A nitrogen conservation organic fertilizer containing urease inhibitor and a preparation method thereof

Abstract

The application belongs to the technical field of organic fertilizer preparation, and particularly discloses a nitrogen-preserving organic fertilizer containing urease inhibitors and a preparation method thereof. The nitrogen-preserving organic fertilizer contains the following components in parts by weight: 190 parts of livestock and poultry manure, 10 parts of organic waste auxiliary materials, and 0.1 part of urease inhibitors. The nitrogen-preserving organic fertilizer containing urease inhibitors and the preparation method thereof can strengthen nitrogen preservation in composting, and realize greenhouse gas emission reduction and crop growth improvement.

Description

Technical Field

[0001] This invention relates to the field of organic fertilizer preparation technology, and in particular to a nitrogen-retaining organic fertilizer containing a urease inhibitor and its preparation method. Background Technology

[0002] As the world's largest livestock producer, my country generates over 60 million tons of chicken manure annually. Its high nitrogen and low carbon content makes it prone to nitrogen volatilization, greenhouse gas emissions, and fertilizer quality degradation during processing. Improper disposal can exacerbate environmental pollution risks. Returning high-quality chicken manure organic fertilizer to the fields can improve soil structure, reduce reliance on chemical fertilizers, and is a key pathway to achieving the resource recycling of livestock and poultry manure. This is of great significance for achieving the goal of "achieving a comprehensive utilization rate of livestock and poultry manure of over 85% by 2030" outlined in the "Guiding Opinions on Accelerating the Comprehensive Green Transformation of Agricultural Development and Promoting Rural Ecological Revitalization."

[0003] Chicken manure, as a dominant waste product of livestock and poultry farming, is characterized by its high yield, dense texture, and high moisture content. Its high nitrogen content gives it immense potential for fertilizer use, making it a core carrier for resource reuse in the agricultural circular economy. However, traditional manure composting, especially chicken manure composting alone, results in significant nitrogen loss. Furthermore, existing organic fertilizers often suffer from inherent defects such as insufficient nutrient supply matching crop growth requirements and inadequate decomposition, making it difficult to simultaneously achieve the multi-dimensional regulatory goals of efficient nitrogen retention, synergistic greenhouse gas emission reduction, increased crop yield, and improved agricultural product quality.

[0004] In existing technologies, the composting process is optimized by adding inhibitors. The addition of urease inhibitors can suppress the slow hydrolysis of urea nitrogen, reduce the loss of ammonia nitrogen through volatilization, and decrease emissions of greenhouse gases with strong global warming potential, such as nitrous oxide. This achieves efficient retention of nitrogen nutrients, improves the nutrient concentration and quality of compost products, and reduces the risk of secondary environmental pollution during composting. However, the addition of urease inhibitors to manure for composting currently faces many technical bottlenecks and application challenges, making it difficult to achieve the dual goals of "nitrogen preservation" and "greenhouse gas emission reduction." Summary of the Invention

[0005] The purpose of this invention is to provide a nitrogen-retaining organic fertilizer containing urease inhibitors and its preparation method, which can precisely regulate urease activity, enhance nitrogen retention in compost, and meet the needs of greenhouse gas emission reduction and crop growth quality and efficiency improvement.

[0006] To achieve the above objectives, the present invention provides a nitrogen-retaining organic fertilizer containing a urease inhibitor, comprising the following components in parts by weight: 190 samples of livestock and poultry manure; 10 portions of organic waste auxiliary materials; 0.1 parts of urease inhibitor.

[0007] Preferably, the organic waste auxiliary material is one or more of the following: corn stalks, wheat stalks, rice stalks, mushroom residue, sugarcane bagasse, mushroom residue, rice husks, peanut shells, sawdust, and wood ash; The urease inhibitor is one or both of N-butylthiophosphoric triamine and N-(N-propyl)-thiophosphoric triamide.

[0008] Preferably, it includes the following components in parts by weight: 190 samples of livestock and poultry manure; 10 portions of wheat straw; 0.1 parts of N-butylthiophosphoric triamine.

