A composition with anti-aging, wrinkle-erasing and soothing efficacy and uses thereof

Abstract

The present application provides a kind of composition with anti-aging and wrinkle removal and soothing effect and its application, the composition includes retinol, hydroxypropyl tetrahydropyran triol, palmitoyl pentapeptide-4, acetyl hexapeptide-8, blue copper peptide, ceramide, gynura extract and other core active ingredients, by specific weight portion compounding.The preparation method adopts step emulsification process: first, construct lipid repair phase and soothing gel base, then step emulsification, finally low temperature introduction of liposome-encapsulated retinol and other heat-sensitive active substances, effectively protect the activity of components;The composition is synergized by multiple targets, significantly promotes collagen synthesis, inhibits matrix metalloproteinase activity, reduces inflammatory factor expression, effectively anti-aging while significantly improving skin tolerance, suitable for improving skin aging, reducing irritation and strengthening barrier function, can be widely applied in essence, cream and other cosmetics.

Description

Technical Field

[0001] This invention relates to the field of cosmetic preparation technology, and in particular to a composition having anti-aging, wrinkle-reducing and soothing effects and its application. Background Technology

[0002] With the aging population and rising consumption levels, anti-aging and skin health care have become core demands in the global cosmetics market. Skin aging is a complex physiological process involving multiple stages, including extracellular matrix (ECM) degradation, decreased fibroblast activity, accumulated oxidative stress, and chronic inflammatory responses. Studies have shown that external factors such as UV radiation, environmental pollution, and life stress can accelerate collagen degradation by activating the matrix metalloproteinase (MMP) family, while inhibiting the synthesis of type I collagen (Col-I), leading to reduced skin elasticity and wrinkle formation. Furthermore, chronic inflammatory responses triggered by impaired skin barrier function further exacerbate the skin aging process, creating a vicious cycle of inflammation and aging, manifesting as itchy, wrinkled skin.

[0003] Currently, most anti-aging products on the market focus on a single mechanism, resulting in limited efficacy or irritation. For example, while retinol can promote cell renewal, it can easily cause skin redness and peeling; hydroxypropyl tetrahydropyranotriol (Pro-Xylane) can stimulate glycosaminoglycan synthesis, but its anti-wrinkle effect is limited when used alone. At the same time, traditional soothing ingredients often work through a single anti-inflammatory pathway, making it difficult to address complex imbalances in the skin's microbiome.

[0004] This invention aims to resolve the technical contradiction between the difficulty in achieving both high-efficiency anti-aging ingredients and skin tolerance, and provides a composition that can maintain or even enhance anti-aging efficiency while providing immediate relief from irritation and long-term active repair of the skin barrier, thus making it suitable for a wider range of people, including those with sensitive skin. Summary of the Invention

[0005] In view of this, the present invention proposes a composition with anti-aging, wrinkle-reducing and soothing effects and its application, thereby solving the above-mentioned problems.

[0006] The technical solution of this invention is achieved as follows: a composition with anti-aging, wrinkle-reducing, and soothing effects, comprising the following raw materials in parts by weight: retinol 0.1-2 parts, hydroxypropyl tetrahydropyrantriol 3-12 parts, rhamnose 2-8 parts, palmitoyl pentapeptide-4 0.5-3 parts, acetyl hexapeptide-8 0.3-2 parts, blue copper peptide 0.1-1 parts, vitamin E 0.5-4 parts, ergothioneine 0.2-1.5 parts, astaxanthin 0.01-0.5 parts, ceramide 1-5 parts, cholesterol 0.5-2 parts, dipotassium glycyrrhizate 0.3-2 parts, allantoin 0.2-1.8 parts, panthenol 1-6 parts, centella asiatica extract 2-8 parts, tribulus terrestris extract 0.1-0.3 parts, hyaluronic acid 0.5-3 parts, phytosphingosine 0.05-0.5 parts, and soybean lecithin 10-16 parts.

[0007] Preferably, the ceramide is ceramide NP and ceramide AP in a mass ratio of 3-5:1; the hyaluronic acid is compounded from 2000-5000kDa, 500-1000kDa and 10-50kDa in a weight ratio of (1-3):(2-4):5.

