An apparatus and method for tissue culture of figs

Abstract

The application relates to the technical field of plant tissue culture, and particularly discloses a fig tissue culture device and a culture method, which comprises a transparent cover body, a container and a culture plate. The container is internally provided with a plurality of containing grooves, the orientations of all containing groove openings are consistent, one side of the culture plate is provided with convex blocks, the number of which is not less than that of the containing grooves, the other side of the culture plate is provided with culture grooves corresponding to the positions of each convex block, and the bottom of each culture groove is provided with a plurality of through holes. The fig tissue culture device avoids cross-area transmission of bacterial contamination, improves the safety of the fig tissue culture device and the success rate of explant tissue culture, the fig tissue culture method realizes batch replacement of culture liquid, avoids bacterial contamination of the explant from external air, and improves the survival rate of long-period culture of the explant.

Description

Technical Field

[0001] This invention relates to the field of seed and seedling cultivation technology, and more particularly to the field of plant tissue culture technology, specifically a fig tissue culture device and culture method. Background Technology

[0002] Figs are mainly propagated asexually through cuttings and layering. Because figs have long relied on asexual propagation, once the mother plant is infected with a virus, its offspring will inevitably be infected as well, seriously affecting the growth of the fig plant and the quality of the fruit.

[0003] Plant tissue culture can enable the rapid propagation and virus-free rejuvenation of fig seedlings. By culturing shoot tip meristems, high-quality virus-free seedlings can be obtained, while breaking the limitations of seasonal and climatic conditions on fig propagation.

[0004] Current tissue culture mainly uses solid culture media, which involves adding solidifying agents such as agar to the culture medium to allow explants to grow on the surface of the solid medium. However, solid culture has problems such as long culture cycles and low propagation efficiency. Liquid tissue culture has the following advantages compared with existing solid culture: the explants and culture medium are in full contact, the utilization rate of nutrients is high, which is conducive to the growth and proliferation of explants. However, when pathogens contaminate the culture medium, they can spread rapidly in the culture medium and contaminate the entire culture container in a short time. Unlike the contamination on solid culture media, which is limited to the area around the inoculation point, contamination in liquid culture will be evenly distributed throughout the culture container, leading to the scrapping of the entire culture batch. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the existing defects and provide a fig tissue culture device and a culture method. The fig tissue culture device avoids the cross-regional spread of pathogen contamination, improves the safety of the fig tissue culture device and the success rate of explant tissue culture, and the fig tissue culture method enables batch replacement of culture medium, avoids explant contamination by pathogens in the external air, and improves the survival rate of explants in long-term culture.

[0006] The technical solution adopted by this invention to solve its technical problem includes: On the one hand, a fig tissue culture device is provided, including a transparent cover, a container and a culture plate, wherein the container is provided with a plurality of receiving slots, and the openings of all receiving slots face the same direction.

[0007] One side of the culture plate is provided with a number of protrusions no less than the number of receiving slots, and the other side of the culture plate is provided with a culture tank corresponding to the position of each protrusion. Each culture tank has several through holes at the bottom.

[0008] The container has an opening that connects to the receiving groove, and the opening is connected to one end of the vent pipe.

[0009] The opening of the accommodating slot faces upward, the culture plate is placed on the container, and at least one protrusion extends into each accommodating slot. The bottom side of the culture plate and the top side of the container are in sealed contact. Any two accommodating slots are connected through the gap between the bottom side of the culture plate and the top side of the container. The culture plate is provided with a transparent cover that covers all the culture slots.

[0010] As a preferred embodiment of the present invention, it also includes a restraint member for securing the transparent cover and the container together.

[0011] As a preferred embodiment of the present invention, the transparent cover has an installation hole that connects the inside and outside of it, and a filter membrane is installed in the installation hole.

[0012] As a preferred embodiment of the present invention, each protrusion is fixed with an extension tube at a position away from the culture tank, and the inner cavity of the extension tube and the culture tank are connected through the through hole.

