An enzymatic reaction system for high-value preparation of cottonseed protein

Abstract

The application discloses a kind of enzymatic reaction systems of high-value preparation of cottonseed protein, including reaction tank main body, stirring mechanism, ultrasonic device, enzyme preparation spraying and distribution component, heating / cooling jacket and multi-parameter online monitoring and control system.The system is controlled and dynamically adjusted by central controller according to the preset multi-stage enzymolysis process procedure, stirring speed, ultrasonic power, enzyme liquid addition and temperature, and realizes self-adaptive adjustment based on the pH, temperature and product concentration data of online monitoring.Its stirring paddle adopts radial and axial flow combination design, ultrasonic transducer is asymmetrically arranged, enzyme liquid is sprayed into high-speed stirring zone through atomizing nozzle of specific angle, to realize efficient mixing.The system can also integrate defoaming, membrane filtration reflux, inert gas protection and multi-tank combination module.The application realizes the precise, efficient, stable and intelligent control of cottonseed protein enzymolysis process, significantly improves product yield, quality consistency and production efficiency.

Description

Technical Field

[0001] This invention relates to the field of bioengineering, and more particularly to an enzymatic hydrolysis reaction system for the high-value preparation of cottonseed protein. Background Technology

[0002] Cottonseed is an important plant protein resource, rich in protein and with a balanced amino acid composition, possessing high nutritional and functional value. However, traditional cottonseed protein processing mainly employs physical or chemical methods, which suffer from limited extraction efficiency, high protein denaturation, and insufficient functional modification. Furthermore, it is difficult to effectively remove or inactivate anti-nutritional factors such as gossypol, resulting in low product added value and limited application scope. In recent years, enzymatic hydrolysis, due to its mild conditions, high specificity, and ability to improve protein functional properties, has been regarded as a key technological pathway for achieving high-value utilization of cottonseed protein.

[0003] However, in practical industrial applications, existing enzymatic hydrolysis processes typically suffer from a series of engineering and technical bottlenecks, including insufficient hydrolysis efficiency and stability, low precision in dynamic control of reaction conditions (such as pH, temperature, and mixing uniformity), inadequate contact between the enzyme and substrate, and easy impairment of product activity. This results in poor controllability of the hydrolysis process and large fluctuations in the yield and quality of the target product, limiting the large-scale and standardized production of high-end products such as high-quality cottonseed protein peptides. Therefore, developing a highly efficient, stable, and controllable dedicated enzymatic hydrolysis reaction system is of great significance for enhancing the added value of cottonseed protein and promoting industrial technology upgrading. Summary of the Invention

[0004] This invention specifically relates to an enzymatic hydrolysis reaction system for the high-value preparation of cottonseed protein. The system aims to achieve closed-loop control and dynamic adjustment of stirring speed, ultrasonic power, enzyme addition, and temperature through a central controller based on a preset multi-stage enzymatic hydrolysis process, and to achieve adaptive adjustment based on online monitoring of pH, temperature, and product concentration data. To achieve the above objectives, the specific technical solution of the enzymatic hydrolysis reaction system for the high-value preparation of cottonseed protein according to this invention is as follows: An enzymatic hydrolysis reaction system for the high-value preparation of cottonseed protein includes: The main body of the reaction vessel has a feed inlet and an enzyme inlet at the top, and a discharge outlet at the bottom. The stirring mechanism is coaxially arranged inside the main body of the reaction vessel, and includes a drive motor, a stirring shaft connected to the output shaft of the drive motor, and multiple layers of stirring blades fixed on the stirring shaft. An ultrasonic device includes at least one ultrasonic transducer fixed to the outer side wall of the main body of the reaction vessel and an ultrasonic generator electrically connected to the ultrasonic transducer, wherein the emitting surface of the ultrasonic transducer is in communication with the inner cavity of the main body of the reaction vessel. The enzyme preparation spraying and distribution assembly includes an annular spray pipe located at the top of the interior of the reaction vessel body. The annular spray pipe is connected to an external enzyme solution supply device via a pipeline, and its lower side is provided with multiple atomizing nozzles. Heating / cooling jacket integrated into the main body of the reaction vessel; The multi-parameter online monitoring and control system includes a pH sensor and a temperature sensor installed on the inner wall of the reaction vessel body; An online sampling and detection module is used to monitor the physicochemical parameters of the reaction system in real time; The central controller has its signal input terminal connected to the pH sensor, temperature sensor and online sampling and detection module, and its control output terminal connected to the speed control unit of the drive motor, the ultrasonic generator, the flow control unit of the enzyme solution supply device and the temperature control unit of the heating / cooling jacket. The central controller is configured to receive monitoring data from each sensor, and according to the preset enzymatic hydrolysis process, to perform closed-loop control and dynamic adjustment of the drive motor speed, ultrasonic generator power, enzyme solution addition rate, and the working status of the heating / cooling jacket.

