Traditional Chinese medicine formula for inhibiting repair of cancer cells and preparation method thereof

Abstract

The application relates to the technical field of traditional Chinese medicine, and discloses a traditional Chinese medicine formula for inhibiting repair of cancer cells and a preparation method, which is composed of white snake, gecko, scorpion, earthworm, Guang Sanqi, frankincense, myrrh, honeysuckle, pollen, Chuanlian, guanidine, Yunling, tuhufu, angelica, licorice, pinellia ternata, perilla seed and the like. The application can effectively inhibit proliferation of various tumor cells, induce tumor cell apoptosis, reduce the invasion and metastasis ability of tumor cells, can significantly inhibit tumor progression, and tumor lesions of part of patients can be obviously reduced or even disappeared.

Description

Technical Field

[0001] This invention relates to the field of traditional Chinese medicine technology, and in particular to a traditional Chinese medicine formula and preparation method for inhibiting and repairing cancer cells. Background Technology

[0002] Malignant tumors (cancer) are characterized by high incidence, high mortality, and poor prognosis. Currently, the main Western medical treatments for malignant tumors include surgery, radiotherapy, chemotherapy, targeted therapy, and immunotherapy. Surgery is only suitable for patients with early-stage localized tumors, and its effectiveness is limited for patients with mid-to-late-stage or metastatic tumors. While radiotherapy and chemotherapy can inhibit tumor progression to some extent, they have serious toxic side effects, such as bone marrow suppression, gastrointestinal reactions, liver and kidney damage, and hair loss, severely reducing patients' quality of life and easily leading to drug resistance.

[0003] For patients in the end stage of malignant tumors, cancer pain is the most common complication, with an incidence rate exceeding 80%, of which severe pain accounts for more than half. Currently available analgesics are mostly opioids, which have adverse reactions such as addiction, tolerance, constipation, and respiratory depression. Furthermore, they cannot treat the underlying cause of the tumor, only providing symptomatic relief. Simultaneously, cancer patients often experience loss of appetite, weight loss, cachexia, and a continuously weakened immune system, further accelerating tumor progression and creating a vicious cycle.

[0004] Therefore, a traditional Chinese medicine composition that inhibits tumor growth, kills cancer cells, and repairs lesions is proposed. Summary of the Invention

[0005] The purpose of this invention is to address the shortcomings of existing technologies by proposing a traditional Chinese medicine formula and preparation method for inhibiting and repairing cancer cells.

[0006] To achieve the above objectives, this invention provides a traditional Chinese medicine formula for inhibiting and repairing cancer cells: it is composed of white-flowered snake, gecko, blood scorpion, ground beetle, Panax notoginseng, frankincense, myrrh, honeysuckle, pollen, Coptis chinensis, alum, Poria cocos, Smilax glabra, Angelica sinensis, licorice, Pinellia ternata, and Perilla frutescens.

[0007] The formula uses gecko, white-flowered snake, gecko, blood scorpion, and ground beetle as the principal ingredients. Among them, gecko is salty in taste and neutral in nature, entering the lung and kidney meridians. It has the functions of tonifying the lungs and kidneys, regulating qi and relieving asthma, and strengthening yang and replenishing essence, while also having the effects of supporting the body's resistance and dispersing nodules. White-flowered snake is sweet and salty in taste and warm in nature, and is poisonous. It is good at dispelling wind and unblocking the meridians, attacking toxins and dispersing nodules. It has a good penetrating nature, reaching the internal organs and unblocking the meridians, breaking up masses and accumulations, attacking toxins and inhibiting cancer. Gecko is salty in taste and cold in nature, with the functions of dispelling wind and calming fright, detoxifying and dispersing nodules. Clinically, it is often used in the treatment of malignant tumors. It can inhibit tumor cell proliferation and promote tumor cell apoptosis; Blood scorpion tastes sweet and salty, and is neutral in nature. It enters the heart meridian and is good at promoting blood circulation, relieving pain, removing blood stasis, stopping bleeding, and promoting tissue regeneration. It can not only promote blood circulation, remove blood stasis, disperse nodules, and relieve pain, but also promote tissue regeneration and repair damaged lesions; Ground beetle tastes salty and is cold in nature. It has the function of breaking up blood stasis, removing blood stasis, and reconnecting tendons and bones. It is good at breaking up blood stasis, dissolving masses, softening hard masses, and dispersing nodules. It has a good effect on breaking up blood stasis and dispersing nodules for masses and accumulations.

