Method for extracting menotropins from the urine of postmenopausal women

Abstract

The present application relates to the technical field of biotechnology, and discloses a method for extracting menotropin from urine of postmenopausal women, which comprises the following steps: S1, filtering the urine of postmenopausal women, adjusting the pH value to 4-4.5, adding modified kaolin and mixing, adding perlite and mixing, standing, filtering, and drying to obtain kaolin adsorbate; S2, adding desorption liquid to the kaolin adsorbate, performing secondary desorption, combining the filtrates of the two times, adjusting the pH value to 8.2-8.7, standing, adding perlite and mixing, standing, filtering, ultrafiltering to obtain concentrated liquid, adjusting the pH value of the concentrated liquid to 5-5.5, adding acetone and mixing, standing, suction filtering, dehydrating with acetone, screening, and drying to obtain menotropin; the modified kaolin is obtained by compounding kaolin with chitosan first and then modifying the compound with amino silane. Through the above technical scheme, the problems of low extraction yield and purity of menotropin in the related art are solved.

Description

Technical Field

[0001] This invention relates to the field of biotechnology, and more specifically, to a method for extracting urinary gonadotropins from the urine of postmenopausal women. Background Technology

[0002] Urinary gonadotropin is a glycoprotein gonadotropin secreted by the anterior pituitary gland in the human brain. It is generally extracted and purified from the urine of postmenopausal women. Clinically, it is mainly used to treat infertility in women caused by functional anovulation. It can also improve sperm count or motility in men caused by low hormone levels. In addition, it can be used to regulate amenorrhea, menstrual disorders, and sexual dysfunction in both men and women.

[0003] Existing technologies for extracting urinary gonadotropins mostly employ kaolin adsorption. However, the effective active sites on the surface of kaolin are insufficient, making it difficult to achieve better adsorption of urinary gonadotropins in the urine of postmenopausal women. Consequently, the extraction yield and purity of urinary gonadotropins from the urine of postmenopausal women are low. Therefore, it is of great significance to develop an extraction method that can obtain high yield and high purity of urinary gonadotropins. Summary of the Invention

[0004] This invention proposes a method for extracting urinary gonadotropins from the urine of postmenopausal women, which solves the problems of low extraction yield and purity of urinary gonadotropins in related technologies.

[0005] The technical solution of the present invention is as follows: This invention proposes a method for extracting urinary gonadotropins from the urine of postmenopausal women, the method comprising the following steps: S1. After filtering the urine of postmenopausal women through a filter screen, the pH value is adjusted to 4-4.5, and modified kaolin is added and mixed to obtain mixture I; S2. After adding perlite I to mixed liquid I and mixing, let stand, discard the clear liquid on the top layer, filter through a plate and frame filter, wash and dry to obtain kaolin adsorbent. S3. Add desorption solution I to the kaolin adsorbent, adjust the pH value to 11~11.5 and mix. Filter the mixture through a plate and frame filter to obtain a first filter cake and a first filtrate. Add desorption solution II to the first filter cake, adjust the pH value to 11~11.5 and mix. Filter the mixture through a plate and frame filter to obtain a second filter cake and a second filtrate. Combine the first filtrate and the second filtrate to obtain mixture II. S4. After adjusting the pH of the mixture II to 8.2~8.7, let it stand, add perlite II and mix, let it stand, filter through a plate and frame filter to obtain the third filtrate, and then ultrafilter and concentrate the third filtrate to obtain the concentrated solution. S5. After adjusting the pH of the concentrate to 5-5.5, add acetone and mix. Let stand, filter, and obtain the precipitate. After dehydrating the precipitate with acetone, sieve and dry to obtain urinary gonadotropin. The modified kaolin is obtained by first combining kaolin with chitosan, and then modifying it with amino silanes.

[0006] As a further technical solution, the pH value of the urine from the postmenopausal woman is 5.4~6.2, and the density of the urine from the postmenopausal woman is 1.01~1.018 g / cm³. 3 The urine of the postmenopausal women was clear and free of turbidity.

[0007] As a further technical solution, the mass ratio of kaolin, chitosan and aminosilane is 100:20:5~8, preferably 50:10:3.

