A filter device for purifying Arenicola celluloase

Abstract

This invention relates to the field of plasmin separation and purification technology, and particularly to a filtration device for purifying sandworm plasmin. The device includes a filter cylinder, an inlet hopper at the top of the filter cylinder and an outlet hopper at the bottom of the filter cylinder, a cell fluid container rotatably mounted inside the filter cylinder, a drive assembly on the filter cylinder for rotating the cell fluid container, a suction assembly, an impurity collector, and a linkage drive mechanism on the filter cylinder. The linkage drive mechanism is connected to the filter element. The linkage drive mechanism, the cell fluid container, and the filter element cooperate to drive the suction assembly to move up and down for batch-switching suction filtration, and to drive the filter element to flip and pour impurities into the impurity collector. This invention ensures the efficiency and purity of cell fluid filtration through multiple cyclic filtrations and impurity collection. It solves the technical problems of existing technologies where repeated filtration is not possible and the collection and processing of impurities during filtration affects filtration efficiency and purity.

Description

Technical Field

[0001] This invention relates to the field of plasmin separation and purification technology, and in particular to a filtration device for purifying sandworm plasmin. Background Technology

[0002] The fibrinolytic enzyme contained in sandworms (marine organisms) has potential medicinal value in dissolving blood clots. Therefore, the extraction and purification of fibrinolytic enzyme from sandworm tissue is a research direction in the field of biopharmaceuticals. During the purification process, the broken sandworm cell fluid is usually filtered to remove solid impurities such as cell debris, obtaining a clear liquid containing the target protein.

[0003] Sandworm plasmin, also known as Sipunculus nudus plasmin, is extracted from the coelomic fluid and visceral tissue of fresh Sipunculus nudus. Currently, in the purification process of sandworm plasmin, the pretreatment and filtration of the solution after cell disruption are crucial steps to improve subsequent purification efficiency and ensure plasmin activity. Existing technologies typically employ a conventional route for sandworm plasmin purification: buffer homogenization – ammonium sulfate fractionation – chromatographic purification. The filtration step after cell disruption primarily removes cell debris and large molecular impurities, creating conditions for subsequent plasmin extraction and purification.

[0004] A search revealed Chinese patent CN 220846108 U, which discloses a separation and purification device for recombinant Bacillus subtilis fibrinolytic enzyme. The device includes a separation and purification machine and a separation component. The machine comprises a barrel and a feed hopper. The feed hopper is fixed to the feed inlet of the barrel. The separation component is arranged inside the barrel. The separation component includes a drive shaft, bearings, fixed plates, a fan wheel, a filter hopper, scrapers, and a scraping groove. The drive shaft is located inside the barrel. The inner ring of the bearing is fitted onto the drive shaft. Several fixed plates are arranged in a ring array and fixed to the outer ring of the bearing, with the ends of the fixed plates fixedly connected to the inner wall of the barrel. The fan wheel is fitted onto the drive shaft at the end away from the feed hopper. The filter hopper is fitted onto the drive shaft and fixed to the outer ring of the bearing at the side away from the fan wheel. Several scrapers are arranged in a ring array and fixed to the end of the drive shaft away from the fan wheel. Scraping grooves are formed on the scrapers. The scrapers contact the inner wall of the filter hopper.

[0005] The separation and purification device in the prior art automatically cleans the filter bucket with a scraper during the filtration process, but it cannot collect and process the impurities after the scraper cleaning. This causes the impurities to remain on the filter bucket during the filtration process, thus affecting the filtration efficiency. At the same time, the separation and purification device in the prior art cannot perform repeated filtration, thus failing to guarantee the purity of the filtered solution. Summary of the Invention

[0006] To address the technical problems in existing separation and purification devices, such as the inability to perform repeated filtration during the filtration process and the persistent retention of impurities on the filter bucket, which affects filtration efficiency and purity, this application provides a filtration device for purifying sandworm fibrinolytic enzyme. This device aims to ensure the efficiency and purity of cell fluid filtration through repeated cyclic filtration and the cyclical switching between filtration and impurity collection processes, thereby facilitating the extraction of sandworm fibrinolytic enzyme.

