A traditional Chinese medicine composition for promoting wound healing and a preparation method thereof

Abstract

The present application relates to the technical field of wound repair, and provides a traditional Chinese medicine composition for promoting wound healing and a preparation method thereof.The traditional Chinese medicine composition comprises a first traditional Chinese medicine composition and a second traditional Chinese medicine composition which are applied to the acute phase and the repair proliferation phase.The former comprises naked flower purple perilla extract, nectarine leaf extract, purple perilla extract, dragon's blood micro powder, spironast extract, centella asiatica volatile oil, rhubarb extract, borneol, mussel mucin, medicinal glycerol and purified water.The latter comprises red algae extract, nectarine leaf extract, radix astragali extract, panax notoginseng total saponins, aloe extract, dendrobium officinale extract, sea buckthorn extract, pinctada martensii purified peptide, snail glycosaminoglycan, medicinal glycerol and purified water.The composition can play the roles of antibiosis, anti-inflammation, hemostasis, analgesia and astringency in the acute phase, and can promote angiogenesis, collagen synthesis and epithelial regeneration in the repair proliferation phase, so that the wound is rapidly repaired and healed.

Description

Technical Field

[0001] This invention relates to the technical field of wound repair, and provides a traditional Chinese medicine composition for promoting wound healing and its preparation method. Background Technology

[0002] Wound healing is a complex biological process, typically involving four consecutive stages: hemostasis, inflammation, proliferation, and remodeling. Abnormalities in any of these stages can lead to delayed healing, worsened infection, excessive scarring, and even the formation of chronic, intractable wounds. Targeted drug interventions for wound repair can exert antibacterial and anti-inflammatory effects, stop bleeding and promote wound healing, facilitate granulation and epithelial regeneration, improve the healing microenvironment, shorten healing time, improve healing quality, and reduce scarring and infection risks.

[0003] Medications that promote wound healing include antibacterial and disinfectant drugs, healing-promoting or growth factor drugs, hemostatic drugs, scar-reducing or anti-proliferative drugs, and wound-protecting or moist wound-healing drugs. Among these, traditional Chinese medicine or natural drugs are commonly used in wound repair, exerting good effects in clearing heat and detoxifying, antibacterial and anti-inflammatory, astringent and hemostatic, wound-healing, swelling-reducing and pain-relieving, blood-activating and stasis-removing, tissue-regenerating and wound-closing, and promoting healing.

[0004] Compared with Western medicine, the advantages of traditional Chinese medicine are: it can be compounded to exert a synergistic effect of multiple components; it is less likely to produce drug resistance and has a low risk of long-term use; it has good stability and safe sources. However, the disadvantage of traditional Chinese medicine is that it takes longer to take effect.

[0005] Furthermore, wound healing occurs in stages. In the early stages, the wound is open, with significant bleeding and exudation, making it susceptible to infection, and causing obvious redness, swelling, heat, and pain. At this stage, the focus should be on stopping bleeding and relieving pain, administering antibacterial and anti-inflammatory treatments, and protecting the wound surface. In the middle and later stages, as inflammation subsides and new tissue begins to grow, with epithelial growth, the focus should be on promoting new tissue regeneration and healing. Therefore, fixed-component traditional Chinese medicine cannot effectively target these different stages, which is detrimental to the healing speed. Summary of the Invention

[0006] In view of the above, this invention proposes a traditional Chinese medicine composition for promoting wound healing and its preparation method. It can play a good role in antibacterial, anti-inflammatory, hemostatic, analgesic and astringent effects in the acute phase, providing a good environment for rapid wound repair and healing. In the repair and proliferation phase, it effectively promotes angiogenesis, collagen synthesis and epithelial regeneration, thereby achieving rapid wound repair and healing.

[0007] To achieve rapid and efficient wound healing, the specific technical solution involved in this invention is as follows: The first part of this invention provides a traditional Chinese medicine composition for promoting wound healing and a method for preparing the same.

[0008] The traditional Chinese medicine composition comprises two stages of sub-compositions, namely a first traditional Chinese medicine composition and a second traditional Chinese medicine composition.

