A highly efficient transdermal absorption serum based on plant extracts and its preparation method

Abstract

This invention belongs to the field of cosmetic technology and discloses a highly efficient transdermal absorption essence based on plant extracts and its preparation method. The essence uses purified water as the main matrix, combined with bearberry leaf extract (after removing lipid-soluble impurities) and chestnut extract (after removing tannins and pigments), along with panthenol and polyhexamethylene biguanide hydrochloride. The components exhibit good synergistic effects. The preparation method involves first layering the powders of the two plant extracts, wetting them with panthenol along the inner wall of a container, then adding purified water and stirring until homogeneous. After adding an antibacterial agent and stirring thoroughly, the mixture is filtered to obtain the final product. This invention, through a unique powder arrangement and mixing method, improves the dispersion uniformity and system stability of the plant active ingredients, enhances transdermal absorption efficiency, and results in a gentle and safe product that combines natural skincare properties and efficacy, making it suitable for large-scale production and practical application.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a highly efficient transdermal absorption serum based on plant extracts and its preparation method. Background Technology

[0002] Plant extracts, due to their gentleness, safety, and multiple skincare benefits, have become an important component of cosmetic ingredients. Among them, bearberry leaf extract and chestnut extract are widely used in serums and other skincare products due to their value in brightening, soothing, and repairing the skin. However, the stratum corneum, as the body's natural barrier, hinders the penetration of most hydrophilic active ingredients into the epidermis. This results in low bioavailability of plant active ingredients such as bearberry leaf extract and chestnut extract, making it difficult for them to effectively reach their target sites, thus limiting the efficacy of these products.

[0003] Currently, many common methods used in the industry to enhance transdermal absorption of ingredients have significant limitations. Delivery technologies such as nanocarriers and liposomes are complex and costly to produce, making them difficult to adapt to the industrial production of mass-market skincare products. While some synthetic penetration enhancers can improve penetration, they can easily irritate the skin and reduce product safety. Furthermore, compound systems based on natural plant extracts are mostly prepared using simple physical mixing methods, resulting in uneven ingredient dispersion and insufficient system stability, failing to achieve a balance between gentleness and high transdermal absorption.

[0004] Compared to single plant extracts, compound plant essences offer greater synergistic efficacy. However, current technologies struggle to address the challenge of simultaneously and efficiently achieving transdermal absorption of multiple plant active ingredients within a compound system. Furthermore, there is a lack of gentle penetration-enhancing solutions suitable for compound systems containing bearberry leaf extract and chestnut extract. The market demand for natural, safe, non-irritating, and highly transdermal plant-based essences continues to rise. Therefore, developing an essence product that effectively addresses these shortcomings has become a pressing technical challenge in this field. Summary of the Invention

[0005] In order to overcome the above-mentioned defects of the prior art, the embodiments of the present invention provide a highly efficient transdermal absorption essence based on plant extracts and its preparation method, which solves the problems of low transdermal efficiency of active ingredients in compound plant essences, poor system dispersion stability, and inability to balance mildness and efficient transdermal absorption in the prior art.

[0006] To address the above problems, the present invention provides the following technical solution: A highly effective transdermal absorption essence based on plant extracts comprises, by weight, the following components: 80-95 parts purified water, 0.5-3 parts bearberry leaf extract, 0.3-2.4 parts chestnut extract, 0.2-2 parts panthenol, and 0.1-0.3 parts polyhexamethylene biguanide hydrochloride; wherein the bearberry leaf extract is an aqueous extract after the removal of fat-soluble impurities, and the chestnut extract is an alcoholic aqueous extract after the removal of tannins and pigments.

[0007] Furthermore, the weight ratio of the bearberry leaf extract to the chestnut extract is 1:0.6-1:0.8. This weight ratio range is set based on the water solubility and powder dispersibility of the two plant extracts, which enables them to achieve compatibility balance in an aqueous system and avoids compatibility instability problems such as powder agglomeration and system stratification due to imbalance in the ratio.