[0008] As a further technical solution, the preparation method of the modified kaolin is as follows: A1. After dispersing chitosan evenly in solvent I, add kaolin and mix to obtain premixed solution I; A2. Liquid paraffin and Span 80 are added to premixed liquid I and mixed. Glutaraldehyde is added to react with the mixture. After washing, drying and pulverizing, kaolin composite chitosan is obtained. A3. After the amino silane is evenly dispersed in solvent II, kaolin and chitosan composite are added and mixed. After filtration and drying, modified kaolin is obtained.

[0009] As a further technical solution, solvent I is a 2wt% aqueous solution of acetic acid.

[0010] As a further technical solution, in step A2, the mixing temperature is 30~40℃ and the mixing time is 1~2h.

[0011] As a further technical solution, in step A2, the reaction temperature is 50~60℃ and the reaction time is 1~2h.

[0012] As a further technical solution, solvent II is composed of anhydrous ethanol and water in a mass ratio of 4:1.

[0013] As a further technical solution, in step A3, the mixing temperature is 30~40℃ and the mixing time is 40~60min.

[0014] As a further technical solution, the amino silanes include 3-anilinopropyltrimethoxysilane and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane.

[0015] This invention utilizes a combination of 3-anilinepropyltrimethoxysilane and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane as amino silanes to synergistically modify kaolin, thereby improving the yield and purity of urinary gonadotropins. Specifically, the silane groups of 3-anilinepropyltrimethoxysilane and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane can form covalent bonds with the active groups on the surface of the kaolin-chitosan composite, effectively reducing the aggregation of the kaolin-chitosan composite. Furthermore, the benzene ring structure in 3-anilinepropyltrimethoxysilane can enhance the adsorption of aromatic amino acid residues in the urinary gonadotropin molecule through π-π interactions.

[0016] As a further technical solution, the mass ratio of 3-anilinopropyltrimethoxysilane to N-(2-aminoethyl)-3-aminopropyltrimethoxysilane is 2~3:1, preferably 2.5:1.

[0017] As a further technical solution, in step S1, the mixing time is 20~40 minutes.

[0018] As a further technical solution, in step S2, the mixing time is 2~4 hours.

[0019] As a further technical solution, in step S3, the desorption solution is ammonia.

[0020] As a further technical solution, in step S5, the mixing time is 15~25 minutes.

[0021] As a further technical solution, in step S5, the settling time is 8~10h.

[0022] The working principle and beneficial effects of this invention are as follows: This invention proposes a method for extracting urinary gonadotropins from the urine of postmenopausal women, wherein the addition of modified kaolin improves the yield and purity of urinary gonadotropins. The modified kaolin is obtained by first compounding kaolin with chitosan, and then modifying it with aminosilanes. The compounding of chitosan and kaolin increases the adsorption active sites, enhancing the adsorption capacity for urinary gonadotropins, and also forms a stable composite structure through cross-linking. However, the resulting composite particles are prone to aggregation in the urine system. Therefore, aminosilanes are further introduced for modification, improving the dispersibility of the modified kaolin during the extraction process, thereby increasing the yield and purity of urinary gonadotropins. Detailed Implementation

[0023] The technical solutions of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative effort are within the scope of protection of the present invention.

[0024] In the following examples and comparative examples: The ammonia solution is 25 wt% ammonia solution; The sodium hydroxide is a 1 mol / L aqueous solution of sodium hydroxide; Chitosan, average particle size 100 mesh, model: JRX-KJT, manufacturer: Taiyuan Jiruixin Biotechnology Co., Ltd. Kaolin, average particle size: 5μm; Perlite, average particle size: 80 mesh; The liquid paraffin is 68# liquid paraffin.

