A pesticide composition and use thereof

Abstract

The present application belongs to the technical field of pesticide insecticide, and relates to a kind of pesticide composition and its application.The effective component of the pesticide composition includes active ingredient A and active ingredient B, the active ingredient A is a compound of formula (I), the active ingredient B is Benzpyrimoxan or Oxazosulfyl, and the mass ratio of the active ingredient A to the active ingredient B is 1:42-32:1.The composition of the present application has the advantages of synergism and resistance delay, and is used for preventing and treating hemipterous pests.

Description

Technical Field

[0001] This invention belongs to the field of pesticide technology, and specifically relates to a pesticide composition. Background Technology

[0002] Benzpyrimoxan is a novel insecticide developed by Nippon Nippon Kogyo Co., Ltd. Its chemical name is 5-(1,3-dioxane-2-yl)pyrimidin-4-yl-4-(trifluoromethyl)benzyl ether, and its structural formula is as follows:

[0003]

[0004] Oxazosulfyl is a novel benzoxazole insecticide developed by Sumitomo Chemical Co., Ltd. of Japan. Its chemical name is 2-[3-(ethylsulfonyl)-2-pyridyl]-5-[trifluoromethyl(sulfonyl)]benzoxazole, and its structural formula is as follows:

[0005]

[0006] In agricultural production, the control of various harmful insects mainly relies on the application of large quantities of pesticides. The excessive, frequent, and irrational use of pesticides leads to increasing resistance in hemipterans, causing more severe damage to crops and making control increasingly difficult. Mixing different pesticides is an effective way to delay insect resistance, but the effects of these mixtures vary. Therefore, researching and developing highly efficient, low-toxicity, and environmentally friendly insecticide compositions is of great significance for the sustainable development of agriculture. By mixing compound (I) with two or more agents with different mechanisms of action, such as Benzpyrimoxan and Oxazosulfyl, it is possible to effectively control hemipteran pests and reduce the development of insecticide resistance. Summary of the Invention

[0007] The technical problem to be solved by the present invention is to overcome the shortcomings of the prior art, and to propose a pesticide composition with synergistic effect and resistance reduction for hemiptera pests, and its application.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows: a pesticide composition comprising active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I). The active ingredient B is Benzpyrimoxan or Oxazosulfyl;

[0009] Furthermore, the mass ratio between active ingredient A and active ingredient B is 1:42 to 32:1;

[0010] Furthermore, the mass ratio of the compound of formula (I) to Benzpyrimoxan is 1:35 to 32:1;

[0011] Furthermore, the mass ratio of the compound of formula (I) to Benzpyrimoxan is 1:35 to 16:1 or any value between the above values;

[0012] Furthermore, the mass ratio of the compound of formula (I) to Benzpyrimoxan is 1:35, 1:20, 1:18, 1:10, 1:8, 1:5, 1:4, 1:2, 1:1, 4:1, 5:1, 15:1, 16:1, or 32:1;

[0013] Furthermore, the mass ratio of the compound of formula (I) to Oxazosulfyl is 1:42 to 25:1;

[0014] Furthermore, the mass ratio of the compound of formula (I) to Oxazosulfyl is 1:20 to 25:1 or any value between the above values;

[0015] Furthermore, the mass ratio of the compound of formula (I) to Oxazosulfyl is 1:42, 1:20, 1:15, 1:8, 1:6, 1:3, 1:2, 3:2, 3:1, 4:1, 12:1, 15:1, or 25:1;

[0016] Furthermore, based on a total mass of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 0.5% to 90%.

[0017] Furthermore, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 1% to 85%;

[0018] Furthermore, in addition to the active ingredient, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

[0019] Furthermore, the pesticide composition further includes an adjuvant selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists, and carriers;

[0020] The wetting agent is selected from one or more of the following: alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl succinate sulfonate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, soapberry powder, soapberry powder, SOPA, detergents, emulsifiers 2000 series, and wetting and penetrating agents F; and / or

[0021] The dispersant is selected from one or more of the following: lignin sulfonates, alkyl naphthalene sulfonates formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensates sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0022] The emulsifier is selected from one or more of the following: calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrene-phenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0023] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and silica; and / or