[0005] Furthermore, the multi-layer stirring blades include at least one layer of radial flow blades located at the lower part of the stirring shaft and one layer of axial flow blades located at the upper part of the stirring shaft.

[0006] Furthermore, the number of ultrasonic transducers is multiple, and they are arranged asymmetrically along the circumference and / or axial direction of the reaction vessel body.

[0007] Furthermore, the online sampling and detection module is one or more combinations of an online rheometer, an online spectroscopic detection device, or an online chromatographic detection device.

[0008] Furthermore, the system also includes an automatic defoamer dosing device connected to the central controller, with its nozzle positioned above the liquid level inside the reaction tank body.

[0009] Furthermore, the enzymatic hydrolysis process program preset in the central controller is a multi-stage program, including at least an ultrasonic pretreatment stage, a main enzymatic hydrolysis stage, and a modification reaction stage executed sequentially, with each stage having independent pH value, temperature, stirring speed, and ultrasonic action parameter settings.

[0010] Furthermore, the central controller is also configured to: compare the real-time product concentration data fed back by the online sampling and detection module (11) with the preset target threshold of the current reaction stage, and automatically adjust the duration of the current stage based on the comparison result.

[0011] Furthermore, the annular spray pipe is arranged around the stirring shaft, and the spray direction of the atomizing nozzle forms an angle of 30°-60° with the horizontal plane and points towards the stirring shaft.

[0012] Furthermore, the system also includes a membrane filtration separation unit, the inlet of which is connected to the outlet of the main body of the reaction vessel, and the outlet of which is connected to the inlet of the main body of the reaction vessel via a reflux pipeline; the central controller is also connected to the flow control valve of the membrane filtration separation unit.

[0013] Furthermore, the system is composed of two or more reaction vessel bodies connected in series and / or in parallel via pipelines; The central controller is also connected to an inert gas supply device, the gas supply device having a vent pipe extending into the bottom of the reaction vessel body; the central controller is configured to control the introduction of inert gas before and during the reaction to maintain an inert atmosphere in the top space of the reaction vessel.

[0014] Compared with existing technologies, the advantages of this invention lie in its closed-loop control and dynamic adjustment of the composite physical field consisting of a stirring mechanism, ultrasonic device, enzyme preparation spraying assembly, and heating / cooling jacket, based on a preset multi-stage enzymatic hydrolysis process program by a central controller. Combined with real-time feedback from pH and temperature sensors and an online sampling and detection module, this achieves precise optimization and end-to-end stability of reaction conditions, significantly improving enzymatic hydrolysis efficiency and product consistency. Its unique radial and axial combined stirring blades, working in synergy with asymmetrically arranged ultrasonic transducers, greatly enhance mass transfer and mixing effects. Furthermore, the adaptive control mechanism based on real-time product concentration intelligently determines the reaction endpoint, effectively ensuring stable product quality. In addition, the integrated design details such as automatic defoamer addition, enzyme solution atomization spray directed towards the stirring shaft, membrane filtration reflux, and inert gas protection further improve the reliability of system operation, raw material utilization, and avoid oxidation side reactions. The entire system is designed to be modular and scalable, and can be flexibly adapted to different production scales by connecting multiple tanks in series or in parallel, providing an efficient, intelligent and stable dedicated equipment solution for the high-value and large-scale preparation of cottonseed protein. Attached Figure Description