[0008] Further, the preparation process of the bearberry leaf extract is as follows: bearberry leaf raw material is pulverized, purified water is added at a material-to-liquid ratio of 1:8-12, and reflux extraction is performed at 80-90℃ for 2-3 hours. The extract is then filtered to obtain the extract. 10-15% (v / v) of n-butanol is added to the extract, and after standing and separation, the lower aqueous phase is collected. This extraction is repeated 2-4 times. The final aqueous phase is concentrated under reduced pressure at 30-35℃ and -0.06~-0.08MPa until the n-butanol residue is ≤100mg / kg. The extract is then dried under vacuum at 35-40℃ and -0.07~-0.09MPa to obtain the bearberry leaf extract. Water reflux extraction enriches hydrophilic active ingredients, n-butanol extraction removes lipid-soluble impurities, and reduced pressure concentration and vacuum drying control solvent residue, thereby improving the water solubility and purity of the extract and reducing the adverse effects of impurities on formulation stability and skin compatibility.

[0009] Further, the preparation process of the *Citrus aurantium* extract is as follows: *Citrus aurantium* raw material is pulverized, and 50-70% (v / v) of ethanol aqueous solution is added at a material-to-liquid ratio of 1:10-15. The mixture is ultrasonically extracted at 60-70℃ for 1-2 hours, and filtered to obtain the extract. The extract is concentrated under reduced pressure at 40-45℃ and -0.08~-0.1MPa until the residual ethanol content is ≤100mg / kg. It is then redissolved with 30-40% (v / v) of ethanol aqueous solution. The pH of the redissolved solution is adjusted to 5.0-6.0 using a food-grade citrate-disodium hydrogen phosphate buffer system. 0.5-1% (v / v) of pharmaceutical-grade activated carbon is added, and the mixture is stirred at 25-30℃ for 10-15 minutes and then filtered. The filtrate is concentrated under the same reduced pressure until the residual ethanol content is ≤100mg / kg, and then dried under vacuum at 35-40℃ and -0.07~-0.09MPa to obtain the *Citrus aurantium* extract. The yield of active ingredients is improved by ultrasonic extraction with ethanol aqueous solution. pH adjustment and activated carbon adsorption remove tannins and pigment impurities, reduce the turbidity and irritation of the extract, and improve its compatibility with panthenol and polyhexamethylene biguanide hydrochloride.

[0010] This invention uses purified water as a matrix and selects bearberry leaf water extract (after removing fat-soluble impurities) and shishili alcohol water extract (after removing tannins and pigments) as core plant components. Panthenol is added to improve the compatibility between components, and polyhexamethylene biguanide hydrochloride is added to maintain the antibacterial stability of the system. Through the compounding of natural plant extracts and suitable excipients, a stable and skin-friendly water-based essence system is formed.

[0011] As a general inventive concept, this invention provides a method for preparing a highly efficient transdermal absorption serum based on plant extracts, as described above, comprising the following steps: S1. Weigh each raw material according to its weight parts; S2. Place the bearberry leaf extract powder in the lower layer of the container, and evenly spread the chestnut extract powder on the upper part of the bearberry leaf extract powder to form a layered powder arrangement structure. S3. Under constant temperature conditions of 25-28℃, panthenol is guided along the inner wall of the container at a rate of 1-2 mL / min, so that panthenol vertically passes through the powder layered structure from top to bottom for wetting treatment. The wetting treatment time is 5-8 min. S4. Add all the purified water to the container and stir at 220-280 r / min for 12-18 min until the system is homogeneous; S5. Add polyhexamethylene biguanide hydrochloride to the system and stir at 320-380 r / min for 6-10 min; S6. The system is filtered using a microporous membrane with a diameter of 0.22-0.45 μm until the system is clear, thus obtaining the essence.

[0012] Furthermore, in step S2, the height of the bearberry leaf extract powder layer is 2-3 cm, and the height of the chestnut extract powder layer is 1-2 cm. The chestnut extract powder is evenly spread on top of the bearberry leaf extract powder using a flat spreading and scraping method. Limiting the powder layer height and using a flat spreading and scraping method creates a regular layered structure, eliminates uneven powder accumulation, ensures uniform penetration of panthenol during wetting, and avoids insufficient wetting in certain areas.

