Preparation method of composite bacillus bio-organic fertilizer

Abstract

This invention provides a method for preparing compound Bacillus bio-organic fertilizer, relating to the field of compound Bacillus preparation technology. The method includes a compound Bacillus: the compound Bacillus is composed of the following percentages of raw materials: 10%-25% methyltrophic Bacillus, 6%-25% Bacillus licheniformis, 10%-30% Bacillus amyloliquefaciens, 15%-35% Bacillus subtilis, and 6%-16% of actinomycetes and Trichoderma functional bacteria mixed in equal proportions; and a compound Bacillus bio-organic fertilizer, which is composed of the following parts by weight of raw materials: 3-6 parts compound Bacillus, 10-25 parts superphosphate, and 100-150 parts agricultural waste. By utilizing the functional microbial agent of compound Bacillus composed of methyltrophic Bacillus, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus subtilis, actinomycetes, and Trichoderma, various organic matter in the soil is better and faster decomposed into nutrients that plants can absorb, promoting plant growth.

Description

Technical Field

[0001] This invention relates to the field of compound Bacillus preparation technology, specifically a method for preparing compound Bacillus bio-organic fertilizer. Background Technology

[0002] Plant straw microbial fermentation is a method of converting plant straw into organic fertilizer or feed through microbial fermentation. This method not only effectively treats agricultural waste but also improves soil fertility and structure, while reducing production costs and increasing economic benefits.

[0003] The functional bacteria in Bacillus bio-organic fertilizers currently used in the market are mainly Bacillus subtilis, which has good application effects. However, the functional bacteria are singular, and there is still room for improvement in the decomposition efficiency of complex organic matter in the soil. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a method for preparing compound Bacillus bio-organic fertilizer, which solves the problem that existing technologies cannot effectively ferment agricultural waste to obtain organic fertilizer due to the limited number of functional bacteria.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a compound Bacillus bio-organic fertilizer:

[0006] Includes a complex of Bacillus bacteria: the complex of Bacillus bacteria is composed of the following percentages of raw materials:

[0007] Methyltrophic Bacillus 10%-25%, Bacillus licheniformis 6%-25%, Bacillus amyloliquefaciens 10%-30%, Bacillus subtilis 15%-35%, and Actinomycetes and Trichoderma functional bacteria mixed in equal proportions totaling 6%-16%;

[0008] This includes a compound Bacillus bio-organic fertilizer, which is composed of the following raw materials in parts by weight:

[0009] 3-6 parts of compound Bacillus, 10-25 parts of superphosphate, and 100-150 parts of agricultural waste.

[0010] Preferably, the methyltrophic Bacillus culture method is as follows: TSA medium is prepared and sterilized according to conventional methods, and cultured at a temperature of 30-37°C and a pH of 6.5-7.5 with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria.

[0011] The Bacillus licheniformis culture method is as follows: TSA medium is prepared and sterilized according to conventional methods, and cultured at a temperature of 30-37°C, pH of 6.5-7.5, with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria.

[0012] The method for culturing Bacillus amyloliquefaciens is as follows: TSA medium is prepared and sterilized according to conventional methods. The medium is then cultured at a temperature of 30-37°C and a pH of 6.5-7.5 with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria.

[0013] The method for culturing Bacillus subtilis is as follows: TSA medium is prepared and sterilized according to conventional methods. The medium is then cultured at a temperature of 30-37°C and a pH of 6.5-7.5 with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria.

[0014] Preferably, the compound Bacillus bio-organic fertilizer is composed of the following raw materials in parts by weight: 5 parts compound Bacillus, 10 parts superphosphate, and 100 parts agricultural waste.

[0015] Preferably, the agricultural waste is one or a mixture of more than one of the following: rice straw, wheat straw, corn cobs, vegetable scraps, branches, fallen leaves, livestock and poultry excrement, livestock by-products, fruit pomace, rotten fruits and vegetables, and kitchen waste.

[0016] Preferably, a method for preparing compound Bacillus bio-organic fertilizer includes the following steps:

[0017] Step 1, composting and fermentation: Mix agricultural waste and superphosphate with water and compost and ferment. Measure the compost temperature. If the temperature in the center of the compost pile is higher than 55 degrees Celsius, turn the pile with a turning machine to increase oxygen. The composting and fermentation time is 10-15 days.

[0018] Step 2, composting and fermentation: The fertilizer piled up for fermentation in Step 1 is composted and fermented at 50-60 degrees Celsius for 20-35 days to obtain composted raw materials;

[0019] Step 3, Nutrient Balance: Mix compound Bacillus with the composted raw materials from Step 2. After mixing, culture at 30-45 degrees Celsius for 20-40 days. Then, conduct nutrient testing and add nitrogen, phosphorus, and potassium to adjust the nutrient content to obtain organic fertilizer.