[0025] Example 1 A method for extracting urinary gonadotropins from the urine of postmenopausal women includes the following steps: S1. The urine of postmenopausal women (the pH of postmenopausal women's urine is 5.8, and the density is 1.014 g / cm³) 3 After being filtered through a filter screen, the urine of postmenopausal women (hereinafter referred to as urine) was obtained. Acetic acid (50wt% aqueous acetic acid solution) was added to adjust the pH of the urine to 4. Modified kaolin (addition amount of 5.5kg / ton of urine) was added and mixed for 20 minutes to obtain mixture I. S2. Add perlite (2.0 kg / ton of urine) to mixture I and mix for 2 hours. Let stand for 15 hours, discard the clear liquid on top, and filter the remaining mixture through a plate and frame filter to obtain a filter cake. Wash the filter cake with acetic acid (15 L / ton of urine, pH 4.2) and dry it by compression and air blowing for 30 minutes to obtain kaolin adsorbent. S3. Add 300L of ammonia water to the kaolin adsorbent, adjust the pH value to 11, mix for 2 hours, filter by plate and frame to obtain the first filter cake and the first filtrate. Add ammonia water to the first filter cake, adjust the pH value to 11, mix for 1 hour, filter by plate and frame to obtain the second filter cake and the second filtrate. Combine the first filtrate and the second filtrate to obtain mixture II. S4. After adjusting the pH value to 8.2 by adding acetic acid to mixture II and letting it stand for 15 hours, add perlite (addition amount is 0.5 kg / ton of urine) and mix. After mixing, filter through plate and frame filter, collect the filtrate, and then ultrafilter and concentrate the filtrate to 10% of the liquid volume to obtain concentrated solution. S5. After adjusting the pH of the concentrate to 5 with acetic acid, add acetone (the volume ratio of concentrate to acetone is 1:2) and mix for 15 minutes. Let stand for 8 hours, take the precipitate, filter it, dehydrate it with acetone, dry it, pass the precipitate through a 40-mesh sieve, and dry it at -0.09 MPa and 25℃ for 8 hours to obtain urinary gonadotropin. The preparation method of modified kaolin is as follows: A1. After dispersing chitosan evenly in a 2wt% acetic acid aqueous solution (the mass-volume ratio of chitosan to 2wt% acetic acid aqueous solution is 1g:50mL), kaolin is added and mixed for 2h to obtain premix solution I. A2. Liquid paraffin and Span 80 were added to premixed solution I and mixed at 30°C for 2 hours. Then, 25 wt% glutaraldehyde aqueous solution was added and reacted at 50°C for 2 hours. After washing with petroleum ether and anhydrous ethanol, the mixture was dried and pulverized to obtain kaolin composite chitosan with a particle size of 20 μm. The volume ratio of 2 wt% acetic acid aqueous solution, liquid paraffin, Span 80 and 25 wt% glutaraldehyde aqueous solution was 25 mL:100 mL:4 mL:3 mL. A3. After uniformly dispersing aminosilanes in an ethanol solution (composed of anhydrous ethanol and water in a mass ratio of 4:1), add kaolin composite chitosan and mix at 30°C for 60 minutes. After filtration and drying, modified kaolin is obtained. The mass-to-volume ratio of kaolin to ethanol solution is 1 g: 30 mL, the amino silane is 3-phenylaminopropyltrimethoxysilane, and the mass ratio of kaolin, chitosan and amino silane is 20:4:1. Results: The yield of urinary gonadotropin was 87.9%, and the purity was 94.3%.

