A copper-based hydrotalcite material loaded with abamectin material, and a preparation method and application thereof

Abstract

The application provides a kind of copper-based hydrotalcite material loading abamectin material and its preparation method and application, belongs to the technical field of pesticide manufacturing.The copper-based hydrotalcite is used as carrier to load abamectin pesticide, wherein the degradation product of copper-based hydrotalcite carrier in plant is nano copper oxide and metal copper ion, which can be used to generate active oxygen and synergize with abamectin fungicide.Especially in the occurrence site of fungal disease, the acid substances such as oxalic acid produced by pathogen can accelerate the degradation of copper-based hydrotalcite carrier, accelerate the transformation of hydrotalcite structure to nano copper oxide structure by responding to the environment pH, generate active oxygen to kill pathogen, so as to achieve the effect of sterilization.At the same time, the copper-based hydrotalcite carrier can mediate the transport of loaded abamectin pesticide to the plant, effectively improve the systemicity of abamectin pesticide, make it efficiently enriched in the main lesion site, and realize the precision control of disease and insect pest.

Description

Technical Field

[0001] This invention belongs to the field of pesticide manufacturing technology, specifically relating to a copper-based hydrotalcite material loaded with abamectin, its preparation method, and its application. Background Technology

[0002] Pesticides are essential agricultural inputs for controlling pests and diseases and ensuring food security. In actual agricultural production, multiple pests and diseases often occur simultaneously. For example, fungal diseases can cause plant tissue wilting, which then attracts pests, leading to further infestations. During their migration, pests can easily spread pathogens, causing simultaneous outbreaks of pests and diseases, greatly increasing the difficulty of control. Currently, the main approach to dealing with simultaneous outbreaks is through the repeated and large-scale application of multiple pesticides. This method not only easily leads to pesticide residues but also readily leaches into the soil and groundwater, posing significant environmental risks.

[0003] In existing technologies, the problem of simultaneous occurrence of pests and diseases is mainly solved by mixing and applying multiple pesticides. However, for some common copper-based pesticides, such as copper hydroxide water-dispersible granules and copper hydroxide 3000, because they do not have systemic properties, they mainly exert their therapeutic effect through contact killing after the disease has occurred. They cannot be effectively exerted through systemic transmission within the plant to achieve disease prevention. Summary of the Invention

[0004] The purpose of this invention is to provide a copper-based hydrotalcite material loaded with abamectin, its preparation method, and its application. The copper-based hydrotalcite material loaded with abamectin provided by this invention uses copper hydrotalcite as a carrier to effectively improve the systemic properties of abamectin pesticide, enabling it to accumulate efficiently at the main lesion sites and achieve precise control of pests and diseases.

[0005] To achieve the objectives of this invention, the following technical solutions are provided: A copper-based hydrotalcite material loaded with avermectin includes a copper-based hydrotalcite carrier and avermectin loaded on the surface and pores of the copper-based hydrotalcite carrier. The copper-based hydrotalcite carrier is a surfactant-modified copper-based hydrotalcite carrier; The chemical composition of the copper-based hydrotalcite carrier is Cu. a (M1) b (M2)1(OH)2Y c A d ·mH2O; where M1 is Mg 2+ Ca 2+ Zn 2+ and Ni 2+ One or more of them; M2 is Fe 3+ Cr 3+ And Al 3+One or more of the following; Y is a surfactant; A is NO3. - SO4 2- and Cl - One or more of them.

[0006] Preferably, a, b, c, d, and m are the molar percentages of Cu, M1, M2, Y, and A in the avermectin-loaded material per mole of copper-based hydrotalcite material; a = 1~4; b = 0~4; c = 0.0001~0.001; d = 1~20; a+b = 2~4; m = 3~20; and the chemical composition of the copper-based hydrotalcite carrier maintains charge conservation.

[0007] Preferably, the surfactant comprises one or more of sodium dodecyl sulfate, hexadecyltrimethylammonium bromide, and sodium stearate.

[0008] Preferably, the mass of the avermectin is 5-100% of the mass of the copper-based hydrotalcite carrier.

[0009] Preferably, the particle size of the copper-based hydrotalcite material loaded with avermectin is 20~300nm.

