A multi-zone temperature control biological reaction equipment for improving the synthesis transmission efficiency of phytosterol esterase

Abstract

The application relates to a multi-zone temperature control biological reaction equipment for improving phytosterol ester synthesis transmission efficiency, and relates to the technical field of synthesis equipment, which comprises a reaction bucket, two partition plates are arranged on the inner wall of the reaction bucket, the reaction bucket is sequentially divided into a first reaction cavity, a second reaction cavity and a third reaction cavity by the two partition plates, a first motor is fixedly installed at the top of the reaction bucket, and a central shaft is fixedly connected to the output end of the first motor. The self-rotation movement of the plurality of overturning blades fixedly connected to the bevel gear side is driven, the precipitate after scraping is driven to be lifted upwards, the phytosterol ester precipitate is beneficial to being brought to the central position of the third reaction cavity, mixing with liquid phytosterol ester is realized, the lifted phytosterol ester precipitate is gradually restored to liquid state, the phytosterol ester precipitation is reduced, the yield and the yield of phytosterol ester synthesis are improved, and the synthesis transmission is facilitated.

Description

Technical Field

[0001] This application relates to the field of synthetic equipment technology, and in particular to a multi-zone temperature-controlled bioreactor that improves the transport efficiency of phytosterol ester enzyme synthesis. Background Technology

[0002] Phytosterols are a class of natural compounds with a cyclopentane-polyhydrophenanthrene backbone, widely found in plant-based foods such as corn oil. In current industrial applications, phytosterols and fatty acids are used to produce a mixture of phytosterol esters and water under the action of enzymes. The final product of phytosterol esters is a solid or a high-viscosity liquid at room temperature, while the production process involves a liquid reaction mixture that needs to be transported and processed.

[0003] In the existing synthesis process of phytosterol esters, due to the contact temperature, precipitates are generated and accumulate at the bottom of the reaction vessel, which is not conducive to the stirring and mixing with liquid phytosterol esters during the reaction process and reduces the transfer efficiency of phytosterol esters. Summary of the Invention

[0004] To address the issue that when large areas of water and oil stains are unavoidable, continued movement can cause wheel slippage, affecting the stability of the inspection device, this application provides a multi-zone temperature-controlled bioreactor that improves the transport efficiency of plant sterol ester enzyme synthesis.

[0005] This application provides a multi-zone temperature-controlled bioreactor for improving the transport efficiency of plant sterol esterase synthesis, which adopts the following technical solution: A multi-zone temperature-controlled bioreactor for improving the transport efficiency of phytosterol ester enzymatic synthesis includes a reaction tank. Two partitions are provided on the inner wall of the reaction tank, dividing the interior into a first reaction chamber, a second reaction chamber, and a third reaction chamber. A first motor is fixedly installed on the top of the reaction tank, and a central shaft is fixedly connected to the output end of the first motor. A feed inlet is provided on the top of the reaction tank, and a discharge outlet is provided on the outer wall of the reaction tank. The feed inlet is located above the first reaction chamber. The central shaft is rotatably fitted onto the inner wall of the reaction tank.

[0006] Two fixed shafts are fixedly connected to the circumferential side of the central shaft. Both fixed shafts are rotatably fitted with conical teeth. Several flipping blades are fixedly connected to one side of the conical teeth. The several flipping blades are arranged in a circumferential array on the circumferential side of the fixed shaft. The several flipping blades and the fixed shafts are rotatably fitted together.

[0007] The bottom of the third reaction chamber is fixedly equipped with a main bevel tooth, which meshes with two secondary bevel teeth.

[0008] By adopting the above technical solution, two conical teeth are driven to rotate around the central axis, which in turn drives several rotating blades fixedly connected to the sides of the two conical teeth to scrape off the solid or high-viscosity liquid phytosterol ester precipitates generated at the bottom of the reaction tank. Furthermore, through the simultaneous meshing and engagement of the two conical teeth with the main conical teeth, the several rotating blades fixedly connected to the sides of the two conical teeth are driven to rotate, thereby lifting the scraped precipitates upward.

[0009] Optionally, a central groove is provided at the top center of the main bevel tooth, and the bottom of the central shaft is rotatably engaged with the central groove.

[0010] By adopting the above technical solution, the rotational fit between the central shaft and the central groove is achieved, thus preventing the central shaft from deflecting.