[0015] Figure 1 This is a flowchart illustrating the working principle of the present invention. Detailed Implementation

[0016] To make the objectives, technical solutions, and advantages of the present invention clearer, the following description, in conjunction with preferred embodiments and appendices, provides further details. Figure 1 This invention will be described in detail. This embodiment is only for explaining the invention and is not intended to limit the scope of protection of the invention.

[0017] Example 1 This embodiment details an enzymatic hydrolysis reaction system for the high-value preparation of cottonseed protein, comprising: a reaction tank body with a feed inlet and an enzyme inlet at the top and a discharge outlet at the bottom; a stirring mechanism coaxially disposed inside the reaction tank body, including a drive motor, a stirring shaft connected to the output shaft of the drive motor, and multi-layer stirring blades fixed on the stirring shaft; an ultrasonic device including at least one ultrasonic transducer fixed to the outer wall of the reaction tank body and an ultrasonic generator electrically connected to the ultrasonic transducer, wherein the emitting surface of the ultrasonic transducer communicates with the inner cavity of the reaction tank body; and an enzyme preparation spraying and distribution assembly including an annular spray pipe disposed at the top of the inside of the reaction tank body, the annular spray pipe being connected to an external enzyme solution supply device via a pipeline, and having multiple atomizing nozzles on its lower side; and a collection... The system comprises a heating / cooling jacket on the main body of the reaction vessel; a multi-parameter online monitoring and control system including pH and temperature sensors installed on the inner wall of the reaction vessel; an online sampling and detection module for real-time monitoring of the physicochemical parameters of the reaction system; and a central controller whose signal input is connected to the pH sensor, temperature sensor, and online sampling and detection module, and whose control output is connected to the speed control unit of the drive motor, the ultrasonic generator, the flow control unit of the enzyme supply device, and the temperature control unit of the heating / cooling jacket. The central controller is configured to receive monitoring data from each sensor, and according to a preset enzymatic hydrolysis process, to perform closed-loop control and dynamic adjustment of the drive motor speed, the ultrasonic generator power, the enzyme addition rate, and the working status of the heating / cooling jacket. In this embodiment, the core of the enzymatic hydrolysis reaction system is a reaction vessel body made of stainless steel, with a quick-opening manhole, a dedicated enzyme inlet, and a sensor interface at the top, and a conical outlet at the bottom. An agitation mechanism is coaxially mounted inside the tank, consisting of a variable frequency drive motor, a sealed agitator shaft penetrating the top of the tank, and multiple layers of agitator blades fixed to the shaft. A heating / cooling jacket is installed on the outer wall of the tank. Multiple ultrasonic transducers are fixedly installed at a certain angle outside the jacket and connected to independent ultrasonic generators via cables. A ring-shaped spray pipe made of annular stainless steel tubing is suspended from the top of the tank, connected to an external metering diaphragm pump via piping. Multiple precision atomizing nozzles are evenly distributed below the ring pipe. A pH sensor and a temperature sensor are fixedly installed on the inner wall of the tank, and an online sampling and detection module capable of automatically and periodically drawing trace amounts of reaction solution is connected to the side. All sensors and actuators are connected to a central controller located in the electrical control cabinet via signal lines. The controller has a pre-programmed and stored enzymatic hydrolysis process program for cottonseed protein peptide production. This embodiment constructs a highly integrated and automated dedicated enzymatic hydrolysis reaction platform. By integrating functional units such as physical stirring, ultrasonic cavitation, precise temperature control, uniform enzyme addition, and online monitoring into one unit, and using a central controller for unified scheduling and closed-loop control, the traditional enzymatic hydrolysis reaction model, which relies on human experience, has been completely transformed, characterized by extensive condition control and disconnected processes.The system can accurately reproduce and stably execute complex optimized processes, providing core equipment support for the efficient and controllable enzymatic hydrolysis of cottonseed protein, fundamentally ensuring the consistency of product quality and the repeatability of the process.

