Material-enabled stem cell-derived exosome as well as preparation method and application thereof

By culturing mesenchymal stem cells on an N/VE-cadherin-Fc substrate and applying staged shear stress, exosomes were dynamically cultured and prepared. This solved the problem that existing exosome preparation methods could not achieve multi-target synergistic therapy, significantly improved the yield and function of exosomes, and enhanced the therapeutic effect of central nervous system injury.

CN122128226APending Publication Date: 2026-06-02NANKAI UNIV
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Patent Information

Application Number
CN202610281110.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-09
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

In the existing technology, the methods for preparing exosomes derived from mesenchymal stem cells have failed to effectively achieve multi-target synergistic treatment of central nervous system injury, lack systematic optimization of exosome preparation conditions, and the function of exosomes depends on the static effector characteristics of the mother cell, and cannot be dynamically adjusted according to changes in the lesion microenvironment.

Method used

A method combining neurovascular specific N/VE-cad-Fc substrates and dynamic culture was adopted to prepare exosomes by culturing mesenchymal stem cells on substrates coated with N/VE-cadherin-Fc fusion protein to form cadherin-enabled cell aggregates, and applying staged shear stress and dynamic culture.

Benefits of technology

It significantly increased the production of exosomes and enriched miRNAs that promote nerve regeneration, angiogenesis and anti-inflammation, achieving multi-target synergistic treatment of central nervous system injury and improving the therapeutic effect.

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Abstract

The invention discloses a material-enabled stem cell-derived exosome and a preparation method and application thereof, and belongs to the technical field of biological medicines.The preparation method comprises the following steps that mesenchymal stem cells are inoculated to an N / VE-cadherin-Fc fusion protein substrate to be cultured and then assembled to form an aggregate; applying staged shear stress to the aggregate for dynamic culture; collecting the culture medium, separating and purifying to obtain the exosome. The preparation method provided by the invention can overcome the defects of low yield and insufficient nerve and blood vessel repair function in the existing mesenchymal stem cell exosome preparation technology, obviously activates exosome biogenesis related molecular pathways, improves the exosome yield by about 4 times, is rich in nerve and blood vessel repair promoting related miRNA, and has a good application prospect. The traditional Chinese medicine composition can remarkably improve the movement function of the posterior limbs of a mouse with cerebral apoplexy and a rat with spinal cord injury, and an efficient and stable cell-free treatment strategy is provided for central nervous injury treatment.
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