[0024] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0025] Antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0026] Defoamer selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of the fatty alcohols; and / or

[0027] The solvent is selected from one or more of benzene, toluene, xylene, mesitylene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, hydrocarbon carbonates, diesel oil, solvent oil, vegetable oil, vegetable oil derivatives, and water; and / or

[0028] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon, and 1,2-benzisothiazolin-3-one; and / or

[0029] The stabilizer is selected from one or more of the following: disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, silica, talc, montmorillonite, and starch; and / or

[0030] Synergists are selected from synergistic phosphorus, synergistic ether; and / or

[0031] The carrier is selected from one or more of the following: ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils, and vegetable oil derivatives.

[0032] Furthermore, the pesticide composition can be prepared into an agriculturally permissible formulation, wherein the formulation is selected from solid and / or liquid formulations;

[0033] Furthermore, the solid dosage forms include powders, granules, balls, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets, or soluble granules;

[0034] Furthermore, the liquid formulation includes soluble agents, colloids, oils, spreading oils, emulsions, latexes, dispersible liquids, ointments, water emulsions, oil emulsions, microemulsions, lipids, suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspensions, microcapsule suspension-suspensions, microcapsule suspension-water emulsions, or microcapsule suspension-suspension emulsions;

[0035] Furthermore, the solid dosage form is selected from wettable powders and water-dispersible granules; the liquid dosage form is selected from emulsifiable concentrates, water-in-oil emulsions, microemulsions, suspensions, suspension emulsions, and dispersible oil suspensions.

[0036] The application of the pesticide composition described in this invention in the control of agricultural and forestry pests and sanitary pests;

[0037] The pesticide composition described in this invention can be used to control pests on fruit trees, vegetables, ornamental plants, tea, cotton, and cereal crops.

[0038] Furthermore, the aforementioned agricultural and forestry pests and sanitary pests are pests belonging to the orders Hemiptera, Thysanoptera, Lepidoptera, and Diptera.

[0039] Furthermore, the aforementioned Hemiptera pests include: green stink bugs (Acrosternum hilare), chinch bugs (Blissus leucopterus), potato blind bugs (Calocoris norvegicus), aphids, scales, whiteflies, leafhoppers, pea aphids (Acrythosiphon pisum), Adelges spp., cabbage whiteflies (Aleurodes proletella), and spiral whiteflies (Aleurodicus). The following species are listed: * *disperses*, *Aleurothrixus floccosus* (woolly whitefly), *Aluacaspis spp.*, *Aonidiella aurantii*, *Aphis spp.*, *Aphis gossypii* (cotton aphid), *Aphis pomi*, *Aulacorthum solani* (foxglove aphid), *Bemisia spp.* (whitefly), *Bemisia argentifolii*, *Bemisia tabaci* (sweet potato whitefly), *Brachycolus noxius*, *Brachycorynella asparagi* (asparagus aphid), *Brevennia rehi*, and *Brevicoryne*. Species of the genera *Ceroplastes* (brassicae), *Ceroplastes spp.*, *Ceroplastes rubens* (red wax scale), *Chionaspis* (spp.), *Chrysomphalus* (spp.), *Dysaphis plantaginea* (rosy apple aphid), and *Empoasca* (green leafhopper).Apple cotton aphid (Eriosomalanigerum), cottony cushion scale (Icerya purchasi), mango leafhopper (Idioscopus nitidulus), planthopper (Laodelphaxstriatellus), oyster scale (Lepidosaphes spp.), long-tubed aphid (Macrosiphum spp.), euphorbiae, granarium, rosae, quadrilineatus (aster leafhopper), frimbiolata, dirhodum, longicornis, myzus spp., persicae (green peach aphid) The following species are listed: *Nephotettix* spp., *Nephotettix cinctipes* (green leafhopper), *Nilaparvata lugens*, *Parlatoria pergandii*, *Peregrinus maidis* (corndelphacid), *Philaenus* spp., *Phylloxeravitifoliae* (grape phylloxera), *Physokermes piceae* (spruce bud scale), *Planococcus* spp. (mealyptus), *Pseudococcus* spp. (mealyptus), and *Pseudococcus brevipes* (pineapple mealybug). The species *Mealybug*, *Quadraspidiotus perniciosus* (San Josescale), and *Rhopalosiphum spp.*The following aphids are listed: * *Rhopalosiphum maida* (corn leaf aphid), * *Rhapalosiphum padi* (oat bird-cherry aphid), * *Saissetia spp.*, * *Saissetia oleae*, * *Schizaphis graminum* (green bug), * *Sitobion avenae* (English grain aphid), * *Sogatella furcifera*, * *Therioaphis spp.*, * *Toumeyella spp.*, * *Trialeurodes spp.*, and * *Trialeurodes vaporariorum*.

