A melanotrophic composition and method of making and using same

Abstract

The application discloses a melanin promoting composition and a preparation method and application thereof, and belongs to the technical field of cosmetics and biological agents. The melanin promoting composition is composed of the following components in percentage by mass: acetyl hexapeptide-1 0.01-0.5 parts, Poria cocos extract 1-5 parts, sophorolipid 0.5-5 parts, 1,3-dihydroxyacetone 3-20 parts, a humectant 5-20 parts, a preservative 0.1-0.5 parts, a thickening agent 0.1-1 part, a pH regulator 0.1-0.3 parts, an emulsifier 0.5-3 parts, and the rest is water. The composition selects a small-molecule peptide with biological activity, and is compounded with sophorolipid, 1,3-dihydroxyacetone, Poria cocos extract and a humectant to realize the functions of promoting tanning, accelerating tanning, prolonging tanning time, avoiding dry skin color and unevenness in the natural tanning process, and strong stability in long-time storage.

Description

Technical Field

[0001] This invention belongs to the field of personal care product technology, specifically relating to a melanin-promoting composition, its preparation method, and its application. Background Technology

[0002] Melanin is the core determinant of skin and hair color. Synthesized by melanocytes, its synthesis primarily relies on the catalytic action of tyrosinase, while being regulated by multiple factors including melanocyte-stimulating hormone (MSH) and the cellular metabolic environment. Melanin not only determines the natural attributes of skin and hair color, but also absorbs ultraviolet radiation, reducing UV damage to skin cell DNA and providing natural photoprotection. It can also improve issues such as vitiligo, gray hair, and pale skin prone to sunburn, meeting consumers' personalized skin and hair care needs.

[0003] Currently, most pigmentation-related products on the market focus on whitening and fading dark spots, while products that promote melanin production are relatively scarce. Furthermore, existing melanin-promoting products often suffer from technical shortcomings such as single-ingredient formulations, limited mechanisms of action, limited melanin-promoting effects, strong irritation, and poor biocompatibility. Among existing technologies, acetyl hexapeptide-1, as a biomimetic peptide, can mimic the function of MSH, binding to the MC1R receptor on the surface of melanocytes to activate tyrosinase activity and promote melanin synthesis. It is gentle and non-irritating, but its melanin-promoting efficiency still has room for improvement when used alone, and its skin penetration is limited. Poria cocos, a traditional Chinese medicine, contains triterpenoids in its extracts that can regulate tyrosinase activity and also has moisturizing, anti-inflammatory, and skin barrier-repairing effects, exhibiting excellent biocompatibility. However, the melanin-promoting effect of Poria cocos extract alone is weak and its onset of action is slow. Sophorolipids, as a natural biosurfactant, can promote activity... The ingredients are absorbed through the skin, improving the stability of the composition. They also have the advantages of low toxicity and biodegradability, but do not have a direct melanin-promoting function. 1,3-Dihydroxyacetone (DHA), as a naturally occurring small molecule ketone compound, not only has good skin compatibility, but can also undergo Maillard reactions with amino acids in the stratum corneum of the skin to form melanin-like substances, achieving an immediate melanin-promoting effect. At the same time, it can help regulate melanocyte metabolism and provide a good cellular environment for endogenous melanin synthesis. However, in the current technology, it has not been synergistically combined with acetyl hexapeptide-1, Poria cocos extract, and sophorolipid for the research and development of melanin-promoting products.

[0004] Therefore, developing a melanin-promoting composition that has significant synergistic effects, is gentle, safe, and long-lasting, and also has multiple skin care benefits has become a technical challenge that urgently needs to be solved in this field. Summary of the Invention

[0005] The first technical problem to be solved by this invention is to provide a tanning composition that can promote tanning, accelerate the darkening process, and prolong the tanning time, without causing dry skin or unevenness during the natural fading process, and exhibiting strong stability over long periods of time. The second technical problem to be solved by this invention is to provide a method for preparing the tanning composition that is simple to operate, has mild reaction conditions, and is easy to industrialize. The third technical problem to be solved by this invention is to provide the application of this compound in the preparation of tanning skin care products or hair darkening care products.

