Production and preparation method of anti-alopecia apple concentrated powder and application thereof

Abstract

This invention relates to the field of natural product extraction technology, and provides a method for producing and applying concentrated apple powder for preventing hair loss and promoting hair growth. The method includes the following steps: S1, selecting fresh apples, washing, cutting, drying, crushing, sieving, and storing in a sealed container at 3-5℃ to obtain apple powder for later use; S2, taking the apple powder from step S1, adding an extraction solvent, setting the temperature, and performing ultrasonic-microwave combined extraction, repeating the extraction once, filtering, centrifuging, taking the supernatant, and concentrating it under vacuum rotary evaporation at 45-55℃ to obtain a concentrated solution for later use; S3, pretreating macroporous resin, then adsorbing the concentrated solution from step S2 onto the pretreated macroporous resin, washing with water, washing with alcohol, vacuum concentrating, filtering, and freeze-drying to obtain concentrated apple powder. This invention provides concentrated apple powder for preventing hair loss and promoting hair growth, involving a green and environmentally friendly extraction method with a high extraction rate of apple polyphenols, which can be applied to the fields of hair growth and hair loss prevention.

Description

Technical Field

[0001] This invention relates to the field of natural product extraction technology, and in particular to a method for producing and applying concentrated apple powder for preventing hair loss and promoting hair growth. Background Technology

[0002] With increasing work and life pressures, as well as the deterioration of the external environment, hair loss has become a global health problem. According to statistics, about 1 billion people worldwide suffer from hair loss. Traditional treatments mainly include drug treatment, hair transplantation, or cosmetic care, but these methods may have side effects, high costs, or insignificant effects.

[0003] Apples are rich in apple polyphenols, which are a general term for phenolic compounds found in apples, such as proanthocyanidins B2, proanthocyanidins B1, and catechins. They have a strong antioxidant effect, which can protect the scalp and hair from the harmful effects of oxidative stress and reactive oxygen species, and have a positive effect on hair growth.

[0004] Currently, the most common extraction method for apple polyphenols is organic solvent extraction. Methanol, ethanol, and acetone are commonly used organic solvents for extracting phenolic compounds. However, these traditional organic solvents are usually highly toxic, flammable, or volatile, which can easily cause environmental pollution. Using green solvents to replace toxic organic solvents to extract valuable compounds from biomass has become a research hotspot in the fields of analytical chemistry and biochemistry. In addition, the impurity content of extracts currently on the market is relatively high, and there are problems with imprecise purification processes, which seriously affect the safety and efficacy of the products.

[0005] Patent CN 106866606 A discloses a method for efficiently extracting proanthocyanidins from young apples, comprising the following steps: 1) whole-body crushing and pulping; 2) alcohol solution extraction; 3) centrifugation; 4) inorganic membrane impurity removal; 5) nanofiltration membrane concentration; 6) resin adsorption; and 7) spray drying. This method yields high-purity proanthocyanidins, enabling the transformation of young apples from waste into valuable resources and high-value utilization. Although it involves inorganic membrane impurity removal and resin adsorption processes, the process still uses the traditional ethanol extraction medium, which still poses the problem of environmental pollution.

[0006] Therefore, there is an urgent need in the market for an apple concentrate powder that involves a green and environmentally friendly extraction method with a high extraction rate of apple polyphenols, which can be applied to the fields of hair growth and hair loss prevention. Summary of the Invention

[0007] To address the problems existing in the prior art, this invention discloses a method for preparing apple concentrate powder. It designs a green and environmentally friendly extraction solvent, combines specific temperature and ultrasonic-microwave extraction, and uses a specific type of macroporous resin for purification. This method has the advantage of high extraction rate of apple polyphenols and has good effects on hair growth and hair loss prevention.

[0008] To achieve the above objectives, the technical solution adopted by the present invention is as follows: This invention provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth, comprising the following steps: S1. Select fresh apples, wash them with tap water and then with deionized water. Cut the apples into pieces, dry them, crush them, sieve them, and store them in a sealed container at 3-5℃ to obtain apple powder for later use. S2. Take the apple powder from step S1, add the extraction solvent, set the temperature, and perform ultrasonic-microwave combined extraction. Repeat the extraction once, filter, centrifuge, take the supernatant, and concentrate it by vacuum rotary evaporation at 45-55℃ to obtain the concentrated solution for later use. S3. Pre-treat the macroporous resin, then adsorb the concentrate from step S2 onto the pre-treated macroporous resin, wash with water, wash with alcohol, vacuum concentrate, filter, and freeze dry to obtain apple concentrate powder for preventing hair loss and promoting hair growth.