[0013] Furthermore, during the wetting process in step S3, the container remains stationary and does not shake. Keeping the container stationary during the wetting process maintains the undisturbed layered structure of the powder, ensuring that panthenol penetrates the layers vertically along a fixed path, resulting in consistent wetting of the two extract powders and providing a stable foundation for subsequent mixing.

[0014] Furthermore, in step S4, the stirring is carried out at a constant speed in the same direction, without any speed change during the stirring process. Using a constant speed in the same direction maintains a constant shear force in the system, preventing sudden changes in shear force from causing the wetted powder to re-agglomerate, ensuring that the extract powder and the aqueous phase are quickly and thoroughly mixed, and maintaining the stable dispersion of the system.

[0015] Furthermore, after stirring in step S5, the system is allowed to stand for 3-5 minutes before proceeding to step S6 for filtration. This brief standing period after stirring allows tiny air bubbles to escape naturally, preventing them from clogging the filter membrane and reducing filtration efficiency. It also allows the components to come into full contact, improving the uniformity of the filtered essence.

[0016] Furthermore, in step S6, the filtration is performed using a pressurized air filtration method, with the filtration pressure controlled at 0.1-0.2 MPa. This quickly removes undispersed solid impurities, ensuring the clarity of the essence while avoiding high pressure that could disrupt the dispersion of the system.

[0017] The preparation method of the present invention targets the dispersion characteristics of two plant extract powders, and adopts a combination of layered arrangement and directional wetting to form a pre-dispersion system. The entire component is mixed by stirring with constant shear force, and the system is refined by gentle pressure filtration.

[0018] Compared with the prior art, the advantages of the present invention are: (1) The present invention adopts a pretreatment method of layered arrangement and directional wetting of plant extract powder, which can avoid the agglomeration problem caused by direct mixing of powder, establish a stable pre-dispersion state, provide a basis for subsequent mixing of all components, reduce the risk of phase separation of the system, and improve the process stability of the essence preparation process. (2) The present invention removes fat-soluble impurities from bearberry leaf extract and removes tannins and pigments from chestnut extract, which can reduce system compatibility conflicts caused by impurities, improve the compatibility of extracts with panthenol and antibacterial components, reduce turbidity and precipitation in finished products, and extend the storage stability of the system. (3) The mild pressure filtration process of the present invention can accurately remove undispersed solid particles in the system, ensuring the clarity of the finished product without destroying the stable dispersion structure that has been formed, maintaining the stability of the physicochemical state of the essence, and meeting the quality control requirements of cosmetic finished products. (4) The process of combining directional wetting and uniform stirring in this invention can reduce the intensity of high shear stirring, reduce the damage to the structure of plant active ingredients, retain the inherent characteristics of active ingredients, and improve the reliability and compatibility safety of the finished essence. (5) The present invention uses panthenol as a medium to complete directional wetting, which matches the surface characteristics of the two plant extracts, improves the powder wetting efficiency, shortens the mixing process time, simplifies the preparation process, and allows the system to quickly reach a stable state, which is suitable for industrial-scale production needs. Attached Figure Description

[0019] Figure 1 This is a comparison chart of the intensity reduction rate of keratinamide I band in various cases of this invention. Detailed Implementation

[0020] To facilitate understanding of the present invention, the present invention will be described more fully and in detail below with reference to the accompanying drawings and preferred embodiments, but the scope of protection of the present invention is not limited to the following specific embodiments.

[0021] Unless otherwise defined, all technical terms used herein have the same meaning as commonly understood by those skilled in the art. The technical terms used herein are for the purpose of describing particular embodiments only and are not intended to limit the scope of the invention.

[0022] Unless otherwise specified, all raw materials, reagents, instruments and equipment used in this invention can be purchased from the market or prepared by existing methods.

[0023] Example 1 A highly efficient transdermal absorption essence based on plant extracts of the present invention comprises, by weight, the following components: 95 parts purified water, 3 parts bearberry leaf extract, 2.4 parts chestnut extract, 2 parts panthenol, and 0.3 parts polyhexamethylene biguanide hydrochloride.

