Anti-klh snake-derived nanobody and preparation and application thereof

By preparing nanobodies derived from black-browed snakes, the problems of large molecular weight and poor penetration of traditional anti-KLH antibodies have been solved, realizing efficient and low-cost nanobodies production and application, and expanding the application of KLH in the fields of biomedicine and diagnostics.

CN122127459APending Publication Date: 2026-06-02SOUTHWEST UNIVERSITY FOR NATIONALITIES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHWEST UNIVERSITY FOR NATIONALITIES
Filing Date
2026-03-16
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing anti-KLH antibodies have large molecular weights, poor tissue penetration, complex preparation processes, and high costs, making them difficult to use for the detection or targeted delivery of intracellular KLH-coupled antigens. Furthermore, traditional preparation methods are complex, costly, and have significant batch-to-batch variations.

Method used

High-affinity and high-specificity snake-derived nanobodies were prepared using the variable region (VH) of the heavy chain of the black-browed rat snake immunoglobulin. The nanobodies were screened using phage display technology and expressed in a prokaryotic system, providing a complete preparation method from immunization to purification.

Benefits of technology

It enables efficient, low-cost, and large-scale production of nanobodies, solving the bottlenecks of traditional antibodies in application scenarios and production processes, and providing highly sensitive detection tools and targeted delivery systems.

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Abstract

This invention relates to the field of biomedical technology, specifically to a snake-derived nanobody against KLH and its preparation and application. The invention involves immunizing the black-browed rat snake with KLH, extracting total RNA from its splenic lymphocytes, constructing a phage display VH gene library, and, after multiple rounds of screening, obtaining nanobody sequences that specifically bind to KLH, such as KLH-1 and KLH-2. This nanobody can be efficiently and solublely expressed and purified in *E. coli*, exhibiting characteristics of small molecular weight and high affinity. This invention further provides applications for this nanobody, particularly in the preparation of KLH-targeting detection reagents or drug delivery systems. This nanobody provides a core tool molecule for developing highly sensitive KLH-targeting detection reagents, efficient drug delivery systems, and novel vaccines.
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