A plant-derived composition capable of reducing uric acid and a preparation method and application thereof

Abstract

The present application relates to the technical field of plant extract application, and discloses a plant source composition capable of reducing uric acid and a preparation method thereof, comprising the following steps: sequentially crushing, drying, supercritical CO2, ultrasonic extraction and vacuum drying grape seeds to obtain grape seed extract; mixing chicory, mulberry leaves, plantain and purslane, and then sequentially performing freeze-drying, crushing and ultrasonic extraction to obtain a mixed extraction liquid, and vacuum drying to obtain a mixed plant extract; and mixing the grape seed extract, the mixed plant extract and soybean extract to obtain the plant source composition capable of reducing uric acid. The composition is composed of soybeans, grape seeds, chicory, mulberry leaves, plantain and purslane, all of which are homologous in medicine and food or widely used plants, have no specific toxicity targets of chemical drugs, and are suitable for long-term intervention of ordinary high uric acid people, drug contraindicated people and mild to moderate patients.

Description

Technical Field

[0001] This invention relates to the field of plant extract application technology, and more specifically, to a plant-derived composition that can lower uric acid, its preparation method, and its application. Background Technology

[0002] Hyperuricemia is a metabolic disease caused by excessive uric acid production and / or insufficient excretion, leading to a blood uric acid concentration exceeding the normal range. Long-term hyperuricemia can cause gouty arthritis, uric acid kidney stones, gouty nephropathy, and other diseases, severely impacting the normal lives of sufferers. Studies have found that foods high in animal protein, especially red meat, organ meats, and some seafood, are typically high in purines. Excessive intake of these high-purine foods increases uric acid production, thus raising blood uric acid levels. Insufficient water intake reduces uric acid excretion efficiency by 30%–40%. Alcoholic beverages, such as beer, not only contain purines themselves, but alcohol metabolism also inhibits the kidneys' metabolism of uric acid. Foods high in fructose accelerate ATP breakdown during metabolism, promoting uric acid production and increasing blood uric acid levels. With rising living standards, people have increasingly higher dietary demands, further increasing the incidence of hyperuricemia, which is showing a trend towards affecting younger people.

[0003] Current treatment primarily involves medication to lower uric acid. In the acute phase, colchicine and nonsteroidal anti-inflammatory drugs (NSAIDs) are used to relieve symptoms, while in the chronic phase, allopurinol, febuxostat (which inhibits uric acid production), or benzbromarone (which promotes uric acid excretion) are used. However, existing regimens have significant limitations: long-term use of chemical drugs can easily lead to liver and kidney toxicity, gastrointestinal reactions, or allergies (such as Stevens-Johnson syndrome caused by allopurinol). Benzbromarone is contraindicated in patients with kidney stones, and its efficacy is poor in some patients; patient compliance is reduced due to side effects and the need for long-term medication. Allopurinol may cause severe allergic reactions, such as the rare but fatal Stevens-Johnson syndrome (SJS) and toxic epidermal necrolysis, characterized by widespread skin erythema, blisters, and epidermal peeling, which may be accompanied by fever and mucosal damage. If these occur, the drug must be discontinued immediately and emergency treatment administered. Other possible side effects include allergic pneumonia and allergic vasculitis. In addition to allergic reactions, adverse reactions may include nausea, vomiting, diarrhea, abdominal pain, loss of appetite, abnormal liver function, aplastic anemia, and neurological reactions. Febuxostat has a lower incidence of severe allergic reactions compared to allopurinol, but it carries unique cardiovascular risks. Febuxostat may increase the risk of adverse cardiovascular events, including angina, myocardial infarction, stroke, and worsening heart failure, especially in patients with a history of cardiovascular disease or high-risk cardiovascular factors.

[0004] For patients with mild symptoms, lifestyle intervention is generally used. However, lifestyle intervention has little effect on lowering uric acid levels, only reducing blood uric acid by an average of about 10% to 20%. Strict dietary control can reduce quality of life and is difficult to maintain in the long term; excessive dieting can also lead to the breakdown of fat and the production of ketone bodies, which in turn inhibits uric acid excretion.

[0005] Plant extracts (such as soybean, chicory, and celery seed extracts) have attracted much attention due to their multi-target regulation of uric acid production and excretion, as well as their anti-inflammatory and antioxidant effects. Their natural components (such as peptides and polyphenols) can lower blood uric acid levels by inhibiting xanthine oxidase and regulating uric acid transporter protein expression, while simultaneously reducing inflammatory damage. Their safety profile is higher than that of chemical drugs, making them suitable for long-term adjuvant therapy. However, plant extracts face multiple challenges: the extraction process is complex, requiring enzymatic hydrolysate preparation and multiple separation and purification steps; the extraction process requires the use of large amounts of organic solvents, which may leave residues and harm the user's health. Therefore, there is an urgent need for a natural, safe, and highly effective plant extract for lowering uric acid. Summary of the Invention

[0006] In view of this, the present invention proposes a plant-derived composition that can lower uric acid, its preparation method and application, aiming to solve the problem that current drugs for lowering blood uric acid concentration have poor efficacy and strong side effects.

[0007] This invention provides a method for preparing a plant-derived composition that can lower uric acid, comprising the following steps: 1) Soybeans are subjected to cyclic freeze-thaw treatment and microwave extraction to obtain soybean extract A, a gaseous condensate, and microwave-treated soybeans; the microwave-treated soybeans are crushed and mixed with water for microwave extraction again; after microwave extraction, the mixture is filtered and the filtrate is freeze-dried to obtain soybean extract B; soybean extract A and soybean extract B are mixed to obtain soybean extract. 2) Grape seeds are crushed and dried to obtain grape seed powder. Supercritical CO2 is used as an extractant to remove the oil from the powder to obtain defatted grape seed powder. The defatted grape seed powder is mixed with ethanol and ultrasonically extracted to obtain grape seed extract. The extract is then vacuum dried to obtain grape seed extract. 3) Chicory, mulberry leaves, plantain, and purslane are mixed, freeze-dried, and crushed to obtain mixed plant powder. The mixed plant powder is mixed with ethanol and ultrasonically extracted to obtain a mixed extract. The mixed plant extract is obtained by vacuum drying. 4) The soybean extract prepared in step 1), the grape seed extract prepared in step 2), and the mixed plant extract prepared in step 3) are mixed to obtain a plant-derived composition that can lower uric acid.

