Long-acting slow-release wormwood compound essential oil and preparation method thereof

Abstract

This invention discloses a long-acting, sustained-release Artemisia argyi compound essential oil and its preparation method, relating to the field of plant essential oil technology. The Artemisia argyi compound essential oil comprises the following raw material components: by weight, 92-106 parts of plant oil, 0.3-0.5 parts of vanillyl butyl ether, 0.05-0.15 parts of butylated hydroxytoluene, and 3-5 parts of a sustained-release agent; the plant oil is composed of grape seed oil, bitter orange blossom oil, and Artemisia argyi leaf oil; the plant oil is composed of grape seed oil, bitter orange blossom oil, and Artemisia argyi leaf oil in a mass ratio of (80-90):(7-9):(5-7). The Artemisia argyi compound essential oil prepared by this application has a long-acting, sustained-release function, effectively improving the transdermal absorption rate and antioxidant properties of the essential oil.

Description

Technical Field

[0001] This invention relates to the field of plant essential oil technology, specifically a long-acting, slow-release artemisia compound essential oil and its preparation method. Background Technology

[0002] As contemporary consumers' skincare concepts shift towards "natural, effective, and gentle," plant essential oils, with their naturally occurring antibacterial, anti-inflammatory, and antioxidant bioactivities, have become popular raw materials in cosmetics manufacturing due to their natural and additive-free nature, meeting current skincare demands. Among them, artemisia essential oil, with its soothing, anti-inflammatory, and gentle antibacterial properties, has become a popular ingredient in skincare.

[0003] In existing technologies, traditional skincare preparations of mugwort essential oil are still mainly based on pure oil or simple dilution. The base plant oil is often obtained through conventional physical pressing or high-temperature extraction processes. These methods have certain limitations: First, in the extraction of raw oil, conventional pressing and high-temperature extraction methods result in low oil yield and many impurities. These residual impurities become potential allergens, reducing the safety of use. Second, essential oils themselves are highly volatile. The small-molecule active ingredients in traditional preparations will quickly evaporate and be lost after contact with air, reducing the absorption of active ingredients. At the same time, the release of active ingredients in traditional essential oils is poor. Either the local concentration is too high, causing skin irritation such as stinging and redness, or the skin absorption is poor, resulting in insignificant efficacy.

[0004] Therefore, in order to solve the above problems, it is of great significance to provide a long-acting, slow-release Artemisia argyi compound essential oil and its preparation method. Summary of the Invention

[0005] The purpose of this invention is to provide a long-acting, sustained-release Artemisia argyi compound essential oil and its preparation method, so as to solve the problems raised in the prior art.

[0006] To achieve the above objectives, the present invention provides the following technical solution: A long-acting, sustained-release Artemisia argyi compound essential oil, wherein the Artemisia argyi compound essential oil comprises the following raw material components: by weight, 92-106 parts of vegetable oil, 0.3-0.5 parts of vanillyl butyl ether, 0.05-0.15 parts of butylated hydroxytoluene, and 3-5 parts of sustained-release agent; The vegetable oil is composed of grape seed oil, bitter orange blossom oil, and mugwort oil.

[0007] More optimally, the vegetable oil is composed of grape seed oil, bitter orange blossom oil and mugwort oil in a mass ratio of (80~90):(7~9):(5~7).

[0008] A more optimized method for extracting grape seed oil is as follows: Step 1: Dry and grind grape seeds, then add them to water, stir evenly, treat with ultrasound for 30-45 minutes, adjust the pH to 4.5-5.5, add cellulase, set the temperature to 45-55℃, and enzymatically hydrolyze for 2-3 hours; then adjust the pH to 6-7, add neutral protease, continue enzymatic hydrolysis for 3-4 hours, inactivate the enzyme at 90-100℃, and centrifuge at 4000-5000 rpm for 20-30 minutes; obtain the upper oil layer and the lower enzymatic hydrolysate. Step 2: The lower layer of enzymatic hydrolysate is subjected to a "freeze-thaw-centrifuge" cycle. The oils obtained from centrifugation during the cycle are combined and then combined with the upper layer of oils obtained in Step 1 to obtain grape seed oil.

