A heparin sodium extraction device and extraction method

Abstract

The present application relates to heparin sodium preparation technical field, specifically to a kind of heparin sodium extraction equipment and extraction method, including extraction tank, stirring shaft, two stirring mechanisms and two telescopic mechanisms;Stirring shaft can rotate around the central axis of extraction tank;Two stirring mechanisms are symmetrically distributed in the two sides of stirring shaft;Two telescopic mechanisms correspond to two stirring mechanisms one by one, for driving stirring mechanism to retract along the radial direction of extraction tank, to make stirring mechanism and the inside wall of extraction tank be attached or be separated;In enzymolysis stage, stirring mechanism and the inside wall of extraction tank keep reasonable interval, avoid high viscosity small intestinal mucosa and tank wall friction to generate additional resistance;In cleaning stage, stirring mechanism is attached tank wall, and rotates along with stirring shaft circumferentially, without additional extension external scraping tool, can be continuously scraped along tank wall full circumference.

Description

Technical Field

[0001] This invention relates to the field of heparin sodium preparation technology, specifically to a heparin sodium extraction device and extraction method. Background Technology

[0002] Sodium heparin is a widely used acidic mucopolysaccharide anticoagulant drug with irreplaceable application value in medical scenarios such as thrombosis prevention, hemodialysis, and extracorporeal circulation. Its industrial production mainly uses the small intestinal mucosa of livestock such as pigs and cattle as raw materials and is prepared through biological enzymatic hydrolysis process.

[0003] To ensure sufficient contact between the intestinal mucosal erosion and the protease and to improve the efficiency of the enzymatic hydrolysis reaction, existing enzymatic extraction tanks are equipped with a stirring mechanism to achieve uniform mixing of the liquid. Currently, a constant distance is generally maintained between the stirring mechanism and the inner wall of the extraction tank. When the enzymatic hydrolysis process is completed and it is necessary to clean the mucosal residue adhering to the inner wall of the tank, the liquid in the tank must be completely drained and the equipment stopped before the operator can insert an external scraping tool from the tank opening into the gap between the stirring mechanism and the tank wall to complete the scraping and cleaning.

[0004] However, once the external scraping tool is inserted into the tank, it is obstructed by components such as the stirring shaft and blades, preventing continuous circumferential movement along the tank wall. This creates blind spots in the obstructed areas, hindering comprehensive circumferential cleaning of the tank wall. Furthermore, manual operation requires repeated adjustments to the scraping tool's insertion angle and depth, resulting in a cumbersome and time-consuming process that significantly extends equipment downtime and reduces the efficiency of continuous operation in the industrial production of heparin sodium. Therefore, we propose a heparin sodium extraction device and method to effectively address these shortcomings. Summary of the Invention

[0005] The purpose of this invention is to provide a heparin sodium extraction device and extraction method to solve the problem mentioned in the background art of the difficulty in cleaning the tank wall in the entire circumference.

[0006] This invention is achieved through the following technical solution: a heparin sodium extraction device, comprising an extraction tank, and further comprising: The stirring shaft is arranged along the central axis of the extraction tank and can rotate around the central axis of the extraction tank; Two stirring mechanisms are symmetrically distributed on both sides of the stirring shaft; the extension direction of each stirring mechanism is parallel to the axial direction of the stirring shaft. Two telescopic mechanisms correspond one-to-one with two stirring mechanisms; one end of each telescopic mechanism is fixedly connected to the stirring shaft, and the other end is fixedly connected to the corresponding stirring mechanism, which is used to drive the stirring mechanism to extend and retract radially along the extraction tank so that the stirring mechanism is in contact with or separates from the inner wall of the extraction tank.

[0007] In one embodiment, a rotary drive mechanism is fixedly installed on the outer top of the extraction tank. The output end of the rotary drive mechanism is connected to the stirring shaft for driving the stirring shaft to rotate around the central axis of the extraction tank.

[0008] In one embodiment, the stirring mechanism includes a C-shaped frame fixedly connected to one end of the telescopic mechanism, the opening of the C-shaped frame being disposed away from the stirring shaft; a rotating shaft is rotatably connected inside the C-shaped frame, the rotating shaft being able to rotate around its own axis; a stirring blade is fixedly sleeved on the rotating shaft, the length direction of the stirring blade being parallel to the axial direction of the stirring shaft; when the stirring blade is in contact with the inner wall of the extraction tank, the width direction of the stirring blade is parallel to the radial direction of the extraction tank; when the stirring blade is separated from the inner wall of the extraction tank, the width direction of the stirring blade forms a preset angle with the radial direction of the extraction tank.

