Deodorizing agent for artificial breeding of sea horse and application thereof

Abstract

The application discloses a deodorizing agent special for artificial breeding of Hippocampus and application thereof. The active ingredient of the deodorizing agent is obtained by extraction of the following raw materials in parts by weight: 35-45 parts of wood milk fruit pulp, 18-22 parts of pepper leaves, 12-18 parts of Litsea, and 8-12 parts of liquorice extract; wherein the weight ratio of Litsea to liquorice extract is (1.5-2.2):1. The deodorizing agent is applied to the deodorization and insect prevention of Hippocampus, and the process comprises the following steps: pretreatment of Hippocampus by low-temperature plasma, then ultrasonic-assisted immersion in the deodorizing agent, and finally radio frequency vacuum drying to obtain the final product. Through the synergy of specific plant proportioning and physical processing technology, the application synchronously realizes efficient deodorization and long-acting physical insect prevention of Hippocampus, and has no chemical residues, can effectively retain active ingredients of Hippocampus, and is suitable for green processing of high-quality Hippocampus products.

Description

Technical Field

[0001] This invention belongs to the field of seafood processing technology, and specifically relates to a deodorizing agent for artificially cultured seahorses and its application. Background Technology

[0002] Seahorse is a valuable marine medicinal herb with effects such as warming the kidneys and strengthening yang, dispersing nodules and reducing swelling. However, seahorse is rich in trimethylamine, dimethylamine and other fishy-smelling substances, and even after drying, it still retains a strong fishy odor, seriously affecting the taste of its medicinal preparations and patient compliance. At the same time, dried seahorse is extremely susceptible to being eaten by storage pests (such as medicinal herb beetles and tobacco beetles) during storage, leading to damage to the medicinal material, reduced efficacy, or even complete loss of value.

[0003] Currently, there are two major, mutually exclusive technical challenges in the processing and storage of seahorses: First, deodorization mainly relies on traditional methods such as steaming with rice wine and soaking in tea, which have limited effectiveness and easily lead to the loss of active ingredients; second, insect control heavily depends on fumigation with chemical agents such as aluminum phosphide. While this method has some insect-repelling effect, it poses safety risks due to its high toxicity, flammability, explosiveness, and drug residues, contradicting the industry trend of green development. In recent years, although some studies have attempted to improve the process using plant-derived ingredients, these efforts have mostly been limited to exploring single functions (such as deodorization or insect control only), or the effects are difficult to maintain and stabilize.

[0004] While there are existing reports of applying various food processing technologies (such as low-temperature plasma, pulsed electric field, and radio frequency drying) to aquatic product processing, most focus on achieving a single objective (such as sterilization or drying). However, there are no publicly reported examples of innovatively combining a series of modern physical processing technologies with a specific plant-based compound system to simultaneously and synergistically address the long-standing industry pain points of "removing fishy odor" and "preventing insects" in seahorses.

[0005] Therefore, developing a processing method that can simultaneously achieve efficient deodorization, long-lasting insect prevention, and is green and safe while preserving the medicinal components of seahorses to the greatest extent has significant industrial value and practical significance. Summary of the Invention

[0006] In view of the above, it is necessary to provide a deodorizing agent for artificially bred seahorses and its application. By innovatively integrating technologies such as low-temperature plasma pretreatment, pulsed electric field preparation of specific plant compound extracts, ultrasonic-assisted soaking, and radio frequency vacuum drying, the fishy smell of seahorses can be efficiently removed.

[0007] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0008] A deodorizing agent specifically for artificially bred seahorses is a compound plant functional extract, mainly obtained by extracting the following raw materials in parts by weight: 35-45 parts of wood milk fruit pulp, 18-22 parts of Sichuan pepper leaves, 12-18 parts of Litsea cubeba, and 8-12 parts of licorice extract; wherein the weight ratio of Litsea cubeba to licorice extract is (1.5~2.2):1.

[0009] Another object of the present invention is to provide the application of the above-mentioned deodorizing agent for artificially bred seahorses, which is used for deodorizing and / or insect-proofing treatment of seahorses.