[0009] In a more optimized manner, in step 1, the mass ratio of grape seed, cellulase, and neutral protease is (0.5~1.5):(0.01~0.02):(0.005~0.01).

[0010] In a more optimized manner, the freezing-thawing-centrifugation process is as follows: the lower layer of enzyme hydrolysate is frozen at -10~-20℃ for 2~4 hours, thawed naturally at room temperature, and centrifuged at 4000~5000rpm for 20~30 minutes; the cycle is repeated 5~10 times.

[0011] In a more optimized manner, the sustained-release agent is composed of modified hyaluronic acid and porous cyclodextrin in a mass ratio of (1.2~2):(1.8~3).

[0012] A more optimized method for preparing the modified hyaluronic acid is as follows: sodium hyaluronate is added to a glycerol aqueous solution and stirred until homogeneous. Then, 3-aminopropyltriethoxysilane and citric acid are added sequentially. The temperature is set at 30-35°C and the mixture is stirred for 8-12 hours. Ethanol is added for precipitation, followed by washing and drying to obtain the modified hyaluronic acid.

[0013] In a more optimized manner, the raw materials for the modified hyaluronic acid, by weight, are: 0.5-1.5 parts sodium hyaluronate, 0.1-0.5 parts 3-aminopropyltriethoxysilane, and 0.01-0.03 parts citric acid.

[0014] A more optimized method for preparing the porous cyclodextrin is as follows: Step 1: Add β-cyclodextrin and sodium chloride in a mass ratio of 1:(0.5~1.5) to water, spray dry, wash with water, and dry to obtain pore-forming cyclodextrin; Step 2: Add pore-forming cyclodextrin and sodium trimetaphosphate in a mass ratio of 1:(0.08~0.15) to water, stir evenly, adjust the pH to 10~11, set the temperature to 40~50℃, stir for 2~4 hours; adjust the pH to neutral, filter, wash, and dry to obtain porous cyclodextrin.

[0015] A method for preparing a long-acting, sustained-release Artemisia argyi compound essential oil includes the following steps: Step 1: Mix bitter orange blossom oil and mugwort oil in advance to obtain primary essential oil; Step 2: Add grape seed oil, butylated hydroxytoluene, and vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 40~50℃, and stir; add the slow-release agent and shear at high speed; cool down to 25~35℃ and stir to obtain the mugwort compound essential oil.

[0016] Compared with the prior art, the beneficial effects of the present invention are: 1. The mugwort compound essential oil prepared in this application uses grape seed oil as a mild base. Its rich vitamin E and unsaturated fatty acids moisturize and repair the skin barrier, while uniformly dispersing the active ingredients of bitter orange blossom oil and mugwort oil, reducing the irritation risk that may be caused by a single high-concentration essential oil. The linalool in bitter orange blossom oil and the eucalyptol in mugwort oil work synergistically to exert anti-inflammatory and soothing effects. The former can alleviate the mild irritation of mugwort oil and together enhance the anti-inflammatory and calming effect on the skin.

[0017] Grape seed oil is extracted using an enzyme-linked ultrasonic method. Ultrasonic pretreatment efficiently breaks down grape seed cell structures, and combined with the synergistic enzymatic hydrolysis of cellulase and neutral protease, fully dissolves the oil, removes impurities, and effectively improves extraction efficiency. Cellulase directionally decomposes cellulose and macromolecular impurities in the cell wall, disrupting the cell barrier and releasing the oil. Neutral protease degrades protein complexes in the oil, further enhancing its purity. Subsequent centrifugation and repeated freeze-thaw-centrifugation effectively separate and recover residual oil. Freeze-thaw precipitation ensures complete separation of incompletely decomposed protein residues from the oil, significantly reducing small-molecule sugars, peptides, and insoluble solids, minimizing impurity residue, and reducing the risk of skin allergies caused by impurities.