[0009] In some embodiments of the present invention, in step S2, the ratio of apple powder to extraction solvent is 1g:(24-26)ml.

[0010] Preferably, in step S2, the ratio of apple powder to extraction solvent is 1g:25ml.

[0011] In some embodiments of the present invention, step S2, the method for preparing the extraction solvent includes the following steps: Mix choline chloride and lactic acid, heat to 75-85℃, stir for 3-5 hours, cool, add deionized water, and stir to obtain the extraction solvent.

[0012] In some embodiments of the present invention, the molar ratio of choline chloride to lactic acid is 1:(1-1.8).

[0013] Preferably, the molar ratio of choline chloride to lactic acid is 1:1.5.

[0014] In some embodiments of the present invention, the water content of the extraction solvent is 20-30 wt%.

[0015] Preferably, the water content of the extraction solvent is 25%.

[0016] In some embodiments of the present invention, in step S2, the temperature is set to 40-50°C and the extraction time is 35-45 min.

[0017] In some embodiments of the present invention, in step S2, the ultrasonic power in the ultrasonic-microwave combined extraction is 90-110W, and the microwave power is 280-320W.

[0018] The applicant uses choline chloride and lactic acid as raw materials to prepare a eutectic solvent, which is then reacted with deionized water to obtain a green and environmentally friendly extraction solvent. On one hand, by controlling the molar ratio of choline chloride and lactic acid, the applicant can form stable hydrogen bonds with phenolic substances, resulting in highly efficient extraction capabilities. On the other hand, lactic acid acts as a hydrogen bond donor, providing free hydrogen atoms. + This promotes the diffusion of phenolic substances into the extraction solvent, and the polarity of the organic acid group of lactic acid is close to that of phenolic substances, which can dissolve more apple polyphenols. On the other hand, the applicant's addition of an appropriate amount of deionized water can reduce the viscosity of the extraction solvent without destroying the hydrogen bond structure between choline chloride and lactic acid to ensure good interaction with phenolic substances, thereby improving mass transfer efficiency and shortening extraction time, which is beneficial to improving the extraction rate of apple polyphenols.

[0019] Furthermore, the applicant controls the ratio of apple powder to extraction solvent to ensure sufficient contact area between the extraction solvent and the apple, resulting in efficient extraction while simultaneously avoiding the problem of reduced apple polyphenol yield due to the dissolution of other impurities or oxidation of phenolic substances.

[0020] Furthermore, the applicant set a suitable temperature and combined it with ultrasound and microwave extraction. While avoiding the decomposition of apple polyphenols, the mechanical vibration and cavitation effect of ultrasound and the high-frequency vibration of microwaves synergistically improved the extraction rate of apple polyphenols by the extraction solvent.

[0021] In some embodiments of the present invention, in step S3, the macroporous resin is of type NKA-9.

[0022] In some embodiments of the present invention, the method for preparing the pretreated macroporous resin in step S3 is as follows: The macroporous resin was soaked in anhydrous ethanol, washed with deionized water until no alcohol odor remained, soaked in sodium hydroxide aqueous solution, washed with deionized water until neutral, and soaked in hydrochloric acid aqueous solution, washed with deionized water until neutral to obtain pretreated macroporous resin.

[0023] In some embodiments of the present invention, the mass ratio of the apple powder to the pretreated macroporous resin is 1:(1.2-2).

[0024] Preferably, the mass ratio of the apple powder to the pretreated macroporous resin is 1:1.5.

[0025] The applicant selected a specific type of macroporous resin, NKA-9, for purification and adsorption treatment of the concentrate. The surface polarity and pore size distribution of NKA-9 are also compatible with apple polyphenols, giving it the advantage of high adsorption selectivity. Furthermore, the applicant pretreated the macroporous resin NKA-9 to remove impurities and open its pore structure, thereby improving the adsorption performance of the macroporous resin NKA-9. By controlling its dosage, the applicant achieved cost savings while ensuring sufficient adsorption of polyphenols, reducing the loss of apple polyphenols, and increasing the extraction rate of apple polyphenols.

[0026] In some embodiments of the present invention, the apple concentrate is apple polyphenol.

[0027] In another aspect, the present invention provides the application of the apple concentrate powder obtained by the aforementioned production method in the fields of hair growth and hair loss prevention.

