Use of cistanche tuber juice in preparation of anti-aging preparations

Abstract

The application relates to the technical field of health food, in particular to application of Cistanche tuber raw juice in preparation of anti-aging preparations. The application of Cistanche tuber raw juice in preparation of anti-aging preparations, wherein the components of the Cistanche tuber raw juice include Cistanche tuber and Momordica grosvenori. The Cistanche tuber raw juice can significantly enhance the muscle strength of mice, improve the motion coordination and balance of the mice, and improve the survival rate, and better effects are achieved than those of using the same dose of Cistanche tuber water solution alone, so that the Cistanche tuber raw juice has better anti-aging effects and is expected to be applied in clinical application.

Description

Technical Field

[0001] This invention relates to the field of health food technology, and in particular to the application of Cistanche deserticola extract in the preparation of anti-aging agents. Background Technology

[0002] Biologically, aging is a spontaneous and inevitable process that occurs naturally over time. It is a complex natural phenomenon characterized by structural degeneration, functional decline, and reduced adaptability and resistance. Physiologically, aging is viewed as the individual's developmental history, beginning with fertilization and continuing into old age. Pathologically, aging results from stress and strain, injury and infection, weakened immune responses, nutritional imbalances, metabolic disorders, and the accumulation of neglect and misuse of drugs. In short, aging is the inevitable outcome of the combined effects of many pathological, physiological, and psychological processes; it is a process of loss and degeneration of the body's constituent substances, tissue structure, and physiological functions. With an aging population, the incidence of age-related chronic diseases, such as Parkinson's disease (PD), Alzheimer's disease (AD), and malignant tumors, continues to rise, seriously affecting human health and quality of life. Therefore, slowing down the aging process has become a major focus in the exploration of the mysteries of life.

[0003] Cistanche deserticola is a plant used for both medicinal and culinary purposes, possessing high medicinal value. Long-term consumption of Cistanche deserticola can enhance the body's resistance and promote a healthy and strong physique. Cistanche deserticola has a certain degree of anti-aging effect, regulates endocrine function, promotes metabolism, and has a strengthening effect. It also has a regulatory effect on the decrease and increase of liver and spleen nucleic acid content in animals with Yang deficiency and Yin deficiency, and simultaneously activates the adrenal glands and releases corticosteroids. Patent (CN202510016593) discloses a composition containing Cistanche deserticola that can improve skin elasticity and anti-aging ability, and more effectively regulate the biological activity within the skin.

[0004] Monk fruit is one of the first batch of medicinal and edible materials approved by the state. It has a sweet taste and cool nature, and enters the lung and large intestine meridians. It has the effects of clearing heat and cooling blood, promoting body fluid production and relieving cough, lubricating the intestines and detoxifying, beautifying the skin and improving complexion, and moistening the lungs and resolving phlegm. It can be used to prolong life, maintain youthful appearance, and treat phlegm-heat cough, sore throat, constipation, thirst, and irritability. Modern medicine has proven that monk fruit has significant effects on bronchitis, hypertension, and other diseases, and can also play a role in preventing and treating coronary heart disease, arteriosclerosis, and obesity. Patent (CN202311690346) discloses a fermented monk fruit juice with weight loss, energy-boosting, and antioxidant and anti-aging effects.

[0005] The Cistanche deserticola extract was developed by the Science and Technology Innovation Research Institute under Gansu Pharmaceutical Group. It is made from Cistanche deserticola and Luo Han Guo as the main ingredients in an appropriate ratio. This invention discloses a new use for the Cistanche deserticola extract. Summary of the Invention

[0006] The purpose of this invention is to provide a new use for Cistanche deserticola extract in the preparation of anti-aging agents.

[0007] To achieve the above-mentioned objectives, the present invention provides the following technical solution: This invention provides the application of Cistanche deserticola extract in the preparation of anti-aging agents, wherein the components of the Cistanche deserticola extract include Cistanche deserticola and Siraitia grosvenorii.