[0010] Another object of the present invention is to provide a method for deodorizing and preventing insect infestation of seahorses using the above-described deodorizing agent, comprising the following steps:

[0011] (1) Low-temperature plasma pretreatment: After cleaning and draining the seahorse, place it in a low-temperature plasma generator and treat it for 3-5 minutes in an air atmosphere with a discharge power of 80-120W.

[0012] (2) Deodorizing and insect-repelling soaking: Immerse the pretreated seahorse in the deodorizing agent and perform ultrasonic-assisted soaking at 10-15℃ for 2-3 hours;

[0013] (3) Radio frequency vacuum drying: After soaking, the seahorse is taken out and drained, and placed in a radio frequency vacuum drying device. It is dried at a vacuum degree of 0.08-0.09MPa and a temperature of 35-40℃ until the moisture content is 8%-12%.

[0014] Preferably, the deodorizing agent is prepared by the following steps: after mixing the raw materials, add an ethanol aqueous solution with a volume fraction of 40%-50% and a total weight of 6-8 times the total weight of the materials, and perform extraction with the assistance of a pulsed electric field. The parameters of the pulsed electric field are: electric field strength 25-35kV / cm, pulse frequency 50-70Hz, and treatment time 15-25min; the extract is obtained after fine filtration and concentration.

[0015] Preferably, in step (1), the discharge power of the low-temperature plasma pretreatment is 100W, the treatment time is 4min, and the air flow rate is 6L / min.

[0016] Preferably, in step (2), the ratio of the soaking liquid to the material is 1:5-1:7 (g / mL), the ultrasonic power is 200-300W, and the frequency is 30-40kHz.

[0017] Preferably, in step (2), the weight parts of each raw material are: 40 parts of wood milk fruit pulp, 20 parts of Sichuan pepper leaves, 15-18 parts of wood litsea cubeba, and 8-10 parts of licorice extract.

[0018] Preferably, the weight parts of each raw material are: 40 parts of wood milk fruit pulp, 20 parts of Sichuan pepper leaves, 18 parts of wood litsea cubeba, and 10 parts of licorice extract.

[0019] Another objective of this invention is to provide a seahorse product prepared by the above method. This product has an extremely low fishy odor (trimethylamine removal rate ≥92%), a high sensory score, and exhibits a strong repellent effect against common storage pests (such as the herb beetle) (repellent rate ≥95% after 60 days), with a high retention rate of active ingredients.

[0020] Compared with the prior art, the present invention has at least the following beneficial effects:

[0021] 1. This invention achieves a synergistic effect of deodorizing and insect-repelling functions by limiting the compound formulation of wood apple, Sichuan pepper leaves, litsea cubeba, and licorice extract, and controlling the weight ratio of litsea cubeba to licorice extract at (1.5~2.2):1. This is because wood apple polyphenols and licorice flavonoids can effectively decompose trimethylamine and other precursors of fishy odor, while the volatile components of Sichuan pepper leaves and litsea cubeba can mask residual fishy odor and impart a pleasant fragrance. More importantly, experimental data show that at this specific ratio (as in Examples 1 and 2), the above components have a significant synergistic repellent effect on storage pests (such as medicinal herb beetles), giving the product a long-lasting physical insect-repelling capability (60-day repellency rate ≥95%, insect infestation loss rate <5%). This effect cannot be achieved by a single component (Comparative Example 1) or a conventional compound formulation (Comparative Examples 2 and 3), thus simultaneously solving the two major technical bottlenecks of incomplete deodorization and residual chemical insect repellent within a single process system.

