A porcine circovirus vaccine protein purification device

Abstract

The present application relates to the technical field of protein purification, and particularly relates to a porcine circovirus vaccine protein purification device, which comprises a protein purification instrument, a tray is arranged on the top of the protein purification instrument, and a connecting mechanism is arranged at the connecting position of the tray and the protein purification instrument. The tray is installed and fixed through the mutual insertion of the convex block and the pin rod, so that the tray is more convenient to take down and install and is easy to clean. The internal inclined surface of the tray achieves the effect of flow guiding, liquid is stored in the water collecting box, dripping on the experiment table is avoided, the safety of the experiment is improved, the height adjustment of the bracket is more convenient through the cooperation of the arc-shaped clamping block and the arc-shaped groove, the positioning effect is achieved through the cooperation of the spherical convex and the spherical groove, the adjustment of the installation position of the pre-packed column is more convenient, and the operation is more simple and convenient during the protein purification operation.

Description

Technical Field

[0001] This invention relates to the field of protein purification technology, specifically to a porcine circovirus vaccine protein purification device. Background Technology

[0002] In the production of porcine circovirus (PCV) vaccines, protein purification is a crucial step in obtaining high-purity, highly active viral antigens (such as structural proteins and virus-like particles, VLPs). After obtaining a crude extract containing viral antigens through cell culture, it must undergo multiple steps of chromatography (such as affinity chromatography, ion exchange, and gel filtration) to remove impurities such as host cell proteins, nucleic acids, and endotoxins before it can be used for vaccine formulation.

[0003] During protein purification, the purification solution is usually placed on a tray, which is prone to dripping. After the purification is completed, the tray needs to be cleaned. However, the tray is usually fixed, which makes cleaning inconvenient. In addition, the installation height and position adjustment of the pre-assembled column usually require loosening the bolts, which is quite cumbersome.

[0004] Therefore, a porcine circovirus vaccine protein purification device is proposed to solve the problems mentioned above. Summary of the Invention

[0005] To address the shortcomings of existing technologies, this invention provides a porcine circovirus vaccine protein purification device. This device solves the problems that during protein purification, the auxiliary purification solution is usually placed on a tray, which is prone to dripping. After the purification process is completed, the tray needs to be cleaned, but the tray is usually fixed, which makes cleaning inconvenient. Furthermore, adjusting the installation height and position of the pre-installed column usually requires loosening bolts, making the operation cumbersome.

[0006] To achieve the above objectives, the present invention provides the following technical solution: a protein purification instrument, wherein a tray is provided on the top of the protein purification instrument, and a connecting mechanism is provided at the connection between the tray and the protein purification instrument, the connecting mechanism including a protrusion, a bump, a groove, a fixing column, a slide, a slot, a pin, a baffle and a water collection box; The protrusion is located on the front side of the tray, the protrusion is fixedly connected to the back of the tray, the groove is opened on the top of the protein purifier, the fixing column is fixedly connected to the back of the protein purifier, the slide is slidably engaged with the outside of the fixing column, the slot is opened on the outside of the slide, the pin is fixedly connected to the top of the slide, the baffle is fixedly connected to the top of the slide, and the water collection box is placed on the top of the slide.

[0007] Preferably, one end of the pin is slidably inserted into the inside of the protrusion, and there are two slots that are connected to each other.

[0008] Preferably, the bottom of the inner cavity of the tray is sloped, the protrusion is slidably inserted into the groove, and the inner wall of the groove is in contact with the outer wall of the protrusion.

[0009] Preferably, the top of the tray is provided with a fixing rod, the outside of the fixing rod is provided with an arc-shaped groove, a bracket is slidably connected to the outside of the fixing rod, an arc-shaped locking block is fixedly connected inside the bracket, and a through groove is provided on the outside of the bracket.

[0010] Preferably, the outer wall of the arc-shaped card block fits into the inner wall of the arc-shaped groove, and the arc-shaped groove is equidistantly distributed on the outer wall of the fixing rod.

[0011] Preferably, a rotating mechanism is provided at the connection between the tray and the fixed rod. The rotating mechanism includes an anti-detachment seat, a ball groove, a fixed seat, a cavity, and a spherical protrusion. The anti-detachment seat is fixedly connected to the bottom of the fixed rod, the ball groove is opened at the top of the anti-detachment seat, the fixed seat is fixedly connected to the top of the tray, the cavity is disposed inside the fixed seat, and the spherical protrusion is fixedly connected inside the fixed seat.

