Adaptive dynamic reservoir, reservoir system and oligonucleotide synthesis system thereof

Abstract

This invention provides an adaptive dynamic reservoir, a reservoir system, and an oligonucleotide synthesis system thereof, including an adaptive dynamic reservoir, a reservoir system based on the adaptive dynamic reservoir, and an oligonucleotide synthesis system incorporating the adaptive dynamic reservoir. This invention has the advantages of being able to temporarily store monomers and activators exceeding column volume during the coupling step and participate in the cyclic step; and being able to drain them before the washing step for easy cleaning.

Description

Technical Field

[0001] This invention relates to the field of biosynthetic molecules, and particularly to an adaptive dynamic reservoir, a reservoir system, and an oligonucleotide synthesis system thereof. Background Technology

[0002] Oligonucleotides generally refer to linear polynucleotide fragments consisting of 2 to 10 nucleotide residues linked by phosphodiester bonds. There is no strict rule on the number of nucleotide residues. In many literatures, polynucleotide molecules containing 30 or even more nucleotide residues are also referred to as oligonucleotides.

[0003] Existing oligonucleotide synthesis systems employ multiple rotary valves, synthesis columns, and various instruments to achieve 3D synthesis in the following steps: deprotection, activation coupling, oxidation, and capping. ， up to 5 ， Directional chain extension. For example, patent publication number CN113000003B, entitled "Nucleic Acid Synthesis System-Level Method," involves a nucleic acid synthesizer and method, including an injection line, a three-way valve, a circulation switching valve, a synthesis column, and an outlet line. In use, the injection line is cleaned with a cleaning agent, then the activating reagent and monomer are cyclically coupled, followed by cleaning and oxidation and capping steps. Its disadvantage is that if the added monomer and activating agent exceed the current volume of the synthesis column, the column cannot be fully loaded. Therefore, during the coupling cycle, only the volume of monomer and activating agent within the synthesis column can be circulated, reducing coupling efficiency. Summary of the Invention

[0004] To overcome the shortcomings of the prior art, the present invention provides an adaptive dynamic reservoir, a reservoir system and an oligonucleotide synthesis system thereof, which can temporarily store monomers and activators exceeding column volume during the coupling step and participate in the recycling step; at the same time, it can be emptied before the washing step for easy cleaning.

[0005] To address the aforementioned technical problems, the first aspect of this invention discloses an adaptive dynamic liquid reservoir, comprising a column, with an upper flange and a lower flange respectively provided at the top and bottom of the column; the lower flange is provided with a solution inlet; the upper flange is provided with a solution outlet and a gas pipe interface; a piston is provided inside the column and moves up and down along the column; a through flow channel is provided on the piston, and the flow channel is connected to the solution outlet of the upper flange through a pipe.

[0006] In some embodiments, the tube between the flow channel penetrating the piston and the solution outlet of the upper flange is a spring tube.

[0007] In some embodiments, the top of the column is provided with a protrusion for placing a spring tube.

[0008] In some embodiments, a piston rod is connected to the piston, the piston rod is movable and sealed through the solution outlet of the upper flange, and a tube is provided inside the piston rod, one end of the tube is connected to the flow channel on the piston, and the other end of the tube passes through the piston rod.

[0009] The second aspect of this invention discloses a liquid storage system based on an adaptive dynamic liquid reservoir, comprising an adaptive dynamic liquid reservoir, wherein the solution inlet on the lower flange is connected to an inlet pipe, the gas pipe interface on the upper flange is connected to a gas pipe, the gas pipe is connected to an inlet pipe through a valve, and the gas pipe is connected to an outlet pipe through a valve; the pipe body is connected to the solution outlet of the upper flange and is connected to the outlet pipe.

[0010] In some embodiments, the air pipe is connected to the air inlet pipe and the air outlet pipe via a two-position five-way reversing valve or a two-position three-way valve.

[0011] In some embodiments, a pneumatic dual unit is provided on the air intake pipe.

[0012] In some embodiments, a back pressure valve is provided on the liquid outlet pipe.