[0026] Example 2 A method for extracting urinary gonadotropins from the urine of postmenopausal women, the method comprising the following steps: S1. The urine of postmenopausal women (the pH of postmenopausal women's urine is 5.8, and the density is 1.014 g / cm³) 3 The urine (which is clear and free of turbidity) is filtered through a filter to obtain the treated postmenopausal urine (hereinafter referred to as urine). Acetic acid (50wt% aqueous acetic acid solution) is added to adjust the pH of the urine to 4.2. Modified kaolin (addition amount of 5.5kg / ton of urine) is added and mixed for 30 minutes to obtain mixture I. S2. Add perlite (2.0 kg / ton of urine) to mixture I and mix for 3 hours. Let stand for 18 hours, discard the clear liquid on top, and filter the remaining mixture through a plate and frame filter to obtain a filter cake. Wash the filter cake with acetic acid (15 L / ton of urine, pH 4.2) and dry it by compression and air blowing for 30 minutes to obtain kaolin adsorbent. S3. Add 300L of ammonia water to the kaolin adsorbent, adjust the pH to 11.2, mix for 2.3h, filter by plate and frame to obtain the first filter cake and the first filtrate. Add ammonia water to the first filter cake, adjust the pH to 11.2, mix for 1.5h, filter by plate and frame to obtain the second filter cake and the second filtrate. Combine the first filtrate and the second filtrate to obtain mixture II. S4. After adjusting the pH to 8.5 by adding acetic acid to mixture II, let it stand for 17 hours. Then add perlite (0.5 kg / ton of urine) and mix. After filtration through a plate and frame filter, collect the filtrate. Then, ultrafilter and concentrate the filtrate to 10% of the liquid volume. The concentrated solution is obtained by ultrafiltration. S5. After adjusting the pH of the concentrate to 5.3 with acetic acid, add acetone (volume ratio of concentrate to acetone is 1:2) and mix for 20 minutes. Let stand for 9 hours, take the precipitate, filter it, dehydrate it with acetone, dry it, pass the precipitate through a 40-mesh sieve, and dry it at 0 MPa and 25℃ for 9 hours to obtain urinary gonadotropin. The preparation method of modified kaolin is as follows: A1. After dispersing chitosan evenly in a 2wt% acetic acid aqueous solution (the mass-volume ratio of chitosan to 2wt% acetic acid aqueous solution is 1g:50mL), kaolin is added and mixed for 2.5h to obtain premix solution I. A2. Liquid paraffin and Span 80 were added to premixed solution I and mixed at 35°C for 1.5 h. Then, 25 wt% glutaraldehyde aqueous solution was added and reacted at 55°C for 1.5 h. After washing with petroleum ether and anhydrous ethanol, the mixture was dried and pulverized to obtain kaolin composite chitosan with a particle size of 20 μm. The volume ratio of 2 wt% acetic acid aqueous solution, liquid paraffin, Span 80 and 25 wt% glutaraldehyde aqueous solution was 25 mL:100 mL:4 mL:3 mL. A3. After uniformly dispersing aminosilanes in an ethanol solution (composed of anhydrous ethanol and water in a mass ratio of 4:1), add kaolin composite chitosan and mix at 35°C for 45 min. After filtration and drying, modified kaolin is obtained. The mass-to-volume ratio of kaolin to ethanol solution is 1 g: 30 mL, the amino silane is 3-phenylaminopropyltrimethoxysilane, and the mass ratio of kaolin, chitosan and amino silane is 20:4:1. Results: The yield of urinary gonadotropin was 88.3%, and the purity was 95.1%.

[0027] Example 3 A method for extracting urinary gonadotropins from the urine of postmenopausal women, the method comprising the following steps: S1. The urine of postmenopausal women (the pH of postmenopausal women's urine is 5.8, and the density is 1.014 g / cm³) 3After being filtered through a filter screen, the urine of postmenopausal women (hereinafter referred to as urine) was treated. Acetic acid (50wt% aqueous acetic acid solution) was added to adjust the pH of the urine to 4.5. Modified kaolin (addition amount of 5.5kg / ton of urine) was added and mixed for 40min to obtain mixture I. S2. Perlite (addition amount of 2.0 kg / ton of urine) was added to mixture I and mixed for 4 hours. After standing for 20 hours, the clear liquid at the top was discarded. The remaining mixture was filtered through a plate and frame filter to obtain a filter cake. The filter cake was washed with acetic acid (addition amount of 15 L / ton of urine, pH value of 4.2) and then dried by compression and air blowing for 30 minutes to obtain kaolin adsorbent. S3. Add 300L of ammonia water to the kaolin adsorbent, adjust the pH to 11.5, mix for 2.5h, filter by plate and frame to obtain the first filter cake and the first filtrate. Add ammonia water to the first filter cake, adjust the pH to 11.5, mix for 2h, filter by plate and frame to obtain the second filter cake and the second filtrate. Combine the first filtrate and the second filtrate to obtain mixture II. S4. After adjusting the pH to 8.7 by adding acetic acid to mixture II, let it stand for 20 hours. Then add perlite (0.5 kg / ton of urine) and mix. After filtration through a plate and frame filter, collect the filtrate. Then, ultrafilter and concentrate the filtrate to 10% of the liquid volume to obtain the concentrated solution. S5. After adjusting the pH of the concentrate to 5.5 with acetic acid, add acetone (volume ratio of concentrate to acetone is 1:2) and mix for 25 minutes. Let stand for 10 hours, take the precipitate, filter it, dehydrate it with acetone, dry it, pass the precipitate through a 40-mesh sieve, and dry it at 0.10 MPa and 25℃ for 10 hours to obtain urinary gonadotropin. The preparation method of modified kaolin is as follows: A1. After dispersing chitosan evenly in a 2wt% acetic acid aqueous solution (the mass-volume ratio of chitosan to 2wt% acetic acid aqueous solution is 1g:50mL), kaolin is added and mixed for 3h to obtain premix solution I. A2. Liquid paraffin and Span 80 were added to premixed solution I and mixed at 40°C for 1 hour. Then, 25 wt% glutaraldehyde aqueous solution was added and reacted at 60°C for 1 hour. After washing with petroleum ether and anhydrous ethanol, the mixture was dried and pulverized to obtain kaolin composite chitosan with a particle size of 20 μm. The volume ratio of 2 wt% acetic acid aqueous solution, liquid paraffin, Span 80 and 25 wt% glutaraldehyde aqueous solution was 25 mL:100 mL:4 mL:3 mL. A3. After uniformly dispersing aminosilanes in an ethanol solution (composed of anhydrous ethanol and water in a mass ratio of 4:1), add kaolin composite chitosan and mix at 40°C for 40 minutes. After filtration and drying, modified kaolin is obtained. The mass-to-volume ratio of kaolin to ethanol solution is 1 g: 30 mL, the amino silane is 3-phenylaminopropyltrimethoxysilane, and the mass ratio of kaolin, chitosan and amino silane is 20:4:1. Results: The yield of urinary gonadotropin was 88.8%, and the purity was 96.0%.