[0008] Preferably, the Cistanche deserticola extract also includes food- and / or pharmaceutically acceptable excipients.

[0009] Preferably, the dosage form of the preparation includes tablets, sprays, granules, capsules, oral liquids, and injections.

[0010] Beneficial effects: This invention provides a novel use of Cistanche deserticola extract in the preparation of drugs and health products with anti-aging effects. Specifically, the aging is an doxorubicin-induced aging model. The Cistanche deserticola extract can significantly enhance the muscle strength of mice, improve their motor coordination and balance, and increase their survival rate. It achieves better results than using the same dose of Cistanche deserticola aqueous solution alone. Therefore, Cistanche deserticola extract has a better anti-aging effect and is expected to be used in clinical applications. Attached Figure Description

[0011] Figure 1 These are the results of a mouse grip strength test. Figure 2 These are the results of the mouse pole climbing test; Figure 3 These are the test results from a rotating bar fatigue tester; Figure 4 The survival curve for mice is shown. Detailed Implementation

[0012] This invention provides the application of Cistanche deserticola extract in the preparation of anti-aging agents, wherein the components of the Cistanche deserticola extract include Cistanche deserticola and Siraitia grosvenorii.

[0013] In this invention, the Cistanche deserticola extract also includes food- and / or pharmaceutically acceptable excipients.

[0014] In this invention, the dosage forms of the preparation include tablets, sprays, granules, capsules, oral liquids, and injections.

[0015] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0016] Example 1

[0017] Twenty-four clean-grade male Kunming mice (four weeks old, weighing 22±2g, from the Experimental Animal Building of Lanzhou University) were acclimatized for one week under conditions of 20-25℃ and a regular 12h / 12h day / night cycle. Then, they were randomly divided into four groups: control group (Control), model control group (Model), Cistanche deserticola group (M+MCR), and Cistanche deserticola extract group (M+MCRJ), with six mice in each group. Mice in the control group and model control group were fed physiological saline; Cistanche deserticola group: 5% Cistanche deserticola aqueous solution was diluted with water at a volume ratio of 1:10 into drinking water; Cistanche deserticola puree group: Cistanche deserticola puree was diluted with water at a volume ratio of 1:10 into drinking water; mice were allowed free access to water; The preparation method of the 5% Cistanche deserticola aqueous solution is as follows: Cistanche deserticola slices are dried in an oven at 60℃, ground into powder using a grinder, and then dissolved in physiological saline to prepare a 5% Cistanche deserticola aqueous solution (prepared and used immediately); Cistanche deserticola raw pulp (purchased from Gansu Pharmaceutical Group Co., Ltd.) was developed by the Science and Technology Innovation Research Institute under Gansu Pharmaceutical Group. The components of Cistanche deserticola raw pulp include Cistanche deserticola and Luo Han Guo (monk fruit); After one week of feeding, except for the blank control group, the mice in the other groups were intraperitoneally injected with doxorubicin (purchased from Shanghai Mairui Biochemical Technology Co., Ltd., CAS: 25316-40-9) at a dose of 3 mg / kg, once every three days, for a total of 5 injections. The blank control group mice were injected with the same dose of physiological saline. Except for the day of doxorubicin injection, the mice were fed normally with 5% Cistanche deserticola aqueous solution or Cistanche deserticola raw juice every day. The blank control group and the model group mice had free access to water until the last dose of doxorubicin was administered. Behavioral tests were performed on mice after they were injected with doxorubicin, focusing on motor and cognitive aspects: (1) Grip test Following the fourth dose of doxorubicin, all mice underwent a grip strength test: Mice were placed at the measurement location of the grip strength meter, trained to grasp the measuring grid with all four limbs, and their maximum reading at the moment of force application was recorded; this reading represented the animal's grip strength. Three, but not consecutive, measurements were taken, and the average was calculated to obtain more stable and reliable data for assessing the mice's muscle strength. The results are as follows: Figure 1 As shown; The results showed that, compared with the blank control group, the model group mice had weakened muscle strength, consistent with the doxorubicin-induced aging model, indicating that the model was successfully constructed. p < 0.001). Compared with the Cistanche deserticola group, the Cistanche deserticola extract group showed better results in improving the grip strength and enhancing muscle strength of mice than Cistanche deserticola alone. p < 0.05). Compared with the model group, the Cistanche deserticola extract group significantly enhanced the grip strength of mice ( p<0.05), which has the effect of reversing the muscle weakness induced by doxorubicin in an aging mouse model.