[0011] Optionally, a limiting groove is provided on the inner wall of the reaction tank, and the ends of the two fixed shafts are slidably fitted on the inner wall of the limiting groove.

[0012] By adopting the above technical solution, two sliding fits are achieved on the inner wall of the limiting groove, which avoids the deflection of each fixed shaft of the chain when the fixed shaft rotates along the center of the reaction tank.

[0013] Optionally, several sets of stirring shafts are fixedly connected to the circumferential side of the central shaft, with a certain number of stirring shafts in each set, and each set of stirring shafts is respectively arranged in the first reaction chamber, the second reaction chamber and the third reaction chamber.

[0014] By adopting the above technical solution, several sets of stirring shafts arranged on the circumferential side of the central shaft can uniformly stir the reactants in the first reaction chamber, the second reaction chamber and the third reaction chamber, thereby improving the reaction efficiency.

[0015] Optionally, the reaction vessel is provided with a first temperature control coil, a second temperature control coil and a third temperature control coil in sequence from top to bottom around its circumference. The first temperature control coil is located outside the first reaction chamber, the second temperature control coil is located outside the second reaction chamber, and the third temperature control coil is located outside the third reaction chamber.

[0016] By adopting the above technical solution, multiple temperature control zones are set up in the reaction tank, namely the first reaction chamber, the second reaction chamber and the third reaction chamber, so that the reaction raw materials pass through different temperature environments in sequence during the flow process, thereby better matching the reaction requirements of each stage. Without adding additional reaction equipment, the synthesis efficiency of plant sterol ester enzyme is improved and enzyme inactivation caused by unsuitable temperature is reduced.

[0017] Optionally, the outer wall of the reaction vessel is provided with a first input three-way valve, a second input three-way valve and a third input three-way valve in sequence from top to bottom. The first input three-way valve is connected to the inlet end of the first temperature control coil, the second input three-way valve is connected to the inlet end of the second temperature control coil, and the third input three-way valve is connected to the inlet end of the third temperature control coil.

[0018] By adopting the above technical solution, the inlet ends of the first, second, and third temperature control coils are connected to the first, second, and third input three-way valves, respectively, to realize the input of the flowing medium to the three temperature control coils.

[0019] Optionally, the outer wall of the reaction vessel is provided with a first output three-way valve, a second output three-way valve and a third output three-way valve in sequence from top to bottom. The first output three-way valve is connected to the output end of the first temperature control coil, the second output three-way valve is connected to the output end of the second temperature control coil, and the third output three-way valve is connected to the output end of the third temperature control coil.

[0020] By adopting the above technical solution, the first temperature control coil output end, the second temperature control coil output end, and the third temperature control coil are respectively connected to the first output three-way valve, the second output three-way valve, and the third output three-way valve, so as to realize the output of the flowing medium of the three temperature control coils.

[0021] Optionally, the first input three-way valve, the second input three-way valve, and the third input three-way valve are respectively connected to the output ends of the externally installed cold medium tank and hot medium tank, and the first output three-way valve, the second output three-way valve, and the third output three-way valve are respectively connected to the input ends of the externally installed cold medium tank and hot medium tank.

[0022] By adopting the above technical solution, the first input three-way valve, the second input three-way valve, and the third input three-way valve are respectively connected to the output ends of the externally installed cold medium tank and hot medium tank, and the first output three-way valve, the second output three-way valve, and the third output three-way valve are respectively connected to the input ends of the externally installed cold medium tank and hot medium tank, thereby realizing the supply and transmission of hot and cold media.

[0023] Optionally, the outer wall of the reaction tank is provided with a first transmission pipe and a second transmission pipe from top to bottom. The two ends of the first transmission pipe are respectively connected to the first reaction chamber and the second reaction chamber, and the two ends of the second transmission pipe are respectively connected to the second reaction chamber and the third reaction chamber.

[0024] A second motor is fixedly installed on the outer wall of both the first and second transmission pipes. A rotating shaft is fixedly connected to the output end of each of the two second motors. Spiral auger blades are fixedly connected to the circumferential side of each of the two rotating shafts. The two spiral auger blades are respectively attached to the inner wall of the first and second transmission pipes.

[0025] By adopting the above technical solution, the spiral auger blades fixedly connected to the two rotating shafts rotate. The two rotating spiral auger blades scrape the inner walls of the first and second transmission tubes respectively, avoiding the condensation of phytosterol esters on the inner wall of the tube due to the coldness of the tube wall. At the same time, the reactants and the synthesized products are transported downward along the tube wall.