[0009] The first traditional Chinese medicine composition includes extracts of Callicarpa nudiflora, Nectarine leaf extract, Callicarpa japonica extract, Dragon's blood powder, Portulaca oleracea extract, Centella asiatica volatile oil, Rheum palmatum extract, Borneol, Mussel adhesive protein, medicinal glycerin, and purified water; The second traditional Chinese medicine composition includes red algae extract, nectarine leaf extract, astragalus extract, total saponins of Panax notoginseng, aloe extract, Dendrobium officinale extract, sea buckthorn extract, purified peptides of Pinctada martensii, snail glycosaminoglycans, pharmaceutical glycerin, and purified water.

[0010] Wound healing typically involves three stages: First, the acute phase, where the wound is open, with significant bleeding and exudation, making it susceptible to infection, and characterized by pronounced redness, swelling, heat, and pain. The core objectives of drug intervention in this phase are hemostasis, pain relief, infection control, exudation control, and swelling and inflammation reduction. Second, the repair and proliferation phase, where new tissue begins to grow and epithelial cells spread. The core objectives of drug intervention in this phase are to promote granulation tissue growth, angiogenesis, accelerate epithelial healing, and promote moist healing. Third, the remodeling and maturation phase, where the wound has largely healed but is prone to scarring, pigmentation, and tightness. The core objectives of drug intervention in this phase are to reduce scar hyperplasia, lighten pigmentation, repair the skin barrier, and prevent recurrence. The herbal composition of this invention primarily promotes wound healing, targeting the first two stages.

[0011] The first traditional Chinese medicine composition is suitable for the acute phase. It combines extracts of *Callicarpa nudiflora*, *Callicarpa japonica*, *Dracaena cochinchinensis* micron powder, *Portulaca oleracea* extract, *Centella asiatica* volatile oil, and *Rheum palmatum* extract, which have good antibacterial, anti-inflammatory, hemostatic, analgesic, and astringent effects on acute wounds. Nectarine leaf extract contains flavonoids, phenolic acids, terpenes, volatile oils, polysaccharides, and tannins, possessing antibacterial, anti-inflammatory, astringent, and healing-promoting effects. Compared to ordinary peach leaves, nectarine leaves have higher flavonoid and phenolic acid content and lower levels of toxic impurities such as cyanogenic glycosides, exhibiting high activity and low toxicity. It has stronger anti-inflammatory and antibacterial effects, and its moderate tannin content makes it gentle and non-irritating for astringent hemostasis. Its epidermal repair and anti-inflammatory effects are also gentler and more stable, making it more suitable for external application to wounds. Mussel adhesive protein is an acidic mucin secreted by mussels. It not only promotes hemostasis and repair but also has strong wound adhesion and film-forming properties. When added to the first composition, it can quickly form a stable, breathable, and moisture-resistant biological protective film on the wound surface during the acute phase. This compensates for the shortcomings of other components in the first composition, such as poor film formation and lack of moisture resistance. It also prevents the medication from being lost on wounds with excessive exudate and moisture, thus increasing the drug's residence time. The protective film it forms can also buffer and slow the release of Centella asiatica volatile oil and borneol, reducing stinging sensation and improving user comfort. Furthermore, the protein structure of mussel adhesive protein has a certain emulsifying and dispersing effect, which is conducive to the uniform dispersion of each component and prevents precipitation and stratification.

