A pueraria montana lobata cranberry composition, oral preparation and application thereof

Abstract

The present application provides a kind of kudzu vine cranberry composition for protecting liver from alcohol, which contains the following ingredients by weight: 30-50 parts of extract of Hovenia dulcis Thunb, 30-50 parts of mixed extract, 20-40 parts of extract of Pueraria lobata, 10-20 parts of extract of Poria cocos, 10-20 parts of extract of Glycyrrhiza uralensis, 0.05-0.1 parts of vitamin C; the raw materials of the mixed extract are mixed by cranberry and Fructus Lycii in a mass ratio of (1-2):(1-2). The present application further provides formulations and food containing the composition. The present application further protects the use of the composition or formulation in preparing health food or medicine with auxiliary protective effect on chemical liver injury. The composition provided by the present application can improve the activity of alcohol dehydrogenase / acetaldehyde dehydrogenase, reduce the content of MDA in tissues and increase the content of GSH, and reduce the content of triglyceride in liver cells, thereby comprehensively exerting the effect of protecting liver from alcohol, with high safety, small side effects, and wide sources of raw materials, and having important market value and development prospect.

Description

Technical Field

[0001] This invention belongs to the field of traditional Chinese medicine and food technology, specifically relating to a kudzu root and cranberry composition and an oral preparation, as well as the application of the composition and preparation in the preparation of food, health food and medicine. Technical Background

[0002] Alcoholic liver injury is a series of organic lesions caused by long-term, excessive alcohol consumption, resulting in persistent chronic damage to the liver. It is one of the most prevalent and widespread types of liver disease globally. Its pathological progression is gradual, initially manifesting as reversible alcoholic fatty liver. With continued alcohol consumption, it can rapidly progress to alcoholic hepatitis, followed by liver fibrosis. Once collagen fibers are widely deposited in the liver and form pseudolobules, it enters the irreversible stage of cirrhosis.

[0003] Currently, the active ingredients used in the market to assist in the protection against alcoholic liver injury mainly focus on silymarin, glutathione, and dihydromyricetin. Although silymarin preparations have a stable protective effect on biomembranes, they are not very effective in improving acute oxidative stress and intestinal barrier function damage caused by alcohol metabolism. Existing patented technologies involving kudzu root mostly focus on using puerarin to increase the activity of alcohol dehydrogenase and aldehyde dehydrogenase to promote alcohol metabolism. However, practical applications show that simply promoting metabolism often leads to the instantaneous and massive generation of metabolic intermediates, which can easily induce a coupling effect of "accelerated metabolism - oxidative damage," causing secondary damage.

[0004] Therefore, existing single-component or simple compound formulations are insufficient to effectively protect against alcoholic liver injury across the entire chain, leaving a significant technological gap in multi-target synergistic effects. Consequently, developing a composition capable of systematically intervening in the multi-dimensional pathological processes of alcoholic liver injury and exhibiting significant synergistic effects has become a critical technological challenge that urgently needs to be addressed in this field. Summary of the Invention

[0005] In order to overcome the shortcomings of the prior art, the present invention provides a traditional Chinese medicine composition, preparation and application in related products that have a multi-pathway synergistic effect in assisting protection against liver injury.

[0006] Specifically, the present invention provides a kudzu root and cranberry composition for relieving hangovers and protecting the liver, comprising the following components in parts by weight: 30-50 parts of Hovenia dulcis extract, 30-50 parts of mixed extract, 20-40 parts of kudzu root extract, 10-20 parts of Poria cocos extract, 10-20 parts of licorice extract, and 0.05-0.1 parts of vitamin C; wherein the raw material of the mixed extract is cranberry and wolfberry mixed in a mass ratio of (1-2):(1-2).

[0007] The extracts described in the present invention are all obtained by mixing traditional Chinese medicine raw materials and water in a mass ratio of 1:20, extracting, filtering insoluble substances, collecting the filtrate, and drying.

[0008] The composition provided by the present invention follows the prescription principle of traditional Chinese medicine in terms of nature, flavor, and meridian tropism. Specifically, in the composition provided by the present invention, Hovenia dulcis for relieving hangover and protecting the liver is the monarch drug; wolfberry and cranberry for soothe the liver and moisten the liver are the ministerial drugs; kudzu root for invigorating the spleen and promoting the production of body fluid, Poria cocos and licorice for invigorating the spleen and replenishing qi, promoting diuresis and relieving hangover toxicity, are both assistant drugs; vitamin C tastes sour and enters the liver as the guiding drug. On this basis, the present invention optimizes the dosage of each component in the composition, so that the components act together to achieve the effects of synergistic enhancement and restraint balance.