[0009] Preferably, it includes the following components in parts by weight: 190 samples of livestock and poultry manure; 10 portions of wheat straw; 0.075 parts of N-butylthiophosphoric triamine; 0.025 parts of N-(N-propyl)-thiophosphate triamide.

[0010] Preferably, it includes the following components in parts by weight: 190 portions of chicken manure; 10 portions of wheat straw; 0.075 parts of N-butylthiophosphoric triamine; 0.025 parts of N-(N-propyl)-thiophosphate triamide.

[0011] This invention also provides a method for preparing a nitrogen-retaining organic fertilizer containing a urease inhibitor, comprising the following steps: S1. Raw Material Pretreatment and Mixing: Crush organic waste materials with a moisture content of 10-15%, then mix them with livestock and poultry manure to obtain organic materials. Dissolve 0.1 parts of urease inhibitor in 2L of purified water. Spray 2L of urease inhibitor solution onto every 200 parts of the mixture, mix thoroughly, and pile to a 1×1×1m³ level. 3 The pile body; S2, Anoxic composting: The compost obtained from S1 is subjected to anoxic composting for 35 days to obtain well-rotted fertilizer with a moisture content of 10-20%. S3. Air-drying and crushing: The pile obtained in S2 is air-dried and crushed to obtain powdered nitrogen-retaining organic fertilizer.

[0012] Preferably, in S1, the organic waste auxiliary material is wheat straw, which is dried to a moisture content of 10-15% and then crushed to 5-10cm; the livestock and poultry manure is chicken manure.

[0013] Preferably, in S2, during the facultative anaerobic composting fermentation process, the compost is turned over locally and samples are taken on days 0, 3, 7, 14, 21, 28, and 35.

[0014] Preferably, in S2, during the facultative anaerobic composting fermentation process, the release concentrations of ammonia, carbon dioxide, methane, and nitrous oxide are monitored.

[0015] Preferably, in S2, during the facultative anaerobic composting fermentation process, samples are taken at multiple time points within the composting cycle to analyze the product's nutrient content, humic acid content, and maturity index.

[0016] Therefore, the present invention employs the above-mentioned nitrogen-retaining organic fertilizer containing a urease inhibitor and its preparation method, and the beneficial effects are as follows: This invention uses organic waste as an auxiliary material. Its loose and porous structure optimizes the aeration and porosity of the composting system, and retains the ammonia nitrogen released during composting through physical adsorption, reducing nitrogen nutrient loss. Adding a certain amount of livestock and poultry manure, rich in organic matter and minerals such as nitrogen, phosphorus, and potassium, and carrying native functional microbial communities, provides sufficient energy and nutrient substrate for composting and initiates the organic matter degradation chain, accelerating the composting process. Using urease inhibitors, the invention directly disrupts the catalytic conformation stability of urease by targeting the urease catalytic mechanism and the compost micro-ecosystem, effectively inhibiting urease activity, delaying the conversion of urea nitrogen to ammonia nitrogen, reducing ammonia volatilization and nitrous oxide emissions, and improving the maturity and nutrient content of the compost product.

[0017] This invention can enhance nitrogen preservation in compost, ensuring a balanced and stable supply of nutrients in nitrogen-preserving organic fertilizer. It can efficiently regulate crop root growth and development, and increase agricultural yield. Compared with commercial organic fertilizer, the use of nitrogen-preserving organic fertilizer reduces CO2 emissions by 69.3%, N2O emissions by 44.2%, CH4 emissions by 49.7%, and NH3 emissions by 26.6% throughout the entire growth cycle of corn, achieving synergistic reduction of greenhouse gases and ammonia, and improving crop growth quality and efficiency.

