A prp centrifugation system and method of making the same

Abstract

This invention relates to a PRP centrifugation system and its preparation method, belonging to the field of medical device technology. The system includes a syringe assembly, a conical centrifuge cannula, a PRP centrifuge, and a medical three-way valve. Its core improvement lies in the following: the conical centrifuge cannula is designed with a long cavity capable of accommodating a full standard syringe. The syringe is securely fixed in both directions through internal steps and grooves, eliminating the need for breakage or disassembly and ensuring a completely sealed operation, thus preventing the risk of contamination. The cannula cap adopts a conical design, combined with an open suspension support inside the centrifuge, allowing multiple cannulas to interlock and tightly converge during centrifugal rotation, significantly reducing the required centrifuge chamber diameter, thereby accommodating large-capacity syringes while maintaining equipment miniaturization. This system and method have the advantages of simple operation, high safety, and stable separation quality, effectively solving the problems of cumbersome operation, easy contamination, and difficulty in balancing equipment size and processing capacity in existing technologies.

Description

Technical Field

[0001] This invention relates to a centrifugation system, and more particularly to a concentrated platelet centrifugation system and its preparation method. Background Technology

[0002] Platelet-concentrated therapy (EPT) is a promising regenerative medicine treatment. Platelet concentrates have many names, such as platelet-rich plasma (PRP), platelet-rich fibrin (PRF), and concentrated growth factor (CGF). These clinically commonly used platelet concentrates are generated by concentrating autologous blood through centrifugation. They are rich in high concentrations of platelets and their secreted growth factors and cytokines, such as PDGF, epidermal growth factor (EGF), insulin-like growth factor-1 (IGF-1), and VEGF. These growth factors participate in cell proliferation, matrix remodeling, and angiogenesis, promoting cell recruitment, proliferation, and maturation for the regeneration of tendons, ligaments, nerves, bones, and cartilage.

[0003] Currently, the most common method for preparing autologous platelet concentrates such as PRP, PRF, and CGF, both domestically and internationally, is extraction via centrifugation. Existing technologies typically involve collecting blood with a syringe and then centrifuging for extraction, but... Figure 1-2 As shown, existing methods have the following drawbacks: ① Due to the limited height of the centrifuge chamber in existing medical centrifuges, centrifugation is often achieved by forcibly breaking the syringe plunger. This process can lead to blood entering or even overflowing the syringe stopper, wasting blood and causing contamination. ② Furthermore, existing centrifuge tubes cannot be well matched with syringe sizes, often requiring manual modification of the tubes or syringes. This necessitates separate blood draws using 5ml or 10ml syringes, resulting in a lack of stable and accurate matching. This easily leads to PRP, PRF, or CGF extraction failures or poor extraction results, and may even damage the tubes or syringes during centrifugation, causing blood leakage. ③ Different syringes vary in shape and size, but operators often fail to consider these differences during extraction, resulting in highly unstable extraction quality. Platelet concentrate extractors are disposable sterile medical devices; improper operators may reuse them, posing a significant safety hazard to users. Summary of the Invention

[0004] The purpose of this invention is to provide a PRP centrifugation system and its preparation method. The system has the advantages of simple structure, reasonable design, and the conical centrifuge sleeve included in the system can directly contain a whole 20ml syringe without breaking the plunger, avoiding blood leakage and contamination. It can be matched with 20ml syringes, making the operation more convenient and ensuring stable extraction quality. This invention solves the problems mentioned in the above-mentioned technical background.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a PRP centrifugation system, comprising: A syringe assembly comprising a plurality of first syringes and second syringes, the first syringes being used to contain plasma to be centrifuged, and the second syringes being used to contain prepared PRP; A centrifuge cannula assembly, the centrifuge cannula assembly comprising a plurality of conical centrifuge cannulas, the conical centrifuge cannulas being used to accommodate the first syringe; PRP centrifuge, the PRP centrifuge being used to drive the centrifuge sleeve assembly to centrifuge, causing the plasma in the first syringe to separate into layers; A medical three-way valve, used for extracting and transferring centrifuged and separated plasma.