[0009] In the composition provided by the present invention, the extract of Hovenia dulcis, the "monarch drug", and the extracts of kudzu root, Poria cocos, and licorice, the "assistant drugs", are all extracted by the single extraction method, that is, the single traditional Chinese medicine raw materials are independently taken for separate extraction. After obtaining the extracts corresponding to each raw material respectively, they are then mixed to form a composition; while the mixed extract of cranberry and wolfberry, the "ministerial drugs", is extracted by the mixed extraction method, that is, multiple traditional Chinese medicine raw materials are mixed and then mixed for extraction. Through a large number of practices, the present invention finds that for the formula provided by the present invention, mixing cranberry and wolfberry first and then extracting with hot water achieves a significant effect of promoting the dissolution of each other's specific active ingredients during the extraction process. The obtained extract, when combined with the "monarch", "assistant", and "guiding" in the formula, has a significantly better hangover and liver protection effect than the composition obtained by separately extracting each raw material or mixing all the raw materials for extraction.

[0010] The present invention further finds that for the "ministerial drugs" in the formula, on the basis of the mixed extract of cranberry and wolfberry, adding chrysanthemum further and controlling the ratio of the three to (1-2):(1-2):(1-2), the obtained mixed extract, when combined with the "monarch", "assistant", and "guiding" in the formula, can further enhance the auxiliary protection effect on alcoholic liver injury.

[0011] As a preferred embodiment of the present invention, in the composition, the best effect is obtained when cranberry, wolfberry, and chrysanthemum are mixed in a mass ratio of 2:(1-1.5):(1-1.5) and then extracted to obtain a mixed extract.

[0012] As a preferred scheme of the present invention, the "assistant drugs" in the composition can further include the extract of Astragalus membranaceus, the extract of Chinese yam, the extract of hawthorn, the extract of tangerine peel, and corn oligopeptide powder. The above components and Poria cocos and licorice together achieve invigorating the spleen and replenishing qi, promoting diuresis and relieving hangover toxicity, and combined with the effect of kudzu root in invigorating the spleen and promoting the production of body fluid, the efficacy of the overall "assistant" in the formula is enhanced.

[0013] In a preferred embodiment of the present invention, the "adjuvant" in the composition comprises the following components in parts by weight: 20-40 parts of kudzu root extract, 10-20 parts of poria cocos extract, 10-20 parts of licorice extract, 5-10 parts of astragalus extract, 5-10 parts of yam extract, 3-6 parts of hawthorn extract, 3-6 parts of tangerine peel extract, and 3-6 parts of corn oligopeptide powder.

[0014] In a preferred embodiment of the present invention, the composition comprises the following components in parts by weight: 40-45 parts of Hovenia dulcis extract, 40-45 parts of a mixed extract obtained by mixing and extracting cranberry, wolfberry and chrysanthemum in a mass ratio of 2:(1-1.5):(1-1.5), 35-40 parts of kudzu root extract, 10-15 parts of Poria cocos extract, 10-15 parts of licorice extract, 5-8 parts of Astragalus membranaceus extract, 8-10 parts of Dioscorea opposita extract, 4-5 parts of hawthorn extract, 3-4 parts of tangerine peel extract, 3-4 parts of corn oligopeptide powder, and 0.05-0.1 parts of vitamin C.

[0015] The raw material used in this invention, Hovenia dulcis seeds, is a plant of the Rhamnaceae family (Hovenia dulcis). Hovenia acerbica The mature seeds of *Hovenia dulcis* (Lindl.) are traditionally considered both a food and a medicinal herb. According to the *Tang Materia Medica*, they are "sweet, neutral, and non-toxic," while the *New Materia Medica* states they "enter the heart and spleen meridians." They are believed to have the effects of relieving alcohol poisoning, quenching thirst and relieving irritability, stopping vomiting, and promoting urination and defecation.

[0016] The raw material used in this invention is cranberry, an evergreen dwarf shrub belonging to the genus Vaccinium in the family Ericaceae. Blueberry macrocarpon Cranberries are the fruit of the cranberry family. Cranberries contain proanthocyanidins and other components, which have benefits such as preventing urinary tract infections, cardiovascular disease, protecting teeth, antibacterial properties, antioxidant effects, and lowering blood pressure.