[0013] The third aspect of this invention discloses an oligonucleotide synthesis system based on an adaptive dynamic reservoir, comprising one or more synthesis columns, each of which is provided with a top inlet pipe and a bottom outlet pipe; each of the top inlet pipes is connected to a first main inlet pipe via a valve, and a first inlet pump is provided on the first main inlet pipe; each of the bottom outlet pipes is connected to an outlet main pipe via a valve; an adaptive dynamic reservoir is provided on the outlet main pipe; the outlet end of the outlet main pipe is connected to a collection pipe via a valve; the first main inlet pipe is connected to a second main inlet pipe via an intermediate pipe; a valve is provided between the intermediate pipe and the second main inlet pipe, and a second inlet pump is provided on the intermediate pipe; the outlet end of the outlet main pipe is connected to the intermediate pipe via a valve.

[0014] In some embodiments, a first pressure sensor and / or an ultraviolet sensor and / or a conductivity sensor are provided on the main outlet pipe; a second pressure sensor is provided on the first inlet pipe.

[0015] In some embodiments, the first inlet main pipe and the outlet main pipe are connected by a valve.

[0016] In some embodiments, five synthesis columns are used. The first main inlet pipe is connected to the top inlet pipe of the synthesis column via a first six-position seven-way valve. The first main inlet pipe is connected to the inlet end of the first six-position seven-way valve, and the outlet end of the first six-position seven-way valve is connected to the top inlet pipe of the synthesis column. The bottom outlet pipe of the synthesis column is connected to the outlet main pipe via a second six-position seven-way valve. The bottom outlet pipe of the synthesis column is connected to the inlet end of the second six-position seven-way valve, and the outlet end of the second six-position seven-way valve is connected to the outlet main pipe. One outlet end of the first six-position seven-way valve is connected to one inlet end of the second six-position seven-way valve via a pipe.

[0017] In some embodiments, the main outlet pipe, intermediate pipe, collection pipe, and second main inlet pipe are connected by a two-position four-way valve.

[0018] In some embodiments, a single synthesis column is used; the top inlet pipe, the first main inlet pipe, the bottom outlet pipe, and the main outlet pipe of the synthesis column are connected by a two-position four-way valve; an adaptive dynamic reservoir is installed on the main outlet pipe; the main outlet pipe, the intermediate pipe, the collection pipe, and the second main inlet pipe are connected by a two-position four-way valve; the intermediate pipe is connected to the first main inlet pipe; and inlet pumps are respectively installed on the first main inlet pipe and the intermediate pipe.

[0019] In some embodiments, a back pressure valve is provided on the collection pipe.

[0020] The beneficial effects of this invention are: 1. The volume of the adaptive dynamic reservoir can be changed. When the gas is pressurized, the reservoir volume decreases. When it is connected to the atmosphere, the piston moves to adaptively add volume. The liquid entering the adaptive dynamic reservoir will not be discharged but stored to participate in the circulation.

[0021] 2. When more than 1 volume of monomer and activator are added in the coupling step, the adaptive dynamic reservoir temporarily stores them, thereby realizing the efficient coupling reaction and achieving high synthesis efficiency and material saving. Attached Figure Description

[0022] Figure 1 This is a schematic diagram of the adaptive dynamic liquid reservoir of the present invention.

[0023] Figure 2 This is a schematic diagram of the structure of Embodiment 1 of the adaptive dynamic liquid reservoir of the present invention. Figure 3 This is a schematic diagram of the structure of Embodiment 2 of the adaptive dynamic liquid reservoir of the present invention.

[0024] Figure 4 This is a schematic diagram of the liquid storage system of the present invention.

[0025] Figure 5This is a schematic diagram of the structure of the oligonucleotide synthesis system of the present invention, Example 3.

[0026] Figure 6 This is a schematic diagram of the structure of the oligonucleotide synthesis system of the present invention, Example 4. Detailed Implementation

[0027] The present invention will now be further described with reference to the accompanying drawings.

[0028] The technical content of the present invention is illustrated below through specific embodiments. Those skilled in the art can easily understand other advantages and effects of the present invention from the content disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments. Those skilled in the art can make various modifications and changes without departing from the spirit of the present invention.