[0008] Preferably, the particle size of the grape seed powder in step 2) is 100~200μm; The ultrasonic extraction power is 300~400W, the time is 10~20min, and the temperature is 0~15℃.

[0009] Preferably, the vacuum drying in step 2) has a pressure of 0.1~0.2MPa, a temperature of 0~20℃, and a time of 3~6h.

[0010] Preferably, the mixing ratio of chicory, mulberry leaves, plantain, and purslane in step 3) is 1~1.8:0.3~0.8:0.5~1.2:1~1.5; The freeze-drying temperature is -25 to -15°C, and the freeze-drying time is 24 to 72 hours.

[0011] Preferably, the ultrasonic extraction in step 3) uses a power of 300~400W, a time of 20~30min, and a temperature of 0~20℃. The vacuum drying process involves a pressure of 0.1~0.2 MPa, a temperature of 0~20℃, and a time of 3~6 hours.

[0012] Preferably, the mixing ratio of soybean extract, grape seed extract and mixed plant extract in step 4) is 1:0.2~0.5:2~3.

[0013] This invention provides a plant-derived composition that can lower uric acid, prepared by the above-described method.

[0014] The present invention also provides the application of the plant-derived composition that can lower uric acid in the preparation of uric acid-lowering drugs, comprising the following steps: Plant-derived compositions that can lower uric acid are combined with food science or pharmaceutically acceptable carriers to form food or clinically acceptable formulations. The formulations include liquid formulations, granules, tablets, powders, capsules, pellets, drops, injections, compressed candies, solid beverages, liquid beverages, gels, gummies, oral liquids, emulsions / lotions, and sprays.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: The composition consists of soybeans, grape seeds, chicory, mulberry leaves, plantain, and purslane, all of which are medicinal and edible plants or widely used plants. They do not have the "specific toxicity targets" of chemical drugs and are suitable for long-term intervention for ordinary people with high uric acid, people who are contraindicated for drugs, and patients with mild to moderate symptoms. During the extraction process, the protein and purine substances contained in soybeans are removed to the maximum extent, which can avoid allergic reactions caused by the protein in soybeans.

[0016] The preparation process first uses supercritical carbon dioxide to remove oil from soybeans and grape seeds, followed by low-temperature ultrasonic extraction and freeze-drying / vacuum drying. This avoids the chemical residues that may result from traditional solvent extraction, further improving safety for consumption. The entire preparation process is carried out under low-temperature conditions, effectively preserving the activity of the active substances in the plant source and preventing high-temperature degradation and destruction. The soybean oil and grape seed oil obtained through supercritical extraction can also be sold as value-added products, increasing revenue. Finally, the soybean meal after extraction contains no toxic substances and can be used to make animal feed, avoiding resource waste.

[0017] This product utilizes a synergistic chain of plant extracts—soybean extract inhibiting uric acid production, grape seed extract protecting the kidneys and promoting excretion, and a blend of plant extracts further promoting excretion and providing anti-inflammatory benefits—to avoid the limitations of single-component, limited-mechanism, and less effective treatments. The uric acid reduction is significantly better than lifestyle interventions, and it simultaneously alleviates joint inflammation caused by high uric acid, improving patients' quality of life. Furthermore, this plant-based composition is odorless, can be formulated into various dosage forms, and is easier to maintain long-term compared to dietary control. Detailed Implementation

[0018] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features, and embodiments of the present invention. It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the present invention.

[0019] Furthermore, regarding the numerical ranges in this invention, it should be understood that each intermediate value between the upper and lower limits of the range is also specifically disclosed. Every smaller range between any stated value or intermediate value within a stated range, and any other stated value or intermediate value within said range, is also included within this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0020] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0021] Various modifications and variations can be made to the specific embodiments described in this specification without departing from the scope or spirit of the invention, as will be apparent to those skilled in the art. Other embodiments derived from this specification will also be apparent to those skilled in the art. This specification and embodiments are merely exemplary.

[0022] The terms “include,” “including,” “have,” “contain,” etc., used in this article are all open-ended terms, meaning that they include but are not limited to.

[0023] This invention provides a method for preparing a plant-derived composition that can lower uric acid, comprising the following steps: 1) Soybeans are subjected to a cyclic freeze-thaw process and a first microwave extraction to obtain soybean extract A, a gaseous condensate, and microwave-treated soybeans; the microwave-treated soybeans are crushed and mixed with water for a second microwave extraction; after the microwave extraction is completed, the mixture is filtered and the filtrate is freeze-dried to obtain soybean extract B; soybean extract A and soybean extract B are mixed to obtain soybean extract. 2) Grape seeds are crushed and dried to obtain grape seed powder. Supercritical CO2 is used as an extractant to remove the oil from the powder to obtain defatted grape seed powder. The defatted grape seed powder is mixed with ethanol and ultrasonically extracted to obtain grape seed extract. The extract is then vacuum dried to obtain grape seed extract. 3) Chicory, mulberry leaves, plantain, and purslane are mixed, freeze-dried, and crushed to obtain mixed plant powder. The mixed plant powder is mixed with ethanol and ultrasonically extracted to obtain a mixed extract. The mixed plant extract is obtained by vacuum drying. 4) The soybean extract prepared in step 1), the grape seed extract prepared in step 2), and the mixed plant extract prepared in step 3) are mixed to obtain a plant-derived composition that can lower uric acid.

[0024] In this invention, the particle size of soybean meal in step 1) is 200~800μm, preferably 300~600μm, more preferably 400~500μm, and even more preferably 450μm.

[0025] Particle size determines the supercritical CO2 defatting efficiency and the mass transfer efficiency during subsequent alkali extraction. If the particle size is too fine, it is prone to clumping, which prevents supercritical CO2 from fully penetrating and results in incomplete defatting. If the particle size is too coarse, the specific surface area is small, making it difficult to extract the oil, and the alkali solution cannot quickly penetrate into the soybean meal, resulting in slow protein dissolution. The particle size range used in this application ensures both the contact efficiency between CO2 and oil and avoids clumping, significantly improving the extraction and alkali extraction effects.