[0012] Preferably, the anti-detachment seat is rotatably connected inside the fixed seat, and the inner wall of the fixed seat is in contact with the outer wall of the anti-detachment seat.

[0013] Preferably, the ball grooves are equidistantly distributed on the top of the anti-detachment seat, the spherical protrusions are equidistantly distributed on the inner wall of the fixing seat, and the inner wall of the ball grooves is in contact with the outer wall of the spherical protrusions.

[0014] Compared with the prior art, the present invention provides a porcine circovirus vaccine protein purification device, which has the following beneficial effects: 1. The tray is installed and fixed by interlocking the protrusions and pins through the sliding carriage. This design makes it easier to remove and install the tray and facilitates cleaning. The inclined surface inside the tray guides the flow of liquid, allowing it to flow into the collection box for storage and preventing it from dripping onto the experimental table, thus improving the safety of the experiment.

[0015] 2. The combination of arc-shaped blocks and arc-shaped grooves makes it easier to adjust the height of the bracket and achieve a fixed effect after adjustment. The combination of spherical protrusions and spherical grooves enables positioning and makes it easier to adjust the installation position of the pre-loaded column. This makes protein purification operations simpler and more convenient. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the structure of the present invention; Figure 2 This is a schematic diagram of the back structure of the protein purification instrument of the present invention; Figure 3 This is a schematic cross-sectional view of the tray structure of the present invention; Figure 4This is a schematic diagram of the carriage structure of the present invention; Figure 5 This is a schematic cross-sectional view of the end of the bracket of the present invention; Figure 6 This is a cross-sectional view of the fixing seat of the present invention.

[0017] In the diagram: 1. Protein purification instrument; 2. Tray; 3. Protrusion; 4. Bump; 5. Groove; 6. Fixing column; 7. Slide; 8. Slot; 9. Pin; 10. Baffle; 11. Water collection box; 12. Fixing rod; 13. Arc groove; 14. Bracket; 15. Arc-shaped locking block; 16. Through groove; 17. Anti-detachment seat; 18. Ball groove; 19. Fixing seat; 20. Cavity; 21. Spherical protrusion. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention. Example:

[0019] Please see Figure 1 - Figure 6 This embodiment of a porcine circovirus vaccine protein purification device includes a protein purifier 1. The protein purifier 1 is a prior art device, and its specific structure and working principle will not be described in detail here. The model can be: Xiaoyi protein purifier. The top of the protein purifier 1 is provided with a tray 2. The connection between the tray 2 and the protein purifier 1 is provided with a connecting mechanism. The connecting mechanism includes a protrusion 3, a protrusion 4, a groove 5, a fixing column 6, a slide 7, a slot 8, a pin 9, a baffle 10 and a water collection box 11. The protrusion 3 is located on the front side of the tray 2, the protrusion 4 is fixedly connected to the back of the tray 2, the groove 5 is opened on the top of the protein purifier 1, the fixing column 6 is fixedly connected to the back of the protein purifier 1, the slide 7 is slidably engaged with the outside of the fixing column 6, the slot 8 is opened on the outside of the slide 7, the pin 9 is fixedly connected to the top of the slide 7, the baffle 10 is fixedly connected to the top of the slide 7, and the water collection box 11 is placed on the top of the slide 7.

[0020] When in use, the liquid container needs to be placed inside the tray 2. If dripping occurs, the tray 2 needs to be cleaned after protein purification. When installing after cleaning, the protrusion 3 of the tray 2 is inserted into the groove 5. At this time, the slide 7 is straightened and slid upward so that the fixing post 6 is disengaged from the top slot 8 and inserted into another slot 8. At this time, the protrusion 4 and the pin 9 are inserted into each other to install and fix the tray 2. With this setting, it is more convenient to remove and install the tray 2 and easier to clean. The inclined surface inside the tray 2 achieves the effect of guiding the flow, so that the liquid can flow into the water collection box 11 for storage and avoid dripping onto the experimental table. After straightening the slide 7, the installation and fixing of the tray 2 is convenient and does not affect the normal installation and use of the tray 2.