[0018] The technical solution of the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. Attached Figure Description

[0019] Figure 1 This is a statistical result of the cumulative methane emissions from an embodiment of the nitrogen-retaining organic fertilizer containing a urease inhibitor and its preparation method of the present invention. Figure 2 This is a statistical result of the cumulative carbon dioxide emissions from an embodiment of the present invention, which describes a nitrogen-retaining organic fertilizer containing a urease inhibitor and its preparation method. Figure 3 This is a statistical result of the cumulative nitrous oxide emissions from an embodiment of the nitrogen-retaining organic fertilizer containing a urease inhibitor and its preparation method according to the present invention; Figure 4This is a statistical result of the cumulative ammonia emissions from an embodiment of the nitrogen-retaining organic fertilizer containing a urease inhibitor and its preparation method according to the present invention. Detailed Implementation

[0020] The technical solution of the present invention will be further described below with reference to the accompanying drawings and embodiments.

[0021] Unless otherwise defined, the technical or scientific terms used in this invention shall have the ordinary meaning as understood by one of ordinary skill in the art to which this invention pertains.

[0022] Example 1 A nitrogen-retaining organic fertilizer containing a urease inhibitor comprises the following components in parts by weight: 190 parts chicken manure; 10 parts wheat straw; 0.1 parts N-butylthiophosphoric triamine.

[0023] Its preparation method is as follows: S1. Raw material pretreatment and mixing: The prescribed amount of wheat straw is dried until the moisture content is 10%-15%, then crushed to 5-10 cm, and then mixed with chicken manure to obtain organic material. 0.1 parts of N-butylthiophosphoric triamine are dissolved in 2L of purified water. For every 200 parts of the mixture, 2L of urease inhibitor solution is sprayed on, mixed thoroughly, and piled to a 1×1×1m³ level. 3 The heap.

[0024] S2, Anoxic Composting: The compost pile obtained in S1 was subjected to 35 days of anoxic composting, with the temperature maintained above 55 degrees Celsius for at least 7 days during the composting period, resulting in a compost pile with a moisture content of 17.5%. During the composting cycle, the release concentrations of ammonia, carbon dioxide, methane, and nitrous oxide, as well as the pile temperature, were monitored. The pile was partially turned and samples were taken at days 0, 3, 7, 14, 21, 28, and 35 to analyze the nutrient content, maturity, and seed germination index of the samples, in order to assess the nutrient preservation and maturity of the organic fertilizer.

[0025] S3. Air-drying and crushing: The pile obtained in S2 is air-dried and crushed to obtain powdered nitrogen-retaining organic fertilizer. The total nitrogen content of the nitrogen-retaining organic fertilizer is 2.71%, the total phosphorus content is 1.52%, the total potassium content is 1.31%, the humic acid content is 12.18%, and the germination rate index is 200.60%.

[0026] Example 2 A nitrogen-retaining organic fertilizer containing a urease inhibitor comprises the following components in parts by weight: 190 parts chicken manure; 10 parts wheat straw; 0.075 parts N-butylthiophosphoric triamine; 0.025 parts N-(N-propyl)-thiophosphoric triamide.

[0027] Its preparation method is as follows: S1. Raw material pretreatment and mixing: The prescribed amount of wheat straw is dried until the moisture content is 10%-15%, then crushed to 5-10 cm, and mixed with chicken manure to obtain organic material. The prescribed amounts of N-butylthiophosphoric triamine and N-(N-propyl)-thiophosphoric triamide are dissolved in 2L of purified water. For every 200 parts of the mixture, 2L of urease inhibitor solution is sprayed on, mixed thoroughly, and piled into a 1×1×1m³ pile. 3 The heap.

[0028] S2, Anoxic Composting: The compost pile obtained in S1 was subjected to 35 days of anoxic composting, with the temperature maintained above 55 degrees Celsius for at least 7 days during the composting period, resulting in a compost pile with a moisture content of 12.3%. During the composting cycle, the release concentrations of ammonia, carbon dioxide, methane, and nitrous oxide, as well as the pile temperature, were monitored. The pile was partially turned and samples were taken at days 0, 3, 7, 14, 21, 28, and 35 to analyze the nutrient content, maturity, and seed germination index of the samples, in order to assess the nutrient preservation and maturity of the organic fertilizer.

