Composition containing coenzyme q10 for maintaining blood pressure at a healthy level and method for preparing the same

Abstract

The present application belongs to the technical field of health care products, and particularly relates to a composition containing coenzyme Q10 and helpful to maintaining a healthy level of blood pressure and a preparation method thereof. The present application discloses a composition containing coenzyme Q10 and helpful to maintaining a healthy level of blood pressure, which comprises the following ingredients in parts by weight: 12-17 parts of traditional Chinese medicine extract, and 3-5 parts of coenzyme Q10; the traditional Chinese medicine extract is prepared by first extracting salvia miltiorrhiza, panax notoginseng, ginkgo leaves, hawthorn and semen cassiae with water to obtain an extract, and then fermenting the extract with microorganisms. The composition prepared by the present application has excellent efficacy in maintaining a healthy level of blood pressure, and when applied to an oral liquid helpful to maintaining a healthy level of blood pressure, no toxicity problem is verified through a toxicological test, and the composition can be used as a safe health care product.

Description

Technical Field

[0001] This invention belongs to the field of health product technology, specifically relating to a composition containing coenzyme Q10 that helps maintain healthy blood pressure levels and its preparation method. Background Technology

[0002] Hypertension is a chronic disease characterized by elevated systolic or diastolic blood pressure. Its complications, such as stroke, myocardial infarction, heart failure, and chronic kidney disease, coupled with the increasing prevalence and the trend of affecting younger people, place a heavy burden on families and society and have become a major public health problem worldwide.

[0003] Coenzyme Q10, also known as ubiquinone 10, is a vitamin-like compound that is a fat-soluble organic quinone widely distributed in organisms. It has excellent therapeutic effects on diseases such as heart disease, hypertension, cerebrovascular disorders, scurvy, and viral hepatitis. At the same time, it does not interact with any other drugs and is non-toxic and has no side effects. Therefore, coenzyme Q10 plays an irreplaceable role and has broad application prospects in human health care, delaying aging, and improving the body's immunity.

[0004] Hypertension, as one of the most common chronic diseases, can cause significant side effects and damage to the body when medication is taken long-term. Therefore, it is imperative to develop a composition containing coenzyme Q10 to maintain healthy blood pressure levels, prevent hypertension, and improve the quality of life for people with hypertension. Summary of the Invention

[0005] In view of the above-mentioned defects or improvement needs of the prior art, the present invention provides a composition containing coenzyme Q10 that helps maintain healthy blood pressure levels and a method for preparing the same. The composition of the present invention can effectively maintain healthy blood pressure levels and improve the quality of life of people with hypertension.

[0006] To achieve the above objectives, the present invention adopts the following technical solution: In a first aspect, the present invention provides a composition containing coenzyme Q10 that helps maintain healthy blood pressure levels, the composition comprising the following components in parts by weight: 12-17 parts of traditional Chinese medicine extract and 3-5 parts of coenzyme Q10; The preparation method of the traditional Chinese medicine extract includes the following steps: W1. The dried Chinese herbal raw materials are pulverized separately, passed through a 100-mesh sieve, and mixed to obtain a mixture; wherein, the Chinese herbal raw materials include Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and cassia seed, and the Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and cassia seed are mixed in a weight ratio of 4-8:2-5:2-7:11-16:10-15; W2. Add 9-13 times the weight of deionized water to the mixture, soak at room temperature for 30-60 minutes, heat and extract at 90-100℃ for 50-70 minutes, filter and collect the filtrate, sterilize, and obtain the water extract. W3. After the aqueous extract has cooled to room temperature, it is first inoculated with Staphylococcus xylose seed liquid for aerobic fermentation, and then inoculated with Lactobacillus acidophilus seed liquid for anaerobic fermentation. After fermentation, the extract is centrifuged, and the supernatant is filtered to remove bacteria, thus obtaining the Chinese herbal extract. The preservation number of Staphylococcus xylose is CCTCC NO: M 2023516, and the preservation number of Lactobacillus acidophilus is CGMCC No. 22239.

[0007] Tanshinone in Danshen exerts its vasodilatory effect by promoting the release of vasodilatory factors, partially opening potassium channels, and inhibiting calcium channels, thereby improving hypertension.

