A low-temperature aerobic denitrifying Pseudomonas silesiensis W2-12b and its application

By screening and applying the low-temperature aerobic denitrifying strain Pseudomonas silesiensis W2-12b from Antarctic environmental samples, the problem of low nitrogen removal efficiency under low-temperature conditions was solved, achieving efficient wastewater treatment, especially in cold regions.

CN122104528APending Publication Date: 2026-05-29POLAR RES INST OF CHINA

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
POLAR RES INST OF CHINA
Filing Date
2026-04-09
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing aerobic denitrifying bacteria exhibit reduced denitrification efficiency and easy accumulation of nitrite under low-temperature conditions, limiting the effectiveness of wastewater treatment in cold regions.

Method used

A low-temperature, high-efficiency aerobic denitrifying strain, Pseudomonas silesiensis W2-12b, derived from Antarctic environmental samples, was screened. Through whole-genome sequencing and functional annotation, it was found that it possesses a complete denitrification pathway and multiple low-temperature adaptation-related genes, making it suitable for wastewater treatment.

Benefits of technology

At 15℃, Pseudomonas silesiensis W2-12b exhibited highly efficient denitrification capabilities, with a nitrate removal rate exceeding 99.8% and a nitrite accumulation rate below 1.5 μmol/L, providing a novel functional bacterial strain resource for wastewater treatment under low-temperature conditions.

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Abstract

The application relates to a low-temperature aerobic denitrifying Pseudomonas ciliicola Pseudomonas silesiensis W2-12b and application thereof belong to the field of microbial technology. The strain expands the resource library of aerobic denitrifying bacteria in a polar low-temperature environment, and provides theoretical support for revealing the aerobic denitrifying mechanism under cold conditions and optimizing the sewage treatment process in cold regions.
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