[0013] On the other hand, another fig tissue culture device is also provided, including a transparent cover, a container and a culture plate. The container is provided with a plurality of receiving slots. The plurality of receiving slots are arranged in at least two columns spaced apart along the transverse direction of the container, and the plurality of receiving slots are arranged in at least two rows spaced apart along the vertical direction of the container. The openings of all receiving slots face the same direction.

[0014] Each receiving tank is equipped with a dividing frame that divides the receiving tank into two or four dividing chambers, and each dividing chamber is fixed with a liquid guide tube.

[0015] One side of the culture plate is provided with a number of protrusions equal to the number of accommodating slots, and the other side of the culture plate is provided with a culture slot corresponding to the position of each protrusion. Each culture slot has several through holes at the bottom.

[0016] The container has an opening that connects to the receiving groove, and the opening is connected to one end of the vent pipe.

[0017] The container has an upward-facing accommodating slot, and the culture plate is placed on the container. Each accommodating slot is provided with a corresponding protrusion. The bottom side of the culture plate and the top side of the container are in sealed sliding contact. Any two accommodating slots are connected through the gap between the bottom side of the culture plate and the top side of the container. The culture plate is provided with a transparent cover that covers all the culture slots.

[0018] The bottom side of the protrusion and the top of a liquid guide tube in the accommodating groove slide and seal against each other, pushing the culture plate to slide a set distance along a set direction against the container, and the bottom side of the protrusion and the top of another liquid guide tube in the accommodating groove slide and seal against each other.

[0019] As a preferred embodiment of the present invention, each receiving tank is provided with a partition, and a connecting hole is provided on the partition corresponding to the top of each liquid guide tube, and each connecting hole is connected to a liquid guide tube.

[0020] Wherein, after the bottom side of the culture plate and the top side of the container are in sealed sliding contact, the bottom side of the protrusion and the top side of the partition are in sealed sliding contact.

[0021] As a preferred embodiment of the present invention, a limiting member is installed on the transparent cover, container, or culture plate to restrict the movement of the culture plate.

[0022] As a preferred embodiment of the present invention, the transparent cover has an installation hole that connects the inside and outside of it, and a filter membrane is installed in the installation hole.

[0023] As a preferred embodiment of the present invention, it further includes a restraining member placed on the transparent cover, wherein two fixing members are symmetrically fixed on the container, and the two ends of the restraining member are respectively connected to the two fixing members.

[0024] Furthermore, a method for fig tissue culture is also provided, applicable to any of the aforementioned fig tissue culture devices, comprising the following steps: Culture solutions required for different developmental stages of explants were injected into each compartment of the container, and fig explants were placed in each culture tank of the culture plate.

[0025] Place the culture plate on the container, making sure the bottom of the protrusion and the top of a liquid guide tube in the accommodating groove slide and seal against each other, then cover it with the transparent cap.

[0026] Purified air is intermittently introduced into the containment tank through a vent pipe.

[0027] During the inflation process, the air pressure in the container is raised to the set air pressure range and maintained for a set time. Then, inflation is stopped or the air is vented through the venting pipe, and the air pressure in the container drops. The time interval between two inflations is 3-6 hours, which realizes intermittent immersion culture of explants.

[0028] Push the culture plate to slide a set distance along the set direction against the container. The bottom side of the protrusion and the top of another liquid guide tube in the accommodating groove slide to seal and abut against each other, and replace the culture medium that intermittently immerses the explant.

[0029] Compared with the prior art, the beneficial effects of the present invention are: 1. The fig tissue culture device of the present invention has independent compartments, and the explants in each compartment only come into contact with the culture medium in the corresponding compartment, which avoids the cross-regional spread of pathogen contamination and improves the safety of the fig tissue culture device and the success rate of explant tissue culture.

[0030] 2. The fig tissue culture device of the present invention can culture explants for different experimental treatments. Different types or concentrations of hormones and / or antibiotics can be added to different containers as needed without causing mutual mixing and interference. Harmful substances produced by the metabolism of a single explant or chemical substances secreted are confined to an independent culture medium and will not affect other explants, thus ensuring the precision of culture conditions.