[0008] Preferably, the extraction method of the Centella asiatica extract includes the following steps: A1. Mix the dried snow grassland material with a 30-70% v / v ethanol aqueous solution at a weight ratio of 1:10-30; A2. Extract with ultrasound assistance at 40-60℃ 1-3 times, with a power of 200-500W and a frequency of 20-40kHz, for 10-40 minutes each time; A3. Combine the extracts, filter, and concentrate under reduced pressure until there is no alcohol odor to obtain the concentrated solution; A4. The concentrated solution is enriched and purified by passing it through a macroporous adsorption resin column. Impurities are removed by elution with 10-30% v / v ethanol. After elution, the eluent is collected, concentrated, and dried to obtain the Centella asiatica extract.

[0009] Preferably, the extraction method of the Tribulus terrestris extract includes the following steps: B1. Crush the Tribulus terrestris fruit through a 20-40 mesh sieve, and use a 50-70% v / v ethanol solution to perform ultrasonic-assisted extraction 1-3 times at 60-80℃. Combine the extracts. B2. Centrifuge the extract at 3000-5000 rpm for 10-20 minutes, collect the supernatant, filter, and obtain a clear extract; B3. The clarified extract is concentrated under reduced pressure at 40-50℃ and a vacuum of 0.06-0.09MPa until no alcohol odor is detected, and the concentrate is then freeze-dried at -40℃ to -50℃ and a vacuum of ≤10Pa. After pulverizing, the concentrate is passed through an 80-mesh sieve to obtain the Tribulus terrestris extract.

[0010] More preferably, in step B1, the mass-to-volume ratio of the Tribulus terrestris fruit to the ethanol solution is 2-8 g / 50 mL, the extraction time is 30-90 minutes each time, the ultrasonic power is 100-300 W, and the ultrasonic frequency is 20-60 kHz.

[0011] The present invention also provides a method for preparing the composition, comprising the following steps: S1. Constructing the repair phase: Ceramide, cholesterol, and phytosphingosine are melt-blended at 60-70℃ to form a lipid mixture; S2. Preparation of soothing gel: At 25-35℃, Centella asiatica extract, dipotassium glycyrrhizate, allantoin, panthenol, rhamnose, Tribulus terrestris extract, and hyaluronic acid are dispersed in water, and a high molecular weight polymer is added and stirred to swell, forming a gel base. S3. Stepwise emulsification: The lipid mixture obtained in step S1 is slowly added to the gel base obtained in step S2 under stirring, and initial emulsification is carried out at a shear rate of 5000-8000 rpm at 45-55℃; then the temperature is cooled to ≤40℃, acetyl hexapeptide-8 and palmitoyl pentapeptide-4 are added, and the mixture is stirred evenly. S4. Low-temperature introduction of active ingredients: In the emulsion obtained in step (3), retinol, hydroxypropyl tetrahydropyrantriol, copper peptide, ergothionein and astaxanthin are added sequentially at a temperature below 30°C and stirred at low speed until homogeneous to obtain the composition.

[0012] Preferably, the polymer in step S2 is at least one of carbomer, xanthan gum, and sodium polyglutamate, and the amount of the polymer added is 0.05-1.0% of the total weight of the composition.

[0013] Preferably, in step S4, the retinol encapsulated in the liposomes is prepared by the following steps: C1. Membrane material pretreatment: Weigh vitamin E and soybean lecithin and add 1-3 times the volume of chloroform-methanol mixed solvent (volume ratio 2:1). Stir at 30-35℃ for 20-30 minutes until completely dissolved to obtain membrane material solution. C2. Film formation: The membrane solution is rotary evaporated at 35-40℃ and a vacuum of 0.06-0.08MPa for 30-45 minutes to remove organic solvents and form a uniform lipid film on the inner wall. C3. Hydration and dispersion: Dissolve retinol in anhydrous ethanol at a weight ratio of 1:5-8, add it to 3-5 times the total weight of the membrane material in deionized water, stir evenly, and pour it into the flask containing the lipid membrane. Hydrate by stirring at a constant temperature of 40-45℃ for 60-90 minutes to obtain a crude liposome suspension. C4. Homogenization and Refinement: The crude liposome suspension is successively dispersed by ultrasonication at 200-300W for 15-20 min and homogenized by high pressure at 20-25MPa 3-4 times to control the liposome particle size to 50-200nm. After purification and freeze-drying, retinol powder encapsulated in liposomes is obtained.

