Method for accurately evaluating purity of Fujian oyster germplasm

By screening for single nucleotide polymorphism sites in the whole genome of Fujian oyster using high-coverage resequencing technology, the problem of assessing the germplasm purity of Fujian oyster was solved, and the germplasm purity of Fujian oyster was accurately quantified, ensuring the controllability of germplasm purity and the stability of traits during the breeding process.

CN122128412APending Publication Date: 2026-06-02INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Patent Information

Application Number
CN202610332265.4
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-03-18
Publication Date
2026-06-02

AI Technical Summary

Technical Problem

Existing technologies lack systematic methods for accurately assessing the germplasm purity of Fujian oysters, which fails to meet the precise and controllable requirements for the germplasm purity of Fujian oysters in aquaculture and genetic breeding.

Method used

Using high-coverage genome resequencing technology, single nucleotide polymorphism sites were screened across the entire genome of the Fujian oyster. By comparing the genome sequence with that of the Pacific oyster, a purebred Fujian oyster genome sequence site set was constructed, and the germplasm purity value was calculated, thus achieving an efficient and accurate quantitative assessment of the germplasm purity of the Fujian oyster.

Benefits of technology

This method enables precise quantitative assessment of the purity of Fujian oyster germplasm, ensuring that the genetic background and purity of parent oysters and breeding populations are controllable during the breeding process, and guaranteeing the phenotypic stability and aquaculture suitability of the selected strains.

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Abstract

The invention discloses a Fujian oyster germplasm purity accurate evaluation method which comprises the following steps: step 1, acquiring high-coverage whole genome re-sequencing data of a to-be-detected oyster individual, step 2, comparing the re-sequencing data with a Fujian oyster reference genome, and identifying the nucleotide base type of the to-be-detected individual. Step 3, assigning a score to each specific mononucleotide polymorphic site, and accumulating scores of all sites to obtain a total score, and step 4, calculating the Fujian oyster germplasm purity value of the to-be-detected oyster individual according to the total score. According to the invention, genome high-coverage re-sequencing is utilized to screen single nucleotide polymorphic sites in a Fujian oyster whole genome range, the single nucleotide polymorphic sites are compared with a pacific oyster genome sequence, high-reliability Fujian oyster genome specific nucleotide bases are identified, a pure Fujian oyster genome sequence site set is constructed, and the single nucleotide polymorphic sites can be used for screening the single nucleotide polymorphic sites in the Fujian oyster whole genome range. By calculating the percentage of the purebred sites in the Fujian oyster genome, efficient and accurate quantitative evaluation of the Fujian oyster germplasm purity is realized.
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