[0029] Before detailing the specific embodiments of this disclosure, some terms used in this disclosure will be explained first.

[0030] Unless otherwise defined below, all technical and scientific terms used herein are intended to have the same meaning as commonly understood by one of ordinary skill in the art. References to technical terms herein refer to techniques commonly understood in the art, including variations or equivalent substitutions of techniques that are obvious to one of ordinary skill in the art. While it is believed that the following terms will be well understood by one of ordinary skill in the art, the following definitions are set forth to better explain the invention. When a superior product name appears herein, it is intended to refer to the corresponding superior product. All patents, published patent applications, and publications cited herein are incorporated herein by reference.

[0031] The terms “connection,” “link,” “coupled,” or “coupled” used in this article are not limited to direct connections; they also include indirect connections.

[0032] As used herein, "online monitoring" or "real-time monitoring" refers to the real-time detection of certain parameters or properties of the buffer solution, reaction fluid, or fluid exiting the flow reactor during the use of the chromatography system, such as pH, pressure, flow rate, and conductivity. Unlike offline detection or analysis, online or real-time monitoring provides immediate feedback on the detection results.

[0033] As used herein, the term "synthesis column" refers to a traditional fixed-volume synthesis column or a variable-volume synthesis column designed by our company, such as the variable-volume synthesis column involved in patent application number 202111283536.5, entitled "A Variable-Volume Synthesis Column and a Synthesis System Thereof"; or patent application number 2025109620775, entitled "An Adaptive Pressure Synthesis Column and a Synthesis System Thereof".

[0034] The positional terms "up," "down," "left," "right," "front," and "back" used in this article are determined based on the layout direction of the accompanying drawings in the specification. They are only used to indicate relative positional relationships. When the absolute position of the object being described changes, the relative positional relationship may also change accordingly.

[0035] Reference Figure 1 As shown, the adaptive dynamic liquid reservoir includes a column 1, with an upper flange 2 and a lower flange 3 at the top and bottom of the column 1, respectively. The lower flange 3 is provided with a solution inlet 4, and the upper flange 2 is provided with a solution outlet 5 and a gas pipe interface 6. A piston 7 is provided inside the column 1, which can move up and down along the inner wall of the column 1. The piston 7 is provided with a through flow channel 8, which is connected to the solution outlet 5 through a pipe 9.

[0036] In use, piston 7 divides column 1 into upper and lower chambers. When the lower chamber needs to discharge solution, piston 7 moves downward under air pressure, and the solution in the lower chamber flows through flow channel 8 on piston 7, through pipe 9, and out through solution outlet 5. When piston 7 is placed at the bottom of column 1, flush with lower flange 3, solution inlet 4, flow channel 8, pipe 9, and solution outlet 5 are connected, thus realizing the function of a pipeline. When the lower chamber needs to store solution, air pipe interface 6 connects to the outside, and piston 7 moves upward as solution enters the lower chamber through solution inlet 4.

[0037] Example 1

[0038] Reference Figure 2 The adaptive dynamic liquid reservoir shown includes a column 21, with an upper flange 22 and a lower flange 23 at its top and bottom, respectively. A solution inlet 24 is provided on the lower flange 23. A solution outlet 25 and a gas pipe interface 26 are provided on the upper flange 22. A piston 27, which moves up and down along the inner wall of the column 21, is provided on the column 21, dividing the column 21 into two non-communicating cavities. A piston rod 210 is connected to the piston 27, passing through the solution outlet 25, and the piston rod 210 provides a movable seal with the upper flange 22. A flow channel 28 is provided on the piston 27. A tube 29 is provided inside the piston rod 210, with one end of the tube 29 connected to the flow channel 28, and the tube 29 passing through the solution outlet 25 via the piston rod 210.

