A composition containing fluorine-containing chrysanthemic acid diester or KII-9396 and use thereof

Abstract

The present application relates to a kind of composition containing fluorine mite double ether or KII-9396 and its application.The compound I has synergistic effect with fluorine mite double ether or KII-9396, can improve pest control effect, reduce application amount, reduce use frequency, reduce use cost.

Description

[0001] This application is a divisional application of application number 202210249245.2, filed on March 14, 2022, entitled "A composition containing fluorinated acaricide or KII-9396 and its application". Technical Field

[0002] This invention belongs to the technical field of pesticide compound formulation, and relates to compositions containing a biologically effective amount of compound I and flufenoxuron or KII-9396, and methods for using them to control pests in agricultural and non-agricultural environments. Background Technology

[0003] In recent years, the occurrence of various crop pests has shown a trend of increasing severity year by year, with the affected area growing annually, causing enormous damage to crops and leading to problems such as reduced crop yields and increased agricultural production costs. To improve pest control, farmers often increase pesticide dosage or arbitrarily mix pesticides. This unscientific use of pesticides not only fails to achieve synergistic effects but also leads to pesticide waste, excessive residues, environmental pollution, and the development of pesticide resistance in pests. Therefore, there is an urgent need to develop a synergistic combination for pests that can broaden the range of target pests, reduce pesticide dosage, and delay the development of resistance.

[0004] In actual agricultural production, the long-term, continuous use of the same pesticide quickly leads to pesticide resistance in pests, resulting in reduced efficacy, increased pesticide usage, and exacerbated pesticide residues in agricultural products and damage to the ecological environment. Combining insecticides with completely different mechanisms of action is an effective way to delay the development of pesticide resistance, broaden the pesticide spectrum, extend the pesticide's lifespan, and reduce pesticide usage. However, determining how to combine the pesticides to achieve synergistic rather than antagonistic effects remains a significant challenge.

[0005] Patent document CN 111909143 A discloses compound I, which has broad-spectrum insecticidal activity, especially effective against pests of the orders Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, and mites. However, it does not disclose how to combine it with other drugs to achieve synergistic effects.

[0006] The structural formula of compound I is shown below. .

[0007] I Summary of the Invention

[0008] To overcome the shortcomings of the prior art, the present invention first provides a composition comprising compound I and compound II, wherein compound II is flufenoxuron, KII-9396, or a combination thereof. The structure of compound I is shown below. , The KII-9396 is (RS)-[5-[4-chloro-2-fluoro-5-[(2,2,2-trifluoroethyl)sulfinyl]phenoxy]pentyl]trifluoromethyl sulfide (CAS: 1472050-04-6).

[0009] According to an embodiment of the present invention, compound I was prepared by the method of Example 1 in patent document CN 111909143 A, the entire contents of which are incorporated herein by reference.

[0010] According to an embodiment of the present invention, the mass ratio of compound I to compound II is 80 to 1:1 to 80, preferably 50 to 1:1 to 50, and more preferably 10 to 1:1 to 10, for example 50:1, 40:1, 30:1, 20:1, 10:1, 5:1, 4:1, 3:1, 2:1, 1:1, 1:2, 1:3, 1:4, 1:5, 1:10, 1:20, 1:30, 1:40 or 1:50.

[0011] According to embodiments of the present invention, the composition can be prepared as a liquid formulation or a solid formulation.

[0012] According to embodiments of the present invention, the content range of the effective active ingredient in the formulation varies depending on the type of formulation. Generally, liquid formulations contain 1% to 60% of the effective active ingredient by weight, preferably 5% to 50%; solid formulations contain 5% to 80% of the effective active ingredient by weight, preferably 10% to 70%.

[0013] According to embodiments of the present invention, the composition further includes at least one of the following: deionized water, organic solvent, emulsifier, dispersant, wetting agent, thickener, defoamer, stabilizer, binder, disintegrant, antifreeze, anticaking agent, suspending agent, film-forming agent, preservative, colorant, polymeric capsule wall material, pH adjuster, or filler.

