Microorganism rotating field sterilization device and flow rate measurement method

Abstract

The application relates to the technical field of microorganism sterilization, and discloses a microorganism rotating field sterilization device and a flow velocity determination method, which comprise a magnetic stirrer and a camera, the upper surface of the magnetic stirrer is provided with a beaker, the inside of the beaker is provided with a sterile rotor, the outer wall of the beaker is respectively provided with an arc-shaped frame and a fixing frame, the inside of the fixing frame is rotationally connected with a threaded rod, the outer wall of the threaded rod is threadedly connected in the inside of the arc-shaped frame, the upper surface of the fixing frame is fixedly connected with a fixing block, the inside of the fixing block is provided with a sterile iron wire, the inner side of the sterile iron wire is provided with a sample, and the inside of the beaker is provided with a sterile float. Through the limiting mechanism, the limiting block clamps or releases the sterile iron wire inserted into the inside of the fixing block, the sterile iron wire is prevented from deviating or shaking due to the fluid disturbance of the rotating field in the beaker, the sample at the lower end of the iron wire is ensured to be stably located in the middle of the cleaning liquid, and the uniform contact of the sample and the sterilization medium is ensured.

Description

Technical Field

[0001] This invention relates to the field of microbial sterilization technology, specifically to a microbial vortex sterilization device and a flow rate measurement method. Background Technology

[0002] Microorganisms are a group of organisms that are tiny and simple in structure, requiring a microscope to observe. They include various types such as bacteria, fungi, viruses, actinomycetes, and mycoplasma. They are widely distributed in soil, water, air, and various media in nature. Because microorganisms are tiny and easily dispersed in the treatment solution, ordinary static sterilization methods are prone to problems such as insufficient contact of the sterilization medium and the existence of sterilization dead zones. In addition, some microorganisms have high requirements for the uniformity of the contact environment. Therefore, a microbial vortex sterilization device is needed.

[0003] The microbial swirl field sterilization device is a specialized device adapted to microbial sterilization scenarios. In existing technologies, sterile iron wires are usually hung directly on the outer wall of the beaker. When the magnetic stirrer drives the sterile rotor to form a swirl field, the cleaning liquid will generate continuous fluid disturbance. This disturbance can easily cause the sterile iron wire to deviate radially or axially, thereby causing the sample fixed at the lower end of the iron wire to deviate from the preset sterilization core area, thus affecting the use of the sterilization device. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a microbial vortex sterilization device and flow rate measurement method, which solves the problem that when a sterile wire is directly hung on the outer wall of a beaker, the sample fixed at the lower end of the wire is prone to deviating from the preset sterilization core area.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a microbial vortex sterilization device, comprising a magnetic stirrer and a camera, characterized in that: a beaker is disposed on the upper surface of the magnetic stirrer, a sterile rotor is disposed inside the beaker, an arc-shaped frame and a fixed frame are respectively disposed on the outer wall of the beaker, a threaded rod is rotatably connected inside the fixed frame, the outer wall of the threaded rod is threadedly connected to the inside of the arc-shaped frame, a fixed block is fixedly connected to the upper surface of the fixed frame, a sterile iron wire is disposed inside the fixed block, a sample is disposed on the inner side of the sterile iron wire, a sterile float is disposed inside the beaker, a support frame is installed on the outer wall of the camera, a base is inserted into the outer wall of the support frame, a limiting mechanism is disposed on the outer wall of the sterile iron wire, the limiting mechanism includes a limiting block, the outer wall of the limiting block is disposed on the outer wall of the sterile iron wire, and the outer wall of the limiting block is slidably connected to the inside of the fixed block.