[0014] The composition of the present invention, which has anti-aging, wrinkle-reducing, and soothing effects, is used in the preparation of cosmetics for improving skin aging, reducing skin irritation, and strengthening the skin barrier function.

[0015] The cosmetics of this invention are serums, lotions, face creams, eye creams, or ampoule serums.

[0016] Compared with the prior art, the beneficial effects of the present invention are: The composition of this invention can improve skin aging associated with neurogenic inflammation. Through the synergistic effect of retinol, hydroxypropyltetrahydropyranotriol, and complex peptides, it significantly increases the synthesis of Col-I and inhibits MMP-1 activity, thereby achieving the reconstruction of the dermal collagen network. The complex system of dipotassium glycyrrhizate, Centella asiatica extract, and ceramide can reduce the expression of pro-inflammatory factors such as IL-6 and TNF-α, while mitigating the inflammatory cascade reaction by regulating NO release. The ratio of cholesterol, phytosphingosine, and ceramide promotes keratinocyte migration. Combined with liposome encapsulation technology, it reduces the irritation of active ingredients such as retinol, achieving a balance between efficacy and gentleness. Ergothioneine and astaxanthin form an antioxidant synergy, effectively scavenging free radicals and reducing oxidative stress damage to skin fibroblasts. This invention first constructs a lipid repair phase, then forms a soothing gel base, followed by stepwise emulsification, and finally introduces heat-sensitive active ingredients such as retinol encapsulated in liposomes at low temperature. The soothing ingredients are released first and rapidly from the gel network, acting on the skin immediately to relieve potential irritation, achieving a soothing base before anti-aging. This process effectively protects the activity of the ingredients and constructs a formulation structure conducive to functional achievement. This composition can be used to prepare cosmetics that improve skin aging, reduce irritation, and strengthen the skin barrier function. Detailed Implementation

[0017] To better understand the technical content of this invention, specific embodiments are provided below to further illustrate the invention.

[0018] Unless otherwise specified, the experimental methods used in the embodiments of this invention are all conventional methods.

[0019] Unless otherwise specified, all materials and reagents used in the embodiments of this invention are commercially available.

[0020] Example 1 A composition with anti-aging, wrinkle-reducing, and soothing effects comprises the following ingredients in parts by weight: 0.1 parts retinol, 3 parts hydroxypropyl tetrahydropyrantriol, 2 parts rhamnose, 0.5 parts palmitoyl pentapeptide-4, 0.3 parts acetyl hexapeptide-8, 0.1 parts copper peptide, 0.5 parts vitamin E, 0.2 parts ergothioneine, 0.01 parts astaxanthin, 1 part ceramide, 0.5 parts cholesterol, 0.3 parts dipotassium glycyrrhizate, 0.2 parts allantoin, 1 part panthenol, 2 parts centella asiatica extract, 0.1 parts tribulus terrestris extract, 0.5 parts hyaluronic acid, 0.05 parts phytosphingosine, and 10 parts soybean lecithin; The ceramide is ceramide NP and ceramide AP in a mass ratio of 3:1; The hyaluronic acid is composed of molecules with molecular weights of 2000-5000kDa, 500-1000kDa, and 10-50kDa in a weight ratio of 1:2:5.

[0021] Example 2 A composition with anti-aging, wrinkle-reducing, and soothing effects comprises the following ingredients in parts by weight: 2 parts retinol, 12 parts hydroxypropyl tetrahydropyrantriol, 8 parts rhamnose, 3 parts palmitoyl pentapeptide-4, 2 parts acetyl hexapeptide-8, 1 part copper peptide, 4 parts vitamin E, 1.5 parts ergothioneine, 0.5 parts astaxanthin, 5 parts ceramide, 2 parts cholesterol, 2 parts dipotassium glycyrrhizate, 1.8 parts allantoin, 6 parts panthenol, 8 parts centella asiatica extract, 0.3 parts tribulus terrestris extract, 3 parts hyaluronic acid, 0.5 parts phytosphingosine, and 16 parts soybean lecithin; The ceramide is ceramide NP and ceramide AP in a mass ratio of 5:1; The hyaluronic acid is composed of molecules with molecular weights of 2000-5000kDa, 500-1000kDa, and 10-50kDa in a weight ratio of 3:4:5.