[0008] Panax notoginseng is a perennial herbaceous plant. Saponins are the main physiologically active components in Panax notoginseng, which have the effects of dilating blood vessels and lowering blood pressure, with particularly excellent effects in lowering diastolic blood pressure.

[0009] Ginkgo leaves contain flavonoids, terpene lactones, organic acids, polysaccharides, and other components, which can promote mineral absorption, regulate the nervous system, and have antioxidant, blood sugar lowering, blood pressure lowering, blood lipid lowering, cholesterol lowering, liver protection, and antibacterial effects.

[0010] Hawthorn primarily aids digestion, strengthens the spleen, promotes qi circulation, dispels blood stasis, eliminates turbidity, and lowers lipids. It can dilate peripheral blood vessels to lower blood pressure, enhance the resistance of vascular endothelial cells to damage, and regulate and promote lipid decomposition.

[0011] Cassia seeds mainly contain pharmacological components such as rhein, emodin methyl ether, cassia seed extract, and cassia seed extract, which have effects such as lowering blood pressure, regulating blood lipids, protecting the liver and improving eyesight, antibacterial, and antioxidant properties.

[0012] Preferably, the composition comprises the following components in parts by weight: 15 parts of traditional Chinese medicine extract and 4 parts of coenzyme Q10.

[0013] Preferably, in step W1, danshen, sanqi, ginkgo leaves, hawthorn and cassia seeds are mixed in a weight ratio of 6:3:4:13:12.

[0014] Preferably, in step W3, the inoculation amount of Staphylococcus xylose seed solution is 1% v / v-2% v / v of the water extract, and the inoculation amount of Lactobacillus acidophilus seed solution is 1.5% v / v-3% v / v of the water extract.

[0015] More preferably, in step W3, the inoculation amount of Staphylococcus xylose seed solution is 1.5% v / v of the water extract, and the inoculation amount of Lactobacillus acidophilus seed solution is 2.3% v / v of the water extract.

[0016] Preferably, in step W3, the viable count of both the Staphylococcus xylose seed solution and the Lactobacillus acidophilus seed solution is 1.0 × 10⁻⁶. 8 CFU / mL.

[0017] Preferably, the aerobic fermentation in step W3 is as follows: at 35-38°C, sterile air with an aeration ratio of 0.2-0.5 vvm is introduced for aerobic fermentation for 24-36 hours.

[0018] Preferably, the anaerobic fermentation in step W3 is carried out at 35-38℃ under anaerobic conditions for 18-24 hours.

[0019] In a second aspect, the present invention provides the use of the composition described in the first aspect in the preparation of health products that help maintain healthy blood pressure levels.

[0020] Preferably, the dosage form of the health product is any one of tablets, hard capsules, soft capsules, ointments, granules, or powders.

[0021] The beneficial effects of this invention are: The composition provided by this invention is prepared by compounding a traditional Chinese medicine extract and coenzyme Q10. The traditional Chinese medicine extract uses Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and Cassia tora seeds as raw materials. The extract is first obtained by water extraction and then by microbial fermentation. The microbial fermentation adopts a method of first inoculating Staphylococcus xylose for aerobic fermentation and then inoculating Lactobacillus acidophilus for anaerobic fermentation. The two fermentation methods can cooperate and promote each other, thereby increasing the fermentation speed, shortening the fermentation time, providing richer nutrients, and synergistically enhancing the effect in maintaining healthy blood pressure levels. Under the premise of consistent total inoculum quantity, the fermentation method provided by this invention is more effective than other fermentation methods. This invention can further improve the fermentation effect by screening strains and optimizing the ratio of viable bacteria in each fermentation bacteria.

[0022] The composition prepared by this invention has excellent efficacy in maintaining healthy blood pressure levels. When applied to oral liquids that help maintain healthy blood pressure levels, it has been verified through toxicological tests that there are no toxic issues and it can be used as a safe health supplement. Detailed Implementation

[0023] To further illustrate the technical means and effects of the present invention in achieving the intended purpose, the following detailed description of the specific embodiments, structure, features and effects of the present invention, in conjunction with the composition, is provided below.

[0024] For the following compositions, unless otherwise specified, experimental methods are generally performed under standard conditions or as recommended by the manufacturer. Unless otherwise specified, all materials and reagents used are commercially available.

