A spycatcher immobilization mutant, spyfixer, and uses thereof
CN122167542APending Publication Date: 2026-06-09SOUTH CHINA UNIV OF TECH
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- SOUTH CHINA UNIV OF TECH
- Filing Date
- 2024-12-06
- Publication Date
- 2026-06-09
AI Technical Summary
Technical Problem
The existing SpyCatcher exhibits low immobilization efficiency and low activity on various solid supports, limiting its application and making it difficult to meet the needs of protein immobilization.
Method used
A SpyCatcher immobilization mutant, SpyFixer, was developed by mutating the 77th amino acid position to glutamic acid (E) to achieve covalent bonding with epoxy or carboxylic acid groups on the carrier surface for protein immobilization.
Benefits of technology
It improves the immobilization efficiency and activity of SpyCatcher on various carrier surfaces, expands its application range, and achieves highly selective and rapid protein immobilization, suitable for fiber optic biosensors, epoxy agarose resin and epoxy acrylate resin.
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Smart Images
Figure CN122167542A_ABST
Abstract
The application discloses a SpyCatcher immobilized mutant SpyFixer and application thereof. The mutant is obtained by introducing an A77E mutation site based on SpyDock. The mutant is respectively immobilized on a fiber biosensor, a hydrophilic epoxy agarose resin and a hydrophobic epoxy acrylate resin to obtain different carriers modified by SpyFixer, and then target proteins containing SpyTag are respectively immobilized on the different carriers modified by SpyFixer through Spy chemistry, and are used for protein interaction detection, immune detection, purification and immobilization. The mutant can be effectively immobilized on various types of carriers, and the immobilization efficiency and the binding activity after immobilization are superior to those of SpyCatcher and SpyCatcher003, and the application range is wider. Moreover, the proteins immobilized on the carriers modified by SpyFixer can be repeatedly used, and have potential industrial utilization value.
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