[0010] This invention also provides a method for preparing avermectin-loaded copper-based hydrotalcite material as described in the above technical solution, comprising the following steps: A soluble copper salt, a soluble M1 metal salt, a soluble M2 metal salt, an alkaline reagent, and a surfactant were mixed and subjected to a co-precipitation reaction to obtain a copper-based hydrotalcite carrier. Abamectin was loaded onto the copper-based hydrotalcite carrier in the liquid phase to obtain the copper-based hydrotalcite material loaded with abamectin.

[0011] Preferably, the coprecipitation reaction is carried out under shear conditions; the shear rate is 3000~5000 r / min, the time is 1~3 min, and the temperature is 25~40℃.

[0012] Preferably, the liquid phase loading is carried out under shear conditions, wherein the shear rate is 3000~5000 r / min, the time is 1~3 min, and the temperature is 25~40℃.

[0013] The present invention also provides the application of the copper-based hydrotalcite material loaded with abamectin as described in the above technical solution or the copper-based hydrotalcite material loaded with abamectin prepared by the preparation method described in the above technical solution in the prevention and control of fungal diseases or pests in crops.

[0014] Preferably, the fungal diseases include one or more of rice blast, root rot, stem rot and clubroot; the insect pests include diamondback moth and / or root-knot nematodes.

[0015] This invention provides a copper-based hydrotalcite material loaded with avermectin, comprising a copper-based hydrotalcite carrier and avermectin loaded on the surface and within the pores of the copper-based hydrotalcite carrier; the copper-based hydrotalcite carrier is a surfactant-modified copper-based hydrotalcite carrier; the chemical composition of the copper-based hydrotalcite carrier is Cu. a (M1) b (M2)1(OH)2Y c A d ·mH2O; where M1 is Mg 2+ Ca 2+ Zn 2+ and Ni 2+ One or more of them; M2 is Fe 3+ Cr 3+ And Al 3+ One or more of the following; Y is a surfactant; A is NO3. - SO4 2- and Cl - One or more of the following. In this invention, copper-based hydrotalcite is used as a carrier to load abamectin pesticide. The degradation products of the copper-based hydrotalcite carrier within the plant are nano-copper oxide and metallic copper ions, which can be utilized to synergistically enhance the effect of abamectin fungicide by utilizing the active oxygen generated. Especially at the site of fungal disease, acidic substances such as oxalic acid produced by pathogens accelerate the degradation of the copper-based hydrotalcite carrier. Through the response to the environmental pH, the transformation of the hydrotalcite structure into a nano-copper oxide structure is accelerated, generating active oxygen to kill pathogens and achieve a fungicidal effect. At the same time, the copper-based hydrotalcite carrier can mediate the transport of the loaded abamectin pesticide into the plant, effectively improving the systemic properties of the abamectin pesticide, allowing it to accumulate efficiently at the main lesion sites, and achieving precise control of pests and diseases.

[0016] Furthermore, in this invention, the surfactant in the copper-based hydrotalcite material loaded with abamectin is modified on the surface and between the hydrotalcite layers by hydrogen bonding to form hydrophobic spaces, thereby enabling the self-assembly of hydrophobic abamectin pesticide molecules and improving the stability of the copper-based hydrotalcite material loaded with abamectin during the absorption, conduction and transport process to various parts of the plant. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0018] Figure 1The XRD pattern, infrared spectrum, transmission electron microscope image, and elemental analysis spectrum of the abamectin copper hydrotalcite nanopesticide obtained in Example 1 of this invention are shown below. Figure 2 This is a transmission electron microscope (TEM) image of the distribution of the abamectin copper hydrotalcite nanopesticide obtained in Example 1 of the present invention within the plant and its interaction with the rice blast pathogen. Figure 3 The images show the pH-responsive reactive oxygen species (EPS) generation spectrum and XRD, XPS, transmission electron microscopy, and AFM images before and after the response of the abamectin copper hydrotalcite nanopesticide obtained in Example 1 of this invention. Figure 4 This is a comparison chart showing the abamectin content in plants 24 hours after application of the abamectin copper hydrotalcite nanopesticide obtained in Example 1 of this invention and the commercially available abamectin emulsifiable concentrate formulation. Detailed Implementation

[0019] This invention provides a copper-based hydrotalcite material loaded with avermectin, comprising a copper-based hydrotalcite carrier and avermectin loaded on the surface and within the pores of the copper-based hydrotalcite carrier; The copper-based hydrotalcite carrier is a surfactant-modified copper-based hydrotalcite carrier; The chemical composition of the copper-based hydrotalcite carrier is Cu. a (M1) b (M2)1(OH)2Y c A d ·mH2O; where M1 is Mg 2+ Ca 2+ Zn 2+ and Ni 2+ One or more of them; M2 is Fe 3+ Cr 3+ And Al 3+ One or more of the following; Y is a surfactant; A is NO3. - SO4 2- and Cl - One or more of them.