[0012] The second traditional Chinese medicine composition is suitable for the repair and proliferation phase. It combines extracts of red algae, astragalus, total saponins of Panax notoginseng, aloe vera, Dendrobium officinale, and Hippophae rhamnoides, which have excellent moisturizing, film-forming, angiogenesis-promoting, collagen synthesis-promoting, and epithelial regeneration effects on wounds in the proliferation phase. During the repair and proliferation phase, when there is no obvious infection or exudation, the focus shifts to gently promoting healing and granulation tissue growth. The nectarine leaf extract added at this stage is only used as an adjunct anti-inflammatory agent, and therefore the dosage is significantly lower than in the acute phase. This avoids irritating the newly formed delicate tissue with high concentrations of phenolic acids while highlighting the effects of other healing-promoting components. The purified peptide from Pinctada martensii is a small-molecule amphoteric peptide that can promote fibroblast / keratinocyte proliferation, regulate the collagen I / III ratio, and improve healing quality. Due to its small molecule size, it is absorbed quickly and has a relatively faster onset of action. Furthermore, as an amphoteric peptide, it can enhance the solubility and compatibility stability of polysaccharides, saponins, flavonoids, etc., and reduce system turbidity. Snail glycosaminoglycans are galactosylated glycosaminoglycans isolated from snail mucus. They can provide a continuously moist environment, creating a good repair microenvironment, and spontaneously form a three-dimensional network structure to provide a scaffold for repair cells, promoting angiogenesis, granulation tissue formation and collagen deposition. They can also reduce surface tension, making it easier for the drug solution to spread on the wound surface and form a uniform distribution. In addition, the addition of snail glycosaminoglycans helps to form a buffer system, reduce pH fluctuations, improve the stability of the formulation, and improve skin adaptability.

[0013] Preferably, in the first traditional Chinese medicine composition, each component, based on 100 parts by weight, comprises: 1.5–3.0 parts of *Callicarpa nudiflora* extract, 1.5–2.0 parts of *Nectaris nectarine* leaf extract, 0.8–1.5 parts of *Callicarpa nudiflora* extract, 0.2–0.5 parts of *Dracaena cochinchinensis* micro-powder, 0.5–1.2 parts of *Portulaca oleracea* extract, 0.05–0.2 parts of *Centella asiatica* volatile oil, 0.4–1.0 parts of *Rheum palmatum* extract, 0.1–0.3 parts of borneol, 0.5–1.5 parts of mussel adhesive protein, 1.5–3.0 parts of medicinal glycerin, and the remainder being purified water.

[0014] Preferably, in the second traditional Chinese medicine composition, each component, based on 100 parts by weight, comprises: 1.0–2.0 parts of red algae extract, 0.4–1.0 parts of peach leaf extract, 0.8–1.8 parts of astragalus extract, 0.3–0.8 parts of total saponins of Panax notoginseng, 0.6–1.5 parts of aloe vera extract, 0.5–1.2 parts of Dendrobium officinale extract, 0.4–1.0 parts of sea buckthorn extract, 0.1–0.4 parts of purified peptides from Pinctada martensii, 0.2–0.6 parts of snail glycosaminoglycans, 1.5–3.0 parts of medicinal glycerin, with the remainder being purified water.

[0015] As a further preferred embodiment, the components in the first traditional Chinese medicine composition, based on 100 parts by weight, are as follows: 2 parts of Naked Flower Purple Beauty Extract, 1.8 parts of Nectarine Leaf Extract, 1 part of Purple Beauty Grass Extract, 0.3 parts of Dragon's Blood Micropowder, 0.8 parts of Purslane Extract, 0.1 parts of Centella Asiatica Volatile Oil, 0.6 parts of Rhubarb Extract, 0.2 parts of Borneol, 1 part of Mussel Adhesive Protein, 2 parts of Medicinal Glycerin, and the remainder is purified water.

[0016] As a further preferred embodiment, the second traditional Chinese medicine composition comprises, by weight, the following components: 1.5 parts red algae extract, 0.6 parts peach leaf extract, 1.2 parts astragalus extract, 0.5 parts total saponins of Panax notoginseng, 1 part aloe vera extract, 0.8 parts Dendrobium officinale extract, 0.6 parts sea buckthorn extract, 0.3 parts purified peptides of Pinctada martensii, 0.4 parts snail glycosaminoglycans, 2 parts medicinal glycerin, and the remainder being purified water.

[0017] The preferred preparation method of the nectarine leaf extract is as follows: take nectarine leaves, wash them, dry them, pulverize them to 20-40 mesh, add them to a 50-75wt% ethanol aqueous solution, with a material-to-liquid ratio of 1:10-15, extract them by ultrasonic extraction at 40-60℃ for 30-60 min, filter them, concentrate the filtrate under reduced pressure, and dry them to obtain the extract.