[0026] In this invention, the alkaline extraction temperature in step 1) is 25~40℃, preferably 28~37℃, more preferably 30~35℃, and even more preferably 32℃; the alkaline extraction time is 24~48h, preferably 28~42h, more preferably 32~38h, and even more preferably 35h. Alkaline extraction is a process in which protein and active substances are slowly dissolved from soybean meal fiber. The temperature range of 25~40℃ is the stable range for active uric acid-lowering components such as polypeptides and flavonoids in soybeans, avoiding denaturation and inactivation of active components due to high temperature; the alkaline extraction time of 28~42h can achieve the stepwise dissolution of soybean protein and active components, which can remove crude protein in soybeans that is prone to causing allergies to the maximum extent, while retaining highly active uric acid-lowering polypeptides, and at the same time reducing the content of purine substances in soybeans, avoiding the increase of uric acid production due to purine intake.

[0027] In this invention, the cyclic freeze-thaw treatment in step 1) specifically involves freezing at -40°C for 1 hour, followed by thawing at 25°C for 0.5 hours, repeated 2-4 times. Through repeated alternation of low-temperature freezing and room-temperature thawing, the expansion and contraction forces generated by the freeze-thaw of water within soybean cells cause irreversible rupture of the soybean cell walls, disrupting the dense structure of the cells. This opens mass transfer channels for subsequent microwave extraction, making it easier for the active uric acid-lowering components within the cells to come into contact with the extraction solution, significantly improving extraction efficiency. Compared to soybeans that have not undergone freeze-thaw treatment, the dissolution rate of active ingredients can be increased by more than 40%.

[0028] In this invention, during the first microwave extraction in step 1), the microwave frequency is 2-3 GHz and the extraction time is 30-90 seconds. The microwave frequency of 2-3 GHz is the resonant frequency of the volatile active ingredients in soybeans, which can quickly cause the volatile uric acid-lowering components in soybeans to escape from the cells. The short extraction time of 30-90 seconds can avoid the decomposition of components caused by prolonged microwave action, thus ensuring the activity and integrity of the volatile components.

[0029] In this invention, gas condensation in step 1) is carried out at -5°C and -0.1 MPa. This low-temperature vacuum condition can quickly condense the escaped volatile active ingredients into a liquid state, avoiding component loss due to volatilization, and achieving stepwise extraction and complete preservation of the volatile and non-volatile uric acid-lowering components of soybeans.

[0030] In this invention, the pulverization in step 1) specifically involves pulverizing to a particle size of 0.5 mm; the mass ratio of soybeans to water is 1:4.

[0031] In this invention, the second microwave extraction in step 1) is carried out under a nitrogen atmosphere with a nitrogen flow rate of 5 L / min; the microwave frequency is 1-2 GHz, the magnetic field strength is 150-200 mT, and the second microwave extraction lasts for 30-90 seconds. The nitrogen atmosphere isolates oxygen, preventing the polyphenols, polypeptides, and other active ingredients in soybeans from being oxidized and deactivated during microwave extraction, thus ensuring the uric acid-lowering activity of the extract. The microwave frequency of 1-2 GHz combined with the magnetic field strength of 150-200 mT can further destroy the residual structure of soybean cells through the synergistic effect of microwave thermal and non-thermal effects, allowing non-volatile active ingredients to fully dissolve. The extraction time of 30-90 seconds ensures the dissolution rate while preventing degradation of components due to prolonged microwave exposure.

[0032] In this invention, the freeze-drying temperature in step 1) is -20°C and the pressure is -0.1 MPa. In this invention, the particle size of the grape seed powder in step 2) is 100~200 μm, preferably 120~180 μm, more preferably 140~160 μm, and even more preferably 150 μm.

[0033] The polypeptide components in soybean extract can specifically inhibit the activity of xanthine oxidase, reducing uric acid production at its source. At the same time, flavonoid components can regulate the expression of renal uric acid transporter proteins and promote uric acid excretion, achieving a dual effect of "inhibition of production + promotion of excretion". Moreover, the soybean extract prepared by this process removes most of the crude protein and purines, avoiding the risk of protein allergy from soybeans and the problem of elevated uric acid caused by purine intake.

[0034] The active ingredients in grape seeds are mainly found inside the cells. During the subsequent ultrasonic extraction process, the cell walls can be broken by ultrasound. Therefore, the particle size of grape seed powder needs to meet two conditions: the ultrasonic energy can be transmitted evenly and the powder is not easy to clump during ultrasound. Setting the particle size to 160~180μm can achieve a cell wall breakage rate of ≥85%.

[0035] In this invention, the ultrasonic extraction power in step 2) is 300-400W, preferably 320-380W, more preferably 340-360W, and even more preferably 350W; the time is 10-20 min, preferably 12-18 min, more preferably 14-16 min, and even more preferably 15 min; the temperature is 0-15℃, preferably 5-12℃, more preferably 8-10℃, and even more preferably 9℃. The ultrasonic power of 300-400W can generate a sufficiently strong cavitation effect, destroying the cell walls and cell membranes of grape seeds, allowing the active ingredients to be released into the ethanol extract; the extraction time of 10-20 min can achieve sufficient dissolution of the active ingredients, avoiding low dissolution rates due to too short an extraction time, or oxidation of the ingredients due to too long an extraction time; the low-temperature extraction environment of 0-15℃ can effectively inhibit the oxidative decomposition of active ingredients such as grape seed polyphenols and proanthocyanidins, while avoiding the damage to the components caused by the local high temperature generated by the ultrasonic cavitation effect, ensuring the antioxidant and renal protective activities of the extract.

[0036] In this invention, the vacuum drying pressure in step 2) is 0.1~0.2 MPa, preferably 0.12~0.18 MPa, more preferably 0.14~0.16 MPa, and even more preferably 0.15 MPa; the temperature is 0~20℃, preferably 5~15℃, more preferably 8~12℃, and even more preferably 10℃; the time is 3~6 h, preferably 3.5~5.5 h, more preferably 4~5 h, and even more preferably 4.5 h. The vacuum of 0.1~0.2 MPa combined with the low temperature of 0~20℃ can quickly remove ethanol from the grape seed extract while avoiding denaturation of heat-sensitive components such as proanthocyanidins caused by heat drying, thus ensuring the activity of the extract. The drying time of 3~6 h allows for thorough drying of the extract, resulting in a powder moisture content ≤5%, facilitating subsequent storage and mixing. Furthermore, the dried extract powder has good flowability and is free of clumping.

[0037] The proanthocyanidins and polyphenols in grape seeds have strong antioxidant properties, which can scavenge oxygen free radicals in the kidneys, reduce oxidative stress damage to the kidneys caused by high uric acid, and protect the normal function of renal tubular epithelial cells. At the same time, they can regulate renal microcirculation, enhance the kidneys' ability to excrete uric acid, and their antioxidant effect can reduce joint inflammation caused by high uric acid, achieving a triple effect of "kidney protection + excretion promotion + anti-inflammation". When combined with soybean extract, they form a synergistic effect to enhance the overall uric acid-lowering effect.

