A fusion cholesterol oxidase and its use in a biosensor element
By designing a fusion protein of cholesterol oxidase and carbohydrate binding module (CBM), the problem of weak binding between the enzyme and cellulose was solved, thus improving the stability and detection accuracy of the biosensor.
CN122103365APending Publication Date: 2026-05-29BIOLOGY INST OF SHANDONG ACAD OF SCI
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Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- BIOLOGY INST OF SHANDONG ACAD OF SCI
- Filing Date
- 2026-01-21
- Publication Date
- 2026-05-29
AI Technical Summary
Technical Problem
Existing technologies for immobilized cholesterol oxidase biosensors suffer from problems such as weak binding between the enzyme and cellulose, and unstable structure and function, which affect the accuracy and stability of detection.
Method used
A biosensing element is formed by using a cholesterol oxidase-carbohydrate binding module (CBM) fusion protein, which enhances the binding ability of CBM to cellulose by fusing CBM with ChOx at the N-terminus or C-terminus, and then immobilizing it on the electrode surface.
Benefits of technology
It achieves more stable electrochemical performance and anti-interference ability, improving the accuracy and stability of cholesterol detection.
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Figure CN122103365A_ABST
Abstract
The present application constructs a fusion protein by utilizing the specific affinity adsorption property of a carbohydrate binding module (CBM) to cellulose, the fusion protein comprising a cholesteric oxidase and a carbohydrate binding module (CBM), and then uses nanocellulose membranes as the immobilization carrier of the fusion protein for preparing a biosensor element, and the obtained biosensor element can exhibit good catalytic properties and detection stability.
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