A method for accurately evaluating the germplasm purity of oyster crassostrea hongkongensis

By screening for single nucleotide polymorphism sites in the whole genome of Fujian oyster using high-coverage resequencing technology, the problem of assessing the germplasm purity of Fujian oyster was solved, and the germplasm purity of Fujian oyster was accurately quantified, ensuring the controllability of germplasm purity and the stability of traits during the breeding process.

CN122128412APending Publication Date: 2026-06-02INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Filing Date
2026-03-18
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing technologies lack systematic methods for accurately assessing the germplasm purity of Fujian oysters, which fails to meet the precise and controllable requirements for the germplasm purity of Fujian oysters in aquaculture and genetic breeding.

Method used

Using high-coverage genome resequencing technology, single nucleotide polymorphism sites were screened across the entire genome of the Fujian oyster. By comparing the genome sequence with that of the Pacific oyster, a purebred Fujian oyster genome sequence site set was constructed, and the germplasm purity value was calculated, thus achieving an efficient and accurate quantitative assessment of the germplasm purity of the Fujian oyster.

Benefits of technology

This method enables precise quantitative assessment of the purity of Fujian oyster germplasm, ensuring that the genetic background and purity of parent oysters and breeding populations are controllable during the breeding process, and guaranteeing the phenotypic stability and aquaculture suitability of the selected strains.

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Abstract

This invention discloses a method for accurately assessing the germplasm purity of Fujian oysters, comprising the following steps: Step 1, acquiring high-coverage whole-genome resequencing data of the oyster individual to be tested; Step 2, comparing the resequencing data with the Fujian oyster reference genome and identifying the nucleotide base types of the individual to be tested; Step 3, assigning a score to each specific single nucleotide polymorphism site and accumulating the scores of all sites to obtain a total score; Step 4, calculating the Fujian oyster germplasm purity value of the individual to be tested based on the total score. This invention utilizes high-coverage genome resequencing to screen for single nucleotide polymorphism sites throughout the Fujian oyster genome and compares them with the genome sequence of Pacific oyster to identify highly reliable Fujian oyster genome-specific nucleotide bases, constructing a purebred Fujian oyster genome sequence site set, and achieving efficient and accurate quantitative assessment of Fujian oyster germplasm purity by calculating the percentage of purebred sites in the Fujian oyster genome.
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