[0018] In this embodiment, the multi-layered impellers fixed to the stirring shaft have a specific combination: the bottom layer consists of two six-bladed disc turbine impellers, i.e., radial flow impellers, whose strong shear force is responsible for fully dispersing the deposited protein powder at the bottom and forming radial flow; the middle and upper layers are folded-blade inclined impellers, i.e., axial flow impellers, installed at a 45° angle, whose main function is to convert the radial flow into a strong axial up-and-down circulation, promoting uniform mixing of the materials in the tank. This multi-layered combined stirring design of "lower radial, upper axial" optimizes the flow field inside the tank. The lower radial flow impellers ensure sufficient suspension and initial dispersion of solid particles, preventing sedimentation; the upper axial flow impellers form an efficient overall circulation, eliminating gradients in temperature, concentration, and pH. The synergistic effect of both allows the enzyme and substrate molecules to come into rapid and uniform contact throughout the reaction system, greatly improving mass transfer efficiency and creating ideal hydrodynamic conditions for uniform and efficient enzymatic hydrolysis.

[0019] In this embodiment, multiple ultrasonic transducers are installed asymmetrically. Two are installed in the lower part of the tank, arranged diagonally at 180 degrees but with a height difference in the axial direction; the third is installed at the top, offset circumferentially from the two transducers below. This asymmetrical arrangement in both the circumferential and axial directions results in a complex and interwoven sound field distribution of ultrasonic energy in the reaction medium. The asymmetrical arrangement of the ultrasonic transducers effectively breaks the symmetry of the sound field, avoiding fixed "dead zones" caused by beam interference. It can generate a more uniform and irregular cavitation effect and microjets throughout the entire reaction volume, making the ultrasonic waves' effects on protein particle fragmentation, enzyme molecule conformation activation, and boundary layer disturbance more comprehensive and thorough. This significantly improves the overall efficiency of ultrasonic treatment, ensuring that materials in all areas of the tank receive effective ultrasonic assistance, thereby synergistically improving the overall enzymatic hydrolysis rate and uniformity.

[0020] In this embodiment, the online sampling and detection module consists of an automatic sampling pump, a miniature flow cell, and an online near-infrared spectrometer. The sampling pump periodically draws a small amount of reaction solution from the reaction vessel, flows it through the flow cell for rapid spectral scanning, and then returns it to the vessel. The spectrometer analyzes the spectral data in real time, calculates the relative concentration of specific peptides in the current reaction solution using an established model, and transmits this data to the central controller in real time. The introduction of an online spectral detection device replaces the traditional manual timed sampling and offline laboratory analysis method. It enables real-time, continuous, and non-destructive monitoring of key product indicators. This not only reflects the dynamic changes in the reaction process in real time, providing the most direct data basis for the dynamic adjustment of the central controller, but more importantly, it transforms the determination of the reaction endpoint from being based on "fixed time" to being based on "target product concentration," providing a core technical means for achieving precise process control and ensuring absolute consistency in the quality of different batches of products.

[0021] In this embodiment, a small defoamer storage tank and a micro-metering pump controlled by a central controller are added to the top of the reaction vessel, forming an automatic defoamer addition device. A thin dropper is connected to the end of the metering pump's outlet pipe, with its outlet positioned precisely above the normal liquid level inside the vessel. When the online monitoring system detects foam generation, the central controller instructs the metering pump to inject a trace amount of food-grade defoamer. The introduction of the automatic defoamer addition device solves many problems caused by the large amount of foam generated during protein decomposition in the enzymatic hydrolysis process. Automatic addition can suppress foam in a timely and precise manner, preventing the reduction of effective volume, material spillage and contamination, and inaccurate pH and temperature sensor measurements due to foam entrainment caused by excessive foam. This ensures the stable and safe operation of the reaction process and also guarantees the reliability of other online monitoring data, serving as an important auxiliary design for maintaining the long-term stable and automated operation of the system.