[0008] Angelica sinensis, Pinellia ternata, and Perilla frutescens are used as adjuvant herbs. Angelica sinensis is sweet and pungent in taste, warm in nature, and enters the liver, heart, and spleen meridians. It is good at nourishing blood and promoting blood circulation, regulating menstruation and relieving pain, and moistening the intestines and promoting bowel movements. Pinellia ternata is pungent in taste, warm in nature, and toxic. It enters the spleen, stomach, and lung meridians. It is good at drying dampness and resolving phlegm, suppressing nausea and vomiting, and eliminating masses and nodules. It has a good effect on resolving phlegm and nodules in cases of phlegm-dampness accumulation. Perilla frutescens is pungent in taste, warm in nature, and enters the lung and large intestine meridians. It is good at lowering qi and resolving phlegm, relieving cough and asthma, and moistening the intestines and promoting bowel movements. It assists Pinellia ternata in drying dampness and resolving phlegm, eliminating masses and nodules, and at the same time, it can lower qi and relieve chest tightness, and improve the patient's digestive function.

[0009] Licorice is used as the guiding herb. Licorice is sweet in taste and neutral in nature. It enters the heart, lung, spleen, and stomach meridians. Its functions are to tonify the spleen and replenish qi, clear heat and detoxify, and harmonize the effects of other herbs. It can not only tonify the spleen and replenish qi, strengthen the body's resistance and consolidate the foundation, but also clear heat and detoxify, and alleviate the toxicity of poisonous herbs in the formula.

[0010] As a further description of the above technical solution: The composition includes the following components in parts by weight: gecko 25-35 parts, white-flowered snake 20-30 parts, gecko 45-55 parts, blood scorpion 45-55 parts, ground beetle 45-55 parts, Panax notoginseng 45-55 parts, frankincense 30-40 parts, myrrh 30-40 parts, honeysuckle 25-35 parts, pollen 45-55 parts, Coptis chinensis 45-55 parts, alum 20-30 parts, Poria cocos 45-55 parts, Smilax glabra 45-55 parts, Angelica sinensis 45-55 parts, licorice 45-55 parts, Pinellia ternata 45-55 parts, and Perilla frutescens 45-55 parts.

[0011] As a further description of the above technical solution: The components include the following parts by weight: gecko 28-32 parts, white-flowered snake 23-28 parts, gecko 48-53 parts, blood scorpion 48-53 parts, ground beetle 48-53 parts, Panax notoginseng 48-53 parts, frankincense 32-38 parts, myrrh 32-38 parts, honeysuckle 28-32 parts, pollen 48-53 parts, Coptis chinensis 48-53 parts, alum 23-28 parts, Poria cocos 48-53 parts, Smilax glabra 48-53 parts, Angelica sinensis 48-53 parts, licorice 48-53 parts, Pinellia ternata 48-53 parts, and Perilla frutescens 48-53 parts.

[0012] As a further description of the above technical solution: The ingredients include the following components by weight: 30 parts gecko, 25 parts white-flowered snake, 50 parts gecko, 50 parts blood scorpion, 50 parts ground beetle, 50 parts Panax notoginseng, 35 parts frankincense, 35 parts myrrh, 30 parts honeysuckle, 50 parts pollen, 50 parts Coptis chinensis, 25 parts alum, 50 parts Poria cocos, 50 parts Smilax glabra, 50 parts Angelica sinensis, 50 parts licorice, 50 parts Pinellia ternata, and 50 parts Perilla frutescens.

[0013] As a further description of the above technical solution: The traditional Chinese medicine composition is an oral preparation.

[0014] As a further description of the above technical solution: The oral preparation is a honey pill, and each honey pill weighs 8-12g.