[0014] According to an embodiment of the present invention, the total mass of compound I and compound II in the composition is 1-80%, preferably 2-70%, or 5-65%; 10-60%, for example 1%, 5%, 10%, 15%, 20%, 25%, 30%, 35%, 40%, 45% or 50%, based on the total weight of the composition as 100%.

[0015] According to embodiments of the present invention, the composition can be diluted or used directly by the user before use. Its formulation can be prepared by processing methods known to those skilled in the art, namely, mixing the active ingredients (i.e., compounds I and II) with one or more of the following: deionized water, organic solvents, emulsifiers, dispersants, wetting agents, thickeners, defoamers, stabilizers, binders, disintegrants, antifreeze agents, anticaking agents, suspending agents, film-forming agents, preservatives, colorants, polymeric capsule wall materials, pH adjusters, or fillers, to prepare the composition.

[0016] According to embodiments of the present invention, the composition can be formulated into various dosage forms, preferably including wettable powders, water-dispersible granules, suspensions, water-emulsions, suspension seed coatings, microcapsule suspensions, microcapsule suspension-suspension agents, emulsifiable concentrates, microemulsions, dispersible liquids, or granules.

[0017] According to an embodiment of the present invention, when the composition is made into a wettable powder, it comprises the following components and contents: compound I 1%~80%, compound II 1%~80%, dispersant 0%~10%, wetting agent 0%~8%, and the balance being filler.

[0018] Preferably, the compound I in the composition is 2%~70%, 5%~65%, 10%~60%, 15%~50%, etc.; Compound II is present in concentrations of 2%–70%, 5%–65%, 10%–60%, 15%–50%, etc. The dispersant concentration is 1%~8%, 2%~7%, 3-6%, or 4%~5%; The wetting agent is 1%~8%, 2%~7%, 3-6%, or 4%~5%. According to an embodiment of the present invention, when the composition is formulated into water-dispersible granules, it comprises the following components and contents: compound I 1%~80%, compound II 1%~80%, dispersant 0%~10%, wetting agent 0%~16%, disintegrant 0%~20%, and the balance being filler.

[0019] Preferably, the compound I in the composition is 2%~70%, 5%~65%, 10%~60%, 15%~50%, etc.; Compound II is present in concentrations of 2%–70%, 5%–65%, 10%–60%, 15%–50%, etc. The dispersant concentration is 1%~8%, 2%~7%, 3-6%, or 4%~5%; The wetting agent is 1%~8%, 2%~7%, 3-6% or 4%~5%; The disintegrant is 1%~18%, 2%~16%, 3-15%, 4%~12%, 5%~10%, or 6-8%.

[0020] According to an embodiment of the present invention, when the composition is made into a suspension, it comprises the following components and contents: compound I 1%~50%, compound II 1%~50%, dispersant 0%~5%, wetting agent 0%~8%, thickener 0%~0.3%, antifreeze agent 0%~5%, and the balance being deionized water.

[0021] Preferably, compound I in the composition is 2%~45%, 5%~40%, 10%~35%, 15%~30%, 20%~25%, etc.; Compound II is present in concentrations of 2%–45%, 5%–40%, 10%–35%, 15%–30%, and 20%–25%, etc. The dispersant is 1%~4% or 2%~3%; The wetting agent is 1%~8%, 2%~7%, 3-6% or 4%~5%; The thickener content is 0.1%~0.25%; Antifreeze 1%~4% or 2%~3%. According to an embodiment of the present invention, when the composition is formulated into an aqueous emulsion, it comprises the following components and contents: compound I 1%~50%, compound II 1%~50%, organic solvent 0%~95%, emulsifier 0%~10%, antifreeze 0%~5%, defoamer 0%~0.2%, thickener 0%~0.5%, and the balance being deionized water.