[0022] In the central controller program of this embodiment, the preset enzymatic hydrolysis process is clearly divided into three stages: The first stage is ultrasonic pretreatment, where a specific temperature and pH are set, ultrasonic waves are activated, and low-speed stirring is performed to initially open up the protein structure. The second stage is the main enzymatic hydrolysis, where the temperature and pH are adjusted to the optimal level, ultrasonic waves are stopped, high-speed stirring is initiated, and protease is continuously added according to a preset curve. The third stage is the modification reaction, where the temperature and pH conditions are changed, and another enzyme is added for flavor or functional modification. This multi-stage programmed control strategy perfectly matches the scientific path of optimal enzymatic hydrolysis of cottonseed protein. It is no longer a single-condition isothermal and pH reaction, but rather a step-by-step and orderly creation of the optimal environment based on enzymatic hydrolysis kinetics and the characteristics of different enzymes. "Ultrasonic pretreatment" clears obstacles for subsequent enzymatic hydrolysis; the "main enzymatic hydrolysis" stage carries out core transformation under optimal conditions; and the "modification reaction" stage focuses on improving product characteristics. This refined process control maximizes the role of the complex enzyme and is the key process logic for achieving high-yield, high-quality, and high-functionality products.

[0023] This embodiment combines online detection and multi-stage program control. During the main enzymatic hydrolysis stage, the central controller continuously receives target peptide concentration data from the online detector. A target concentration threshold is set for this stage in the program. The controller compares the monitored concentration with the threshold in real time. If the concentration is not reached by the preset time endpoint, the system automatically extends the reaction time for that stage until the concentration reaches the target before triggering the next stage. Conversely, if the target is reached prematurely, the system immediately terminates the current stage and jumps to the next stage. This adaptive control mechanism based on real-time feedback endows the system with strong anti-interference and intelligent capabilities. It can automatically compensate for the impact of uncontrollable factors such as batch differences in raw materials, enzyme activity fluctuations, and changes in ambient temperature, ensuring that each batch of reaction achieves the exact same endpoint product standard. This completely solves the product quality fluctuation problem caused by the traditional fixed-time method, achieving a leap from "process control" to "result control," and providing unparalleled stability assurance for the production of specific functional peptide products with strict quality standards.

[0024] In this embodiment, the diameter of the annular spray pipe is slightly smaller than the inner diameter of the tank. It is fixed to the center of the tank top by a hanger and surrounds the stirring shaft. The multiple atomizing nozzles below it are not vertically downwards, but are uniformly installed at a 45-degree angle, with the spray axis of all nozzles pointing towards the stirring shaft at the center of the tank. This directional spray design at a specific angle is ingenious. When the enzyme solution is sprayed out in a mist, its direction is directly towards the area of ​​the high-speed rotating stirring shaft, which is where the fluid shear force and turbulence intensity are highest inside the tank. The atomized enzyme solution is instantly drawn into a strong vortex, achieving uniform micro-scale mixing with the main material in a very short time. This avoids problems such as "wall adhesion" loss caused by direct spraying of the enzyme solution onto the tank wall or local liquid surface, and enzyme molecule aggregation and inactivation caused by excessively high local concentrations. This greatly improves the utilization efficiency of expensive enzyme preparations and ensures that the enzymatic reaction starts uniformly from the initial moment.