[0018] The second part of this invention provides a method for preparing the above-mentioned traditional Chinese medicine composition.

[0019] The preparation method of the first traditional Chinese medicine composition is as follows: first, mix the extracts of naked flower purple pearl, nectarine leaf extract, purple pearl grass extract, purslane extract and rhubarb extract evenly, then add medicinal glycerin and mix evenly, then add purified water and stir until completely dissolved, then add dragon's blood powder, borneol, centella asiatica volatile oil and mussel adhesive protein in sequence, stir to disperse and let stand to defoam, and it is ready.

[0020] The preparation method of the second traditional Chinese medicine composition is as follows: First, add the red algae extract, astragalus extract, dendrobium officinale extract and sea buckthorn extract to purified water, stir and dissolve at 40~50℃, then add nectarine leaf extract, aloe extract, total saponins of Panax notoginseng and medicinal glycerin and stir evenly, then add purified peptides of Pinctada martensii and snail glycosaminoglycans and stir evenly, cool to room temperature, stand and degas, and it is ready.

[0021] The third part of this invention provides a method for applying the above-mentioned traditional Chinese medicine composition.

[0022] The first traditional Chinese medicine composition is to be used within 3 days after the wound appears. The method of use is as follows: first clean the wound with saline to remove dirt and secretions, and then spray the first traditional Chinese medicine composition evenly on the wound and surrounding skin 2 to 3 times a day.

[0023] The second traditional Chinese medicine composition is used 4 to 14 days after the wound appears. The method of use is as follows: there is no need for excessive cleaning. Just gently wipe away the secretions and spray the second traditional Chinese medicine composition evenly on the wound and surrounding skin 1 to 2 times a day.

[0024] This invention provides a traditional Chinese medicine composition for promoting wound healing and its preparation method, the outstanding features and excellent effects of which are as follows: First, the traditional Chinese medicine composition of the present invention comprises two phases of sub-compositions. The first traditional Chinese medicine composition is applied to the acute phase, and the second traditional Chinese medicine composition is applied to the repair and proliferation phase. Through the targeted formulation, different effects are produced at different stages.

[0025] Secondly, the first traditional Chinese medicine composition, based on the combined use of extracts of *Callicarpa nudiflora*, *Callicarpa japonica*, *Dracaena cochinchinensis* micron powder, *Portulaca oleracea* extract, *Centella asiatica* volatile oil, and *Rheum palmatum* extract, adds *Prunus cerasifera* leaf extract, which has a higher content of active ingredients, lower content of toxic impurities, and mild astringent and hemostatic properties. It also adds mussel adhesive protein, which can improve hemostasis, adhesion, film-forming properties, moisture resistance, and dispersibility. Under the combined action of the components of the first traditional Chinese medicine composition, it exerts good antibacterial, anti-inflammatory, hemostatic, analgesic, and astringent effects on wounds, providing a favorable environment for rapid wound repair and healing.

[0026] Third, the second traditional Chinese medicine composition, based on the combined use of red algae extract, astragalus extract, total saponins of Panax notoginseng, aloe vera extract, Dendrobium officinale extract, and sea buckthorn extract, adds a small amount of peach leaf extract to assist in anti-inflammation. It also adds purified peptides from Pinctada martensii, which are absorbed quickly, improving healing quality and speed while enhancing solubility and compatibility stability. Furthermore, it adds snail glycosaminoglycans to promote uniform spreading of the medicinal solution, reduce pH fluctuations, and create a favorable repair microenvironment, providing a scaffold for repair cells and promoting regeneration. Under the combined effect of the components of the second traditional Chinese medicine composition, it can effectively promote angiogenesis, collagen synthesis, and epithelial regeneration, achieving rapid wound repair and healing. Detailed Implementation

[0027] The following embodiments are preferred embodiments and effect verifications of the technical solutions of the present invention, and do not constitute a limitation on the scope of protection.