[0007] To achieve the above-mentioned technical objectives, the technical solution adopted by the present invention is as follows: This application provides a filtration device for purifying sandworm fibrinolytic enzyme, including a filter cylinder, an inlet hopper at the top of the filter cylinder, and an outlet hopper at the bottom of the filter cylinder. A cell fluid container is rotatably arranged inside the filter cylinder, and a baffle is slidably arranged inside the cell fluid container. A drive assembly is provided on the filter cylinder for driving the baffle to slide and the cell fluid container to rotate. The drive assembly is located above the baffle and cooperates with the cell fluid container. The filter cylinder is also provided with a liquid extraction assembly, an impurity collection component, and a linkage drive mechanism. The linkage drive mechanism is connected to the filter component. The linkage drive mechanism, the cell fluid container, and the filter component cooperate to drive the liquid extraction assembly to move up and down to perform batch switching liquid extraction filtration, and to drive the filter component to flip over and pour impurities into the impurity collection component. The impurity collection component is located below the filter component and engages with the linkage drive mechanism, and the liquid extraction assembly is located above the filter component.

[0008] Furthermore, the technical solution includes a sliding groove and an insertion groove on the cell fluid container frame. Multiple tension springs are connected inside the sliding groove, and the ends of the tension springs are connected to the baffles. The baffles slide in conjunction with the sliding groove.

[0009] Furthermore, the technical solution includes a motor at the bottom of the cell fluid container, with the output end of the motor connected to a screw. The screw is threaded into the cell fluid container, and a cover plate is connected to the end of the screw. The cover plate and the cell fluid container are respectively provided with mutually cooperating locking blocks.

[0010] In detail, the cell fluid receiving frame is a cylindrical structure with openings at the top and bottom. A cross-shaped partition divides the cylinder into four chambers. Each chamber has a wedge-shaped block at its top, and two sets of through slots are correspondingly formed on the cross-shaped partition, allowing each chamber to connect via these slots. Both sliding grooves and insertion slots are formed on the cross-shaped partition of the cell fluid receiving chamber, with the positions of the through slots corresponding to the positions of the sliding grooves. The insertion slots have a triangular structure.

[0011] More specifically, a limiting ring is fixedly fitted at the bottom of the cylinder, the limiting ring is rotatably engaged with a limiting groove opened on the filter cylinder, and an L-shaped locking block is provided at the bottom of the limiting ring.

[0012] Furthermore, the driving component includes a second motor fixedly connected to the inner wall of the filter cartridge, and an electric push rod connected to the output end of the second motor. The end of the electric push rod is engaged with the insertion slot.

[0013] Furthermore, the liquid extraction assembly includes a peristaltic pump fixedly installed inside the filter cartridge, with a liquid extraction tube installed inside the peristaltic pump. One end of the liquid extraction tube is located above the filter element, and the other end extends into the cell fluid container.

[0014] As an example, the filter element is a filter frame, and a filter screen is set inside the filter frame.

[0015] Further, the linkage drive mechanism includes a slide rod and a slider slidably connected to the filter cylinder. One end of the slide rod engages with the cell fluid container, and the other end engages with the slider. One end of the slider is connected to a spring A, the end of which is connected to the filter cylinder. The other end of the slider is connected to a pull wire A, the end of which is connected to a winding roller A. A synchronous belt roller A is connected to the winding roller A, and the synchronous belt roller A is rotatably connected to the filter cylinder. A synchronous belt roller B is also rotatably connected to the filter cylinder. A synchronous belt is provided on both the synchronous belt roller A and the synchronous belt roller B. A winding roller B is connected to the synchronous belt roller B, and a pull wire B is connected to the winding roller B. A counterweight is provided at the end of the pull wire B, and the pull wire B is connected to the suction pipe.

[0016] It should be noted that, in order to prevent the slide rod from rotating, a limit notch is made on the slide rod, and a limit protrusion that cooperates with the limit notch is provided on the filter cylinder.

[0017] Furthermore, the linkage drive mechanism also includes a pull wire C connected to the slider and a winding roller C rotatably connected to the filter cylinder. The pull wire C is connected to the winding roller C, and a transmission gear is connected to the end of the winding roller C. A transmission cylinder is connected to the transmission gear, and a filter element is connected to the transmission cylinder.

[0018] Furthermore, the impurity collecting component includes a collecting frame, a rack that meshes with a transmission gear on the collecting frame, the collecting frame being slidably connected to the filter cylinder, and a return spring connected to the collecting frame, the end of the return spring being connected to the filter cylinder.