[0031] 3. The fig tissue culture device of the present invention enables batch replacement of culture medium without opening the transparent cover and culture plate, avoiding contamination of explants by pathogens in the external air and improving the survival rate of explants in long-term culture.

[0032] 4. The fig tissue culture method of the present invention eliminates the steps of transferring a large number of culture devices to a clean bench, then opening the lid one by one, and moving the explants, thereby reducing the labor intensity and time cost of manual operation. Attached Figure Description

[0033] Figure 1 This is a schematic diagram of an embodiment of the present invention; Figure 2 for Figure 1 A schematic diagram of the exploded structure; Figure 3 for Figure 1 A schematic diagram of a partial cross-sectional structure of a culture plate; Figure 4 This is a schematic diagram of another embodiment of the present invention; Figure 5 for Figure 4 A schematic diagram of the exploded structure; Figure 6 for Figure 4 A partial sectional view of the structure; Figure 7 for Figure 6 Enlarged schematic diagram of the structure at point A; Figure 8 This is a schematic diagram of the liquid guide tube and the dividing frame in their mating and installation state according to the present invention; Figure 9 This is a schematic diagram of the liquid guide tube and the dividing frame in their separated state according to the present invention.

[0034] In the diagram: 1. Filter membrane, 2. Transparent cover, 3. Restraint, 4. Container, 5. Ventilation tube, 6. Culture plate, 7. Culture tank, 8. Receptacle, 9. Fixing element, 10. Extension tube, 11. Limiting element, 12. Connecting hole, 13. Partition, 14. Divider, 15. Liquid guide tube, 16. Protrusion. Detailed Implementation

[0035] The technical solutions of the present invention will be clearly and completely described below with reference to the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. All other embodiments obtained by those skilled in the art based on the embodiments of the present invention without creative effort are within the scope of protection of the present invention.

[0036] Example 1: Please see Figures 1-3 This embodiment discloses a fig tissue culture device, including a transparent cover 2, a container 4 and a culture plate 6. The container 4 is provided with a plurality of receiving slots 8, and the openings of all receiving slots 8 face the same direction.

[0037] One side of the culture plate 6 is provided with a number of protrusions 16 not less than the number of receiving grooves 8, and the other side of the culture plate 6 is provided with a culture groove 7 corresponding to the position of each protrusion 16, and the bottom of each culture groove 7 is provided with several through holes.

[0038] The container 4 has an opening that connects to the receiving groove 8. The opening is connected to one end of the vent pipe 5, and the other end of the vent pipe 5 is connected to the outlet of an external air source. The external air source can fill the receiving groove 8 with purified air through the vent pipe 5, and the air in the receiving groove 8 can also be discharged through the vent pipe 5. The external air source is an elastic air bag, an air pump, or a compressed air tank. An external air filter for filtering pathogens is installed between the external air source and the vent pipe 5.

[0039] The opening of the accommodating groove 8 faces upward, the culture plate 6 is placed on the container 4, there is a gap between the bottom side of the culture plate 6 and the top side of the container 4, and at least one protrusion 16 extends into each accommodating groove 8. The bottom side of the culture plate 6 and the edge of the top side of the container 4 are in sealed contact. Any two accommodating grooves 8 are connected through the gap between the bottom side of the culture plate 6 and the top side of the container 4. The culture plate 6 is provided with a transparent cover 2 that covers all the culture grooves 7.

[0040] The working process and principle of this embodiment are as follows: Perform the following operations on a clean bench or in other sterile environment: First, inject culture medium into each container 8. The staff places fig explants into each culture tank 7 of the culture plate 6. Then, place the culture plate 6 on the container 4 so that the lower part of each protrusion 16 is immersed in the culture medium and the explants do not contact the culture medium. Then, cover the culture plate 6 with a transparent cover 2.