[0018] 2. A sustained-release agent is introduced into the essential oil formulation. This is achieved by forming a sustained-release system using modified hyaluronic acid and porous cyclodextrin. The modified hyaluronic acid undergoes amylation, and the amino groups effectively enhance the affinity and adsorption of the active ingredients in the essential oil, encapsulating the essential oil molecules within a linear network of hyaluronic acid. This delays the release of the active ingredients, thereby increasing skin absorption. The porous cyclodextrin, due to its porous structure, achieves interfacial affinity for the essential oil. Its abundant porous structure provides adsorption space for the active substances in the essential oil, optimizing the sustained-release properties of the active ingredients, improving transdermal absorption, and reducing essential oil sensitivity.

[0019] The synergistic effect of these two agents significantly slows down the volatilization of the active ingredients in the essential oils, improves the transdermal release process, and avoids the problem of excessively high local concentrations caused by uneven release in traditional essential oil products. Furthermore, the sustained-release agent promotes the uniform dispersion of the antioxidant butylated hydroxytoluene (BHT), thereby enhancing the antioxidant efficiency and stability of the essential oils. Detailed Implementation

[0020] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0021] In the following embodiments, the parts are by weight. It should be noted that there are no special restrictions on the purchase manufacturers of all the raw materials involved in this invention. Exemplary examples include: sodium hyaluronate (CAS: 9067-32-7), 3-aminopropyltriethoxysilane (CAS: 919-30-2), citric acid (CAS: 77-92-9), β-cyclodextrin (CAS: 7585-39-9), sodium chloride (CAS: 7647-14-5), sodium trimetaphosphate (CAS: 7785-84-4), cellulase (CAS: 9012-54-8), and neutral protease (CAS: 9068-59-1).

[0022] Pre-preparation: I. Preparation of modified hyaluronic acid: Add 1 part of sodium hyaluronate to 50 parts of glycerol aqueous solution (composed of 20 parts of glycerol and 30 parts of water), stir evenly, then add 0.3 parts of 3-aminopropyltriethoxysilane and 0.02 parts of citric acid in sequence, set the temperature to 35℃, stir for 10 hours, add ethanol for precipitation, wash, and dry to obtain modified hyaluronic acid.

[0023] II. Preparation of porous cyclodextrin: Step 1: Add β-cyclodextrin and sodium chloride in a mass ratio of 1:1 to water, spray dry, wash with water, and dry to obtain porous cyclodextrin; Step 2: Add pore-forming cyclodextrin and sodium trimetaphosphate in a mass ratio of 1:0.12 to water, stir evenly, adjust the pH to 10, set the temperature to 45℃, and stir for 2-4 hours; adjust the pH to neutral, filter, wash, and dry to obtain porous cyclodextrin.

[0024] III. Extraction of grape seed oil: Step 1: Dry and grind 1 part grape seeds, then add them to 100 parts water, stir well, sonicate for 40 minutes, adjust the pH to 5, add 0.015 parts cellulase, set the temperature to 50℃, and enzymatically hydrolyze for 2.5 hours; then adjust the pH to 7, add 0.007 parts neutral protease, and continue enzymatic hydrolysis for 3.5 hours, inactivate the enzyme at 90℃, and centrifuge at 4500 rpm for 25 minutes; obtain the upper oil and the lower enzymatic hydrolysate. Step 2: Perform a "freeze-thaw-centrifuge" cycle on the lower layer of enzymatic hydrolysate. Freeze the lower layer of enzymatic hydrolysate at -18℃ for 3 hours, thaw it naturally at room temperature, and centrifuge it at 4500rpm for 25 minutes. Repeat this cycle 7 times. Combine the oils obtained from centrifugation during the cycle and combine them with the upper layer of oils obtained in Step 1 to obtain grape seed oil.

[0025] Example 1: A method for preparing a long-acting, sustained-release Artemisia argyi compound essential oil, specifically including the following steps: I. Preparation of sustained-release agent: 1.6 parts of modified hyaluronic acid and 2.4 parts of porous cyclodextrin were added to 50 parts of deionized water in sequence, stirred evenly at 400 rpm, and soaked for 5 hours to obtain sustained-release agent.