[0006] Preferably, the PRP centrifuge includes a centrifuge chamber, in which a rotating centrifuge support is provided. The centrifuge support is connected to a drive mechanism. The centrifuge support is symmetrically arranged with multiple mounting structures in a circumferential direction, and the conical centrifuge sleeve is mounted on the mounting structures.

[0007] Preferably, the centrifugal support includes a base, multiple support rods, multiple support parts, and a mounting structure. The base is fixedly connected to the drive shaft of the drive structure. The multiple support rods are symmetrically distributed circumferentially. The support rods are fixedly connected to the base and are on the same plane. The multiple support parts correspond one-to-one with the support rods. The support parts are fixedly connected to the support rods and are perpendicular to each other. A mounting protrusion is provided above the support part. The mounting protrusions facing each other on two adjacent support parts constitute a mounting structure. The mounting structure is used to mount the conical centrifugal sleeve.

[0008] Preferably, the conical centrifuge cannula includes a cannula body and a cannula cap disposed on the cannula body. Both the cap and the cannula body are hollow cavity structures with an opening at one end. The cavity opening of the cap faces the cavity opening of the cylindrical tube. A receiving cavity is formed between the cannula body and the cannula cap. The receiving cavity is used to accommodate the first syringe, and both ends of the first syringe abut against the cannula body and the cannula cap, respectively.

[0009] Preferably, the interior of the cannula cavity is provided with a step, and the outer edge of the first syringe abuts against the step.

[0010] Preferably, a groove is provided below the step, and the bottom of the groove is used to abut against the handle of the syringe.

[0011] Preferably, the head of the sleeve cap is a smooth conical shape, and the apex angle of the cone is less than 90 degrees.

[0012] Preferably, the medical three-way valve has three screw-in connectors, and the first syringe and the second syringe are both screw-in syringes, which are connected to the screw-in connectors.

[0013] Preferably, one of the threaded connectors of the medical three-way valve is provided with a detachable three-way valve plug, which can be connected to the first syringe.

[0014] This invention also provides a method for preparing PRP, comprising the following steps: After drawing anticoagulant using the No. 1 syringe, blood is collected, shaken well, sealed, inserted into the conical centrifuge cannula, and fixed to the centrifuge mounting section. Perform the first centrifugation to separate the PRP from the plasma; Install a three-way valve at the inlet of syringe No. 1 and connect it to syringe No. 2 to transfer the upper plasma into syringe No. 2. The second syringe was centrifuged a second time to separate the PRP from the plasma again. Install a three-way valve at the inlet of syringe #2 and connect it to syringe #3. Transfer the plasma into syringe #3. The remaining contents in syringe #2 are PRP. Connect the three-way valve to the second syringe and transfer the remaining PRP and plasma in the first syringe (No. 3) to the second syringe for later use.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: 1. This invention provides a PRP centrifugation system with a simple overall structure and reasonable design. The centrifuge support and centrifuge chamber height of the centrifuge are improved. When the conical centrifuge sleeve is installed in the centrifuge and rotates, multiple sleeves are brought together. The conical part of the conical head can make room, reducing the overall diameter and thus reducing the volume of the centrifuge chamber and the overall size requirements of the equipment. This allows the sleeve to be made longer, so that the entire syringe can be put in without breaking the syringe, avoiding blood contamination, ensuring stable extraction quality, and directly matching a 20ml syringe for use. It eliminates the need to use 5ml or 10ml syringes to draw blood for centrifugation each time, making the operation more convenient.

[0016] 2. The conical centrifuge sleeve of the present invention has a boss and a groove in the internal cavity, which can abut against the outer edge and the handle of the syringe, making it easier and more stable to insert the syringe. Both ends of the syringe remain fixed, ensuring the stability of the internal structure of the conical centrifuge sleeve and making it more stable during centrifugation.

[0017] 3. In the preparation method of the present invention, the stopcock cap of the three-way valve is used as the plug of the syringe and the three-way valve is used to transfer the liquid, which saves costs and can also seal the syringe, making centrifugation convenient. Attached Figure Description

[0018] Figure 1 This is a schematic diagram of the internal structure of the centrifuge chamber in a PRP centrifuge, a technology currently in use.