[0006] The above scheme utilizes a magnetic stirrer as the core power and temperature control component to drive a sterile rotor to rotate, creating a swirling field while maintaining a stable temperature of the treatment solution inside the beaker. The beaker is used to hold the treatment solution, providing a swirling field environment for microbial sterilization. A sterile wire is used to fix the sample, which is then stabilized in the middle of the treatment solution by a limiting mechanism, an arc-shaped frame, a fixing frame, and their connecting components, ensuring that the sample is in the core area of ​​the swirling field. The camera, located 20 centimeters above the sample via a base and support frame, facilitates the capture of the movement trajectory of sterile floats on the liquid surface, providing image data for subsequent analysis and calculation of the water flow angular velocity and linear velocity.

[0007] Preferably, an L-shaped frame is fixedly connected to the outer wall of the limiting block, the outer wall of the L-shaped frame is slidably connected to the fixed block, a fixed rod is slidably connected inside the L-shaped frame, and the outer wall of the fixed rod is fixedly connected to the outer wall of the fixed frame.

[0008] Preferably, the outer wall of the L-shaped frame is provided with a transmission cylinder, and the outer wall of the L-shaped frame is slidably connected to the inner wall of the transmission cylinder with a threaded groove on its surface. The inner wall of the transmission cylinder is rotatably connected to the outer wall of the threaded rod, and a limit member is provided between the transmission cylinder and the fixed frame.

[0009] Preferably, the threaded rod is provided with a moving mechanism, which includes a moving rod. The bottom end of the moving rod is slidably connected to the inside of the threaded rod, and a trapezoidal frame is fixedly connected to the lower surface of the moving rod.

[0010] Preferably, the outer wall of the trapezoidal frame penetrates the interior of the threaded rod and is slidably connected to the interior of the transmission cylinder, and a spring is fixedly connected between the trapezoidal frame and the threaded rod.

[0011] Preferably, the trapezoidal frame is internally slidably connected to a slide rod, and the bottom end of the slide rod is fixedly connected to the inside of the threaded rod.

[0012] Preferably, a limiting rod is slidably connected inside the base, and one end of a second spring is fixedly connected to the outer wall of the limiting rod, while the other end of the second spring is fixedly connected inside the base.

[0013] Preferably, the outer wall of the limiting rod is inserted into the inside of the support frame, and a push plate is fixedly connected to the outer wall of the limiting rod. The outer wall of the push plate is slidably connected to the inside of the base.

[0014] Preferably, a movable plate is fixedly connected to the outer wall of the push plate, and the outer wall of the movable plate is slidably connected to the inside of the base.

[0015] Preferably, a method for determining the flow rate of a microbial vortex sterilization device, used in a microbial vortex sterilization device, the method comprising the following steps:

[0016] S1. Place the beaker containing the treatment liquid on a magnetic stirrer, put a sterile rotor that has been sterilized by UV irradiation into the beaker, and place a sterile float on the surface of the treatment liquid to ensure that the sterile float can move freely with the liquid swirling.

[0017] S2. Adjust the rotor speed of the magnetic stirrer by turning the knob. After setting the target speed, let it stand for a period of time until the magnetic stirrer speed stabilizes to ensure that a stable swirling field is formed in the beaker. At this time, the movement of the sterile float tends to be regular.

[0018] S3. Using a limiting mechanism, the sample to be sterilized is fixed in the middle of the treatment liquid in the beaker by a sterile wire to prevent the sample from obscuring the sterile float. The camera is fixed 20cm above the beaker by the support frame and base. The camera is adjusted to ensure that it can completely capture the movement trajectory of the sterile float on the surface of the treatment liquid.

[0019] S4. Activate the camera to continuously capture the motion of the sterile float in the stable vortex field, record the complete motion trajectory of the sterile float within a set time period, analyze the motion trajectory image, extract the motion path of the sterile float per unit time, combine the circular trajectory of the sterile float to calculate the angular velocity of the water flow at different rotor speeds, and further calculate the linear velocity of the water flow based on the radius of the sterile float's motion trajectory using the formula: linear velocity = angular velocity × trajectory radius.

