ratio of 1:20. Extract by ultrasonication at 50℃ for 30 min, then centrifuge at 8000 r / min for 10 min and collect the supernatant. Perform a second extraction on the precipitate residue under the same conditions, and combine the supernatants from both extractions to obtain the flavonoid extract from *Rosa kusnezoffii* calyx.

[0034] Step 3, Content Determination: After determination, if... Figure 1 As shown, under different ethanol solution concentrations, the yield of flavonoids from the calyx of *Rosa broccoli* ranged from 6.72% to 8.18%, the content of catechins ranged from 0.42 to 0.61 mg / mL, the content of dihydromyricetin ranged from 0.20 to 0.29 mg / mL, and the content of quercetin ranged from 0.18 to 0.26 mg / mL.

[0035] Example 2 Step 1: Raw material processing: Remove impurities from the calyxes of the bitter rose, dry them in a cool, ventilated place, and then pulverize them with a high-speed universal pulverizer to obtain bitter rose calyx powder, which is then stored for later use.

[0036] Step 2, Extraction Method: Accurately weigh 5 portions of *Rosa kusnezoffii* calyx powder and place them separately in Erlenmeyer flasks. Add 60% ethanol solution at material-to-liquid ratios of 1:10, 1:20, 1:30, 1:40, and 1:50 respectively. Extract by ultrasonication at 50℃ for 30 min, then centrifuge at 8000 r / min for 10 min and collect the supernatant. Perform a second extraction on the precipitate residue under the same conditions, and combine the supernatants from both extractions to obtain the flavonoid extract from *Rosa kusnezoffii* calyx.

[0037] Step 3, Content Determination: After determination, if... Figure 2 As shown, under different material-to-liquid ratios, the yield of flavonoids from the calyx of *Rosa chinensis* ranged from 7.49% to 9.59%, the content of catechins ranged from 0.49 to 0.71 mg / mL, the content of dihydromyricetin ranged from 0.23 to 0.34 mg / mL, and the content of quercetin ranged from 0.17 to 0.30 mg / mL.

[0038] Example 3 Step 1: Raw material processing: Remove impurities from the calyxes of the bitter rose, dry them in a cool, ventilated place, and then pulverize them with a high-speed universal pulverizer to obtain bitter rose calyx powder, which is then stored for later use.

[0039] Step 2, Extraction Method: Accurately weigh 5 portions of *Rosa kusnezoffii* calyx powder and place them separately in Erlenmeyer flasks. Add 60% ethanol solution at a solid-liquid ratio of 1:30. Extract using ultrasound at 30℃, 40℃, 50℃, 60℃, and 70℃ for 30 min each time. Then centrifuge at 8000 r / min for 10 min and collect the supernatant. Perform a second extraction on the precipitate residue under the same conditions, and combine the supernatants from both extractions to obtain the flavonoid extract from *Rosa kusnezoffii* calyx.

[0040] Step 3, Content Determination: After determination, if... Figure 3 As shown, under different ultrasonic temperature conditions, the yield of flavonoids in the calyx of *Rosa kusnezoffii* ranged from 6.79% to 7.60%, the content of catechins ranged from 0.45 to 0.54 mg / mL, the content of dihydromyricetin ranged from 0.23 to 0.27 mg / mL, and the content of quercetin ranged from 0.25 to 0.32 mg / mL.

[0041] Example 4 Step 1: Raw material processing: Remove impurities from the calyxes of the bitter rose, dry them in a cool, ventilated place, and then pulverize them with a high-speed universal pulverizer to obtain bitter rose calyx powder, which is then stored for later use.

[0042] Step 2, Extraction Method: Accurately weigh 5 portions of *Rosa kusnezoffii* calyx powder and place them separately in Erlenmeyer flasks. Add 60% ethanol solution at a solid-liquid ratio of 1:30. Extract by ultrasonication at 50℃ for 20 min, 30 min, 40 min, 50 min, and 60 min respectively. Then centrifuge at 8000 r / min for 10 min and collect the supernatant. Perform a second extraction on the precipitate residue under the same conditions, and combine the supernatants from both extractions to obtain the flavonoid extract from *Rosa kusnezoffii* calyx.

[0043] Step 3, Content Determination: After determination, if... Figure 4 As shown, under different ultrasound time conditions, the yield of flavonoids in the calyx of *Rosa kusnezoffii* ranged from 7.39% to 7.73%, the content of catechins ranged from 0.54 to 0.59 mg / mL, the content of dihydromyricetin ranged from 0.28 to 0.31 mg / mL, and the content of quercetin ranged from 0.20 to 0.22 mg / mL.

[0044] Example 5 The plant extract rich in functional flavonoids was prepared according to the method described above.

[0045] The plant extract contains abundant flavonoid glycosides and aglycones, including catechins, dihydromyricetin, and quercetin.

[0046] Example 6 The above-mentioned plant extracts rich in functional flavonoids are used in the preparation of antioxidant, anti-inflammatory, and metabolic regulating drugs.