[0006] To solve the above-mentioned technical problems, the technical solution adopted by the present invention is as follows:

[0007] A melanin-promoting composition comprising, by weight percentage: 0.01-0.5 parts acetyl hexapeptide-1, 1-5 parts Poria cocos extract, 0.5-5 parts sophorolipid, 3-20 parts 1,3-dihydroxyacetone, 5-20 parts humectant, 0.1-0.5 parts preservative, 0.1-1 part thickener, 0.1-0.3 parts pH adjuster, 0.5-3 parts emulsifier, with the remainder being water.

[0008] Further, the melanin-promoting composition comprises, by weight percentage, the following components: 0.01-0.2 parts acetyl hexapeptide-1, 1-3 parts Poria cocos extract, 0.5-2 parts sophorolipid, 3-10 parts 1,3-dihydroxyacetone, 5-12 parts humectant, 0.1-0.3 parts preservative, 0.1-0.5 parts thickener, 0.1-0.2 parts pH adjuster, 0.5-1.5 parts emulsifier, and the remainder being water.

[0009] Further, the melanin-promoting composition comprises, by weight percentage, the following components: 0.2 parts acetyl hexapeptide-1, 3 parts Poria cocos extract, 2 parts sophorolipid, 10 parts 1,3-dihydroxyacetone, 12 parts humectant, 0.3 parts preservative, 0.5 parts thickener, 0.2 parts pH adjuster, 1.5 parts emulsifier, and the remainder being water.

[0010] Furthermore, the preparation method of the Poria cocos extract is as follows: take dried Poria cocos powder, add ethanol for reflux extraction, filter, and concentrate to obtain crude extract; extract the crude extract with chloroform, collect the organic phase, concentrate and dry to obtain the final product.

[0011] Further, the humectant is selected from one or more of glycerin, propylene glycol, and sodium hyaluronate; the preservative is selected from one or more of phenoxyethanol, methylparaben, and potassium sorbate; the thickener is selected from one or more of xanthan gum, carbomer, and hydroxyethyl cellulose; the pH adjuster is selected from one or two of triethanolamine and citric acid; and the emulsifier is Tween-80.

[0012] Furthermore, the method for preparing the aforementioned melanin-promoting composition includes the following steps:

[0013] 1) Pulverize and sieve the Poria cocos extract for later use; dissolve acetyl hexapeptide-1 in deionized water to prepare a stock solution for later use; mix 1,3-dihydroxyacetone with an emulsifier and stir until completely dispersed to prepare a DHA dispersion for later use.

[0014] 2) Take deionized water, add humectant and thickener, stir until completely dissolved to obtain the aqueous phase;

[0015] 3) Add Poria cocos extract and sophorolipid to the aqueous phase obtained in step 2) in sequence, stir well, then add acetyl hexapeptide-1, and finally add 1,3-dihydroxyacetone dispersion, and stir well.

[0016] 4) Add a pH adjuster to adjust the pH of the system, then add a preservative and continue stirring until homogeneous; then cool the mixture to room temperature, filter and degas to obtain the blackening composition.

[0017] Furthermore, in step 1), the reaction temperature is 70~80℃ and the temperature is maintained for 15~20min; in step 2), the stirring speed is 300~500r / min and the stirring time is 15~20min.

[0018] Furthermore, the application of the described hair-darkening composition in the preparation of hair-darkening skincare products or hair-darkening care products.

[0019] Furthermore, the hair-darkening skincare products include lotions, creams, serums, and toners, and the hair-darkening care products include shampoos, conditioners, and hair masks.

[0020] Furthermore, when the composition is used to prepare a hair-darkening skin care product, the amount added to the skin care product is 5-20% by weight; when the composition is used to prepare a hair-darkening care product, the amount added to the care product is 3-15% by weight.