[0025] Some of the raw materials and their sources are as follows: The Salvia miltiorrhiza was purchased from Huaxia Ankang Pharmacy's Tmall flagship store; The Panax notoginseng was purchased from the Gening Pharmacy Tmall flagship store; The ginkgo leaves were purchased from Hongyitang's Tmall flagship store; The hawthorns were purchased from Jizhentang's Tmall flagship store; The cassia seeds were purchased from the Minnongren Tmall flagship store.

[0026] Chinese herbal extract ① Its preparation method includes the following steps: W1. Crush the dried Chinese medicinal materials separately, pass them through a 100-mesh sieve, and mix them together to obtain a mixture; W2. Add 9 times the weight of deionized water to the mixture, soak at room temperature for 60 minutes, heat and extract at 90°C for 70 minutes, filter and collect the filtrate, sterilize, and obtain the aqueous extract. W3. After the aqueous extract has cooled to room temperature, it is first inoculated with Staphylococcus xylose seed liquid for aerobic fermentation, and then inoculated with Lactobacillus acidophilus seed liquid for anaerobic fermentation. After fermentation, it is centrifuged, and the supernatant is filtered and sterilized to obtain the Chinese herbal extract. The traditional Chinese medicine raw materials in step W1 include Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and cassia seed. The Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and cassia seed are mixed in a weight ratio of 4:5:7:11:15. The sterilization temperature in step W2 is 100℃ and the time is 5 minutes. In step W3, the preservation number of *Staphylococcus xylose* is CCTCC NO: M 2023516, and the preservation number of *Lactobacillus acidophilus* is CGMCC No. 22239; the inoculation amount of the *Staphylococcus xylose* seed solution is 1% v / v of the water extract, and the inoculation amount of the *Lactobacillus acidophilus* seed solution is 3% v / v of the water extract; the viable count of both the *Staphylococcus xylose* seed solution and the *Lactobacillus acidophilus* seed solution is 1.0 × 10⁻⁶. 8 CFU / mL; the aerobic fermentation is carried out at 35°C with sterile air at an aeration ratio of 0.2vvm for 36 hours; the anaerobic fermentation is carried out at 35°C under anaerobic conditions for 24 hours; the centrifugation speed is 3000 rpm and the time is 25 min; the filtration sterilization is carried out using a 0.22 μm sterile filter membrane.

[0027] Traditional Chinese medicine extract ② Its preparation method includes the following steps: W1. Crush the dried Chinese medicinal materials separately, pass them through a 100-mesh sieve, and mix them together to obtain a mixture; W2. Add 11 times the weight of deionized water to the mixture, soak at room temperature for 45 minutes, heat and extract at 95°C for 60 minutes, filter and collect the filtrate, sterilize, and obtain the water extract. W3. After the aqueous extract has cooled to room temperature, it is first inoculated with Staphylococcus xylose seed liquid for aerobic fermentation, and then inoculated with Lactobacillus acidophilus seed liquid for anaerobic fermentation. After fermentation, it is centrifuged, and the supernatant is filtered and sterilized to obtain the Chinese herbal extract. The traditional Chinese medicine raw materials in step W1 include Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and cassia seed. The Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and cassia seed are mixed in a weight ratio of 6:3:4:13:12. The sterilization temperature in step W2 is 110℃ and the time is 3 minutes. In step W3, the preservation number of *Staphylococcus xylose* is CCTCC NO: M 2023516, and the preservation number of *Lactobacillus acidophilus* is CGMCC No. 22239; the inoculation amount of the *Staphylococcus xylose* seed culture is 1.5% v / v of the water extract, and the inoculation amount of the *Lactobacillus acidophilus* seed culture is 2.3% v / v of the water extract; the viable count of both *Staphylococcus xylose* and *Lactobacillus acidophilus* seed cultures is 1.0 × 10⁻⁶. 8 CFU / mL; the aerobic fermentation is carried out at 37°C with sterile air at an aeration ratio of 0.4 vvm for 30 h; the anaerobic fermentation is carried out at 37°C under anaerobic conditions for 20 h; the centrifugation speed is 4000 rpm and the time is 20 min; the filtration sterilization is carried out using a 0.22 μm sterile filter membrane.