[0017] The raw material used in this invention is Lycium barbarum, a plant belonging to the Solanaceae family (Lycium barbarum var. ningxiaense). Lycian barbarian The dried, ripe fruit of *Lycium barbarum* (L.) is a substance traditionally used as both food and a Chinese medicinal herb. According to the pharmacopoeia, wolfberries are sweet and neutral in nature, and enter the liver and kidney meridians. They have the effects of nourishing the liver and kidneys, benefiting essence and improving eyesight.

[0018] The chrysanthemum used in this invention is a plant of the Asteraceae family. Chrysanthemum morifolium The dried flower heads of *Ramat.* are traditionally used as both food and medicinal material. According to the pharmacopoeia, chrysanthemum is bitter and slightly cold in nature; it enters the lung and liver meridians. It is used for wind-heat colds, headaches and dizziness, red and swollen eyes, blurred vision, and carbuncles and boils.

[0019] The raw material used in this invention is kudzu root, a legume (Pueraria lobata). Lobed PuerariaThe dried root of *Kudzu* (Willd.) Ohwi is a substance traditionally used as both food and a Chinese medicinal herb. According to the pharmacopoeia, kudzu root is sweet and pungent in taste; cool in nature; and enters the spleen, stomach, and lung meridians. It has the effects of relieving muscle tension and reducing fever, promoting body fluid production and quenching thirst, promoting rash eruption, raising yang and stopping diarrhea, clearing the meridians and activating collaterals, and detoxifying alcohol.

[0020] The raw material used in this invention is Poria cocos, a fungus belonging to the Polyporaceae family. Poria cocos The dried sclerotium of *Poria cocos* (Schw.) Wolf is a substance traditionally used as both food and a Chinese medicinal herb. According to the pharmacopoeia, *Poria cocos* is sweet and bland in taste, and neutral in nature; it enters the heart, lung, spleen, and kidney meridians. It has the effects of promoting diuresis and eliminating dampness, strengthening the spleen, and calming the mind.

[0021] The licorice used in this invention is licorice root, a plant belonging to the legume family. Glycyrrhiza uralensis Fisch., Licorice inflata Inflated licorice Bat. or Licorice root Glycyrrhiza glabra The dried roots and rhizomes of *Licorice* are traditionally considered both a food and a medicinal herb. According to the pharmacopoeia, licorice is neutral in nature and enters the heart, lung, spleen, and stomach meridians. It has the effects of tonifying the spleen and replenishing qi, clearing heat and detoxifying, resolving phlegm and relieving cough, relieving spasms and pain, and harmonizing other herbs.

[0022] The raw material used in this invention is Astragalus membranaceus, a legume. Astragalus membranaceus (Fisch.) Bge. var. Mongolian (Bge.) Hsiao or Astragalus membranaceus Astragalus membranaceus The dried root of Astragalus membranaceus (Fisch.) Bge. is a substance traditionally used as both food and a Chinese medicinal herb. According to the pharmacopoeia, Astragalus membranaceus is sweet and slightly warm in nature, and enters the lung and spleen meridians. It has the effects of tonifying qi and raising yang, consolidating the exterior and stopping sweating, promoting diuresis and reducing swelling, generating fluids and nourishing blood, promoting circulation and relieving pain, promoting pus drainage and detoxification, and promoting wound healing and tissue regeneration.

[0023] The raw material used in this invention is yam, a plant of the Dioscoreaceae family. Dioscorea opposita The dried rhizome of *Thunb.* is a substance traditionally used as both food and a Chinese medicinal herb. According to the pharmacopoeia, yam is sweet and neutral in nature, and enters the spleen, lung, and kidney meridians. It has the effects of tonifying the spleen and stomach, promoting body fluid production and benefiting the lungs, and tonifying the kidneys and astringing essence.

[0024] The raw material used in this invention is hawthorn, a plant of the Rosaceae family. Hawthorn pinnatifida Bge.var. older NE Br. or hawthorn Hawthorn pinnatifida The dried, ripe fruit of *Begonia granatum* is traditionally considered both a food and a medicinal herb. According to the pharmacopoeia, hawthorn is sour, sweet, and slightly warm in nature, and enters the spleen, stomach, and liver meridians. It has the effects of aiding digestion, promoting qi circulation, dispersing blood stasis, and reducing turbidity and lipids.