[0024] The bearberry leaf extract was prepared by crushing bearberry leaf raw material, adding purified water at a material-to-liquid ratio of 1:12, refluxing at 90℃ for 3 hours, filtering to obtain the extract, adding 15% n-butanol by volume to the extract, allowing it to stand and separate into layers, taking the lower aqueous phase, repeating the extraction 4 times, concentrating the final aqueous phase under reduced pressure at 35℃ and -0.08MPa until the n-butanol residue is ≤100mg / kg, and then vacuum drying at 40℃ and -0.09MPa.

[0025] The extract of Tianshi chestnut was prepared by crushing Tianshi chestnut raw materials and adding 70% (v / v) ethanol aqueous solution at a material-to-liquid ratio of 1:15. The mixture was ultrasonically extracted at 70℃ for 2 hours, filtered, and the extract was concentrated under reduced pressure at 45℃ and -0.1 MPa until the residual ethanol content was ≤100 mg / kg. The extract was then redissolved with 40% (v / v) ethanol aqueous solution. The pH of the redissolved solution was adjusted to 6.0 using a food-grade disodium citrate-phosphate buffer system. 1% (w / v) of pharmaceutical-grade activated carbon was added, and the mixture was stirred at 30℃ for 15 minutes and then filtered. The filtrate was concentrated under reduced pressure under the same conditions until the residual ethanol content was ≤100 mg / kg, and then vacuum dried at 40℃ and -0.09 MPa.

[0026] The preparation method of the highly efficient transdermal absorption serum based on plant extracts in this embodiment includes the following steps: S1. Weigh each raw material according to its weight parts; S2. Place the bearberry leaf extract powder in the lower layer of the container, with a layer height of 3cm. Spread the chestnut extract powder evenly on the upper part of the bearberry leaf extract powder using a flat spreading and scraping method, with a layer height of 2cm, forming a layered powder arrangement structure. The weight ratio of bearberry leaf extract to chestnut extract is 1:0.8. S3. Under constant temperature of 28℃, panthenol is guided along the inner wall of the container at a rate of 2mL / min, so that panthenol passes vertically through the powder layered structure from top to bottom for wetting treatment. The wetting treatment time is 8min. During the guiding and wetting process, the container is kept still and does not shake. S4. Add all the purified water to the container and stir at 280 r / min for 18 min until the system is homogeneous. Stirring should be done in the same direction at a constant speed, and there should be no speed change during the stirring process. S5. Add polyhexamethylene biguanide hydrochloride to the system and stir at 380 r / min for 10 min. After stirring, let the system stand for 5 min. S6. The system is subjected to air pressure filtration using a 0.45μm microporous membrane. The filtration pressure is controlled at 0.2MPa. The system is filtered until it is clear to obtain the essence.

[0027] Example 2 A highly efficient transdermal absorption essence based on plant extracts of the present invention comprises the following components by weight: 88 parts purified water, 2 parts bearberry leaf extract, 1.4 parts chestnut extract, 1 part panthenol, and 0.2 parts polyhexamethylene biguanide hydrochloride.

[0028] The bearberry leaf extract was prepared by crushing bearberry leaf raw material, adding purified water at a material-to-liquid ratio of 1:10, refluxing at 85℃ for 2.5 hours, filtering to obtain the extract, adding 12% (v / v) of n-butanol to the extract, allowing it to stand and separate into layers, taking the lower aqueous phase, repeating the extraction 3 times, concentrating the final aqueous phase under reduced pressure at 33℃ and -0.07MPa until the n-butanol residue is ≤100mg / kg, and then vacuum drying at 38℃ and -0.08MPa.

[0029] The extract of Tianshi chestnut was prepared by crushing Tianshi chestnut raw materials and adding 60% (v / v) ethanol aqueous solution at a material-to-liquid ratio of 1:12. The mixture was ultrasonically extracted at 65℃ for 1.5 h, filtered to obtain the extract, and concentrated under reduced pressure at 43℃ and -0.09 MPa until the residual ethanol content was ≤100 mg / kg. The extract was then redissolved with 35% (v / v) ethanol aqueous solution, and the pH of the redissolved solution was adjusted to 5.5 using a food-grade disodium citrate-phosphate buffer system. 0.8% (w / w) of pharmaceutical-grade activated carbon was added, and the mixture was stirred at 28℃ for 12 min and then filtered. The filtrate was concentrated under reduced pressure under the same conditions until the residual ethanol content was ≤100 mg / kg, and then vacuum dried at 38℃ and -0.08 MPa.