[0028] Traditional Chinese medicine extract ③ Its preparation method includes the following steps: W1. Crush the dried Chinese medicinal materials separately, pass them through a 100-mesh sieve, and mix them together to obtain a mixture; W2. Add 13 times the weight of deionized water to the mixture, soak at room temperature for 30 minutes, heat at 100°C for 50 minutes, filter and collect the filtrate, sterilize, and obtain the water extract. W3. After the aqueous extract has cooled to room temperature, it is first inoculated with Staphylococcus xylose seed liquid for aerobic fermentation, and then inoculated with Lactobacillus acidophilus seed liquid for anaerobic fermentation. After fermentation, it is centrifuged, and the supernatant is filtered and sterilized to obtain the Chinese herbal extract. The traditional Chinese medicine raw materials in step W1 include Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and cassia seed. The Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and cassia seed are mixed in a weight ratio of 8:2:2:16:10. The sterilization temperature in step W2 is 120℃ and the time is 1 min; In step W3, the preservation number of *Staphylococcus xylose* is CCTCC NO: M 2023516, and the preservation number of *Lactobacillus acidophilus* is CGMCC No. 22239; the inoculation amount of the *Staphylococcus xylose* seed culture is 2% v / v of the water extract, and the inoculation amount of the *Lactobacillus acidophilus* seed culture is 1.5% v / v of the water extract; the viable count of both *Staphylococcus xylose* and *Lactobacillus acidophilus* seed cultures is 1.0 × 10⁻⁶. 8 CFU / mL; the aerobic fermentation is carried out at 38°C with sterile air at an aeration ratio of 0.5 vvm for 24 hours; the anaerobic fermentation is carried out at 38°C under anaerobic conditions for 18 hours; the centrifugation speed is 5000 rpm and the time is 15 min; the filtration sterilization is carried out using a 0.22 μm sterile filter membrane.

[0029] Traditional Chinese medicine extract ④ Compared with the traditional Chinese medicine extract ②, the only difference lies in the preparation step W3 of the traditional Chinese medicine extract ④: after the aqueous extract cools to room temperature, it is first inoculated with *Staphylococcus xylose* seed liquid for the first stage of aerobic fermentation, and then inoculated with *Lactobacillus acidophilus* seed liquid for the second stage of aerobic fermentation. After fermentation, it is centrifuged, the supernatant is collected, filtered and sterilized to obtain the traditional Chinese medicine extract; wherein, the preservation number of *Staphylococcus xylose* is CCTCC NO: M 2023516, the preservation number of *Lactobacillus acidophilus* is CGMCC No. 22239, the inoculation amount of *Staphylococcus xylose* seed liquid is 1.5% v / v of the aqueous extract, and the inoculation amount of *Lactobacillus acidophilus* seed liquid is 2.3% v / v of the aqueous extract; the viable count of both the *Staphylococcus xylose* seed liquid and the *Lactobacillus acidophilus* seed liquid is 1.0 × 10⁻⁶. 8 CFU / mL; the first stage of aerobic fermentation is carried out at 37°C with sterile air at an aeration ratio of 0.4 vvm for 30 h; the second stage of aerobic fermentation is carried out at 37°C with sterile air at an aeration ratio of 0.4 vvm for 20 h; the centrifugation speed is 4000 rpm and the time is 20 min; the filtration sterilization is carried out using a 0.22 μm sterile filter membrane; other steps and parameters are the same as those of the traditional Chinese medicine extract ②.