[0009] In one embodiment, the width direction of the stirring blade is at a preset angle of 15° to 60° with the radial direction of the extraction tank.

[0010] In one embodiment, gears are fixedly sleeved at both the upper and lower ends of the rotating shaft, and connecting frames corresponding to each gear are fixedly connected to the side wall of the stirring shaft. A rack adapted to the corresponding gear is fixedly connected to each connecting frame. The extension direction of the rack is the same as the extension direction of the C-shaped frame. When the C-shaped frame extends or retracts radially along the extraction tank, the rotating shaft is driven to rotate around its own axis through the cooperation of the rack and gear.

[0011] In one embodiment, the upper and lower ends of the C-shaped frame are fixedly connected to housings, and the end of the rotating shaft is movably inserted into the corresponding housing; each housing is provided with a torsion spring, one torsion arm of the torsion spring is connected to the inner wall of the housing, and the other torsion arm is connected to the shaft body of the rotating shaft; when the gear and rack disengage, the elastic torque of the torsion spring drives the rotating shaft to rotate around its own axis, so that the width direction of the stirring blades forms a preset angle with the radial direction of the extraction tank.

[0012] In one embodiment, the telescopic mechanism includes a plurality of sleeves fixed parallel and spaced apart to the side wall of the stirring shaft, each sleeve extending radially along the extraction tank; a slider is slidably connected inside each sleeve, the slider being movable along the axial direction of the sleeve; a connecting rod is fixedly connected between the slider and the corresponding stirring mechanism, the connecting rod movably passing through the end wall of the corresponding sleeve.

[0013] In one embodiment, the stirring shaft is a hollow shaft, and a linkage shaft is movably inserted inside the stirring shaft. The linkage shaft is capable of moving along the axial direction of the stirring shaft. A linkage rod corresponding to each slider is hinged on the linkage shaft, and the end of each linkage rod away from the linkage shaft is hinged to the corresponding slider. When the linkage shaft moves along the axial direction of the stirring shaft, the slider is driven to move along the axial direction of the sleeve through the linkage rod.

[0014] In one embodiment, the lower end of the linkage shaft extends to the outside of the stirring shaft, and an auxiliary scraper is fixedly installed at the lower end of the linkage shaft. The outline of the auxiliary scraper is adapted to the inner bottom wall of the extraction tank. When the stirring mechanism is separated from the inner side wall of the extraction tank, the auxiliary scraper is separated from the inner bottom wall of the extraction tank. When the stirring mechanism is in contact with the inner side wall of the extraction tank, the auxiliary scraper is in contact with the inner bottom wall of the extraction tank.

[0015] The present invention also provides an extraction method applicable to the heparin sodium extraction equipment described above, comprising the following steps: Step 1: Put the small intestinal mucosal erosion into the extraction tank, adjust the pH of the solution to the range suitable for enzymatic hydrolysis, and heat to the enzymatic hydrolysis temperature; Step 2: Add protease solution into the extraction tank and simultaneously drive the stirring shaft to rotate; at this time, the telescopic mechanism is in the retracted state to separate the stirring mechanism from the inner wall of the extraction tank, and the material solution is stirred and mixed by the stirring mechanism; Step 3: Keep the mixture warm and stir until the protein in the solution is fully hydrolyzed and the heparin is completely dissociated and released; Step 4: After draining the liquid from the extraction tank, extend the telescopic mechanism to make the stirring mechanism fit against the inner wall of the extraction tank, and use the stirring mechanism to scrape and clean the inner wall of the extraction tank.

[0016] Compared with the prior art, the present invention provides a heparin sodium extraction device and extraction method, which has the following beneficial effects: 1. The stirring mechanism of the present invention can move radially along the extraction tank. During the enzymatic hydrolysis stage, the stirring mechanism maintains a reasonable distance from the inner wall of the extraction tank to avoid friction between the high-viscosity small intestinal mucosal erosion and the tank wall, thereby avoiding additional resistance. At the same time, it provides sufficient space for the flow of the liquid, ensuring uniform contact between the enzyme and the mucosal erosion. During the cleaning stage, the stirring mechanism rotates circumferentially with the stirring shaft after adhering to the tank wall. It can continuously scrape along the entire circumference of the tank wall without the need for additional external scraping tools. This effectively solves the technical problems of traditional external cleaning tools being obstructed by the structure, cumbersome operation, and incomplete cleaning, and significantly shortens the equipment downtime.

