Method for extracting chlorogenic acid substances from stevia rebaudiana waste residues and application of chlorogenic acid substances

Chlorogenic acids were extracted from stevia powder by alkaline extraction and column chromatography, and chlorogenic acid gel was prepared. This solved the problem of unutilized chlorogenic acids in stevia waste, realized the efficient utilization and functional application of resources, and improved the comprehensive utilization rate of stevia.

CN122124495APending Publication Date: 2026-06-02HEXI UNIV
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Patent Information

Application Number
CN202610325572.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-17
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

In existing technologies, the abundant chlorogenic acid substances in stevia waste have not been effectively utilized, leading to environmental pollution and resource waste. Moreover, chlorogenic acid is expensive, making it difficult to achieve its efficient extraction and comprehensive utilization.

Method used

A combination of alkaline extraction and column chromatography was used to extract chlorogenic acid from stevia powder. Chlorogenic acid gel was prepared by carboxylated graphene oxide and polyvinyl alcohol to achieve efficient loading and controlled release of chlorogenic acid.

Benefits of technology

This invention achieves a high-efficiency, green, and environmentally friendly extraction process for chlorogenic acid from stevia waste, improving the comprehensive utilization rate of stevia and solving the problems of environmental pollution and resource waste. At the same time, the chlorogenic acid gel has antioxidant and anti-inflammatory functions and can be widely used in the fields of food, medicine, and health products.

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Abstract

The invention discloses a method for extracting chlorogenic acid substances from stevia rebaudiana waste residues and application of the chlorogenic acid substances. The method comprises the following steps: extracting the chlorogenic acid substances from stevia rebaudiana powder by using an alkali extraction method; and determining whether the chlorogenic acid substances contain chlorogenic acid, isochlorogenic acid A and cryptochlorogenic acid or not. According to the method, stevia rebaudiana waste residues are used as raw materials, fat-soluble extract is obtained through solvent extraction, centrifugal filtration, reflux extraction and chromatographic separation, the fat-soluble extract is dissolved with ethyl alcohol and filtered, and a chlorogenic acid product is obtained through evaporation concentration and freeze drying. The chlorogenic acid substances in the stevia rebaudiana waste residues are extracted and developed, and the problems of environmental pollution and resource waste are solved. The extracted chlorogenic acid substance contains chlorogenic acid, cryptochlorogenic acid and isochlorogenic acid A, is low in sugar content, has the functions of oxidation resistance, inflammation resistance and blood pressure reduction, can be widely applied to the fields of food, medicines, health care products and the like, has high application value, and realizes comprehensive utilization of stevia rebaudiana.
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