[0030] Chinese herbal extract ⑤ Compared with the traditional Chinese medicine extract ②, the only difference lies in the preparation step W3 of the traditional Chinese medicine extract ⑤: after the aqueous extract cools to room temperature, *Staphylococcus xylose* seed solution and *Lactobacillus acidophilus* seed solution are inoculated simultaneously. Aerobic fermentation is performed first, followed by anaerobic fermentation. After fermentation, the mixture is centrifuged, the supernatant is collected, filtered, and sterilized to obtain the traditional Chinese medicine extract. The *Staphylococcus xylose* has the preservation number CCTCC NO: M 2023516, and the *Lactobacillus acidophilus* has the preservation number CGMCC No. 22239. The inoculation amount of the *Staphylococcus xylose* seed solution is 1.5% v / v of the aqueous extract, and the inoculation amount of the *Lactobacillus acidophilus* seed solution is 2.3% v / v of the aqueous extract. The viable count of both the *Staphylococcus xylose* and *Lactobacillus acidophilus* seed solutions is 1.0 × 10⁻⁶. 8 CFU / mL; the aerobic fermentation was carried out at 37°C with sterile air at a ventilation ratio of 0.4 vvm for 30 h; the anaerobic fermentation was carried out at 37°C under anaerobic conditions for 20 h; the centrifugation speed was 4000 rpm and the time was 20 min; the filtration sterilization was carried out using a 0.22 μm sterile filter membrane; other steps and parameters were the same as those for the traditional Chinese medicine extract ②.

[0031] Traditional Chinese medicine extract ⑥ Compared with the traditional Chinese medicine extract ②, the only difference lies in the preparation step W3 of the traditional Chinese medicine extract ⑥: after the aqueous extract cools to room temperature, it is first inoculated with *Lactobacillus acidophilus* seed liquid for anaerobic fermentation, and then inoculated with *Staphylococcus xylose* seed liquid for aerobic fermentation. After fermentation, it is centrifuged, the supernatant is collected, filtered and sterilized to obtain the traditional Chinese medicine extract; wherein, the preservation number of *Staphylococcus xylose* is CCTCC NO: M 2023516, the preservation number of *Lactobacillus acidophilus* is CGMCC No. 22239, the inoculation amount of *Lactobacillus acidophilus* seed liquid is 2.3% v / v of the aqueous extract, and the inoculation amount of *Staphylococcus xylose* seed liquid is 1.5% v / v of the aqueous extract; the viable count of both the *Staphylococcus xylose* seed liquid and the *Lactobacillus acidophilus* seed liquid is 1.0 × 10⁻⁶. 8 CFU / mL; the anaerobic fermentation was carried out at 37℃ under anaerobic conditions for 20h; the aerobic fermentation was carried out at 37℃ with sterile air at an aeration ratio of 0.4 vvm for 30h; the centrifugation speed was 4000rpm and the time was 20min; the filtration sterilization was carried out using a 0.22μm sterile filter membrane; other steps and parameters were the same as those of the traditional Chinese medicine extract ②.

[0032] Chinese herbal extracts ⑦ Compared with the Chinese herbal extract ②, the only difference is that in the Chinese herbal extract ⑦, Staphylococcus aureus with accession number CGMCC No.28186 is used instead of Staphylococcus xylose with accession number CCTCC NO: M 2023516. All other steps and parameters are the same as those of the Chinese herbal extract ②.

[0033] Traditional Chinese medicine extract ⑧ Compared with the Chinese herbal extract ②, the difference is that in the Chinese herbal extract ⑧, Lactobacillus rhamnosus with preservation number CGMCC No.20110 is used instead of Lactobacillus acidophilus with preservation number CGMCC No.22239. All other steps and parameters are the same as those of the Chinese herbal extract ②.

[0034] Chinese herbal extracts ⑨ Compared with the Chinese herbal extract ②, the difference is that the inoculation amount of Staphylococcus xylose seed liquid in the Chinese herbal extract ⑨ is 0.3% v / v of the water extract, and the inoculation amount of Lactobacillus acidophilus seed liquid is 3.5% v / v of the water extract. All other steps and parameters are the same as those of the Chinese herbal extract ②.

[0035] Chinese herbal extract ⑩ Compared with the Chinese herbal extract ②, the difference is that the inoculation amount of Staphylococcus xylose seed liquid in the Chinese herbal extract ⑩ is 2.8% v / v of the water extract, and the inoculation amount of Lactobacillus acidophilus seed liquid is 1% v / v of the water extract. All other steps and parameters are the same as those of the Chinese herbal extract ②.

[0036] The component composition (parts by weight) of compositions 1-5 in this invention is shown in Table 1 below. Each component is weighed according to the formula amount, mixed and homogenized to obtain each composition.