[0026] II. Preparation of Artemisia argyi compound essential oil: Step 1: Mix 8 parts bitter orange blossom oil and 6 parts mugwort oil in advance to obtain the primary essential oil; Step 2: Add 0.1 parts of butylated hydroxytoluene, 85 parts of grape seed oil, and 0.4 parts of vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 45℃, and stir; add 4 parts of slow-release agent, shear at high speed, cool to 30℃, and stir for 25 minutes to obtain the Artemisia argyi compound essential oil.

[0027] Example 2: A method for preparing a long-acting, sustained-release Artemisia argyi compound essential oil, specifically including the following steps: I. Preparation of sustained-release agent: 1.2 parts of modified hyaluronic acid and 1.8 parts of porous cyclodextrin were added to 50 parts of deionized water in sequence, stirred evenly at 400 rpm, and soaked for 5 hours to obtain sustained-release agent.

[0028] II. Preparation of Artemisia argyi compound essential oil: Step 1: Mix 7 parts bitter orange blossom oil and 5 parts mugwort oil in advance to obtain the primary essential oil; Step 2: Add 0.05 parts butylated hydroxytoluene, 80 parts grape seed oil, and 0.3 parts vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 45℃, and stir; add 3 parts slow-release agent, shear at high speed, cool to 30℃, and stir for 25 minutes to obtain the Artemisia argyi compound essential oil.

[0029] Example 3: A method for preparing a long-acting, sustained-release Artemisia argyi compound essential oil, specifically including the following steps: 1. Preparation of sustained-release agent: Add 2 parts of modified hyaluronic acid and 3 parts of porous cyclodextrin to 50 parts of deionized water in sequence, stir evenly at 400 rpm, and soak for 5 hours to obtain sustained-release agent.

[0030] II. Preparation of Artemisia argyi compound essential oil: Step 1: Mix 8 parts bitter orange blossom oil and 6 parts mugwort oil in advance to obtain the primary essential oil; Step 2: Add 0.15 parts of butylated hydroxytoluene, 90 parts of grape seed oil, and 0.5 parts of vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 45℃, and stir; add 5 parts of slow-release agent, shear at high speed, cool to 30℃, and stir for 25 minutes to obtain the Artemisia argyi compound essential oil.

[0031] Comparative Example 1: Based on Example 1, the sustained-release agent components were adjusted, and modified hyaluronic acid was replaced with hyaluronic acid to prepare Artemisia argyi compound essential oil. The rest remained the same as in Example 1, specifically including the following steps: I. Preparation of sustained-release agent: 1.6 parts of hyaluronic acid and 2.4 parts of porous cyclodextrin were added to 50 parts of deionized water in sequence, stirred evenly at 400 rpm, and soaked for 5 hours to obtain sustained-release agent.

[0032] II. Preparation of Artemisia argyi compound essential oil: Step 1: Mix 8 parts bitter orange blossom oil and 6 parts mugwort oil in advance to obtain the primary essential oil; Step 2: Add 0.1 parts of butylated hydroxytoluene, 85 parts of grape seed oil, and 0.4 parts of vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 45℃, and stir; add 4 parts of slow-release agent, shear at high speed, cool to 30℃, and stir for 25 minutes to obtain the Artemisia argyi compound essential oil.

[0033] Comparative Example 2: Based on Example 1, the sustained-release agent components were adjusted, and porous cyclodextrin was replaced with cyclodextrin to prepare Artemisia argyi compound essential oil. The rest remained the same as in Example 1, specifically including the following steps: I. Preparation of sustained-release agent: 1.6 parts of modified hyaluronic acid and 2.4 parts of cyclodextrin were added to 50 parts of deionized water in sequence, stirred evenly at 400 rpm, and soaked for 5 hours to obtain sustained-release agent.

[0034] II. Preparation of Artemisia argyi compound essential oil: Step 1: Mix 8 parts bitter orange blossom oil and 6 parts mugwort oil in advance to obtain the primary essential oil; Step 2: Add 0.1 parts of butylated hydroxytoluene, 85 parts of grape seed oil, and 0.4 parts of vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 45℃, and stir; add 4 parts of slow-release agent, shear at high speed, cool to 30℃, and stir for 25 minutes to obtain the Artemisia argyi compound essential oil.