[0020] Step 4: Packaging: Pack the organic fertilizer into bags, each weighing 15-30 kg, to obtain the finished organic fertilizer product.

[0021] Preferably, the humidity of the compost pile in step one is 80%-90%, and the temperature of the compost pile fermentation is 50-55 degrees Celsius.

[0022] This invention provides a method for preparing compound Bacillus bio-organic fertilizer. It has the following beneficial effects:

[0023] This invention utilizes a compound Bacillus functional agent composed of methyl-nutritive Bacillus, Bacillus licheniformis, Bacillus amyloliquefaciens, Bacillus subtilis, actinomycetes, and Trichoderma to better and faster decompose various organic matter in the soil into nutrients that plants can absorb, thus promoting plant growth. Through fermentation and combined with the microbial agent, agricultural waste can be fully utilized and decomposed into nutrient-rich organic fertilizer, achieving excellent organic fertilizer preparation that is beneficial to plant growth. Attached Figure Description

[0024] Figure 1 This is a flowchart illustrating the preparation process of the present invention. Detailed Implementation

[0025] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0026] Example:

[0027] like Figure 1 As shown, this embodiment of the invention provides a compound Bacillus bio-organic fertilizer:

[0028] Includes a complex of Bacillus bacteria: the complex of Bacillus bacteria is composed of the following percentages of raw materials:

[0029] Methyltrophic Bacillus 15%, Bacillus licheniformis 10%, Bacillus amyloliquefaciens 20%, Bacillus subtilis 30%, Actinomycetes 5%, and Trichoderma functional bacteria 5%;

[0030] This includes a compound Bacillus bio-organic fertilizer, which is composed of the following raw materials in parts by weight:

[0031] 5 parts of compound Bacillus, 12 parts of superphosphate, and 100 parts of agricultural waste;

[0032] When the bio-organic fertilizer containing the compound functional bacteria of this invention was cultivated under the same conditions as Bacillus subtilis organic fertilizer for 20-40 days, the decomposition of crude fiber in the organic fertilizer increased by 10.2%-15.8%, crude protein by 5.62%-7.8%, crude fat by 15.2%-25.5%, and lignin by 20.2%-35.7%.

[0033] Bio-organic fertilizer using the compound functional bacteria of this invention and Bacillus subtilis organic fertilizer were applied to the soil under the same conditions. 30 days later, the changes in organic nutrients in the soil were measured. The changes were: crude fiber decomposition increased by 6.8.2%, crude protein decomposition increased by 6.6%, crude fat decomposition increased by 18.2%, and lignin decomposition increased by 22.0%. Good experimental results were achieved.

[0034] The methyltrophic Bacillus culture method is as follows: TSA medium is prepared and sterilized according to conventional methods, and cultured at a temperature of 30-37°C, pH of 6.5-7.5, with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria.

[0035] The Bacillus licheniformis culture method is as follows: TSA medium is prepared and sterilized according to conventional methods, and cultured at a temperature of 30-37°C, pH of 6.5-7.5, with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria.

[0036] The method for culturing Bacillus amyloliquefaciens is as follows: TSA medium is prepared and sterilized according to conventional methods. The medium is then cultured at a temperature of 30-37°C and a pH of 6.5-7.5 with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria.

[0037] The method for culturing Bacillus subtilis is as follows: TSA medium is prepared and sterilized according to conventional methods. The medium is then cultured at a temperature of 30-37°C and a pH of 6.5-7.5 with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria.

[0038] The compound Bacillus bio-organic fertilizer is composed of the following raw materials in parts by weight: 5 parts compound Bacillus, 10 parts superphosphate, and 100 parts agricultural waste.

[0039] Multiple single strains are mixed in a certain proportion to obtain compound Bacillus, which can be used to decompose and ferment agricultural waste.

[0040] The agricultural waste is one or a mixture of more than one of the following: rice straw, wheat straw, corn cobs, vegetable scraps, tree branches, fallen leaves, livestock and poultry excrement, livestock by-products, fruit residue, rotten fruits and vegetables, and kitchen waste.

[0041] Example 2:

[0042] Reference Figure 1 As shown in the figure, this invention provides a method for preparing compound Bacillus bio-organic fertilizer, including the following steps:

[0043] Step 1, composting and fermentation: Mix agricultural waste and superphosphate with water and compost and ferment. Measure the compost temperature. If the temperature in the center of the compost pile is higher than 55 degrees Celsius, turn the pile with a turning machine to increase oxygen. The composting and fermentation time is 10 days.