[0020] In this invention, a, b, c, d, and m represent the molar percentages of Cu, M1, M2, Y, and A in the avermectin-loaded material per mole of copper-based hydrotalcite material; I = 0~4; b = 0~4; c = 0.0001~0.001; d = 1~20; a+b = 2~4; and the chemical composition of the copper-based hydrotalcite carrier maintains charge conservation.

[0021] In this invention, the surfactant includes one or more of sodium dodecyl sulfate (SDS), hexadecyltrimethylammonium bromide, and sodium stearate, and in specific embodiments, it can be SDS. In this invention, the surfactant modifies the surface and interlayer of the hydrotalcite layer via hydrogen bonding to form hydrophobic spaces, enabling the self-assembly of hydrophobic avermectin pesticide molecules and improving the stability of the copper-based hydrotalcite material loaded with avermectin during absorption, conduction, and transport to various parts of the plant.

[0022] In this invention, the mass of avermectin is 5-100% of the mass of the copper-based hydrotalcite carrier, and in specific embodiments it can be 8%, 10%, 15%, 30%, 45%, 50%, 75%, or 80%. This invention increases the compatibility of the hydrophilic-hydrophobic self-assembly process by employing a matching solvent with excellent solubility and a modified surfactant, resulting in a high loading ratio of avermectin.

[0023] In this invention, the chemical composition of the copper-based hydrotalcite material loaded with avermectin can be Cu a (M1) b (M2)1(OH)2Y c A d ·mH2O / n(C 48 H 72 O 14 (B1a)·C 47 H 70 O 14 (B1b)); where M1, M2, Y, A, a, b, c, d are as defined above, and n = 1~0.05.

[0024] In this invention, the particle size of the copper-based hydrotalcite material loaded with abamectin is 20-300 nm, and in specific embodiments it can be 30, 50, 100, or 200 nm. By controlling the particle size of the copper-based hydrotalcite material loaded with abamectin within the above range, this invention ensures the effective absorption of nano-pesticides by plants while reducing degradation and soil leaching caused by excessively small particle sizes.

[0025] This invention also provides a method for preparing the copper-based hydrotalcite material loaded with abamectin as described in the above technical solution, including the following steps: A soluble copper salt, a soluble M1 metal salt, a soluble M2 metal salt, an alkaline reagent, and a surfactant were mixed and subjected to a co-precipitation reaction to obtain a copper-based hydrotalcite carrier. Abamectin was loaded onto the copper-based hydrotalcite carrier in the liquid phase to obtain the copper-based hydrotalcite material loaded with abamectin.

[0026] In this invention, unless otherwise specified, all raw materials used in the preparation are commercially available products well known to those skilled in the art.

[0027] In this invention, the soluble M1 metal salt is a soluble divalent metal salt, and the soluble M2 metal salt is a soluble trivalent metal salt; the soluble M1 metal salt is one or more of magnesium salt, calcium salt, and zinc salt; the soluble M2 metal salt is one or more of trivalent iron salt, trivalent chromium salt, and aluminum salt; the acid anions in the soluble metal salt solution are one or more of nitrate, sulfate, and hydrochloride ions, which in specific embodiments can be copper nitrate, magnesium nitrate, and aluminum nitrate, copper nitrate and aluminum nitrate, or copper nitrate, zinc nitrate, and aluminum nitrate; the alkaline reagent can be sodium hydroxide.

[0028] In this invention, the mixing order of the soluble copper salt, the soluble M1 metal salt, the soluble M2 metal salt, the alkaline reagent, and the dispersant can be as follows: Soluble copper salt, soluble M1 metal salt, soluble M2 metal salt and water are mixed to obtain solution A; The alkaline reagent and dispersant are mixed to obtain solution B; Solution A and solution B are mixed to carry out a coprecipitation reaction.