[0019] Figure 2 This is a schematic diagram of the cross-sectional structure of the existing PRP centrifugal sleeve.

[0020] Figure 3 This is a three-dimensional schematic diagram of the PRP centrifuge system according to an embodiment of the present invention.

[0021] Figure 4 This is a schematic diagram of the centrifuge support and conical centrifuge sleeve of the PRP centrifuge system according to an embodiment of the present invention.

[0022] Figure 5 This is a schematic diagram of the centrifugal support and conical centrifugal sleeve under centrifugal conditions according to an embodiment of the present invention.

[0023] Figure 6 This is a schematic diagram of the conical centrifugal sleeve of the present invention.

[0024] Figure 7 This is a schematic cross-sectional view of the conical centrifugal sleeve of the present invention. Detailed Implementation

[0025] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] In the description of the embodiments of the present invention, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," and "outer," etc., indicating orientation or positional relationships, are based on the orientation or positional relationships shown in the accompanying drawings and are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, or be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of indicated technical features. Thus, a feature defined with "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the embodiments of the present invention, "a plurality of" means two or more, unless otherwise explicitly specified.

[0027] In the embodiments of the present invention, unless otherwise explicitly specified and limited, the terms "installation," "connection," "linking," "fixing," etc., should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, or an integral part; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; they can refer to the internal communication of two components or the interaction between two components. Those skilled in the art can understand the specific meaning of the above terms in the embodiments of the present invention according to the specific circumstances.

[0028] Example 1 like Figures 3 to 7 As shown in the figure, this embodiment describes in detail an optimized PRP centrifugation system, the core of which is to achieve a highly efficient unification of fully closed operation, large-capacity sample processing and equipment miniaturization through structural innovation.

[0029] A PRP centrifuge system, such as Figure 3 As shown, it includes: A syringe assembly (standard part not shown) includes multiple first syringes and second syringes. The first syringes are used to contain plasma to be centrifuged, and the second syringes are used to contain prepared PRP. Specifically: First Syringe: As the core processing container, this embodiment preferably uses a 20ml disposable screw-cap syringe. Its size is chosen based on the blood volume required for common clinical PRP preparation (usually 10-20ml of whole blood), and a single syringe can meet the standard treatment volume requirement, avoiding the operational complexity and interface contamination risks associated with using multiple small-volume syringes. The syringe barrel is made of highly transparent medical-grade polypropylene (PP) material, facilitating observation of the layered interface after centrifugation. Its tail handle and head sheath are reinforced to withstand centrifugal force and ensure a secure fit with the cannula.

[0030] Second syringe: As the final product container, a 5ml or 10ml screw-top syringe can be selected to hold and ultimately inject the concentrated PRP. Its smaller capacity facilitates precise injection in clinical practice.

[0031] Centrifuge sleeve assembly, the centrifuge sleeve assembly including multiple conical centrifuge sleeves 4, such as Figure 6 As shown, their number matches the mounting positions of the centrifuge (4 in this example).

[0032] The conical centrifuge sleeve 4 is used to accommodate the first syringe. This sleeve is a key adapter connecting the syringe and the centrifuge, undertaking multiple functions such as fixing, protecting, and optimizing the separation interface. The conical centrifuge sleeve 4 includes a sleeve body 41 and a sleeve cap 42 disposed on the sleeve body 41. Both the sleeve cap 42 and the sleeve body 41 are hollow cavity structures with an opening at one end. The cavity opening of the cap faces the cavity opening of the cylindrical tube. A receiving cavity is formed between the sleeve body and the sleeve cap. The receiving cavity is used to accommodate the first syringe, and both ends of the first syringe abut against the sleeve body and the sleeve cap, respectively. The head 421 of the sleeve cap is a smooth conical shape, and the apex angle of the cone is less than 90 degrees.

[0033] The interior of the cannula body 41 is provided with a step 411, and the rolled edge of the outer sleeve of the first syringe abuts against the step 411. A groove 412 is provided below the step, and the bottom 413 of the groove is used to abut against the handle of the syringe.