[0022] 2. This invention improves processing efficiency and product quality by integrating a process chain of "low-temperature plasma pretreatment—pulsed electric field enhanced extraction—ultrasound-assisted soaking—radio frequency vacuum drying." This is because low-temperature plasma pretreatment with specific parameters effectively alters the microstructure of the seahorse epidermis, significantly enhancing its permeability. This creates conditions for the subsequent extract to exert its effects quickly; specifically, the soaking time required to achieve the same deodorization rate is shortened by approximately 37.5% compared to the untreated group (Comparative Example 5). Pulsed electric field-assisted extraction significantly improves the dissolution efficiency of plant active ingredients under mild conditions. Subsequent radio frequency vacuum drying, through uniform bulk heating, rapidly dehydrates while avoiding the functional component inactivation or surface hardening problems that may occur with hot air drying, resulting in a retention rate of over 92% for the core medicinal components of seahorse (such as seahorse saponin A). The entire process is interconnected, forming a highly efficient synergy.

[0023] 3. This invention provides a complete green processing path, resulting in a final product of excellent overall quality. By employing physical processing techniques and natural plant ingredients throughout the process, it completely eliminates the use of chemical fumigants such as aluminum phosphide, thus preventing the risk of toxic and harmful substance residues from the source. The resulting seahorse product combines low fishy odor (odor removal rate ≥92%), harmonious flavor, internal insect repellency, and high retention rate of active ingredients. Its stable quality meets the multiple demands of the high-end market for the safety, efficacy, and storage stability of seahorse medicinal materials, demonstrating significant industrial application value. Detailed Implementation

[0024] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention are described in detail below. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be practiced in many other ways different from those described herein, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0025] Example 1:

[0026] This embodiment proposes a method for preparing a deodorizing agent, as follows:

[0027] Weigh out 400g of *Litsea cubeba* pulp, 200g of *Zanthoxylum bungeanum* leaves, 180g of *Litsea cubeba* seeds, and 100g of licorice extract. Grind the pulp, leaves, and seeds separately to 60 mesh and mix evenly with the licorice extract (weight ratio of *Litsea cubeba* seeds to licorice extract is 1.8:1). Add 8 times the total weight of the materials to a 45% (v / v) edible ethanol aqueous solution, and place the mixture in a pulsed electric field extraction device. Treat for 20 min at an electric field strength of 30 kV / cm and a pulse frequency of 60 Hz. Then transfer the mixture to a constant temperature stirring tank and extract by stirring at 48℃ and 120 r / min for 2 h. Coarsely filter the extract through a 100-mesh sieve, then finely filter through a 0.22 μm organic filter membrane, and finally concentrate under reduced pressure at 45℃ and -0.085 MPa to 1 / 4 of the original volume to obtain a compound plant functional extract, which is the deodorizing agent for artificially bred seahorses described in this invention, for later use.

[0028] This embodiment also proposes a method for treating seahorses using the above-mentioned deodorizing agent, including the following steps:

[0029] a. Pretreatment: Take 1 kg of fresh lined seahorse, remove the internal organs, wash 3 times with sterile water at 3℃, and drain. Place it in a low-temperature plasma generator, set the discharge power to 100 W, the air flow rate to 6 L / min, and treat for 4 min.

[0030] b. Deodorizing and insect-repelling soaking: Immerse the pretreated seahorse in the deodorizing agent prepared in step (1) (material-liquid ratio of 1:6, g / mL), place it in an ultrasonic cleaning tank at 12℃, set the ultrasonic power to 250 W and the frequency to 35 kHz, soak for 2.5 h, and manually stir for 3 min every 30 min during the soaking period.

[0031] c. Drying: Remove the seahorses and drain the surface liquid. Place them in an RF vacuum dryer, set the RF power to 1.5kW, the vacuum degree to 0.085 MPa, and the drying temperature to 38℃, and dry until the moisture content of the seahorses is 10.5%. Cool to room temperature, seal and package to obtain the final seahorse product.