[0041] Staff intermittently fill the container 8 with purified air from an external air source through the ventilation pipe 5. During the filling process, the air pressure in the container 8 increases, and the air in the container 8 forces the culture medium in the container 8 to flow upward into the culture tank 7 through the perforation at the bottom of the culture tank 7, thereby immersing the explant and bringing the explant into contact with the culture medium. The culture medium in each culture tank 7 does not flow to the top side of the culture plate 6. When the filling is stopped or the air is vented through the ventilation pipe 5, the air pressure in the container 8 decreases, and the culture medium in the culture tank 7 flows back into the container 8 through the perforation at the bottom of the culture tank 7 under the action of gravity. The explant is exposed to the air again. The above operation is repeated to achieve intermittent immersion culture of the explant.

[0042] Each container 8 is independent of the others, and the explants in each culture tank 7 only come into contact with the culture medium in the corresponding container 8. When the culture medium in a certain container 8 is contaminated with pathogens, the source of contamination is confined to the independent container 8 and will not spread to the explants in other culture tanks 7 through the shared culture medium. This avoids the cross-regional spread of pathogen contamination and improves the safety of the fig tissue culture device and the success rate of explant tissue culture.

[0043] For explants requiring different experimental treatments, different types or concentrations of hormones and / or antibiotics can be added to different containers 8 as needed without causing mutual mixing or interference. Harmful substances produced by the metabolism of a single explant or chemical substances secreted are confined to an independent culture medium and will not affect other explants, ensuring the precision of culture conditions.

[0044] This fig tissue culture device uses intermittent aeration to allow the culture medium to flow intermittently between the container tank 8 and the culture tank 7. This allows the explants to obtain water and nutrients regularly while ensuring they have sufficient time to come into contact with air, avoiding hypoxia or vitrification caused by prolonged immersion. This is beneficial for the growth and differentiation of the explants.

[0045] Furthermore, the transparent cover 2 has an installation hole that connects its inside and outside. A filter membrane 1, commonly used in plant tissue culture containers, is installed in the installation hole. The filter membrane 1 allows air molecules to pass freely while blocking airborne pathogens, ensuring that the inside of the transparent cover 2 remains sterile for a long time. During intermittent immersion culture, the filter membrane 1 on the transparent cover 2 allows gas exchange, maintains the air pressure balance inside and outside the transparent cover 2, and prevents external airborne pathogens from entering the transparent cover 2.

[0046] Furthermore, the culture tank 7 is equipped with a support or filler to prevent the explant from directly contacting the bottom side of the culture tank 7, thus avoiding the explant from blocking the perforation at the bottom of the culture tank 7 and facilitating the flow of culture medium through the perforation.

[0047] Furthermore, the liquid level of the culture medium in the container 8 is lower than the opening on the container 4, the culture medium in the container 8 will not flow out through the vent pipe 5, and the top edge of the inner wall of the container 8 is lower than the top side of the container 4.

[0048] Example 2: like Figure 1 As shown, this embodiment discloses a fig tissue culture device, the structure of which is roughly the same as that of Embodiment 1. The difference is that this embodiment also includes a binding member 3 to fix the transparent cover 2 and the container 4 together.

[0049] The working process and principle of this embodiment are as follows: The restraint component 3 is a restraint rope or tape. The restraint component 3 fixes the transparent cover 2, the culture plate 6 and the container 4 together to prevent unpurified external air from entering the culture tank 7 or the container tank 8.

[0050] Example 3: like Figure 3 As shown, this embodiment discloses a fig tissue culture device, whose structure is roughly the same as that of Embodiment 1. The difference is that in this embodiment, each protrusion 16 is fixed with an extension tube 10 at a position away from the culture tank 7, and the inner cavity of the extension tube 10 is connected to the culture tank 7 through a through hole.