[0038] In this invention, the mixing ratio of chicory, mulberry leaves, plantain, and purslane in step 3) is 1~1.8:0.3~0.8:0.5~1.2:1~1.5, preferably 1.2~1.6:0.4~0.7:0.7~1.0:1.1~1.4, more preferably 1.4~1.5:0.5~0.6:0.8~0.9:1.2~1.3, and even more preferably 1.45:0.55:0.85:1.25.

[0039] This ratio represents the optimal synergistic effect of the four plants: chicoric acid in chicory can inhibit xanthine oxidase and promote uric acid excretion; DNJ in mulberry leaves can reduce the absorption of purines in the intestines; plantain glycosides in plantain can increase kidney urine output and promote the excretion of uric acid in urine; and flavonoids in purslane can alleviate joint inflammation and kidney damage. When these four components are combined in this ratio, a full-chain synergistic effect of "enzyme inhibition + absorption reduction + excretion promotion + anti-inflammation" can be achieved. Compared with single plant extracts, the uric acid-lowering effect can be increased by more than 60%, and the anti-inflammatory effect is significantly enhanced.

[0040] In this invention, the freeze-drying temperature in step 3) is -25~-15℃, preferably -23~-17℃, more preferably -21~-19℃, and even more preferably -20℃; the freeze-drying time is 24~72h, preferably 36~60h, more preferably 42~54h, and even more preferably 45~50h.

[0041] In this invention, the ultrasonic extraction power in step 3) is 300~400W, preferably 320~380W, more preferably 340~360W, and even more preferably 350W; the time is 20~30min, preferably 22~28min, more preferably 24~26min, and even more preferably 25min; the temperature is 0~20℃, preferably 5~18℃, more preferably 10~15℃, and even more preferably 12℃.

[0042] In this invention, the vacuum drying pressure in step 3) is 0.1~0.2 MPa, preferably 0.12~0.18 MPa, more preferably 0.14~0.16 MPa, and even more preferably 0.15 MPa; the temperature is 0~20℃, preferably 5~15℃, more preferably 8~12℃, and even more preferably 10℃; the time is 3~6 h, preferably 3.5~5.5 h, more preferably 4~5 h, and even more preferably 4.5 h. Consistent with the vacuum drying principle of grape seed extract, this low-temperature vacuum condition can quickly remove ethanol from the extract, maximizing the retention of the activity of various heat-sensitive active ingredients in the mixed plants. The dried extract powder has a moisture content of ≤5%, good flowability, and good solubility, facilitating subsequent mixing with soybean extract and grape seed extract.

[0043] In this invention, the mixing ratio of soybean extract, grape seed extract and mixed plant extract in step 4) is 1:0.2~0.5:2~3, 1:0.3~0.4:2.2~2.8, more preferably 1:0.35~0.38:2.4~2.6, and even more preferably 1:0.37:2.5.

[0044] This invention provides a plant-derived composition that can lower uric acid, prepared by the above-described method.

[0045] The present invention also provides the application of the plant-derived composition that can lower uric acid in the preparation of uric acid-lowering drugs, comprising the following steps: Plant-derived compositions that can lower uric acid are combined with food science or pharmaceutically acceptable carriers to form food or clinically acceptable formulations. Pharmaceutical preparations include liquid preparations, granules, tablets, powders, capsules, pellets, drops, injections, compressed candies, solid beverages, liquid beverages, gels, gummies, oral liquids, emulsions / lotions, and sprays.

[0046] Example 1 (1) Preparation of soybean extract Soybeans were frozen at -40℃ for 1 hour, then thawed at 25℃ for 0.5 hours, and this cycle was repeated twice to complete the freeze-thaw treatment. The soybeans were then placed in a microwave reactor, and microwave extraction was performed at a frequency of 2 GHz for 30 seconds per extraction. After microwave extraction, the gas in the microwave reactor was condensed at -5℃ and -0.1 MPa to obtain soybean extract A. The microwave-extracted soybeans were then pulverized to a particle size of 0.5 mm. The soybeans and deionized water were then mixed at a mass ratio of 1:4 and heated at 40℃ for 500 rpm. Soybean slurry was obtained by wetting the soybeans for 10 minutes. The soybean slurry was then placed in a microwave reactor, and nitrogen gas was introduced into the reactor at a flow rate of 5 L / min. The microwave frequency was then controlled at 1 GHz and the magnetic field strength at 150 mT for a second microwave extraction of 30 s. The soybean slurry after the second microwave extraction was filtered through a 0.1 μm ceramic membrane, and the filtrate was freeze-dried at -20℃ and -0.1 MPa to remove water, yielding soybean extract B. Soybean extract A and soybean extract B were then mixed to obtain soybean extract.

[0047] (2) Preparation of grape seed extract Grape seeds were washed, crushed, and dried at 55℃ for 3 hours. The resulting powder was sieved to obtain grape seed powder with a particle size of 150 μm. Supercritical CO2 extraction (extraction pressure 28 MPa, extraction temperature 32℃, CO2 flow rate 18 L / h) was used to remove oil from the powder, yielding defatted grape seed powder. The defatted grape seed powder was mixed with 70% ethanol solution at a material-to-liquid ratio of 1:20 (g / mL) and placed in an ultrasonic extractor. The mixture was ultrasonically extracted for 15 minutes at 350 W and 9℃. After extraction, the mixture was centrifuged at 4000 r / min for 10 minutes, and the supernatant (grape seed extract) was collected. The grape seed extract was placed in a vacuum drying oven and dried for 4.5 hours at 0.15 MPa and 10℃. The resulting powder was then crushed to obtain the grape seed extract.

[0048] (3) Preparation of mixed plant extracts Chicory, mulberry leaves, plantain, and purslane were weighed in a mass ratio of 1.45:0.55:0.85:1.25, mixed, and placed in a freeze dryer. The mixture was freeze-dried at -20℃ and 10Pa vacuum for 48 hours. After pulverization, the powder was passed through an 80-mesh sieve to obtain a mixed plant powder. The mixed plant powder was then mixed with a 60% ethanol solution at a material-to-liquid ratio of 1:18 (g / mL) and placed in an ultrasonic extractor. Ultrasonic extraction was performed at 350W and 12℃ for 25 minutes. After extraction, the mixture was centrifuged at 4000 rpm for 10 minutes, and the supernatant (mixed extract) was collected. The mixed extract was then placed in a vacuum drying oven and dried at 0.15MPa and 10℃ for 4.5 hours. After pulverization, the mixed plant extract was obtained.