[0022] Example 3 A composition with anti-aging, wrinkle-reducing, and soothing effects comprises the following ingredients in parts by weight: 0.5 parts retinol, 8 parts hydroxypropyl tetrahydropyrantriol, 5 parts rhamnose, 2 parts palmitoyl pentapeptide-4, 1.5 parts acetyl hexapeptide-8, 0.5 parts copper peptide, 2 parts vitamin E, 0.9 parts ergothioneine, 0.3 parts astaxanthin, 3 parts ceramide, 1.5 parts cholesterol, 1.5 parts dipotassium glycyrrhizate, 1.2 parts allantoin, 3 parts panthenol, 5 parts centella asiatica extract, 0.2 parts tribulus terrestris extract, 2 parts hyaluronic acid, 0.2 parts phytosphingosine, and 12 parts soybean lecithin; The ceramide is ceramide NP and ceramide AP in a mass ratio of 4:1; The hyaluronic acid is composed of molecules with molecular weights of 2000-5000kDa, 500-1000kDa, and 10-50kDa in a weight ratio of 2:3:5.

[0023] The above Examples 1-3 were prepared using the following methods: 1. The extraction method of Centella asiatica extract includes the following steps: A1. Mix the dried snow grassland material with a 50% v / v ethanol aqueous solution at a weight ratio of 1:20; A2. Extract twice with ultrasound at 50℃, with a power of 300W and a frequency of 30kHz, for 30 minutes each time; A3. Combine the extracts, filter, and concentrate under reduced pressure until there is no alcohol odor to obtain the concentrated solution; A4. The concentrated solution is enriched and purified by passing it through an HPD300 macroporous adsorption resin column. Impurities are removed by elution with 20% v / v ethanol. After elution, the eluent is collected, concentrated, and dried to obtain the Centella asiatica extract.

[0024] 2. The extraction method of Tribulus terrestris extract includes the following steps: B1. Crush the Tribulus terrestris fruit through a 30-mesh sieve. Use a 60% v / v ethanol solution with a mass-to-volume ratio of 5g / 50mL for ultrasonic-assisted extraction twice at 70℃, each extraction lasting 60 minutes. The ultrasonic power is 200W and the ultrasonic frequency is 40kHz. Combine the extracts. B2. Centrifuge the extract at 4000 rpm for 15 minutes, collect the supernatant, filter, and obtain a clear extract; B3. The clarified extract is concentrated under reduced pressure at 45°C and 0.07MPa until no alcohol odor is detected, and the concentrate is then freeze-dried at -45°C and ≤10Pa. After pulverization, it is passed through an 80-mesh sieve to obtain the Tribulus terrestris extract.

[0025] 3. Liposome-encapsulated retinol is prepared via the following steps: C1. Membrane material pretreatment: Weigh vitamin E and soybean lecithin and add them to 2 times the volume of chloroform-methanol mixed solvent (volume ratio 2:1). Stir at 32℃ for 25 minutes until completely dissolved to obtain membrane material solution. C2. Film formation: The membrane solution is rotary evaporated at 38°C and 0.07MPa for 40 minutes to remove organic solvents and form a uniform lipid film on the inner wall. C3. Hydration and dispersion: Dissolve retinol in anhydrous ethanol at a weight ratio of 1:7, add it to 4 times the total weight of the membrane material in deionized water, stir evenly, and pour it into the flask containing the lipid membrane. Hydrate by stirring at 42°C for 70 minutes to obtain a crude liposome suspension. C4. Homogenization and Refinement: The crude liposome suspension was successively dispersed by ultrasonication at 250W for 18 min and homogenized by high pressure at 22MPa three times to control the liposome particle size to 100nm. After purification and freeze-drying, retinol powder encapsulated in liposomes was obtained.