[0028] Example 1:

[0029] Raw material introduction: Extracts of *Callicarpa nudiflora*, *Callicarpa japonica*, *Portulaca oleracea*, rhubarb, *Astragalus membranaceus*, *Dendrobium officinale*, and *Hippophae rhamnoides* were all from Lanzhou Waterles Biotechnology Co., Ltd.; *Dracaena cochinchinensis* micron powder was from Shaanxi Mufan Biotechnology Co., Ltd.; *Centella asiatica* volatile oil was from Wuhan Jiangxin Biotechnology Co., Ltd.; and red algae extract was from Jiangsu Enming Bioengineering Technology Co., Ltd. Mussel adhesive protein was from Xi'an Shilin Biotechnology Co., Ltd.; *Pinctada martensii* purified peptides were prepared in-house, with the preparation process referring to "Purification and Identification of Immunologically Active Peptides from *Pinctada martensii*"; snail glycosaminoglycans were prepared in-house, with the preparation process referring to "Chemical Structure and Anticoagulant Activity of Snail Mucus Glycosaminoglycans". Nectarine leaf extract was prepared in-house, the preparation method being: washing and drying nectarine leaves, pulverizing to 20-40 mesh, adding to 75wt% ethanol aqueous solution at a material-to-liquid ratio of 1:10, ultrasonically extracting at 50℃ for 40 min, filtering, concentrating the filtrate under reduced pressure, and drying. All raw materials used in this embodiment and the following embodiments are from the above sources.

[0030] Preparation of the first traditional Chinese medicine composition: First, mix the extracts of *Callicarpa nudiflora*, *Prunus cerasifera* leaf, *Callicarpa japonica*, *Portulaca oleracea*, and *Rheum palmatum* evenly. Then, add medicinal glycerin and mix evenly. Next, add purified water and stir until completely dissolved. Then, add *Dragon's Blood Powder*, borneol, *Centella asiatica* volatile oil, and mussel adhesive protein in sequence. Stir to disperse and let stand to remove bubbles. The composition consists of 2 parts *Callicarpa nudiflora* extract, 1.8 parts *Prunus cerasifera* leaf extract, 1 part *Callicarpa japonica* extract, 0.3 parts *Dragon's Blood Powder*, 0.8 parts *Portulaca oleracea* extract, 0.1 parts *Centella asiatica* volatile oil, 0.6 parts *Rheum palmatum* extract, 0.2 parts borneol, 1 part mussel adhesive protein, 2 parts medicinal glycerin, and purified water to a final volume of 100 parts.

[0031] Preparation of the second traditional Chinese medicine composition: First, add red algae extract, astragalus extract, dendrobium officinale extract, and sea buckthorn extract to purified water and stir to dissolve at 45°C. Then add nectarine leaf extract, aloe extract, total saponins of Panax notoginseng, and medicinal glycerin and stir evenly. Next, add purified peptides from Pinctada martensii and snail glycosaminoglycans and stir evenly. Cool to room temperature and allow to stand to remove bubbles. The composition consists of 1.5 parts red algae extract, 0.6 parts nectarine leaf extract, 1.2 parts astragalus extract, 0.5 parts total saponins of Panax notoginseng, 1 part aloe extract, 0.8 parts dendrobium officinale extract, 0.6 parts sea buckthorn extract, 0.3 parts purified peptides from Pinctada martensii, 0.4 parts snail glycosaminoglycans, 2 parts medicinal glycerin, and purified water to a final volume of 100 parts.

[0032] Instructions for use: Use the first herbal composition within 3 days after the wound appears. First, clean the wound with saline solution, then spray the first herbal composition evenly onto the wound and surrounding skin, 3 times a day. Use the second herbal composition from the 4th to the 14th day. Gently wipe away the wound secretions, then spray the second herbal composition evenly onto the wound and surrounding skin, 2 times a day.