[0028] Compared with the prior art, the present invention has the following beneficial effects: (1) This invention discloses a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. It designs a green and environmentally friendly extraction solvent, combines specific temperature and ultrasonic-microwave extraction, and uses a specific type of macroporous resin for purification. It has the advantage of high extraction rate of apple polyphenols and can be applied to the fields of hair growth and preventing hair loss, with good results.

[0029] (2) The present invention uses choline chloride and lactic acid as raw materials to prepare a eutectic solvent, and then uses deionized water to prepare a green and environmentally friendly extraction solvent. By controlling the molar ratio between choline chloride and lactic acid and adding an appropriate amount of deionized water, the ratio of apple powder to extraction solvent is further limited. With the addition of appropriate temperature and ultrasonic and microwave frequencies, the extraction rate of apple polyphenols is synergistically improved.

[0030] (3) In this invention, a certain amount of a specific type of macroporous resin NKA-9 is selected to purify and adsorb the concentrate. This saves costs while ensuring sufficient adsorption of apple polyphenols, reducing the loss of apple polyphenols and improving the extraction rate of apple polyphenols. Detailed Implementation

[0031] The present invention will be described below with reference to specific embodiments. It should be noted that the following embodiments are examples of the present invention and are used only to illustrate the invention, not to limit it. Other combinations and various modifications within the scope of the present invention can be made without departing from its spirit or scope.

[0032] In the following examples and comparative examples, except for the extraction solvent, all other compound monomers and related reagents used were commercially available. The apples used were commercially available Fuji apples, and the macroporous resin was NKA-9.

[0033] Preparation Example 1 The preparation method of pretreated macroporous resin NKA-9 includes the following steps: The macroporous resin NKA-9 was soaked in anhydrous ethanol for 24 hours, washed with deionized water until no alcohol odor was detected, soaked in 5wt% sodium hydroxide aqueous solution for 12 hours, washed with deionized water until neutral, and soaked in 5wt% hydrochloric acid aqueous solution for 12 hours, washed with deionized water until neutral, thus obtaining the pretreated macroporous resin NKA-9.

[0034] Preparation Example 2 The method for synthesizing solvent A includes the following steps: Mix 1 mol of choline chloride and 1.5 mol of lactic acid, heat to 80°C, stir until a homogeneous, transparent, and stable liquid system is formed, cool to room temperature, add 90 ml of deionized water, and stir until homogeneous to obtain extraction solvent A.

[0035] Preparation Example 3 Extraction solvent B is implemented in the same way as extraction solvent A, except that the molar amount of lactic acid is replaced with 0.8 mol.

[0036] Preparation Example 4 Extraction solvent C is implemented in the same way as extraction solvent A, except that the number of moles of lactic acid is replaced with 2 mol.

[0037] Preparation Example 5 Extraction solvent D is implemented in the same way as extraction solvent A, except that deionized water is not added.

[0038] Preparation Example 6 Extraction solvent E is implemented in the same way as extraction solvent A, except that the volume of deionized water is replaced with 150 ml.

[0039] Example 1 A method for producing apple concentrate powder for preventing hair loss and promoting hair growth includes the following steps: S1. Select fresh apples, wash them with tap water and then wash them with deionized water. Cut the apples into pieces (10mm×10mm), dry them at 50℃ to constant weight, crush them, pass them through a 60-mesh sieve, and store them in a sealed container at 4℃ to obtain apple powder for later use. S2. Take 10g of apple powder from step S1, add 250ml of extraction solvent A, set the temperature to 45℃, and perform ultrasonic-microwave combined extraction for 40min (ultrasonic power 100W, microwave power 300W). Repeat the extraction once, filter (0.45μm filter membrane), centrifuge, take the supernatant, and concentrate it at 50℃ under vacuum rotary evaporation until the solid content is 30% to obtain the concentrated solution for later use. S3. The concentrate from step S2 is adsorbed onto 15g of pretreated macroporous resin NKA-9, washed with deionized water (3BV, flow rate 2BV / h), washed with 70wt% ethanol aqueous solution (4BV, flow rate 1BV / h), concentrated under vacuum at 35℃ to a solid content of 40%, filtered (0.45μm filter membrane), and freeze-dried for 36h (-35℃, vacuum degree 200Pa) to obtain apple concentrate powder for preventing hair loss and promoting hair growth.