[0025] The raw material used in this invention is dried tangerine peel, a plant of the Rutaceae family. Citrus reticulataThe dried, mature peel of Blanco and its cultivated varieties is traditionally considered both a food and a medicinal herb. According to the pharmacopoeia, dried tangerine peel is bitter, pungent, and warm in nature, and enters the lung and spleen meridians. It has the effects of regulating qi and strengthening the spleen, drying dampness and resolving phlegm.

[0026] The corn oligopeptide powder used in this invention is a new food ingredient (new resource food). It is prepared from corn protein powder through processes such as slurry preparation, enzymatic hydrolysis, separation, filtration, and spray drying. Its peptides consist of 2-8 amino acids, with an average molecular weight of less than 1000 Da. Animal and human trials have verified that corn oligopeptide powder has bioactivities such as antioxidant, antihypertensive, immune-enhancing, and liver-protective effects.

[0027] In a second aspect, the present invention provides an oral preparation of kudzu root and cranberry, comprising the kudzu root and cranberry composition described in the first aspect, and an appropriate amount of excipients acceptable in the food, health food, or pharmaceutical fields.

[0028] In one specific embodiment of the present invention, the oral preparation is a tablet, preferably a regular tablet, lozenge, chewable tablet, dispersible tablet or effervescent tablet.

[0029] In one specific embodiment of the present invention, the oral preparation is a capsule, preferably a hard capsule or a soft capsule.

[0030] In one specific embodiment of the present invention, the oral preparation is a granule, preferably a soluble granule, a suspension granule, or an effervescent granule.

[0031] In one specific embodiment of the present invention, the oral preparation is a powder.

[0032] In one specific embodiment of the present invention, the oral preparation is a paste.

[0033] In one specific embodiment of the present invention, the oral preparation is a candy, preferably selected from hard candies, hard-filled candies, cream candies, gel candies, gum-based candies, aerated candies, or compressed candies. When the oral preparation is a compressed candy, xylitol is preferably used as the sweetener.

[0034] In one specific embodiment of the present invention, the oral preparation is a liquid preparation, preferably a syrup, oral liquid or beverage.

[0035] Thirdly, the present invention provides a food product comprising the kudzu root and cranberry composition of the first aspect or the kudzu root and cranberry oral preparation of the second aspect.

[0036] The composition provided by this invention uses raw materials from substances that are both food and traditional Chinese medicine as published by health administration departments, new food raw materials (new resource foods), and foods or condiments consumed in daily life. It can be used in food, has high safety, and low toxicity and side effects.

[0037] Fourthly, the present invention provides the use of the kudzu root and cranberry composition of the first aspect or the oral preparation of kudzu root and cranberry of the second aspect in the preparation of health food with an auxiliary protective effect against chemically induced liver injury, preferably in the preparation of health food with an auxiliary protective effect against alcoholic liver injury.

[0038] As a preferred embodiment of the present invention, the health food achieves an auxiliary protective effect against chemically induced liver injury, preferably alcoholic liver injury, by increasing the activity of alcohol dehydrogenase and / or aldehyde dehydrogenase in the body.

[0039] As a preferred embodiment of the present invention, the health food achieves an auxiliary protective effect against chemically induced liver injury, preferably alcoholic liver injury, by reducing the content of malondialdehyde in liver tissue and increasing the content of reduced glutathione.

[0040] As a preferred embodiment of the present invention, the health food achieves an auxiliary protective effect against chemically induced liver injury, preferably alcoholic liver injury, by adjusting the triglyceride content in liver tissue.

[0041] Fifthly, the present invention provides the use of the kudzu root and cranberry composition of the first aspect or the oral preparation of kudzu root and cranberry of the second aspect in the preparation of a medicine that has an effect on relieving hangovers and / or protecting the liver.

[0042] As a preferred embodiment of the present invention, the drug accelerates the metabolism of alcohol in the body by increasing the activity of alcohol dehydrogenase and / or acetaldehyde dehydrogenase, thereby achieving the effects of sobering up and further protecting liver tissue.

[0043] As a preferred embodiment of the present invention, the drug reduces liver tissue damage and promotes repair by decreasing the content of malondialdehyde in liver tissue and increasing the content of reduced glutathione, thereby achieving a protective effect on the liver.