[0033] Example 2:

[0034] Preparation of the first traditional Chinese medicine composition: First, mix the extracts of *Callicarpa nudiflora*, *Prunus cerasifera* leaf, *Callicarpa japonica*, *Portulaca oleracea*, and *Rheum palmatum* evenly. Then, add medicinal glycerin and mix evenly. Next, add purified water and stir until completely dissolved. Then, add *Dragon's Blood* powder, borneol, *Centella asiatica* volatile oil, and mussel adhesive protein in sequence. Stir to disperse and let stand to remove bubbles. The composition includes: 2.5 parts *Callicarpa nudiflora* extract, 1.9 parts *Prunus cerasifera* leaf extract, 0.8 parts *Callicarpa japonica* extract, 0.2 parts *Dragon's Blood* powder, 0.8 parts *Portulaca oleracea* extract, 0.05 parts *Centella asiatica* volatile oil, 0.55 parts *Rheum palmatum* extract, 0.1 parts borneol, 0.6 parts mussel adhesive protein, 2 parts medicinal glycerin, and purified water to 100 parts.

[0035] Preparation of the second traditional Chinese medicine composition: First, add red algae extract, astragalus extract, dendrobium officinale extract, and sea buckthorn extract to purified water and stir to dissolve at 45°C. Then add nectarine leaf extract, aloe extract, total saponins of Panax notoginseng, and medicinal glycerin and stir evenly. Next, add purified peptides from Pinctada martensii and snail glycosaminoglycans and stir evenly. Cool to room temperature and allow to stand to remove bubbles. The composition consists of 1.1 parts red algae extract, 1.0 part nectarine leaf extract, 0.8 parts astragalus extract, 0.5 parts total saponins of Panax notoginseng, 0.6 parts aloe extract, 1.2 parts dendrobium officinale extract, 1.0 part sea buckthorn extract, 0.1 part purified peptides from Pinctada martensii, 0.6 parts snail glycosaminoglycans, 2 parts medicinal glycerin, and purified water to a final volume of 100 parts.

[0036] Usage method: Same as Example 1.

[0037] Example 3:

[0038] Preparation of the first traditional Chinese medicine composition: First, mix the extracts of *Callicarpa nudiflora*, *Prunus cerasifera* leaf, *Callicarpa japonica*, *Portulaca oleracea*, and *Rheum palmatum* evenly. Then, add medicinal glycerin and mix evenly. Next, add purified water and stir until completely dissolved. Then, add *Dragon's Blood Powder*, borneol, *Centella asiatica* volatile oil, and mussel adhesive protein in sequence. Stir to disperse and let stand to remove bubbles. The composition is as follows: 1.8 parts *Callicarpa nudiflora* extract, 1.7 parts *Prunus cerasifera* leaf extract, 0.8 parts *Callicarpa japonica* extract, 0.2 parts *Dragon's Blood Powder*, 0.9 parts *Portulaca oleracea* extract, 0.2 parts *Centella asiatica* volatile oil, 0.8 parts *Rheum palmatum* extract, 0.3 parts borneol, 0.8 parts mussel adhesive protein, 2 parts medicinal glycerin, and purified water to 100 parts.

[0039] Preparation of the second traditional Chinese medicine composition: First, add red algae extract, astragalus extract, dendrobium officinale extract, and sea buckthorn extract to purified water and stir to dissolve at 40℃. Then add nectarine leaf extract, aloe extract, total saponins of Panax notoginseng, and medicinal glycerin and stir evenly. Next, add purified peptides from Pinctada martensii and snail glycosaminoglycans and stir evenly. Cool to room temperature and allow to stand to remove bubbles. The composition consists of 1.8 parts red algae extract, 0.4 parts nectarine leaf extract, 1.8 parts astragalus extract, 0.3 parts total saponins of Panax notoginseng, 0.7 parts aloe extract, 0.7 parts dendrobium officinale extract, 0.5 parts sea buckthorn extract, 0.4 parts purified peptides from Pinctada martensii, 0.3 parts snail glycosaminoglycans, 2 parts medicinal glycerin, and purified water to a final volume of 100 parts.

[0040] Usage method: Same as Example 1.