[0035] Comparative Example 3: Based on Example 1, the sustained-release agent components were adjusted, and porous cyclodextrin was replaced with modified hyaluronic acid to prepare Artemisia argyi compound essential oil. The rest remained the same as in Example 1, specifically including the following steps: Preparation of sustained-release agent: Add 4 parts of modified hyaluronic acid to 50 parts of deionized water, stir evenly at 400 rpm, and soak for 5 hours to obtain sustained-release agent.

[0036] II. Preparation of Artemisia argyi compound essential oil: Step 1: Mix 8 parts bitter orange blossom oil and 6 parts mugwort oil in advance to obtain the primary essential oil; Step 2: Add 0.1 parts of butylated hydroxytoluene, 85 parts of grape seed oil, and 0.4 parts of vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 45℃, and stir; add 4 parts of slow-release agent, shear at high speed, cool to 30℃, and stir for 25 minutes to obtain the Artemisia argyi compound essential oil.

[0037] Comparative Example 4: Based on Example 1, the sustained-release agent components were adjusted, and modified hyaluronic acid was replaced with porous cyclodextrin to prepare Artemisia argyi compound essential oil. The rest remained the same as in Example 1, specifically including the following steps: Preparation of sustained-release agent: Add 4 parts of porous cyclodextrin to 50 parts of deionized water, stir evenly at 400 rpm, and soak for 5 hours to obtain sustained-release agent.

[0038] Preparation of Artemisia argyi compound essential oil: Step 1: Mix 8 parts bitter orange blossom oil and 6 parts mugwort oil in advance to obtain the primary essential oil; Step 2: Add 0.1 parts of butylated hydroxytoluene, 85 parts of grape seed oil, and 0.4 parts of vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 45℃, and stir; add 4 parts of slow-release agent, shear at high speed, cool to 30℃, and stir for 25 minutes to obtain the Artemisia argyi compound essential oil.

[0039] Comparative Example 5: Based on Example 1, without adding a slow-release agent, a compound essential oil of Artemisia argyi was prepared, and the rest remained the same as in Example 1, specifically including the following steps: Step 1: Mix 8 parts bitter orange blossom oil and 6 parts mugwort oil in advance to obtain the primary essential oil; Step 2: Add 0.1 parts of butylated hydroxytoluene, 85 parts of grape seed oil, and 0.4 parts of vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 45℃, and stir; cool down to 30℃ and stir for 25 minutes to obtain the mugwort compound essential oil.

[0040] Comparative Example 6: Based on Example 1, the grape seed oil extraction process was adjusted, and the neutral protease was replaced with cellulase to prepare Artemisia argyi compound essential oil; the rest remained the same as in Example 1, specifically including the following steps: I. Extraction of grape seed oil: Step 1: Dry and grind 1 part of grape seeds, then add them to 100 parts of water, stir well, sonicate for 40 minutes, adjust the pH to 5, add 0.015 parts of cellulase, set the temperature to 50℃, and enzymatically hydrolyze for 2.5 hours; inactivate the enzyme at 90℃, centrifuge at 4500rpm for 25 minutes; obtain the upper oil and the lower enzymatic hydrolysate. Step 2: Perform a "freeze-thaw-centrifuge" cycle on the lower layer of enzymatic hydrolysate. Freeze the lower layer of enzymatic hydrolysate at -18℃ for 3 hours, thaw it naturally at room temperature, and centrifuge it at 4500rpm for 25 minutes. Repeat this cycle 7 times. Combine the oils obtained from centrifugation during the cycle and combine them with the upper layer of oils obtained in Step 1 to obtain grape seed oil.

[0041] II. Preparation of sustained-release agent: 1.6 parts of modified hyaluronic acid and 2.4 parts of porous cyclodextrin were added to 50 parts of deionized water in sequence, stirred evenly at 400 rpm, and soaked for 5 hours to obtain sustained-release agent.