[0034] PRP centrifuge 1 is a dedicated PRP centrifuge: the PRP centrifuge 1 is used to drive the centrifuge cannula assembly to centrifuge, causing the plasma in the first syringe to separate into layers; the PRP centrifuge 1 includes a centrifuge chamber 2, and a rotating centrifuge support 3 is provided inside the centrifuge chamber, such as... Figure 4 As shown, the centrifugal support 3 is connected to the drive mechanism. The centrifugal support has multiple mounting structures arranged symmetrically in a circumferential direction, and the conical centrifugal sleeve 4 is mounted on these mounting structures. The centrifugal support 3 includes a base 31, multiple support rods 32, multiple support parts 33, and mounting structures. The base 31 is fixedly connected to the drive shaft of the drive structure. The multiple support rods 32 are symmetrically distributed circumferentially, fixedly connected to the base 31, and on the same plane. Each support part 33 corresponds to one of the support rods 32, and is fixedly connected to and perpendicular to the support rods 32. A mounting protrusion 34 is provided above each support part 33. The mounting protrusions 34 facing each other on two adjacent support parts 33 constitute one mounting structure, which is used to mount the conical centrifugal sleeve 5.

[0035] This centrifuge is a benchtop design optimized for PRP preparation processes. Aside from the basic drive system, its core innovation lies in the coordinated design of the centrifuge chamber 2 and the centrifuge support 3.

[0036] Medical three-way valve (standard part not shown): The medical three-way valve is used for extracting and transferring centrifuged and separated plasma. The medical three-way valve has three threaded connectors; the first syringe and the second syringe are both threaded syringes, which are connected to the threaded connectors. One of the threaded connectors of the medical three-way valve is equipped with a removable three-way valve plug, which can be connected to the first syringe.

[0037] Specifically, a standard Luer-Lock threaded three-way valve is used. The stopcock on one of its ports is designed to be fully unscrewed, and its internal thread perfectly matches the thread of the first syringe, thus serving as both a valve component and a syringe sealing plug. This reduces the need for separate plug accessories and improves the system's integration and economy.

[0038] Syringe positioning system: The cannula body is designed with an annular step inside. When the syringe is inserted, its protruding outer sheath is precisely limited by this step. Below the step, an annular groove is machined, the depth and diameter of which are calculated to perfectly accommodate and hold the handle at the end of the syringe. This dual-point axial locking mechanism of "upper sheath holding and lower handle fitting" ensures that the syringe will never slide or rotate axially during high-speed centrifugation, with minimal vibration, providing a foundation for stable plasma stratification.

[0039] Aerodynamics and Space Optimization of the Conical Head: The head of the sleeve cap is shaped into a streamlined cone with an apex angle of less than 90 degrees, preferably 55-65 degrees. This acute-angle design offers dual advantages: Reduced wind resistance: When rotating at high speed, the streamlined cone head can effectively reduce air turbulence, making the operation smoother and the noise lower.

[0040] Maximizing space utilization: such as Figure 5 As shown in the top-view diagram, when multiple cannulas are thrown outwards and converge under centrifugal force, the sharp conical tips can interlock and closely arrange themselves like wedges, significantly reducing the overall envelope diameter D of the "centrifugal bundle" composed of four cannulas. This allows the diameter of the centrifuge chamber to be designed to be smaller, thus allowing the cannulas to be long enough to fully accommodate a 20ml syringe (typically exceeding 12cm in length) without changing the overall size of the machine.

[0041] Centrifugal support structure: The support consists of a base, radial support rods, and axial support parts, welded or integrally formed. The hanging protrusion at the top of the support part forms a suspension connection with the hanging ears (or grooves) designed at both ends of the sleeve. This suspension connection allows the sleeve to be vertically suspended when stationary, and to swing freely to a horizontal centrifugal position under the action of centrifugal force after startup.