[0039] In use, when the lower chamber needs to discharge solution, piston 27 moves downward under air pressure. At this time, the piston rod 210 and upper flange 22 also move in a sealed manner. The piston rod 210 serves to fix and guide the downward movement, allowing the solution in the lower chamber to be discharged through the flow channel 28 on piston 27 and the pipe 29. When piston 27 is positioned at the bottom of column 21, flush with lower flange 23, solution inlet 24, flow channel 28, and pipe 29 are connected, thus realizing the function of a pipeline. When the lower chamber needs to store solution, air inlet 26 connects to the outside. When solution enters the lower chamber through solution inlet 24, piston 27 moves upward.

[0040] Example 2

[0041] Reference Figure 3 As shown, the adaptive dynamic liquid reservoir includes a column 31. An upper flange 32 and a lower flange 33 are respectively provided at the top and bottom of the column 31. A solution inlet 34 is provided on the lower flange 33, and a solution outlet 35 and a gas pipe interface 36 are provided on the upper flange 32. A piston 37, movable up and down along the inner wall of the column 31, is disposed inside the column 31. A through-flow channel 38 is provided on the piston 37, which connects to the solution outlet 35 via a tube 39. The tube 39 is a spring tube, and the column 31 has a convex structure. The protrusion at the top is used to house the spring tube, preventing it from swaying left and right and improving the stability of the piston 37's up and down movement.

[0042] Reference Figure 4 The liquid storage system of the liquid reservoir shown includes an adaptive dynamic liquid reservoir 41. A solution inlet 44 on the lower flange 43 of the adaptive dynamic liquid reservoir 41 is connected to an inlet pipe 411. An air pipe interface 46 on the upper flange 42 of the adaptive dynamic liquid reservoir 41 is connected to an air pipe 412. The air pipe 412 is connected to port 2 of a two-position five-way valve 413. The air inlet 1 of the two-position five-way valve 413 is connected to an air inlet pipe 414. A pneumatic double-acting unit 415 is installed on the air inlet pipe 414. A pipe body 49 inside the adaptive dynamic liquid reservoir 41 is connected to an outlet pipe 416 through a solution outlet 45 on the upper flange 42. A back pressure valve 417 is installed on the outlet pipe 416.

[0043] In use, when liquid storage is required, the adaptive dynamic reservoir 41 switches to Vent mode. At this time, the two-position five-way valve 413 switches, with ports 1 and 4 connected, and ports 2 and 3 connected. The upper chamber of the adaptive dynamic reservoir 41 is connected to the atmosphere via the air pipe 412. The solution enters the lower chamber of the adaptive dynamic reservoir 41 through the inlet pipe 411. Because a back pressure valve 417 is installed on the outlet pipe 416, the piston 47 inside the adaptive dynamic reservoir 41 adaptively moves upward as the pumped solution enters, and the volume of the lower chamber adaptively equals the volume of the added solution. When the solution in the lower chamber needs to be drained, the adaptive dynamic reservoir 41 switches to pressurization mode. At this time, the two-position five-way valve 413 switches, connecting ports 1 and 2, thus opening the gas path. Gas enters the upper chamber of the adaptive dynamic reservoir 41 through the inlet pipe 414 and the gas pipe 412, pressurizing it. Simultaneously, the piston 47 moves downwards. When the inlet pipe 411 is blocked, the solution flows through the flow channel 48 on the piston 47, the pipe body 49, and the solution outlet 45 on the upper flange 42, exiting through the outlet pipe 416. Finally, the piston 47 is flush with the lower flange 43, achieving zero volume within the column and emptying the reservoir. With the solution inlet 44, flow channel 48, pipe body 49, and solution outlet 45 connected, the pipeline function is achieved.