[0022] Preferably, compound I in the composition is 2%~45%, 5%~40%, 10%~35%, 15%~30%, 20%~25%, etc.; Compound II is present in concentrations of 2%–45%, 5%–40%, 10%–35%, 15%–30%, and 20%–25%, etc. The organic solvents are 1%~90%, 2%~80%, 5%~70%, 10%~60%, 15%~50%, 20%~50%, 30%~40%, etc. Emulsifiers: 1%~8%, 2%~7%, 3-6%, or 4%~5%; Antifreeze 1%~4% or 2%~3%; Defoamer 0.05%~0.1%, or The thickener is 0.1%~0.4% or 0.2%~0.3%. According to an embodiment of the present invention, when the composition is made into a suspension seed coating agent, it includes the following components and contents: compound I 1%~50%, compound II 1%~50%, dispersant 1%~12%, wetting agent 1%~10%, antifreeze agent 1~10%, anticaking agent 0.1~10%, suspending agent 0.1~5%, film-forming agent 1~10%, preservative 0.1~5%, colorant 1~30%, pH adjuster 0.1%~5%, thickener 0.1%~8%, and the balance is deionized water.

[0023] Preferably, compound I in the composition is 2%~45%, 5%~40%, 10%~35%, 15%~30%, 20%~25%, etc.; Compound II is present in concentrations of 2%–45%, 5%–40%, 10%–35%, 15%–30%, and 20%–25%, etc. The dispersant concentration is 2%~10%, 3%~8%, 4%~7%, or 5%~6%; The wetting agent is 1%~8%, 2%~7%, 3-6% or 4%~5%; Antifreeze is 1%~8%, 2%~7%, 3-6%, or 4%~5%; Anti-caking agents are available at concentrations of 0.5%–9%, ​​1%–8%, 2%–7%, 3–6%, or 4%–5%. The suspending agent is 0.2%~4%, 0.5%~3.5%, 1%~3%, 1.5%~2.5%, or 1.8%~2%; The film-forming agent is 2%~9%, 3%~8%, 4%~7%, or 5%~6%; The preservative content is 0.2%~4%, 0.5%~3.5%, 1%~3%, 1.5%~2.5%, or 1.8%~2%; The colorant is 2%~25%, 3%~20%, 5%~15%, 8%~12%, or 9%~10%; pH adjuster: 0.1%~5%, 0.2%~4%, 0.5%~3.5%, 1%~3%, 1.5%~2.5%, or 1.8%~2%; The thickener is 0.5%~7%, 1%~6%, 2%~5%, or 3%~4%.

[0024] According to an embodiment of the present invention, when the composition is formulated into a microcapsule suspension, it comprises the following components and contents: compound I 1%~50%, compound II 1%~50%, polymeric capsule wall material 1%~30%, dispersant 2%~10%, organic solvent 1%~50%, emulsifier 1%~7%, pH adjuster 0.1%~5%, defoamer 0.01%~2%, thickener 0.1%~8%, antifreeze agent 0.1%~8%, and the balance being deionized water.

[0025] Preferably, compound I in the composition is 2%~45%, 5%~40%, 10%~35%, 15%~30%, 20%~25%, etc.; Compound II is present in concentrations of 2%–45%, 5%–40%, 10%–35%, 15%–30%, and 20%–25%, etc. Polymer capsule wall material 2~25%, 5~20%, 6~18%, or 10~15%, etc.; The dispersant concentration is 3%~8%, 4%~7%, or 5%~6%; The organic solvents are 2%~45%, 5%~40%, 10%~35%, 15%~30%; or 20%~25%, etc. The emulsifier is 2%~6% or 4%~5%; pH adjuster: 0.2-4%, 0.5%-3.5%, 1%-3%, 1.5%-2.5%, or 1.8%-2%; The defoamer concentrations are 0.05~1.8%, 0.1%~1.5%, 0.5%~1.3%, and 0.8%~1.0%. The thickener is 0.2%~7%, 0.5%~6%, 1%~5%, 1.5%~4%, or 2%~3%; The antifreeze is 0.5%~7%, 1%~6%, 2%~5%, or 3%~4%.