[0040] Furthermore, the aforementioned Thysanoptera pests include: *Thrips palmi* Karny, *Thrips tabaci*, *Frankliniella fusca* (tobaccothrips), *Frankliniella occidentalis* (western flowerthrips), *Frankliniella shultzei*, *Frankliniella williamsi* (cornthrips), *Heliothrips haemorrhaidalis* (greenhouse thrips), *Riphiphorothrips cruentatus*, *Scirtothrips* spp., and *Scirtothrips citri* (citrus thrips). Thrips, yellow tea thrips (Scirtothripsdorsalis), Taeniothrips rhopalantennalis, and thrips spp.

[0041] Furthermore, the aforementioned Lepidoptera pests include: *Adoxophyes spp.*, *Adoxophyes orana*, *Agrotis spp.* (root cutter), *Agrotis ipsilon* (black cutworm), *Alabamaargillacea* (cotton leafworm), *Amorbia cuneana*, *Amyelosis transitella* (navel orange moth), *Anacamptodes defectaria*, *Anarsia lineatella* (peach twig borer), *Anomis sabulifera* (jute looper), *Anticarsia gemma ta lis*, *Archips argyrospila* (fruittree leafroller), and *Archips* (rose leafroller). *Argyrotaenia spp.* (rose leaf roller), *Argyrotaenia citrana* (orange tortrix), *Autographa gamma*, *Bonagota cranaodes*, *Borbo cinnara* (rice leaf folder), *Bucculatrix thurberiella* (cotton leafperforator), *Caloptilia spp.* (leaf miners), *Ca pua reticulana*, *Carposina niponensis* (peach fruit moth), *Chilo spp.*, *Chlumetia transversa* (mango shoot borer), *Choristoneurarosaceana* (obliquebanded rose leaf roller) Leafroller), and species of the genus Chrysodeixis spp.The following species are listed: *Cnaphalocerus medinalis* (grass leafroller), *Colias* spp., *Conpomorpha cramerella*, *Cossus cossus* (carpenter moth), *Crambus* spp. (soil webworms), *Cydiafunebrana* (plum fruit moth), *Cydia molesta* (oriental fruit moth), *Cydia nignicana* (pea moth), *Cydiapomonella* (codling moth), *Darna diducta*, and *Diaphania* spp. (stem borer). *Diatraea* spp. (stalk borers), *Diatraeas accharalis* (sugarcane borer), *Diatraea graniosella* (southwester corn borer), *Earias* spp. (cotton bollworm), *Earias insulata* (Egyptian cotton bollworm), *Earias vitella* (rough northern bollworm), *Ecdytopopha aurantianum*, *Elasmopalpus lignosellus* (lesser cornstalk borer), *Epiphysias postruttana* (light brown apple moth), *Ephestia* spp. (flour moths), *Ephestia* cautella (almond moth), Tobacco moth (Ephestia elutella), Mediterranean flour moth (Ephestia kuehniella), and species of the genus Epimeces (Epimeces spp.).), Epinotia aporema, Erionotathrax (banana skipper), Eupoecilia ambiguella (grape leafroller), Euxoa auxiliaris (army cutworm), Feltia spp. (root cutter), Gortyna spp. (stemborers), Grapholitamolesta (oriental fruit borer).