[0044] Step 2, composting and fermentation: The fertilizer piled up for fermentation in Step 1 is composted and fermented at 50 degrees Celsius for 30 days to obtain composted raw materials;

[0045] Step 3, Nutrient Balance: Mix compound Bacillus with the composted raw materials from Step 2. After mixing, culture at 45 degrees Celsius for 40 days. Then, conduct nutrient testing and add nitrogen, phosphorus, and potassium to adjust the nutrient content to obtain organic fertilizer.

[0046] Step 4: Packaging: Pack the organic fertilizer into bags, each weighing 15-30 kg, to obtain the finished organic fertilizer product.

[0047] In step one, the humidity of the compost pile for fermentation is 90%, and the temperature for fermentation is 55 degrees Celsius.

[0048] The full decomposition of organic matter can break down the nutrients in agricultural waste, which helps increase the organic matter in the soil. It can also increase beneficial bacteria in the soil and promote the root growth of crops. The roots can effectively absorb the elements in the organic matter, promote crop growth, and avoid the problem of poor decomposition of organic fertilizer in the field.

[0049] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A compound Bacillus bio-organic fertilizer, characterized in that: Includes a complex of Bacillus bacteria: the complex of Bacillus bacteria is composed of the following percentages of raw materials: Methyltrophic Bacillus 10%-25%, Bacillus licheniformis 6%-25%, Bacillus amyloliquefaciens 10%-30%, Bacillus subtilis 15%-35%, and Actinomycetes and Trichoderma functional bacteria mixed in equal proportions totaling 6%-16%; This includes a compound Bacillus bio-organic fertilizer, which is composed of the following raw materials in parts by weight: 3-6 parts of compound Bacillus, 10-25 parts of superphosphate, and 100-150 parts of agricultural waste.

2. The compound Bacillus bio-organic fertilizer according to claim 1, characterized in that: The methyltrophic Bacillus culture method is as follows: TSA medium is prepared and sterilized according to conventional methods, and cultured at a temperature of 30-37°C, pH of 6.5-7.5, with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria. The Bacillus licheniformis culture method is as follows: TSA medium is prepared and sterilized according to conventional methods, and cultured at a temperature of 30-37°C, pH of 6.5-7.5, with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria. The method for culturing Bacillus amyloliquefaciens is as follows: TSA medium is prepared and sterilized according to conventional methods. The medium is then cultured at a temperature of 30-37°C and a pH of 6.5-7.5 with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria. The method for culturing Bacillus subtilis is as follows: TSA medium is prepared and sterilized according to conventional methods. The medium is then cultured at a temperature of 30-37°C and a pH of 6.5-7.5 with aeration for 24 hours. The turbidity of the bacterial solution is observed to determine the growth status and obtain single bacteria.

3. The compound Bacillus bio-organic fertilizer according to claim 1, characterized in that: The compound Bacillus bio-organic fertilizer is composed of the following raw materials in parts by weight: 5 parts compound Bacillus, 10 parts superphosphate, and 100 parts agricultural waste.

4. The compound Bacillus bio-organic fertilizer according to claim 1, characterized in that: The agricultural waste is one or a mixture of more than one of the following: rice straw, wheat straw, corn cobs, vegetable scraps, tree branches, fallen leaves, livestock and poultry excrement, livestock by-products, fruit residue, rotten fruits and vegetables, and kitchen waste.

5. A method for preparing a compound Bacillus bio-organic fertilizer according to any one of claims 1-4, characterized in that: Includes the following steps: Step 1, composting and fermentation: Mix agricultural waste and superphosphate with water and compost and ferment. Measure the compost temperature. If the temperature in the center of the compost pile is higher than 55 degrees Celsius, turn the pile with a turning machine to increase oxygen. The composting and fermentation time is 10-15 days. Step 2, composting and fermentation: The fertilizer piled up for fermentation in Step 1 is composted and fermented at 50-60 degrees Celsius for 20-35 days to obtain composted raw materials; Step 3, Nutrient Balance: Mix compound Bacillus with the composted raw materials from Step 2. After mixing, culture at 30-45 degrees Celsius for 20-40 days. Then, conduct nutrient testing and add nitrogen, phosphorus, and potassium to adjust the nutrient content to obtain organic fertilizer. Step 4: Packaging: Pack the organic fertilizer into bags, each weighing 15-30 kg, to obtain the finished organic fertilizer product.

6. The method for preparing a compound Bacillus bio-organic fertilizer according to claim 5, characterized in that: In step one, the humidity of the compost pile for fermentation is 80%-90%, and the temperature for fermentation is 50-55 degrees Celsius.

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