[0026] Preparation method of a composition with anti-aging, wrinkle-reducing, and soothing effects: S1. Constructing the repair phase: Ceramide, cholesterol, and phytosphingosine are melt-blended at 65°C to form a lipid mixture; S2. Preparation of soothing gel: At 30°C, Centella asiatica extract, dipotassium glycyrrhizate, allantoin, panthenol, rhamnose, Tribulus terrestris extract, and hyaluronic acid are dispersed in water, and carbomer is added and stirred to swell. The amount added is 0.08% of the total weight of the composition to form a gel base. S3. Stepwise emulsification: The lipid mixture obtained in step S1 is slowly added to the gel base obtained in step S2 under stirring, and initial emulsification is carried out at 50°C and a shear rate of 7000 rpm; then the temperature is cooled to ≤40°C, acetyl hexapeptide-8 and palmitoyl pentapeptide-4 are added, and the mixture is stirred evenly. S4. Low-temperature introduction of active ingredients: In the emulsion obtained in step (3), retinol, hydroxypropyl tetrahydropyrantriol, copper peptide, ergothionein, and astaxanthin, which are pre-encapsulated with liposomes, are added sequentially at a temperature below 30°C. The mixture is stirred at a low speed of 300 rpm until homogeneous to obtain the composition.

[0027] Comparative Example 1 The difference between this comparative example and Example 3 is that it does not contain ceramide, cholesterol, and phytosphingosine, and step S1 is omitted. The other raw materials and preparation methods are the same.

[0028] Comparative Example 2 The difference between this comparative example and Example 3 is that it does not contain Centella asiatica extract and dipotassium glycyrrhizate, while the other raw materials and preparation methods are the same.

[0029] Comparative Example 3 The difference between this comparative example and Example 3 is that it does not contain panthenol or tribulus extract, while the other raw materials and preparation methods are the same.

[0030] Comparative Example 4 The difference between this comparative example and Example 3 is that the ceramide used is only ceramide NP, while the other raw materials and preparation methods are the same.

[0031] Comparative Example 5 The difference between this comparative example and Example 3 is that liposomes were not used to encapsulate retinol; the other raw materials and preparation methods are the same.

[0032] Comparative Example 6 The difference between this comparative example and Example 3 is that palmitoyl pentapeptide-4, acetyl hexapeptide-8, and blue copper peptide were not added; the other raw materials and preparation methods are the same.

[0033] Comparative Example 7 The difference between this comparative example and Example 3 is that it uses a conventional one-step emulsification method. All oil phase components and aqueous phase components are mixed at once: Oil phase preparation: Ceramide, cholesterol, phytosphoprotein, liposome-encapsulated retinol, vitamin E, and soybean lecithin are mixed, heated to 80°C, and stirred until completely dissolved and homogeneous.

[0034] Aqueous phase preparation: Hydroxypropyl tetrahydropyrantriol, Centella asiatica extract, dipotassium glycyrrhizate, allantoin, panthenol, rhamnose, Tribulus terrestris extract, hyaluronic acid, and ergothioneine were dissolved in water and heated to 80°C, and stirred until completely dissolved or uniformly dispersed.

[0035] Emulsification: Under stirring, the oil phase is slowly poured into the aqueous phase, and then homogenized and emulsified for 8 minutes at 10,000 rpm using a high-shear disperser at 80°C to form a preliminary emulsion.

[0036] Cooling and subsequent addition: Stir the above emulsion to cool it down. When the temperature drops to about 40°C, add astaxanthin and peptides, and continue stirring and cooling to room temperature.

[0037] Performance testing To verify the anti-aging, wrinkle-reducing, and soothing effects of the compositions of the present invention, the following performance tests were conducted on Examples 1-3 and Comparative Examples 1-6 using in vitro testing methods: I. Anti-aging efficacy test 1.1 Cell Model Construction: Human dermal fibroblasts (HDF) were cultured in DMEM medium containing 10% fetal bovine serum at 37°C in a 5% CO2 incubator until the logarithmic growth phase, and then seeded into 96-well plates (5 × 10⁶ cells / wells). 3 (1 cell / well), cultured for 24 hours and then used for testing.

[0038] 1.2 Sample preparation: The compositions of Examples 1-3 and Comparative Examples 1-7 were diluted with serum-free DMEM medium to concentrations of 0.1 mg / mL, 0.5 mg / mL and 1.0 mg / mL, respectively, and added to cell wells. Three replicates were set up for each group. A blank control group (only culture medium was added) and a model control group (H2O2-induced oxidative damage) were also set up.