[0049] (4) Preparation of the composition Weigh the soybean extract prepared in step 1, the grape seed extract prepared in step 2, and the mixed plant extract prepared in step 3 according to a mass ratio of 1:0.37:2.5, place them in a mixer, and mix for 30 minutes at a speed of 300 r / min to obtain a plant-derived composition that can lower uric acid.

[0050] Example 2 (1) Preparation of soybean extract Soybeans were frozen at -40℃ for 1 hour, then thawed at 25℃ for 0.5 hours, and this cycle was repeated twice to complete the freeze-thaw treatment. The soybeans were then placed in a microwave reactor, and microwave extraction was performed at 2 GHz for 30 seconds per cycle. After microwave extraction, the gas in the microwave reactor was condensed at -5℃ and -0.1 MPa to obtain soybean extract A. The microwave-extracted soybeans were then pulverized to a particle size of 0.5 mm. The soybeans and deionized water were then mixed at a mass ratio of 1:4 and heated at 40℃ and 500 rpm. Soybean slurry was obtained by wetting the soybeans for 10 minutes. The soybean slurry was then placed in a microwave reactor, and nitrogen gas was introduced into the reactor at a flow rate of 5 L / min. The microwave frequency was then controlled at 1 GHz and the magnetic field strength at 150 mT for a second microwave extraction of 30 s. The soybean slurry after the second microwave extraction was filtered through a 0.1 μm ceramic membrane, and the filtrate was freeze-dried at -20℃ and -0.1 MPa to remove water, yielding soybean extract B. Soybean extract A and soybean extract B were then mixed to obtain the soybean extract.

[0051] (2) Preparation of grape seed extract Grape seeds were washed, crushed, and dried at 58℃ for 2.5 h. The resulting powder was sieved to obtain grape seed powder with a particle size of 140 μm. Supercritical CO2 extraction was used to remove oil from the powder (extraction pressure 27 MPa, extraction temperature 31℃, CO2 flow rate 17 L / h) to obtain defatted grape seed powder. The defatted grape seed powder was mixed with 68% ethanol solution at a material-to-liquid ratio of 1:19 (g / mL) and placed in an ultrasonic extractor. The mixture was ultrasonically extracted for 14 min at a power of 340 W and a temperature of 8℃. After extraction, the mixture was centrifuged at 3800 r / min for 11 min, and the supernatant was collected. The supernatant was placed in a vacuum drying oven and dried for 4 h at a pressure of 0.14 MPa and a temperature of 9℃. The resulting extract was then crushed to obtain grape seed extract.

[0052] (3) Preparation of mixed plant extracts Chicory, mulberry leaves, plantain, and purslane were weighed in a mass ratio of 1.4:0.5:0.8:1.2, mixed, and placed in a freeze dryer. The mixture was freeze-dried at -21℃ and 12Pa vacuum for 45 hours. After pulverization, the powder was passed through an 80-mesh sieve to obtain a mixed plant powder. The mixed plant powder was then mixed with a 58% ethanol solution at a material-to-liquid ratio of 1:17 (g / mL) and placed in an ultrasonic extractor. Ultrasonic extraction was performed at 340W and 10℃ for 24 minutes. After extraction, the mixture was centrifuged at 3800 r / min for 11 minutes, and the supernatant was collected. The supernatant was placed in a vacuum drying oven and dried at 0.14 MPa and 9℃ for 4 hours. After pulverization, the mixed plant extract was obtained.

[0053] (4) Preparation of the composition Weigh the soybean extract prepared in step 1, the grape seed extract prepared in step 2, and the mixed plant extract prepared in step 3 according to a mass ratio of 1:0.35:2.4, place them in a mixer, and mix for 35 minutes at a speed of 280 r / min to obtain a plant-derived composition that can lower uric acid.

[0054] Example 3 (1) Preparation of soybean extract Soybeans were frozen at -40℃ for 1 hour, then thawed at 25℃ for 0.5 hours, and this cycle was repeated twice to complete the freeze-thaw treatment. The soybeans were then placed in a microwave reactor, and microwave extraction was performed at 2 GHz for 30 seconds per cycle. After microwave extraction, the gas in the microwave reactor was condensed at -5℃ and -0.1 MPa to obtain soybean extract A. The microwave-extracted soybeans were then pulverized to a particle size of 0.5 mm. The soybeans and deionized water were then mixed at a mass ratio of 1:4 and heated at 40℃ and 500 rpm. Soybean slurry was obtained by wetting the soybeans for 10 minutes. The soybean slurry was then placed in a microwave reactor, and nitrogen gas was introduced into the reactor at a flow rate of 5 L / min. The microwave frequency was then controlled at 1 GHz and the magnetic field strength at 150 mT for a second microwave extraction of 30 s. The soybean slurry after the second microwave extraction was filtered through a 0.1 μm ceramic membrane, and the filtrate was freeze-dried at -20℃ and -0.1 MPa to remove water, yielding soybean extract B. Soybean extract A and soybean extract B were then mixed to obtain the soybean extract.

[0055] (2) Preparation of grape seed extract Grape seeds were washed, crushed, and dried at 52℃ for 3.2 h. The resulting powder was sieved to obtain grape seed powder with a particle size of 160 μm. Supercritical CO2 extraction was used to remove oil from the powder (extraction pressure 29 MPa, extraction temperature 33℃, CO2 flow rate 19 L / h) to obtain defatted grape seed powder. The defatted grape seed powder was mixed with 72% ethanol solution at a material-to-liquid ratio of 1:21 (g / mL) and placed in an ultrasonic extractor. The mixture was ultrasonically extracted for 16 min at a power of 360 W and a temperature of 10℃. After extraction, the mixture was centrifuged at 4200 r / min for 9 min, and the supernatant was collected. The supernatant was placed in a vacuum drying oven and dried for 5 h at a pressure of 0.16 MPa and a temperature of 11℃. The resulting extract was then crushed to obtain grape seed extract.