[0026] According to an embodiment of the present invention, when the composition is prepared into a microcapsule suspension, it comprises the following components and contents: compound I 1%~50%, compound II 1%~50%, polymeric capsule wall material 1%~12%, dispersant 1%~12%, wetting agent 1%~8%, organic solvent 1%~50%, emulsifier 1%~8%, defoamer 0.01%~2%, thickener 0.1%~8%, pH adjuster 0.1%~5%, antifreeze agent 0.1%~8%, and the balance being deionized water.

[0027] According to an embodiment of the present invention, when the composition is prepared into an emulsifiable concentrate, it comprises the following components and contents: compound I 1%~50%, compound II 1%~50%, emulsifier 0~10%, antifreeze 0%~5%, stabilizer 0%~0.5%, and the balance being an organic solvent; Alternatively, Compound I 1%~50%, Compound II 1%~50%, Emulsifier 0%~12%, Stabilizer 0%~0.5%, with the remainder being organic solvent.

[0028] According to an embodiment of the present invention, when the composition is prepared into a microemulsion, it comprises the following components and contents: compound I 1%~50%, compound II 1%~50%, organic solvent 1%~50%, emulsifier 1%~30%, antifreeze 1%~10%, stabilizer 0.1%~5%, and the balance being deionized water.

[0029] According to an embodiment of the present invention, when the composition is prepared into a dispersible liquid, it comprises the following components and contents: compound I 1%~50%, compound II 1%~50%, emulsifier 1%~30%, antifreeze 1%~10%, stabilizer 0.1%~5%, and the balance being an organic solvent.

[0030] According to an embodiment of the present invention, when the composition is made into granules, it comprises the following components and contents: compound I 0.1%~10%, compound II 0.1%~10%, dispersant 0%~5%, wetting agent 0%~5%, binder 0%~3%, and the balance being filler.

[0031] According to an embodiment of the present invention, the emulsifier may be selected from at least one of the following: sodium lignosulfonate, agricultural emulsion, phenylphenol polyoxyethylene ether phosphate, tristyrylphenol polyoxyethylene ether phosphate triethanolamine salt, benzyl dimethylphenol polyoxyethylene ether, sorbitan fatty acid ester polyoxyethylene ether, sorbitan anhydride oleate (Span-80), fatty alcohol polyoxyethylene ether, sodium alkyl naphthalene sulfonate, sodium isooctanol succinate sulfonate, nonylphenol polyoxyethylene ether phosphate, and castor oil polyoxyethylene ether phosphate.

[0032] According to an embodiment of the present invention, the dispersant may be selected from at least one of the following: glycerol fatty acid polyoxyethylene ether, polyoxyethylene alkyl aryl ether, sodium lignosulfonate, naphthalene sulfonate formaldehyde condensate, fatty alcohol polyoxyethylene ether sulfate, naphthalene sulfonate formaldehyde condensate, sodium naphthalene sulfonate formaldehyde condensate, nonylphenol polyoxyethylene ether, polyoxyethylene lanolin alcohol, alkylphenol polyoxyethylene ether formaldehyde condensate, fatty alcohol polyoxyethylene ether, fatty alcohol polyoxyethylene ether phosphate, polyoxyethylene sorbitan fatty acid ester, and phosphate ester.

[0033] According to an embodiment of the present invention, the wetting agent may be selected from at least one of the following: trisiloxane polyoxyethylene ether, sodium N-lauroyl glutamate, sodium dodecyl sulfate, sodium lauroyl sarcosinate, sodium methylnaphthalene sulfonate formaldehyde condensate, castor oil polyoxyethylene ether, triphenylethylphenol polyoxyethylene ether, sodium dodecylbenzene sulfonate, sodium alkylnaphthalene sulfonate, sodium isooctanol succinate sulfonate, polyoxyethylene alkyl aryl ether, fatty alcohol polyether glycerol fatty acid polyoxyethylene ether, and fatty alcohol polyoxyethylene ether.

