Salt-tolerant zhanjiangibacter sp. producing isobutanol and application thereof

The Jejubacter sp. DT01 IM3 DL3 engineered bacteria, screened by heavy ion beam irradiation and MP6 plasmid mutagenesis, was introduced into the isobutanol production plasmid pDT192, solving the cytotoxicity and stress resistance problems of microbial isobutanol production and achieving high-yield and low-cost fermentation production.

CN122128147APending Publication Date: 2026-06-02BEIJING INST OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING INST OF TECH
Filing Date
2026-02-10
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing microbial methods for producing isobutanol suffer from limitations in cytotoxicity and poor stress resistance of traditional strains, resulting in low yields and high production costs, making industrial application difficult.

Method used

Jejubacter sp. DT01 IM3 DL3 was constructed by heavy ion beam irradiation mutagenesis combined with MP6 plasmid-mediated random mutagenesis screening. Isobutanol production plasmid pDT192 was introduced and combined with in-situ product separation technology to achieve efficient fermentation production.

Benefits of technology

The yield of isobutanol was significantly increased in shake flasks and fermenters, reaching 11.9 g/L and 148 g/L respectively. It has high salt tolerance and low energy consumption fermentation capacity, making it suitable for industrial production.

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Abstract

This invention belongs to the field of microbial fermentation technology, specifically relating to a high-yield isobutanol-producing Jejubacterium chassis strain, and a method for obtaining an isobutanol-producing strain and fermenting to produce isobutanol using this strain. This invention obtains Jejubacterium DT01 IM3 DL3 (CGMCC No. 30883) through heavy ion beam mutagenesis combined with MP6 plasmid-mediated random mutagenesis screening. The isobutanol-producing strain constructed using Jejubacterium DT01 IM3 DL3 as the chassis strain achieves an isobutanol yield of 11.9 g / L in shake-flask fermentation, and a super-high yield of 148 g / L was achieved in a 3L fermenter scale-up experiment. Furthermore, fermentation can be carried out at different salt concentrations (10-60 g / L), providing a possibility for industrial production using high-salt or open fermentation processes.
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