[0029] S3. Air-drying and crushing: The pile obtained in S2 is air-dried and crushed to obtain powdered nitrogen-retaining organic fertilizer. The total nitrogen content of the nitrogen-retaining organic fertilizer is 4.61%, the total phosphorus content is 1.46%, the total potassium content is 1.25%, the humic acid content is 15.66%, and the germination rate index is 313.50%.

[0030] The application of nitrogen-retaining organic fertilizer containing urease inhibitors in corn cultivation in sandy soil in this embodiment is as follows: Of the total nitrogen fertilizer required for maize throughout its growth cycle, 50% was provided by chemical fertilizer (using 28-6-6 fertilizer) and 50% by nitrogen-retaining organic fertilizer. The chemical fertilizer and nitrogen-retaining organic fertilizer were applied to sandy soil, and maize was sown in it. The maize yield at maturity was recorded as 8812.50 kg / hm². 2 .

[0031] Comparative Example 1 A chicken manure organic fertilizer comprises the following components in parts by weight: 190 portions of chicken manure; 10 portions of wheat straw.

[0032] Its preparation method is as follows: S1. Raw material pretreatment and mixing: The prescribed amount of wheat straw is dried until the moisture content is 10%-15%, then crushed to 5-10cm. It is then mixed with chicken manure to obtain organic material. For every 200 parts of the mixture, spray with 2L of purified water, mix thoroughly, and pile into a 1×1×1m³ pile. 3 The heap.

[0033] S2, Anoxic Composting: The compost pile obtained in S1 was subjected to 35 days of anoxic composting, with the temperature maintained above 55 degrees Celsius for at least 7 days during the composting period, resulting in a compost pile with a moisture content of 11.1%. During the composting cycle, the release concentrations of ammonia, carbon dioxide, methane, and nitrous oxide, as well as the pile temperature, were monitored. The pile was partially turned and samples were taken at days 0, 3, 7, 14, 21, 28, and 35 to analyze the nutrient content, maturity, and seed germination index of the samples, in order to assess the nutrient preservation and maturity of the organic fertilizer.

[0034] S3 Air-drying and Crushing: The pile obtained in S2 is air-dried and crushed to obtain powdered chicken manure organic fertilizer. The total nitrogen content of the chicken manure organic fertilizer is 2.58%, the total phosphorus content is 1.37%, the total potassium content is 1.26%, and the germination rate index is 165.80%.

[0035] The application of chicken manure organic fertilizer in corn cultivation in sandy soil, as shown in this comparative example, is as follows: Of the total nitrogen fertilizer required for the entire growth cycle of maize, 50% was provided by chemical fertilizer (using 28-6-6 fertilizer) and 50% by chicken manure organic fertilizer. The chemical fertilizer and chicken manure organic fertilizer were applied to sandy soil, and maize was sown in it. The maize yield at maturity was recorded as 7387.50 kg / hm². 2 .

[0036] Comparative Example 2 A commercial organic fertilizer, sourced from Handan Woqi Biotechnology Co., Ltd. It contains 0.70% total nitrogen, 0.50% total phosphorus, and 0.45% total potassium.

[0037] The application of this comparative example of commercial organic fertilizer in corn cultivation in sandy soil is as follows: Of the total nitrogen fertilizer required for maize throughout its growth cycle, 50% was provided by chemical fertilizer (using 28-6-6 fertilizer) and 50% by commercial organic fertilizer. The chemical fertilizer and commercial organic fertilizer were applied to sandy soil, where maize was sown. The maize yield at maturity was recorded as 6487.50 kg / hm². 2 .