[0044] As a preferred embodiment of the present invention, the drug prevents the accumulation of triglycerides in liver cells by controlling the content of triglycerides in liver tissue, thereby avoiding the formation of alcoholic fatty liver and achieving the effect of protecting the liver.

[0045] Compared with existing technologies, the composition provided by this invention combines traditional Chinese medicine compatibility theory with modern pharmacological research results. It uses a variety of Chinese herbal extracts in a specific ratio, allowing the components to complement and enhance each other in their mechanism of action. This results in an overall efficacy of the composition that is far greater than the simple sum of the individual effects of the components. The raw materials selected for this invention are all nationally approved medicinal and edible substances or new food ingredients, ensuring high safety, low toxicity and side effects. Furthermore, the raw materials are widely available, possessing significant market value and development prospects. Detailed Implementation

[0046] The present invention provides the following embodiments to further illustrate various aspects of the invention. These embodiments are non-limiting and should not be construed as limiting any aspect of the invention. The scope of protection of the present invention is limited only by the claims. Various modifications and improvements can be made to various aspects of the present invention by those skilled in the art without departing from the scope of the claims, and these modifications and improvements also fall within the scope of protection of the present invention.

[0047] Additionally, it should be noted that, unless otherwise specified, all materials and reagents used in the following embodiments are commonly used in the art and can be obtained through conventional commercial means; all methods used are conventional methods known to those skilled in the art.

[0048] Example The following are ingredients used in the composition of the experimental examples: The extract of Hovenia dulcis was prepared by the following method: Hovenia dulcis purchased from Tongrentang was boiled in water three times for three hours each time. The total amount of water used for extraction was equivalent to 20 times the mass of the Chinese herb. The extract was collected, centrifuged and filtered, and the filtrate was collected and spray-dried to obtain the final product.

[0049] The kudzu root extract was prepared by the following method: Kudzu root purchased from Tongrentang was boiled in water three times for three hours each time. The total amount of water used for extraction was equivalent to 20 times the mass of the Chinese herbal medicine. The extract was collected, centrifuged and filtered, and the filtrate was collected and spray-dried to obtain the final product.

[0050] The Poria cocos extract was prepared by the following method: Poria cocos purchased from Tongrentang was boiled in water three times for three hours each time. The total amount of water used for extraction was equivalent to 20 times the mass of the Chinese herbal medicine. The extract was collected, centrifuged and filtered, and the filtrate was collected and spray-dried to obtain the final product.

[0051] The licorice extract was prepared by the following method: licorice purchased from Tongrentang was boiled in water three times for three hours each time. The total amount of water used for extraction was equivalent to 20 times the mass of the licorice. The extract was collected, centrifuged and filtered, and the filtrate was collected and spray-dried to obtain the final product.

[0052] Astragalus extract was prepared by the following method: Astragalus purchased from Tongrentang was boiled in water three times for three hours each time. The total amount of water used for extraction was equivalent to 20 times the mass of the Chinese herb. The extract was collected, centrifuged and filtered, and the filtrate was collected and spray-dried to obtain the extract.

[0053] The yam extract was prepared by the following method: Take Chinese yam purchased from Tongrentang, add water and boil to extract 3 times, 3 hours each time. The total amount of water used for extraction is equivalent to 20 times the mass of the Chinese yam. Collect the extract, centrifuge and filter, collect the filtrate, and spray dry to obtain the extract.

[0054] Hawthorn extract was prepared by the following method: Hawthorn purchased from Tongrentang was boiled in water three times for three hours each time. The total amount of water used for extraction was equivalent to 20 times the weight of the Chinese herbal medicine. The extract was collected, centrifuged and filtered, and the filtrate was collected and spray-dried to obtain the final product.

[0055] The tangerine peel extract was prepared by the following method: Tangerine peel purchased from Tongrentang was boiled in water three times for three hours each time. The total amount of water used for extraction was equivalent to 20 times the mass of the tangerine peel. The extract was collected, centrifuged and filtered, and the filtrate was collected and spray-dried to obtain the final product.

[0056] The corn oligopeptide powder was purchased from Fufeng Sinote Biotechnology Co., Ltd.

[0057] Vitamin C was purchased from Northeast Pharmaceutical Co., Ltd.