[0042] (oriental fruit moth)), Hedylepta indicate (bean leaf webber), Helicoverpa sp p. (noctus moth), Helicoverpa armigera, Helicoverpa zea, Heliothis spp., Heliothis virescens, Hellula undalis (cabbage webworm), Indarbela spp. (root borers), Keiferia lycopersicella, Leucinodes orbonalis, Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (grape fruit moth) The following species are listed: fruitmoth, Loxagrotis spp., Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), and Malacosoma spp.(tent caterpillars), cabbage armyworm (Mamestra brassicae), bean pod borer (Maruca testulalis), bagworm (Metisa plana), true armyworm (Mythimna unipuncta), elegantalis (Neoleucinodes elegantalis), depunctalis (Nymphula depunctalis), winter inchworm (Operophtherabrumata), European corn borer (Ostrinia nubilalis), Oxydiavesulia, common currant tortrix (Pandemis cerasana), brown apple tortrix (Pandemis heparana), African swallowtail butterfly (Papilio demodocus), pink bollworm (Pectinophora gossypiella). The following species are listed: bollworm, Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phylloocnisitis citrella (citrus leafminer), Phyllonorycter spp. (leek moth), Pieris rapae (cabbage white butterfly), Plathapena scabra (alfalfa green armyworm), Plodia interpunctella (Indian grain moth), Plutellaxylostella (diamondback moth), Polychrosis viteana (grape leafroller), Prays endocarpa (citrus fruit borer), and Prays endocarpa (olive borer). oleae (olive moth), species of the genus Pseudaletia (Pseudaletia spp.).(Noctus), Spodoptera litura Fabricius, Pseudaletia unipunctata (armyworm), Pseudoplusia includes (soybean noctus), Rachiplusia nu, Chilo suppressalis (Walker), Scirpophaga incertulas, Sesamia spp. (stemborers), Sesamia inferens (pink rice stem borer), Sesamia nonagrioides, Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp., Spodoptera exigua, Spodoptera fugiperda), Southern armyworm (Spodoptera oridania), Synanthedon spp., Thecla basilides, Thermisia gemmatalis, clothes moth (Tineola bisselliella), cabbage white butterfly (Trichoplusia ni), cabbage white butterfly (Pierisrapae Linne), tomato leafminer (Tuta absoluta).

[0043] Furthermore, the hemiptera pests mentioned include whiteflies, rice planthoppers, and aphids;

[0044] The present invention also provides a method of using the pesticide composition as described above, specifically applying it in an effective dose to the pest that needs to be controlled or to the medium in which it grows.

[0045] The pesticide composition of the present invention has the following advantages:

[0046] 1) The pesticide composition of the present invention exhibits a synergistic effect within a certain ratio range, resulting in significant control effects;

[0047] 2) The pesticide composition has two active ingredients with unique mechanisms of action, which delay the development of pest resistance and extend the product's lifespan. Detailed Implementation

[0048] The present invention will be further described below with reference to the embodiments. The percentages in the embodiments are all weight percentages, but the present invention is not limited thereto.

[0049] The compositions of the present invention can be provided in formulation form. They can be formulated as suspensions, water-dispersible granules, wettable powders, etc., as needed. The content of the active ingredient in the compositions of the present invention depends on the application rate when used alone, as well as on the mixing ratio and the degree of synergistic effect. The optimal range of active ingredient content varies depending on the type of formulation of the composition.

[0050] Formulation preparation examples

[0051] Example 1:

[0052] 20% Formula (I) Compound · benzpyrimoxan Suspension (4+16)

[0053] Formula: 4% compound of formula (I), 16% benzpyrimoxa,

[0054] 2.5% fatty alcohol polyoxyethylene ether, 2% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 4% alkylphenol polyoxyethylene ether phosphate salt, 0.8% magnesium aluminum silicate, 0.25% xanthan gum, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, deionized water to make up the balance;

[0055] Preparation method: Add the active ingredient to the wetting and dispersing agent and defoamer, use zirconia beads, and wet pulverize with a sand mill to D. 90 (90% of the particles have a particle size) <10μm to obtain a pulverized slurry. Thickener, antifreeze, and preservative are added to the pulverized slurry and mixed evenly. Deionized water is added to make up to 100%, and the mixture is sheared at high speed to obtain a suspension product.

[0056] Example 2:

[0057] 50% Formula (I) Compound · benzpyrimoxan Water Dispersible Granules (15+35)

[0058] Formula: 15% compound of formula (I), 35% benzpyrimoxa,

[0059] 6% polycarboxylate (Tersperse 2700), 2% dispersant NNO, 6% naphthalene sulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 0.5% ammonium sulfate, 4% sodium sulfate, and kaolin to make up the balance;

[0060] Preparation method: According to the formula ratio, the active ingredients are added to the carrier, and surfactants and other functional additives are added to it. After mixing, the mixture is pulverized by air jet and then an appropriate amount of water is added. The mixture is then kneaded, granulated, dried and sieved to obtain the water-dispersible granule product.