[0044] Example 3

[0045] Reference Figure 5 The oligonucleotide synthesis system based on an adaptive dynamic reservoir shown includes five synthesis columns 61. Each synthesis column 61 has a top inlet pipe 62 and a bottom outlet pipe 63 at its upper and lower ends, respectively. The top inlet pipe 62 is connected to the outlet end of a first six-position seven-way valve 64, and the inlet end of the first six-position seven-way valve 64 is connected to a first main inlet pipe 65. A first inlet pump 66 and a second pressure sensor 620 are installed on the first main inlet pipe 65. The bottom outlet pipe 63 is connected to the inlet end of a second six-position seven-way valve 67, and the outlet end of the second six-position seven-way valve 67 is connected to an outlet main pipe 68. One outlet end of the first six-position seven-way valve 64 is connected to one inlet end of the second six-position seven-way valve 67 via a connecting pipe 69. An adaptive dynamic reservoir 610, a first pressure sensor 611, an ultraviolet sensor 612, and a conductivity sensor 613 are installed on the outlet main pipe 68. An adaptive dynamic liquid reservoir 610 is connected in series with the main outlet pipe 68 via a solution inlet and a solution outlet. The main outlet pipe 68, intermediate pipe 614, collection pipe 615, and second main inlet pipe 616 are connected via a first two-position four-way valve 619. A second inlet pump 617 is installed on the intermediate pipe 614. A back pressure valve 618 is installed on the collection pipe 615.

[0046] In use, the first two-position four-way valve 619 switches to connect the main outlet pipe 68 and the collection pipe 615, while the intermediate pipe 614 connects to the second main inlet pipe 616. Monomer and activator are added to the first main inlet pipe 65 and the second main inlet pipe 616, respectively. Under the action of the first inlet pump 66 and the second inlet pump 617, they enter one of the synthesis columns 61 through the top inlet pipe 62, and then enter the main outlet pipe 68 through the bottom outlet pipe 63 of the synthesis column 61. When the added monomer and activator exceed the volume of the synthesis column 61, the adaptive dynamic reservoir 610 switches to Vent mode. Because a back pressure valve 618 is installed on the collection pipe 615, the solution will not enter the collection pipe 615. After sample addition is complete and a cyclic reaction is required, the first two-position four-way valve 619 switches to connect the main outlet pipe 68 with the intermediate pipe 614, and the collection pipe 615 connects to the second main inlet pipe 616. At this time, the adaptive dynamic reservoir 610 is still in Vent mode, the first inlet pump 66 stops working, and the second inlet pump 617 operates. The solution stored in the adaptive dynamic reservoir 610 also participates in the solution circulation. All the solution forms a circulation loop through the intermediate pipe 614, the first main inlet pipe 65, the synthesis column 61, and the main outlet pipe 68.

[0047] After the cyclic reaction is completed, the first two-position four-way valve 619 switches to connect the main outlet pipe 68 and the collection pipe 615, and the intermediate pipe 614 connects to the second main inlet pipe 616. At this time, the adaptive dynamic reservoir 610 is switched to pressurization mode, the first inlet pump 66 and the second inlet pump 617 stop working, and the solution in the adaptive dynamic reservoir 610 is collected through the main outlet pipe 68 and the collection pipe 615. When the piston inside the adaptive dynamic reservoir 610 is flush with the lower flange, the column is empty, and the system is emptied. The solution inlet, flow channel, pipe body, and solution outlet inside the adaptive dynamic reservoir 610 are connected, and the pipeline function is realized. At this time, the first inlet pump 66 and the second inlet pump 617 are opened, and the cleaning solution flows in through the first main inlet pipe 65, the second main inlet pipe 616, and the intermediate pipe 614, passes through the synthesis column 61 and the main outlet pipe 68, and is collected by the collection pipe 615.

[0048] Example 4

[0049] Reference Figure 6The oligonucleotide synthesis system based on an adaptive dynamic reservoir shown includes a synthesis column 71, a top inlet pipe 72 and a bottom outlet pipe 73 at both ends of the synthesis column 71, a first main inlet pipe 74, and a main outlet pipe 75. The top inlet pipe 72, the bottom outlet pipe 73, the first main inlet pipe 74, and the main outlet pipe 75 are connected by a second two-position four-way valve 76. An adaptive dynamic reservoir 76, a first pressure sensor 77, an ultraviolet sensor 78, and a conductivity sensor 79 are installed on the main outlet pipe 75. The adaptive dynamic reservoir 76 is connected in series to the main outlet pipe 75 through a solution inlet and a solution outlet. The main outlet pipe 75, the intermediate pipe 710, the collection pipe 711, and the second main inlet pipe 712 are connected by a first two-position four-way valve 713. A first inlet pump 714 and a second pressure sensor 715 are installed on the first main inlet pipe 74. A back pressure valve 716 is installed on the collection pipe 711. A second inlet pump 717 is installed on the intermediate pipe 710.