[0041] Example 4:

[0042] Preparation of the first traditional Chinese medicine composition: First, mix the extracts of *Callicarpa nudiflora*, *Prunus cerasifera* leaf, *Callicarpa japonica*, *Portulaca oleracea*, and *Rheum palmatum* evenly. Then, add medicinal glycerin and mix evenly. Next, add purified water and stir until completely dissolved. Then, add *Dragon's Blood* powder, borneol, *Centella asiatica* volatile oil, and mussel adhesive protein in sequence. Stir to disperse and let stand to remove bubbles. The composition consists of 1.5 parts *Callicarpa nudiflora* extract, 1.5 parts *Prunus cerasifera* leaf extract, 1.2 parts *Callicarpa japonica* extract, 0.3 parts *Dragon's Blood* powder, 1.2 parts *Portulaca oleracea* extract, 0.2 parts *Centella asiatica* volatile oil, 0.6 parts *Rheum palmatum* extract, 0.2 parts borneol, 1.2 parts mussel adhesive protein, 2 parts medicinal glycerin, and purified water to a final volume of 100 parts.

[0043] Preparation of the second traditional Chinese medicine composition: First, add red algae extract, astragalus extract, dendrobium officinale extract, and sea buckthorn extract to purified water and stir to dissolve at 50°C. Then add nectarine leaf extract, aloe extract, total saponins of Panax notoginseng, and medicinal glycerin and stir evenly. Next, add purified peptides from Pinctada martensii and snail glycosaminoglycans and stir evenly. Cool to room temperature and allow to stand to remove bubbles. The composition consists of 1.2 parts red algae extract, 0.6 parts nectarine leaf extract, 1.4 parts astragalus extract, 0.5 parts total saponins of Panax notoginseng, 1.5 parts aloe extract, 0.5 parts dendrobium officinale extract, 0.5 parts sea buckthorn extract, 0.2 parts purified peptides from Pinctada martensii, 0.5 parts snail glycosaminoglycans, 2 parts medicinal glycerin, and purified water to a final volume of 100 parts.

[0044] Usage method: Same as Example 1.

[0045] Four SD rats (weighing 200-250g) were used. Their backs were shaved and disinfected. Two symmetrical full-thickness wounds were created using an 8mm punch, removing the epidermis and dermis down to the superficial fascia. Each rat was divided into a control group and an experimental group (left and right sides). The control group received physiological saline without active ingredients, while the experimental group received the traditional Chinese medicine compositions described in Examples 1-4, respectively. The application methods were as described above. Wound photographs were taken on days 7 and 14, and the area was measured using ImageJ software. The wound closure rate at 7 and 14 days was calculated as: (initial wound area - wound area at test) × 100% / initial wound area. The results are shown in Table 1.

[0046] Table 1:

Claims

1. A traditional Chinese medicine composition for promoting wound healing, characterized in that, The traditional Chinese medicine composition comprises two stages of sub-compositions, namely a first traditional Chinese medicine composition and a second traditional Chinese medicine composition; The first traditional Chinese medicine composition includes extracts of Callicarpa nudiflora, Nectarine leaf extract, Callicarpa japonica extract, Dragon's blood powder, Portulaca oleracea extract, Centella asiatica volatile oil, Rheum palmatum extract, Borneol, Mussel adhesive protein, medicinal glycerin, and purified water; The second traditional Chinese medicine composition includes red algae extract, nectarine leaf extract, astragalus extract, total saponins of Panax notoginseng, aloe extract, Dendrobium officinale extract, sea buckthorn extract, purified peptides of Pinctada martensii, snail glycosaminoglycans, pharmaceutical glycerin, and purified water.

2. The traditional Chinese medicine composition according to claim 1, characterized in that, In the first traditional Chinese medicine composition, based on 100 parts by weight, each component is as follows: 1.5-3.0 parts of Naked Flower Purple Callicarpa Extract, 1.5-2.0 parts of Nectarine Leaf Extract, 0.8-1.5 parts of Purple Callicarpa Extract, 0.2-0.5 parts of Dragon's Blood Micropowder, 0.5-1.2 parts of Purslane Extract, 0.05-0.2 parts of Centella Asiatica Volatile Oil, 0.4-1.0 parts of Rhubarb Extract, 0.1-0.3 parts of Borneol, 0.5-1.5 parts of Mussel Adhesive Protein, 1.5-3.0 parts of Medicinal Glycerin, and the balance being purified water.