[0029] In this invention, the concentration of copper ions in solution A is 0.1~1 mol / L, and in specific embodiments it can be 0.2, 0.5, or 0.8 mol / L; the concentration of Mg, Ca, Zn, Fe, Cr, or Al is 0.1~1 mol / L, and in specific embodiments it can be 0.3, 0.6, or 0.8 mol / L; the concentration of the alkaline reagent in solution B is 0.01~1.0 mol / L, and in specific embodiments it can be 0.1, 0.3, 0.6, or 0.8 mol / L; the concentration of the dispersant is 0.001~0.01 mol / L, and in specific embodiments it can be 0.002, 0.005, or 0.007 mol / L. This invention adjusts the pH of the solution to 8.0~14.0 using the alkaline reagent to facilitate the coprecipitation reaction.

[0030] In this invention, the coprecipitation reaction is carried out under shear conditions; the shear rate is 3000~5000 r / min, the time is 1~3 min, and the temperature is 25~40℃. During the coprecipitation reaction of this invention, a copper-doped metal hydroxide structure modified with a surfactant is rapidly formed through the interaction between hydroxyl groups and metal salt ions.

[0031] In this invention, the coprecipitation reaction is further followed by centrifugation and washing, and there are no special limitations on centrifugation and washing in this invention.

[0032] In this invention, the avermectin is liquid-loaded in the form of an avermectin solution; the solvent of the avermectin solution is acetone, and the purity of the avermectin is ≥95%.

[0033] In this invention, the liquid phase loading is carried out under shear conditions, wherein the shear rate is 3000~5000 r / min, the time is 1~3 min, and the temperature is 25~40℃; the mixing process further includes removing acetone from the obtained product by rotary evaporation to obtain liquid copper-based hydrotalcite material loaded with avermectin; or removing acetone from the obtained product by rotary evaporation and drying to obtain solid copper-based hydrotalcite material loaded with avermectin.

[0034] This invention also provides the application of the copper-based hydrotalcite material loaded with abamectin as described in the above technical solution or the copper-based hydrotalcite material loaded with abamectin prepared by the preparation method described in the above technical solution in the prevention and control of fungal diseases and pests in crops.

[0035] In this invention, the fungal diseases include one or more of rice blast, root rot, stem rot and clubroot; the insect pests include diamondback moth and / or root-knot nematodes.

[0036] In this invention, the copper-based hydrotalcite material loaded with abamectin material is used in the form of an aqueous dispersion or a solid pesticide.

[0037] In this invention, the copper-based hydrotalcite material loaded with abamectin material aqueous dispersion is applied by spraying or soil application; the concentration of the aqueous dispersion is 0.1~2g / L; and the application rate of the abamectin copper hydrotalcite nano-pesticide is 5~20g / mu.

[0038] To further illustrate the present invention, the copper-based hydrotalcite material loaded with avermectin, its preparation method, and its application are described in detail below with reference to the accompanying drawings and embodiments. However, these descriptions should not be construed as limiting the scope of protection of the present invention.

[0039] Example 1 Weigh out 24.1g of copper nitrate trihydrate, 25.6g of magnesium nitrate hexahydrate and 18.75g of aluminum nitrate nonahydrate and dissolve them in 1000mL of deionized water to prepare solution A; Weigh 24g of sodium hydroxide and 0.576g of SDS and dissolve them in 1000 mL of deionized water to prepare solution B; Solution A and solution B were mixed in a colloid mill and reacted at 5000 r / min for 2 min to obtain SDS-modified copper hydrotalcite nanosheet solution. The SDS-modified copper hydrotalcite nanosheet solution was centrifuged at 6000 rpm for 10 min. The supernatant was removed, and 10 g of the resulting solid hydrotalcite carrier was dispersed in 500 mL of water for later use. Dissolve 5.26 g of avermectin technical grade (95% purity) in 50 mL of acetone. Mix the resulting clarified avermectin with the above-mentioned hydrotalcite carrier dispersion and continue shearing at 3000 r / min for 2 min. After removing the acetone by rotary evaporation, the avermectin copper hydrotalcite nanopesticide mother liquor is obtained.

[0040] Example 2 Weigh 9.64 g of copper nitrate trihydrate and 7.5 g of aluminum nitrate nonahydrate and dissolve them in 200 mL of deionized water to prepare solution A; Weigh 4.8g of sodium hydroxide and 0.0288g of SDS and dissolve them in 200 mL of deionized water to prepare solution B; Solution A and solution B were mixed in a colloid mill and reacted at 6000 r / min for 3 min to obtain an SDS-modified copper hydrotalcite nanosheet solution. The SDS-modified copper hydrotalcite nanosheet solution was centrifuged at 8000 rpm for 10 min. The supernatant was removed, and 10 g of the resulting solid hydrotalcite carrier was dispersed in 500 mL of water for later use. Dissolve 1.05 g of avermectin technical grade (95% purity) in acetone. Mix the resulting clarified avermectin with the above-mentioned hydrotalcite carrier dispersion and continue shearing at 3000 r / min for 3 min. After removing acetone by rotary evaporation, the avermectin copper hydrotalcite nanopesticide mother liquor is obtained.