[0051] The working process and principle of this embodiment are as follows: The bottom side of the protrusion 16 is higher than the liquid level of the culture medium in the container 8. The external air source fills the container 8 with purified air through the air pipe 5, the air pressure in the container 8 increases, and the culture medium in the container 8 enters the culture tank 7 through the extension pipe 10 and the through hole at the bottom of the culture tank 7, and wets the explant in the culture tank 7.

[0052] The extension tube 10 allows the perforation at the bottom of the culture tank 7 to be immersed in the culture medium in the container tank 8 without being submerged. By designing the length or diameter of the extension tube 10, the timing and height at which the culture medium enters the culture tank 7 can be precisely controlled. For example, the culture medium in the container tank 8 can only wet the explant when the air pressure in the container tank 8 reaches the set value, thus achieving precise control of the culture medium level.

[0053] Example 4:

[0054] Please see Figures 4-7 This embodiment discloses a fig tissue culture device, including a transparent cover 2, a container 4 and a culture plate 6. The container 4 is provided with a plurality of receiving slots 8. The plurality of receiving slots 8 are arranged in at least two columns along the horizontal direction of the container 4 and at least two rows along the vertical direction of the container 4. The openings of all receiving slots 8 face the same direction.

[0055] Each receiving tank 8 is provided with a dividing frame 14 that divides the receiving tank 8 into two or four dividing cavities. The dividing cavities in the receiving tank 8 are all oriented in the same direction, and each dividing cavity is fixed with a liquid guide tube 15.

[0056] One side of the culture plate 6 is provided with a number of protrusions 16 equal to the number of accommodating grooves 8. On the other side of the culture plate 6, a culture groove 7 is provided corresponding to the position of each protrusion 16. Several through holes are opened at the bottom of each culture groove 7.

[0057] The container 4 has an opening that connects to the receiving groove 8. The opening is connected to one end of the vent pipe 5, and the other end of the vent pipe 5 is connected to the outlet of an external air source. The external air source can fill the receiving groove 8 with purified air through the vent pipe 5, and the air in the receiving groove 8 can also be discharged through the vent pipe 5. The external air source is an elastic air bag, an air pump, or a compressed air tank. An external air filter for filtering pathogens is installed between the external air source and the vent pipe 5.

[0058] In this container, the opening of the accommodating groove 8 of the container 4 faces upward, the culture plate 6 is placed on the container 4, and each accommodating groove 8 is provided with a corresponding protrusion 16. The bottom side of the culture plate 6 and the top side of the container 4 are in sealed sliding contact. Any two accommodating grooves 8 are connected through the gap between the bottom side of the culture plate 6 and the top side of the container 4. The culture plate 6 is provided with a transparent cover 2 that covers all the culture grooves 7.

[0059] The bottom side of the protrusion 16 and the top end of a liquid guide tube 15 in the accommodating groove 8 slide and seal against each other, pushing the culture plate 6 to slide a set distance along the set direction against the container 4. The bottom side of the protrusion 16 and the top end of another liquid guide tube 15 in the accommodating groove 8 slide and seal against each other.

[0060] The working process and principle of this embodiment are as follows: Perform the following operations on a clean bench or in other sterile environment: First, inject the culture medium required for different developmental stages of the explant into each compartment of the container 8. Place fig explants in each culture tank 7 of the culture plate 6. Then, place the culture plate 6 on the container 4 so that the bottom side of the protrusion 16 and the top of a liquid guide tube 15 in the container 8 slide and seal against each other, and the explants do not contact the culture medium. Then, cover it with the transparent cover 2.

[0061] Staff intermittently fill the container 8 with purified air from an external air source through the ventilation pipe 5. During the inflation process, the air pressure in the container 8 increases, and the air in the container 8 causes the culture medium in one of the compartments to flow upward into the culture tank 7 through the perforation at the bottom of the culture tank 7, thereby immersing the explant and making the explant and culture medium come into brief contact. The culture medium in each culture tank 7 does not flow to the top side of the culture plate 6. When inflation stops or air is vented through the ventilation pipe 5, the air pressure in the container 8 decreases, and the culture medium in the culture tank 7 flows back into the compartment in the container 8 through the perforation at the bottom of the culture tank 7 under the action of gravity. The explant is exposed to the air again. The above operation is repeated to achieve intermittent immersion culture of the explant.