[0040] Example 2 A method for producing apple concentrate powder for preventing hair loss and promoting hair growth includes the following steps: S1. Select fresh apples, wash them with tap water and then wash them with deionized water. Cut the apples into pieces (10mm×10mm), dry them at 50℃ to constant weight, crush them, pass them through a 60-mesh sieve, and store them in a sealed container at 3℃ to obtain apple powder for later use. S2. Take 10g of apple powder from step S1, add 240ml of extraction solvent A, set the temperature to 40℃, and perform ultrasonic-microwave combined extraction for 45min (ultrasonic power 90W, microwave power 280W). Repeat the extraction once, filter (0.45μm filter membrane), centrifuge, take the supernatant, and concentrate it at 45℃ by vacuum rotary evaporation until the solid content is 30% to obtain the concentrated solution for later use. S3. The concentrate from step S2 is adsorbed onto 12g of pretreated macroporous resin NKA-9, washed with deionized water (3BV, flow rate 2BV / h), washed with 70wt% ethanol aqueous solution (4BV, flow rate 1BV / h), concentrated under vacuum at 35℃ to a solid content of 40%, filtered (0.45μm filter membrane), and freeze-dried for 36h (-35℃, vacuum degree 200Pa) to obtain apple concentrate powder for preventing hair loss and promoting hair growth.

[0041] Example 3 A method for producing apple concentrate powder for preventing hair loss and promoting hair growth includes the following steps: S1. Select fresh apples, wash them with tap water and then wash them with deionized water. Cut the apples into pieces (10mm×10mm), dry them at 50℃ to constant weight, crush them, pass them through a 60-mesh sieve, and store them in a sealed container at 5℃ to obtain apple powder for later use. S2. Take 10g of apple powder from step S1, add 260ml of extraction solvent A, set the temperature to 50℃, and perform ultrasonic-microwave combined extraction for 35min (ultrasonic power 110W, microwave power 320W). Repeat the extraction once, filter (0.45μm filter membrane), centrifuge, take the supernatant, and concentrate it at 55℃ by vacuum rotary evaporation until the solid content is 30% to obtain the concentrated solution for later use. S3. The concentrate from step S2 is adsorbed onto 20g of pretreated macroporous resin NKA-9, washed with deionized water (3BV, flow rate 2BV / h), washed with 70wt% ethanol aqueous solution (4BV, flow rate 1BV / h), concentrated under vacuum at 35℃ to a solid content of 40%, filtered (0.45μm filter membrane), and freeze-dried for 36h (-35℃, vacuum degree 200Pa) to obtain apple concentrate powder for preventing hair loss and promoting hair growth.

[0042] Example 4 This embodiment provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. The specific implementation method is the same as in Embodiment 1, except that the volume of solvent A extracted in step S2 is replaced with 200ml.

[0043] Example 5 This embodiment provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. The specific implementation method is the same as in Embodiment 1, except that the volume of solvent A extracted in step S2 is replaced with 300ml.

[0044] Example 6 This embodiment provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. The specific implementation method is the same as in Embodiment 1, except that in step S2, the ultrasonic power is replaced with 50W and the microwave power is replaced with 200W in the ultrasonic-microwave combined extraction.

[0045] Example 7 This embodiment provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. The specific implementation method is the same as in Embodiment 1, except that extraction solvent B is used to replace extraction solvent A in an equal amount.

[0046] Example 8 This embodiment provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. The specific implementation method is the same as in Embodiment 1, except that extraction solvent C is used to replace extraction solvent A in equal amounts.

[0047] Example 9 This embodiment provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. The specific implementation method is the same as in Embodiment 1, except that extraction solvent D is used to replace extraction solvent A in equal amounts.

[0048] Example 10 This embodiment provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. The specific implementation method is the same as in Embodiment 1, except that extraction solvent A is replaced by an equal amount of extraction solvent E.

[0049] Example 11 This embodiment provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. The specific implementation method is the same as in Embodiment 1, except that an extraction solvent A is replaced by an equal amount of methanol aqueous solution with a volume fraction of 80%.

[0050] Example 12 This embodiment provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. The specific implementation method is the same as in embodiment 1, except that the macroporous resin type is replaced with AB-8 in step S3.

[0051] Example 13 This embodiment provides a method for producing concentrated apple powder for preventing hair loss and promoting hair growth. The specific implementation method is the same as in embodiment 1, except that the mass of macroporous resin in step S3 is replaced with 8g.

[0052] Performance testing The extraction rate of apple polyphenols from the apple concentrate powders of Examples 1-13 above was tested, and the test results are shown in Table 1.