[0039] 1.3 Test Indicators and Methods: (1) Cell viability detection: The CCK-8 method was used. After culturing for 48 hours, 10 μL of CCK-8 solution was added to each well and cultured for another 2 hours. The absorbance (OD value) was measured at 450 nm using an ELISA reader, and cell viability was calculated.

[0040] (2) Determination of collagen synthesis: The content of type I collagen (Col-I) in cell supernatant was detected by ELISA, and the operation was strictly carried out in accordance with the kit instructions.

[0041] (3) MMP-1 inhibition rate detection: The level of matrix metalloproteinase-1 (MMP-1) was detected by ELISA and the inhibition rate was calculated.

[0042] 1.4 The test results are as follows (concentration of 1.0 mg / mL): Table 1. Anti-aging efficacy test results of Examples 1-3 and Comparative Examples 1-7 (x±s, n=3)

[0043] Results: As shown in Table 1, compared with the model control group, Examples 1-3 and Comparative Examples 1-6 all improved cell viability, promoted Col-I synthesis, and inhibited MMP-1 activity to varying degrees. The overall effect of the Example groups was significantly better than that of the Comparative Examples (P < 0.05). Example 3 showed the best overall anti-aging effect, with cell viability of 95.3 ± 2.4%, Col-I content of 256.4 ± 8.2 ng / mL, and MMP-1 inhibition rate of 58.9 ± 3.5%, representing increases of 62.6%, 310.2%, and 58.9% respectively compared to the model control group, and significantly higher than Examples 1-2 and all Comparative Examples (P < 0.05).

[0044] The indicators of Comparative Examples 1 and 4 were significantly lower than those of the Example, demonstrating that the synergistic effect of ceramides, cholesterol, and phytosphingosine, and the specific ratio of ceramide complex (NP and AP), contributes to building a complete skin barrier and thus synergistically enhancing anti-aging effects.

[0045] The effects of Comparative Examples 2 and 3 were inferior to those of the Example, indicating that the soothing system composed of Centella asiatica extract, dipotassium glycyrrhizate, etc., can not only relieve irritation, but its anti-inflammatory effect also plays a positive role in maintaining skin homeostasis and assisting in anti-aging.

[0046] Comparative Examples 5 and 6 demonstrate that liposome-encapsulated retinol can protect retinol activity, reduce its irritation, and thus exert a highly effective anti-aging effect. Synthetic peptides and natural extracts, among other raw materials, work synergistically to promote collagen production, repair the dermal matrix, and inhibit matrix metalloproteinase-1 (MMP-1), effectively reducing internal skin activity and protecting anti-aging results.

[0047] The data from Comparative Example 7 show that the stepwise emulsification and low-temperature introduction of active ingredients used in this invention can maximize the protection of the efficacy of various active ingredients, especially peptides and retinol, which is the core guarantee for achieving excellent anti-aging effects.

[0048] II. Evaluation of Soothing / Anti-inflammatory Efficacy 2.1 Cell model construction: Human immortalized keratinocytes (HaCaT) were cultured in DMEM / F12 medium containing 10% fetal bovine serum and seeded in 24-well plates (2×10⁶ cells / wells). 4 (1 cell / well), after culturing for 24 h, an inflammation model was established by inducing with 1 μg / mL lipopolysaccharide (LPS) for 24 h.

[0049] 2.2 Sample processing: The compositions were diluted with culture medium to 0.2 mg / mL, 0.5 mg / mL and 1.0 mg / mL, and added to the inflammatory model cells. Each group was divided into 3 replicates. A blank control group (uninduced) and a model control group (LPS induced only) were also set up.

[0050] 2.3 Test Indicators and Methods: (1) Detection of inflammatory factors: The relative expression levels of IL-6 and TNF-α mRNA in cells were detected by RT-PCR, with GAPDH as the internal reference gene.

[0051] (2) NO release determination: The content of nitric oxide (NO) in cell supernatant was detected by the Griess reagent method, and the OD value was measured at 540 nm.

[0052] (3) Cell migration ability assessment: The scratch test was used. After scratching the cell monolayer, the sample was added and photographed at 0h and 24h respectively, and the scratch healing rate was calculated.