[0062] The staff pushes the culture plate 6 to slide a set distance along the set direction against the container 4. The bottom side of the protrusion 16 and the top of another liquid guide tube 15 in the accommodating groove 8 slide and seal against each other, and the culture medium that intermittently immerses the explant is replaced.

[0063] The culture medium can be replaced in batches without opening the transparent cover 2 and the culture plate 6, avoiding contamination of explants by pathogens in the external air and improving the survival rate of explants in long-term culture. The staff does not need to move this fig tissue culture device to the clean bench multiple times. They only need to push the culture plate 6 to slide along the set direction to complete the batch replacement of the culture medium, reducing labor intensity and compatibility with automated production.

[0064] Each container 8 is independent of the others, and the explants in each culture tank 7 only come into contact with the culture medium in the corresponding container 8. When the culture medium in a certain container 8 is contaminated with pathogens, the source of contamination is confined to the independent container 8 and will not spread to the explants in other culture tanks 7 through the shared culture medium. This avoids the cross-regional spread of pathogen contamination and improves the safety of the fig tissue culture device and the success rate of explant tissue culture.

[0065] Furthermore, the culture tank 7 is equipped with a support or filler to prevent the explant from directly contacting the bottom side of the culture tank 7, thus avoiding the explant from blocking the perforation at the bottom of the culture tank 7 and facilitating the flow of culture medium through the perforation.

[0066] Furthermore, the liquid level of the culture medium in the container 8 is lower than the opening on the container 4, the culture medium in the container 8 will not flow out through the vent pipe 5, and the top edge of the inner wall of the container 8 is lower than the top side of the container 4.

[0067] Example 5: like Figures 5-9 As shown, this embodiment discloses a fig tissue culture device, whose structure is roughly the same as that of embodiment four. The difference is that each accommodating tank 8 in this embodiment is provided with a partition 13, and a connecting hole 12 is opened on the partition 13 corresponding to the top of each liquid guide tube 15. Each connecting hole 12 is connected to a liquid guide tube 15.

[0068] The working process and principle of this embodiment are as follows: After the bottom side of the culture plate 6 and the top side of the container 4 are in sealed sliding contact, the bottom side of the protrusion 16 and the top side of the partition 13 are in sealed sliding contact, and the partition 13 guides the movement of the protrusion 16.

[0069] Example 6: like Figures 4-6 As shown, this embodiment discloses a fig tissue culture device, whose structure is roughly the same as that of embodiment four. The difference is that in this embodiment, a limiting member 11 is installed on the transparent cover 2, the container 4 or the culture plate 6 to restrict the movement of the culture plate 6. The limiting member 11 is a clip, tape or a card holder with a slot.

[0070] The working process and principle of this embodiment are as follows: After the inner cavity of a liquid guide tube 15 in the culture tank 7 and the container tank 8 is connected, the limiting member 11 fixes the position of the culture plate 6 relative to the container 4.

[0071] Furthermore, the culture plate 6 is made of transparent plastic or transparent glass.

[0072] Example 7: like Figures 4-6 As shown, this embodiment discloses a fig tissue culture device, whose structure is roughly the same as that of Embodiment 4. The difference is that the transparent cover 2 in this embodiment has an installation hole that connects the inside and outside of it. A filter membrane 1, commonly used in plant tissue culture containers, is installed in the installation hole. The filter membrane 1 allows air molecules to pass freely and blocks pathogens in the outside air, ensuring that the inside of the transparent cover 2 is in a sterile state for a long time. During the intermittent immersion culture of explants, the filter membrane 1 on the transparent cover 2 allows gas exchange, maintains the air pressure balance inside and outside the transparent cover 2, and prevents external pathogens from entering the transparent cover 2.