[0042] Dynamic balance and space release: The four bushings are centrally symmetrically mounted, ensuring perfect dynamic balance. More importantly, this open support configuration provides crucial space for the "convergence-embedding" behavior of the aforementioned bushings. Traditional fixed-angle rotors or high-capacity horizontal rotors require reserving maximum swing space for each bushing position, while this design utilizes the self-adjustment and mutual avoidance of the bushings to achieve dynamic sharing and efficient utilization of space.

[0043] During operation, the user simply screws on the stopper cap (i.e., the plug) of the first syringe containing the collected blood, places it directly into the conical centrifuge cannula, closes the cap, and then attaches it to the centrifuge stand to begin centrifugation. The entire process does not require touching the syringe plunger, nor does it need to be partially exposed or broken from the cannula, achieving true "insert and remove".

[0044] The overall benefits of this embodiment are: Maximum safety: No open transfers throughout the process, minimizing the risk of blood contamination and operator exposure.

[0045] Extremely simplified operation: Compatible with standard large-capacity syringes, eliminating redundant steps such as sample dispensing and plunger handling, reducing training threshold and operational error rate.

[0046] Excellent separation quality: The syringe is firmly fixed, and the conical tip helps platelets to aggregate in a very small area at the tip of the syringe at the end of centrifugation, which improves the harvest concentration and purity of PRP.

[0047] Compact and efficient equipment: Through the synergistic design of "acute-angled cone" and "open support", the ability to process large-capacity samples is realized in a small centrifuge, reducing equipment cost and space occupation.

[0048] In summary, the PRP centrifugation system provided in this embodiment resolves the contradictions between safety, convenience, capacity, and volume in the prior art through the ingenious geometric and functional matching of its components, providing a reliable and efficient PRP preparation tool for clinical use.

[0049] Example 2 A method for preparing the above-mentioned concentrated platelet centrifugation system includes the following steps: ① Take an empty syringe, which is designated as syringe number one. Use syringe number one to draw an appropriate amount of anticoagulant. Then, use syringe number one in conjunction with a blood collection needle to collect blood from the person being blooded and store it in syringe number one. Shake the blood in syringe number one. Take a medical three-way valve and remove the stopcock cap on the three-way valve as a cap to seal the syringe opening. Seal the opening of syringe number one with the cap. Place syringe number one into the cannula body 421. Tighten the cannula cap 422 on the cannula body 421 and fix the centrifuge cannula 42 on the support base 41. ② Centrifuge once in centrifuge chamber 3 to separate the PRP and plasma into layers in syringe No. 1, with the red PRP at the bottom and the pale yellow plasma at the top. After centrifugation, remove syringe No. 1 from the cannula 421, remove the cap from the syringe No. 1 port, and install a three-way valve on the syringe No. 1 port. Insert one end of the three-way valve into the syringe No. 1 port and connect the other end to an empty syringe No. 2. The medical staff operates the two syringes with both hands. Syringe No. 1 discharges plasma, and syringe No. 2 absorbs plasma. During this process, a small amount of plasma will be drawn into syringe No. 2. ③ Separate the three-way valve from the two syringes, install the matching cap on the nozzle of syringe No. 2, put it back into the cannula 421, cover and tighten the cannula cap 422, and centrifuge a second time in centrifuge chamber 3 to separate the PRP and plasma into layers again, with PRP in the lower layer and plasma in the upper layer. After centrifugation, remove syringe No. 2 from the cannula 421, remove the cap on the nozzle of syringe No. 2, install the three-way valve on the nozzle of syringe No. 2, insert one end of the three-way valve into the nozzle of syringe No. 2, and connect the other end to an empty syringe No. 3. The medical staff operate the two syringes with both hands. Syringe No. 2 discharges plasma, and syringe No. 3 absorbs plasma. The remaining components in syringe No. 2 are the prepared PRP, and the remaining components in syringe No. 3 are a small amount of PRP and a small amount of plasma. ④ Remove syringe #3, and then install an empty syringe #4 on the three-way valve in the same position as the original syringe #3. The medical staff operates the two syringes with both hands, repeatedly performing the operation of expelling and absorbing in the two syringes to mix the small amount of PRP and plasma in the original syringe #3, and keep it for later use.