[0015] As a further description of the above technical solution: The traditional Chinese medicine formula is used for the treatment and management of tumors and cancer pain.

[0016] In addition, a method for preparing a traditional Chinese medicine formula that inhibits and repairs cancer cells is also provided, including the following steps: S1: Weigh each raw material according to the prescription amount, and clean and dry them separately to obtain cleaned medicinal materials; S2: Crush each of the cleaned raw materials separately and pass them through an 80-120 mesh sieve to obtain fine powder of each raw material. Put all the fine powder into a mixing device and mix them evenly to obtain mixed powder. S3: Add excipients to the mixed powder to prepare the corresponding oral formulation, which is then dried.

[0017] As a further description of the above technical solution: In step S2, the powder is pulverized and passed through a 100-mesh sieve. The mixing time is 20-40 minutes until the powder color is uniform. In step S3, the oral preparation is a honey pill, the excipient is refined honey, and the amount of refined honey added is 100%-120% of the weight of the mixed powder. The refined honey is heated to 50-60℃, added to the mixed powder, stirred and mixed thoroughly, made into pills, shaped into pills, and dried at a low temperature of 40-60℃ to obtain the honey pill.

[0018] The present invention has the following beneficial effects: This invention effectively inhibits the proliferation of various tumor cells, induces tumor cell apoptosis, and reduces the invasive and metastatic abilities of tumor cells. After 1-2 months of treatment, it can significantly inhibit tumor progression, and in some patients, the tumor lesions can be significantly shrunk or even disappear. Detailed Implementation

[0019] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0020] To address the problems mentioned in the background art, this application presents a traditional Chinese medicine formula and preparation method for inhibiting and repairing cancer cells.

[0021] Example 1: Made from the following raw materials by weight: 30g gecko, 25g white-flowered snake, 50g gecko, 50g blood scorpion, 50g ground beetle, 50g Panax notoginseng, 35g frankincense, 35g myrrh, 30g honeysuckle, 50g pollen, 50g Coptis chinensis, 25g alum, 50g Poria cocos, 50g Smilax glabra, 50g Angelica sinensis, 50g licorice, 50g Pinellia ternata, and 50g Perilla frutescens.

[0022] The traditional Chinese medicine composition in this embodiment is a honey pill, each weighing 10g, and the preparation method is as follows: S1 Raw material pretreatment: Weigh each raw material according to the above prescription amount, and clean them according to the Chinese Pharmacopoeia 2025 edition of the Chinese Medicine Processing Specifications to remove impurities and non-medicinal parts. After washing, place them in a hot air circulating oven and dry them at a low temperature of 45℃, controlling the moisture content to ≤10% to obtain cleaned medicinal materials. S2 Pulverization and Mixing: Each of the cleaned raw materials is pulverized separately using a universal pulverizer and passed through a 100-mesh sieve to obtain fine powder of each raw material. All fine powders are put into a three-dimensional mixer and mixed for 30 minutes until the powder color is uniform and consistent, thus obtaining mixed powder. Samples are taken for testing, and the content uniformity meets the requirements. S3 Formulation: Take 110% of the weight of the mixed medicinal powder with refined honey, heat the refined honey to 55℃, slowly add it to the mixed medicinal powder, stir and mix thoroughly to form a medicinal ball with suitable softness and good plasticity, and make it into a pill using a fully automatic pill-making machine. After pill formation, place it in a hot air circulating oven and dry it at a low temperature of 50℃, controlling the moisture content to ≤12%, to obtain a honey pill product of 10g per pill.

[0023] Dosage: Take one pill orally, twice a day, morning and evening, with warm water. One month is one course of treatment.

[0024] Example 2: Made from the following raw materials by weight: 25g gecko, 20g white-flowered snake, 45g gecko, 45g blood scorpion, 45g ground beetle, 45g Panax notoginseng, 30g frankincense, 30g myrrh, 25g honeysuckle, 45g pollen, 45g Coptis chinensis, 20g alum, 45g Poria cocos, 45g Smilax glabra, 45g Angelica sinensis, 45g licorice, 45g Pinellia ternata, and 45g Perilla frutescens.