[0032] Example 2:

[0033] This embodiment proposes a method for preparing a deodorizing agent, as follows:

[0034] Weigh out 350g of *Litsea cubeba* pulp, 180g of *Zanthoxylum bungeanum* leaves, 150g of *Litsea cubeba* seeds, and 100g of licorice extract. Grind the plant materials to 50 mesh and mix thoroughly (the weight ratio of *Litsea cubeba* seeds to licorice extract is 1.5:1). Add 6 times the total weight of the materials to a 40% (v / v) edible ethanol aqueous solution. Place the mixture in a pulsed electric field extraction device and treat for 25 min at an electric field strength of 25 kV / cm and a pulse frequency of 50 Hz. Then transfer the mixture to a constant temperature stirred tank and extract at 45℃ and 100 r / min for 2.5 h. Coarsely filter the extract through a 100-mesh sieve, then finely filter through a 0.22 μm organic filter membrane, and finally concentrate under reduced pressure at 45℃ and -0.09 MPa to obtain a compound plant functional extract for later use.

[0035] This embodiment also proposes a method for treating seahorses using the above-mentioned deodorizing agent, including the following steps:

[0036] a. Pretreatment: Take 1 kg of fresh lined seahorse, wash and drain. Place it in a low-temperature plasma generator, set the discharge power to 80 W, the air flow rate to 5 L / min, and treat for 5 min.

[0037] b. Deodorizing and insect-repelling soaking: Immerse the pretreated seahorse in the deodorizing agent prepared in step (1) (the material-to-liquid ratio is 1:5 (g / mL)), place it in an ultrasonic cleaning tank at 10℃, set the ultrasonic power to 200 W and the frequency to 30 kHz, soak for 3 h, and stir regularly during the process.

[0038] c. Drying: Remove the seahorses and drain them. Place them in an RF vacuum dryer, set the RF power to 1.2 kW, the vacuum degree to 0.09 MPa, and the drying temperature to 35°C, and dry until the moisture content of the seahorses is 12%. After cooling, seal and package them.

[0039] Example 3:

[0040] This embodiment proposes a method for preparing a deodorizing agent, as follows:

[0041] Weigh out 450g of *Litsea cubeba* pulp, 220g of *Zanthoxylum bungeanum* leaves, 180g of *Litsea cubeba* seeds, and 82g of licorice extract. Grind the plant materials to 70 mesh and mix thoroughly (the weight ratio of *Litsea cubeba* seeds to licorice extract is approximately 2.2:1). Add 8 times the total weight of the materials to a 50% (v / v) edible ethanol aqueous solution, and place the mixture in a pulsed electric field extraction device. Treat for 15 min at an electric field strength of 35 kV / cm and a pulse frequency of 70 Hz. Then transfer the mixture to a constant temperature stirred tank and extract by stirring at 50℃ and 130 r / min for 1.5 h. Coarsely filter the extract through a 100-mesh sieve, then finely filter through a 0.22 μm organic filter membrane, and concentrate under reduced pressure at 45℃ and -0.08 MPa to obtain a compound plant functional extract for later use.

[0042] This embodiment also proposes a method for treating seahorses using the above-mentioned deodorizing agent, including the following steps:

[0043] a. Pretreatment: Take 1 kg of fresh lined seahorse, wash and drain. Place it in a low-temperature plasma generator, set the discharge power to 120 W, the air flow rate to 8 L / min, and treat for 3 min.

[0044] b. Deodorizing and insect-repelling soaking: Immerse the pretreated seahorse in the deodorizing agent prepared in step (1) (the material-to-liquid ratio is 1:7 (g / mL)), place it in an ultrasonic cleaning tank at 15℃, set the ultrasonic power to 300 W and the frequency to 40 kHz, soak for 2 h, and stir regularly during the process.

[0045] c. Drying: Remove the seahorses and drain them. Place them in an RF vacuum dryer, set the RF power to 1.8 kW, the vacuum degree to 0.08 MPa, and the drying temperature to 40°C, and dry until the moisture content of the seahorses is 8%. After cooling, seal and package them.

[0046] Comparative Example 1: Single-component deodorizing agent

[0047] The only difference between this comparative example and Example 1 is the raw material used in the deodorizing agent preparation step: only 400g of *Lysimachia christinae* pulp is used, without the addition of Sichuan pepper leaves, *Litsea cubeba*, and licorice extract. All other steps and parameters are exactly the same as in Example 1.