[0053] 2.4 Data statistics: The data are expressed as mean ± standard deviation (x ± s). The t-test was used for comparisons between groups. P < 0.05 was considered statistically significant.

[0054] 2.5 The test results are shown in the table below (1.0 mg / mL concentration): Table 2. Results of soothing / anti-inflammatory efficacy tests in Examples 1-3 and Comparative Examples 1-7 (x±s, n=3)

[0055] Results: As shown in Table 2, compared with the model control group, Examples 1-3 and Comparative Examples 1-6 all reduced the relative expression levels of IL-6 and TNF-α mRNA and NO content to varying degrees, and improved the scratch healing rate. The overall effect of the Example groups was significantly better than that of the Comparative Examples (P < 0.05). Among them, Example 3 showed the best comprehensive soothing and anti-inflammatory effect, with a relative expression level of IL-6 mRNA of 1.32 ± 0.09, a relative expression level of TNF-α mRNA of 1.21 ± 0.08, a NO content of 18.7 ± 1.6 μmol / L, and a scratch healing rate of 65.4 ± 3.8%, which was significantly higher than that of Examples 1-2 and all Comparative Examples (P < 0.05).

[0056] The inflammatory factor levels in Comparative Example 1 were significantly higher than those in Example 3, with a scratch healing rate of only 42.6 ± 2.5%, indicating that the skin barrier repair system is crucial for inhibiting the inflammatory cascade and promoting barrier regeneration. The anti-inflammatory effects of Comparative Examples 2 and 3 were significantly weakened, demonstrating that the multi-component soothing ingredients achieve synergistic anti-inflammatory repair by inhibiting the release of pro-inflammatory factors and enhancing the migration ability of keratinocytes.

[0057] Although the inflammatory markers in Comparative Examples 5 and 6 were better than those in the model control group, they were weaker than those in Example 3, suggesting that liposome encapsulation technology can reduce the irritation of the active ingredients, while components such as copper peptides assist in soothing by regulating the cytokine network. The NO content and scratch healing rate in Comparative Example 7 were significantly worse than those in Example 3, further verifying the key role of stepwise emulsification and low-temperature processing in preserving the stability of the soothing active ingredients.

[0058] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A composition having anti-aging, wrinkle-reducing, and soothing effects, characterized in that, The product contains the following ingredients in parts by weight: retinol 0.1-2 parts, hydroxypropyl tetrahydropyrantriol 3-12 parts, rhamnose 2-8 parts, palmitoyl pentapeptide-4 0.5-3 parts, acetyl hexapeptide-8 0.3-2 parts, blue copper peptide 0.1-1 parts, vitamin E 0.5-4 parts, ergothioneine 0.2-1.5 parts, astaxanthin 0.01-0.5 parts, ceramide 1-5 parts, cholesterol 0.5-2 parts, dimethyl glycyrrhizate 0.3-2 parts, allantoin 0.2-1.8 parts, panthenol 1-6 parts, centella asiatica extract 2-8 parts, tribulus terrestris extract 0.1-0.3 parts, hyaluronic acid 0.5-3 parts, phytosphingosine 0.05-0.5 parts, and soybean lecithin 10-16 parts.

2. The composition according to claim 1, characterized in that, The ceramide is ceramide NP and ceramide AP in a mass ratio of 3-5:1; the hyaluronic acid is a compound of 2000-5000kDa, 500-1000kDa and 10-50kDa in a weight ratio of (1-3):(2-4):

5.

3. The composition according to claim 1, characterized in that, The extraction method of the Centella asiatica extract includes the following steps: A1. Mix the dried snow grassland material with a 30-70% v / v ethanol aqueous solution at a weight ratio of 1:10-30; A2. Extract with ultrasound assistance at 40-60℃ 1-3 times, with a power of 200-500W and a frequency of 20-40kHz, for 10-40 minutes each time; A3. Combine the extracts, filter, and concentrate under reduced pressure until there is no alcohol odor to obtain the concentrated solution; A4. The concentrated solution is enriched and purified by passing it through a macroporous adsorption resin column. Impurities are removed by elution with 10-30% v / v ethanol. After elution, the eluent is collected, concentrated, and dried to obtain the Centella asiatica extract.