[0018] (2) Pole climbing test

[0019] The pole climbing test is an essential tool for studying animal movement and balance. It is a simple, non-invasive method that aims to assess the limb movement or muscle function of rodents. After the fifth dose of doxorubicin, all mice underwent a pole-climbing test. During the test, the mice were placed at the top of the pole with their heads down, and the time it took for the mice to climb from the top to the bottom (with all four paws on the ground) was recorded. Each mouse was tested three times, but not consecutively. The results are as follows: Figure 2 As shown; The results showed that, compared with the blank control group, the time taken for mice in the model group to climb from the top of the pole to the bottom was significantly longer. p The value <0.01 indicates that doxorubicin-induced aging mice exhibited slower reaction times, reduced limb coordination, and weakened motor function, confirming the successful establishment of the model. Compared to the model group, both Cistanche deserticola extract and Cistanche deserticola improved motor function and limb coordination in aging mice and shortened pole-climbing time, but the effects of the two were not significantly different. p =0.084). However, the time taken for mice in the Cistanche deserticola extract group to climb the pole was significantly shorter than that in the model group ( p <0.05), Cistanche deserticola extract can improve motor coordination and limb flexibility in aging mice.

[0020] (3) Rotary bar fatigue test

[0021] The swivel test is used to assess the motor coordination and balance of rodents: the animal must maintain balance on a rotating bar, and the instrument records the time required for the animal to fall from the bar at different speeds or at continuously accelerating speeds, the speed of the bar when it falls, and the distance the animal travels. After the fifth dose of doxorubicin, all mice underwent a rotarod fatigue test. One mouse was placed in each lane, and after all mice on the bar were kept balanced, the start button was pressed to begin the test. The bar initially rotated at a constant speed of 5 rpm, gradually accelerating from 5 rpm to 30 rpm over 60 seconds. The time it took for each mouse to fall off the bar was recorded. Each mouse was tested three times, but not consecutively. The results are as follows: Figure 3 As shown; The results showed that compared with the blank control group, the time of the model group mice rotating on the wooden stick was shortened, but both Cistanche deserticola extract and Cistanche deserticola could prolong the time the mice stayed on the wooden stick, and the time of the Cistanche deserticola extract group mice rotating on the wooden stick was longer than that of the model group and the Cistanche deserticola group.

[0022] (4) Evaluation of mouse survival days

[0023] The mice's phenotypic changes were observed daily, and the number of surviving mice was counted daily to calculate the survival rate. The results are as follows: Figure 4 As shown; Compared with the blank control group, the mice in the model group had slowed movement, rough and dull fur, shortened lifespan and increased mortality; the mice in the Cistanche deserticola extract group had better lifespan and survival rate than the model group and the Cistanche deserticola group.

[0024] As can be seen from the above embodiments, the present invention provides the application of Cistanche deserticola extract in the preparation of anti-aging agents.

[0025] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. Application of Cistanche deserticola extract in the preparation of anti-aging agents, wherein the components of the Cistanche deserticola extract include Cistanche deserticola and Siraitia grosvenorii.

2. The application according to claim 1, characterized in that, The Cistanche deserticola extract also includes food- and / or pharmaceutically acceptable excipients.

3. The application according to claim 1, characterized in that, The dosage forms of the preparations include tablets, sprays, granules, capsules, oral liquids, and injections.

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