[0017] 2. The stirring blades of this invention can rotate around the axis of rotation. During the enzymatic hydrolysis stage, the width direction of the stirring blades forms a preset angle of 15° to 60° with the radial direction of the extraction tank. This tilted posture can significantly reduce the frontal impact resistance of the highly viscous liquid (small intestinal mucosal erosion) on the blades. During the cleaning stage, the stirring blades switch to a posture parallel to the radial direction of the tank wall, with the blade edges fully adhering to the tank wall, maximizing the scraping contact area and efficiently peeling off the mucosal residue adhering to the tank wall.

[0018] 3. This invention adopts an integrated design of axial lifting drive telescopic mechanism with linkage shaft, eliminating the need for additional independent telescopic drive components. Through the mechanical linkage between linkage shaft and telescopic mechanism, axial movement is converted into radial extension and contraction, which saves installation space inside the extraction tank, simplifies the transmission structure, and is suitable for the harsh working environment of hot, humid and viscous extraction tank.

[0019] 4. In this invention, an auxiliary scraper is added to the lower end of the linkage shaft. During the enzymatic hydrolysis stage, the auxiliary scraper separates from the bottom of the tank to avoid interfering with the axial circulation of the liquid and the flow of the bottom material. During the cleaning stage, the auxiliary scraper moves down with the linkage shaft to fit the bottom of the tank and rotates synchronously with the stirring shaft to scrape, effectively removing the mucous residue that is easy to remain at the bottom of the tank. Attached Figure Description

[0020] Figure 1 This is a schematic diagram of the enzymatic hydrolysis stage of the present invention; Figure 2 This is a schematic diagram of the cleaning stage of the present invention; Figure 3 This is a schematic diagram of the stirring shaft of the present invention; Figure 4 for Figure 3 Enlarged view of point A in the middle; Figure 5 This is a schematic diagram of the interior of the stirring shaft of the present invention; Figure 6 for Figure 5 Enlarged view of point B in the middle; Figure 7 for Figure 5 Enlarged view of point C in the middle.

[0021] In the diagram: 1. Extraction tank; 2. Stirring shaft; 3. Stirring mechanism; 301. C-shaped frame; 302. Rotating shaft; 303. Stirring blades; 4. Telescopic mechanism; 401. Sleeve; 402. Slider; 403. Connecting rod; 5. Rotary drive mechanism; 6. Gear; 7. Connecting frame; 8. Rack; 9. Housing; 10. Torsion spring; 11. Linkage shaft; 12. Linkage rod; 13. Auxiliary scraper. Detailed Implementation

[0022] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0023] Example 1: Please refer to Figures 1 to 7A heparin sodium extraction device includes an extraction tank 1, which is a vertical cylindrical container. The tank is equipped with a heating mechanism (such as a jacketed heat exchanger with hot water / steam temperature control), an enzyme addition mechanism (such as a multi-point atomizing nozzle), a pH adjustment mechanism (such as an online pH sensor and an automatic alkali addition pipeline), and auxiliary detection mechanisms such as a level gauge and a temperature sensor. It is capable of extracting heparin sodium using an enzymatic hydrolysis method.

[0024] This embodiment also includes: a stirring shaft 2, two stirring mechanisms 3, and two telescopic mechanisms 4.

[0025] First, the stirring shaft 2 is arranged along the central axis of the extraction tank 1 and can rotate around the central axis of the extraction tank 1. The upper end of the stirring shaft 2 passes through the top end cover of the extraction tank 1 (achieving a rotary seal through a mechanical seal assembly to prevent leakage of the liquid and external contamination), and the lower end extends into the lower part of the tank body, ensuring that the stirring range covers the core reaction zone of the liquid in the tank.

[0026] In this embodiment, to achieve the rotation of the stirring shaft 2, a rotary drive mechanism 5 is fixedly installed on the top of the extraction tank 1. The output end of the rotary drive mechanism 5 is connected to the stirring shaft 2 for driving the stirring shaft 2 to rotate around the central axis of the extraction tank 1. Specifically, the rotary drive mechanism 5 consists of a motor, a reducer, and a coupling, and the stirring speed can be adjusted according to the viscosity of the liquid during the enzymatic hydrolysis stage.

[0027] Secondly, the two stirring mechanisms 3 are symmetrically distributed on both sides of the stirring shaft 2 (with an included angle of 180°). The extension direction of each stirring mechanism 3 is parallel to the axial direction of the stirring shaft 2, ensuring that the stirring range covers most of the extraction tank 1 and that no mixing dead zones are likely to occur.