4. The composition according to claim 1, characterized in that, The extraction method of the Tribulus terrestris extract includes the following steps: B1. Crush the Tribulus terrestris fruit through a 20-40 mesh sieve, and use a 50-70% v / v ethanol solution to perform ultrasonic-assisted extraction 1-3 times at 60-80℃. Combine the extracts. B2. Centrifuge the extract at 3000-5000 rpm for 10-20 minutes, collect the supernatant, filter, and obtain a clear extract; B3. The clarified extract is concentrated under reduced pressure at 40-50℃ and a vacuum of 0.06-0.09MPa until no alcohol odor is detected, and the concentrate is then freeze-dried at -40℃ to -50℃ and a vacuum of ≤10Pa. After pulverizing, the concentrate is passed through an 80-mesh sieve to obtain the Tribulus terrestris extract.

5. The composition according to claim 4, characterized in that, In step B1, the mass-to-volume ratio of the Tribulus terrestris fruit to the ethanol solution is 2-8 g / 50 mL, the extraction time is 30-90 minutes each time, the ultrasonic power is 100-300 W, and the ultrasonic frequency is 20-60 kHz.

6. A method for preparing the composition according to any one of claims 1-5, characterized in that, Includes the following steps: S1. Constructing the repair phase: Ceramide, cholesterol, and phytosphingosine are melt-blended at 60-70℃ to form a lipid mixture; S2. Preparation of soothing gel: At 25-35℃, Centella asiatica extract, dipotassium glycyrrhizate, allantoin, panthenol, rhamnose, Tribulus terrestris extract, and hyaluronic acid are dispersed in water, and a high molecular weight polymer is added and stirred to swell, forming a gel base. S3. Stepwise emulsification: The lipid mixture obtained in step S1 is slowly added to the gel base obtained in step S2 under stirring, and initial emulsification is carried out at a shear rate of 5000-8000 rpm at 45-55℃; then the temperature is cooled to ≤40℃, acetyl hexapeptide-8 and palmitoyl pentapeptide-4 are added, and the mixture is stirred evenly. S4. Low-temperature introduction of active ingredients: In the emulsion obtained in step (3), retinol, hydroxypropyl tetrahydropyrantriol, copper peptide, ergothionein and astaxanthin are added sequentially at a temperature below 30°C and stirred at low speed until homogeneous to obtain the composition.

7. The preparation method according to claim 1, characterized in that, In step S2, the polymer is at least one of carbomer, xanthan gum, and sodium polyglutamate, and the amount of the polymer added is 0.05-1.0% of the total weight of the composition.

8. The preparation method according to claim 1, characterized in that, In step S4, the retinol encapsulated in the liposomes is prepared through the following steps: C1. Membrane material pretreatment: Weigh vitamin E and soybean lecithin and add them to 1-3 times the volume of chloroform-methanol mixed solvent. Stir at 30-35℃ for 20-30 minutes until completely dissolved to obtain membrane material solution. C2. Film formation: The membrane solution is rotary evaporated at 35-40℃ and a vacuum of 0.06-0.08MPa for 30-45 minutes to remove organic solvents and form a uniform lipid film on the inner wall. C3. Hydration and dispersion: Dissolve retinol in anhydrous ethanol at a weight ratio of 1:5-8, add it to 3-5 times the total weight of the membrane material in deionized water, stir evenly, and pour it into the flask containing the lipid membrane. Hydrate by stirring at a constant temperature of 40-45℃ for 60-90 minutes to obtain a crude liposome suspension. C4. Homogenization and Refinement: The crude liposome suspension is successively dispersed by ultrasonication at 200-300W for 15-20 min and homogenized by high pressure at 20-25MPa 3-4 times to control the liposome particle size to 50-200nm. After purification and freeze-drying, retinol powder encapsulated in liposomes is obtained.

9. The use of the composition with anti-aging, wrinkle-reducing, and soothing effects according to any one of claims 1-5 in the preparation of cosmetics for improving skin aging, reducing skin irritation, and strengthening the skin barrier function.

10. A cosmetic product, characterized in that, The cosmetic comprises the multi-effect anti-aging and soothing composition according to any one of claims 1-5, wherein the cosmetic is an essence, lotion, face cream, eye cream or ampoule essence.

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