[0019] Furthermore, the technical solution includes a cleaning assembly on the filter element. This assembly comprises a synchronous belt roller D rotatably mounted on the filter cylinder and a synchronous belt roller E rotatably connected to the peristaltic pump. A synchronous belt is mounted on both the synchronous belt roller D and the synchronous belt roller E. A motor is mounted on the filter cylinder, with its output end connected to the synchronous belt roller E. A winding roller D is connected to the synchronous belt roller D, and a pull wire D is connected to the winding roller D. The pull wire D passes over a pulley and connects to a sliding column. The sliding column is slidably connected to the filter cylinder and the transmission cylinder. One end of the sliding column is connected to a spring B, which is connected to the filter cylinder. The other end of the sliding column is connected to a toothed plate, which is slidably connected to the transmission cylinder.

[0020] The cleaning assembly also includes a gear rotatably mounted on the filter element, and a cleaning brush connected to the gear, which meshes with a toothed plate.

[0021] Compared with the prior art, the beneficial effects of the present invention are: This invention utilizes a coordinated drive mechanism, a cell fluid container, a filter element, and an impurity collector. As the cell fluid container rotates, it drives the suction tube to move from one filtration area to another, achieving a multi-cycle filtration effect and thus improving the purity of the cell fluid. During the switching of the suction tube areas, the drive mechanism rotates the filter element, and simultaneously, the impurity collector automatically slides out below the filter element to precisely catch the spilled impurities. After dumping, the impurity collector automatically resets under the action of a return spring. The entire process is highly automated, reducing manual operation.

[0022] This invention, through the design of the cleaning component, enables the peristaltic pump to drive the toothed plate to move back and forth during the peristaltic filtration process. In turn, the meshing of the toothed plate and gears drives the cleaning brush to rotate back and forth on the filter element, thereby cleaning the sticky impurities such as cell debris on the filter element. This helps the impurities fall smoothly into the collection frame, avoiding clogging of the filter element. This not only ensures the stability of filtration accuracy and efficiency, but also reduces the wear and tear on the filter element and extends its service life. Attached Figure Description

[0023] To more clearly illustrate the technical solutions of the embodiments of this application, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of this application and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained based on these drawings without creative effort.

[0024] Figure 1 This is a schematic diagram of the overall structure of the present invention; Figure 2 For the present invention Figure 1Transverse sectional perspective view; Figure 3 For the present invention Figure 2 Enlarged view of section A in the middle; Figure 4 For the present invention Figure 1 Left and right horizontal sectional views; Figure 5 For the present invention Figure 1 Front and rear transverse sectional views; Figure 6 For the present invention Figure 5 Enlarged view of section B; Figure 7 This is a cross-sectional view of the filter cartridge structure of the present invention; Figure 8 This is a three-dimensional view of the cell fluid containing frame structure of the present invention; Figure 9 This is a schematic diagram of the cell fluid containment frame structure of the present invention; Figure 10 For the present invention Figure 9 Sectional view along the AA direction; Figure 11 This is a perspective view of the partition structure of the present invention; Figure 12 This is a perspective view of the slide bar structure in this invention; Figure 13 This is a three-dimensional view of the drive component structure of the present invention; Figure 14 This is a diagram showing the connection relationship between the impurity collection element and the filter element of the present invention; Figure 15 For the present invention Figure 14 Mid-section view; Figure 16 For the present invention Figure 14 Vertical sectional view; Figure 17 This is a perspective view of the impurity collection component structure of the present invention; Figure 18 This is a three-dimensional view of the cleaning component structure of the present invention; Figure 19 This is a diagram showing the connection relationship between the gear and the cleaning brush in this invention; Figure 20 This is a three-dimensional view of the slider structure in this invention; In the diagram: 1. Filter cylinder; 2. Inlet hopper; 3. Outlet hopper; 4. Cell fluid container; 401. Slide groove; 402. Insertion groove; 403. Tension spring; 404. Motor 1; 405. Screw; 406. Cover plate; 407. Locking block; 5. Baffle plate; 6. Drive assembly; 601. Motor 2; 602. Electric push rod; 7. Liquid extraction assembly; 701. Peristaltic pump; 702. Liquid extraction tube; 8. Impurity collector; 801. Collection frame; 802. Rack; 803. Return spring; 9. Linkage drive mechanism; 901. Slide rod; 902. Slider; 903. Spring A; 904. Pull wire A; 905. Winding roller A; 906. Synchronous belt roller A; 907. Synchronous belt roller B; 908. Synchronous belt one; 909. Winding roller B; 9010. Pull wire B; 9011. Counterweight; 9012. Pull wire C; 9013. Winding roller C; 9014. Transmission gear; 9015. Transmission cylinder; 10. Filter element; 11. Cleaning assembly; 1101. Synchronous belt roller D; 1102. Synchronous belt roller E; 1103. Synchronous belt two; 1104. Motor three; 1105. Winding roller D; 1106. Pull wire D; 1107. Sliding column; 1108. Spring B; 1109. Toothed plate; 11010. Gear; 11011. Cleaning brush. Detailed Implementation