[0061] Example 3:

[0062] 55% Formula (I) Compound · benzpyrimoxan wettable powder (10+45)

[0063] Formula: 10% compound of formula (I), 45% benzpyrimoxa,

[0064] 5% naphthalene sulfonate formaldehyde condensate, 12% tea saponin, 2% sodium dodecyl sulfate, 10% attapulgite, and kaolin to make up the balance;

[0065] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a stirring tank, and then pulverized and mixed evenly multiple times by an air jet mill to prepare the wettable powder of the composition of the present invention.

[0066] Example 4:

[0067] 20% Formula (I) Compound·oxazosulfyl Suspension (5+15)

[0068] Formula: 5% compound of formula (I), 15% oxazosulfyl,

[0069] 3% fatty alcohol polyoxyethylene ether, 2.5% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 1.5% styrene phenol polyoxyethylene ether phosphate, 1% lignosulfonate, 0.8% magnesium aluminum silicate, 0.25% xanthan gum, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone defoamer, deionized water to make up the balance;

[0070] Preparation method: Same as in Example 1.

[0071] Example 5:

[0072] 45% Formula (I) compound oxazosulfyl water-dispersible granules (10+35)

[0073] Formula: 10% compound of formula (I), 35% oxazosulfyl,

[0074] 6% sodium polycarboxylate, 2% dispersant NNO, 6% naphthalene sulfonate formaldehyde condensate, 2% sodium dodecyl sulfate, 0.5% ammonium sulfate, 4% light calcium carbonate, and kaolin to make up the balance;

[0075] Preparation method: Same as in Example 2.

[0076] Example 6:

[0077] 60% Formula (I) Compound · oxazosulfyl wettable powder (12+48)

[0078] Formula: 12% compound of formula (I), 48% oxazosulfyl,

[0079] 5% naphthalene sulfonate formaldehyde condensate, 10% tea saponin, 2% sodium dodecyl sulfate, 5% diatomaceous earth, and kaolin to make up the balance;

[0080] Preparation method: Same as in Example 3.

[0081] Indoor bioactivity assay

[0082] The examples refer to the guidelines for indoor bioassay of pesticides, Part 6: Insect immersion method NY / T 1154.6-2006; Part 7: Determination of the combined effects of mixtures NY / T 1154.7-2006; Part 9: Spraying method NY / T 1154.9-2008; and Part 14: Leaf immersion method NY / T 1154.14-2008, etc.

[0083] Specific indoor toxicity tests:

[0084] 1) Experimental target: rice planthopper

[0085] Test method: spray method;

[0086] Test insects: healthy adult insects with uniform growth and development;

[0087] Reagent preparation: Dissolve the test reagent in acetone and prepare five series of concentrations using a 0.1% Tween 80 aqueous solution.

[0088] Experimental treatment: Select at least 15 test insects with the same physiological state using an insect aspirator and place them in a petri dish. Then place the petri dish in the bottom tray of a potter spray tower for quantitative spraying. The spray volume is 1 mL. After the drug solution settles for 1 minute, remove the test insects. Each treatment is repeated 4 times, with a blank treatment as a control.

[0089] The treated test insects were raised in an artificial intelligence culture room at 25±1℃, with a light duration of L:D = 16h:8h and a relative humidity of 65%±5%.

[0090] Investigation method: The mortality of test insects was investigated 48 hours after the treatment with the agent. The criteria for judging the mortality of test insects were obvious shrinkage of the insect body or inability to crawl normally when punctured. The total number of insects and the number of dead insects were recorded.

[0091] 2) Test target: Aphids

[0092] Experimental method: Insect immersion method;

[0093] Test insects: healthy, uniformly developed second-instar cotton aphid nymphs;

[0094] Reagent preparation: Dissolve the test reagent in acetone and prepare five series of concentrations using a 0.1% Tween 80 aqueous solution.

