An automated sampling device for cell culture supernatant microconstituent analysis

Abstract

This invention relates to the field of automated sampling technology and discloses an automated sampling device for the analysis of trace components in cell culture supernatant. The device includes a sampling worktable and a first robotic arm and a second robotic arm mounted thereon. The first robotic arm has a piston pump assembly at its end, and a sampling component is detachably mounted on its bottom via a threaded interface. The sampling component includes a sample sampling cylinder and a sampling head at the bottom. A suction port is provided on the side of the sampling head, and an insertion plug is slidably connected inside. An isolation balloon with a top opening is fixedly connected to the bottom end of the insertion plug. This invention uses the sampling cylinder directly as a storage container, eliminating residual loss on the inner wall during sample transfer. The isolation balloon effectively buffers the suction force, avoiding disturbance to the cell sedimentation layer, and adapts to differences in sedimentation layer thickness in each well, ensuring the purity and consistency of simultaneous multi-well sampling. This significantly improves the accuracy, reliability, and efficiency of automated micro-sample sampling.

Description

Technical Field

[0001] This invention relates to the field of automated sampling technology, and in particular to an automated sampling device for the analysis of trace components in cell culture supernatant. Background Technology

[0002] Cell culture supernatant sampling refers to the aseptic and non-destructive separation and collection of the liquid matrix covering the cell layer from a culture container, such as a multi-well plate or culture flask, during cell culture. Its core purpose is to obtain metabolites, cytokines, exosomes, drugs, or other soluble biomarkers secreted by cells into the extracellular environment for subsequent biochemical analysis or detection. This operation must precisely avoid the bottom cell layer and sediment while maintaining the stability of the culture system's microenvironment, thereby obtaining a representative sample that truly reflects the physiological state of the cells while minimizing interference with cell growth.

[0003] Because the amount of supernatant sample in each well is extremely limited, the residual effect of the inner wall of the sampling mechanism, such as the needle or pipette tip, will significantly reduce the sample volume, resulting in the actual amount of liquid transferred to the storage container for cryogenic transport being lower than the set value, which seriously affects the accuracy of subsequent quantitative analysis. Moreover, existing technologies generally use negative pressure aspiration. When the sampling needle is moved down to near the bottom of the well to pursue the maximum sample volume, the local suction will inevitably disturb the cell sediment layer below, causing cells or debris to be mixed into the sample. This not only contaminates the supernatant to be tested, but may also further interfere with the representativeness of the sample and the authenticity of the test results by aspirating non-target components. Summary of the Invention

[0004] The technical problem to be solved by this invention is that in the existing automated sampling technology for trace cell culture supernatant, sample loss and quantitative accuracy are caused by residue on the inner wall of the sampling needle, and the aspiration disturbance can easily cause cell layer mixing, thereby interfering with the authenticity of the sample and the detection results. To this end, we propose an automated sampling device for the analysis of trace components in cell culture supernatant.

[0005] To achieve the above objectives, this application adopts the following technical solution: an automated sampling device for the analysis of trace components in cell culture supernatant, comprising: a sampling worktable, a first robotic arm and a second robotic arm mounted above the sampling worktable, a piston pump assembly mounted at the end of the first robotic arm, the piston pump assembly including a first hydraulic rod fixedly connected to the output end of the first robotic arm, a fixed crossbar fixedly connected to the output end of the first hydraulic rod, a plurality of piston cylinders arranged at equal intervals at the bottom of the fixed crossbar, a threaded interface fixedly connected to the bottom end of the piston cylinders, and a sampling component mounted at the bottom of the threaded interface; The sampling assembly includes a threaded connector threaded into the threaded interface. A sample sampling cylinder is fixedly connected to the bottom of the threaded connector. A conical cylinder is fixedly connected to the bottom end of the sample sampling cylinder. A sampling head is fixedly connected to the bottom end of the conical cylinder. A liquid extraction port is provided on the side of the sampling head. An insertion plug is inserted into the inside of the sampling head. An isolation balloon is fixedly connected to the bottom end of the insertion plug.

[0006] Preferably, the insertion plug and the sampling head are slidably connected. During sampling, the top of the insertion plug is lower than the liquid extraction port. After sampling, the insertion plug is pushed inward to completely insert into the sampling head and block the liquid extraction port.

[0007] Preferably, the top of the isolation balloon is provided with an opening, and the sampling head is inserted into the interior of the isolation balloon through the opening.

[0008] Preferably, a sealing ring is fixedly connected to the inner side of the top opening of the isolation balloon. When the insertion plug is fully inserted into the sampling head, the sealing ring is tightly sealed against the outer wall of the conical cylinder.

[0009] Preferably, the top of the isolation balloon is arranged in a circular array with several sets of inserts, the cross-sectional shape of the inserts is set as a triangle with the tip facing outward, and the outer wall of the conical cylinder has an insertion port corresponding to each insert.

[0010] Preferably, the sampling assembly further includes a sealing cap, the size of which is consistent with the size of the threaded interface, and a limit block is fixedly connected to the outer wall of the sample sampling tube.