[0025] In this embodiment, the system's outlet is connected to an external membrane filtration separation unit via a pipeline. After the enzymatic hydrolysis reaction is complete, the material is pumped into this unit. Peptides of the target molecular weight are collected as the product, while large protein molecules that are not fully hydrolyzed are directly pumped back to the inlet of the main reaction tank via a reflux pipeline, where they are mixed with the next batch of raw materials for further enzymatic hydrolysis. The integrated membrane filtration and reflux unit achieves a closed-loop cycle of "reaction-separation-re-reaction." It can selectively retain and recover large molecular substrates that are not fully utilized in the first reaction and reintroduce them into the reaction system, thereby increasing the total conversion rate of the raw materials to near the theoretical limit and significantly reducing raw material costs. At the same time, this design allows for "full-spectrum" enzymatic hydrolysis in the main tank, followed by precise acquisition of products within the target molecular weight range through membrane separation. This achieves integrated process enhancement and refined product separation, improving the system's economic efficiency and technological added value.

[0026] In the industrial version of this embodiment, the system consists of three identical reaction tanks connected in series via pipes and valves. The first tank primarily performs ultrasonic pretreatment and the initial stage of main enzymatic hydrolysis. The material then overflows or is pumped into the second tank to complete the main enzymatic hydrolysis, and finally enters the third tank for modification reactions and insulation. Each tank has an independent control system, but is coordinated by a central control center. The modular design of multiple tanks connected in series or parallel gives the system extremely high production flexibility and scalability. The series mode enables true continuous production, greatly increasing capacity and creating a reactor effect similar to "piston flow," making process control more precise. The parallel mode is suitable for large-scale batch production, increasing single-batch output. This design allows the system to be seamlessly scaled up from the laboratory level to the industrial production level, shortening the R&D to industrialization cycle and facilitating flexible adjustments to production lines and capacity according to market demand.

[0027] In this embodiment, the system is equipped with an inert gas supply device, whose delivery pipe is inserted from the top of the tank and extends to the bottom of the reaction vessel. Before each feeding begins, the central controller activates this device to introduce nitrogen gas from the bottom, displacing the air inside the tank through the top exhaust valve. Throughout the subsequent reaction process, the system maintains a slightly positive pressure nitrogen atmosphere. The inert gas protection system addresses the challenge of easy oxidation of cottonseed protein and enzymatic hydrolysis products. Through gas replacement before the reaction and atmosphere maintenance during the reaction, oxygen in the tank top space and dissolved in the feed liquid can be effectively eliminated. This prevents oxidative denaturation of proteins and peptides, maintaining the natural activity and nutritional value of the products; at the same time, it inhibits side reactions such as lipid oxidation, improving the flavor and color of the final product. This is an important guarantee for producing high-quality, highly stable protein products.

[0028] Detailed working principle and operation process of this patented system Phase 1: System Preparation and Feeding Before system startup, operators set the process formula and target parameters for this production through the human-machine interface, and the central controller loads the corresponding multi-stage enzymatic hydrolysis program. Then, the prepared cottonseed protein raw material solution is pumped into the main body of the reaction tank through the top inlet. After feeding, the central controller first activates the inert gas supply device, introducing nitrogen from the bottom of the tank to continuously replace the air at the top until the set inert atmosphere is reached and maintained to eliminate the risk of oxidation. The heating / cooling jacket starts working, adjusting the temperature of the liquid to the preset initial temperature. The stirring mechanism operates at a low starting speed to ensure initial homogenization of the materials.

[0029] Second stage: Ultrasonic pretreatment stage Once the reaction system reaches the preset initial temperature, the system automatically enters the "ultrasonic pretreatment" stage. The central controller precisely adjusts the pH and temperature of the system to the optimal pretreatment values ​​according to the program settings. At this time, the ultrasonic generator starts, driving the asymmetrically arranged ultrasonic transducers to emit ultrasonic waves of specific power and frequency into the reaction system, generating cavitation and mechanical oscillation. Simultaneously, the stirring mechanism maintains a low to medium speed. Ultrasonic cavitation effectively disrupts the tightly aggregated structure of cottonseed proteins, exposing their cleavage sites and passivating some endogenous anti-nutritional factors. This synergistic effect lays the foundation for subsequent efficient enzymatic hydrolysis.