[0050] Furthermore, the caps and three-way valves mentioned above are existing technologies and will not be described in detail here. All syringes are 20ml in size. PRF and CGF were also prepared using this preparation method.

[0051] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

Claims

1. A PRP centrifugation system, characterized in that, include: A syringe assembly comprising a plurality of first syringes and second syringes, the first syringes being used to contain plasma to be centrifuged, and the second syringes being used to contain prepared PRP; A centrifuge cannula assembly, the centrifuge cannula assembly comprising a plurality of conical centrifuge cannulas, the conical centrifuge cannulas being used to accommodate the first syringe; PRP centrifuge, the PRP centrifuge being used to drive the centrifuge sleeve assembly to centrifuge, causing the plasma in the first syringe to separate into layers; A medical three-way valve, used for extracting and transferring centrifuged and separated plasma.

2. The PRP centrifuge system as described in claim 1, characterized in that, The PRP centrifuge includes a centrifuge chamber, in which a rotating centrifuge support is provided. The centrifuge support is connected to a drive mechanism. The centrifuge support is symmetrically arranged in a circumferential direction with multiple mounting structures, and the conical centrifuge sleeve is mounted on the mounting structures.

3. The PRP centrifuge system as described in claim 2, characterized in that, The centrifugal support includes a base, multiple support rods, multiple support parts, and a mounting structure. The base is fixedly connected to the drive shaft of the drive structure. The multiple support rods are symmetrically distributed circumferentially. The support rods are fixedly connected to the base and are on the same plane. The multiple support parts correspond one-to-one with the support rods. The support parts are fixedly connected to the support rods and are perpendicular to each other. A mounting protrusion is provided above the support part. The mounting protrusions facing each other on two adjacent support parts constitute a mounting structure. The mounting structure is used to mount the conical centrifugal sleeve.

4. The PRP centrifuge system as described in claim 3, characterized in that, The conical centrifugal cannula includes a cannula body and a cannula cap disposed on the cannula body. Both the cannula cap and the cannula body are hollow cavity structures with an opening at one end. The cavity opening of the cannula cap is arranged facing the cavity opening of the cylindrical tube. A receiving cavity is formed between the cannula body and the cannula cap. The receiving cavity is used to accommodate the first syringe, and the two ends of the first syringe abut against the cannula body and the cannula cap, respectively.

5. The PRP centrifuge system as described in claim 4, characterized in that, The interior of the cannula cavity is provided with a step, and the outer edge of the first syringe abuts against the step.

6. The PRP centrifuge system as described in claim 5, characterized in that, The step has a groove at its bottom, and the bottom of the groove is used to abut the handle of the syringe.

7. The PRP centrifuge system as described in claim 6, characterized in that, The head of the sleeve cap is a smooth cone shape, and the apex angle of the cone is less than 90 degrees.

8. The PRP centrifuge system as described in claim 7, characterized in that, The medical three-way valve has three screw-in connectors. The first syringe and the second syringe are both screw-in syringes, and the screw-in syringes are connected to the screw-in connectors.

9. The PRP centrifuge system as described in claim 8, characterized in that, The medical three-way valve has a detachable three-way valve plug on one of its threaded connectors, and the three-way valve plug can be connected to the first syringe.

10. A method for preparing PRP, characterized in that, Includes the following steps: After drawing anticoagulant using the No. 1 syringe, blood is collected, shaken well, sealed, inserted into the conical centrifuge cannula, and fixed to the centrifuge mounting section. Perform the first centrifugation to separate the PRP from the plasma; Install a three-way valve at the inlet of syringe No. 1 and connect it to syringe No. 2 to transfer the upper plasma into syringe No.

2. The second syringe was centrifuged a second time to separate the PRP from the plasma again. Install a three-way valve at the inlet of syringe #2 and connect it to syringe #3. Transfer the plasma into syringe #3. The remaining contents in syringe #2 are PRP. Connect the three-way valve to the second syringe and transfer the remaining PRP and plasma in the first syringe (No. 3) to the second syringe for later use.

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