[0021] Compared with the prior art, the present invention has the following advantages:

[0022] (1) The composition of the present invention selects bioactive small molecule peptides and combines them with sophorolipids, 1,3-dihydroxyacetone, Poria cocos extract and moisturizers to form a composition, so that the composition can promote tanning, accelerate the tanning process and prolong the tanning time, without causing dry skin or unevenness during the natural tanning process, and has strong stability after long-term storage.

[0023] (2) This invention uses natural Poria cocos extract, natural sophorolipid, food-grade / cosmetic-grade 1,3-dihydroxyacetone, combined with the mild synthetic peptide acetyl hexapeptide-1, avoiding the irritation of traditional melanin-promoting ingredients; at the same time, the repairing effect of Poria cocos extract, the mild properties of sophorolipid, and the low irritation of 1,3-dihydroxyacetone further reduce the irritation of the composition. After skin irritation test, there were no adverse reactions such as redness, swelling, and itching, making it suitable for all skin types, including sensitive skin; the biodegradability of sophorolipid further enhances the safety and environmental friendliness of the composition, and it is gentle to use for a long time.

[0024] (3) The preparation method of the present invention does not require complex equipment and processes, is easy to operate, has mild reaction conditions, and is easy to industrialize; at the same time, the dispersion problem of each component is solved by emulsification treatment, which improves the stability of the composition and can meet the needs of industrial production, storage and use. Attached Figure Description

[0025] Figure 1 The images show the coloring effects of different compositions of this application on the skin.

[0026] Figure 2 Photographs of skin color taken 31 hours after coloring with different compositions of this application;

[0027] Figure 3 This is a graph showing the effect of different compositions of this application on cell viability;

[0028] Figure 4 These are physical images of the specimens examined under different conditions for 90 days, as shown in Example 2 of this application.

[0029] Figure 5 This is a diagram showing the moisturizing effect on the skin at different times in Example 2 of this application. Detailed Implementation

[0030] The present invention will be further illustrated below with reference to specific embodiments. These embodiments are implemented based on the technical solutions of the present invention, and it should be understood that these embodiments are only used to illustrate the present invention and are not intended to limit the scope of the present invention.

[0031] The preparation method of Poria cocos extract used in the following examples includes: adding ethanol to dried Poria cocos powder, refluxing and extracting 2-3 times, each time for 1-2 hours, and combining all the extracts; filtering the combined extract to remove impurities, and then concentrating the filtered extract to obtain crude extract of Poria cocos extract.

[0032] The acetyl hexapeptide-1 used in Examples 1-3 and Comparative Examples 1-4 below was selected from the peptides of Nanjing Spaco Biotechnology Co., Ltd. ® AH1P, a sophorolipid, is produced using Sifu yeast from Nanjing Sibaico Biotechnology Co., Ltd. ®SLD600, 1,3-dihydroxyacetone (DHA), is produced using the Tansebao formula from Nanjing Sibaico Biotechnology Co., Ltd. ® DHA; Acetyl hexapeptide-1 used in Comparative Examples 5-6 was purchased from Genscript Biotech's Ruishuyan brand. TM Sophorolipids were purchased from Evonik SOPHANCE® LA-A, and 1,3-dihydroxyacetone (DHA) was purchased from Merck Dihydroxyacetone Extra Pure (DHA). Phenoxyethanol was obtained from Nanjing Spico Biotechnology Co., Ltd. ® PHE and xanthan gum are produced using Sifuyun from Nanjing Sibaico Biotechnology Co., Ltd. ® XTG200 uses food-grade glycerin from KLK, Malaysia; triethanolamine uses analytical grade AR triethanolamine from Sinopharm Chemical Reagent Co., Ltd.; and Tween-80 uses reagent-grade Tween-80 from Sinopharm Chemical Reagent Co., Ltd.

[0033] Example 1

[0034] A method for preparing a melanin-promoting composition, wherein the formula is as follows by mass percentage: 0.01 parts acetyl hexapeptide-1, 1 part Poria cocos extract, 0.5 parts sophorolipid, 3 parts 1,3-dihydroxyacetone (DHA), 5 parts glycerin, 0.1 parts phenoxyethanol, 0.1 parts xanthan gum, 0.1 parts triethanolamine, 0.5 parts Tween-80, and 89.69 parts deionized water.