[0026] In summary, this application includes at least one of the following beneficial technical effects of a multi-zone temperature-controlled bioreactor that improves the transport efficiency of phytosterol esterase synthesis: In this application, the rotation of the central shaft drives several rotating blades to scrape off the solid or high-viscosity liquid phytosterol ester precipitates generated at the bottom of the reaction tank. Furthermore, the simultaneous meshing of two conical teeth with the main conical teeth causes several rotating blades fixedly connected to the sides of the two conical teeth to rotate, thus lifting the scraped precipitates upwards. This facilitates the transport of the phytosterol ester precipitates to the center of the third reaction chamber, where they are stirred and mixed with the liquid phytosterol esters. This allows the lifted phytosterol ester precipitates to gradually return to a liquid state, reducing phytosterol ester precipitation, increasing the yield and productivity of the enzymatic synthesis of phytosterol esters, and facilitating the transport of the synthesized product.

[0027] In this application, the reaction vessel is equipped with multiple temperature control zones, namely the first reaction chamber, the second reaction chamber, and the third reaction chamber, so that the reaction raw materials pass through different temperature environments in sequence during the flow process, thereby better matching the reaction requirements of each stage. Without adding additional reaction equipment, the synthesis efficiency of plant sterol ester-specific enzymes is improved, and enzyme inactivation caused by unsuitable temperature is reduced.

[0028] In this application, the arrangement of two spiral auger blades enables the scraping of the inner walls of the first and second transfer tubes, preventing phytosterol esters from condensing on the inner walls due to the coldness of the tubes, while simultaneously enabling the reaction products and synthesized products to be transported downwards along the tube walls. Attached Figure Description

[0029] Figure 1 This is a schematic diagram of the structure of a multi-zone temperature-controlled bioreactor for improving the transport efficiency of plant sterol esterase synthesis according to this application; Figure 2 for Figure 1 Enlarged diagram of A in the middle; Figure 3 A cross-sectional view of a multi-zone temperature-controlled bioreactor for improving the transport efficiency of plant sterol esterase synthesis; Figure 4 for Figure 3 Enlarged diagram of B in the diagram; Figure 5 for Figure 3 Enlarged diagram of C in the middle; Figure 6 for Figure 3 Enlarged diagram of D in the middle; Figure 7 A schematic diagram of the structure with the central axis; Figure 8 for Figure 7 An enlarged diagram of E in the diagram.

[0030] Explanation of reference numerals in the attached drawings: 1. Reaction tank; 101. Divider plate; 102. First reaction chamber; 103. Second reaction chamber; 104. Third reaction chamber; 105. First motor; 106. Central shaft; 1061. Stirring shaft; 107. Feed inlet; 108. Fixed shaft; 1081. Lower conical teeth; 1082. Tilting blade; 109. Main conical teeth; 1091. Central groove; 110. Limiting groove; 111. First temperature control coil; 11 2. Second temperature control coil; 113. Third temperature control coil; 114. First input three-way valve; 115. Second input three-way valve; 116. Third input three-way valve; 117. First output three-way valve; 118. Second output three-way valve; 119. Third output three-way valve; 120. First transmission pipe; 121. Second transmission pipe; 122. Second motor; 1221. Rotating shaft; 1222. Spiral auger blades; 123. Discharge port. Detailed Implementation

[0031] The technical solutions of this application will be clearly and completely described below with reference to the accompanying drawings of the embodiments. Obviously, what is described is only a part of this application, not all of it. Based on this application, all other innovations obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0032] This application discloses a multi-zone temperature-controlled bioreactor device for improving the transport efficiency of phytosterol esterase synthesis.

[0033] Example 1:

[0034] Reference Figure 3 A multi-zone temperature-controlled bioreactor for improving the transport efficiency of plant sterol ester enzyme synthesis includes a reaction tank 1. The inner wall of the reaction tank 1 is provided with two partition plates 101, which divide the interior of the reaction tank 1 into a first reaction chamber 102, a second reaction chamber 103, and a third reaction chamber 104 in sequence. A first motor 105 is fixedly installed on the top of the reaction tank 1. The output end of the first motor 105 is fixedly connected to a central shaft 106. A feed inlet 107 is provided on the top of the reaction tank 1, and a discharge outlet 123 is provided on the outer wall of the reaction tank 1. The feed inlet 107 is located above the first reaction chamber 102, and the central shaft 106 is rotatably fitted on the inner wall of the reaction tank 1.