[0021] Please see Figure 1 - Figure 6 One end of the pin 9 is slidably inserted into the inside of the protrusion 4. There are two slots 8, which are connected. The bottom of the inner cavity of the tray 2 is sloped. The protrusion 3 is slidably inserted into the inside of the groove 5. The inner wall of the groove 5 is in contact with the outer wall of the protrusion 3.

[0022] To facilitate adjustment of the height and orientation of the bracket 14, a fixing rod 12 is provided on the top of the tray 2. An arc-shaped groove 13 is provided on the outside of the fixing rod 12. The bracket 14 is slidably connected to the outside of the fixing rod 12. An arc-shaped locking block 15 is fixedly connected inside the bracket 14. A through groove 16 is provided on the outside of the bracket 14. A rotating mechanism is provided at the connection between the tray 2 and the fixing rod 12. The rotating mechanism includes an anti-detachment seat 17, a ball groove 18, a fixing seat 19, a cavity 20, and a spherical protrusion 21. The anti-detachment seat 17 is fixedly connected to the bottom of the fixing rod 12. The ball groove 18 is opened on the top of the anti-detachment seat 17. The fixing seat 19 is fixedly connected to the top of the tray 2. The cavity 20 is located inside the fixing seat 19. The spherical protrusion 21 is fixedly connected inside the fixing seat 19.

[0023] When the height of the bracket 14 needs to be adjusted according to the usage, simply slide the bracket 14 upwards. Through the setting of the through groove 16, the contact part between the bracket 14 and the fixing rod 12 is deformed. At this time, the arc-shaped locking block 15 disengages from one arc-shaped groove 13 and locks into the arc-shaped groove 13 at the corresponding height position, thereby adjusting the height of the bracket 14. Moreover, through the cooperation of the arc-shaped locking block 15 and the arc-shaped groove 13, the height adjustment of the bracket 14 is more convenient and can achieve the effect of fixing after adjustment. When the direction of the bracket 14 needs to be adjusted, the fixing rod 12 can be rotated, and through the setting of the cavity 20, the spherical protrusion 21 and the ball groove 18 can be rotated and engaged, thereby achieving the effect of damping the rotation of the fixing rod 12. After the fixing rod 12 rotates, the positioning effect can be achieved through the cooperation of the spherical protrusion 21 and the ball groove 18, making it more convenient to adjust the installation position of the pre-packed column and making the operation simpler and more convenient during protein purification.

[0024] Please see Figure 1 - Figure 6 The outer wall of the arc-shaped locking block 15 fits against the inner wall of the arc-shaped groove 13. The arc-shaped groove 13 is equidistantly distributed on the outer wall of the fixing rod 12. The anti-detachment seat 17 is rotatably connected inside the fixing seat 19. The inner wall of the fixing seat 19 fits against the outer wall of the anti-detachment seat 17. The ball grooves 18 are equidistantly distributed on the top of the anti-detachment seat 17. The spherical protrusions 21 are equidistantly distributed on the inner wall of the fixing seat 19. The inner wall of the ball grooves 18 fits against the outer wall of the spherical protrusions 21. The working principle of the above embodiments is as follows: When in use, the liquid container needs to be placed inside the tray 2. If dripping occurs, the tray 2 needs to be cleaned after protein purification. When installing after cleaning, the protrusion 3 of the tray 2 is inserted into the groove 5. At this time, the slide 7 is straightened and slid upward so that the fixing post 6 is disengaged from the top slot 8 and inserted into another slot 8. At this time, the protrusion 4 and the pin 9 are inserted into each other to install and fix the tray 2. With this setting, it is more convenient to remove and install the tray 2 and easier to clean. The inclined surface inside the tray 2 achieves the effect of guiding the flow, so that the liquid can flow into the water collection box 11 for storage and avoid dripping onto the experimental table. When the height of the bracket 14 needs to be adjusted according to the usage, simply slide the bracket 14 upwards. Through the setting of the through groove 16, the contact part between the bracket 14 and the fixing rod 12 is deformed. At this time, the arc-shaped locking block 15 disengages from one arc-shaped groove 13 and locks into the arc-shaped groove 13 at the corresponding height position, thereby adjusting the height of the bracket 14. Moreover, through the cooperation of the arc-shaped locking block 15 and the arc-shaped groove 13, the height adjustment of the bracket 14 is more convenient and can achieve the effect of fixing after adjustment. When the direction of the bracket 14 needs to be adjusted, the fixing rod 12 can be rotated, and through the setting of the cavity 20, the spherical protrusion 21 and the ball groove 18 can be rotated and engaged, thereby achieving the effect of damping the rotation of the fixing rod 12. After the fixing rod 12 rotates, the positioning effect can be achieved through the cooperation of the spherical protrusion 21 and the ball groove 18, making it more convenient to adjust the installation position of the pre-packed column and making the operation simpler and more convenient during protein purification.