[0020] This invention provides a microbial vortex sterilization device and a flow rate measurement method. It has the following beneficial effects:

[0021] 1. The present invention uses a limiting mechanism to clamp or release the sterile iron wire inserted into the fixing block, thereby preventing the sterile iron wire from shifting or shaking due to fluid disturbance in the swirling field inside the beaker, ensuring that the sample at the lower end of the iron wire is stably in the middle of the cleaning solution, and ensuring uniform contact between the sample and the sterilization medium.

[0022] 2. This invention controls the movement of the arc-shaped frame towards the fixed frame or towards the outside of the beaker by rotating the threaded rod, thereby adjusting the tightness of the arc-shaped frame and the fixed frame on the beaker to adjust the depth of the sample in the treatment solution. It can also accommodate beakers of different sizes within the range of the arc-shaped frame and the fixed frame, improving the adaptability of the device to different experimental scenarios.

[0023] 3. This invention allows the thumb and middle finger to extend and push the push plates on both sides, which in turn causes the limiting rods on both sides to slide out of the support frame. This facilitates the adjustment of the camera's height relative to the sample. The vertical distance between the camera and the sample can be flexibly adjusted according to changes in the height of the beaker or the volume of the liquid being processed, ensuring complete coverage of the float's movement area and improving the ease of operation. Attached Figure Description

[0024] Figure 1 This is a three-dimensional schematic diagram of the present invention;

[0025] Figure 2 This is a partial structural diagram of the movable block of the present invention;

[0026] Figure 3 This is a cross-sectional view of the internal structure of the fixing block of the present invention;

[0027] Figure 4 This is a cross-sectional view of the internal structure of the threaded rod of the present invention;

[0028] Figure 5 This is a partial structural diagram of the push plate of the present invention;

[0029] Figure 6 This is a cross-sectional view of the internal structure of the base of the present invention.

[0030] The components are as follows: 1. Magnetic stirrer; 2. Beaker; 3. Arc-shaped frame; 4. Fixed frame; 5. Threaded rod; 6. Fixed block; 7. Sterile wire; 8. Sample; 9. Sterile rotor; 10. Base; 11. Support frame; 12. Camera; 13. Sterile float; 14. Limiting mechanism; 141. Transmission cylinder; 142. Threaded groove; 143. Limiting component; 144. L-shaped frame; 145. Fixed rod; 146. Limiting block; 15. Moving mechanism; 151. Moving rod; 152. Trapezoidal frame; 153. Spring 1; 154. Slide rod; 16. Limiting rod; 17. Spring 2; 18. Push plate; 19. Moving plate. Detailed Implementation

[0031] The technical solution of the present invention will now be clearly and completely described with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0032] Please see the appendix Figure 1 - Appendix Figure 3This invention provides a microbial vortex sterilization device, including a magnetic stirrer 1 and a camera 12. A beaker 2 is disposed on the upper surface of the magnetic stirrer 1, and a sterile rotor 9 is disposed inside the beaker 2. An arc-shaped frame 3 and a fixed frame 4 are respectively disposed on the outer wall of the beaker 2. A threaded rod 5 is rotatably connected inside the fixed frame 4, and the outer wall of the threaded rod 5 is threadedly connected to the inside of the arc-shaped frame 3. A fixed block 6 is fixedly connected to the upper surface of the fixed frame 4, and a sterile iron wire 7 is disposed inside the fixed block 6. A sample 8 is disposed on the inner side of the sterile iron wire 7. A sterile float 13 is disposed inside the beaker 2. A support frame 11 is installed on the outer wall of the camera 12, and a base 10 is inserted into the outer wall of the support frame 11. A limiting mechanism 14 is disposed on the outer wall of the sterile iron wire 7. The limiting mechanism 14 includes a limiting block 146, and the outer wall of the limiting block 146 is disposed on the outer wall of the sterile iron wire 7. The outer wall of the limiting block 146 is slidably connected to the inside of the fixed block 6.