[0037] Table 1. Components and weight parts of compositions 1-5 Group Traditional Chinese medicine extract number Traditional Chinese medicine extract (parts by weight) Coenzyme Q10 (parts by weight) Composition 1 ① 15 4 Composition 2 ② 15 4 Composition 3 ③ 15 4 Composition 4 ② 12 3 Composition 5 ② 17 5 Composition ① Compared with composition 2, the difference is that the traditional Chinese medicine extract ② is not added to composition ①, and the missing amount is made up with coenzyme Q10. All other steps and parameters are the same as those of composition 2.

[0038] Composition ② Compared with composition 2, the difference is that coenzyme Q10 is not added in composition 2, and the missing amount is made up by the traditional Chinese medicine extract ②. All other steps and parameters are the same as those of composition 2.

[0039] Composition ③ Compared with composition 2, the difference is that in composition 3, the traditional Chinese medicine extract ④ is used to replace the traditional Chinese medicine extract ② in an equal amount, while the other steps and parameters are the same as those in composition 2.

[0040] Composition ④ Compared with composition 2, the difference is that in composition ④, the traditional Chinese medicine extract ⑤ is used to replace the traditional Chinese medicine extract ② in an equal amount, while the other steps and parameters are the same as those of composition 2.

[0041] Composition ⑤ Compared with composition 2, the difference is that in composition ⑤, the traditional Chinese medicine extract ⑥ is used to replace the traditional Chinese medicine extract ② in an equal amount, while the other steps and parameters are the same as those of composition 2.

[0042] Composition ⑥ Compared with composition 2, the difference is that in composition ⑥, the traditional Chinese medicine extract ⑦ is used to replace the traditional Chinese medicine extract ② in an equal amount, while the other steps and parameters are the same as those of composition 2.

[0043] Composition ⑦ Compared with composition 2, the difference is that in composition ⑦, the traditional Chinese medicine extract ⑧ is used to replace the traditional Chinese medicine extract ② in an equal amount, while the other steps and parameters are the same as those of composition 2.

[0044] Composition ⑧ Compared with composition 2, the difference is that composition ⑧ uses a traditional Chinese medicine extract ⑨ to replace traditional Chinese medicine extract ② in an equal amount, while the other steps and parameters are the same as those of composition 2.

[0045] Composition 9 Compared with composition 2, the difference is that composition 9 uses Chinese herbal extract 10 to replace Chinese herbal extract 2 in an equal amount, while the other steps and parameters are the same as composition 2.

[0046] Test Example 1: Composition for Maintaining Healthy Blood Pressure Levels This test case follows the "Test Method for Maintaining Healthy Blood Pressure Levels" in the "Methods for Functional Testing and Evaluation of Health Foods" (2023 Edition) to test the composition and the composition prepared from the composition for maintaining healthy blood pressure levels.

[0047] Experimental setup: A normal control group, a hypertension model group, a positive control group, and an experimental group (compositions 1-5 and ①-⑨, respectively) were set up, with 8 rats in each group. They were fed a standard diet with free access to food and water. The experimental period was 30 days. The normal control group consisted of SD rats without hypertension characteristics and with stable blood pressure, half male and half female, aged 3-4 months, weighing 200-220g. The hypertension model group, positive control group, and experimental group consisted of spontaneously hypertensive rats (SHR), half male and half female, aged 3-4 months, weighing 200-220g. The normal control group and the hypertension model group were administered an equal volume of physiological saline by gavage at a dose of 10 mg / kg. The positive control group was administered captopril by gavage at a dose of 10 mg / kg. The experimental groups were administered compositions 1-5 and compositions ①-⑨ by gavage at a dose of 10 mg / kg. Blood pressure measurement: Systolic and diastolic blood pressure of the rat tail artery were measured. Data processing: All measurement data are expressed as mean ± standard deviation. Statistical analysis was performed using t-tests. P < 0.05 was considered statistically significant. The specific results are shown in Table 2.

[0048] Table 2 Test Results of Test Example 1

[0049] Note:" ▲ "This indicates that, compared with the control group of the hypertension model, each group showed a p-value < 0.05 after the experiment." # "" indicates that the systolic and diastolic blood pressures of each group after the test were compared with those before the test, and P < 0.05.