[0095] Experimental treatment: Second-instar nymphs with consistent physiological condition and raised indoors were selected and immersed in the drug solution for 5 seconds using an insect dipping device. 15 insects were treated per treatment, and the treatment was repeated 4 times. The control group was treated with 0.1% Tween 80 aqueous solution. After treatment, the insects were transferred to 9.0 cm diameter petri dishes lined with filter paper for rearing, and fresh leaves were replaced daily.

[0096] The treated test insects were raised in an artificial intelligence culture room at 25±1℃, with a light duration of L:D = 14h:10h and a relative humidity of 65%±5%.

[0097] Investigation method: The mortality of test insects was investigated 48 hours after the treatment with the agent. The criteria for judging the mortality of test insects were obvious shrinkage of the insect body or inability to crawl normally when punctured. The total number of insects and the number of dead insects were recorded.

[0098] Data statistics and analysis:

[0099] Based on the survey data, calculate the adjusted mortality rate for each treatment. Use the following formula to calculate the mortality rate, and round the results to two decimal places:

[0100]

[0101] In the formula:

[0102] P – Mortality rate, expressed as a percentage (%);

[0103] K represents the number of dead insects, in heads;

[0104] N represents the total number of insects treated, in units of heads.

[0105]

[0106] In the formula:

[0107] P1 – Corrected mortality rate, in percentage (%);

[0108] P t —The mortality rate is expressed as a percentage (%).

[0109] P0 – Mortality rate in the blank control group, expressed as a percentage (%).

[0110] If the control mortality rate is <5%, no correction is needed; if the control mortality rate is between 5% and 20%, correction should be performed according to the formula; if the control mortality rate is >20%, the trial needs to be repeated.

[0111] The data is processed using probability value analysis. A data statistical analysis system can be used to analyze the data and determine the toxicity regression line and LC. 50 The values, their 95% confidence limits, and correlation coefficients r are used to evaluate the activity of the test reagent on the biological sample.

[0112] The co-toxicity coefficient (CTC value) of the mixture is calculated using the following formula:

[0113]

[0114] In the formula:

[0115] ATI – Actual Measured Toxicity Index of Mixtures;

[0116] S – LC50 of standard insecticides 50 The unit is milligrams per liter (mg / L);

[0117] M – LC of the mixture 50 The unit is milligrams per liter (mg / L).

[0118] TTI = TI A *P A +TI B *P B

[0119] In the formula:

[0120] TTI – Theoretical Toxicity Index of Mixtures;

[0121] TI A —A. Toxicity index of drug A;

[0122] P A —Percentage content of drug A in the mixture, expressed as percentage (%);

[0123] TI B —Toxicity index of drug B;

[0124] P B —Percentage content of agent B in the mixture, expressed as percentage (%).

[0125]

[0126] In the formula:

[0127] CTC – Cotoxicity Coefficient;

[0128] ATI – Actual Measured Toxicity Index of Mixtures;

[0129] TTI – Theoretical Toxicity Index of Mixtures.

[0130] The co-toxicity coefficient of the compound is ≥120, which shows a synergistic effect; CTC≤80 shows an antagonistic effect; and 80<CTC<120 shows an additive effect.

[0131] Experimental results:

[0132] Co-toxicity of the mixed compound against rice planthopper: The test results showed that the LC50 of compound (I) against rice planthopper was... 50 The concentration was 4.702 mg / L. Compound (I) and benzpyrimoxan or oazosulfyl showed good synergistic effects when mixed with rice planthopper.

[0133] Table 1 shows that when the mass ratio of compound (Ⅰ) to benzpyrimoxan is 1:35 to 32:1, the co-toxicity coefficient of the control efficacy against rice planthopper is greater than 80, showing an additive or synergistic effect. When the mass ratio is 1:35 to 16:1, the co-toxicity coefficient of the control efficacy against rice planthopper is greater than 120, showing a synergistic effect.

[0134] Table 1 shows the toxicity test results of compound (I) and benzpyrimoxan in different ratios against rice planthoppers.

[0135]

[0136] The results in Table 2 show that when the mass ratio of compound (I) to oazosulfyl is 1:42 to 25:1, the co-toxicity coefficient against rice planthopper is greater than 80, indicating an additive or synergistic effect. When the mass ratio of compound (I) to oazosulfyl is 1:15 to 25:1, the co-toxicity coefficient is greater than 120, indicating a synergistic effect.