[0047] This embodiment uses molecular docking technology to evaluate the binding ability of catechins, the main active ingredient in flavonoid extracts, to various key functional proteins, in order to reveal their potential molecular mechanisms in antioxidation, anti-inflammation, metabolic regulation, and anti-tumor effects. Two antioxidant-related proteins (SOD1, KEAP1), two inflammation-related proteins (IL6, TNF), two tumor-related proteins (BCL2, ERBB2), and α-glucosidase, a key enzyme in glucose metabolism, were selected as receptors. Using catechins as ligands, molecular docking was performed using the CDOCKER semi-flexible docking method in Discovery Studio software. The binding energy (-CDOCKERENERGY, unit: kcal / mol) is a negative value; the lower the value, the more stable the binding between the ligand and the receptor and the stronger the affinity. Specific results are shown in Table 1. The selected proteins include two major antioxidant proteins, SOD1 and KEAP1; two anti-inflammatory proteins, IL6 and TNF; two anti-tumor proteins, BCL2 and ERBB2; and... αUsing glucosidase as the receptor and catechins as the ligand, molecular docking was performed to evaluate the binding ability of compounds to proteins and their mechanisms of action. Table 1 shows that catechins exhibited strong binding activity with all seven target proteins. This result indicates that catechins possess multi-target binding ability and may exert synergistic pharmacological activity by simultaneously acting on antioxidant, anti-inflammatory, antitumor, and glucose metabolism-related pathways. Therefore, the plant extracts rich in functional flavonoids have potential application value in the preparation of antioxidant, anti-inflammatory, metabolic regulating, and adjuvant antitumor drugs. The mass spectrum of catechins is shown below. Figure 5 As shown.

[0048] Table 1. Molecular docking binding energies of catechins with 7 key functional proteins

[0049] Example 7 The above-mentioned plant extracts rich in functional flavonoids are used in the preparation of antioxidant, anti-inflammatory, anti-tumor, and hypoglycemic drugs.

[0050] This embodiment uses molecular docking technology to evaluate the binding ability of dihydromyricetin, the main active ingredient in flavonoid extract, to various key functional proteins, in order to reveal its potential molecular mechanisms in antioxidation, anti-inflammation, antitumor, and hypoglycemic effects. Two major antioxidant proteins, SOD1 and KEAP1; two anti-inflammatory proteins, IL6 and TNF; and two antitumor proteins, BCL2 and ERBB2, were selected. α - Using glucosidase as the receptor and dihydromyricetin as the ligand, molecular docking was performed. Table 2 shows that dihydromyricetin exhibited strong binding activity with all seven proteins. These results indicate that the plant extract rich in functional flavonoids has potential application value in the preparation of antioxidant, anti-inflammatory, antitumor, and hypoglycemic drugs. The mass spectrum of dihydromyricetin is shown below. Figure 6 As shown.

[0051] Table 2. Molecular docking binding energies of dihydromyricetin with 7 key functional proteins.

[0052] Example 8 The above-mentioned plant extracts rich in functional flavonoids are used in the preparation of antioxidant, anti-inflammatory, hypoglycemic, and cardiovascular protective drugs.

[0053] This embodiment uses molecular docking technology to evaluate the binding ability of quercetin, the main active ingredient in flavonoid extract, to various key functional proteins, in order to reveal its potential molecular mechanisms in antioxidation, anti-inflammation, and hypoglycemia. Two major antioxidant proteins, SOD1 and KEAP1; two anti-inflammatory proteins, IL6 and TNF; and two anti-tumor proteins, BCL2 and ERBB2, were selected.α - Glucosidase acts as the receptor, and quercetin acts as the ligand for molecular docking. Table 3 shows that quercetin binds to the antioxidant protein SOD1, the anti-inflammatory protein TNF, and... α - All glucosidases exhibited strong binding activity. This result indicates that the plant extract rich in functional flavonoids has potential application value in the preparation of antioxidant, anti-inflammatory, and hypoglycemic drugs. The mass spectrum of quercetin is shown below. Figure 7 As shown.

[0054] Table 3. Molecular docking binding energies of quercetin with 7 key functional proteins

Claims

1. A method for preparing a plant extract rich in functional flavonoid compounds, characterized in that, Includes the following steps: Remove impurities from the calyxes of the bitter rose, dry them in a cool, ventilated place, and then pulverize them for later use. Weigh the powdered calyxes of the bitter rose, use ethanol solution as the extraction solvent, and extract under ultrasonic conditions. Extract twice, combine the supernatants, and you will get an extract rich in flavonoids.

2. The method for preparing plant extracts rich in functional flavonoids according to claim 1, characterized in that, Prepare according to the following steps: Step 1: Remove impurities from the calyxes of the bitter rose, dry them in a cool, ventilated place, and then crush them for later use; Step 2: Weigh out the calyx powder of bitter rose and extract it using ethanol solution as the extraction solvent. Extract the powder under the following conditions: ethanol concentration of 40%-50%, material-to-liquid ratio of 1:20-40 g / mL, ultrasonic temperature of 40-55℃, and ultrasonic time of 20-40 min. Step 3: Extract twice following the above steps, combine the supernatants to obtain an extract rich in flavonoids.

3. The method for preparing plant extracts rich in functional flavonoid compounds according to claim 2, characterized in that, In step 1, a high-speed universal pulverizer is used to pulverize the dried calyx into fine powder with a relatively uniform particle size of 60-80 mesh.

4. Plant extracts rich in functional flavonoids prepared by the method according to any one of claims 1-3.

5. The plant extract rich in functional flavonoid compounds according to claim 4, characterized in that, The plant extract contains abundant flavonoid glycosides and aglycones, including catechins, dihydromyricetin, and quercetin.

6. The application of the plant extract rich in functional flavonoids according to claim 4 in the preparation of antioxidant, anti-inflammatory, and metabolic regulating drugs.

7. The application of the plant extract rich in functional flavonoids according to claim 4 in the preparation of antioxidant, anti-inflammatory, anti-tumor, and hypoglycemic drugs.

8. The application of the plant extract rich in functional flavonoids according to claim 4 in the preparation of antioxidant, anti-inflammatory, and hypoglycemic drugs.

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