[0050] Test Example 2: Angiotensin-converting enzyme inhibition test The renin-angiotensin-aldosterone system plays a crucial role in the molecular mechanism of blood pressure regulation. Angiotensin-converting enzyme (ACE), as the core component of this system, can catalyze the conversion of angiotensin I into angiotensin II, which has a strong vasoconstrictive effect. Therefore, inhibiting ACE activity can effectively lower blood pressure. In this test case, the antihypertensive effect was evaluated by detecting the ACE inhibition rate of compositions 1-5 and compositions ①-⑨.

[0051] Sample preparation: Dilute compositions 1-5 and compositions ①-⑨ to a concentration of 1 mg / mL in 0.1 mol / L sodium borate buffer (pH=8.3, containing 0.3 M sodium chloride); The experiment used 96-well microplates. For the sample group, 10 μL of 0.1 U / mL ACE solution (prepared with 0.1 mol / L sodium borate buffer) and 20 μL of sample solution were added to each well. For the control group, 10 μL of 0.1 U / mL ACE solution and 20 μL of ultrapure water were added to each well. After mixing, the mixture was incubated at 37°C for 10 min. Then, 50 μL of ACE solution was added to each well of both the sample and control groups. A 0.8 mmol / L FAPPGG (N-[3-(2-furanyl)acryloyl]-L-phenylalanyl-glycyl-glycine) solution was used to immediately measure the initial absorbance (a1) of the control group and the initial absorbance (b1) of the sample group at a wavelength of 340 nm. After incubation at 37 °C for 30 min, the absorbance (a2) of the control group and the absorbance (b2) of the sample group at a wavelength of 340 nm were measured again. Each group had three replicates, and the ACE inhibition rate was calculated according to the following formula. The data are presented as average values, and the specific results are shown in Table 3. ; Where: a1 is the initial absorbance value of the control group; a2 is the absorbance value of the control group after 30 min of incubation; b1 is the initial absorbance value of the sample group; b2 is the absorbance value of the sample group after 30 min of incubation. Table 3 ACE inhibition rate data Group ACE inhibition rate (%) Composition 1 70.25 Composition 2 75.62 Composition 3 69.37 Composition 4 73.58 Composition 5 72.14 Composition ① 47.21 Composition ② 51.49 Composition ③ 58.03 Composition ④ 59.86 Composition ⑤ 57.34 Composition ⑥ 63.40 Composition ⑦ 62.19 Composition ⑧ 67.23 Composition 9 65.92 Compared with composition 2, compositions ①-② do not contain either traditional Chinese medicine extract ② or coenzyme Q10, while compositions ③-⑨ involve changes in the preparation process of the traditional Chinese medicine extract.

[0052] The data on systolic blood pressure, diastolic blood pressure, and ACE inhibition rate of compositions 1-5 before and after the test in Tables 2-3 show that when the weight ratio of traditional Chinese medicine extract to coenzyme Q10 is (12-17):(3-5), the composition has a better effect on maintaining healthy blood pressure levels. Combining the data of compositions 2 and 4-5 in Tables 2-3, the composition has the best effect when the weight ratio of traditional Chinese medicine extract to coenzyme Q10 is 15:4.

[0053] As can be seen from the data of composition 2 and compositions ①-② in Tables 2-3, the traditional Chinese medicine extract and coenzyme Q10 can synergistically improve the composition's ability to maintain a healthy blood pressure level.

[0054] As can be seen from the data of composition 2 and compositions ③-⑨ in Tables 2-3, different fermentation methods, different fermentation strains, and different inoculation ratios will all affect the fermentation effect, thereby affecting the effect of the composition on maintaining a healthy blood pressure level.

[0055] Application example: An oral liquid for maintaining healthy blood pressure levels Compositions 1-5 were added to the oral liquid to obtain the oral liquids of Application Examples 1-5.

[0056] The oral liquid for maintaining healthy blood pressure levels comprises the following ingredients by weight: 46 parts of the composition, 25 parts of sugar alcohol, and 0.5 parts of steviol glycosides.

[0057] The preparation method of the oral liquid for maintaining healthy blood pressure includes the following steps: weigh each raw material according to the formula, mix them evenly, add 42 times the weight of drinking water, sterilize at 121°C for 15 minutes, and obtain the oral liquid.