[0038] Test 1. In the monitoring of the nitrogen-retaining organic fertilizer facultative anaerobic composting fermentation process in Examples 1-2 and Comparative Example 1, the compost temperature was measured using the thermistor method, ammonia was measured using the boric acid absorption-dilute sulfuric acid titration method, carbon dioxide, methane, and nitrous oxide were measured using a biogas analyzer, ammonium nitrogen and nitrate nitrogen were measured using a flow analyzer, the seed germination index was determined by culturing radish seeds at 25±1℃ for 48 hours, and total nitrogen, total phosphorus, and total potassium were measured using a concentrated sulfuric acid digestion-Kjeldahl nitrogen analyzer, a sodium hydroxide fusion-spectrophotometer, and a sodium hydroxide fusion-flame photometer, respectively.

[0039] (1) The test results of the technical indicators of maturity NY525-2021 and the examples and comparative examples are shown in Table 1.

[0040] Table 1 Technical Indicators for Decomposition Maturity

[0041] As shown in Table 1, Comparative Example 1, Example 1, and Example 2 all meet the NY525-2021 standard for organic fertilizer. The total nutrient content of Comparative Example 1, Example 1, and Example 2 exceeds the NY525-2021 standard for organic fertilizer by 30.0%, 37.5%, and 82.5%, respectively; the seed germination index of Comparative Example 1, Example 1, and Example 2 exceeds the NY525-2021 standard for organic fertilizer by 136.9%, 186.6%, and 347.9%, respectively.

[0042] (2) The test results of cumulative methane emissions are as follows: Figure 1 As shown.

[0043] Depend on Figure 1 It can be seen that there are obvious differences between treatments in the control of methane (CH4) emissions. Example 1 showed excellent CH4 emission reduction effect, with a reduction of up to 57.4% compared with Comparative Example 1. However, the CH4 emission of Example 2 did not achieve emission reduction, but instead increased slightly compared with Comparative Example 1 CK treatment, with an increase of 4.26%. This reflects the functional characteristics of different urease inhibitors in the control of greenhouse gas emissions.

[0044] (3) The test results of cumulative carbon dioxide emissions are as follows: Figure 2 As shown.

[0045] Depend on Figure 2 As can be seen, Example 2 shows a significant advantage in carbon dioxide emission reduction, with a reduction of 22.6% compared to Comparative Example 1 and a reduction of 27.3% compared to Example 1.

[0046] (4) The test results of cumulative nitrous oxide emissions are as follows: Figure 3 As shown.

[0047] Depend on Figure 3 It can be seen that the cumulative nitrous oxide emissions in the example were the lowest (40.5 μg·kg⁻¹). -1 The DM emission reduction was 5.8% compared to Comparative Example 1 and 13.9% compared to Example 1.

[0048] (5) The test results of the cumulative ammonia emissions are as follows: Figure 4 As shown.

[0049] Depend on Figure 4 It can be seen that the cumulative NH3 emissions of the nitrogen-retaining organic fertilizer treated in Example 2 were reduced by 29.3% and 37.4% compared with Comparative Example 1 and Example 1, respectively.

[0050] 2. The heavy metal and salt content of organic fertilizers in Examples 1-2 and Comparative Example 1 were tested, and the test results are shown in Table 2.

[0051] Table 2 Statistical results of heavy metal and salt content

[0052] 3. Greenhouse gas (CO2, N2O, CH4) and ammonia (NH3) emissions were monitored throughout the entire growth cycle of maize in Example 2 and Comparative Example 2. The results are shown in Table 3.

[0053] Table 3. Statistical Results of Greenhouse Gas and Ammonia Emissions

[0054] As shown in Table 3, under the premise of replacing 50% of chemical fertilizer nitrogen with organic fertilizer, the nitrogen loss in gaseous form is reduced by the nitrogen retention effect of urease inhibitor, thereby improving the nitrogen utilization rate of organic fertilizer. This ensures that nitrogen is not lost after 50% replacement of chemical fertilizer nitrogen and that the nitrogen supply to corn is sufficient. It also achieves the reduction of greenhouse gas and ammonia volatilization emissions. Compared with conventional commercial organic fertilizer, it has outstanding application advantages in reducing chemical fertilizer application, nitrogen retention, agricultural non-point source pollution control, and agricultural carbon neutrality.

