Microorganism engineering strain of thraustochytrid family with low dpa content and construction and application thereof

By knocking out the OrfB subunit allele of PUFA synthase in microorganisms of the Chytridactycetes family, an engineered strain was constructed, solving the problem of the difficulty in reducing DPA content in existing technologies, and realizing the production of high-purity DHA oil, which has broad application prospects.

CN122104764APending Publication Date: 2026-05-29QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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Patent Information

Application Number
CN202610215598.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-02-13
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing technologies struggle to significantly reduce DPA content in the lipids of Cytomycetes without affecting DHA production, and the introduction of exogenous genes may impact strain safety.

Method used

By knocking out or inactivating any allele of the OrfB subunit of PUFA synthase in microorganisms of the Chrystrophariaceae family, engineered strains are constructed using methods such as homologous recombination and site-directed mutagenesis to reduce the DPA content in oils.

Benefits of technology

It significantly reduced the DPA content in oils while maintaining or increasing DHA production, avoiding the safety risks of introducing exogenous genes and improving the purity and modification potential of DHA oils.

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Abstract

The application provides a low-DPA-content thraustochytriales microbial engineering strain and a construction method and application thereof. The thraustochytriales microorganism is specifically a schizochytrium, aureococcus, urkella, thraustochytrium or hondaea. The thraustochytriales microbial engineering strain is obtained by knocking out one allele of a B subunit (OrfB) in a PUFA synthetase, and is a low-DPA-content engineering strain. The fermentation of the engineering strain for producing DHA-containing oil can significantly reduce the DPA content in the oil without affecting the DHA yield; the technical effect is realized by using a gene knockout method, and no exogenous gene is introduced, thereby reducing the possible safety problems in the food field, overcoming the problems in the prior art, and achieving a remarkable technical effect. In addition, the engineering strain improves the upper limit of the DHA content that can be achieved by schizochytrium modification by reducing the DPA content in the total fat, thereby laying an important foundation for subsequent research and development.
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Citation Information

Patent Citations

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  • Schizochytrium limacinum genetic engineering strain capable of reducing DPA proportion in grease and correspondingly increasing DHA content, method and application of schizochytrium limacinum genetic engineering strain

    CN120574686A