[0035] Includes the following steps:

[0036] (1) Pulverize the Poria cocos extract through a 100-mesh sieve and set aside; dissolve acetyl hexapeptide-1 in 0.5 parts of deionized water to prepare a stock solution and set aside; mix 1,3-dihydroxyacetone (DHA) with Tween-80 and stir until completely dispersed to prepare a DHA dispersion and set aside.

[0037] (2) Take 89.69 parts of deionized water, add 5 parts of glycerol and 0.1 parts of xanthan gum, stir until completely dissolved, heat to 70°C at a rate of 5°C / min, keep warm for 15 min, sterilize, and cool to 45°C;

[0038] (3) Add 1 part of Poria cocos extract and 0.5 part of sophorolipid to the aqueous phase in sequence, stir evenly, then add acetyl hexapeptide-1 stock solution, continue stirring for 5 min, finally add DHA dispersion, stir at 300 r / min for 15 min, and simultaneously use 100W ultrasound to assist dispersion for 5 min.

[0039] (4) Add 0.1 parts of triethanolamine, adjust the pH of the system to 6.0, add 0.1 parts of phenoxyethanol, and continue stirring for 5 minutes; cool to room temperature, filter, and degas to obtain the blackening composition.

[0040] Example 2

[0041] A method for preparing a melanin-promoting composition, wherein the formula is as follows by mass percentage: 0.2 parts acetyl hexapeptide-1, 3 parts Poria cocos extract, 2 parts sophorolipid, 10 parts 1,3-dihydroxyacetone (DHA), 12 parts propylene glycol, 0.3 parts methylparaben, 0.5 parts carbomer, 0.2 parts citric acid, 1.5 parts Tween-80, and 70.3 parts deionized water.

[0042] Includes the following steps:

[0043] (1) Pulverize the Poria cocos extract through a 100-mesh sieve and set aside; dissolve acetyl hexapeptide-1 in 1 part of deionized water to prepare a stock solution and set aside; mix 1,3-dihydroxyacetone (DHA) with Tween-80 and stir until completely dispersed to prepare a DHA dispersion and set aside.

[0044] (2) Take 70.3 parts of deionized water, add 12 parts of propylene glycol and 0.5 parts of carbomer, stir until completely dissolved, heat to 75°C at a rate of 8°C / min, keep warm for 18 min, sterilize, and cool to 45°C;

[0045] (3) Add 3 parts of Poria cocos extract and 2 parts of sophorolipid to the aqueous phase in sequence, stir evenly, then add acetyl hexapeptide-1 stock solution, continue stirring for 8 min, and finally add 1,3-dihydroxyacetone (DHA) dispersion, stir at 400 r / min for 18 min, and simultaneously use 150W ultrasonic-assisted dispersion for 8 min.

[0046] (4) Add 0.2 parts of citric acid to adjust the pH of the system to 6.0, then add 0.3 parts of methylparaben and continue stirring for 5 minutes; cool to room temperature, filter and degas to obtain the blackening composition.

[0047] Example 3

[0048] A method for preparing a melanin-promoting composition, wherein the formula is as follows by mass percentage: 0.5 parts acetyl hexapeptide-1, 5 parts Poria cocos extract, 5 parts sophorolipid, 20 parts 1,3-dihydroxyacetone (DHA), 20 parts sodium hyaluronate, 0.5 parts potassium sorbate, 1 part hydroxyethyl cellulose, 0.3 parts triethanolamine, 3 parts Tween-80, and 44.7 parts deionized water.

[0049] Includes the following steps:

[0050] (1) Pulverize the Poria cocos extract through a 100-mesh sieve and set aside; dissolve acetyl hexapeptide-1 in 1 part of deionized water to prepare a stock solution and set aside; mix 1,3-dihydroxyacetone (DHA) with Tween-80 and stir until completely dispersed to prepare a DHA dispersion and set aside.