[0035] Reference Figure 4 and Figure 8 Two fixed shafts 108 are fixedly connected to the circumference of the central shaft 106. Both fixed shafts 108 are rotatably fitted with bevel teeth 1081. Several rotating blades 1082 are fixedly connected to one side of the bevel teeth 1081. The rotating blades 1082 are arranged in a circular array on the circumference of the fixed shaft 108. The rotating blades 1082 and the fixed shaft 108 are rotatably fitted together.

[0036] Reference Figure 4 The bottom of the third reaction chamber 104 is fixedly installed with a main bevel tooth 109, which meshes with two secondary bevel teeth 1081.

[0037] Reference Figure 4 The top center of the main bevel tooth 109 has a central groove 1091, and the bottom of the central shaft 106 is rotatably engaged with the central groove 1091.

[0038] Reference Figure 6 A limiting groove 110 is provided on the inner wall of the reaction tank 1, and the ends of the two fixed shafts 108 are slidably fitted on the inner wall of the limiting groove 110.

[0039] The implementation principle of Example 1 is as follows: The interior of the reaction tank 1 is divided into three cavities by two partition plates 101. The three cavities inside the reaction tank 1 from top to bottom are the first reaction cavity 102, the second reaction cavity 103 and the third reaction cavity 104. The central shaft 106 set inside the reaction tank 1 passes through the first reaction cavity 102, the second reaction cavity 103 and the third reaction cavity 104 in sequence. The central shaft 106 is rotatably fitted at the center position of the inner wall of the reaction tank 1.

[0040] When using reaction tank 1, reaction raw materials are added to the first reaction chamber 102 of reaction tank 1 through the feed inlet 107 provided at the top of the outer side of reaction tank 1. Then the reaction raw materials pass through the first reaction chamber 102 and the second reaction chamber 103 in sequence and enter the third reaction chamber 104. Finally, the synthesized phytosterol ester mixture is discharged outward through the discharge outlet 123 provided on the outer wall of reaction tank 1 on one side of the third reaction chamber 104.

[0041] The synthesis of phytosterol esters involves using phytosterols and fatty acids as reactants, followed by high-temperature heating, to produce a mixture of phytosterol esters and water. Phytosterol esters are solids or high-viscosity liquids at room temperature.

[0042] The first motor 105, fixedly mounted on the top of the reaction vessel 1, is activated. The first motor 105 is a geared asynchronous motor, driving the central shaft 106 fixedly connected to its output end to rotate. When the central shaft 106 rotates, it drives two fixed shafts 108 fixedly connected to its circumferential side to rotate around the central shaft 106. Simultaneously, both fixed shafts 108 have bevel gears 1081 rotating around their circumferential sides. As the two fixed shafts 108 rotate around the central shaft 106, they also drive the two bevel gears 1081 to rotate around the central shaft 106. Since both bevel gears 1081 are located at the bottom of the reaction vessel 1, which is fixedly connected to the main bevel gear 1... Above 09, and with both secondary bevel teeth 1081 simultaneously meshing with the main bevel tooth 109, when the two secondary bevel teeth 1081 rotate around the central axis 106, they drive the two secondary bevel teeth 1081 to rotate on their own axis. The rotation of the two secondary bevel teeth 1081 drives the several rotating blades 1082 fixedly connected to the sides of the two secondary bevel teeth 1081 to rotate on their own axis. During the rotation and rotation of the several rotating blades 1082, their edges can fit against the bottom of the reaction vessel 1. During the rotation of the central axis 106, the bottom of the central axis 106 is always in rotational engagement with the central groove 1091 opened at the top of the main bevel tooth 109. The rotational engagement of the two prevents the central axis 106 from deflecting.