[0056] (3) Preparation of mixed plant extracts Chicory, mulberry leaves, plantain, and purslane were weighed in a mass ratio of 1.5:0.6:0.9:1.3, mixed, and placed in a freeze dryer. The mixture was freeze-dried at -19℃ and 8 Pa for 50 hours. After pulverization, the powder was passed through an 80-mesh sieve to obtain a mixed plant powder. The mixed plant powder was then mixed with a 62% ethanol solution in a material-to-liquid ratio of 1:19 (g / mL). The mixture was placed in an ultrasonic extractor and ultrasonically extracted for 26 minutes at 360W and 15℃. After extraction, the mixture was centrifuged at 4200 r / min for 9 minutes, and the supernatant was collected. The supernatant was placed in a vacuum drying oven and dried for 5 hours at 0.16 MPa and 11℃. After pulverization, the mixed plant extract was obtained.

[0057] (4) Preparation of the composition Weigh the soybean extract prepared in step 1, the grape seed extract prepared in step 2, and the mixed plant extract prepared in step 3 according to a mass ratio of 1:0.38:2.6, place them in a mixer, and mix for 28 minutes at a speed of 320 r / min to obtain a plant-derived composition that can lower uric acid.

[0058] Example 4 (1) Preparation of soybean extract Soybeans were frozen at -40℃ for 1 hour, then thawed at 25℃ for 0.5 hours, and this cycle was repeated twice to complete the freeze-thaw treatment. The soybeans were then placed in a microwave reactor, and microwave extraction was performed at 2 GHz for 30 seconds per cycle. After microwave extraction, the gas in the microwave reactor was condensed at -5℃ and -0.1 MPa to obtain soybean extract A. The microwave-extracted soybeans were then pulverized to a particle size of 0.5 mm. The soybeans and deionized water were then mixed at a mass ratio of 1:4 and heated at 40℃ and 500 rpm. Soybean slurry was obtained by wetting the soybeans for 10 minutes. The soybean slurry was then placed in a microwave reactor, and nitrogen gas was introduced into the reactor at a flow rate of 5 L / min. The microwave frequency was then controlled at 1 GHz and the magnetic field strength at 150 mT for a second microwave extraction of 30 s. The soybean slurry after the second microwave extraction was filtered through a 0.1 μm ceramic membrane, and the filtrate was freeze-dried at -20℃ and -0.1 MPa to remove water, yielding soybean extract B. Soybean extract A and soybean extract B were then mixed to obtain the soybean extract.

[0059] (2) Preparation of grape seed extract Grape seeds were washed, crushed, and dried at 60℃ for 2 hours. The resulting powder was sieved to obtain grape seed powder with a particle size of 120 μm. Supercritical CO2 extraction was used to remove oil from the powder (extraction pressure 26 MPa, extraction temperature 30℃, CO2 flow rate 16 L / h) to obtain defatted grape seed powder. The defatted grape seed powder was mixed with 65% ethanol solution at a material-to-liquid ratio of 1:18 (g / mL) and placed in an ultrasonic extractor. The mixture was ultrasonically extracted for 12 minutes at a power of 320 W and a temperature of 5℃. After extraction, the mixture was centrifuged at 3500 r / min for 12 minutes, and the supernatant was collected. The supernatant was placed in a vacuum drying oven and dried for 3.5 hours at a pressure of 0.12 MPa and a temperature of 8℃. The resulting extract was then crushed to obtain grape seed extract.

[0060] (3) Preparation of mixed plant extracts Chicory, mulberry leaves, plantain, and purslane were weighed in a mass ratio of 1.2:0.4:0.7:1.1, mixed, and placed in a freeze dryer. The mixture was freeze-dried at -23℃ and 15Pa vacuum for 36 hours. After pulverization, the powder was passed through an 80-mesh sieve to obtain a mixed plant powder. The mixed plant powder was then mixed with a 55% ethanol solution at a material-to-liquid ratio of 1:16 (g / mL) and placed in an ultrasonic extractor. The mixture was ultrasonically extracted for 22 minutes at 320W and 5℃. After extraction, the mixture was centrifuged at 3500 rpm for 12 minutes, and the supernatant was collected. The supernatant was placed in a vacuum drying oven and dried for 3.5 hours at 0.12 MPa and 8℃. After pulverization, the mixed plant extract was obtained.

[0061] (4) Preparation of the composition Weigh the soybean extract prepared in step 1, the grape seed extract prepared in step 2, and the mixed plant extract prepared in step 3 according to a mass ratio of 1:0.3:2.2, place them in a mixer, and mix for 40 minutes at a speed of 250 r / min to obtain a plant-derived composition that can lower uric acid.

[0062] Example 5 (1) Preparation of soybean extract Soybeans were frozen at -40℃ for 1 hour, then thawed at 25℃ for 0.5 hours, and this cycle was repeated twice to complete the freeze-thaw treatment. The soybeans were then placed in a microwave reactor, and microwave extraction was performed at 2 GHz for 30 seconds per cycle. After microwave extraction, the gas in the microwave reactor was condensed at -5℃ and -0.1 MPa to obtain soybean extract A. The microwave-extracted soybeans were then pulverized to a particle size of 0.5 mm. The soybeans and deionized water were then mixed at a mass ratio of 1:4 and heated at 40℃ and 500 rpm. Soybean slurry was obtained by wetting the soybeans for 10 minutes. The soybean slurry was then placed in a microwave reactor, and nitrogen gas was introduced into the reactor at a flow rate of 5 L / min. The microwave frequency was then controlled at 1 GHz and the magnetic field strength at 150 mT for a second microwave extraction of 30 s. The soybean slurry after the second microwave extraction was filtered through a 0.1 μm ceramic membrane, and the filtrate was freeze-dried at -20℃ and -0.1 MPa to remove water, yielding soybean extract B. Soybean extract A and soybean extract B were then mixed to obtain the soybean extract.

[0063] (2) Preparation of grape seed extract Grape seeds were washed, crushed, and dried at 50℃ for 3.5 h. The resulting powder was sieved to obtain grape seed powder with a particle size of 180 μm. Supercritical CO2 extraction was used to remove oil from the powder (extraction pressure 30 MPa, extraction temperature 34℃, CO2 flow rate 20 L / h) to obtain defatted grape seed powder. The defatted grape seed powder was mixed with 75% ethanol solution at a material-to-liquid ratio of 1:22 (g / mL) and placed in an ultrasonic extractor. The mixture was ultrasonically extracted for 18 min at a power of 380 W and a temperature of 12℃. After extraction, the mixture was centrifuged at 4500 r / min for 8 min, and the supernatant was collected. The supernatant was placed in a vacuum drying oven and dried for 5.5 h at a pressure of 0.18 MPa and a temperature of 12℃. The resulting extract was then crushed to obtain grape seed extract.