[0030] Third stage: Main enzymatic hydrolysis stage After pretreatment, the system automatically switches to the core "main enzymatic hydrolysis" stage. The central controller precisely adjusts the temperature and pH to the optimal conditions for main enzymatic hydrolysis and may disable ultrasound. The stirring speed is increased to high speed, creating a strong radial and axial composite flow field to ensure thorough mixing of the materials. At this time, the enzyme preparation spraying and distribution components are activated, and the metering pump delivers the enzyme solution to the annular spray pipe. Through multiple atomizing nozzles, the enzyme solution is evenly sprayed into the high-speed rotating stirring shaft area at a specific angle, achieving instantaneous and efficient mixing of the enzyme and substrate. Throughout the main enzymatic hydrolysis process, pH and temperature sensors continuously monitor environmental parameters, and the central controller maintains stable conditions through closed-loop control by adjusting the jacket and acid-base addition unit.

[0031] Phase 4: Online Monitoring and Adaptive Control During the main enzymatic hydrolysis stage, online sampling and detection modules (such as online spectrometers) automatically sample and analyze the reaction system at regular intervals, feeding back the concentration data of key products (such as specific functional peptides) to the central controller in real time. The controller compares this real-time data with the preset target concentration threshold for the current stage. If the preset time point is reached but the concentration does not meet the target, the system automatically extends the reaction time for this stage; if the target is met ahead of schedule, the stage is immediately terminated, and the process jumps to the next step. This adaptive control based on real-time results ensures absolute consistency in the quality of the final product from different batches of raw materials. Simultaneously, if the foam sensor detects an increase in foam on the liquid surface, the automatic defoamer addition device will immediately inject a trace amount of defoamer to ensure process safety.

[0032] Phase 5: Modification Reaction and Post-treatment Once the primary enzymatic hydrolysis target is achieved, the system can enter the "modification reaction" stage. The central controller adjusts the temperature and pH to another set of values, and another flavor protease or modifying enzyme can be added via the spray assembly. The stirring speed is adjusted accordingly to complete the final flavor improvement or functional modification of the product. After the reaction is complete, stirring stops, and the heating / cooling jacket is activated to cool the material to the discharge temperature.

[0033] Phase 6: Discharge, Separation and Recycling The discharge valve opens, and the reaction-completed liquid is transported to the membrane filtration separation unit. Here, the permeate (target small molecule peptide) is collected as the product, while the large molecules in the retentate that have not been completely enzymatically hydrolyzed are pumped back to the main body of the reaction tank through the reflux pipeline. They can be mixed with the next batch of fresh material for further enzymatic hydrolysis, thereby achieving near-complete utilization of the raw materials and significantly improving the yield.

[0034] Phase 7: Cleaning and Preparation After one batch of production is completed, the system can execute an automatic cleaning procedure (CIP) to prepare for the next batch. In the configuration of multiple tanks connected in series, the material flows continuously in multiple reaction tanks, completing different stages such as pretreatment, main enzymatic hydrolysis, and modification, thereby achieving continuous production and significantly improving efficiency.

[0035] It is understood that the present invention has been described through some embodiments, and those skilled in the art will recognize that various changes or equivalent substitutions can be made to these features and embodiments without departing from the spirit and scope of the invention. Furthermore, under the teachings of the present invention, these features and embodiments can be modified to adapt to specific situations and materials without departing from the spirit and scope of the invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the protection scope of the present invention.