[0043] When the central shaft 106 rotates, it drives two conical teeth 1081 to rotate around the central shaft 106. This causes several rotating blades 1082, which are fixedly connected to the sides of the two conical teeth 1081, to scrape off the solid or high-viscosity liquid phytosterol ester precipitates generated at the bottom of the reaction tank 1. Furthermore, through the simultaneous meshing of the two conical teeth 1081 with the main conical teeth 109, the rotating blades 1082, which are fixedly connected to the sides of the two conical teeth 1081, rotate on their own axis. This causes the scraped precipitates to be lifted upwards, which helps the phytosterol ester precipitates to be carried to the center of the third reaction chamber 104. This allows the lifted phytosterol ester precipitates to gradually return to a liquid state, reducing the precipitation of phytosterol esters, improving the synthesis effect of the phytosterol ester-specific enzyme, and facilitating the transport of the synthesized product.

[0044] Example 2:

[0045] Reference Figure 3 Several sets of stirring shafts 1061 are fixedly connected to the circumferential side of the central shaft 106. Each set of stirring shafts 1061 has a certain number of shafts and is respectively set in the first reaction chamber 102, the second reaction chamber 103 and the third reaction chamber 104.

[0046] Reference Figure 3The reaction vessel 1 is provided with a first temperature control coil 111, a second temperature control coil 112 and a third temperature control coil 113 in sequence from top to bottom. The first temperature control coil 111 is located outside the first reaction chamber 102, the second temperature control coil 112 is located outside the second reaction chamber 103, and the third temperature control coil 113 is located outside the third reaction chamber 104.

[0047] Reference Figure 1 and Figure 3 The outer wall of the reaction vessel 1 is provided with a first input three-way valve 114, a second input three-way valve 115 and a third input three-way valve 116 from top to bottom. The first input three-way valve 114 is connected to the inlet end of the first temperature control coil 111, the second input three-way valve 115 is connected to the inlet end of the second temperature control coil 112, and the third input three-way valve 116 is connected to the inlet end of the third temperature control coil 113.

[0048] Reference Figure 1 and Figure 3 The outer wall of the reaction vessel 1 is provided with a first output three-way valve 117, a second output three-way valve 118 and a third output three-way valve 119 from top to bottom. The first output three-way valve 117 is connected to the output end of the first temperature control coil 111, the second output three-way valve 118 is connected to the output end of the second temperature control coil 112, and the third output three-way valve 119 is connected to the output end of the third temperature control coil 113.

[0049] Reference Figure 1 and Figure 3 The first input three-way valve 114, the second input three-way valve 115 and the third input three-way valve 116 are respectively connected to the output ends of the externally installed cold medium tank and hot medium tank, and the first output three-way valve 117, the second output three-way valve 118 and the third output three-way valve 119 are respectively connected to the input ends of the externally installed cold medium tank and hot medium tank.

[0050] The implementation principle of Example 2 is as follows: The reaction tank 1 is divided into a first reaction chamber 102, a second reaction chamber 103, and a third reaction chamber 104. A central shaft 106, which is rotatably fitted inside the reaction tank 1, passes through the first reaction chamber 102, the second reaction chamber 103, and the third reaction chamber 104 in sequence. The functions of the three reaction chambers from top to bottom are preheating, optimal reaction, and product stabilization, respectively. When the central shaft 106 rotates, the reaction raw materials in the three reaction chambers are stirred and mixed by a number of stirring shafts 1061 arranged on the circumferential side of the central shaft 106. There are three sets of stirring shafts 1061 in total, with a number of stirring shafts 1061 in each set, and one set of stirring shafts 1061 is arranged in each reaction chamber.

[0051] The reaction vessel 1 adopts zoned temperature control. Specifically, a first temperature control coil 111, a second temperature control coil 112, and a third temperature control coil 113 are respectively installed around the reaction vessel 1, outside the first reaction chamber 102, the second reaction chamber 103, and the third reaction chamber 104. The temperature control coil corresponding to the outside of each chamber can provide heating for the reaction raw materials inside the chamber, promoting the synthesis of plant sterol ester-specific enzymes.