[0055] Therefore, the present invention employs the above-mentioned nitrogen-retaining organic fertilizer containing urease inhibitor and its preparation method, which can precisely regulate urease activity, enhance nitrogen retention in compost, and meet the needs of greenhouse gas emission reduction and crop growth quality improvement and efficiency enhancement.

[0056] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and not to limit them. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can still be made to the technical solutions of the present invention, and these modifications or equivalent substitutions cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A nitrogen-retaining organic fertilizer containing a urease inhibitor, characterized in that, Includes the following ingredients by weight: 190 samples of livestock and poultry manure; 10 portions of organic waste auxiliary materials; 0.1 parts of urease inhibitor.

2. The nitrogen-retaining organic fertilizer containing a urease inhibitor according to claim 1, characterized in that: The organic waste auxiliary materials are one or more of the following: corn stalks, wheat stalks, rice stalks, mushroom residue, sugarcane bagasse, mushroom residue, rice husks, peanut shells, sawdust, and wood ash. The urease inhibitor is one or both of N-butylthiophosphoric triamine and N-(N-propyl)-thiophosphoric triamide.

3. The nitrogen-retaining organic fertilizer containing a urease inhibitor according to claim 2, characterized in that, Includes the following ingredients by weight: 190 samples of livestock and poultry manure; 10 portions of wheat straw; 0.1 parts of N-butylthiophosphoric triamine.

4. The nitrogen-retaining organic fertilizer containing a urease inhibitor according to claim 2, characterized in that, Includes the following ingredients by weight: 190 samples of livestock and poultry manure; 10 portions of wheat straw; 0.075 parts of N-butylthiophosphoric triamine; 0.025 parts of N-(N-propyl)-thiophosphate triamide.

5. A nitrogen-retaining organic fertilizer containing a urease inhibitor according to claim 4, characterized in that, Includes the following ingredients by weight: 190 portions of chicken manure; 10 portions of wheat straw; 0.075 parts of N-butylthiophosphoric triamine; 0.025 parts of N-(N-propyl)-thiophosphate triamide.

6. A method for preparing a nitrogen-retaining organic fertilizer containing a urease inhibitor, characterized in that, Includes the following steps: S1. Raw material pretreatment and mixing: Crush organic waste materials with a moisture content of 10-15%, then mix them with livestock and poultry manure to obtain organic materials. Dissolve 0.1 parts of urease inhibitor in 2L of purified water. Spray 2L of urease inhibitor solution on every 200 parts of the mixture, mix thoroughly, and pile into a 1×1×1m³ pile. 3 The pile body; S2, Anoxic composting: The compost obtained from S1 is subjected to anoxic composting for 35 days to obtain well-rotted fertilizer with a moisture content of 10-20%. S3. Air-drying and crushing: The pile obtained in S2 is air-dried and crushed to obtain powdered nitrogen-retaining organic fertilizer.

7. The method for preparing a nitrogen-retaining organic fertilizer containing a urease inhibitor according to claim 6, characterized in that, In S1, wheat straw is used as an auxiliary material for organic waste. The wheat straw is dried to a moisture content of 10-15% and then crushed to 5-10cm. Chicken manure is used as livestock and poultry manure.

8. The method for preparing a nitrogen-retaining organic fertilizer containing a urease inhibitor according to claim 6, characterized in that, In S2, during the facultative anaerobic composting fermentation process, the compost was partially turned over and samples were taken on days 0, 3, 7, 14, 21, 28, and 35.

9. The method for preparing a nitrogen-retaining organic fertilizer containing a urease inhibitor according to claim 6, characterized in that, In S2, during the facultative anaerobic composting fermentation process, the release concentrations of ammonia, carbon dioxide, methane, and nitrous oxide are monitored.

10. The method for preparing a nitrogen-retaining organic fertilizer containing a urease inhibitor according to claim 6, characterized in that, In S2, during the facultative anaerobic composting fermentation process, samples were taken at multiple time points within the composting cycle to analyze the product's nutrient content, humic acid content, and maturity index.

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