[0028] In addition, the two telescopic mechanisms 4 correspond one-to-one with the two stirring mechanisms 3. One end of each telescopic mechanism 4 is fixedly connected to the stirring shaft 2, and the other end is fixedly connected to the corresponding stirring mechanism 3, which is used to drive the stirring mechanism 3 to extend and retract radially along the extraction tank 1 so that the stirring mechanism 3 is in contact with or separates from the inner wall of the extraction tank 1.

[0029] During the enzymatic hydrolysis stage: the telescopic mechanism 4 is in a contracted state, and the stirring mechanism 3 is pulled towards the stirring shaft 2 through mechanical transmission, so that the stirring mechanism 3 and the inner wall of the extraction tank 1 maintain a reasonable distance; then the rotary drive mechanism 5 is started, which drives the stirring shaft 2 and the two stirring mechanisms 3 to rotate synchronously in the circumferential direction, so as to achieve full contact and mixing of the liquid and the protease, and complete the enzymatic hydrolysis reaction of heparin dissociation.

[0030] During the cleaning stage: After the liquid in the tank is completely drained, the telescopic mechanism 4 is extended, and the stirring mechanism 3 is pushed towards the inner wall of the extraction tank 1 by mechanical thrust until the edge of the stirring mechanism 3 is in contact with the tank wall; keep the rotary drive mechanism 5 running (switching to low speed), and the stirring shaft 2 drives the stirring mechanism 3 in contact with the tank wall to rotate circumferentially, peeling off the mucous membrane residue, protein flocs and other substances adhering to the tank wall.

[0031] By adopting the above design, the enzymatic hydrolysis stirring and tank wall cleaning functions are integrated into the same structure through the linkage of the telescopic mechanism 4 and the stirring mechanism 3. No additional scraping tools are required, which simplifies the overall structural design of the extraction tank 1 and greatly reduces the space occupied by the equipment.

[0032] During the enzymatic hydrolysis stage, the stirring mechanism 3 is kept away from the tank wall and at a reasonable distance, which can avoid the additional resistance caused by direct friction between the high viscosity mucosal chyme and the tank wall; at the same time, it leaves enough space for the flow of the liquid, which significantly improves the contact efficiency between the enzyme and the mucosal chyme.

[0033] During the cleaning phase, the stirring mechanism 3 continuously scrapes the tank wall in a circumferential direction, eliminating the blind spots caused by the obstruction of the stirring structure that traditional external tools create. It also eliminates the need for manual operation inside the tank, avoiding the pollution risks and operational safety hazards associated with manual cleaning, and significantly reducing equipment downtime, thereby improving the efficiency of continuous industrial production.

[0034] The stirring mechanism 3 is described below: The stirring mechanism 3 includes a C-shaped frame 301 fixedly connected to one end of the telescopic mechanism 4, with the opening of the C-shaped frame 301 facing away from the stirring shaft 2. A rotating shaft 302 is rotatably connected inside the C-shaped frame 301, capable of rotating around its own axis, and the axis of the rotating shaft 302 is parallel to the axis of the stirring shaft 2. Stirring blades 303 are fixedly sleeved on the rotating shaft 302, with the length direction of the stirring blades 303 parallel to the axial direction of the stirring shaft 2, ensuring an effective contact area for stirring and scraping.

[0035] When the stirring blade 303 is in contact with the inner wall of the extraction tank 1 (cleaning stage), the width direction of the stirring blade 303 is parallel to the radial direction of the extraction tank 1. At this time, the edge of the blade can fully contact the tank wall, maximizing the scraping contact area and effectively peeling off the adhesive residue adhering to the tank wall.

[0036] When the stirring blade 303 separates from the inner wall of the extraction tank 1 (enzymatic hydrolysis stage), the width direction of the stirring blade 303 forms a preset angle with the radial direction of the extraction tank 1, which can reduce the frontal impact resistance of the high-viscosity liquid (small intestinal mucosal erosion) on the blade.

[0037] It is worth mentioning that the preset included angle is 15° to 60°. This angle changes the contact between the blade and the high-viscosity small intestinal mucosal erosion from frontal impact to inclined flow, which greatly reduces fluid resistance.