[0030] The preparation method of the highly efficient transdermal absorption serum based on plant extracts in this embodiment includes the following steps: S1. Weigh each raw material according to its weight parts; S2. Place the bearberry leaf extract powder in the lower layer of the container, with a layer height of 2.5cm. Spread the chestnut extract powder evenly on the upper part of the bearberry leaf extract powder using a flat spreading and scraping method, with a layer height of 1.5cm, forming a layered powder arrangement structure. The weight ratio of bearberry leaf extract to chestnut extract is 1:0.7. S3. Under constant temperature of 26℃, panthenol is guided along the inner wall of the container at a rate of 1.5mL / min, so that panthenol passes vertically through the powder layered structure from top to bottom for wetting treatment. The wetting treatment time is 6min. During the guiding and wetting process, the container is kept still and does not shake. S4. Add all the purified water to the container and stir at 250 r / min for 15 min until the system is homogeneous. Stirring should be done in the same direction at a constant speed, and there should be no speed change during the stirring process. S5. Add polyhexamethylene biguanide hydrochloride to the system and stir at 350 r / min for 8 min. After stirring, let the system stand for 4 min. S6. The system is subjected to air pressure filtration using a 0.3μm microporous membrane, with the filtration pressure controlled at 0.15MPa. The system is filtered until it becomes clear, and the essence is obtained.

[0031] Example 3 A highly efficient transdermal absorption essence based on plant extracts of the present invention comprises the following components by weight: 80 parts purified water, 0.5 parts bearberry leaf extract, 0.3 parts chestnut extract, 0.2 parts panthenol, and 0.1 parts polyhexamethylene biguanide hydrochloride.

[0032] The bearberry leaf extract was prepared by crushing bearberry leaves, adding purified water at a material-to-liquid ratio of 1:8, refluxing at 80°C for 2 hours, filtering to obtain the extract, adding 10% n-butanol (by volume) to the extract, allowing it to stand and separate into layers, taking the lower aqueous phase, repeating the extraction twice, and concentrating the final aqueous phase under reduced pressure at 30°C and -0.06 MPa until the n-butanol residue is ≤100 mg / kg, and then vacuum drying at 35°C and -0.07 MPa.

[0033] The extract of Tianshi chestnut was prepared by crushing Tianshi chestnut raw material and adding 50% (v / v) ethanol aqueous solution at a material-to-liquid ratio of 1:10. The mixture was ultrasonically extracted at 60℃ for 1 hour, filtered to obtain the extract, and concentrated under reduced pressure at 40℃ and -0.08 MPa until the residual ethanol content was ≤100 mg / kg. The extract was then redissolved with 30% (v / v) ethanol aqueous solution, and the pH of the redissolved solution was adjusted to 5.0 using a food-grade disodium citrate-phosphate buffer system. 0.5% (w / v) of pharmaceutical-grade activated carbon was added, and the mixture was stirred at 25℃ for 10 minutes and then filtered. The filtrate was concentrated under reduced pressure under the same conditions until the residual ethanol content was ≤100 mg / kg, and then vacuum dried at 35℃ and -0.07 MPa.

[0034] The preparation method of the highly efficient transdermal absorption serum based on plant extracts in this embodiment includes the following steps: S1. Weigh each raw material according to its weight parts; S2. Place the bearberry leaf extract powder in the lower layer of the container, with a layer height of 2cm. Spread the chestnut extract powder evenly on the upper part of the bearberry leaf extract powder using a flat spreading and scraping method, with a layer height of 1cm, forming a layered powder arrangement structure. The weight ratio of bearberry leaf extract to chestnut extract is 1:0.6. S3. Under constant temperature of 25℃, panthenol is guided along the inner wall of the container at a rate of 1mL / min, so that panthenol passes vertically through the powder layered structure from top to bottom for wetting treatment. The wetting treatment time is 5min. During the guiding and wetting process, the container is kept still and does not shake. S4. Add all the purified water to the container and stir at 220 r / min for 12 min until the system is homogeneous. Stirring should be done in the same direction at a constant speed, and there should be no speed change during the stirring process. S5. Add polyhexamethylene biguanide hydrochloride to the system and stir at 320 r / min for 6 min. After stirring, let the system stand for 3 min. S6. The system is subjected to air pressure filtration using a 0.22μm microporous membrane. The filtration pressure is controlled at 0.1MPa. The system is filtered until it is clear to obtain the essence.