[0034] According to an embodiment of the present invention, the adhesive may be selected from at least one of the following: xanthan gum, starch, urea-formaldehyde resin, gelatin, gum arabic, carboxymethyl cellulose, carboxyethyl cellulose, and polyvinyl alcohol.

[0035] According to an embodiment of the present invention, the disintegrant may be selected from at least one of the following: sodium bicarbonate, ammonium sulfate, sodium sulfate, calcium sulfate, and magnesium chloride.

[0036] According to an embodiment of the present invention, the thickener may be selected from at least one of the following: magnesium aluminum silicate, polyvinyl acetate, xanthan gum, gelatin, gum arabic, and polyvinyl alcohol.

[0037] According to an embodiment of the present invention, the defoamer may be selected from at least one of the following: silicone oil, n-octanol, silicone, butyl phosphate, and isobutyl phosphate.

[0038] According to an embodiment of the present invention, the antifreeze agent may be selected from at least one of the following: propylene glycol, ethylene glycol, and glycerol.

[0039] According to an embodiment of the present invention, the stabilizer may be selected from at least one of the following: triethanolamine, epichlorohydrin, butyl glycidyl ether, triphenyl phosphite, N-soybean oil-based trimethylenediamine, and dialkyl succinate acesulfonate.

[0040] According to an embodiment of the present invention, the filler includes solid filler and liquid filler, wherein the solid filler may be selected from at least one of the following: kaolin, attapulgite, diatomaceous earth, silica, bentonite, montmorillonite, calcium carbonate, and talc. The liquid filler may be selected from one or more of soybean oil, castor oil, and mineral oil.

[0041] According to an embodiment of the present invention, the organic solvent may be at least one of the following: ethyl acetate, acetone, isopropanol, 2,2,2-trifluoroethanol, propylene carbonate, benzene, toluene, xylene, dimethylformamide, dimethyl sulfoxide, dichloromethane, cyclohexane, cyclohexanone, N-methylpyrrolidone, or solvent oil (such as 150# solvent oil).

[0042] The present invention also provides the use of the above composition for the prevention and control of pests in agriculture or non-agriculture.

[0043] According to an embodiment of the present invention, the pests are selected from insects such as Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, and mites.

[0044] As one implementation, the pest is selected from the two-spotted spider mite.

[0045] The present invention also provides a method for controlling pests in agriculture or non-agriculture, comprising applying the above composition to plants infested with pests.

[0046] The compositions of this invention can be provided as finished formulations or as single agents. They are mixed directly before use, then diluted with water to achieve the desired concentration. They can be applied to crops or plants in any manner, such as spraying, root irrigation, or application. In specific applications, they can also be mixed with other agents such as growth regulators, soil conditioners, herbicides, and nematicides.

[0047] The present invention also provides a composition for controlling invertebrate pests, comprising a biologically effective amount of compound I, compound II and at least one other component selected from surfactants, solid diluents and liquid diluents, wherein the composition optionally further contains an effective amount of at least one other biologically active compound or activator, wherein compound II is selected from flufenoxuron, KII-9396 or a combination thereof.

[0048] The present invention also provides a method for controlling invertebrate pests, comprising contacting the invertebrate pest or its environment with a biologically effective amount of the composition of the present invention as described above.

[0049] The present invention also provides a spray composition comprising the composition and propellant of the present invention as described above.

[0050] The present invention also provides a bait composition comprising the composition of the present invention as described above; one or more food materials; optional insect attractant; and optional wetting agent.

[0051] The beneficial effects of this invention are: The composition of the present invention has the following advantages: 1) It has a synergistic effect, which can improve the pest control effect; 2) It broadens the insecticidal spectrum, and since pests often occur in the field in a mixed manner, it has a stronger effect on pests; 3) The two active ingredients have different mechanisms of action, and their mixed use can delay the development of pesticide resistance in pests; 4) It reduces the amount of pesticide applied, the number of applications, and the cost of use. Detailed Implementation

[0052] The technical solution of the present invention will be further described in detail below with reference to specific embodiments. It should be understood that the following embodiments are merely illustrative and explanatory of the present invention, and should not be construed as limiting the scope of protection of the present invention. All technologies implemented based on the above content of the present invention are covered within the scope of protection intended by the present invention.