[0048] Comparative Example 2: Conventional proportion of plant-based deodorizing agent

[0049] The only difference between this comparative example and Example 1 is the ratio of raw materials in the deodorizing agent preparation step: 400g of Litsea cubeba pulp, 200g of Sichuan pepper leaves, 100g of Litsea cubeba fruit, and 100g of licorice extract were weighed (the weight ratio of Litsea cubeba fruit to licorice extract was 1:1). The remaining steps and parameters were exactly the same as in Example 1.

[0050] Comparative Example 3: The proportion is slightly less than the scope of this invention.

[0051] The only difference between this comparative example and Example 1 is the ratio of raw materials in the deodorizing agent preparation step: 400g of Litsea cubeba pulp, 200g of Sichuan pepper leaves, 140g of Litsea cubeba fruit, and 100g of licorice extract were weighed (the weight ratio of Litsea cubeba fruit to licorice extract was 1.4:1). The remaining steps and parameters were exactly the same as in Example 1.

[0052] Comparative Example 4: Traditional Chemical Insect Control Methods

[0053] This comparative example represents the prior art. The deodorizing agent and method of this invention are not used. One kilogram of fresh lined seahorses from the same source were processed and directly placed in a 60°C hot air drying oven to dry to a moisture content of 10.5%. After drying, the dried seahorses were placed together with aluminum phosphide tablets in a sealed fumigation chamber and fumigated for 48 hours for insect prevention. Afterward, the chamber was ventilated and sealed in packaging.

[0054] Comparative Example 5: Low-temperature plasma pretreatment omitted

[0055] The difference between this comparative example and Example 1 is that the low-temperature plasma pretreatment step is omitted. After the seahorses are cleaned and drained, they are directly soaked for deodorization and insect prevention. To achieve a deodorization effect similar to that of Example 1, preliminary experiments determined that the ultrasonic-assisted soaking time needs to be extended to 4.0 hours. The remaining steps and parameters are the same as in Example 1.

[0056] Experimental Design and Methods

[0057] To systematically verify and determine the optimal formulation and effect of the deodorizing agent of the present invention, the following experiments were conducted, with three parallel samples set for each experiment.

[0058] 1. Formula optimization experiment:

[0059] Single-factor experiment: The addition amounts of the other three ingredients were fixed at the intermediate level (35 parts of wood apple pulp, 18 parts of Sichuan pepper leaves, 12 parts of Litsea cubeba, and 8 parts of licorice extract), and the effects of the addition amount of each single ingredient within its set range on the deodorizing effect were investigated.

[0060] Orthogonal Experiment: Based on the results of the single-factor experiment, four factors were selected: fruit pulp (A), Sichuan pepper leaves (B), Litsea cubeba (C), and licorice extract (D). Each factor had three levels, and an L9(3) orthogonal experimental design was adopted. 4An orthogonal array was used to design experiments, with the odor removal rate as the core indicator, to determine the optimal formula combination. Verification experiments confirmed the reliability of the optimal formula.

[0061] 2. Effect comparison and verification experiment:

[0062] The optimal formula (i.e., the formula of Example 1) and its boundary ratios (Examples 2 and 3) determined by the above experiments were used in parallel with multiple comparative examples to compare their overall effects.

[0063] 3. Evaluation indicators and measurement methods:

[0064] Odor removal rate: The trimethylamine (TMA) content in seahorse samples was determined using the Kjeldahl method. The odor removal rate was calculated as follows: [(TMA content of untreated seahorse - TMA content of treated seahorse) / TMA content of untreated seahorse] × 100%.

[0065] Sensory evaluation: A scoring panel of 10 trained professional evaluators conducted a blind evaluation using a 10-point scale (0 points: extremely strong fishy smell, unacceptable; 10 points: no fishy smell, harmonious flavor), and the average value of the results was taken.

[0066] Insect repellency performance: Referring to GB / T 31762-2015 "Determination Method for Repellent Effect of Pests in Chinese Medicinal Materials", Stegobium paniceum (a typical pest damaging dried Chinese medicinal materials such as seahorse) was used as the test insect. The repellency rate was measured after 48 hours. Simultaneously, the samples were stored at 28±1℃ and 75±5%RH for 60 days, and the insect infestation loss rate was calculated.