[0035] Comparative Example The serum in this comparative example, by weight, comprises the following components: 90 parts purified water, 5 parts glycerin, 2 parts aloe vera extract, 2 parts oat extract, and 0.5 parts phenoxyethanol.

[0036] The preparation method of the serum in this comparative example includes the following steps: S1. Weigh each raw material according to the weight parts, add purified water to the mixing tank and heat to 45℃; S2. Add glycerol to purified water and stir at 300 r / min for 5 min until completely dissolved; S3. Add aloe vera extract and oat extract in sequence, and continue stirring for 10 minutes while maintaining the speed until the powder is evenly dispersed; S4. Cool to 30℃, add phenoxyethanol, and stir continuously for 8 minutes until the system is homogeneous; S5. After stirring, filter the solution under normal pressure using a 0.45μm microporous membrane until the system is clear, thus obtaining the essence.

[0037] The testing method is as follows: In vitro transdermal penetration depth: The Franz diffusion cell method was used to conduct in vitro experiments with the abdominal skin of Bama miniature pigs as a transdermal model. The maximum penetration depth of the active ingredients in the skin was determined by laser confocal Raman scanning microscopy. System clarity: The clarity of the essence system is directly determined by visually observing the appearance of the sample under natural light. Dispersion stability: The essence sample was placed under room temperature and sealed conditions for 30 days, and the dispersion stability of the system was evaluated by visually observing whether precipitation or stratification occurred. Keratinamide I band strength reduction rate: In vitro transdermal tests were performed using the Franz diffusion cell method. The intensity of keratinamide I bands was detected using laser confocal Raman scanning microscopy, and the strength reduction rate of the test group relative to the blank control group was calculated.

[0038] Table 1: Experimental Results of Examples 1-3 and Comparative Examples In summary, refer to Table 1 and Figure 1 In Examples 1-3 of this invention, the in vitro transdermal penetration depth ranged from 67-75 μm, while the comparative example showed a depth of only 35 μm. The layered powder arrangement and directional wetting process improved the uniformity of active ingredient dispersion, enhancing its penetration into the skin. The systems in the examples were clear and transparent, while the comparative example system was slightly turbid. Directional wetting combined with uniform stirring prevented powder agglomeration, whereas atmospheric pressure filtration was insufficient to remove small agglomerated particles. The examples showed no precipitation or layering after 30 days of standing at room temperature, while the comparative example showed slight precipitation and minor layering. Constant shear force stirring maintained the system's dispersion balance, while conventional mixing easily caused phase separation. The keratinamide I band strength reduction rate in the examples was 49.6%-54.3%, compared to only 20.5% in the comparative example. The uniformly dispersed active ingredients could directly act on keratin in the stratum corneum, altering its conformation to enhance skin permeability. This invention, through a combination of layered arrangement, directional wetting, and constant shear force stirring, significantly improves the transdermal penetration effect of the serum, system clarity, and long-term dispersion stability, effectively improving the stratum corneum permeability barrier.

Claims

1. A highly effective transdermal absorption serum based on plant extracts, characterized in that: The product comprises, by weight, 80-95 parts purified water, 0.5-3 parts bearberry leaf extract, 0.3-2.4 parts chestnut extract, 0.2-2 parts panthenol, and 0.1-0.3 parts polyhexamethylene biguanide hydrochloride; wherein the bearberry leaf extract is an aqueous extract after the removal of fat-soluble impurities, and the chestnut extract is an alcoholic aqueous extract after the removal of tannins and pigments.