[0050] In use, the second two-position four-way valve 76 switches to connect the first inlet main pipe 74 to the top inlet pipe 72, and the bottom outlet pipe 73 to the outlet main pipe 75. The first two-position four-way valve 713 switches to connect the outlet main pipe 75 to the collection pipe 711, and the intermediate pipe 710 to the second inlet main pipe 712. Monomers and activators are added to the first inlet main pipe 74 and the second inlet main pipe 712, respectively. Under the action of the first inlet pump 714 and the second inlet pump 717, they enter the synthesis column 71 through the top inlet pipe 72, and enter the outlet main pipe 75 through the bottom outlet pipe 73 of the synthesis column 71. When the added monomers and activators exceed the volume of the synthesis column 71, the adaptive dynamic reservoir 76 switches to Vent mode. Because a back pressure valve 716 is installed on the collection pipe 711, the solution will not enter the collection pipe 711. After sample addition is complete and a cyclic reaction is required, the first two-position four-way valve 713 switches to connect the main outlet pipe 75 with the intermediate pipe 710, and the collection pipe 711 connects with the second main inlet pipe 712. At this time, the adaptive dynamic reservoir 76 is still in Vent mode, the first inlet pump 714 stops working, and the second inlet pump 717 operates. The solution stored in the adaptive dynamic reservoir 76 also participates in the solution circulation. All the solution forms a circulation loop through the intermediate pipe 710, the first main inlet pipe 74, the synthesis column 71, and the main outlet pipe 75.

[0051] After the cyclic reaction is completed, the first two-position four-way valve 713 switches to connect the main outlet pipe 75 and the collection pipe 711, and the intermediate pipe 710 connects to the second main inlet pipe 712. At this time, the adaptive dynamic reservoir 76 is switched to pressurization mode, the first inlet pump 714 and the second inlet pump 717 stop working, and the solution in the adaptive dynamic reservoir 76 is collected through the main outlet pipe 75 and the collection pipe 711. When the piston inside the adaptive dynamic reservoir 76 is flush with the lower flange, the column is empty, and the system is emptied. The solution inlet, flow channel, pipe body, and solution outlet inside the adaptive dynamic reservoir 76 are connected, and the pipeline function is realized. At this time, the first inlet pump 714 and the second inlet pump 717 are opened, and the cleaning solution flows in through the first main inlet pipe 74, the second main inlet pipe 712, and the intermediate pipe 710, passes through the synthesis column 71 and the main outlet pipe 75, and is collected by the collection pipe 711.

[0052] When the second two-position four-way valve 76 switches to connect the first inlet main pipe 74 and the outlet main pipe 75, the top inlet pipe 72 is connected to the bottom outlet pipe 73. The cleaning fluid can flow through the first inlet main pipe 74, the second inlet main pipe 712, and the intermediate pipe 710, directly through the outlet main pipe 75, and be collected by the collection pipe 711.

Claims

1. An adaptive dynamic reservoir, characterized in that, The device includes a column, with an upper flange at the top and a lower flange at the bottom; the lower flange has a solution inlet; the upper flange has a solution outlet and a gas pipe interface; a piston that moves up and down along the column is installed inside the column; the piston has a through flow channel that connects to the solution outlet of the upper flange through a pipe.

2. The adaptive dynamic reservoir according to claim 1, characterized in that, The tube between the flow channel penetrating the piston and the solution outlet of the upper flange is a spring tube.

3. The adaptive dynamic reservoir according to claim 2, characterized in that, The top of the column is provided with a protrusion for placing the spring tube.

4. The adaptive dynamic reservoir according to claim 1, characterized in that, The piston is connected to a piston rod, which is movable and sealed through the solution outlet of the upper flange. A tube is installed inside the piston rod, with one end of the tube connected to the flow channel on the piston and the other end of the tube passing through the piston rod.