[0058] Blank application example Compared with Application Example 1, the difference is that no composition is added to the oral liquid in the blank application example, and an equal amount of drinking water is used instead of the composition. The preparation method is the same as that of Application Example 1.

[0059] Test Example 3: Toxicology Test Seventy SPF-grade adult healthy female ICR rats, weighing 18-22g, were divided into a control group and an experimental group, with 10 rats in each group. The experimental group was administered 0.5mL / 20g of the oral solution prepared in Examples 1-5 and the blank application example by gavage daily, while the blank control group was administered the same amount of drinking water by gavage daily. The gavage was performed for 2 days. The condition of the rats was observed after each gavage, and the number of dead rats was counted. The condition of rats that did not die after 48 hours was observed for 2 weeks. The results showed that all members of both the blank control group and the experimental group survived and were in good condition, indicating that the composition prepared in this application has no toxicity issues when applied to oral liquids and can be used as a safe health product.

[0060] The above description is merely a preferred composition of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with preferred compositions, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent compositions without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above compositions based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A composition containing coenzyme Q10 that helps maintain healthy blood pressure levels, characterized in that, The composition comprises the following components in parts by weight: 12-17 parts of traditional Chinese medicine extract and 3-5 parts of coenzyme Q10; The preparation method of the traditional Chinese medicine extract includes the following steps: W1. The dried Chinese herbal raw materials are pulverized separately, passed through a 100-mesh sieve, and mixed to obtain a mixture; wherein, the Chinese herbal raw materials include Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and cassia seed, and the Salvia miltiorrhiza, Panax notoginseng, Ginkgo biloba leaves, hawthorn, and cassia seed are mixed in a weight ratio of 4-8:2-5:2-7:11-16:10-15; W2. Add 9-13 times the weight of deionized water to the mixture, soak at room temperature for 30-60 minutes, heat and extract at 90-100℃ for 50-70 minutes, filter and collect the filtrate, sterilize, and obtain the water extract. W3. After the aqueous extract has cooled to room temperature, it is first inoculated with Staphylococcus xylose seed liquid for aerobic fermentation, and then inoculated with Lactobacillus acidophilus seed liquid for anaerobic fermentation. After fermentation, the extract is centrifuged, and the supernatant is filtered to remove bacteria, thus obtaining the Chinese herbal extract. The preservation number of Staphylococcus xylose is CCTCC NO: M 2023516, and the preservation number of Lactobacillus acidophilus is CGMCC No. 22239.

2. The composition according to claim 1, characterized in that, The composition comprises the following components in parts by weight: 15 parts of traditional Chinese medicine extract and 4 parts of coenzyme Q10.

3. The composition according to claim 1, characterized in that, In step W1, danshen, sanqi, ginkgo leaves, hawthorn and cassia seeds are mixed in a weight ratio of 6:3:4:13:

12.

4. The composition according to claim 1, characterized in that, In step W3, the inoculation amount of Staphylococcus xylose seed solution is 1% v / v-2% v / v of the water extract, and the inoculation amount of Lactobacillus acidophilus seed solution is 1.5% v / v-3% v / v of the water extract.

5. The composition according to claim 4, characterized in that, In step W3, the inoculation amount of Staphylococcus xylose seed solution is 1.5% v / v of the water extract, and the inoculation amount of Lactobacillus acidophilus seed solution is 2.3% v / v of the water extract.

6. The composition according to claim 1, characterized in that, In step W3, the viable cell counts of both the Staphylococcus xylose seed solution and the Lactobacillus acidophilus seed solution were 1.0 × 10⁻⁶. 8 CFU / mL.

7. The composition according to claim 1, characterized in that, The aerobic fermentation in step W3 is as follows: at 35-38℃, sterile air with an aeration ratio of 0.2-0.5 vvm is introduced for aerobic fermentation for 24-36 hours.

8. The composition according to claim 1, characterized in that, The anaerobic fermentation in step W3 is carried out at 35-38℃ under anaerobic conditions for 18-24 hours.

9. Use of the composition of any one of claims 1-8 in the preparation of a health supplement that helps maintain healthy blood pressure levels.

10. The application as described in claim 9, characterized in that, The dosage form of the health product is any one of tablets, hard capsules, soft capsules, ointments, granules, or powders.

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