Method for efficiently inducing peony underground bud polyploidy and application thereof

By implanting photothermal-responsive slow-release microspheres into the side of peony underground buds, combined with photothermal regulation and graphene quantum dots, efficient and low-damage peony polyploid induction was achieved. This solved the problems of low induction efficiency and resource waste in traditional methods, and improved the homozygous polyploid rate and drug utilization rate. It is suitable for breeding ornamental, medicinal and stress-resistant peonies.

CN122095985APending Publication Date: 2026-05-29BOZHOU VOCATIONAL & TECHNICAL COLLEGE
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Patent Information

Application Number
CN202610297727.3
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-12
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing peony polyploid induction techniques suffer from problems such as low induction rate, high chimerism rate, low drug utilization rate, severe bud damage, and difficulty in screening homozygous polyploids. Furthermore, traditional methods are difficult to apply precisely to underground buds, leading to resource waste and environmental pollution.

Method used

Using photothermal-responsive sustained-release microsphere carriers, microspheres loaded with a composite inducer are implanted through a minimally invasive incision on the side of the peony underground bud. Combined with photothermal regulation, the drug can be released in a controlled manner. Graphene quantum dots are used to improve drug permeability and cell enrichment. Combined with cell synchronization treatment and polyploid identification and screening, the operation process is simplified.

Benefits of technology

It increases the homozygous polyploid rate to over 80%, reduces the chimeric rate to below 12%, improves bud survival rate and drug utilization, simplifies the operation process, conforms to the concept of green breeding, and is suitable for large-scale field breeding.

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Abstract

The invention relates to the technical field of plant breeding, and discloses a method for efficiently inducing peony underground bud polyploidy and application of the method, the method comprises the steps of underground bud disinfection, cell synchronization, minimally invasive pretreatment, CS-Alg / PPy photo-thermal response sustained release microsphere preparation, composite inducer loading, microsphere implantation and photo-thermal regulation induction, and polyploidy identification screening and transplanting, the composite inducer comprises colchicine, 6-BA and graphene quantum dots, and can block chromosome separation, realize cell chromosome doubling, regulate and control growth point cell division, repair cell damage, improve bud survival rate, assist photo-thermal regulation and control to realize precise slow release of drugs, and promote excellent character expression of polyploidy plants. The method is high in inductivity, homozygosity and bud survival rate and suitable for cultivation of ornamental, cut-flower, medicinal and stress-resistant peony, and the technology is advanced and practical.
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