[0053] Unless otherwise stated, the raw materials and reagents used in the following examples are commercially available products or can be prepared by known methods.

[0054] Compound I used in the following examples was prepared by the method described in Example 1 of patent document CN 111909143 A.

[0055] Unless otherwise specified, all percentages in the formulations described in the following examples refer to mass percentages.

[0056] This invention employs a combination of indoor bioassays and field trials to test the insecticidal efficacy of the composition. It should be noted that any modifications, equivalent substitutions, and improvements made within the spirit and principles of this invention should be included within the scope of protection of this invention.

[0057] I. Example of formulation preparation: Preparation Example 1: Wettable Powder Compound I 1~80% Compound II 1~80% Sodium dodecylbenzenesulfonate (wetting agent) 0~8% Sodium lignosulfonate (dispersant) 0~10% Talc (filler) 0~5% Silica (filler) 0~5% Attapulgite soil (fill material) replenished to 100%. The above materials are coarsely pulverized in proportion and then mixed evenly in a mixer. After being pulverized by airflow, the finished product is obtained.

[0058] Preparation Example 2: Water-dispersible granules Compound I 1~80% Compound II 1~80% Sodium alkylnaphthalene sulfonate (wetting agent) 0~8% Sodium lignosulfonate (dispersant) 0~10% Sodium dodecyl sulfate (wetting agent) 0~8% Ammonium sulfate (disintegrant) 0~20% Kaolin (fill material) replenished to 100%. The active ingredients and various additives are mixed evenly according to the formula, and then the powder is obtained by air jet milling. A certain amount of water is added, the mixture is extruded and granulated, and then dried and sieved to obtain the finished product.

[0059] Preparation Example 3: Suspension Compound I 1~50% Compound II 1~50% Sodium methylnaphthalenesulfonate formaldehyde condensate (wetting agent) 0~8% Xanthan gum (thickener) 0~0.3% Sodium salt of naphthalenesulfonic acid formaldehyde condensate (dispersant) 0~5% Propylene glycol (antifreeze) 0~5% Replenish deionized water to 100%. The active ingredients and various additives are mixed evenly according to the formula, and then the mixture is subjected to high-speed shearing and sand milling to obtain the finished product.

[0060] Preparation Example 4: Emulsifiable Oil Compound I 1~50% Compound II 1~50% Tristyrene-phenylphenol polyoxyethylene ether phosphate triethanolamine salt (emulsifier) ​​0~7% Fatty alcohol polyvinyl ether (emulsifier) ​​0~3% Propylene glycol (antifreeze) 0~5% Triethanolamine (stabilizer) 0~0.5% Solvent oil (organic solvent) replenished to 100%. The above raw materials are mixed in proportion to dissolve them into a homogeneous oil phase; after passing inspection, they are metered and packaged to obtain the finished product.

[0061] Preparation Example 5: Water-based emulsion Compound I 1~50% Compound II 1~50% 2,2,2-Trifluoroethanol (organic solvent) 0~35% Propylene carbonate (organic solvent) 0~30% 150# Solvent Oil (Organic Solvent) 0~30% Span-80 (emulsifier) ​​0~10% Ethylene glycol (antifreeze) 0~5% Silicone oil (defoamer) 0~0.2% Polyvinyl alcohol (thickener) 0~0.5% Replenish deionized water to 100%. According to the formula requirements, the above raw materials are added to the mixing tank and mixed evenly by a high-speed shearing machine to make a water-emulsion. After passing the inspection, it is metered and packaged to become the finished product.