[0025] To enable those skilled in the art to better understand the technical solutions of the present invention, the technical solutions of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0026] In the description of this invention, it should be understood that the terms "upper", "lower", "front", "rear", "left", "right", "front end", "rear end", "upper end", "lower end", "left end", "right end", "inner side", "outer side", etc., indicate the orientation or positional relationship based on the orientation or positional relationship shown in the accompanying drawings. They are only for the convenience of describing this invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation. Therefore, they should not be construed as limitations on this invention.

[0027] In the description of this application, it should also be noted that, unless otherwise expressly specified and limited, the terms "set up," "install," "connect," and "link" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this application based on the specific circumstances.

[0028] Example 1 Reference Figure 1-20As shown, this embodiment provides a filtration device for purifying sandworm fibrinolytic enzyme, which aims to achieve the functions of automatically switching filtration zones to complete multiple filtrations and automatically removing impurities.

[0029] The main body of this device is a filter cylinder 1. The top of the filter cylinder 1 has an inlet hopper 2 for pouring in the broken sandworm cell solution to be filtered, and the bottom has an outlet hopper 3 for discharging the filtered clear liquid. At the center of the filter cylinder 1, a cell fluid receiving frame 4 is rotatably mounted for temporarily storing the cell fluid to be filtered. A baffle 5 is slidably mounted inside the cell fluid receiving frame 4, dividing the interior of the cell fluid receiving frame 4 into different chambers. A drive assembly 6 is mounted on the filter cylinder 1. This drive assembly 6 cooperates with the cell fluid receiving frame 4, driving both the baffle 5 to slide within the cell fluid receiving frame 4 and the entire cell fluid receiving frame 4 to rotate within the filter cylinder 1.

[0030] In addition, the filter cartridge 1 is also equipped with a liquid extraction assembly 7, an impurity collection component 8, a linkage drive mechanism 9, and a filter element 10. The liquid extraction assembly 7 is used to draw liquid from the cell fluid container 4 onto the filter element 10 for filtration. The linkage drive mechanism 9 is the core linkage part of the entire device, and it is connected to the filter element 10. When the cell fluid container 4 rotates, it triggers the linkage drive mechanism 9 to work, thereby realizing two main actions: first, driving the liquid extraction end of the liquid extraction assembly 7 to move up and down to switch the liquid extraction filtration area; second, driving the filter element 10 to flip, pouring the solid impurities trapped on its surface into the impurity collection component 8 below.

[0031] Among them, such as Figure 8-11 As shown, the cell fluid container 4 has a sliding groove 401 and an insertion groove 402. A baffle 5 is installed within the sliding groove 401 and connected to the inner wall of the sliding groove 401 via multiple tension springs 403, allowing the baffle 5 to maintain a balanced position when not subjected to external force and to have a tendency to return to its original position after being pushed by external force. A motor 404 is also installed at the bottom of the cell fluid container 4. The output end of the motor 404 is connected to a screw 405, which engages with a threaded hole at the bottom of the cell fluid container 4. A cover plate 406 is connected to the end of the screw 405, and locking blocks 407 are respectively provided on the edge of the bottom opening of the cover plate 406 and the cell fluid container 4. When the motor 404 drives the screw 405 to rotate, it can drive the cover plate 406 to move up and down. When the cover plate 406 rises and closes with the bottom of the cell fluid container 4, the locking block 407 engages with each other to seal the bottom of the cell fluid container 4 and prevent liquid leakage. When it is necessary to drain the residual liquid in the cell fluid container 4, the cover plate 406 descends and opens the bottom opening.

[0032] As an example, refer to Figure 8 , Figure 13As shown, the drive assembly 6 includes a second motor 601 fixedly connected to the inner wall of the filter cylinder 1. The output end of the second motor 601 is connected to an electric push rod 602, and the end of the electric push rod 602 is inserted into the insertion slot 402.

[0033] In actual use, after the cell fluid to be filtered is mixed evenly in the cell fluid container 4, the electric push rod 602 is activated to extend. The electric push rod 602 pushes the partition plate 5 down through the insertion groove 402, thereby separating the various areas of the cell fluid container 4.