[0038] With the above design, the stirring blades 303 are in an inclined position during the enzymatic hydrolysis stage, which can significantly reduce stirring resistance. Specifically, high-viscosity intestinal mucosal erosion is a non-Newtonian fluid. If the blades are horizontal (parallel to the radial direction), the front of the blades will directly impact the liquid during rotation, generating huge fluid resistance, leading to a surge in motor load and excessive energy consumption. However, the 15° to 60° inclined position changes the contact mode between the blades and the liquid to "sloping surface guidance," with the liquid flowing along the inclined surface of the blades, avoiding direct impact. The stirring torque is reduced by 25% to 40%, and the motor energy consumption is reduced simultaneously, significantly reducing the operating cost of industrial production. Furthermore, the radial shear force and centrifugal force generated when the inclined blades rotate can effectively inhibit the adhesion and deposition of high-viscosity mucosal erosion on the blade surface and tank wall.

[0039] During the cleaning stage, the stirring blades 303 are in a horizontal position, which maximizes the scraping contact area. Specifically, in the horizontal position, the width direction of the stirring blades 303 is parallel to the radial direction of the tank wall, and the entire side of the blades is fully in contact with the tank wall; when rotating, the blades form a continuous circular scraping trajectory along the tank wall, with no blind spots in cleaning, and can thoroughly peel off the adhesive residue, protein flocs and other attachments adhering to the tank wall.

[0040] In order to achieve the rotation of the rotating shaft 302, gears 6 are fixedly sleeved at both the upper and lower ends of the rotating shaft 302. Connecting brackets 7 corresponding to each gear 6 are fixedly connected to the side wall of the stirring shaft 2. A rack 8 adapted to the corresponding gear 6 is fixedly connected to each connecting bracket 7. The extension direction of the rack 8 is the same as the extension direction of the C-shaped frame 301.

[0041] When the C-shaped frame 301 extends or retracts radially along the extraction tank 1, the gear 6 moves synchronously with the C-shaped frame and meshes with the fixed rack 8 for transmission. Thus, through the cooperation of the rack 8 and the gear 6, the rotating shaft 302 is driven to rotate around its own axis, thereby realizing the switching of the blade posture.

[0042] By adopting the above design, the radial extension and retraction motion of the stirring mechanism 3 and the attitude switching of the stirring blades 303 are linked by a purely mechanical rigid linkage, eliminating the need for additional independent drive components, electrical control units, or sensing components for blade attitude adjustment. The blade attitude switching is entirely dependent on the extension and retraction motion of the stirring mechanism 3. Operators only need to control the extension and retraction state of the extension and retraction mechanism 3 to simultaneously complete the dual switching of the stirring position and blade attitude, eliminating the need for step-by-step extension and retraction and angle adjustment operations. This significantly shortens the switching time between enzymatic hydrolysis and cleaning conditions, reduces equipment downtime, and meets the efficiency requirements of continuous industrial production of heparin sodium.

[0043] During the research and development process, it was discovered that if the blade posture is limited solely by the transmission of gear 6 and rack 8, the impact of the liquid material during the enzymatic hydrolysis stage may cause the rotating shaft 302 to rotate passively, resulting in rigid wear of the tooth grooves and teeth. To solve this problem, the following design was developed: Both ends of the C-shaped frame 301 are fixedly connected to housings 9. The end of the rotating shaft 302 is movably inserted into the corresponding housing 9 and rotates with the housing 9 through rolling bearings (a sealing ring is provided at the junction of the rotating shaft 302 and the housing 9 to prevent liquid from entering). A torsion spring 10 is provided in each housing 9. The torsion spring 10 is sleeved on the outside of the rotating shaft 302. One torsion arm of the torsion spring 10 is connected to the inner wall of the housing 9, and the other torsion arm is connected to the shaft body of the rotating shaft 302.

[0044] When gear 6 disengages from rack 8 (i.e., the stirring mechanism 3 is fully retracted and away from the tank wall), the elastic torque of torsion spring 10 drives rotating shaft 302 to rotate around its own axis, so that the width direction of stirring blade 303 forms a preset angle with the radial direction of extraction tank 1. At this time, the blade can generate slight elastic oscillation with the impact of the liquid, avoiding gear wear caused by rigid impact, and further reducing stirring resistance.

[0045] To prevent excessive blade rotation from affecting subsequent attitude switching, a limiting block is welded to the shaft of the rotating shaft 302, and two limiting bosses are welded to the corresponding positions on the inner wall of the housing 9. The limiting block can rotate in the area between the two bosses, thereby limiting the rotation range of the stirring blade 303 and ensuring that the blade can be reset to a horizontal state when the gear 6 and rack 8 mesh during the cleaning stage.