[0052] The first temperature control coil 111, the second temperature control coil 112, and the third temperature control coil 113 all contain a flowing medium. The input and output ends of the first temperature control coil 111 are connected to the first input three-way valve 114 and the first output three-way valve 117, respectively. The second temperature control coil 112 is connected to the second input three-way valve 115 and the second output three-way valve 118, respectively. The input and output ends of the third temperature control coil 113 are connected to the third input three-way valve 116 and the third output three-way valve 119, respectively. The first input three-way valve 114, the second input three-way valve 115, and the third input three-way valve 116 are connected to external... The output ends of the externally installed cold medium tank and hot medium tank are connected. The first output three-way valve 117, the second output three-way valve 118, and the third output three-way valve 119 are respectively connected to the input ends of the externally installed cold medium tank and hot medium tank. Neither the cold medium tank nor the hot medium tank is shown in the figure. The medium stored in the cold medium tank is usually low-temperature heat transfer oil, cooling water, brine such as ethylene glycol aqueous solution, or industrial refrigerant. The medium stored in the hot medium tank is usually high-temperature heat transfer oil, molten salt, high-temperature steam, or high-pressure hot water. The medium stored in the cold medium tank is cooled by heat dissipation from the radiator, and the medium in the hot medium tank is heated by electric heating.

[0053] In the above process of synthesizing phytosterol esters, multiple temperature control zones are set in the reaction tank 1, namely the first reaction chamber 102, the second reaction chamber 103 and the third reaction chamber 104. The reaction raw materials pass through different temperature environments in sequence during the flow process, thereby better matching the reaction requirements of each stage. Without adding additional reaction equipment, the synthesis efficiency of phytosterol ester-specific enzymes is improved and enzyme inactivation caused by unsuitable temperature is reduced.

[0054] Example 3:

[0055] Reference Figure 3 and Figure 5 The outer wall of the reaction tank 1 is provided with a first transmission pipe 120 and a second transmission pipe 121 from top to bottom. The two ends of the first transmission pipe 120 are respectively connected to the first reaction chamber 102 and the second reaction chamber 103. The two ends of the second transmission pipe 121 are respectively connected to the second reaction chamber 103 and the third reaction chamber 104.

[0056] Reference Figure 3 and Figure 5A second motor 122 is fixedly installed on the outer wall of both the first transmission pipe 120 and the second transmission pipe 121. The output ends of the two second motors 122 are fixedly connected to a rotating shaft 1221. Spiral auger blades 1222 are fixedly connected to the circumferential sides of the two rotating shafts 1221. The two spiral auger blades 1222 are respectively attached to the inner wall of the first transmission pipe 120 and the second transmission pipe 121.

[0057] The implementation principle of Example 3 is as follows: During the reaction of the reaction tank 1 to synthesize the special enzyme for phytosterol esters, the second motor 122, which is fixedly installed on the outer wall of the first transfer tube 120 and the second transfer tube 121, is started. The second motor 122 is a geared asynchronous motor, which drives the rotating shaft 1221, which is fixedly connected to the output end of the two second motors 122, to rotate. When the two rotating shafts 1221 rotate, they drive the spiral auger blades 1222, which are fixedly connected to the circumference of the two rotating shafts 1221, to rotate. The two rotating spiral auger blades 1222 respectively scrape the inner wall of the first transfer tube 120 and the second transfer tube 121, thus preventing the phytosterol esters from condensing on the inner wall of the tube due to the coldness of the tube wall. At the same time, it realizes the downward transfer of reactants and synthesized products along the tube wall, that is, through the first transfer tube 120 from the first reaction chamber 102 to the second reaction chamber 103, and through the second transfer tube 121 from the second reaction chamber 103 to the third reaction chamber 104.

[0058] The above are all preferred embodiments of this application and are not intended to limit the scope of protection of this application. Therefore, all equivalent changes made in accordance with the structure, shape and principle of this application should be covered within the scope of protection of this application.

Claims

1. A multi-zone temperature-controlled bioreactor for improving the transport efficiency of plant sterol ester enzymatic synthesis, characterized in that: The reaction vessel includes a reaction tank (1), the inner wall of which is provided with two partition plates (101), which divide the interior of the reaction tank (1) into a first reaction chamber (102), a second reaction chamber (103) and a third reaction chamber (104) in sequence. A first motor (105) is fixedly installed on the top of the reaction tank (1), and a central shaft (106) is fixedly connected to the output end of the first motor (105). A feed inlet (107) is provided on the top of the reaction tank (1), and a discharge outlet (123) is provided on the outer wall of the reaction tank (1). The feed inlet (107) is located above the first reaction chamber (102), and the central shaft (106) is rotatably fitted on the inner wall of the reaction tank (1). Two fixed shafts (108) are fixedly connected to the circumferential side of the central shaft (106). Both fixed shafts (108) are rotatably fitted with conical teeth (1081) on their circumferential sides. A plurality of flipping blades (1082) are fixedly connected to one side of the conical teeth (1081). The plurality of flipping blades (1082) are arranged in a circumferential array on the circumferential side of the fixed shaft (108). The plurality of flipping blades (1082) and the fixed shaft (108) are rotatably fitted together. The bottom of the third reaction chamber (104) is fixedly installed with a main bevel tooth (109), which meshes with two secondary bevel teeth (1081).