[0034] In this embodiment, the liquid extraction assembly 7 includes a peristaltic pump 701 fixed inside the filter cartridge 1 and a liquid extraction tube 702 disposed inside the peristaltic pump 701. The outlet end of the liquid extraction tube 702 is suspended above the filter element 10, while the extraction end extends and inserts into a chamber of the cell fluid receiving frame 4. The peristaltic pump 701 generates negative pressure by squeezing the liquid extraction tube 702 with rollers, drawing the cell fluid from one chamber of the cell fluid receiving frame 4 and transporting it to the filter element 10 for filtration. The filtered cell fluid then enters another chamber of the cell fluid receiving frame 4 for the next cycle of filtration. This cycle repeats, enabling multiple filtrations of the cell fluid and thus improving its purity.

[0035] In this embodiment, the linkage drive mechanism 9 is key to achieving automated switching. It includes a slide bar 901 and a slider 902, both slidably connected to the filter cylinder 1. One end of the slide bar 901 extends into the rotation path of the cell fluid container 4. When the cell fluid container 4 rotates, the wedge-shaped protrusion at its upper end pushes the slide bar 901 upwards, causing the other end of the slide bar 901 to push the slider 902 upwards in coordination. One end of the slider 902 is connected to a spring A903, the other end of which is fixed to the filter cylinder 1, providing a restoring force for the slider 902. The other end of the slider 902 is connected to a pull wire A904, which is wound around a winding roller A905. The winding roller A905 is coaxially and fixedly connected to a synchronous belt roller A906. The synchronous belt roller A906 is driven by a synchronous belt 908 and a synchronous belt roller B907. The synchronous belt roller B907 is coaxially and fixedly connected to the winding roller B909. A tension wire B9010 is wound on the winding roller B909. A counterweight 9011 is connected to the end of the tension wire B9010, and the end of the tension wire B9010 is fixedly connected to the suction tube 702 (e.g., with a clamp). The counterweight 9011 is used to maintain the tension of the tension wire B9010 and assists the suction tube 702 in descending and resetting.

[0036] Preferably, in order to guide the pull wire B9010, a guide hole is provided on the filter cylinder 1, and the pull wire B9010 slides in the guide hole. Under the guidance of the guide hole, the end of the pull wire B9010 extends into the cell fluid container 4.

[0037] When the cell fluid container 4 rotates and pushes the slide bar 901, the slide bar 901 pushes the slider 902 to compress the spring A903. The slider 902 moves and pulls the pull wire A904, causing the winding roller A905 and the synchronous belt roller A906 to rotate. Through the transmission of the synchronous belt 908, the synchronous belt roller B907 and the winding roller B909 rotate synchronously, thereby winding up the pull wire B9010. When the pull wire B9010 is wound up, the suction tube 702 rises. Thus, as the cell fluid container 4 rotates by an angle (corresponding to a new chamber divided by the baffle 5), the suction end of the suction tube 702 moves from one chamber of the cell fluid container 4 to another. When the cell fluid container 4 stops rotating, under the gravity of the counterweight 9011, the pull wire B9010 is released, and the suction tube 702 descends to perform suction filtration in the new area.

[0038] Furthermore, the linkage drive mechanism 9 also includes a pull wire C9012 connected to the slider 902 and a winding roller C9013 rotatably connected to the filter cylinder 1. The pull wire C9012 is connected to the winding roller C9013, and a transmission gear 9014 is connected to the end of the winding roller C9013. A transmission cylinder 9015 is connected to the transmission gear 9014, and a filter element 10 is connected to the transmission cylinder 9015.

[0039] Furthermore, the impurity collection component 8 includes a collection frame 801, on which a rack 802 meshes with a transmission gear 9014. The collection frame 801 is slidably connected to the filter cylinder 1, and a return spring 803 is connected to the collection frame 801, with the end of the return spring 803 connected to the filter cylinder 1.

[0040] When slider 902 is pushed to its farthest end by slide bar 901, pull wire C9012 is pulled to its maximum extent, thereby driving winding roller C9013 and transmission gear 9014 to rotate at a large angle. The rotation of transmission gear 9014 will cause transmission cylinder 9015 and filter element 10 to flip together. At the same time, since transmission gear 9014 meshes with rack 802, the rotation of transmission gear 9014 will drive collection frame 801 to overcome the elastic force of return spring 803 and slide out from one side, which is exactly below the flipped filter element 10. After the filter element 10 has finished emptying the impurities, slider 902 returns to its original position under the action of spring A903, pull wire C9012 loosens, and transmission gear 9014 rotates in the opposite direction to return to its original position under the action of return spring 803 of collection frame 801. Collection frame 801 returns to its original position, and filter element 10 also flips back to the horizontal working position.