[0033] Specifically, the arc-shaped frame 3 and the fixed frame 4 are identical in shape, both being arc-shaped. Furthermore, the sides of both the arc-shaped frame 3 and the fixed frame 4 closest to the beaker 2 are made of elastic material. This allows the arc-shaped frame 3 to be pulled towards the fixed frame 4 by the rotation of the threaded rod 5. Stress is generated between the beaker 2, the arc-shaped frame 3, and the fixed frame 4, causing deformation of the arc-shaped frame 3 and the fixed frame 4 for better fixation to the outer wall of the beaker 2. The threaded rod 5 controls the movement of the arc-shaped frame 3 towards the fixed frame 4 or towards the outside of the beaker 2, thus loosening the arc-shaped frame 3 and the fixed frame 4 from the beaker 2. The clamping mechanism 14 is used to clamp or release the sterile wire 7 inserted into the fixing block 6, thereby fixing the position of the sterile wire 7 and the sample 8 inside the beaker 2 and adjusting the height of the sterile wire 7 and the sample 8 inside the beaker 2 to meet the actual operation requirements. The limiting mechanism 14 clamps or releases the sterile wire 7 inserted into the fixing block 6, which makes it easier to fix the position of the sterile wire 7 and the sample 8 and prevents the sterile wire 7 from shifting or shaking due to the fluid disturbance of the swirling field inside the beaker 2. The sterile float 13 floats on the liquid surface and moves with the swirling flow. Its movement trajectory can be used as the basis for calculating the angular velocity and linear velocity of the water flow.

[0034] Please see the appendix Figure 3 An L-shaped frame 144 is fixedly connected to the outer wall of the limiting block 146. The outer wall of the L-shaped frame 144 is slidably connected to the fixing block 6. A fixing rod 145 is slidably connected inside the L-shaped frame 144. The outer wall of the fixing rod 145 is fixedly connected to the outer wall of the fixing frame 4. A transmission cylinder 141 is provided on the outer wall of the L-shaped frame 144. The outer wall of the L-shaped frame 144 is slidably connected to the inner wall of the transmission cylinder 141 with a threaded groove 142. The inner wall of the transmission cylinder 141 is rotatably connected to the outer wall of the threaded rod 5. A limiting member 143 is provided between the transmission cylinder 141 and the fixing frame 4.

[0035] Specifically, the transmission cylinder 141 rotates on the outer wall of the threaded rod 5, allowing the transmission cylinder 141 and the threaded rod 5 to rotate independently. The transmission cylinder 141 is limited by the limiting member 143 to prevent accidental rotation. The L-shaped frame 144 and the limiting block 146 move to the right through the threaded groove 142. The threaded rod 5 is fixed to the transmission cylinder 141 by internal components and is also limited by the self-locking of the limiting member 143. With the opening of the threaded groove 142, as the transmission cylinder 141 rotates backward, the L-shaped frame 144 and the limiting block 146 move towards the wire. The fixing rod 145 ensures the smooth movement of the L-shaped frame 144. The movement of the L-shaped frame 144 and the limiting block 146 is limited by the inside of the fixing block 6. The limiting member 143 is composed of a ratchet, a pawl, and an elastic element. The short rod set by the fixing frame 4 supports the rotation of the ratchet and pawl to self-lock the rotation of the transmission cylinder 141.

[0036] Please see the appendix Figure 3 - Appendix Figure 4 The threaded rod 5 is provided with a moving mechanism 15, which includes a moving rod 151. The bottom end of the moving rod 151 is slidably connected to the inside of the threaded rod 5. A trapezoidal frame 152 is fixedly connected to the lower surface of the moving rod 151. The outer wall of the trapezoidal frame 152 penetrates the inside of the threaded rod 5 and is slidably connected to the inside of the transmission cylinder 141. A spring 153 is fixedly connected between the trapezoidal frame 152 and the threaded rod 5. A sliding rod 154 is slidably connected inside the trapezoidal frame 152. The bottom end of the sliding rod 154 is fixedly connected to the inside of the threaded rod 5.