2. The multi-zone temperature-controlled bioreactor for improving the transport efficiency of phytosterol esterase synthesis according to claim 1, characterized in that: The main bevel tooth (109) has a central groove (1091) at the top center, and the bottom of the central shaft (106) and the central groove (1091) are rotatably engaged.

3. The multi-zone temperature-controlled bioreactor for improving the transport efficiency of phytosterol esterase synthesis according to claim 1, characterized in that: The inner wall of the reaction vessel (1) is provided with a limiting groove (110), and the ends of the two fixed shafts (108) are slidably fitted on the inner wall of the limiting groove (110).

4. The multi-zone temperature-controlled bioreactor for improving the transport efficiency of phytosterol esterase synthesis according to claim 2, characterized in that: Several sets of stirring shafts (1061) are fixedly connected to the circumferential side of the central shaft (106). Each set of stirring shafts (1061) has a certain number of shafts. Each set of stirring shafts (1061) is respectively set in the first reaction chamber (102), the second reaction chamber (103) and the third reaction chamber (104).

5. The multi-zone temperature-controlled bioreactor for improving the transport efficiency of phytosterol esterase synthesis according to claim 4, characterized in that: The reaction vessel (1) is provided with a first temperature control coil (111), a second temperature control coil (112) and a third temperature control coil (113) in sequence from top to bottom around the circumference. The first temperature control coil (111) is located outside the first reaction chamber (102), the second temperature control coil (112) is located outside the second reaction chamber (103), and the third temperature control coil (113) is located outside the third reaction chamber (104).

6. The multi-zone temperature-controlled bioreactor for improving the transport efficiency of phytosterol esterase synthesis according to claim 5, characterized in that: The outer wall of the reaction vessel (1) is provided with a first input three-way valve (114), a second input three-way valve (115) and a third input three-way valve (116) from top to bottom. The first input three-way valve (114) is connected to the inlet end of the first temperature control coil (111), the second input three-way valve (115) is connected to the inlet end of the second temperature control coil (112), and the third input three-way valve (116) is connected to the inlet end of the third temperature control coil (113).

7. The multi-zone temperature-controlled bioreactor for improving the transport efficiency of phytosterol esterase synthesis according to claim 6, characterized in that: The outer wall of the reaction vessel (1) is provided with a first output three-way valve (117), a second output three-way valve (118) and a third output three-way valve (119) from top to bottom. The first output three-way valve (117) is connected to the output end of the first temperature control coil (111), the second output three-way valve (118) is connected to the output end of the second temperature control coil (112), and the third output three-way valve (119) is connected to the output end of the third temperature control coil (113).

8. The multi-zone temperature-controlled bioreactor for improving the transport efficiency of phytosterol esterase synthesis according to claim 7, characterized in that: The first input three-way valve (114), the second input three-way valve (115) and the third input three-way valve (116) are respectively connected to the output ends of the external cold medium tank and the hot medium tank, and the first output three-way valve (117), the second output three-way valve (118) and the third output three-way valve (119) are respectively connected to the input ends of the external cold medium tank and the hot medium tank.

9. The multi-zone temperature-controlled bioreactor for improving the transport efficiency of phytosterol esterase synthesis according to claim 7, characterized in that: The outer wall of the reaction tank (1) is provided with a first transmission pipe (120) and a second transmission pipe (121) from top to bottom. The two ends of the first transmission pipe (120) are respectively connected to the first reaction chamber (102) and the second reaction chamber (103), and the two ends of the second transmission pipe (121) are respectively connected to the second reaction chamber (103) and the third reaction chamber (104). A second motor (122) is fixedly installed on the outer wall of the first transmission pipe (120) and the second transmission pipe (121). The output ends of the two second motors (122) are fixedly connected to a rotating shaft (1221). Spiral auger blades (1222) are fixedly connected to the circumferential sides of the two rotating shafts (1221). The two spiral auger blades (1222) are respectively attached to the inner wall of the first transmission pipe (120) and the second transmission pipe (121).

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