[0062] Preparation Example 6: Emulsifiable Oil Compound I 1~50% Compound II 1~50% Tristyrene-phenylphenol polyoxyethylene ether phosphate triethanolamine salt (emulsifier) ​​0~6% Fatty alcohol polyvinyl ether (emulsifier) ​​0~6% Triethanolamine (stabilizer) 0~0.5% Solvent oil (organic solvent) replenished to 100%. The above raw materials are mixed in proportion to dissolve them into a homogeneous oil phase; after passing inspection, they are metered and packaged to obtain the finished product.

[0063] Preparation Example 7: Granules Compound I 0.1~10% Compound II 0.1~10% Sodium lignosulfonate (dispersant) 0~5% Sodium dodecyl sulfate (wetting agent) 0~5% Polyvinyl alcohol (adhesive) 0~3% Silica (filler) 0~5% Diatomaceous earth (filler) should be replenished to 100%.

[0064] Mix all components thoroughly according to the formula ratio, crush them, moisten them with water, stir them thoroughly again, granulate them using a screw extruder, dry them, and then sieve them to obtain the finished product.

[0065] In the above preparation example, compound II is flufenoxuron or KII-9396.

[0066] II. Examples of Indoor Toxicity Testing (1) Tested pests: Two-spotted spider mite ( Tetranychusurticae Koch).

[0067] (2) Measurement method: Methods for determining the toxicity of pests The toxicity of pests was determined using the two-spotted spider mite as the test material, and the toxicity of compound I, compound II and their mixtures was determined.

[0068] Referring to the People's Republic of China Agricultural Industry Standard NY / T 1154.12-2008 "Guidelines for Indoor Bioassay Testing of Pesticides," Part 12: Tetranychus Slide Immersion Method for Insecticides, the test agents (including Compound I and Compound II) were first prepared into five concentration gradients using suitable solvents (the type of solvent, such as acetone, methanol, N,N-dimethylformamide, and dimethyl sulfoxide, etc., selected according to their solubility in the sample). 50 (The concentration gradient is set according to the ratio or arithmetic progression), and the compounds I and II to be mixed are respectively calculated according to their LC values. 50 The values ​​are set according to a series of proportions based on mass ratio, and the final mass concentration (referring to the total mass of compound I and compound II) is prepared by different ratios.

[0069] Cut double-sided tape into 2cm lengths and attach them to one end of a glass slide. Then, select healthy female adult mites and attach their backs to the double-sided tape (be careful not to stick the mite legs, antennae, or mouthparts). Place 30 mites per slide and put them in a container lined with a damp sponge. Cover the container and place it at (25±1)℃. After 2 hours, examine the slides under a microscope, remove any dead or injured individuals, and replenish the slides to 30 mites per slide. Immerse the slides in the solution and gently shake for 5 seconds. Remove the slides, absorb excess solution with absorbent paper, and place them in a white porcelain dish lined with a damp sponge. Cover with a translucent plastic film. Each treatment was repeated four times, with a blank control set up for a treatment without the reagent (containing organic solvents and emulsifiers).

[0070] According to Sun Yunpei's method, the co-toxicity coefficient (CTC) of two drugs with different ratios is calculated. A CTC ≥ 120 indicates a synergistic effect, a CTC ≤ 80 indicates an antagonistic effect, and 80 < CTC < 120 indicates an additive effect. The CTC of the mixture is calculated according to formulas (1), (2), and (3): (1) In the formula: ATI – Actual Measured Toxicity Index of Mixtures; S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L); M – LC of the mixture 50 The unit is milligrams per liter (mg / L). (2) In the formula: TTI – Theoretical Co-toxicity Index of Mixtures; TI A —A. Toxicity index of drug A; P A —The percentage content of drug A in the mixture, expressed as a percentage (%). TI B —Toxicity index of drug B; P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0071] (3) In the formula: CTC – Cotoxicity Coefficient; ATI – Actual Toxicity Index of Mixtures; TTI – Theoretical Toxicity Index of Mixtures.

[0072] When different pesticides are mixed, they typically exhibit three types of effects: additive, synergistic, and antagonistic. However, the specific type of effect cannot be predicted. The composition described in this invention uses compounds I and II as active ingredients, and bioassay examples are used to illustrate this.