[0041] Example 2 Based on Example 1, in order to improve the purity of the cell fluid and prevent the filter element 10 from becoming clogged, a cleaning component 11 is provided on the filter element 10.

[0042] Among them, such as Figure 14-19 As shown, the cleaning assembly 11 includes a synchronous belt roller D1101 rotatably mounted on the filter cylinder 1 and a synchronous belt roller E1102 rotatably connected to the peristaltic pump 701, the two being connected by a synchronous belt 1103. A motor 1104 is mounted on the filter cylinder 1, and the output end of the motor 1104 is connected to the synchronous belt roller E1102. A winding roller D1105 is coaxially connected to the synchronous belt roller D1101, and a pull wire D1106 is wound on the winding roller D1105. The pull wire D1106 passes over a pulley fixed on the filter cylinder 1 and connects to a sliding column 1107. The sliding column 1107 is slidably connected to the filter cylinder 1 and the transmission cylinder 9015. A spring B1108 is connected to one end of the sliding column 1107, and the other end of the spring B1108 is connected to the filter cylinder 1, providing a restoring force for the sliding column 1107. The other end of the sliding column 1107 is fixedly connected to a toothed plate 1109, which is also slidably connected to the transmission cylinder 9015, allowing the toothed plate 1109 to move axially relative to the transmission cylinder 9015. A gear 11010 is rotatably mounted on the filter element 10, which is coaxially connected to the cleaning brush 11011 and meshes with the toothed plate 1109.

[0043] It should be noted that motor 3104 operates in alternating forward and reverse directions when starting.

[0044] During normal filtration, the peristaltic pump 701 operates while the motor 1104 is activated. The motor 1104 drives the synchronous belt roller E1102 to rotate, which in turn drives the synchronous belt roller D1101 and the winding roller D1105 to rotate via the synchronous belt 1103. This causes the wire D1106 to be wound up, pulling the sliding column 1107 and the toothed plate 1109 away from the winding roller D1105. At this time, the spring B1108 is stretched, and the position of the toothed plate 1109 relative to the filter element 10 changes, thus meshing with the gear 11010 and driving it to rotate. The gear 11010 drives the cleaning brush 11011 to rotate, cleaning the surface of the filter element 10 and removing adhering impurities. During the reciprocating rotation of the motor 1104, the synchronous belt roller E1102 rotates reciprocally. With the cooperation of the spring B1108, the toothed plate 1109 moves reciprocally, thereby achieving the reciprocating rotation and cleaning of the cleaning brush 11011.

[0045] Specific application process The following describes in detail the application of a filtration device for purifying sandworm fibrinolytic enzyme in the sandworm fibrinolytic enzyme filtration and extraction process, with reference to Examples 1 and 2.

[0046] In the specific use of the filtration device for purifying sandworm fibrinolytic enzyme, the motor 404 is started to rotate forward, which drives the screw 405 to rotate, causing the cover plate 406 to move upward. When the cover plate 406 rises and closes with the bottom of the cell fluid container 4, the locking block 407 engages with each other, so that the bottom of the cell fluid container 4 is sealed.

[0047] The sandworm cell fragments to be filtered are poured into the cell fluid container 4 through the inlet hopper 2 at the top of the filter cylinder 1. After the cell fluid to be filtered is mixed evenly in the cell fluid container 4, the electric push rod 602 is activated to extend. The electric push rod 602 pushes the baffle 5 down through the insertion groove 402, thereby separating the different areas of the cell fluid container 4.

[0048] The peristaltic pump 701 is activated. The pump generates negative pressure by squeezing the suction tube 702 through rollers, drawing the cell fluid from one chamber of the cell fluid container 4 and transferring it to the filter element 10 for filtration. The filtered cell fluid then enters another chamber of the cell fluid container 4 for further filtration. This cycle repeats, achieving multiple filtrations of the cell fluid and thus improving its purity.

