A high-efficiency fritillaria unibractea bulb induction and culture method for alkaloid extraction

By optimizing the culture medium and disinfection process of Fritillaria thunbergii bulbs in Yuzhong, an efficient bulb regeneration system was established, solving the problem of low propagation efficiency of Fritillaria thunbergii in Yuzhong and realizing the efficient production of regenerated small bulbs and alkaloid extraction to meet market demand.

CN122095995APending Publication Date: 2026-05-29NINGXIA UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NINGXIA UNIVERSITY
Filing Date
2026-04-27
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

The low reproductive efficiency and long growth cycle of Fritillaria thunbergii in Yuzhong, coupled with the lack of a regeneration system and insufficient bulb resources for alkaloid extraction, make it difficult to meet market demand.

Method used

Bulbs of Fritillaria thunbergii were induced and cultured using MS medium with specific hormone ratios, including callus induction medium and regeneration medium. By optimizing the sterilization process and culture environment, regenerated small bulbs with uniform morphology and robust growth were induced, and the alkaloid content was tested to meet the medicinal standards.

Benefits of technology

It significantly improved the regeneration and cultivation efficiency of small bulbs, with a bulb induction rate of up to 90% and a proliferation coefficient of 7.08. The regenerated small bulbs obtained met the requirements for medicinal use, realizing a sustainable supply of medicine from Fritillaria thunbergii in Yuzhong.

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Abstract

The present application belongs to the technical field of plant tissue culture, and particularly relates to a Yuzhong Fritillaria bulb induction and culture method. The present application uses Yuzhong Fritillaria bulb as an explant, inoculates the explant into a callus induction medium to form callus and adventitious buds, inoculates the callus and adventitious buds into a regeneration medium to obtain regenerated bulbs; the callus induction medium is MS+0.5 mg / L 6-BA+1.0 mg / L NAA+0.2 mg / L TDZ or MS+0.5 mg / L 6-BA+1.0 mg / L IBA+0.2 mg / L TDZ; the regeneration medium is MS+0.5 mg / L 6-BA+1.0 mg / L NAA+0.2 mg / L TDZ; after 20 days of induction by the method, 5 to 15 regenerated small bulbs can be observed to be differentiated from one piece of callus, the induction rate of the regenerated small bulbs is more than 90%, the proliferation coefficient is 7.08, and the total alkaloid content of the regenerated small bulbs is 0.068%.
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