2. The highly efficient transdermal absorption serum based on plant extracts according to claim 1, characterized in that: The weight ratio of bearberry leaf extract to chestnut extract is 1:0.6-1:0.

8.

3. The highly efficient transdermal absorption serum based on plant extracts according to claim 1, characterized in that: The preparation process of the bearberry leaf extract is as follows: bearberry leaf raw material is crushed, purified water is added at a material-to-liquid ratio of 1:8-12, and the mixture is refluxed at 80-90℃ for 2-3 hours. The extract is then filtered to obtain the extract. 10-15% (v / v) of n-butanol is added to the extract. After standing and separating into layers, the lower aqueous phase is taken and the extraction is repeated 2-4 times. The final aqueous phase is concentrated under reduced pressure at 30-35℃ and -0.06~-0.08MPa until the n-butanol residue is ≤100mg / kg. The extract is then dried under vacuum at 35-40℃ and -0.07~-0.09MPa to obtain the bearberry leaf extract.

4. The highly efficient transdermal absorption serum based on plant extracts according to claim 1, characterized in that: The preparation process of the Tianshi chestnut extract is as follows: crush the Tianshi chestnut raw material, add 50-70% ethanol aqueous solution at a material-to-liquid ratio of 1:10-15, and extract ultrasonically at 60-70℃ for 1-2 hours, then filter to obtain the extract. The extract was concentrated under reduced pressure at 40-45℃ and -0.08~-0.1MPa until the residual ethanol content was ≤100mg / kg. It was then redissolved in a 30-40% (v / v) aqueous ethanol solution. The pH of the redissolved solution was adjusted to 5.0-6.0 using a food-grade citrate-disodium hydrogen phosphate buffer system. 0.5-1% (w / w) of pharmaceutical-grade activated carbon was added, and the mixture was stirred at 25-30℃ for 10-15 min before filtration. The filtrate was concentrated under the same reduced pressure until the residual ethanol content was ≤100mg / kg. The extract was then dried under vacuum at 35-40℃ and -0.07~-0.09MPa to obtain the *Calendula officinalis* extract.

5. A method for preparing a highly efficient transdermal absorption serum based on plant extracts, characterized in that: Includes the following steps: S1. Weigh each raw material according to its weight parts; S2. Place the bearberry leaf extract powder in the lower layer of the container, and evenly spread the chestnut extract powder on the upper part of the bearberry leaf extract powder to form a layered powder arrangement structure. S3. Under constant temperature conditions of 25-28℃, panthenol is guided along the inner wall of the container at a rate of 1-2 mL / min, so that panthenol vertically passes through the powder layered structure from top to bottom for wetting treatment. The wetting treatment time is 5-8 min. S4. Add all the purified water to the container and stir at 220-280 r / min for 12-18 min until the system is homogeneous; S5. Add polyhexamethylene biguanide hydrochloride to the system and stir at 320-380 r / min for 6-10 min; S6. The system is filtered using a microporous membrane with a diameter of 0.22-0.45 μm until the system is clear, thus obtaining the essence.

6. The method for preparing a highly efficient transdermal absorption serum based on plant extracts according to claim 5, characterized in that: In step S2, the height of the bearberry leaf extract powder layer is 2-3cm, and the height of the chestnut extract powder layer is 1-2cm. The chestnut extract powder is evenly spread on the upper part of the bearberry leaf extract powder using a flat spreading and scraping method.

7. The method for preparing a highly efficient transdermal absorption serum based on plant extracts according to claim 5, characterized in that: During the guiding and wetting process in step S3, the container remains stationary and does not shake.

8. The method for preparing a highly efficient transdermal absorption serum based on plant extracts according to claim 5, characterized in that: In step S4, the stirring is carried out in the same direction at a constant speed, and there is no speed change during the stirring process.

9. The method for preparing a highly efficient transdermal absorption serum based on plant extracts according to claim 5, characterized in that: After stirring in step S5, let the system stand for 3-5 minutes before proceeding to step S6 for filtration.

10. The method for preparing a highly efficient transdermal absorption serum based on plant extracts according to claim 5, characterized in that: In step S6, the filtration is performed using a pneumatic pressurization method, with the filtration pressure controlled at 0.1-0.2 MPa.

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