3. The traditional Chinese medicine composition according to claim 1, characterized in that, The second traditional Chinese medicine composition comprises, by weight, 1.0–2.0 parts of red algae extract, 0.4–1.0 parts of peach leaf extract, 0.8–1.8 parts of astragalus extract, 0.3–0.8 parts of total saponins of Panax notoginseng, 0.6–1.5 parts of aloe vera extract, 0.5–1.2 parts of Dendrobium officinale extract, 0.4–1.0 parts of sea buckthorn extract, 0.1–0.4 parts of purified peptides from Pinctada martensii, 0.2–0.6 parts of snail glycosaminoglycans, 1.5–3.0 parts of medicinal glycerin, with the remainder being purified water.

4. The traditional Chinese medicine composition according to claim 2, characterized in that, In the first traditional Chinese medicine composition, the components, based on 100 parts by weight, are as follows: 2 parts of Naked Flower Purple Callicarpa Extract, 1.8 parts of Nectarine Leaf Extract, 1 part of Purple Callicarpa Extract, 0.3 parts of Dragon's Blood Micropowder, 0.8 parts of Purslane Extract, 0.1 parts of Centella Asiatica Volatile Oil, 0.6 parts of Rhubarb Extract, 0.2 parts of Borneol, 1 part of Mussel Adhesive Protein, 2 parts of Medicinal Glycerin, and the remainder is purified water.

5. The traditional Chinese medicine composition according to claim 3, characterized in that, The second traditional Chinese medicine composition comprises, by weight, 1.5 parts red algae extract, 0.6 parts peach leaf extract, 1.2 parts astragalus extract, 0.5 parts total saponins of Panax notoginseng, 1 part aloe extract, 0.8 parts Dendrobium officinale extract, 0.6 parts sea buckthorn extract, 0.3 parts purified peptides of Pinctada martensii, 0.4 parts snail glycosaminoglycans, 2 parts medicinal glycerin, and the remainder being purified water.

6. The traditional Chinese medicine composition according to claim 1, characterized in that, The preparation method of the nectarine leaf extract is as follows: take nectarine leaves, wash them, dry them, pulverize them to 20-40 mesh, add them to 50-75wt% ethanol aqueous solution, with a material-to-liquid ratio of 1:10-15, extract them by ultrasonic extraction at 40-60℃ for 30-60 min, filter them, concentrate the filtrate under reduced pressure, and dry them to obtain the extract.

7. A method for preparing the traditional Chinese medicine composition according to any one of claims 1 to 6, characterized in that, The preparation method of the first traditional Chinese medicine composition is as follows: first, mix the extracts of naked flower purple pearl, nectarine leaf extract, purple pearl grass extract, purslane extract and rhubarb extract evenly, then add medicinal glycerin and mix evenly, then add purified water and stir until completely dissolved, then add dragon's blood powder, borneol, centella asiatica volatile oil and mussel adhesive protein in sequence, stir to disperse and let stand to defoam, and it is ready.

8. A method for preparing the traditional Chinese medicine composition according to any one of claims 1 to 6, characterized in that, The preparation method of the second traditional Chinese medicine composition is as follows: First, add the red algae extract, astragalus extract, dendrobium officinale extract and sea buckthorn extract to purified water, stir and dissolve at 40~50℃, then add nectarine leaf extract, aloe extract, total saponins of Panax notoginseng and medicinal glycerin and stir evenly, then add purified peptides of Pinctada martensii and snail glycosaminoglycans and stir evenly, cool to room temperature, stand and degas, and it is ready.

9. The method of applying the traditional Chinese medicine composition according to any one of claims 1 to 6, characterized in that, The first traditional Chinese medicine composition is to be used within 3 days after the wound appears. The method of use is as follows: first clean the wound with saline to remove dirt and secretions, and then spray the first traditional Chinese medicine composition evenly on the wound and surrounding skin 2 to 3 times a day.

10. The method of applying the traditional Chinese medicine composition according to any one of claims 1 to 6, characterized in that, The second traditional Chinese medicine composition is used 4 to 14 days after the wound appears. The method of use is as follows: there is no need for excessive cleaning. Just gently wipe away the secretions and spray the second traditional Chinese medicine composition evenly on the wound and surrounding skin 1 to 2 times a day.

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