[0137] Table 2 shows the toxicity test results of compound (I) and oxazosulfyl in different ratios against rice planthoppers.

[0138]

[0139] Co-toxicity of the mixed compound against cotton aphids: Experimental results showed that the LC50 of compound (I) against cotton aphids was... 50 The concentration was 84.113 mg / L. Compound (I) and its combination with benzpyrimoxan or oazosulfyl showed good synergistic effects against cotton aphids.

[0140] Table 3 shows that when the mass ratio of compound (Ⅰ) to benzpyrimoxan is 1:18 to 15:1, the co-toxicity coefficient of the control efficacy against cotton aphids is greater than 120, indicating a synergistic effect.

[0141] Table 3 shows the toxicity test results of compound (I) and benzpyrimoxan in different ratios against cotton aphids.

[0142]

[0143]

[0144] Table 4 shows that when the mass ratio of compound (Ⅰ) to oxazosulfyl is 1:20 to 12:1, the co-toxicity coefficient of the control efficacy against cotton aphids is greater than 120, indicating a synergistic effect.

[0145] Table 4 shows the toxicity test results of compound (I) and oxazosulfyl in different ratios against cotton aphids.

[0146]

[0147] Field efficacy examples

[0148] Field Trial 1

[0149] Experimental crops and targets: rice (Xin Dao 18), rice planthopper;

[0150] Experiment location: Cotton planting base in Tongshi Town, Linyi City, Shandong Province;

[0151] Application equipment: 3WBS16 backpack electric sprayer. First, spray the control plot with water, then spray the experimental pesticide plot. When spraying, point the nozzle downwards and spray the pesticide solution as close to the base of the rice stem as possible.

[0152] Test date: May 16, 2023;

[0153] Experimental environment: The cultivation conditions of all experimental plots were uniform and the same level of fertilizer and water management was used. The rice grew well and no agricultural operations such as fertilization and irrigation were carried out during the experiment.

[0154] Test reagents:

[0155] Table 5 Field Trial Design and Pesticide Dosage

[0156]

[0157]

[0158] Cell size and duplication: randomized block arrangement, 25m per cell 2 Each treatment was repeated 4 times.

[0159] Survey method: Five random sampling points were taken in each plot, with two clumps at each point. A 20×33cm white porcelain plate was used as the carrier, with a small amount of water in the plate. The rice clumps were tapped 4 times each time, and the number of planthoppers in the plate was counted. The number of residual insects in each plot was investigated 3 days and 10 days after the treatment.

[0160] Calculation formulas and data analysis:

[0161]

[0162] Results of field efficacy trials:

[0163] The field control effects are shown in Table 6. Three days after application, the 20% (I) compound·benzpyrimoxan suspension (4+16) and the 20% (I) compound·oxazosulfyl suspension (5+15) showed good rapid efficacy against rice planthoppers in paddy fields, with control efficiencies of 83.14% and 85.36%, respectively. Ten days after application, the mixed formulations also showed good residual efficacy, with control efficiencies above 90%, significantly higher than their control single agents.

[0164] Table 6. Field control efficacy of different pesticides against rice planthoppers.

[0165]

[0166] Note: The above efficacy data are the average of 4 repetitions, and the values ​​are rounded to two decimal places.

[0167] Safety: Observations showed that the experimental pesticide treatment did not cause phytotoxicity or other adverse effects on rice compared with the blank control area.

[0168] Field Trial 2

[0169] Experimental crops and targets: cotton (Lu Mian 338), aphids;

[0170] Experimental location: Vegetable greenhouse in Qingzhou, Weifang City, Shandong Province;

[0171] Application equipment: 3WBS16 backpack electric sprayer;

[0172] Test date: June 15, 2022;

[0173] Experimental environment: The cultivation conditions in all experimental plots were uniform and the same level of fertilizer and water management was used;

[0174] Test reagents:

[0175] Table 7 Field Trial Design and Pesticide Dosage

[0176]

[0177] Experimental method: Apply pesticide once during the peak period of aphid infestation.