[0053] 1) Determination principle: Polyphenolic compounds have active hydroxyl groups, which are easily oxidized by losing hydrogen under alkaline conditions. It can react with Folin-Ciocalteu reagent to form a blue substance, which has maximum absorption at 765 nm, under certain conditions. Following the Lambert-Beer Law, gallic acid (a reference material) can be used to determine the equivalent content of polyphenols in apples (referencing the relevant determination principles and methods in GB / T8313-2008). Based on this principle, a standard curve for the reference material is plotted, and the regression equation is derived. Then, a processed apple polyphenol sample is taken, its absorbance is measured, and the apple polyphenol content in the sample solution is calculated based on the regression equation.

[0054] 2) Determination method: Folin-Ciocalteu method A. Establishment of the standard curve: Take 0.5 ml of standard gallic acid solutions of different concentrations (0, 5, 10, 20, 30, 40, 50 μg / ml), add 1.0 ml of Folin C reagent, and after 5 min, add 2 ml of 10 wt% Na2CO3 solution. Incubate in a 50℃ water bath for 5 min, cool, and then measure the absorbance of the solution at 765 nm using a spectrophotometer. 765 Then, the apple polyphenol content C was obtained based on the standard curve. 多酚 Its standard curve is: C 多酚 =x A 765 —y,R 2 =Z (The coefficient of Z needs to be greater than 0.999 here) B. Sample solution content determination: Weigh 0.2g of apple concentrate powder, add 80ml of 50wt% ethanol aqueous solution, sonicate to prepare the solution, and then make up to 100ml to obtain apple concentrate powder extract. Take 0.5ml and add 1.0ml of Folin C reagent. After 5min, add 2ml of Na2CO3 solution, keep in a 50℃ water bath for 5min, cool, and then use a spectrophotometer to measure the absorbance of the solution at 765nm. 765 Substituting the values ​​into the standard curve, the apple polyphenol content C was calculated. 多酚 .

[0055] 3) Preparation of main reagents: gallic acid, Folin-Ciocalteu reagent, 10% Na2CO3 solution.

[0056] 4) Calculation method for apple polyphenol extraction rate: Apple polyphenol extraction rate (%) = [(C × V × N) / m1] / [(C0 × V0) / m0] × 100%.

[0057] C: Polyphenol concentration in apple concentrate extract (μg / ml) V: Total volume of apple concentrate extract (ml) = 100ml N: Dilution factor of apple concentrate extract m1: Apple concentrate mass (g) = 0.2g C0: Polyphenol concentration in apple powder extract (μg / ml) (determined using the same method) V0: Total volume of apple powder extract (ml) m0: Mass of apple powder (g) = 10g Table 1 As shown in Table 1, the apple polyphenol extraction rates of the apple concentrate powder in Examples 1-3 of this invention are all relatively high, and higher than the extraction rate obtained by using methanol-water solution in Example 11. Specifically, Examples 4-5 involved changes in the amount of extraction solvent; Example 6 involved changes in the ultrasonic and microwave conditions during the ultrasonic-microwave combined extraction, leading to varying degrees of decreased extraction efficiency and consequently a decrease in the apple polyphenol extraction rate; Examples 7-10 involved changes in the ratio of the main substances during the preparation of the extraction solvent, resulting in a decrease in the solvent's ability to react with phenolic substances, thus significantly reducing the apple polyphenol extraction rate; Examples 12-13 involved changes in the type and amount of macroporous resin, leading to a decrease in purification and adsorption efficiency, and consequently, a decrease in the apple polyphenol extraction rate.

[0058] The hair growth effect of the apple concentrate powder in Example 1 was tested, and the test results are shown in Table 2.

[0059] (1) Experimental materials: Mouse whisker hair follicles; Control group: Culture medium: Williams' E serum-free medium (Gibco, Lot: 2066552), containing 2 mM L Glutamine, 10 μg / mL bovine insulin, 10 μg / mL hydrocortisone, 100 U / mL penicillin, 100 μg / mL streptomycin; Positive group: Minoxidil liniment was prepared to a concentration of 10 μM using culture medium. (Minoxidil liniment, Sichuan Meida Kanghua Pharmaceutical Co., Ltd.) Sample group: The apple concentrate from Example 1 was prepared to a concentration of 70 mg / mL using culture medium.