[0058] Example 1 This embodiment provides a hangover relief and liver protection composition, which is composed of the following components: 42g of Hovenia dulcis extract, 42g of a mixed extract obtained by mixing cranberry, wolfberry and chrysanthemum in a mass ratio of 2:1:1, 38g of kudzu root extract, 13g of Poria cocos extract, 13g of licorice extract, 6g of Astragalus membranaceus extract, 9g of Dioscorea opposita extract, 4.5g of hawthorn extract, 3.5g of tangerine peel extract, 3.5g of corn oligopeptide powder, and 0.08g of vitamin C; The mixed extract was prepared by the following method: raw materials cranberry, wolfberry and chrysanthemum purchased from Tongrentang were mixed in a mass ratio of 2:1:1, water was added and boiled for extraction 3 times, 3 hours each time. The total amount of water used for extraction was equivalent to 20 times the total mass of the three ingredients. The extract was collected, centrifuged and filtered, the filtrate was collected and spray-dried to obtain the final product.

[0059] Example 2 This embodiment provides a hangover relief and liver protection composition, which is composed of the following components: 40g of Hovenia dulcis extract, 40g of a mixed extract obtained by mixing cranberry, wolfberry and chrysanthemum in a mass ratio of 1:2:1, 35g of kudzu root extract, 10g of Poria cocos extract, 10g of licorice extract, 8g of Astragalus membranaceus extract, 10g of Dioscorea opposita extract, 5g of hawthorn extract, 4g of tangerine peel extract, 4g of corn oligopeptide powder, and 0.1g of vitamin C; The mixed extract was prepared by the following method: raw materials cranberry, wolfberry and chrysanthemum purchased from Tongrentang were mixed in a mass ratio of 1:2:1, water was added and boiled for extraction 3 times, 3 hours each time. The total amount of water used for extraction was equivalent to 20 times the total mass of the three ingredients. The extract was collected, centrifuged and filtered, the filtrate was collected and spray-dried to obtain the final product.

[0060] Example 3 This embodiment provides a hangover relief and liver protection composition, which is composed of the following components: 45g of Hovenia dulcis extract, 45g of a mixed extract obtained by mixing cranberry, wolfberry and chrysanthemum in a mass ratio of 1:1:1, 40g of kudzu root extract, 15g of Poria cocos extract, 15g of licorice extract, 5g of Astragalus membranaceus extract, 8g of yam extract, 4g of hawthorn extract, 3g of tangerine peel extract, 3g of corn oligopeptide powder, and 0.05g of vitamin C; The mixed extract was prepared by the following method: raw materials cranberry, wolfberry and chrysanthemum purchased from Tongrentang were mixed in a mass ratio of 1:1:1, water was added and boiled for extraction 3 times, 3 hours each time. The total amount of water used for extraction was equivalent to 20 times the total mass of the three ingredients. The extract was collected, centrifuged and filtered, the filtrate was collected and spray-dried to obtain the final product.

[0061] Example 4 This embodiment provides a hangover relief and liver protection composition. The only difference from Example 1 is that the mixed extract does not contain chrysanthemum and is obtained by mixing and extracting cranberries and goji berries in a mass ratio of 1:1.

[0062] Example 5 This embodiment provides a composition for relieving hangovers and protecting the liver. The only difference from Example 1 is that the "adjuvant" in the composition does not contain astragalus extract, yam extract, hawthorn extract, tangerine peel extract, or corn oligopeptide powder.

[0063] Example 6 This embodiment provides a hangover-relieving and liver-protecting compressed candy, prepared by direct compression. The specific steps are as follows: the composition provided in Example 1 and all excipients are passed through an 80-mesh sieve and vacuum dried at 60°C for 2 hours; the composition, maltodextrin, xylitol, citric acid, and sodium carboxymethyl starch are added to a three-dimensional mixer and mixed for 15 minutes; hydroxypropyl cellulose is premixed manually for 5 minutes and then added to a general mixer for 5 minutes; the mixer is stopped and cooled to ≤30°C; sieved magnesium stearate and micronized silica gel are added and mixed for 3 minutes; a rotary tablet press is used for tableting, with a main pressure of 20 kN, a rotation speed of 30 rpm, a tablet weight of 1.0 g, a diameter of 20 mm, and a target hardness of 5-6 kgf.

[0064] Comparative Example 1 This comparative example provides a composition that differs from Example 1 of the present invention in that all purchased raw materials are mixed, water is added and boiled for extraction three times, each time for 3 hours, and the total amount of water used for extraction is equivalent to 20 times the mass of the Chinese medicine. The extract is collected, centrifuged and filtered, the filtrate is collected, and spray-dried to obtain the composition.