[0073] Indoor pesticide activity assay for two-spotted spider mite This experiment used the mite slide immersion method to conduct an indoor activity test of the compound against the two-spotted spider mite, in order to determine the activity of the insecticidal compositions of Examples 1-2 against the two-spotted spider mite.

[0074] Example 1: To verify the effects of different ratios of Compound I and flufenoxuron on Tetranychus tinctoria, indoor toxicity tests were conducted on Tetranychus tinctoria using different mass ratios of Compound I and flufenoxuron. The corresponding concentrations were prepared according to the mass ratios in the table, and indoor insecticidal experiments were performed. The results are as follows: Table 1. Results of toxicity assays of Compound I and single agents and mixtures of flufenoxuron against Tetranychus spp.

[0075] As shown in Table 1, different ratios of compound I and flufenoxuron significantly enhanced the activity of Tetranychus bicolor.

[0076] Example 2: To verify the efficacy of different dosage ratios of compound I and KII-9396 against the two-spotted spider mite, indoor toxicity tests were conducted on different mass ratios of compound I and KII-9396 against the two-spotted spider mite. The corresponding concentrations were prepared according to the mass ratios listed in the table, and indoor insecticidal experiments were performed. The results are as follows: Table 2. Results of toxicity assays of Compound I and KII-9396, alone and in mixture, against Tetranychus spp.

[0077] As shown in Table 2, the combined use of compound I and KII-9396 at different ratios significantly enhanced the activity of Tetranychus bicolor.

[0078] The embodiments of the present invention have been described above. However, the present invention is not limited to the above embodiments. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A composition comprising compound I and compound II, characterized in that, The compound II is KII-9396. The structure of compound I is shown below. ， The KII-9396 is (RS)-[5-[4-chloro-2-fluoro-5-[(2,2,2-trifluoroethyl)sulfinyl]phenoxy]pentyl]trifluoromethyl sulfide (CAS: 1472050-04-6).

2. The composition according to claim 1, characterized in that, The mass ratio of compound I to compound II is 80~1:1~80.

3. The composition according to claim 1 or 2, characterized in that, The composition further includes at least one of the following: deionized water, organic solvent, emulsifier, dispersant, wetting agent, thickener, defoamer, stabilizer, binder, disintegrant, antifreeze, anticaking agent, suspending agent, film-forming agent, preservative, colorant, polymeric capsule wall material, pH adjuster, or filler.

4. The composition according to claim 1, characterized in that, The composition can be formulated into various dosage forms, including wettable powders, water-dispersible granules, suspensions, water-in-oil emulsions, suspension seed coating agents, microcapsule suspensions, microcapsule suspension-suspension agents, emulsifiable concentrates, microemulsions, dispersible liquids, or granules.

5. Use of the composition according to any one of claims 1-4 for the prevention and control of pests in agriculture or non-agriculture.

6. The use according to claim 5, characterized in that, The pests are selected from Lepidoptera, Hemiptera, Coleoptera, Thysanoptera, Diptera, and mites.

7. A method for controlling pests in agricultural or non-agricultural fields, characterized in that, This includes applying the composition of any one of claims 1-4 to plants infested with pests.

8. A composition for controlling invertebrate pests, characterized in that, It comprises a biologically effective amount of compound I, compound II and at least one other component selected from surfactants, solid diluents and liquid diluents, the composition optionally further containing an effective amount of at least one other biologically active compound or activator, wherein compound II is selected from KII-9396; The structure of compound I is shown below. 。 9. A method for controlling invertebrate pests, characterized in that, This includes contacting invertebrate pests or their environment with a biologically effective amount of the composition according to any one of claims 1-4.

10. A spray composition comprising the composition according to any one of claims 1-4 and a propellant; or, A bait composition comprising the composition according to any one of claims 1-4; one or more food materials; optional insect attractant; and optional wetting agent.

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