[0073] Example 8: like Figures 4-6As shown, this embodiment discloses a fig tissue culture device, whose structure is roughly the same as that of Embodiment 1. The difference is that this embodiment also includes a restraint member 3 that is placed on the transparent cover 2. Two fixing members 9 are symmetrically fixed on the container 4. The two ends of the restraint member 3 are respectively connected to the two fixing members 9. The restraint member 3 is an elastic rubber rope or fiber rope, and the fixing member 9 is a fixing rod or a fixing ring.

[0074] The working process and principle of this embodiment are as follows: The two ends of the restraint 3 are connected to the two fixing parts 9 respectively, and the restraint 3 presses on the transparent cover 2 so that the culture plate 6 is always fastened to the container 4.

[0075] Example 9: like Figures 4-9 As shown, this embodiment discloses a fig tissue culture method, applied to the fig tissue culture apparatus of any one of Embodiments 4 to 8, comprising the following steps: Culture solutions required for different developmental stages of explants are injected into each segmented cavity of the receiving tank 8, and fig explants are placed in each culture tank 7 of the culture plate 6.

[0076] Place the culture plate 6 on the container 4, so that the bottom side of the protrusion 16 and the top of a liquid guide tube 15 in the accommodating groove 8 slide and seal against each other, and then cover it with the transparent cover 2.

[0077] Purified air is intermittently introduced into the receiving tank 8 through the vent pipe 5.

[0078] During the inflation process, the air pressure in the container 8 is raised to the set air pressure range and maintained for a set time. Then, inflation is stopped or the air is vented through the vent pipe 5, and the air pressure in the container 8 decreases. The time interval between two inflations is 3-6 hours, and the culture medium soaks the explants for 3-7 minutes, thus realizing intermittent immersion culture of the explants.

[0079] Push the culture plate 6 to slide a set distance along the set direction against the container 4. The bottom side of the protrusion 16 and the top of another liquid guide tube 15 in the accommodating groove 8 slide and seal against each other to replace the culture medium that intermittently immerses the explant.

[0080] The working process and principle of this embodiment are as follows: Batch replacement of culture medium can be achieved without opening the transparent cover 2 and culture plate 6, avoiding contamination of explants by airborne pathogens, improving the survival rate of explants in long-term culture, and eliminating the need to transfer a large number of culture devices to a clean bench, then open the cover one by one and move the explants, thus reducing the labor intensity and time cost of manual operation.

[0081] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention.

Claims

1. A fig tissue culture device, characterized in that: It includes a transparent cover (2), a container (4) and a culture plate (6). The container (4) is provided with a number of receiving slots (8), and the openings of all receiving slots (8) face the same direction. The culture plate (6) has a number of protrusions (16) on one side that is no less than the number of receiving grooves (8), and a culture groove (7) is provided on the other side of the culture plate (6) corresponding to the position of each protrusion (16). Each culture groove (7) has several through holes at the bottom. The container (4) has an opening that connects to the receiving groove (8), and the opening is connected to one end of the vent pipe (5). The opening of the accommodating groove (8) faces upward, the culture plate (6) is placed on the container (4), and at least one protrusion (16) extends into each accommodating groove (8). The bottom side of the culture plate (6) and the top side of the container (4) are in sealed contact. Any two accommodating grooves (8) are connected through the gap between the bottom side of the culture plate (6) and the top side of the container (4). The culture plate (6) is provided with a transparent cover (2) that covers all the culture grooves (7).

2. The fig tissue culture apparatus according to claim 1, characterized in that: It also includes a restraint (3) that secures the transparent cover (2) and the container (4) together.

3. The fig tissue culture apparatus according to claim 1, characterized in that: The transparent cover (2) has an installation hole that connects the inside and outside of it, and a filter membrane (1) is installed in the installation hole.

4. The fig tissue culture apparatus according to claim 1, characterized in that: Each protrusion (16) is fixed with an extension tube (10) at a position away from the culture tank (7), and the inner cavity of the extension tube (10) and the culture tank (7) are connected through the through hole.

