A method for rapid propagation of *Vernicia fordii* via tissue culture and its culture medium
By optimizing the culture medium formula and treatment methods for *Vernicia fordii* tissue culture, problems such as difficulty in explant disinfection, tissue browning, and difficulty in rooting have been solved, achieving efficient and safe rapid propagation of *Vernicia fordii* tissue culture, which is suitable for large-scale production.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- 河北润实生物科技有限公司
- Filing Date
- 2026-04-21
- Publication Date
- 2026-06-02
AI Technical Summary
Existing tissue culture techniques for *Vernicia fordii* suffer from difficulties in explant disinfection and severe contamination, significant tissue browning, low proliferation coefficient, difficulty in rooting, weak photosynthetic capacity of tissue culture seedlings, and fragile root systems, making it difficult to meet the needs of large-scale production.
The primary induction medium was prepared using a specific ratio of TDZ, 6-BA, reduced glutathione, and melatonin; the proliferation medium was prepared using chlorpyrifos, ZT, quercetin, and alginate oligosaccharide; and the rooting medium was prepared using IBA, NAA, nano silica, and coconut water. The explants were treated with a combination of alcohol and hydrogen peroxide silver ions for sterilization, and a gradient acclimatization transplanting method was used.
It significantly improved the survival rate and rooting rate of explants, the proliferation coefficient, and enhanced the photosynthetic capacity and stress resistance of tissue culture seedlings, ensuring efficient and safe large-scale production.
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