[0025] The preparation method is the same as in Example 1, and honey pills of 10g each are made.

[0026] Example 3: Made from the following raw materials by weight: 35g gecko, 30g white-flowered snake, 55g gecko, 55g blood scorpion, 55g ground beetle, 55g Panax notoginseng, 40g frankincense, 40g myrrh, 35g honeysuckle, 55g pollen, 55g Coptis chinensis, 30g alum, 55g Poria cocos, 55g Smilax glabra, 55g Angelica sinensis, 55g licorice, 55g Pinellia ternata, 55g Perilla frutescens.

[0027] The preparation method is the same as in Example 1, and honey pills of 10g each are made.

[0028] Results analysis: A total of 216 patients with malignant tumors admitted to the clinic were selected, including 118 males and 98 females, aged 32-78 years, with an average age of 56.4 years; including 42 cases of lung cancer, 38 cases of gastric cancer, 36 cases of liver cancer, 32 cases of colorectal cancer, 24 cases of breast cancer, 20 cases of esophageal cancer, and 24 cases of other malignant tumors; among them, 48 patients were in stage I-II, 168 patients were in stage III-IV, and 86 patients were in the terminal stage with severe cancer pain; All patients were treated with the honey pills prepared in Example 1 of this invention, taken orally, one pill each morning and evening with warm water, for one month as one course of treatment. Patients with malignant tumors received two consecutive courses of treatment. During the treatment period, patients discontinued other antitumor and antiviral drugs, received basic nutritional support, and their clinical symptoms, signs, imaging examinations, laboratory test results, and adverse reactions were recorded.

[0029] The criteria for evaluating the efficacy of tumor treatment were established by observing changes in tumor lesions (RECIST criteria), cancer pain relief (VAS score), weight changes, quality of life score (KPS score), blood routine tests, liver and kidney function, and other safety indicators. Complete remission (CR): The tumor lesions completely disappear and last for more than 4 weeks; Partial response (PR): The sum of the largest diameters of the tumor lesions shrinks by ≥30% for more than 4 weeks; Stable disease (SD): The sum of the largest diameters of the tumor lesions has shrunk by less than 30% or increased by less than 20%; Disease progression (PD): The sum of the largest diameters of tumor lesions increases by ≥20%, or new lesions appear.

[0030] Overall response rate = (CR + PR) / total number of cases × 100%, disease control rate = (CR + PR + SD) / total number of cases × 100%.

[0031] Treatment results: Of the 216 patients with malignant tumors, after two cycles of treatment, 22 achieved complete remission (CR), 126 achieved partial remission (PR), 54 achieved stable disease (SD), and 14 achieved progressive disease (PD), with an overall response rate of 68.52% and a disease control rate of 93.52%.

[0032] Of the 86 end-stage patients with severe cancer pain, 58 showed significant pain relief, 24 showed relief, and 4 showed no relief, with a total effective rate of 95.35%. Patients' analgesic drug dosage was significantly reduced, and their sleep and quality of life were significantly improved.

[0033] After treatment, all patients experienced varying degrees of improvement in appetite. Among them, 182 patients showed improvement in appetite after 3 days of treatment. After 1 month of treatment, the average weight gain was 2.36 kg, the average KPS score improved by 22.6 points, and the patients' quality of life was significantly improved.

[0034] In terms of safety, no significant abnormalities were observed in blood routine tests, liver and kidney function during the treatment of all patients, no obvious toxic side effects were observed, and patients tolerated the treatment well.

[0035] The results above show that it has good therapeutic effects on a variety of malignant tumors, can effectively inhibit tumor progression, shrink tumor lesions, relieve cancer pain, improve patients' appetite and nutritional status, improve patients' quality of life, and is safe with no obvious toxic side effects.

[0036] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine formula for inhibiting and repairing cancer cells, characterized in that, It is made from a combination of white-flowered snake, gecko, blood scorpion, ground beetle, Panax notoginseng, frankincense, myrrh, honeysuckle, pollen, Coptis chinensis, alum, Poria cocos, Smilax glabra, Angelica sinensis, licorice, Pinellia ternata, and Perilla frutescens.