[0067] Retention rate of active ingredients: The content of the main active ingredient, seahorse saponin A, in seahorse products was determined by high performance liquid chromatography (HPLC), and the retention rate was calculated based on fresh seahorse.

[0068] Results and Analysis

[0069] 1. Analysis of deodorization effect: The treatment effects of each embodiment and comparative example are shown in Table 1.

[0070] Table 1: Comparison of the deodorizing effects of different treatment methods on seahorses

[0071]

[0072] As shown in Table 1, the seahorses treated using the method of this invention (Examples 1-3) exhibited significantly better deodorization and flavor harmony than those treated using the traditional method (Comparative Example 4). This is likely because the active ingredients such as polyphenols and flavonoids in the compound plant extracts underwent decomposition or binding reactions with the fishy substances such as trimethylamine in the seahorse, while the volatile components in Sichuan pepper leaves and Litsea cubeba effectively masked and harmonized the residual fishy odor. The decreased effect of Comparative Example 1 (single component) indicates the necessity of combining multiple functional components. The deodorization rate of Examples 1-3 (ratios between 1.5:1 and 2.2:1) remained stable above 91.5%, with superior sensory scores, while the effects of Comparative Examples 2 and 3 (ratios of 1:1 and 1.4:1, respectively) decreased, indicating that the ratio of Litsea cubeba to licorice extract has a significant impact on the final deodorization effect and flavor balance. Comparative Example 5, without low-temperature plasma pretreatment, achieved a similar deodorization rate (92.1%) by extending the soaking time to 4.0 hours, but its treatment efficiency was significantly lower than that of Example 1 (soaking for 2.5 hours).

[0073] 2. Insect-repellent effect analysis: The insect-repellent performance of each embodiment and comparative example is shown in Table 2.

[0074] Table 2: Comparison of insect-repellent effects of seahorse products treated with different methods

[0075]

[0076] Note: Comparative Example 4 relies on chemical fumigation, and the insect-proof effect is directly caused by chemical pesticides, which poses safety and residue risks.

[0077] The insect repellency test results (Table 2) show that the seahorse product treated according to the present invention exhibits a significant repellent effect against *Litsea cubeba*. This is likely because volatile active substances in plant extracts (such as citral in *Litsea cubeba* and linalool in *Zanthoxylum bungeanum* leaves) remain on the surface of the seahorse, collectively creating an environment disliked by pests. Examples 1, 2, and 3 (with *Litsea cubeba* and licorice in the same ratio as claimed) showed excellent and long-lasting insect repellency, with repellency rates all exceeding 92% and extremely low insect damage rates. In contrast, the insect repellency performance of Comparative Example 2 (1:1) and Comparative Example 3 (1.4:1) significantly decreased with changes in the ratio. This trend suggests that, under specific ratios, the insect-repellent active ingredients of *Litsea cubeba* and licorice extracts may produce a more optimized combination or release effect. Furthermore, the present invention (Example 1) achieves insect repellency comparable to chemical fumigation (Comparative Example 4) while avoiding the inherent chemical residue risks of the latter.

[0078] 3. Process efficiency and active ingredient retention analysis: The results are shown in Table 3.

[0079] Table 3: Comparison of Process Efficiency and Retention of Active Ingredients

[0080]

[0081] The results show that Example 1, employing the complete process chain of this invention, reduced the soaking time by 37.5% compared to Comparative Example 5 (which omitted plasma pretreatment). This indicates that low-temperature plasma pretreatment significantly altered the surface properties of the seahorse, greatly enhancing the penetration and effectiveness of the subsequent deodorizing agent, demonstrating a synergistic effect between process steps. Furthermore, the invention utilizes a gentle processing method throughout, resulting in a significantly higher retention rate of seahorse saponin A (92.3%) compared to traditional chemical insect control methods (Comparative Example 4, 85.4%), effectively protecting the active pharmaceutical ingredient.