[0042] III. Preparation of Artemisia argyi compound essential oil: Step 1: Mix 8 parts bitter orange blossom oil and 6 parts mugwort oil in advance to obtain the primary essential oil; Step 2: Add 0.1 parts of butylated hydroxytoluene, 85 parts of grape seed oil, and 0.4 parts of vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 45℃, and stir; add 4 parts of slow-release agent, shear at high speed, cool to 30℃, and stir for 25 minutes to obtain the Artemisia argyi compound essential oil.

[0043] Performance testing: (1) Transdermal absorption rate: 1 mL of the Artemisia argyi compound essential oil prepared in Examples 1-3 and Comparative Examples 1-6 was applied to the skin outside the body. The temperature was set at 37℃. After 6 hours of action, all the receiving liquid was taken out as the sample liquid and filtered with a 0.22μm microporous membrane. The concentration and maximum release of the Artemisia argyi compound essential oil were measured, and the absorption rate was calculated. Absorption rate = total absorption / maximum release × 100%. The transdermal absorption rate can reflect the degree of slow release of the essential oil from the side. At the same time, it can also help to judge whether the essential oil components are more volatilized and lost in the air or effectively absorbed and utilized through the skin.

[0044] (2) Irritation test: The Artemisia argyi compound essential oils of Examples 1-3 and Comparative Examples 1-6 were subjected to skin irritation test. 24 hours before the test, the hair on both sides of the spine of Chinese white rabbits was cut off. The hair removal area was 3cm×3cm on each side. The oil was applied to the hairless skin on one side with an area of ​​2.5cm×2.5cm. The hairless skin on the other side was used as a blank control. After 4 hours after application, the oil was washed off with water. The oil was applied once a day for 14 consecutive days. The skin condition was observed 24 hours after each application.

[0045] (3) DPPH free radical scavenging rate: The antioxidant properties of the sample were tested by using the DPPH free radical scavenging rate: an appropriate amount of the Artemisia argyi compound essential oil to be tested was prepared into a sample solution with anhydrous ethanol and reacted with DPPH ethanol solution in the dark for 30 min. The absorbance was measured at a wavelength of 510 nm and the free radical scavenging rate was calculated.

[0046]

[0047] Conclusion: The above data shows that the Artemisia argyi compound essential oil exhibits excellent comprehensive performance in terms of sustained-release properties, safety, and antioxidant activity. On one hand, the sustained-release system formed by the combination of modified hyaluronic acid and porous cyclodextrin enhances transdermal absorption, thereby improving the utilization efficiency of the essential oil. Furthermore, the enzyme-linked ultrasound and repeated freeze-thaw-centrifugation process used in grape seed oil extraction efficiently breaks down cell walls and fully releases the oil under gentle conditions, while effectively removing sensitizing impurities and reducing the risk of allergies. The synergistic effect of the sustained-release agent and the refined extraction process jointly ensures the high absorption rate, low irritation, and stable antioxidant effect of the essential oil.

[0048] Of the comparative examples 1-6, the irritation increased in examples 1, 5, and 6: Example 1 had weak adsorption capacity due to unmodified hyaluronic acid; Example 5, lacking any sustained-release agent, experienced rapid evaporation and direct skin contact, leading to decreased transdermal absorption and increased irritation; Example 6, lacking neutral protease, resulted in residual impurities in the grape seed base oil, increasing skin irritation. Examples 2, 3, and 4 showed decreased transdermal absorption due to the lack of a sustained-release system. Example 2 lacked a porous structure; Examples 3 and 4 used single components, failing to achieve synergistic sustained release, resulting in excessively rapid release of essential oil active ingredients and reduced transdermal absorption. In conclusion, using modified hyaluronic acid and porous cyclodextrin as sustained-release agents effectively inhibits the volatilization of essential oil active ingredients in essential oil systems, enhancing the long-lasting sustained-release effect of essential oils.

[0049] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A long-acting, sustained-release mugwort compound essential oil, characterized in that: The Artemisia argyi compound essential oil comprises the following raw material components: by weight, 92-106 parts vegetable oil, 0.3-0.5 parts vanillyl butyl ether, 0.05-0.15 parts butylated hydroxytoluene, and 3-5 parts sustained-release agent; The vegetable oil is composed of grape seed oil, bitter orange blossom oil, and mugwort oil.