5. A liquid storage system based on an adaptive dynamic liquid reservoir, characterized in that, The adaptive dynamic liquid reservoir includes any one of claims 1-4, wherein the solution inlet on the lower flange is connected to the inlet pipe, the gas pipe interface on the upper flange is connected to the gas pipe, the gas pipe is connected to the inlet pipe through a valve, and the gas pipe is connected to the outlet pipe through a valve; the pipe body is connected to the solution outlet of the upper flange and connected to the outlet pipe.

6. The liquid storage system based on an adaptive dynamic liquid reservoir according to claim 5, characterized in that, The air pipe is connected to the air inlet pipe and the air outlet pipe via a two-position five-way reversing valve or a two-position three-way valve.

7. The liquid storage system based on an adaptive dynamic liquid reservoir according to claim 5, characterized in that, The air intake pipe is equipped with a pneumatic dual unit.

8. The liquid storage system based on an adaptive dynamic liquid reservoir according to claim 5, characterized in that, A back pressure valve is installed on the liquid outlet pipe.

9. An oligonucleotide synthesis system based on an adaptive dynamic reservoir, characterized in that, The invention comprises the adaptive dynamic reservoir as described in any one of claims 1-4, and one or more synthesis columns; each synthesis column is provided with a top inlet pipe and a bottom outlet pipe; each top inlet pipe is connected to a first main inlet pipe via a valve, and a first inlet pump is provided on the first main inlet pipe; each bottom outlet pipe is connected to an outlet main pipe via a valve; an adaptive dynamic reservoir is provided on the outlet main pipe; the outlet end of the outlet main pipe is connected to a collection pipe via a valve; the first main inlet pipe is connected to a second main inlet pipe via an intermediate pipe; a valve is provided between the intermediate pipe and the second main inlet pipe, and a second inlet pump is provided on the intermediate pipe; the outlet end of the outlet main pipe is connected to the intermediate pipe via a valve.

10. The oligonucleotide synthesis system based on an adaptive dynamic reservoir according to claim 9, characterized in that, The main outlet pipe is equipped with a first pressure sensor, an ultraviolet sensor, or a conductivity sensor; the main inlet pipe is equipped with a second pressure sensor.

11. The oligonucleotide synthesis system based on an adaptive dynamic reservoir according to claim 9, characterized in that, The first inlet main pipe and the outlet main pipe are connected by a valve.

12. The oligonucleotide synthesis system based on an adaptive dynamic reservoir according to claim 9, characterized in that, The synthesis column comprises five components. The first main inlet pipe is connected to the top inlet pipe of the synthesis column via a first six-position seven-way valve. The first main inlet pipe is connected to the inlet end of the first six-position seven-way valve, and the outlet end of the first six-position seven-way valve is connected to the top inlet pipe of the synthesis column. The bottom outlet pipe of the synthesis column is connected to the outlet main pipe via a second six-position seven-way valve. The bottom outlet pipe of the synthesis column is connected to the inlet end of the second six-position seven-way valve, and the outlet end of the second six-position seven-way valve is connected to the outlet main pipe. One outlet end of the first six-position seven-way valve is connected to one inlet end of the second six-position seven-way valve via a pipe.

13. The oligonucleotide synthesis system based on an adaptive dynamic reservoir according to claim 9, characterized in that, The main outlet pipe, intermediate pipe, collection pipe, and second main inlet pipe are connected by a two-position four-way valve.

14. The oligonucleotide synthesis system based on an adaptive dynamic reservoir according to claim 9, characterized in that, The synthesis column is a single unit; the top inlet pipe, the first main inlet pipe, the bottom outlet pipe, and the main outlet pipe of the synthesis column are connected by a two-position four-way valve. An adaptive dynamic reservoir is installed on the main outlet pipe. The main outlet pipe, the intermediate pipe, the collection pipe, and the second main inlet pipe are connected by a two-position four-way valve. The intermediate pipe is connected to the first main inlet pipe. Inlet pumps are installed on the first main inlet pipe and the intermediate pipe, respectively.

15. The oligonucleotide synthesis system based on an adaptive dynamic reservoir according to claim 9, characterized in that, A back pressure valve is installed on the collection pipe.

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