[0060] (2) Experimental steps 1) Hair follicle separation: C57BL / 6 mice were humanely sacrificed, and the whiskers on both sides were cut off. The upper lip was disinfected three times with 75wt% ethanol. The whisker pads were cut off and placed in a 1:1 (volume) HBSS:PBS (PBS contains 100U / mL penicillin and 100μg / mL streptomycin). The follicles were rinsed three times with a 1:1 (volume) HBSS:PBS. The connective tissue around the hair follicles was carefully removed under a dissecting microscope. The hair follicles were bluntly separated from the surrounding tissue in the direction of the hair follicles with microsurgical forceps. The distal end of the hair follicle was gently grasped and the hair was carefully pulled back from the subcutaneous tissue. The separated hair follicles were placed in different wells of a 24-well plate. 2) Hair follicle culture: Incubate at 37℃ and 5% CO2 for 14 days, with 0.5 mL of culture medium per well; 3) Sample addition: Add samples the day after incubation, adding culture medium containing different concentrations of samples to the corresponding wells. The group was set up with 8 replicates, with 2-3 detached hair follicles placed in each well, and the solution was changed every 3 days. 4) Image analysis: The length of the whisker hair follicles was photographed on day 1 and day 14, and the morphological observation and measurement of the whisker hair follicle length were performed.

[0061] (3) Data Analysis The data is expressed as a mean. The relative growth rate of hair follicles is calculated using the following formula: Relative growth rate of hair follicles (%) = (Average hair follicle length of each group (D14)) Average hair follicle length (D0) of each group / (hair follicle length of the control group) Length (D14) Control group hair follicle length (D0) × 100% Table 2 As shown in Table 2, the apple concentrate in Example 1 of this invention has a good effect on hair growth.

[0062] The above embodiments are only for illustrating the technical concept and features of the present invention, and are intended to enable those skilled in the art to understand the content of the present invention and implement it. They should not be used to limit the scope of protection of the present invention. All equivalent changes or modifications made in accordance with the spirit and essence of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A method for producing concentrated apple powder for preventing hair loss and promoting hair growth, characterized in that, Includes the following steps: S1. Select fresh apples, wash them with tap water and then with deionized water. Cut the apples into pieces, dry them, crush them, sieve them, and store them in a sealed container at 3-5℃ to obtain apple powder for later use. S2. Take the apple powder from step S1, add the extraction solvent, set the temperature, and perform ultrasonic-microwave combined extraction. Repeat the extraction once, filter, centrifuge, take the supernatant, and concentrate it by vacuum rotary evaporation at 45-55℃ to obtain the concentrated solution for later use. S3. Pre-treat the macroporous resin, then adsorb the concentrate from step S2 onto the pre-treated macroporous resin, wash with water, wash with alcohol, vacuum concentrate, filter, and freeze dry to obtain apple concentrate powder for preventing hair loss and promoting hair growth.

2. The method for producing apple concentrate powder for preventing hair loss and promoting hair growth according to claim 1, characterized in that, In step S2, the ratio of apple powder to extraction solvent is 1g:(24-26)ml.

3. The method for producing apple concentrate powder for preventing hair loss and promoting hair growth according to claim 1, characterized in that, In step S2, the method for preparing the extraction solvent includes the following steps: Mix choline chloride and lactic acid, heat to 75-85℃, stir for 3-5 hours, cool, add deionized water, and stir to obtain the extraction solvent.

4. The method for producing apple concentrate powder for preventing hair loss and promoting hair growth according to claim 3, characterized in that, The molar ratio of choline chloride to lactic acid is 1:(1-1.8).

5. The method for producing apple concentrate powder for preventing hair loss and promoting hair growth according to claim 3, characterized in that, The water content of the extraction solvent is 20-30 wt%.

6. The method for producing apple concentrate powder for preventing hair loss and promoting hair growth according to claim 1, characterized in that, In step S2, the temperature is set to 40-50℃ and the extraction time is 35-45 min.

7. The method for producing apple concentrate powder for preventing hair loss and promoting hair growth according to claim 1, characterized in that, In step S2, the ultrasonic power in the combined ultrasonic-microwave extraction is 90-110W, and the microwave power is 280-320W.

8. The method for producing apple concentrate powder for preventing hair loss and promoting hair growth according to claim 1, characterized in that, In step S3, the macroporous resin is NKA-9.

9. The method for producing apple concentrate powder for preventing hair loss and promoting hair growth according to claim 1, characterized in that, The apple concentrate is apple polyphenols.

10. The application of apple concentrate powder prepared by any one of claims 1-9 in the fields of hair growth and hair loss prevention.

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