[0178] Cell size and duplication: randomized block arrangement, 20m per cell 2 Each treatment was repeated 4 times.

[0179] Survey method: Five random sampling points were taken in each plot, with one plant fixed at each point. The number of aphids on all leaves of the whole plant was investigated. The initial insect population was investigated before the pesticide was applied, and the number of residual insects was investigated 3 days and 7 days after the pesticide was applied.

[0180] Calculation formulas and data analysis:

[0181]

[0182] Results of field efficacy trials:

[0183] Table 8 shows the field control efficacy of different pesticides against cotton aphids. As can be seen from the table, 3 days after application, 55% compound (I)·benzpyrimoxan wettable powder (10+45) and 60% compound (I)·oxazosulfyl wettable powder (12+48) showed good rapid efficacy, with control efficiencies of 81.33% and 80.27%, respectively. 7 days after application, the mixed formulations also showed good residual efficacy, with control efficiencies of 92.05% and 91.72%, respectively, significantly higher than their control single agents.

[0184] Table 8. Field control efficacy of different pesticides against cotton aphids.

[0185]

[0186] Note: The above efficacy data are the average of 4 repetitions, and the values ​​are rounded to two decimal places.

[0187] Safety: During and after the trial period, the cotton grew normally and no obvious pesticide damage was observed.

[0188] The pesticide compositions or formulations obtained by this invention exhibit significant preventative efficacy, demonstrating superior performance compared to single-agent formulations in delaying the development of resistance and prolonging pesticide retention. Furthermore, no phytotoxicity was observed in the experiments with the compounded pesticides, indicating that the enhanced synergistic insecticidal effect of the resulting pesticide compositions or formulations can reduce production and usage costs while ensuring crop safety.

[0189] Although the present invention has been described in detail above with general description and specific embodiments, some modifications or improvements can be made to it based on the present invention, which will be obvious to those skilled in the art. Therefore, all such modifications or improvements made without departing from the spirit of the present invention are within the scope of protection claimed by the present invention.

Claims

1. A pesticide composition, characterized in that: It includes active ingredient A and active ingredient B, wherein active ingredient A is a compound of formula (I). The active ingredient B is either Benzpyrimoxan or Oxazosulfyl.

2. The pesticide composition according to claim 1, characterized in that: The mass ratio between active ingredient A and active ingredient B is 1:42 to 32:

1.

3. The pesticide composition according to claim 1, characterized in that: The mass ratio of the compound of formula (I) to Benzpyrimoxan is 1:35 to 32:1; The mass ratio of the compound of formula (Ⅰ) to Oxazosulfyl is 1:42 to 25:

1.

4. The pesticide composition according to claim 1, characterized in that: The mass ratio of the compound of formula (I) to Benzpyrimoxan is 1:35 to 16:1; The mass ratio of the compound of formula (Ⅰ) to Oxazosulfyl is 1:20 to 25:

1.

5. The pesticide composition according to claim 1, characterized in that: Based on a total mass of 100 wt% of the pesticide composition, the sum of the contents of active ingredient A and active ingredient B in the pesticide composition is 0.5% to 90%.

6. The pesticide composition according to claim 1, characterized in that: In addition to the active ingredient, the pesticide composition also includes agriculturally acceptable auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoamers, solvents, preservatives, stabilizers, synergists or carriers.

7. The pesticide composition according to claim 6, characterized in that: The pesticide composition can be prepared into an agriculturally permissible formulation, wherein the formulation is selected from solid and / or liquid formulations; Preferably, the solid formulation is selected from wettable powders and water-dispersible granules; the liquid formulation is selected from emulsifiable concentrates, water-emulsions, microemulsions, suspensions, suspension emulsions, and dispersible oil suspensions.

8. The application of the pesticide composition according to any one of claims 1-7 in the control of agricultural and forestry pests and sanitary pests.

9. The application according to claim 8, characterized in that: The agricultural and forestry pests and sanitary pests mentioned are pests belonging to the orders Hemiptera, Thysanoptera, and Lepidoptera. Preferably, the agricultural and forestry pests and sanitary pests are hemiptera pests.

10. The pesticide composition according to claim 1, characterized in that: The pesticide composition is applied in an effective dose to the pest that needs to be controlled or to the medium in which it grows.

Images