[0041] Example 3 Weigh out 24.1g of copper nitrate trihydrate, 29.7g of zinc nitrate hexahydrate and 18.75g of aluminum nitrate nonahydrate and dissolve them in 1000mL of deionized water to prepare solution A; Weigh 24g of sodium hydroxide and 0.576g of SDS and dissolve them in 1000 mL of deionized water to prepare solution B; Solution A and solution B were mixed in a colloid mill and reacted at 5000 r / min for 2 min to obtain SDS-modified copper hydrotalcite nanosheet solution. The SDS-modified copper hydrotalcite nanosheet solution was centrifuged at 6000 rpm for 10 min. The supernatant was removed, and 10 g of the resulting solid hydrotalcite carrier was dispersed in 500 mL of water for later use. 2.63 g of avermectin technical grade (95% purity) was dissolved in 100 mL of isopropanol. The resulting clarified avermectin was mixed with the above-mentioned hydrotalcite carrier dispersion and sheared continuously at 3000 r / min for 2 min. After removing acetone by rotary evaporation, the avermectin copper hydrotalcite nanopesticide mother liquor was obtained.

[0042] Comparative Example 1 Weigh 25.6g of magnesium nitrate hexahydrate and 18.75g of aluminum nitrate nonahydrate and dissolve them in 1000mL of deionized water to prepare solution A; Weigh 24g of sodium hydroxide and 0.576g of SDS and dissolve them in 1000 mL of deionized water to prepare solution B; Solution A and solution B were mixed in a colloid mill and reacted at 5000 r / min for 2 min to obtain SDS-modified copper hydrotalcite nanosheet solution. The SDS-modified copper hydrotalcite nanosheet solution was centrifuged at 6000 rpm for 10 min. The supernatant was removed, and 10 g of the resulting solid hydrotalcite carrier was dispersed in 500 mL of water for later use. Dissolve 5.26 g of abamectin technical grade (95% purity) in 50 mL of acetone. Mix the resulting clarified abamectin with the above-mentioned hydrotalcite carrier dispersion and continue shearing at 3000 r / min for 2 min. After removing the acetone by rotary evaporation, the hydrotalcite abamectin nanopesticide mother liquor is obtained.

[0043] Application examples Using rice as the experimental crop, the control efficacy of the abamectin copper-hydrotalcite nanopesticide prepared in Example 1 against rice blast was evaluated. The test method was as follows: Using the rice blast pathogen *Strombus oryzae* as the target, the abamectin copper-hydrotalcite nanopesticide was prepared into an aqueous dispersion with a concentration of 2.0 g / L. After rice seedling transplanting, the rice was irrigated with a culture medium solution containing *Strombus oryzae*, and simultaneously sprayed with the abamectin copper-hydrotalcite nanopesticide. Spraying was performed twice, once every two days. Seven days after application, the control efficacy was compared between the control group sprayed with the abamectin copper-hydrotalcite nanopesticide, the control group sprayed with a commercially available formulation (such as Keshad 3000), and the control group of Example 1 (which did not contain copper-hydrotalcite).

[0044] The results are shown in Table 1. The application of abamectin-copper hydrotalcite nanopesticide effectively controlled the occurrence of rice blast. Copper-based pesticides such as Kocide 3000 were not effective in controlling rice blast, and copper-free hydrotalcite abamectin had almost no effect on rice blast.

[0045] Table 1. Effect of Avermectin Copper Hydrotalcite Nanopesticide on Rice Blast in Example 1

[0046] Application Example 2 Using rice as the experimental crop, the control efficacy of the abamectin-copper-hydrotalcite nanopesticide prepared in Example 2 against the rice stem borer was evaluated. The test method was as follows: rice seedlings were transplanted after germination, and the pesticide was applied twice, with a one-day interval between applications, 7 days later. Twenty-four hours after the two applications, the rice stems were removed and inoculated with 20 second-instar rice stem borers per treatment. The control efficacy of each treatment was calculated after 48 hours. The results are shown in Table 2. The application of the abamectin-copper-hydrotalcite nanopesticide effectively controlled the occurrence of the rice stem borer. The control group, abamectin emulsifiable concentrate (Hebei Xingbai Agricultural Technology Co., Ltd.), showed poor control efficacy due to less accumulation in the stem where the pest mainly occurs and difficulty targeting the pest's burrowing behavior. The copper-free hydrotalcite abamectin also showed control efficacy against the rice stem borer. Therefore, the inclusion of copper is a core factor in achieving the fungal disease control effect of abamectin-copper-hydrotalcite.