[0065] Comparative Example 2 This comparative example provides a composition that differs from Example 1 of the present invention in that cranberry, wolfberry, and chrysanthemum are extracted separately. Specifically, the medicinal materials are taken, boiled in water three times for three hours each time, and the total amount of water used for extraction is equivalent to 20 times the mass of the Chinese medicine. The extract is collected, centrifuged and filtered, the filtrate is collected, spray-dried, and the three extracts are mixed with other extracts to obtain the composition.

[0066] Experimental Example: Animal Models and Activity Detection When the body ingests large amounts of ethanol, alcohol dehydrogenase catalyzes a large amount of dehydrogenase, leading to impaired tricarboxylic acid cycle and weakened fatty acid oxidation, thus affecting lipid metabolism and causing fat deposition in liver cells. Simultaneously, ethanol can activate oxygen molecules, producing oxygen free radicals that cause lipid peroxidation of liver cell membranes and depletion of reduced glutathione in the body.

[0067] 1. Establishment of a mouse model of alcoholic liver injury The experiment consisted of nine experimental groups: Examples 1-5, Comparative Examples 1-2, a blank control group, and a model control group, with ten animals in each group. The test samples (i.e., Examples 1-5 and Comparative Examples 1-2, all at a concentration of 20 mg / mL) were administered orally via gavage daily at a dose of 12 mL / kg BW. The blank control group and the model control group received distilled water. The test samples were administered the drugs for 30 consecutive days. At the end of the test sample administration period, the model control group and each sample group were administered 12 mL / kg BW of 50% ethanol via gavage once, while the blank control group received distilled water. The animals were then sacrificed after a 16-hour fast, and various indicators were measured.

[0068] 2. Detection of ADH and ALDH enzyme activities in liver tissue The alcohol dehydrogenase assay kit (A083-2-1) and aldehyde dehydrogenase assay kit (A075-1-1) purchased from Nanjing Jiancheng Biotechnology Research Institute were used to detect the liver tissue homogenate supernatant samples of all test mice according to the instructions of the kits. The average ADH and ALDH activities of each group were obtained, and the results are shown in Table 1.

[0069] Table 1: ADH / ALDH activity detection results (n=10)

[0070] As shown in Table 1, the composition provided in the embodiments of the present invention can increase the activity of alcohol dehydrogenase and acetaldehyde dehydrogenase, thereby facilitating the rapid metabolism of alcohol.

[0071] 3. Detection of malondialdehyde content in liver tissue The MDA detection kit (A003-1) purchased from Nanjing Jiancheng Biotechnology Institute was used to detect the supernatant of liver tissue homogenate samples from all mice to be tested in accordance with the instructions of the kit. The average MDA content of each group was obtained, and the results are shown in Table 2.

[0072] Table 2: MDA Detection Results (n=10)

[0073] As shown in Table 2, the composition provided in the embodiments of the present invention can significantly reduce the MDA content in liver tissue and play a protective role in liver tissue.

[0074] 4. Detection of reduced glutathione content in liver tissue The micro-reduced glutathione (GSH) assay kit (A006-2-1) purchased from Nanjing Jiancheng Bioengineering Institute was used to detect the supernatant of liver tissue homogenate samples from all mice to be tested, and the average GSH content of each group was obtained according to the instructions of the kit. The results are shown in Table 3.

[0075] Table 3: GSH content detection results (n=10)

[0076] As shown in Table 3, the composition provided in the embodiments of the present invention can significantly increase the GSH content in liver tissue and play a protective role in liver tissue.

[0077] 5. Detection of triglyceride content in liver tissue The triglyceride (TG) content was detected using a triglyceride assay kit (A110-1-1) purchased from Nanjing Jiancheng Biotechnology Research Institute and an automated biochemical analyzer (Boke Biotechnology, BK-280). The average TG content of each group was obtained, and the results are shown in Table 4.

[0078] Table 4: TG content detection results (n=10)

[0079] As shown in Table 4, the composition provided in the embodiments of the present invention can significantly reduce the triglyceride content in liver tissue.