5. A fig tissue culture device, characterized in that: It includes a transparent cover (2), a container (4) and a culture plate (6). The container (4) is provided with a plurality of receiving slots (8). The plurality of receiving slots (8) are arranged in at least two columns along the transverse direction of the container (4) and at least two rows along the vertical direction of the container (4). The openings of all receiving slots (8) face the same direction. Each receiving tank (8) is provided with a dividing frame (14) that divides the receiving tank (8) into two or four dividing cavities, and each dividing cavity is fixed with a liquid guide tube (15). The culture plate (6) has a number of protrusions (16) equal to the number of receiving grooves (8) on one side, and a culture groove (7) is provided on the other side of the culture plate (6) corresponding to the position of each protrusion (16). Each culture groove (7) has several through holes at the bottom. The container (4) has an opening that connects to the receiving groove (8), and the opening is connected to one end of the vent pipe (5). The container (4) has its accommodating slot (8) opening upwards, the culture plate (6) is placed on the container (4), and each accommodating slot (8) is provided with a corresponding protrusion (16). The bottom side of the culture plate (6) and the top side of the container (4) are in sealed sliding contact. Any two accommodating slots (8) are connected through the gap between the bottom side of the culture plate (6) and the top side of the container (4). The culture plate (6) is provided with a transparent cover (2) that covers all the culture slots (7). The bottom side of the protrusion (16) and the top end of a liquid guide tube (15) in the accommodating groove (8) slide and seal against each other, pushing the culture plate (6) to slide a set distance along the set direction against the container (4), and the bottom side of the protrusion (16) and the top end of another liquid guide tube (15) in the accommodating groove (8) slide and seal against each other.

6. The fig tissue culture apparatus according to claim 5, characterized in that: Each receiving tank (8) is provided with a partition (13), and a connecting hole (12) is provided on the partition (13) corresponding to the top of each liquid guide tube (15). Each connecting hole (12) is connected to a liquid guide tube (15). After the bottom side of the culture plate (6) and the top side of the container (4) are in sealed sliding contact, the bottom side of the protrusion (16) and the top side of the partition (13) are in sealed sliding contact.

7. The fig tissue culture apparatus according to claim 5, characterized in that: A limiting element (11) is installed on the transparent cover (2), the container (4), or the culture plate (6) to restrict the movement of the culture plate (6).

8. The fig tissue culture apparatus according to claim 5, characterized in that: The transparent cover (2) has an installation hole that connects the inside and outside of it, and a filter membrane (1) is installed in the installation hole.

9. The fig tissue culture apparatus according to claim 5, characterized in that: It also includes a restraint (3) that is placed on the transparent cover (2). Two fasteners (9) are symmetrically fixed on the container (4). The two ends of the restraint (3) are respectively connected to the two fasteners (9).

10. A method for fig tissue culture, applied to the fig tissue culture apparatus according to any one of claims 5-9, characterized in that, Includes the following steps: The culture medium required for different developmental stages of the explant is injected into each compartment of the container (8), and fig explants are placed in each culture tank (7) of the culture plate (6). Place the culture plate (6) on the container (4) so ​​that the bottom side of the protrusion (16) and the top of a liquid guide tube (15) in the accommodating groove (8) slide and seal against each other, and then cover it with a transparent cover (2). Purified air is intermittently introduced into the accommodating tank (8) through the ventilation pipe (5); During the inflation process, the air pressure in the container (8) is raised to the set air pressure range and maintained for a set time. Then, inflation is stopped or the air is vented through the vent pipe (5). The air pressure in the container (8) drops. The time interval between two inflations is 3-6 hours, thus achieving intermittent immersion culture. Push the culture plate (6) to fit the container (4) and slide it a set distance along the set direction. The bottom side of the protrusion (16) and the top of another liquid guide tube (15) in the accommodating groove (8) slide and seal against each other to replace the culture medium that intermittently immerses the explant.

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