[0047] Table 2. Efficacy of Avermectin Copper Hydrotalcite Nanopesticide in Example 2 against Rice Stem Borer

[0048] Application Example 3 Using cucumber as the experimental crop, the control efficacy of the abamectin copper-hydrotalcite nanopesticide prepared in Example 3 against root-knot nematodes was evaluated. The test method was as follows: Abamectin copper-hydrotalcite nanopesticide was prepared into an aqueous dispersion with a concentration of 0.2 g / L and applied as a root drench twice after transplanting cucumber seedlings, with an interval of 24 hours. Root-knot nematodes were inoculated 24 hours after the two root drenches. The control efficacy of the abamectin copper-hydrotalcite nanopesticide and the abamectin emulsifiable concentrate formulation (Hebei Xingbai Agricultural Technology Co., Ltd.) was statistically analyzed on the 7th day after inoculation. The results are shown in Table 3. The application of the abamectin copper-hydrotalcite nanopesticide effectively controlled the occurrence of cucumber root-knot nematodes. However, the control group, the abamectin emulsifiable concentrate formulation, had poor systemic absorption and was easily leached by water, resulting in unsatisfactory control efficacy.

[0049] Table 3. Efficacy of Avermectin Copper Hydrotalcite Nanopesticide against Cucumber Root-Knot Nematodes (Example 3)

[0050] Test case The structure of the avermectin copper hydrotalcite nanopesticide obtained in Example 1 was characterized, and the results are shown below.

[0051] Figure 1 The images show the XRD pattern, infrared spectrum, transmission electron microscope (TEM) image, and elemental analysis spectrum of the abamectin copper hydrotalcite nanopesticide obtained in Example 1 of this invention; where a is the XRD pattern, b is the infrared spectrum, c is the TEM image, and d~h are the elemental spectra of Mg, Cu, Al, and total elements, respectively. Figure 1The results showed that the XRD analysis and infrared spectroscopy of the prepared avermectin copper-hydrotalcite nanopesticide samples exhibited a distinct superposition structure of characteristic peaks from both components, demonstrating the effective loading of avermectin molecules onto the copper-containing hydrotalcite carrier. Transmission electron microscopy (TEM) images and elemental analysis of the nanopesticide structure revealed a plate-like hydrotalcite structure and a uniform distribution of copper, magnesium, and aluminum elements within the structure, further verifying the regular morphology and uniform elemental distribution of the nanopesticide.

[0052] Figure 2 These are transmission electron microscopy (TEM) images of the distribution of the avermectin copper hydrotalcite nanopesticide obtained in Example 1 of this invention within plants and its interaction with the rice blast pathogen. Images a-c show the distribution in the roots, stems, and leaves of cucumber seedlings, respectively; c represents the TEM image of the interaction with the rice blast pathogen; and d represents the TEM image of the interaction with plant cells. Figure 2 The results showed that, through slice observation of cucumber seedlings sprayed with the abamectin copper hydrotalcite nanopesticide prepared in Example 1, the nanopesticide structure was found to be evenly distributed throughout the roots, stems, and leaves of the plant. It could also adsorb onto the surface of the rice blast pathogen, killing it through oxidative destruction of its cell structure. However, the nanopesticide structure adsorbed onto the surface of crop cells had no significant effect on normal cells.