[0082] The experimental data above fully demonstrate that this invention, through the use of a specific compound plant formula, produces an unexpected synergistic effect. It not only achieves highly efficient deodorization (removal rate >91%), but also simultaneously endows seahorse products with excellent long-lasting physical insect-repellent properties (repellency rate >92%, 60-day insect infestation loss rate <5%), thus successfully solving the industry problem of the incompatibility between "deodorization" and "chemical insect control" in traditional technologies. Furthermore, by integrating the innovative process chain of "low-temperature plasma pretreatment - pulsed electric field extraction - radio frequency vacuum drying," it significantly improves overall processing efficiency and maximizes the preservation of the inherent active ingredients of seahorse while ensuring efficient extraction and activity retention of functional components. In addition, this technical solution completely avoids the chemical residue risks of traditional aluminum phosphide fumigation, providing a green, safe, and efficient integrated solution for deodorization and insect control of seahorse, aligning with the development trend of high-quality Chinese medicinal material processing.

[0083] The above embodiments are merely examples of several implementations of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the present invention.

Claims

1. A deodorizing agent specifically for artificially cultured seahorses, characterized in that, It is a compound plant functional extract, mainly obtained by extracting the following raw materials in parts by weight: 35-45 parts of wood milk fruit pulp, 18-22 parts of Sichuan pepper leaves, 12-18 parts of Litsea cubeba, and 8-12 parts of licorice extract; wherein, the weight ratio of Litsea cubeba to licorice extract is (1.5~2.2):

1.

2. The application of the deodorizing agent specifically for artificially bred seahorses according to claim 1, characterized in that, It is used for deodorizing and / or insect-repelling treatment of seahorses.

3. A method for deodorizing and insect-repelling seahorses using the deodorizing agent according to claim 1, characterized in that, The method includes the following steps: (1) Low-temperature plasma pretreatment: After cleaning and draining the seahorse, place it in a low-temperature plasma generator and treat it for 3-5 minutes in an air atmosphere with a discharge power of 80-120W. (2) Deodorizing and insect-repelling soaking: Immerse the pretreated seahorse in the deodorizing agent and perform ultrasonic-assisted soaking at 10-15℃ for 2-3 hours; (3) Radio frequency vacuum drying: After soaking, the seahorse is taken out and drained, and placed in a radio frequency vacuum drying device. It is dried at a vacuum degree of 0.08-0.09MPa and a temperature of 35-40℃ until the moisture content is 8%-12%.

4. The method according to claim 3, characterized in that, The deodorizing agent is prepared by the following steps: after mixing the raw materials according to claim 1 by weight, add an ethanol aqueous solution with a volume fraction of 40%-50% and a total weight of 6-8 times the total weight of the materials, and perform assisted extraction using a pulsed electric field. The parameters of the pulsed electric field are: electric field strength 25-35kV / cm, pulse frequency 50-70Hz, and treatment time 15-25min; the extract is obtained after fine filtration and concentration.

5. The method according to claim 3 or 4, characterized in that, In step (1), the discharge power of the low-temperature plasma pretreatment is 100W, the treatment time is 4min, and the air flow rate is 6L / min.

6. The method according to claim 3 or 4, characterized in that, In step (2), the ratio of material to liquid for soaking is 1:5-1:7 (g / mL), the ultrasonic power is 200-300W, and the frequency is 30-40kHz.

7. The method according to claim 4, characterized in that, In step (2), the weight parts of each raw material are: 40 parts of wood milk fruit pulp, 20 parts of Sichuan pepper leaves, 15-18 parts of wood litsea cubeba, and 8-10 parts of licorice extract.

8. The method according to claim 7, characterized in that, The weight proportions of each ingredient are as follows: 40 parts of wood milk fruit pulp, 20 parts of Sichuan pepper leaves, 18 parts of wood litsea cubeba, and 10 parts of licorice extract.

9. A seahorse product, characterized in that, It is prepared by the method described in any one of claims 3-8.

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