[0028] Example 4 The only difference between this embodiment and Embodiment 2 is that in this embodiment, the mass ratio of kaolin, chitosan, and aminosilane is 50:10:3. Results: The yield of urinary gonadotropin was 91.6%, and the purity was 96.3%.

[0029] Example 5 The only difference between this embodiment and Embodiment 2 is that in this embodiment, the mass ratio of kaolin, chitosan, and aminosilane is 25:5:2. Results: The yield of urinary gonadotropin was 87.2%, and the purity was 95.5%.

[0030] Example 6 The only difference between this embodiment and Embodiment 2 is that the amino silane in this embodiment is N-(2-aminoethyl)-3-aminopropyltrimethoxysilane; Results: The yield of urinary gonadotropin was 88.1%, and the purity was 95.6%.

[0031] Example 7 The only difference between this embodiment and Embodiment 2 is that in this embodiment, the amino silane is 3-anilinopropyltrimethoxysilane and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane in a mass ratio of 2:1. Results: The yield of urinary gonadotropin was 92.3%, and the purity was 97.3%.

[0032] Example 8 The only difference between this embodiment and Embodiment 2 is that in this embodiment, the amino silane is 3-anilinepropyltrimethoxysilane and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane in a mass ratio of 2.5:1; Results: The yield of urinary gonadotropin was 95.0%, and the purity was 99.8%.

[0033] Example 9 The only difference between this embodiment and Embodiment 2 is that, in this embodiment, the amino silane is 3-anilinopropyltrimethoxysilane and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane in a mass ratio of 3:1; Results: The yield of urinary gonadotropin was 92.7%, and the purity was 97.7%.

[0034] Comparative Example 1 The only difference between this comparative example and Example 1 is that the modified kaolin is replaced with an equal amount of kaolin in this comparative example. Results: The yield of urinary gonadotropin was 73.2%, and the purity was 82.1%.

[0035] Comparative Example 2 The only difference between this comparative example and Example 1 is that the preparation method of the modified kaolin in this comparative example is as follows: 3-Aniaminopropyltrimethoxysilane was uniformly dispersed in an ethanol solution (composed of anhydrous ethanol and water in a mass ratio of 4:1), and then kaolin was added and mixed at 30°C for 60 min. After filtration and drying, modified kaolin was obtained. The mass-volume ratio of kaolin to ethanol solution was 1 g:30 mL, and the mass ratio of kaolin to 3-anilinepropyltrimethoxysilane was 20:1. Results: The yield of urinary gonadotropin was 81.4%, and the purity was 87.9%.