[0037] Specifically, the movable rod 151 facilitates the transmission of the trapezoidal frame 152, allowing it to slide in and out of the transmission cylinder 141, thus keeping the transmission cylinder 141 and the threaded rod 5 fixed. The movement of the movable rod 151 and the trapezoidal frame 152 is limited by the internal structure of the threaded rod 5, while the sliding rod 154 limits the movement of the trapezoidal frame 152. The spring 153 provides support for the trapezoidal frame 152 when it is initially located inside the transmission cylinder 141, and also provides a restoring force after the trapezoidal frame 152 moves towards the center of the threaded rod 5.

[0038] Please see the appendix Figure 5 - Appendix Figure 6 A limiting rod 16 is slidably connected inside the base 10. One end of a second spring 17 is fixedly connected to the outer wall of the limiting rod 16, and the other end of the second spring 17 is fixedly connected inside the base 10. The outer wall of the limiting rod 16 is inserted into the support frame 11. A push plate 18 is fixedly connected to the outer wall of the limiting rod 16, and the outer wall of the push plate 18 is slidably connected inside the base 10. A movable plate 19 is fixedly connected to the outer wall of the push plate 18, and the outer wall of the movable plate 19 is slidably connected inside the base 10.

[0039] Specifically, by Figure 5 It is known that the support frame 11 has multiple grooves inside, arranged at equal intervals, which match the outer wall of the limiting rod 16. The limiting rod 16 slides in and out inside the support frame 11, which facilitates limiting or releasing the support frame 11, thereby facilitating the adjustment of the height of the support frame 11 and its connecting parts. The second spring 17 provides the initial supporting force for limiting the support frame 11 to the limiting rod 16. The movement of the limiting rod 16, the push plate 18 and the moving plate 19 are offset and limited by the interior of the base 10. The moving plate 19 moves with the push plate 18, which helps to protect the movement of the limiting rod 16 and the second spring 17, and avoids the movement of the limiting rod 16 being affected by foreign objects.

[0040] Please see the appendix Figure 1 - Appendix Figure 6 A method for determining the flow rate of a microbial vortex sterilization device, used in a microbial vortex sterilization device, the method comprising the following steps:

[0041] S1. Place the beaker 2 containing the treatment liquid on the magnetic stirrer 1, put the sterile rotor 9, which has been sterilized by ultraviolet light irradiation, into the beaker 2, and at the same time place the sterile float 13 on the surface of the treatment liquid to ensure that the sterile float 13 can move freely with the liquid swirling.

[0042] S2. Adjust the rotor speed by turning the knob of the magnetic stirrer 1. After setting the target speed, let it stand for a period of time until the speed of the magnetic stirrer 1 stabilizes, ensuring that a stable swirling field is formed in the beaker 2. At this time, the movement state of the sterile float 13 tends to be regular.

[0043] S3. Through the limiting mechanism 14, the sample 8 to be sterilized is fixed in the middle of the treatment liquid in the beaker 2 by the sterile wire 7 to avoid the sample 8 from blocking the sterile float 13. The camera 12 is fixed 20cm above the beaker 2 by the support frame 11 and the base 10. The camera 12 is adjusted to ensure that it can completely capture the movement trajectory of the sterile float 13 on the surface of the treatment liquid.

[0044] S4. Start the camera 12 to continuously capture the motion process of the sterile float 13 in the stable vortex field, record the complete motion trajectory of the sterile float 13 within a set time period, analyze the motion trajectory image, extract the motion path of the sterile float 13 in a unit time, combine the circular trajectory of the sterile float 13 to calculate the angular velocity of the water flow at different rotor speeds, and further calculate the linear velocity of the water flow based on the radius of the motion trajectory of the sterile float 13 by using the formula: linear velocity = angular velocity × trajectory radius.