Claims

1. An enzymatic hydrolysis reaction system for the high-value preparation of cottonseed protein, characterized in that, include: The main body of the reaction vessel has a feed inlet and an enzyme inlet at the top, and a discharge outlet at the bottom. The stirring mechanism is coaxially arranged inside the main body of the reaction vessel, and includes a drive motor, a stirring shaft connected to the output shaft of the drive motor, and multiple layers of stirring blades fixed on the stirring shaft. An ultrasonic device includes at least one ultrasonic transducer fixed to the outer side wall of the main body of the reaction vessel and an ultrasonic generator electrically connected to the ultrasonic transducer, wherein the emitting surface of the ultrasonic transducer is in communication with the inner cavity of the main body of the reaction vessel. The enzyme preparation spraying and distribution assembly includes an annular spray pipe located at the top of the interior of the reaction vessel body. The annular spray pipe is connected to an external enzyme solution supply device via a pipeline, and its lower side is provided with multiple atomizing nozzles. Heating / cooling jacket integrated into the main body of the reaction vessel; The multi-parameter online monitoring and control system includes a pH sensor and a temperature sensor installed on the inner wall of the reaction vessel body; An online sampling and detection module is used to monitor the physicochemical parameters of the reaction system in real time; The central controller has its signal input terminal connected to the pH sensor, temperature sensor and online sampling and detection module, and its control output terminal connected to the speed control unit of the drive motor, the ultrasonic generator, the flow control unit of the enzyme solution supply device and the temperature control unit of the heating / cooling jacket. The central controller is configured to receive monitoring data from each sensor, and according to the preset enzymatic hydrolysis process, to perform closed-loop control and dynamic adjustment of the drive motor speed, ultrasonic generator power, enzyme solution addition rate, and the working status of the heating / cooling jacket.

2. The enzymatic hydrolysis reaction system for high-value preparation of cottonseed protein according to claim 1, characterized in that, The multi-layered stirring blades include at least one layer of radial flow blades located at the lower part of the stirring shaft and one layer of axial flow blades located at the upper part of the stirring shaft.

3. The enzymatic hydrolysis reaction system for high-value preparation of cottonseed protein according to claim 1, characterized in that, The ultrasonic transducers are multiple and are arranged asymmetrically along the circumference and / or axial direction of the reaction vessel body.

4. The enzymatic hydrolysis reaction system for high-value preparation of cottonseed protein according to claim 1, characterized in that, The online sampling and detection module is one or more combinations of an online rheometer, an online spectrometer, or an online chromatographic detector.

5. The enzymatic hydrolysis reaction system for high-value preparation of cottonseed protein according to claim 1, characterized in that, The system also includes an automatic defoamer dosing device connected to the central controller, with its nozzle positioned above the liquid level inside the reaction tank.

6. The enzymatic hydrolysis reaction system for high-value preparation of cottonseed protein according to claim 1, characterized in that, The enzymatic hydrolysis process program preset in the central controller is a multi-stage program, which includes at least an ultrasonic pretreatment stage, a main enzymatic hydrolysis stage, and a modification reaction stage executed sequentially. Each stage has independent settings for pH value, temperature, stirring speed, and ultrasonic action parameters.

7. The enzymatic hydrolysis reaction system for high-value preparation of cottonseed protein according to claims 4 and 6, characterized in that, The central controller is also configured to: compare the real-time product concentration data fed back by the online sampling and detection module (11) with the preset target threshold of the current reaction stage, and automatically adjust the duration of the current stage based on the comparison result.

8. The enzymatic hydrolysis reaction system for high-value preparation of cottonseed protein according to claim 1, characterized in that, The annular spray pipe is arranged around the stirring shaft, and the spray direction of the atomizing nozzle is at an angle of 30°-60° with the horizontal plane and points towards the stirring shaft.

9. The enzymatic hydrolysis reaction system for high-value preparation of cottonseed protein according to claim 1, characterized in that, The system also includes a membrane filtration separation unit, the inlet of which is connected to the outlet of the main body of the reaction vessel, and the outlet of which is connected to the inlet of the main body of the reaction vessel via a reflux pipeline; the central controller is also connected to the flow control valve of the membrane filtration separation unit.

10. The enzymatic hydrolysis reaction system for high-value preparation of cottonseed protein according to claim 1, characterized in that, The system consists of two or more reaction vessel bodies connected in series and / or in parallel via pipelines; The central controller is also connected to an inert gas supply device, the gas supply device having a vent pipe extending into the bottom of the reaction vessel body; the central controller is configured to control the introduction of inert gas before and during the reaction to maintain an inert atmosphere in the top space of the reaction vessel.

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