[0064] (3) Preparation of mixed plant extracts Chicory, mulberry leaves, plantain, and purslane were weighed in a mass ratio of 1.6:0.7:1.0:1.4, mixed, and placed in a freeze dryer. The mixture was freeze-dried at -17℃ and 5Pa vacuum for 60 hours. After pulverization, the powder was passed through an 80-mesh sieve to obtain a mixed plant powder. The mixed plant powder was then mixed with a 65% ethanol solution at a material-to-liquid ratio of 1:20 (g / mL) and placed in an ultrasonic extractor. Ultrasonic extraction was performed at 380W and 18℃ for 28 minutes. After extraction, the mixture was centrifuged at 4500 rpm for 8 minutes, and the supernatant was collected. The supernatant was placed in a vacuum drying oven and dried at 0.18MPa and 12℃ for 5.5 hours. After pulverization, the mixed plant extract was obtained.

[0065] (4) Preparation of the composition Weigh the soybean extract prepared in step 1, the grape seed extract prepared in step 2, and the mixed plant extract prepared in step 3 according to a mass ratio of 1:0.4:2.8, place them in a mixer, and mix for 25 minutes at a speed of 350 r / min to obtain a plant-derived composition that can lower uric acid.

[0066] The effects of the plant-derived compositions prepared in Examples 1-5 on lowering uric acid were tested, and the methods and data are as follows: 1. Selection of the subject population Diagnostic criteria: Meets the diagnostic criteria of the "Chinese Guidelines for the Diagnosis and Treatment of Hyperuricemia and Gout (2021)", namely, fasting blood uric acid levels on two separate days: male > 420 μmol / L, female > 360 μmol / L (women need to exclude pregnancy and lactation). Inclusion criteria: ① Age 18-65 years; ② No use of uric acid-lowering drugs (such as allopurinol, febuxostat) or drugs affecting uric acid metabolism (such as diuretics) in the past month; ③ No severe liver or kidney dysfunction (ALT, AST < 2 times the upper limit of normal, serum creatinine < 133 μmol / L); ④ No acute gout attack; ⑤ Willing to sign informed consent form; Exclusion criteria: ① Individuals allergic to the test ingredients such as soybeans, chicory, mulberry leaves, plantain, and purslane; ② Individuals with a history of kidney stones, chronic kidney disease (CKD stage 3 or above), or cardiovascular disease; ③ Pregnant or breastfeeding women; ④ Individuals who have participated in other clinical trials within the past 3 months. Sample size: A total of 120 cases were included and divided into 4 groups of 30 cases each, in a ratio of 1:1:1:1. There were no dropouts.

[0067] 2. Experimental Design Table 1

[0068] Control variables: All subjects maintained a uniform low-purine diet (daily purine intake <300mg) during the experiment, avoided alcohol and high-fructose beverages, drank ≥2000mL of water daily, avoided strenuous exercise, and maintained a regular schedule.

[0069] II. Detection Indicators and Methods 1. Effectiveness indicators Venous blood samples were collected at week 0 (baseline), week 2, week 4, and week 6 of the experiment to measure fasting serum uric acid (UA) concentration.

[0070] 2. Safety Indicators The levels of ALT (alanine aminotransferase), AST (aspartate aminotransferase), and BUN (blood urea nitrogen) were measured at weeks 0 and 6 of the experiment. White blood cell (WBC), red blood cell (RBC), and platelet (PLT) levels were measured at weeks 0 and 6 of the experiment. Record the gastrointestinal reactions (nausea, diarrhea), skin reactions (rash), dizziness, and other symptoms that occur during the medication period, and classify them as "mild (does not affect daily life), moderate (affects daily life), and severe (requires discontinuation of medication)".

[0071] III. Test Results Table 2. Changes in serum uric acid (UA) levels in each group (unit: μmol / L, mean ± standard deviation)

[0072] In all embodiments, the serum uric acid levels of each group showed differentiated trends. In the placebo group, serum uric acid did not decrease significantly within 6 weeks, but fluctuated slightly, with the decrease being less than 3%. In the low-dose group (1g composition daily), serum uric acid decreased by 13.0% to 13.5% within 6 weeks, showing a certain uric acid-lowering effect. The medium-dose group (2g composition daily) showed a significant uric acid-lowering effect, with an average decrease of 20.5% to 21.0% in serum uric acid within 6 weeks. The decrease was basically consistent in all embodiments, which was close to the uric acid-lowering effect of the positive control group, allopurinol tablets (average decrease of 23.5% to 24.0% within 6 weeks). Moreover, the serum uric acid level in the medium-dose group showed a significant decrease from the second week of the experiment, and the decrease gradually increased with the extension of the administration time, showing a stable trend of efficacy.

[0073] Table 3. Changes in serum creatinine (CREA) and xanthine oxidase (XOD) in each group (difference from baseline at 6 weeks)

[0074] Creatinine levels: In the placebo group, there was no significant change in creatinine levels from baseline after 6 weeks. In the low-dose group, creatinine levels decreased slightly (difference of -4.3 to -4.9 μmol / L). In the medium-dose group, the decrease in creatinine levels was the most significant, with a difference of -7.0 to -7.6 μmol / L, which was significantly better than that in the positive control group (difference of -3.2 to -3.7 μmol / L). This indicates that the plant-derived compositions prepared in each example can effectively improve renal function at medium doses, and their renal protective effect is superior to that of allopurinol.

[0075] XOD index: There was no significant change in XOD activity in the placebo group, a slight decrease in XOD activity in the low-dose group (difference of -1.0 to -1.2 U / L), and a significant decrease in XOD activity in the medium-dose group (difference of -1.9 to -2.2 U / L). The inhibitory effect was basically the same as that in the positive control group (difference of -2.1 to -2.4 U / L), indicating that the plant-derived composition can effectively inhibit xanthine oxidase activity, reduce uric acid production from the source, and the inhibitory effect at the medium dose is comparable to that of allopurinol, a commonly used uric acid-lowering drug in clinical practice.

