GhPDF1 gene for regulating and controlling included angle of fruit branches of upland cotton and application of GhPDF1 gene

By identifying the GhPDF1 gene in upland cotton and utilizing genetic engineering and molecular marker technology, the problem of low efficiency in cotton plant type improvement in existing technologies has been solved. This has enabled early, rapid, and accurate screening of compact plant types and efficient breeding, filling the research gap in genes regulating fruit branch angle.

CN122104725APending Publication Date: 2026-05-29GANSU AGRI UNIV
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Patent Information

Application Number
CN202511781303.6
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-11-29
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing technologies lack key gene targets and molecular markers for the fruiting branch angle of upland cotton, resulting in cotton plant type improvement relying on traditional field phenotypic identification, which is inefficient and easily affected by environmental interference, making it difficult to achieve early, rapid, and accurate screening of compact plant types.

Method used

The GhPDF1 gene was identified and confirmed to be a key gene regulating the fruit branch angle of upland cotton. GhPDF1 expression was suppressed by genetic engineering or molecular marker-assisted breeding was used to screen for compact plant types, including the construction of VIGS silencing recombinant vectors and Agrobacterium-mediated gene silencing, combined with PCR amplification and sequencing screening of G/C single nucleotide polymorphism sites.

Benefits of technology

It provides a precise genetic improvement pathway, significantly increasing the fruit branch angle by inhibiting GhPDF1 gene expression, thus achieving the cultivation of a compact cotton plant type, improving breeding efficiency, shortening the breeding cycle, and providing an efficient molecular marker-assisted breeding tool to ensure early, rapid, and accurate screening of compact plant types.

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Abstract

The invention relates to the technical field of biology, and discloses a GhPDF1 gene for regulating and controlling an included angle between fruit branches of upland cotton and application of the GhPDF1 gene, and the sequence of a coding region of the GhPDF1 gene is as shown in SEQ ID NO: 1; the gene ID (Identity) of the GhPDF1 gene is GHD04G0386.1; the application of the GhPDF1 gene in regulating and controlling the fruit branch included angle of the upland cotton is applied to regulating and controlling the fruit branch included angle of the upland cotton or cultivating a compact plant type upland cotton variety, and the GhPDF1 gene regulates and controls the fruit branch included angle of the upland cotton by regulating the expression level of the GhPDF1 gene in upland cotton cells. An accurate genetic improvement approach is provided, it is proved that by inhibiting expression of the GhPDF1 gene through the VIGS technology, the included angle between the fruit branches of cotton can be remarkably increased, the plant type becomes loose, and a definite gene target and an effective technical means are provided for cultivating new varieties of compact plant types of cotton through the genetic engineering technology.
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