[0025] The installation, connection, or setting methods disclosed in this embodiment are all common mechanical connection methods. As long as they can achieve their beneficial effects, they can be implemented. Therefore, this embodiment will not elaborate on their specific structural composition and working principle.

[0026] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A porcine circovirus vaccine protein purification apparatus, characterized by: The device includes a protein purification instrument (1), with a tray (2) on the top of the protein purification instrument (1). A connecting mechanism is provided at the connection between the tray (2) and the protein purification instrument (1). The connecting mechanism includes a protrusion (3), a bump (4), a groove (5), a fixing column (6), a slide (7), a slot (8), a pin (9), a baffle (10), and a water collection box (11). The protrusion (3) is located on the front side of the tray (2), the protrusion (4) is fixedly connected to the back of the tray (2), the groove (5) is opened on the top of the protein purifier (1), the fixing column (6) is fixedly connected to the back of the protein purifier (1), the slide (7) is slidably engaged with the outside of the fixing column (6), the slot (8) is opened on the outside of the slide (7), the pin (9) is fixedly connected to the top of the slide (7), the baffle (10) is fixedly connected to the top of the slide (7), and the water collection box (11) is placed on the top of the slide (7).

2. The porcine circovirus vaccine protein purification device of claim 1, wherein: One end of the pin (9) is slidably inserted into the inside of the protrusion (4), and there are two slots (8) connected to each other.

3. The porcine circovirus vaccine protein purification device of claim 2, wherein: The bottom of the inner cavity of the tray (2) is set with an incline, and the protrusion (3) is slidably inserted into the groove (5). The inner wall of the groove (5) is in contact with the outer wall of the protrusion (3).

4. The porcine circovirus vaccine protein purification device according to claim 1, characterized in that: The top of the tray (2) is provided with a fixing rod (12), the outside of the fixing rod (12) is provided with an arc groove (13), the outside of the fixing rod (12) is slidably connected with a bracket (14), the inside of the bracket (14) is fixedly connected with an arc-shaped locking block (15), and the outside of the bracket (14) is provided with a through groove (16).

5. The porcine circovirus vaccine protein purification device according to claim 4, characterized in that: The outer wall of the arc-shaped card block (15) fits against the inner wall of the arc-shaped groove (13), and the arc-shaped groove (13) is equidistantly distributed on the outer wall of the fixing rod (12).

6. The porcine circovirus vaccine protein purification device according to claim 4, characterized in that: A rotating mechanism is provided at the connection between the tray (2) and the fixed rod (12). The rotating mechanism includes an anti-detachment seat (17), a ball groove (18), a fixed seat (19), a cavity (20), and a spherical protrusion (21). The anti-detachment seat (17) is fixedly connected to the bottom of the fixed rod (12). The ball groove (18) is opened on the top of the anti-detachment seat (17). The fixed seat (19) is fixedly connected to the top of the tray (2). The cavity (20) is located inside the fixed seat (19). The spherical protrusion (21) is fixedly connected inside the fixed seat (19).

7. The porcine circovirus vaccine protein purification apparatus according to claim 6, characterized in that: The anti-detachment seat (17) is rotatably connected inside the fixed seat (19), and the inner wall of the fixed seat (19) is in contact with the outer wall of the anti-detachment seat (17).

8. The porcine circovirus vaccine protein purification apparatus according to claim 6, characterized in that: The ball grooves (18) are equidistantly distributed on the top of the anti-detachment seat (17), and the spherical protrusions (21) are equidistantly distributed on the inner wall of the fixed seat (19). The inner wall of the ball grooves (18) is in contact with the outer wall of the spherical protrusions (21).

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