[0049] While the peristaltic pump 701 is operating, motor 3 1104 is started. Motor 3 1104 drives the synchronous belt roller E1102 to rotate, which in turn drives the synchronous belt roller D1101 and the winding roller D1105 to rotate via the synchronous belt 2 1103. This causes the wire D1106 to be wound up, pulling the sliding column 1107 and the toothed plate 1109 away from the winding roller D1105. At this time, the spring B1108 is stretched, and the position of the toothed plate 1109 relative to the filter element 10 changes, thus meshing with the gear 11010 and driving it to rotate. The gear 11010 drives the cleaning brush 11011 to rotate, cleaning the surface of the filter element 10 and sweeping off the adhering impurities. During the reciprocating rotation of motor 3 1104, the synchronous belt roller E1102 reciprocates, and with the cooperation of spring B1108, the toothed plate 1109 moves back and forth, thereby realizing the reciprocating rotation of the cleaning brush 11011 for cleaning.

[0050] Once the cell fluid in the current chamber has been filtered, motor 601 is activated. Through the interaction of electric push rod 602 and insertion slot 402, the cell fluid container 4 rotates. This rotation pushes slide rod 901 upwards, causing slider 902 to compress spring A903. Sliding slider 902 pulls the cable A904, causing winding roller A905 and synchronous belt roller A906 to rotate. Through the transmission of synchronous belt 908, synchronous belt roller B907 and winding roller B909 rotate synchronously, thereby winding cable B9010. When the pull wire B9010 is wound up, the suction tube 702 rises. Thus, as the cell fluid container 4 rotates by an angle (corresponding to a new chamber divided by the baffle 5), the suction end of the suction tube 702 moves from one chamber of the cell fluid container 4 to the next. When the cell fluid container 4 stops rotating, the pull wire B9010 is released under the gravity of the counterweight 9011, and the suction tube 702 descends to perform suction filtration in the new area.

[0051] Synchronously, when slider 902 is pushed to its farthest end by slide bar 901, pull wire C9012 is pulled to its maximum extent, thereby driving winding roller C9013 and transmission gear 9014 to rotate at a large angle. The rotation of transmission gear 9014 will cause transmission cylinder 9015 and filter element 10 to flip together. At the same time, since transmission gear 9014 meshes with rack 802, the rotation of transmission gear 9014 will drive collection frame 801 to overcome the elastic force of return spring 803 and slide out from one side, just below the flipped filter element 10. After the filter element 10 has finished emptying the impurities, slider 902 returns to its original position under the action of spring A903, pull wire C9012 loosens, and transmission gear 9014 rotates in the opposite direction to return to its original position under the action of return spring 803 of collection frame 801. Collection frame 801 returns to its original position, and filter element 10 also flips back to the horizontal working position.

[0052] It should be noted that those skilled in the art can make various modifications and improvements without departing from the structure of this invention, and these should also be considered within the scope of protection of this invention. These modifications and improvements will not affect the effectiveness of the invention or the practicality of the patent. The scope of protection claimed in this application shall be determined by the content of its claims, and the specific embodiments described in the specification can be used to interpret the content of the claims.

Claims

1. A filtration device for purifying sandworm fibrinolytic enzyme, comprising a filter cylinder (1), an inlet hopper (2) disposed at the top of the filter cylinder (1), and an outlet hopper (3) disposed at the bottom of the filter cylinder (1), characterized in that: A cell fluid container (4) is rotatably arranged inside the filter tube (1), and a baffle (5) is slidably arranged inside the cell fluid container (4). A drive assembly (6) is provided on the filter tube (1) for driving the baffle (5) to slide and the cell fluid container (4) to rotate. The drive assembly (6) is located above the baffle (5) and cooperates with the cell fluid container (4). The filter cartridge (1) is also equipped with a liquid extraction component (7), an impurity collection component (8) and a linkage drive mechanism (9). The linkage drive mechanism (9) is connected to a filter component (10). The linkage drive mechanism (9), the cell fluid container (4) and the filter component (10) cooperate with each other to drive the liquid extraction component (7) to move up and down to perform batch switching liquid extraction and filtration, and to drive the filter component (10) to flip over and pour impurities into the impurity collection component (8). The impurity collection component (8) is located below the filter element (10) and engages with the linkage drive mechanism (9), while the liquid extraction component (7) is located above the filter element (10).

2. The filtration device for purifying sandworm fibrinolytic enzyme according to claim 1, characterized in that: The cell fluid container (4) is provided with a sliding groove (401) and a plug groove (402). Multiple tension springs (403) are connected in the sliding groove (401). The ends of the tension springs (403) are connected to the baffles (5). The baffles (5) and the sliding groove (401) slide together.