2. The long-acting, sustained-release mugwort compound essential oil according to claim 1, characterized in that: The vegetable oil is composed of grape seed oil, bitter orange blossom oil and mugwort oil in a mass ratio of (80~90):(7~9):(5~7).

3. The long-acting, sustained-release mugwort compound essential oil according to claim 1, characterized in that: The method for extracting grape seed oil is as follows: Step 1: Dry and grind grape seeds, then add them to water, stir evenly, treat with ultrasound for 30-45 minutes, adjust the pH to 4.5-5.5, add cellulase, set the temperature to 45-55℃, and enzymatically hydrolyze for 2-3 hours; then adjust the pH to 6-7, add neutral protease, continue enzymatic hydrolysis for 3-4 hours, inactivate the enzyme at 90-100℃, and centrifuge at 4000-5000 rpm for 20-30 minutes; obtain the upper oil layer and the lower enzymatic hydrolysate. Step 2: The lower layer of enzymatic hydrolysate is subjected to a "freeze-thaw-centrifuge" cycle. The oils obtained by centrifugation during the cycle are combined and then combined with the upper layer of oils obtained in Step 1 to obtain grape seed oil.

4. The long-acting, sustained-release mugwort compound essential oil according to claim 3, characterized in that: In step 1, the mass ratio of grape seed, cellulase, and neutral protease is (0.5~1.5):(0.01~0.02):(0.005~0.01).

5. The long-acting, sustained-release mugwort compound essential oil according to claim 3, characterized in that: The freezing-thawing-centrifugation process is as follows: the lower layer of enzyme hydrolysate is frozen at -10~-20℃ for 2~4 hours, thawed naturally at room temperature, and centrifuged at 4000~5000rpm for 20~30 minutes; the cycle is repeated 5~10 times.

6. The long-acting, sustained-release mugwort compound essential oil according to claim 1, characterized in that: The sustained-release agent is composed of modified hyaluronic acid and porous cyclodextrin in a mass ratio of (1.2~2):(1.8~3).

7. The long-acting, sustained-release mugwort compound essential oil according to claim 6, characterized in that: The modified hyaluronic acid is prepared by adding sodium hyaluronate to a glycerol aqueous solution, stirring until homogeneous, then adding 3-aminopropyltriethoxysilane and citric acid sequentially, setting the temperature to 30-35℃, stirring for 8-12 hours, adding ethanol for precipitation, washing, and drying to obtain modified hyaluronic acid.

8. The long-acting, sustained-release mugwort compound essential oil according to claim 7, characterized in that: The modified hyaluronic acid raw materials, by weight, consist of: 0.5-1.5 parts sodium hyaluronate, 0.1-0.5 parts 3-aminopropyltriethoxysilane, and 0.01-0.03 parts citric acid.

9. The long-acting, sustained-release mugwort compound essential oil according to claim 6, characterized in that: The method for preparing the porous cyclodextrin is as follows: Step 1: Add β-cyclodextrin and sodium chloride in a mass ratio of 1:(0.5~1.5) to water in sequence, spray dry, wash with water, and dry to obtain pore-forming cyclodextrin; Step 2: Add pore-forming cyclodextrin and sodium trimetaphosphate in a mass ratio of 1:(0.08~0.15) to water in sequence, stir evenly, adjust the pH to 10~11, set the temperature to 40~50℃, stir for 2~4 hours; adjust the pH to neutral, filter, wash, and dry to obtain porous cyclodextrin.

10. The method for preparing a long-acting, sustained-release Artemisia argyi compound essential oil according to claim 1, characterized in that: Includes the following steps: Step 1: Mix bitter orange blossom oil and mugwort oil in advance to obtain primary essential oil; Step 2: Add grape seed oil, butylated hydroxytoluene, and vanillyl butyl ether to the primary essential oil in sequence, set the temperature to 40~50℃, and stir; add the slow-release agent and shear at high speed; cool down to 25~35℃ and stir to obtain the mugwort compound essential oil.

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