[0036] Comparative Example 3 The only difference between this comparative example and Example 1 is that the preparation method of the modified kaolin in this comparative example is as follows: A1. After dispersing chitosan evenly in a 2wt% acetic acid aqueous solution (the mass-volume ratio of chitosan to 2wt% acetic acid aqueous solution is 1g:50mL), kaolin is added and mixed for 2h to obtain premix solution I. A2. Liquid paraffin and Span 80 were added to premixed solution I and mixed at 30°C for 2 hours. Then, 25 wt% glutaraldehyde aqueous solution was added and reacted at 50°C for 2 hours. After washing with petroleum ether and anhydrous ethanol, the mixture was dried and pulverized to obtain kaolin composite chitosan with a particle size of 20 μm. The volume ratio of 2 wt% acetic acid aqueous solution, liquid paraffin, Span 80 and 25 wt% glutaraldehyde aqueous solution was 25 mL:100 mL:4 mL:3 mL, and the mass ratio of kaolin to chitosan was 5:1. Results: The yield of urinary gonadotropin was 81.8%, and the purity was 88.5%.

[0037] The above are merely preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. A method for extracting urinary gonadotropins from the urine of postmenopausal women, characterized in that, Includes the following steps: S1. After filtering the urine of postmenopausal women through a filter screen, the pH value is adjusted to 4-4.5, and modified kaolin is added and mixed to obtain mixture I; S2. After adding perlite I to mixed liquid I and mixing, let stand, discard the clear liquid on the top layer, filter through a plate and frame filter, wash and dry to obtain kaolin adsorbent. S3. Add desorption solution I to the kaolin adsorbent, adjust the pH value to 11~11.5 and mix. Filter the mixture through a plate and frame filter to obtain a first filter cake and a first filtrate. Add desorption solution II to the first filter cake, adjust the pH value to 11~11.5 and mix. Filter the mixture through a plate and frame filter to obtain a second filter cake and a second filtrate. Combine the first filtrate and the second filtrate to obtain mixture II. S4. After adjusting the pH of the mixture II to 8.2~8.7, let it stand, add perlite II and mix, let it stand, filter through a plate and frame filter to obtain the third filtrate, and then ultrafilter and concentrate the third filtrate to obtain the concentrated solution. S5. After adjusting the pH of the concentrate to 5-5.5, add acetone and mix. Let stand, filter to obtain precipitate. Dehydrate the precipitate with acetone, then sieve and dry to obtain urinary gonadotropin. The modified kaolin is obtained by first combining kaolin with chitosan, and then modifying it with amino silanes.

2. The method for extracting urinary gonadotropins from the urine of postmenopausal women according to claim 1, characterized in that, The mass ratio of kaolin, chitosan, and aminosilane is 100:20:5~8.

3. The method for extracting urinary gonadotropins from the urine of postmenopausal women according to claim 1, characterized in that, The modified kaolin is prepared as follows: A1. After dispersing chitosan evenly in solvent I, add kaolin and mix to obtain premixed solution I; A2. Liquid paraffin and Span 80 are added to premixed liquid I and mixed. Glutaraldehyde is added to react with the mixture. After washing, drying and pulverizing, kaolin composite chitosan is obtained. A3. After the amino silane is evenly dispersed in solvent II, kaolin and chitosan composite are added and mixed. After filtration and drying, modified kaolin is obtained.

4. The method for extracting urinary gonadotropins from the urine of postmenopausal women according to claim 3, characterized in that, In step A3, the mixing temperature is 30~40℃ and the mixing time is 40~60min.

5. The method for extracting urinary gonadotropins from the urine of postmenopausal women according to claim 1, characterized in that, The amino silanes include 3-anilinopropyltrimethoxysilane and N-(2-aminoethyl)-3-aminopropyltrimethoxysilane.

6. The method for extracting urinary gonadotropins from the urine of postmenopausal women according to claim 5, characterized in that, The mass ratio of 3-anilinopropyltrimethoxysilane to N-(2-aminoethyl)-3-aminopropyltrimethoxysilane is 2~3:

1.

7. The method for extracting urinary gonadotropins from the urine of postmenopausal women according to claim 1, characterized in that, In step S1, the mixing time is 20-40 minutes.

8. The method for extracting urinary gonadotropins from the urine of postmenopausal women according to claim 1, characterized in that, Both desorption solution I and desorption solution II are ammonia water.

9. The method for extracting urinary gonadotropins from the urine of postmenopausal women according to claim 1, characterized in that, In step S5, the mixing time is 15-25 minutes.

10. The method for extracting urinary gonadotropins from the urine of postmenopausal women according to claim 1, characterized in that, In step S5, the settling time is 8-10 hours.