[0080] Based on the above experimental results, the composition provided by this invention can effectively increase the content of alcohol dehydrogenase and aldehyde dehydrogenase in hepatocytes, thereby exerting a hangover-relieving effect; it can effectively reduce the MDA content in tissues and increase the GSH content, preventing hepatocyte damage and thus protecting the liver; it can also effectively reduce the triglyceride content in hepatocytes, preventing fat accumulation in hepatocytes and thus preventing the occurrence of fatty liver, thereby exerting a comprehensive effect to achieve the hangover-relieving and liver-protecting effects.

[0081] Unless otherwise stated, the technical terms used herein have the same meanings as commonly understood by one of ordinary skill in the art. This invention may also be practiced using any methods and materials similar to or equivalent to those described herein. While specific embodiments and preferred methods and materials are described herein, they do not impose any limitation on the invention.

Claims

1. A kudzu root and cranberry composition, characterized in that, It contains the following ingredients in parts by weight: 30-50 parts of Hovenia dulcis extract, 30-50 parts of mixed extract, 20-40 parts of Pueraria lobata extract, 10-20 parts of Poria cocos extract, 10-20 parts of Glycyrrhiza uralensis extract, and 0.05-0.1 parts of Vitamin C. The mixed extract is obtained by first mixing the raw materials cranberries and goji berries in a mass ratio of (1~2):(1~2) and then extracting them; The extracts were obtained by extraction, filtration and drying using water as a solvent.

2. The composition according to claim 1, characterized in that, The raw materials of the mixed extract also include chrysanthemum; the raw materials used in the mixed extract are cranberry, wolfberry and chrysanthemum mixed in a mass ratio of (1~2):(1~2):(1~2).

3. The composition according to claim 2, characterized in that, The raw materials used in the mixed extract are cranberries, goji berries, and chrysanthemums mixed in a mass ratio of 2:(1~1.5):(1~1.5).

4. The composition according to any one of claims 1 to 3, characterized in that, The composition also contains 5-10 parts of Astragalus membranaceus extract, 5-10 parts of Dioscorea opposita extract, 3-6 parts of Crataegus pinnatifida extract, 3-6 parts of Citrus reticulata extract, and 3-6 parts of corn oligopeptide powder.

5. The composition according to any one of claims 1 to 4, characterized in that, It contains the following ingredients in parts by weight: 40-45 parts of Hovenia dulcis extract, 40-45 parts of a mixed extract obtained by mixing cranberry, wolfberry and chrysanthemum in a mass ratio of 2:(1-1.5):(1-1.5), 35-40 parts of kudzu root extract, 10-15 parts of Poria cocos extract, 10-15 parts of licorice extract, 5-8 parts of Astragalus membranaceus extract, 8-10 parts of Dioscorea opposita extract, 4-5 parts of hawthorn extract, 3-4 parts of tangerine peel extract, 3-4 parts of corn oligopeptide powder, and 0.05-0.1 parts of vitamin C.

6. An oral preparation of kudzu root and cranberry, characterized in that, The composition comprises the kudzu root and cranberry composition according to any one of claims 1 to 5, and excipients; The oral preparation is a tablet, preferably a regular tablet, lozenge, chewable tablet, dispersible tablet or effervescent tablet; Alternatively, the oral preparation may be a capsule, preferably a hard capsule or a soft capsule; Alternatively, the oral preparation is a granule, preferably a soluble granule, a suspension granule, or an effervescent granule. Alternatively, the oral preparation may be a powder; Alternatively, the oral preparation may be a paste; Alternatively, the oral preparation is a candy, preferably hard candy, hard filled candy, cream candy, gel candy, gum-based candy, aerated candy or compressed candy; Alternatively, the oral formulation may be a liquid formulation, preferably a syrup, oral liquid, or beverage.

7. The oral formulation according to claim 6, characterized in that, It is a compressed candy, and the sweetener used is xylitol.

8. A food product, characterized in that, The composition comprising any one of claims 1 to 5 or the oral formulation of kudzu root and cranberry as described in claim 6 or 7.

9. The use of the kudzu root and cranberry composition according to any one of claims 1 to 5, or the kudzu root and cranberry oral preparation according to claim 6 or 7, in the preparation of health foods with an adjunctive protective effect against chemically induced liver injury.

10. The use of the kudzu root and cranberry composition according to any one of claims 1 to 5, or the kudzu root and cranberry oral preparation according to claim 6 or 7, in the preparation of a medicine having an effect on relieving hangovers and / or protecting the liver.

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