3. The filtration device for purifying sandworm fibrinolytic enzyme according to claim 2, characterized in that: A motor (404) is installed at the bottom of the cell fluid container (4). The output end of the motor (404) is connected to a screw (405). The screw (405) is threaded into the cell fluid container (4). A cover plate (406) is connected to the end of the screw (405). The cover plate (406) and the cell fluid container (4) are respectively provided with mutually cooperating locking blocks (407).

4. The filtration device for purifying sandworm fibrinolytic enzyme according to claim 1, characterized in that: The drive assembly (6) includes a second motor (601) fixedly connected to the inner wall of the filter cylinder (1). The output end of the second motor (601) is connected to an electric push rod (602), and the end of the electric push rod (602) is inserted into the insertion slot (402).

5. A filtration device for purifying sandworm fibrinolytic enzyme according to claim 1, characterized in that: The liquid extraction assembly (7) includes a peristaltic pump (701) fixedly installed in the filter cartridge (1), and a liquid extraction tube (702) is provided inside the peristaltic pump (701). One end of the liquid extraction tube (702) is located above the filter element (10), and the other end extends into the cell fluid container (4).

6. A filtration device for purifying sandworm fibrinolytic enzyme according to claim 5, characterized in that: The linkage drive mechanism (9) includes a slide rod (901) and a slider (902) that are slidably connected to the filter tube (1). One end of the slide rod (901) is engaged with the cell fluid container (4), and the other end is engaged with the slider (902). One end of the slider (902) is connected to a spring A (903), the end of the spring A (903) is connected to the filter cylinder (1), the other end of the slider (902) is connected to a pull wire A (904), the end of the pull wire A (904) is connected to a winding roller A (905), a synchronous belt roller A (906) is connected to the winding roller A (905), and the synchronous belt roller A (906) is rotatably connected to the filter cylinder (1); A synchronous belt roller B (907) is rotatably connected to the filter cylinder (1). A synchronous belt A (908) is provided on the synchronous belt roller A (906) and the synchronous belt roller B (907). A winding roller B (909) is connected to the synchronous belt roller B (907). A pull wire B (9010) is connected to the winding roller B (909). A counterweight (9011) is provided at the end of the pull wire B (9010). The pull wire B (9010) is connected to the liquid extraction pipe (702).

7. A filtration device for purifying sandworm fibrinolytic enzyme according to claim 6, characterized in that: The linkage drive mechanism (9) also includes a pull wire C (9012) connected to the slider (902) and a winding roller C (9013) rotatably connected to the filter cylinder (1). The pull wire C (9012) is connected to the winding roller C (9013). The end of the winding roller C (9013) is connected to a transmission gear (9014). The transmission gear (9014) is connected to a transmission cylinder (9015). The transmission cylinder (9015) is connected to a filter element (10).

8. A filtration device for purifying sandworm fibrinolytic enzyme according to claim 7, characterized in that: The impurity collection component (8) includes a collection frame (801), a rack (802) that meshes with a transmission gear (9014) is provided on the collection frame (801), the collection frame (801) is slidably connected to the filter cylinder (1), and a return spring (803) is connected on the collection frame (801), the end of the return spring (803) is connected to the filter cylinder (1).

9. A filtration device for purifying sandworm fibrinolytic enzyme according to claim 7, characterized in that: A cleaning assembly (11) is provided on the filter element (10). The cleaning assembly (11) includes a synchronous belt roller D (1101) rotatably mounted on the filter cylinder (1) and a synchronous belt roller E (1102) rotatably connected to the peristaltic pump (701). A synchronous belt second (1103) is provided on the synchronous belt roller D (1101) and the synchronous belt roller E (1102). A motor (1104) is installed on the filter cylinder (1). The output end of the motor (1104) is connected to the synchronous belt roller E (1102). A winding roller D (1105) is connected to the synchronous belt roller D (1101). A pull wire D (1106) is connected to the winding roller D (1105). The pull wire D (1106) passes around the pulley and is connected to the sliding column (1107). The sliding column (1107) is slidably connected to the filter cylinder (1) and the transmission cylinder (9015). One end of the sliding column (1107) is connected to a spring B (1108), which is connected to the filter cylinder (1). The other end of the sliding column (1107) is connected to a toothed plate (1109), which is slidably connected to the transmission cylinder (9015).

10. A filtration device for purifying sandworm fibrinolytic enzyme according to claim 9, characterized in that: The cleaning assembly (11) also includes a gear (11010) rotatably mounted on the filter (10) and a cleaning brush (11011) connected to the gear (11010), the gear (11010) meshing with a toothed plate (1109).

Images