[0045] Workflow: When using this device, place the beaker 2 containing the treatment liquid on the magnetic stirrer 1, and put the sterile rotor 9, which has been irradiated by ultraviolet light, into the beaker 2. Then place the sterile float 13 on the surface of the liquid. The magnetic stirrer 1 drives the sterile rotor 9 to rotate, thereby rotating the liquid. Adjust the speed by using the knob on the magnetic stirrer 1. After the speed stabilizes, place the sample 8 into the liquid.

[0046] First, the sample 8 is assembled using sterile wire 7. Then, the end of sterile wire 7 furthest from the sample 8 is inserted into the fixing block 6. By rotating the transmission cylinder 141, the L-shaped frame 144, driven by the threaded groove 142, moves the limiting block 146 to limit the sterile wire 7. The fixing rod 145 limits the rotation of the L-shaped frame 144, and the limiting member 143 self-locks the rotation of the transmission cylinder 141. After the sterile wire 7 is fixed, the moving rod 151 is pressed, causing the trapezoidal frame 152 to move downward under the limitation of the sliding rod 154, compressing the spring 153. The trapezoidal frame 152 then slides out of the transmission cylinder 141. The internal structure allows for easy rotation of the threaded rod 5. By adjusting the forward and reverse rotation of the threaded rod 5, the distance between the arc frame 3 and the fixed frame 4 can be adjusted. First, the arc frame 3 is moved outward so that the interior of the arc frame 3 and the fixed frame 4 fits inside the outer wall of the beaker 2. Then, the arc frame 3 and the fixed frame 4 are moved up and down to ensure that the sample 8 is in the middle of the liquid. Then, the arc frame 3 is moved towards the fixed frame 4 via the threaded rod 5, thereby fixing the position of the arc frame 3, the fixed frame 4 and their connecting parts. The camera 12 is located on the same axis as the sample 8 and is located 20cm directly above the beaker 2, which facilitates the complete capture of the float's movement.

[0047] By positioning the thumb and middle finger between the two push plates 18, and extending the thumb and middle finger, the push plates 18 on both sides are pushed to move outwards from the base 10, thereby causing the limiting rods 16 on both sides to move outwards from the base 10, compressing the spring 17. The spring 17 then slides out of the support frame 11 through the limiting rods 16 and is no longer limited, making it convenient to adjust the height of the support frame 11 according to actual needs, thereby adjusting the distance between the camera 12 and the sample 8.

[0048] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A microbial vortex sterilization device, comprising a magnetic stirrer (1) and a camera (12), characterized in that: The upper surface of the magnetic stirrer (1) is provided with a beaker (2), and the inside of the beaker (2) is provided with a sterile rotor (9). The outer wall of the beaker (2) is provided with an arc-shaped frame (3) and a fixed frame (4). The fixed frame (4) is rotatably connected to a threaded rod (5), and the outer wall of the threaded rod (5) is threadedly connected to the inside of the arc-shaped frame (3). The upper surface of the fixed frame (4) is fixedly connected with a fixing block (6), and the inside of the fixing block (6) is provided with a sterile iron wire (7). A sample (8) is provided on the inner side. A sterile float (13) is provided inside the beaker (2). A support frame (11) is installed on the outer wall of the camera (12). A base (10) is inserted into the outer wall of the support frame (11). A limiting mechanism (14) is provided on the outer wall of the sterile wire (7). The limiting mechanism (14) includes a limiting block (146). The outer wall of the limiting block (146) is provided on the outer wall of the sterile wire (7). The outer wall of the limiting block (146) is slidably connected to the inside of the fixing block (6).

2. The microbial vortex sterilization device according to claim 1, characterized in that: The outer wall of the limiting block (146) is fixedly connected to an L-shaped frame (144), the outer wall of the L-shaped frame (144) is slidably connected to the fixing block (6), the inner wall of the L-shaped frame (144) is slidably connected to a fixing rod (145), and the outer wall of the fixing rod (145) is fixedly connected to the outer wall of the fixing frame (4).