[0046] The following is a description of telescopic mechanism 4: The telescopic mechanism 4 includes several sleeves 401 (at least two) that are fixed parallel to and spaced apart on the side wall of the stirring shaft 2, each sleeve 401 extending radially along the extraction tank 1. A slider 402 is slidably connected within each sleeve 401, and the slider 402 is capable of moving axially along the sleeve 401. A connecting rod 403 is fixedly connected between the slider 402 and the corresponding stirring mechanism 3 (C-shaped frame 301), and the connecting rod 403 movably passes through the end wall of the corresponding sleeve 401 (a sealing ring is provided at the end wall to prevent liquid from entering).

[0047] With the above structure, when the slider 402 moves axially along the sleeve 401, the connecting rod 403 drives the C-shaped frame 301 to move radially synchronously, thereby realizing the separation and contact switching between the stirring mechanism 3 and the tank wall.

[0048] To facilitate the movement of the sliders 402, the stirring shaft 2 is a hollow shaft, which allows for the installation of other components. A linkage shaft 11 is movably installed within the stirring shaft 2. The linkage shaft 11 can move axially along the stirring shaft 2 and is protected by a guide key and keyway on the inner wall of the stirring shaft 2 to prevent circumferential rotation during sliding. Linkage rods 12, corresponding one-to-one with each slider 402, are hinged to the linkage shaft 11. The end of each linkage rod 12 furthest from the linkage shaft 11 is hinged to the corresponding slider 402.

[0049] When the linkage shaft 11 moves along the axial direction of the stirring shaft 2, the linkage rod 12 rotates around the hinge point, converting the axial motion into radial thrust / tension. The linkage rod 12 drives the slider 402 to move along the axial direction of the sleeve 401, thereby realizing the extension and retraction of the stirring mechanism 3.

[0050] With the above structure, during the enzymatic hydrolysis stage, the linkage shaft 11 is in a high position, and the linkage rod 12 is in an inclined position, which generates a continuous pulling force on the slider 402, pulling the slider 402 into the sleeve 401; the stirring mechanism 3 is in a contracted state, maintaining a reasonable distance from the inner wall of the extraction tank 1, which is suitable for low-resistance stirring requirements.

[0051] During the cleaning stage, the control linkage shaft 11 moves downward along the stirring shaft 2 to a low position. At this time, the linkage rod 12 rotates synchronously, generating a thrust on the slider 402 and pushing the slider 402 to the outside of the sleeve 401. The stirring mechanism 3 is in an extended state, and the stirring blades 303 are in contact with the tank wall to meet the scraping cleaning requirements.

[0052] The above design converts axial motion into radial extension and retraction power through the linkage mechanism of the linkage shaft 11 and linkage rod 12. This eliminates the need for an independent radial drive component (such as a pneumatic or hydraulic cylinder) for the stirring mechanism 3, relying solely on the built-in space of the stirring shaft 2 for transmission. This significantly saves effective reaction space within the extraction tank, simplifies the overall equipment structure, and reduces assembly complexity. Only the axial lifting and lowering of the linkage shaft 11 needs to be controlled to simultaneously complete the extension and retraction switching of all stirring mechanisms 3. The operation logic is simple, eliminating the need for step-by-step adjustment of individual stirring mechanisms 3.

[0053] It should be noted that the axial movement of the linkage shaft 11 can be achieved by manual drive. For example, handles are welded and fixed on both sides of the upper end near the end of the linkage shaft 11, and a long strip opening is opened on the side wall of the stirring shaft 2 at the corresponding position for the handles to pass through; several fixing bolts are inserted between the stirring shaft 2 and the linkage shaft 11. After the linkage shaft 11 moves to the target position, it is locked to the stirring shaft 2 by tightening the fixing bolts to prevent displacement during operation. The structure is simple, low-cost, and suitable for small-scale production.

[0054] It is worth mentioning that, in order to achieve synchronous cleaning of the tank bottom and tank wall, the lower end of the linkage shaft 11 extends to the outside of the stirring shaft 2 (a sealing ring is provided at the junction of the linkage shaft 11 and the stirring shaft 2 to prevent the liquid from entering the interior of the stirring shaft 2). An auxiliary scraper 13 is fixedly installed at the lower end of the linkage shaft 11, and the outline of the auxiliary scraper 13 is adapted to the inner bottom wall of the extraction tank 1.

[0055] When the stirring mechanism 3 separates from the inner wall of the extraction tank 1, the auxiliary scraper 13 separates from the inner bottom wall of the extraction tank 1.

[0056] When the stirring mechanism 3 is in contact with the inner wall of the extraction tank 1, the auxiliary scraper 13 is in contact with the inner bottom wall of the extraction tank 1.

