A kit for pre-treatment of a dihydroxy vitamin d metabolite and a method for pre-treating a sample

Abstract

The application provides a double-hydroxy vitamin D metabolite pretreatment kit and a sample pretreatment method, and belongs to the technical field of in vitro diagnostic reagents. The double-hydroxy vitamin D metabolite pretreatment kit provided by the application comprises a magnetic bead working solution, the magnetic bead working solution comprises hydrophilic-lipophilic balance magnetic beads with a surface modified benzofuran ring system; a sample processing solution, the sample processing solution is a zinc sulfate heptahydrate solution; a leaching solution, the leaching solution is a solution containing fatty acid methyl ester ethoxylate and acetic acid; an eluent, the eluent is an acetonitrile-isopropyl alcohol mixed solution containing 2,6-di-tert-butyl-p-cresol; and a sample diluent, the sample diluent is a solution containing acetic acid. The kit provided by the application has the advantages of good stability, low detection limit and simultaneous detection of multiple target objects.

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