[0053] Figure 3 This image shows the pH-responsive reactive oxygen species (EPS) generation spectrum of the avermectin copper hydrotalcite nanopesticide obtained in Example 1 of this invention, along with XRD, XPS, transmission electron microscopy, and AFM images before and after the response. In the image, a represents oxygen vacancy detection at different pH levels; B represents hydroxyl radical detection at different pH levels; c represents the XRD spectrum; d represents the XPS spectrum at pH 7.0; e represents the XPS spectrum at pH 5.0; f represents the HRTEM image at pH 7.0; g represents the HRTEM image at pH 5.0; h represents the AFM image at pH 7.0; and i represents the AFM image at pH 5.0. Figure 3 The results show that EPR tests under two buffer solution conditions (pH=5.0 and 7.0) demonstrate that avermectin copper hydrotalcite nanopesticides can rapidly generate oxygen vacancies and hydroxyl radicals at pH=5.0, achieving pH-responsive reactive oxygen species release. Further characterization of the nanopesticide structure under pH=5.0 and 7.0 conditions revealed that the nanopesticides treated at pH=5.0 transformed from a hydrotalcite structure to an amorphous state via the breaking of O-Cu-O bonds in the hydrotalcite structure. This process introduces defects into the originally regular crystal lattice structure, generating a large number of oxygen vacancies and promoting the generation of hydroxyl radicals, thus achieving the construction of pH-responsive nanopesticides based on structural transformation.

[0054] Figure 4This is a comparison chart showing the abamectin content in plants 24 hours after application of the abamectin copper hydrotalcite nanopesticide obtained in Example 1 of this invention and a commercially available abamectin emulsifiable concentrate formulation. Figure 4 The results show that abamectin can be efficiently absorbed and utilized by plants through transport mediated by hydrotalcite carriers, which has significant advantages over traditional emulsifiable concentrate formulations.

[0055] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A copper-based hydrotalcite material loaded with avermectin, characterized in that, Includes a copper-based hydrotalcite carrier and avermectin loaded on the surface and pores of the copper-based hydrotalcite carrier; The copper-based hydrotalcite carrier is a surfactant-modified copper-based hydrotalcite carrier; The chemical composition of the copper-based hydrotalcite carrier is Cu. a (M1) b (M2)1(OH)2Y c A d ·mH2O; Where M1 is Mg 2+ Ca 2+ Zn 2+ and Ni 2+ One or more of them; M2 is Fe 3+ Cr 3+ And Al 3+ One or more of the following; Y is a surfactant; A is NO3. - SO4 2- and Cl - One or more of them.

2. The copper-based hydrotalcite material loaded with abamectin according to claim 1, characterized in that, a, b, c, d, and m represent the molar percentages of Cu, M1, M2, Y, and A in the avermectin-loaded material per mole of copper-based hydrotalcite material, respectively; a = 1~4; b = 0~4; c = 0.0001~0.001; d = 1~20; a+b = 2~4; m = 3~20; the chemical composition of the copper-based hydrotalcite carrier maintains charge conservation.

3. The copper-based hydrotalcite material loaded with abamectin according to claim 1, characterized in that, The surfactant includes one or more of sodium dodecyl sulfate, hexadecyltrimethylammonium bromide, and sodium stearate.

4. The copper-based hydrotalcite material loaded with abamectin according to claim 1, characterized in that, The mass of the avermectin is 5-100% of the mass of the copper-based hydrotalcite carrier.

5. The copper-based hydrotalcite material loaded with abamectin according to claim 1, characterized in that, The copper-based hydrotalcite material loaded with avermectin has a particle size of 20~300nm.

6. The method for preparing the copper-based hydrotalcite material loaded with avermectin according to any one of claims 1 to 5, characterized in that, Includes the following steps: A soluble copper salt, a soluble M1 metal salt, a soluble M2 metal salt, an alkaline reagent, and a surfactant were mixed and subjected to a co-precipitation reaction to obtain a copper-based hydrotalcite carrier. Abamectin was loaded onto the copper-based hydrotalcite carrier in the liquid phase to obtain the copper-based hydrotalcite material loaded with abamectin.

7. The preparation method according to claim 6, characterized in that, The coprecipitation reaction is carried out under shear conditions; the shear rate is 3000~5000 r / min, the time is 1~3 min, and the temperature is 25~40℃.

8. The preparation method according to claim 6, characterized in that, The liquid phase loading is carried out under shear conditions, wherein the shear rate is 3000~5000 r / min, the time is 1~3 min, and the temperature is 25~40℃.

9. The application of the copper-based hydrotalcite material loaded with abamectin according to any one of claims 1 to 5 or the copper-based hydrotalcite material loaded with abamectin prepared by the preparation method according to any one of claims 6 to 8 in the prevention and control of fungal diseases or pests in crops.

10. The application according to claim 9, characterized in that, The fungal diseases include one or more of rice blast, root rot, stem rot, and clubroot; the insect pests include diamondback moth and / or root-knot nematodes.

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