[0051] (2) Take 44.7 parts of deionized water, add 20 parts of sodium hyaluronate and 1 part of hydroxyethyl cellulose, stir until completely dissolved, heat to 80°C at a rate of 10°C / min, keep warm for 20 min, sterilize, and cool to 45°C.

[0052] (3) Add 5 parts of Poria cocos extract and 5 parts of sophorolipid to the aqueous phase in sequence, stir evenly, then add acetyl hexapeptide-1 stock solution, continue stirring for 10 min, and finally add 1,3-dihydroxyacetone (DHA) dispersion, stir at 500 r / min for 20 min, and simultaneously use 150W ultrasonic-assisted dispersion for 10 min.

[0053] (4) Add 0.3 parts of triethanolamine, adjust the pH of the system to 6.0, add 0.5 parts of potassium sorbate, and continue stirring for 10 minutes; cool to room temperature, filter, and degas to obtain the blackening composition.

[0054] Comparative Example 1

[0055] The difference from Example 2 is that no Poria cocos extract was added, and the melanin-promoting composition was prepared.

[0056] Comparative Example 2

[0057] The difference from Example 2 is that sophorolipids were not added, and a melanin-promoting composition was prepared.

[0058] Comparative Example 3

[0059] The difference from Example 2 is that 1,3-dihydroxyacetone (DHA) was not added to prepare the melanin-promoting composition.

[0060] Comparative Example 4

[0061] The difference from Example 2 is that acetyl hexapeptide-1 was not added, and the melanin-promoting composition was prepared.

[0062] Comparative Example 5

[0063] The difference from Example 2 is that commercially available sophorolipids were used to prepare the melanin-promoting composition.

[0064] Comparative Example 6

[0065] The difference from Example 2 is that commercially available common acetyl hexapeptide-1 was used to prepare the melanin-promoting composition.

[0066] Comparative Example 7

[0067] The difference from Example 2 is that commercially available 1,3-dihydroxyacetone was used to prepare the melanin-promoting composition.

[0068] The products prepared in Examples 1-3 and Comparative Examples 1-7 were subjected to performance tests.

[0069] 1. Skin irritation test

[0070] Test Methods: A 3D skin model was used, and the impact of the test samples on the tissue activity of the 3D skin model was evaluated according to the OECD TG 439 method. Result Interpretation: Tissue activity less than or equal to 50% indicated skin irritation; tissue activity greater than 50% indicated no skin irritation. Examples 1-3 and Comparative Examples 1-7 were tested, and the results are shown in Table 1.

[0071] Table 1. Skin irritation test results of the products prepared in Examples 1-3 and Comparative Examples 1-7.

[0072]

[0073] As can be seen from Table 1, the activity of Examples 1-2 and Comparative Examples 1-7 in the 3D skin model tissue was all above 80%, and they were non-irritating to the skin. The activity of Example 3 in the 3D skin model tissue was 57.41%, which was lower than that of Examples 1-2 and Comparative Examples 1-7.

[0074] 2. Coloring effect test

[0075] Skin pigmentation is characterized by ITA°. The smaller the ITA°, the darker the skin tone. ITA° (Individual Typology Angle) is an objective indicator of skin color depth. It is a parameter that characterizes human skin color by calculating specific light parameters reflected by the skin and quantifying skin color in terms of angle values ​​(-90° to +90°). The larger the value (closer to +90°), the lighter the skin tone, and the smaller the value (closer to -90°), the darker the skin tone.

[0076] Test Method: Thirty normal subjects were selected for a forearm staining experiment using a C-CUBE instrument. Test Procedure: The arms were cleaned and rested for 20 minutes in a constant temperature (20℃-25℃) and constant humidity (40%-60%) environment; three test sites were marked on the inner forearm; data were collected at 0 h; the corresponding sample (0.3g±0.01g) was applied to each site and gently pressed until absorbed; data were collected at 3h, 7h, 24h, 31h, 48h, 72h, 96h, and 175h. Experimental results are shown in Table 2 and... Figure 1-2 As shown.