3. The microbial vortex sterilization device according to claim 2, characterized in that: The outer wall of the L-shaped frame (144) is provided with a transmission cylinder (141), and the outer wall of the L-shaped frame (144) is slidably connected to the inner wall of the transmission cylinder (141) with a threaded groove (142) on its surface. The inner wall of the transmission cylinder (141) is rotatably connected to the outer wall of the threaded rod (5). A limit member (143) is provided between the transmission cylinder (141) and the fixed frame (4).

4. The microbial vortex sterilization device according to claim 1, characterized in that: The threaded rod (5) is provided with a moving mechanism (15), which includes a moving rod (151). The bottom end of the moving rod (151) is slidably connected to the inside of the threaded rod (5), and a trapezoidal frame (152) is fixedly connected to the lower surface of the moving rod (151).

5. A microbial vortex sterilization device according to claim 4, characterized in that: The outer wall of the trapezoidal frame (152) penetrates the interior of the threaded rod (5) and is slidably connected to the interior of the transmission cylinder (141). A spring (153) is fixedly connected between the trapezoidal frame (152) and the threaded rod (5).

6. The microbial vortex sterilization device according to claim 5, characterized in that: The trapezoidal frame (152) is internally slidably connected to a slide rod (154), and the bottom end of the slide rod (154) is fixedly connected to the inside of the threaded rod (5).

7. The microbial vortex sterilization device according to claim 1, characterized in that: The base (10) is internally connected to a limiting rod (16), and one end of a second spring (17) is fixedly connected to the outer wall of the limiting rod (16). The other end of the second spring (17) is fixedly connected to the inside of the base (10).

8. A microbial vortex sterilization device according to claim 7, characterized in that: The outer wall of the limiting rod (16) is inserted into the inside of the support frame (11), and the outer wall of the limiting rod (16) is fixedly connected to the push plate (18), and the outer wall of the push plate (18) is slidably connected to the inside of the base (10).

9. A microbial vortex sterilization device according to claim 8, characterized in that: The outer wall of the push plate (18) is fixedly connected to a movable plate (19), and the outer wall of the movable plate (19) is slidably connected to the inside of the base (10).

10. A method for determining the flow rate of a microbial vortex sterilization device, characterized in that, The method for a microbial vortex sterilization device according to any one of claims 1-9 comprises the following steps: S1. Place the beaker (2) containing the treatment liquid on the magnetic stirrer (1), put the sterile rotor (9) that has been sterilized by ultraviolet light into the beaker (2), and place the sterile float (13) on the surface of the treatment liquid to ensure that the sterile float (13) can move freely with the liquid swirling. S2. Adjust the rotor speed by turning the knob of the magnetic stirrer (1), set the target speed, and let it stand for a period of time until the speed of the magnetic stirrer (1) is stable, so as to ensure that a stable swirling field is formed in the beaker (2). At this time, the movement state of the sterile float (13) tends to be regular. S3. Using the limiting mechanism (14), the sample (8) to be sterilized is fixed in the middle of the treatment liquid in the beaker (2) by the sterile wire (7) to avoid the sample (8) from blocking the sterile float (13). The camera (12) is fixed 20cm above the beaker (2) by the support frame (11) and the base (10). The camera (12) is adjusted to ensure that it can completely capture the movement trajectory of the sterile float (13) on the surface of the treatment liquid. S4. Start the camera (12) to continuously capture the motion process of the sterile float (13) in the stable vortex field, record the complete motion trajectory of the sterile float (13) within a set time period, analyze the motion trajectory image, extract the motion path of the sterile float (13) in a unit time, combine the circular trajectory of the sterile float (13) to calculate the angular velocity of the water flow at different rotor speeds, and further calculate the linear velocity of the water flow based on the radius of the motion trajectory of the sterile float (13) by using linear velocity = angular velocity × trajectory radius.

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