[0057] With the above design, during the enzymatic hydrolysis stage, the linkage shaft 11 is in a high position, and the auxiliary scraper 13 maintains a certain distance from the bottom of the tank to avoid interfering with the flow of materials at the bottom and to prevent the mucus from depositing and clumping at the bottom of the tank.

[0058] During the cleaning stage, the linkage shaft 11 moves down to a low position, the auxiliary scraper 13 fits against the bottom of the tank, and rotates synchronously with the stirring shaft 2 to scrape and clean the remaining mucosal residue and protein flocs at the bottom of the tank.

[0059] Example 2: This example proposes an extraction method applicable to the heparin sodium extraction equipment of Example 1, comprising the following steps: Step 1: Put the small intestinal mucosal erosion into extraction tank 1, adjust the pH of the solution to the range suitable for enzymatic hydrolysis, and heat to the enzymatic hydrolysis temperature.

[0060] Step 2: Add protease solution to extraction tank 1, and simultaneously start the rotary drive mechanism 5 to drive the stirring shaft 2 to rotate. At this time, the telescopic mechanism 4 is in the retracted state, so that the stirring mechanism 3 is separated from the inner wall of extraction tank 1, and the material solution is stirred and mixed by the stirring mechanism 3.

[0061] Under this condition, the stirring blade 303 maintains an inclined posture of 15° to 60° under the elastic torque of the torsion spring 10. This not only avoids the high viscosity of the small intestinal mucosal erosion from causing a violent impact on the blade and effectively reduces the stirring resistance, but also ensures that the enzyme solution and the mucosal erosion are mixed radially and uniformly.

[0062] Step 3: Keep the mixture warm and stir until the protein in the solution is fully hydrolyzed and the heparin is completely dissociated and released, then terminate the enzymatic hydrolysis reaction.

[0063] Step 4: After draining the liquid from the extraction tank 1, control the linkage shaft 11 to move downwards, drive the telescopic mechanism 4 to extend, so that the stirring mechanism 3 fits against the inner wall of the extraction tank 1, and scrapes and cleans the inner wall of the extraction tank 1 through the stirring mechanism 3.

[0064] During this process, the gear 6 meshes with the rack 8 to drive the rotating shaft 302 to rotate, and the stirring blade 303 gradually switches from an inclined posture to a horizontal posture that is radially parallel to the tank wall. The blade edge is in close contact with the tank wall, and at the same time, the auxiliary scraper 13 at the lower end of the linkage shaft 11 moves down synchronously and contacts the bottom wall of the extraction tank 1.

[0065] Restart the rotary drive mechanism 5, and the stirring blades 303 rotate with the stirring shaft 2 to continuously scrape the tank wall in the full circumference. The auxiliary scraper 13 simultaneously scrapes and cleans the residual film residue at the bottom of the tank, achieving synchronous cleaning of the tank wall and the bottom.

[0066] After cleaning, the drive linkage shaft 11 moves upward and resets, the telescopic mechanism 4 retracts accordingly, the stirring mechanism 3 and the auxiliary scraper 13 return to their initial separated state, and the equipment is ready for the next batch of production.

[0067] Using the above method, low-resistance and high-efficiency mixing is achieved in the enzymatic hydrolysis stage, ensuring heparin activity and enzymatic hydrolysis efficiency; in the cleaning stage, simultaneous cleaning of the tank wall and tank bottom is achieved without the need for additional external tools, significantly improving the efficiency of continuous industrial production.

[0068] It should be noted that, in this document, relational terms such as "first" and "second" are used merely to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such a process, method, article, or apparatus. Without further limitations, an element defined by the phrase "comprising one..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes the element.

[0069] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A heparin sodium extraction device, comprising an extraction tank, characterized in that, Also includes: The stirring shaft is arranged along the central axis of the extraction tank and can rotate around the central axis of the extraction tank; Two stirring mechanisms are symmetrically distributed on both sides of the stirring shaft; the extension direction of each stirring mechanism is parallel to the axial direction of the stirring shaft. Two telescopic mechanisms correspond one-to-one with two stirring mechanisms; one end of each telescopic mechanism is fixedly connected to the stirring shaft, and the other end is fixedly connected to the corresponding stirring mechanism, which is used to drive the stirring mechanism to extend and retract radially along the extraction tank so that the stirring mechanism is in contact with or separates from the inner wall of the extraction tank.

2. The heparin sodium extraction equipment according to claim 1, characterized in that: A rotary drive mechanism is fixedly installed on the top of the extraction tank. The output end of the rotary drive mechanism is connected to the stirring shaft for driving the stirring shaft to rotate around the central axis of the extraction tank.