2. The traditional Chinese medicine formula for inhibiting and repairing cancer cells according to claim 1, characterized in that, The composition includes the following components in parts by weight: gecko 25-35 parts, white-flowered snake 20-30 parts, gecko 45-55 parts, blood scorpion 45-55 parts, ground beetle 45-55 parts, Panax notoginseng 45-55 parts, frankincense 30-40 parts, myrrh 30-40 parts, honeysuckle 25-35 parts, pollen 45-55 parts, Coptis chinensis 45-55 parts, alum 20-30 parts, Poria cocos 45-55 parts, Smilax glabra 45-55 parts, Angelica sinensis 45-55 parts, licorice 45-55 parts, Pinellia ternata 45-55 parts, and Perilla frutescens 45-55 parts.

3. The traditional Chinese medicine formula for inhibiting and repairing cancer cells according to claim 2, characterized in that, The components include the following parts by weight: gecko 28-32 parts, white-flowered snake 23-28 parts, gecko 48-53 parts, blood scorpion 48-53 parts, ground beetle 48-53 parts, Panax notoginseng 48-53 parts, frankincense 32-38 parts, myrrh 32-38 parts, honeysuckle 28-32 parts, pollen 48-53 parts, Coptis chinensis 48-53 parts, alum 23-28 parts, Poria cocos 48-53 parts, Smilax glabra 48-53 parts, Angelica sinensis 48-53 parts, licorice 48-53 parts, Pinellia ternata 48-53 parts, and Perilla frutescens 48-53 parts.

4. The traditional Chinese medicine formula for inhibiting and repairing cancer cells according to claim 3, characterized in that, The ingredients include the following components by weight: 30 parts gecko, 25 parts white-flowered snake, 50 parts gecko, 50 parts blood scorpion, 50 parts ground beetle, 50 parts Panax notoginseng, 35 parts frankincense, 35 parts myrrh, 30 parts honeysuckle, 50 parts pollen, 50 parts Coptis chinensis, 25 parts alum, 50 parts Poria cocos, 50 parts Smilax glabra, 50 parts Angelica sinensis, 50 parts licorice, 50 parts Pinellia ternata, and 50 parts Perilla frutescens.

5. The traditional Chinese medicine formula for inhibiting and repairing cancer cells according to claim 4, characterized in that, The traditional Chinese medicine composition is an oral preparation.

6. The traditional Chinese medicine formula for inhibiting and repairing cancer cells according to claim 5, characterized in that, The oral preparation is a honey pill, and each honey pill weighs 8-12g.

7. The traditional Chinese medicine formula for inhibiting and repairing cancer cells according to claim 6, characterized in that, The traditional Chinese medicine formula is used for the treatment and management of tumors and cancer pain.

8. A method for preparing a traditional Chinese medicine formula that inhibits and repairs cancer cells, characterized in that, The method for preparing the traditional Chinese medicine formula according to any one of claims 1-4 is characterized by comprising the following steps: S1: Weigh each raw material according to the prescription amount, and clean and dry them separately to obtain cleaned medicinal materials; S2: Crush each of the cleaned raw materials separately and pass them through an 80-120 mesh sieve to obtain fine powder of each raw material. Put all the fine powder into a mixing device and mix them evenly to obtain mixed powder. S3: Add excipients to the mixed powder to prepare the corresponding oral formulation, which is then dried.

9. The traditional Chinese medicine formula and preparation method for inhibiting and repairing cancer cells according to claim 8, characterized in that, In step S2, the powder is pulverized and passed through a 100-mesh sieve. The mixing time is 20-40 minutes until the powder color is uniform. In step S3, the oral preparation is a honey pill, the excipient is refined honey, and the amount of refined honey added is 100%-120% of the weight of the mixed powder. The refined honey is heated to 50-60℃, added to the mixed powder, stirred and mixed thoroughly, made into pills, shaped into pills, and dried at a low temperature of 40-60℃ to obtain the honey pill.