[0077] Table 2. Test results of coloring effect of products prepared in Examples 1-3 and Comparative Examples 1-7

[0078]

[0079] From Table 2 and Figure 1-2It can be seen that, compared with other examples, the ITA° measured at the same time was smaller and the skin color was darker, indicating that the composition has the best coloring effect on the skin.

[0080] 3. Cytotoxicity test

[0081] Assay method: Cell viability was detected by MTT assay. The effect of different samples on the survival rate of mouse embryonic fibroblasts (NIH / 3T3) was tested at a concentration of 0.1 mg / mL for 48 hours.

[0082] Test Procedure: Collect NIH / 3T3 cells in the logarithmic growth phase. Add 50 μL of cell suspension to each well of a 96-well plate, with a cell count of 5000 cells / well (margin wells are filled with sterile PBS). Incubate overnight. After incubation, add 50 μL of the test sample to each well and incubate for 48 hours. Then add 20 μL of MTT solution (5 mg / mL) to each well and continue incubation for 3 hours. After incubation, carefully aspirate the culture medium from the wells. Add 150 μL of dimethyl sulfoxide to each well and shake on a shaker at low speed for 10 minutes to fully dissolve any crystals. Measure the absorbance of each well at 490 nm using an ELISA reader to indirectly reflect the number of viable cells. Result Calculation:

[0083] Cell viability % = [OD(sample) - OD(blank)] / [OD(solvent control) - OD(blank)] × 100%

[0084] The test results are as follows Figure 3 As shown, all test samples were not toxic to cells, and the cell survival rate was above 90%.

[0085] 4. Stability Test

[0086] The test method involved dispensing the compositions obtained in Examples 1-3 and Comparative Examples 1-7 into test bottles and observing them under the following conditions for 90 days. The specific performance and test conditions are shown in Table 3.

[0087] Table 3. Stability test results of the products prepared in Examples 1-3 and Comparative Examples 1-7

[0088]

[0089] From Table 3 and Figure 4 It can be seen that the compositions of Examples 1-2 and Comparative Examples 1-7 are stable and without abnormalities under room temperature and accelerated testing conditions. The color of Example 3 is slightly darker under long-term light exposure, but there are no abnormalities under other conditions.

[0090] 5. Moisturizing performance test

[0091] Test Method: The moisture content of the stratum corneum of human skin was determined according to the "Guideline for Evaluation of Moisturizing Efficacy of Cosmetics" QB-T 4256-2011. Thirty normal subjects were selected for the forearm moisturizing test experiment. The testing instrument was a MoistureMeter SC (stratum corneum water content analyzer). Test Procedure: The arm was cleaned and rested for 20 minutes in a constant temperature (20℃-25℃) and constant humidity (40%-60%) environment; two test sites were marked on the inner side of the forearm; data were collected at 0 h; the corresponding sample (0.014 g / cm³) was applied to each site. 2 Press gently until absorbed; data were collected at 0h, 1h, 3h, 5h, and 7h. Results are shown in Table 4. Figure 5 As shown.

[0092] Table 4. Changes in stratum corneum moisture content at different times

[0093]

[0094] From Table 4 and Figure 5 It is known that the melanin-promoting composition of Example 2 of this application has excellent long-lasting moisturizing properties. After application, the moisture content of the stratum corneum of the skin continuously increases over time, gradually increasing from 29.5% at 0h to 34.3% at 1h, 38.1% at 3h, 40.4% at 5h, and reaching 47.1% at 7h. The moisture content increases by 17.6% within 7h, and there is no significant decay throughout the process. This indicates that the moisturizing ingredients in the composition work synergistically with Poria cocos extract and sophorolipids, which can not only quickly replenish moisture but also lock in moisture for a long time by repairing the skin barrier. This can effectively avoid skin dryness during the melanin-promoting and fading process, while providing a stable skin environment for melanin synthesis and uniform coloring, thus taking into account both the melanin-promoting effect and the skin care experience.

