Use of UGT5 gene and SCPL12 gene in production of agerretine
CN122104770APending Publication Date: 2026-05-29SHANGHAI CHENSHAN BOTANICAL GARDEN
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SHANGHAI CHENSHAN BOTANICAL GARDEN
- Filing Date
- 2024-11-28
- Publication Date
- 2026-05-29
AI Technical Summary
Technical Problem
Current technology has not effectively utilized the rooting system of Leonurus japonicus to produce leonurine, resulting in insufficient yield.
Method used
Recombinant vectors of the UGT5 and SCPL12 genes were constructed using genetic engineering techniques, and these genes were overexpressed in transgenic plants using Agrobacterium rhizogenes-mediated technology to increase the synthesis of leonurine.
Benefits of technology
Experimental results show that the content of leonurine in the roots of transgenic plants that simultaneously overexpress UGT5 and SCPL12 is significantly increased, providing a basis for industrial production.
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Figure CN122104770A_ABST
Abstract
The application provides application of UGT5 genes and SCPL12 genes in production of agrostemma herb base in hairy roots. The application is realized by designing primer compositions to amplify the UGT5 genes and the SCPL12 genes, constructing a recombination carrier by using a Gateway technology, transforming the recombination carrier into Agrobacterium rhizogenes to obtain an Agrobacterium rhizogenes strain containing the UGT5 and the SCPL12, infecting leaves of Leonurus japonicus by using the Agrobacterium rhizogenes strain, culturing to obtain transgenic Leonurus japonicus hairy roots, and further producing the agrostemma herb base in the hairy roots. The content of the agrostemma herb base in the hairy roots of Leonurus japonicus obtained by using the Agrobacterium rhizogenes mediation and simultaneously overexpressing the UGT5 and the SCPL12 is higher than that of a control group. The method lays a foundation for production of the agrostemma herb base in transgenic plant cells.
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