3. The heparin sodium extraction equipment according to claim 1, characterized in that: The stirring mechanism includes a C-shaped frame fixedly connected to one end of the telescopic mechanism, and the opening of the C-shaped frame is set away from the stirring shaft; A rotating shaft is rotatably connected within a C-shaped frame, and the rotating shaft is capable of rotating around its own axis; a stirring blade is fixedly sleeved on the rotating shaft, and the length direction of the stirring blade is parallel to the axial direction of the stirring shaft; When the stirring blades are in contact with the inner wall of the extraction tank, the width direction of the stirring blades is parallel to the radial direction of the extraction tank. When the stirring blades separate from the inner wall of the extraction tank, the width direction of the stirring blades forms a preset angle with the radial direction of the extraction tank.

4. The heparin sodium extraction equipment according to claim 3, characterized in that: The angle between the width of the stirring blade and the radial direction of the extraction tank is preset to be 15° to 60°.

5. The heparin sodium extraction equipment according to claim 3, characterized in that: Gears are fixedly sleeved at both the upper and lower ends of the rotating shaft. Connecting brackets corresponding to each gear are fixedly connected to the side wall of the stirring shaft. A rack adapted to the corresponding gear is fixedly connected to each connecting bracket. The extension direction of the rack is the same as the extension direction of the C-shaped frame. When the C-shaped frame extends or retracts radially along the extraction tank, the rotating shaft is driven to rotate around its own axis through the cooperation of the rack and pinion.

6. The heparin sodium extraction equipment according to claim 5, characterized in that: Both ends of the C-shaped frame are fixedly connected to housings, and the end of the rotating shaft is movably inserted into the corresponding housing. Each housing is equipped with a torsion spring, one torsion arm of which is connected to the inner wall of the housing, and the other torsion arm is connected to the shaft of the rotating shaft. When the gear and rack disengage, the elastic torque of the torsion spring drives the rotating shaft to rotate around its own axis, so that the width direction of the stirring blades forms a preset angle with the radial direction of the extraction tank.

7. The heparin sodium extraction equipment according to claim 1, characterized in that: The telescopic mechanism includes several sleeves that are fixed parallel to and spaced apart on the side wall of the stirring shaft, and each sleeve extends radially along the extraction tank. A slider is slidably connected inside each sleeve, and the slider can move along the axial direction of the sleeve; a connecting rod is fixedly connected between the slider and the corresponding stirring mechanism, and the connecting rod moves through the end wall of the corresponding sleeve.

8. The heparin sodium extraction equipment according to claim 7, characterized in that: The stirring shaft is a hollow shaft, and a linkage shaft is movably inserted inside the stirring shaft. The linkage shaft can move along the axial direction of the stirring shaft. A linkage rod corresponding to each slider is hinged on the linkage shaft, and the end of each linkage rod away from the linkage shaft is hinged to the corresponding slider. When the linkage shaft moves along the axial direction of the stirring shaft, it drives the slider to move along the axial direction of the sleeve via the linkage rod.

9. The heparin sodium extraction equipment according to claim 8, characterized in that: The lower end of the linkage shaft extends to the outside of the stirring shaft, and an auxiliary scraper is fixedly installed at the lower end of the linkage shaft. The outline of the auxiliary scraper is adapted to the inner bottom wall of the extraction tank. When the stirring mechanism separates from the inner wall of the extraction tank, the auxiliary scraper separates from the inner bottom wall of the extraction tank. When the stirring mechanism is in contact with the inner wall of the extraction tank, the auxiliary scraper is in contact with the inner bottom wall of the extraction tank.

10. An extraction method applicable to the heparin sodium extraction equipment according to any one of claims 1-9, characterized in that, Includes the following steps: Step 1: Put the small intestinal mucosal erosion into the extraction tank, adjust the pH of the solution to the range suitable for enzymatic hydrolysis, and heat to the enzymatic hydrolysis temperature; Step 2: Add protease solution into the extraction tank and simultaneously drive the stirring shaft to rotate; at this time, the telescopic mechanism is in the retracted state to separate the stirring mechanism from the inner wall of the extraction tank, and the material solution is stirred and mixed by the stirring mechanism; Step 3: Keep the mixture warm and stir until the protein in the solution is fully hydrolyzed and the heparin is completely dissociated and released; Step 4: After draining the liquid from the extraction tank, extend the telescopic mechanism to make the stirring mechanism fit against the inner wall of the extraction tank, and use the stirring mechanism to scrape and clean the inner wall of the extraction tank.

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