[0095] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A skin-promoting composition, characterized in that: The melanin-promoting composition comprises, by weight percentage: 0.01-0.5 parts acetyl hexapeptide-1, 1-5 parts Poria cocos extract, 0.5-5 parts sophorolipid, 3-20 parts 1,3-dihydroxyacetone, 5-20 parts humectant, 0.1-0.5 parts preservative, 0.1-1 part thickener, 0.1-0.3 parts pH adjuster, 0.5-3 parts emulsifier, and the remainder being water.

2. The skin-promoting composition according to claim 1, characterized in that: The melanin-promoting composition comprises, by weight percentage, the following components: 0.01-0.2 parts acetyl hexapeptide-1, 1-3 parts Poria cocos extract, 0.5-2 parts sophorolipid, 3-10 parts 1,3-dihydroxyacetone, 5-12 parts humectant, 0.1-0.3 parts preservative, 0.1-0.5 parts thickener, 0.1-0.2 parts pH adjuster, 0.5-1.5 parts emulsifier, and the remainder being water.

3. The skin-promoting composition according to claim 2, characterized in that: The melanin-promoting composition comprises, by weight percentage, the following components: 0.2 parts acetyl hexapeptide-1, 3 parts Poria cocos extract, 2 parts sophorolipid, 10 parts 1,3-dihydroxyacetone, 12 parts humectant, 0.3 parts preservative, 0.5 parts thickener, 0.2 parts pH adjuster, 1.5 parts emulsifier, and the remainder being water.

4. The skin-promoting composition according to claim 1, characterized in that: The preparation method of the Poria cocos extract is as follows: take dried Poria cocos powder, add ethanol for reflux extraction, filter, and concentrate to obtain crude extract; extract the crude extract with chloroform, collect the organic phase, concentrate and dry to obtain the final product.

5. The skin-promoting composition according to claim 1, characterized in that: The humectant is selected from one or more of glycerin, propylene glycol, and sodium hyaluronate; the preservative is selected from one or more of phenoxyethanol, methylparaben, and potassium sorbate; the thickener is selected from one or more of xanthan gum, carbomer, and hydroxyethyl cellulose; the pH adjuster is selected from one or two of triethanolamine and citric acid; and the emulsifier is Tween-80.

6. A method for preparing the melanin-promoting composition according to any one of claims 1-5, characterized in that: Includes the following steps: (1) Pulverize and sieve the Poria cocos extract for later use; dissolve acetyl hexapeptide-1 in deionized water to prepare a stock solution for later use; mix 1,3-dihydroxyacetone with an emulsifier and stir until completely dispersed to prepare a DHA dispersion for later use. (2) Take deionized water, add humectant and thickener, stir until completely dissolved to obtain the aqueous phase; (3) Add Poria cocos extract and sophorolipid to the aqueous phase obtained in step (2) in sequence, stir evenly, then add acetyl hexapeptide-1, and finally add 1,3-dihydroxyacetone dispersion, and stir evenly. (4) Add pH adjuster to adjust the pH of the system, then add preservative and continue to stir evenly; then cool the mixture to room temperature, filter and degas to obtain the blackening composition.

7. The method for preparing the skin-promoting composition according to claim 6, characterized in that: In step (1), the reaction temperature is 70~80℃ and the temperature is maintained for 15~20min; in step (2), the stirring speed is 300~500r / min and the stirring time is 15~20min.

8. The use of the hair-darkening composition according to any one of claims 1-5 in the preparation of hair-darkening skin care products or hair-darkening products.

9. The application according to claim 8, characterized in that: The hair-darkening skincare products include lotions, creams, serums, and toners, while the hair-darkening care products include shampoos, conditioners, and hair masks.

10. The application according to claim 8, characterized in that: When the composition is used to prepare a hair-darkening skin care product, the amount added to the skin care product is 5-20% by weight; when the composition is used to prepare a hair-darkening care product, the amount added to the care product is 3-15% by weight.

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