[0076] 3. Safety Results (1) Liver and kidney function and routine blood tests At week 6 of the experiment, all groups had ALT, AST, BUN, and routine blood tests within the normal range, and no cases showed abnormal liver or kidney function; among them: Placebo group: No change in ALT (baseline 25.3±5.2 U / L, 6 weeks 24.8±4.9 U / L); Medium-dose group: ALT decreased slightly (baseline 26.1±5.5U / L, 6 weeks 23.7±4.8U / L, P>0.05); Positive control group: 2 cases showed a slight increase in ALT (from 24.5 U / L to 32.1 U / L, which did not exceed the upper limit of normal).

[0077] (2) Incidence of adverse reactions Table 4. Results of Adverse Reactions

[0078] In each embodiment, the incidence of adverse reactions in both the placebo group and the low-dose group was 3.3%, with only mild gastrointestinal reactions (nausea / diarrhea) occurring, and no other adverse reactions such as skin reactions or dizziness. No serious adverse reactions occurred. The overall incidence of adverse reactions in the medium-dose group was 3.3% to 6.7%, all of which were mild gastrointestinal reactions. There were no skin reactions, dizziness, or other symptoms. None of the adverse reactions affected the normal life of the subjects, and there was no need to discontinue the medication. The overall incidence of adverse reactions in the positive control group was 16.7% to 23.3%. In addition to gastrointestinal reactions, skin rashes, dizziness and other symptoms also occurred. The types of adverse reactions were more diverse and the incidence was significantly higher than that in each dosage group of the plant-derived composition. Overall, the incidence of adverse reactions of the plant-derived compositions prepared in each embodiment was significantly lower than that of allopurinol tablets at all dosages, and there were no serious adverse reactions, demonstrating a clear safety advantage.

[0079] 4. Frequency of gout attacks During the experiments in all examples, the incidence of acute gout attacks was 10.0% (3 / 30) in the placebo group, 3.3% (1 / 30) in the low-dose group, 0% in the medium-dose group, and 3.3% (1 / 30) in the positive control group. The gout prevention effects of each dose group in each example were significantly better than the placebo group (P < 0.05) and comparable to the allopurinol tablets in the positive control group, indicating that the plant-derived composition can effectively reduce the risk of gout attacks in individuals with hyperuricemia at medium doses.

[0080] IV. Experimental Conclusions Efficacy: The plant-derived composition prepared in the examples (2g daily) can significantly reduce serum uric acid levels in people with hyperuricemia (a decrease of 20.7% in 6 weeks), with effects comparable to allopurinol. It can also inhibit xanthine oxidase activity (reducing uric acid production), improve renal function (reducing creatinine), and reduce the risk of gout attacks. Safety: The composition has a low incidence of adverse reactions (6.7%), no hepatotoxicity or nephrotoxicity, and its safety is significantly better than that of the chemical drug allopurinol (adverse reaction incidence 20.0%). Applicability: It meets the advantages of the original invention of "medicine and food from the same source, without specific toxic targets", and is suitable for long-term intervention in ordinary people with high uric acid, people who are contraindicated for drugs and patients with mild to moderate symptoms. It is also convenient to take (capsule dosage form) and has high patient compliance.

[0081] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the specific implementation of the present invention. Any modifications or equivalent substitutions that do not depart from the spirit and scope of the present invention should be covered within the scope of protection of the claims of the present invention.

Claims

1. A method for preparing a plant-derived composition that can lower uric acid, characterized in that, Includes the following steps: 1) Soybeans are subjected to cyclic freeze-thaw treatment and microwave extraction to obtain soybean extract A, a gaseous condensate, and microwave-treated soybeans; the microwave-treated soybeans are crushed and mixed with water for microwave extraction again; after microwave extraction, the mixture is filtered and the filtrate is freeze-dried to obtain soybean extract B; soybean extract A and soybean extract B are mixed to obtain soybean extract. 2) Grape seeds are crushed and dried to obtain grape seed powder. Supercritical CO2 is used as an extractant to remove the oil from the powder to obtain defatted grape seed powder. The defatted grape seed powder is mixed with ethanol and ultrasonically extracted to obtain grape seed extract. The extract is then vacuum dried to obtain grape seed extract. 3) Chicory, mulberry leaves, plantain, and purslane are mixed, freeze-dried, and crushed to obtain mixed plant powder. The mixed plant powder is mixed with ethanol and ultrasonically extracted to obtain a mixed extract. The mixed plant extract is obtained by vacuum drying. 4) The soybean extract prepared in step 1), the grape seed extract prepared in step 2), and the mixed plant extract prepared in step 3) are mixed to obtain a plant-derived composition that can lower uric acid.

2. The method for preparing a plant-derived composition that can lower uric acid according to claim 1, characterized in that, The particle size of the grape seed powder mentioned in step 2) is 100~200μm; The ultrasonic extraction power is 300~400W, the time is 10~20min, and the temperature is 0~15℃.

3. A method for preparing a plant-derived composition that can lower uric acid according to claim 1 or 2, characterized in that, The vacuum drying process described in step 2) involves a pressure of 0.1~0.2MPa, a temperature of 0~20℃, and a time of 3~6h.

4. The method for preparing a plant-derived composition that can lower uric acid according to claim 3, characterized in that, The mixing ratio of chicory, mulberry leaves, plantain, and purslane in step 3) is 1~1.8:0.3~0.8:0.5~1.2:1~1.5; The freeze-drying temperature is -25 to -15°C, and the freeze-drying time is 24 to 72 hours.

5. A method for preparing a plant-derived composition that can lower uric acid according to claim 4, characterized in that, The ultrasonic extraction described in step 3) uses a power of 300~400W, a time of 20~30min, and a temperature of 0~20℃. The vacuum drying process involves a pressure of 0.1~0.2 MPa, a temperature of 0~20℃, and a time of 3~6 hours.

6. A method for preparing a plant-derived composition that can lower uric acid according to claim 5, characterized in that, The mixing ratio of soybean extract, grape seed extract, and mixed plant extracts mentioned in step 4) is 1:0.2~0.5:2~3.

7. A plant-derived composition for lowering uric acid prepared by the method of any one of claims 1 to 6.

8. The use of the plant-derived composition for lowering uric acid according to claim 7 in the preparation of uric acid-lowering drugs, characterized in that, Includes the following steps: Plant-derived compositions that can lower uric acid are combined with food science or pharmaceutically acceptable carriers to form food or clinically acceptable formulations. The formulations include liquid formulations, granules, tablets, powders, capsules, pellets, drops, injections, compressed candies, solid beverages, liquid beverages, gels, gummies, oral liquids, emulsions / lotions, and sprays.

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