[0011] Preferably, a suction piston is slidably connected inside the piston cylinder, and a piston rod is fixedly connected to the top of the suction piston.

[0012] Preferably, a lifting crossbar is fixedly connected to the top end of the piston rod, the lifting crossbar is arranged parallel to the fixed crossbar, and second hydraulic rods are installed at both ends of the fixed crossbar, with the output end of the second hydraulic rods fixedly connected to the lifting crossbar.

[0013] Preferably, an unscrewing component, a capping and storage mechanism, a sampling tube storage mechanism, and a cell culture multi-well plate are sequentially arranged above the sampling workbench along the periphery of the first robotic arm, and a capping component is installed at the output end of the second robotic arm.

[0014] Preferably, the unscrewing assembly includes a mounting base fixedly connected to the top of the sampling workbench, and a plurality of screwing mechanisms are installed at equal intervals inside the mounting base. Two sets of limiting slots are symmetrically arranged on the top of the screwing mechanisms.

[0015] The technical effects and advantages of this invention are as follows: This invention uses the sample collection tube as the final storage container. The sample is directly aspirated and retained inside the sample collection tube. After sampling, only the sealing cap needs to be replaced to complete the sealing, eliminating intermediate transfer steps, maximizing the recovery rate of the supernatant, and ensuring the accuracy of subsequent quantitative analysis. The synergistic design of the isolation balloon and the insertion plug forms a multi-layered sealing protection after sampling, ensuring the stability of the sample during cryogenic transport. After sampling, pushing the insertion plug downwards seals the aspiration port. At the same time, the insertion block engages with the insertion port, the sealing ring is tightly attached to the outer wall of the conical tube, and the liquid seal formed by the residual liquid inside the balloon together constitutes three sealing barriers, effectively preventing external cryogenic nitrogen or dry ice gas from entering the sample, avoiding sample contamination or compositional changes, and allowing the device to be directly used for cryogenic storage and transport.

[0016] During sampling, the balloon is completely submerged below the liquid surface. The supernatant flows naturally into the balloon through its top opening. The suction force of the needle acts inside the balloon rather than directly on the bottom of the well, thus avoiding the mixing of cells or debris into the sample caused by negative pressure aspiration, ensuring the purity and representativeness of the sample. Simultaneously, this indirect aspiration method reduces sensitivity to needle position. Even if the balloon is close to the bottom of the well or partially embedded in the cell deposit layer, the collection port at its top can still stably collect the supernatant, addressing the issue of uneven cell deposit layer thickness in each well. Furthermore, the balloon can be partially embedded in the deposit layer, using the deposit layer as a natural reference surface, ensuring that the inlet is always located in the supernatant area. This avoids sampling port blockage or decreased collection efficiency due to differences in the deposit layer, thereby significantly improving the reliability and throughput of simultaneous multi-well sampling while maintaining sample purity. Attached Figure Description

[0017] The disclosure of this invention is illustrated with reference to the accompanying drawings. It should be understood that the drawings are for illustrative purposes only and are not intended to limit the scope of protection of this invention. In the drawings, the same reference numerals are used to refer to the same parts: Figure 1 This is a three-dimensional structural diagram of the entire invention; Figure 2 This is a three-dimensional structural schematic diagram of the piston pump assembly of the present invention; Figure 3 This is a schematic cross-sectional view of the piston cylinder and sample collection cylinder of the present invention. Figure 4 This is a cross-sectional structural diagram of the sampling state of the isolation balloon of the present invention; Figure 5 This is a schematic cross-sectional view of the sealed portion of the isolation balloon of the present invention. Figure 6 This is a three-dimensional structural diagram of the sample collection cylinder in a partially sealed state according to the present invention; Figure 7 This is a three-dimensional structural diagram of the unscrewing component part of the present invention.

[0018] Legend: 1. Sampling worktable; 2. First robotic arm; 3. Second robotic arm; 4. Piston pump assembly; 5. Sampling assembly; 6. Capping assembly; 7. Unscrewing assembly; 8. Capping storage mechanism; 9. Sampling cylinder storage mechanism; 10. Cell culture multi-well plate; 401. First hydraulic rod; 402. Fixed crossbar; 403. Piston cylinder; 404. Suction piston; 405. Piston rod; 406. Lifting crossbar; 4 07. Second hydraulic rod; 408. Threaded interface; 501. Sample sampling cylinder; 502. Threaded connector; 503. Conical cylinder; 504. Sampling head; 505. Liquid extraction port; 506. Insert plug; 507. Isolation balloon; 508. Sealing ring; 509. Insert block; 510. Insertion port; 511. Sealing cover; 512. Limiting block; 701. Mounting base; 702. Tightening mechanism; 703. Limiting slot. Detailed Implementation

[0019] It is readily understood that, based on the technical solution of this invention, those skilled in the art can propose various interchangeable structural methods and implementations without altering the essential spirit of the invention. Therefore, the following detailed embodiments and accompanying drawings are merely illustrative examples of the technical solution of this invention and should not be considered as the entirety of the invention or as limitations or restrictions on the technical solution of this invention.

[0020] Reference Figure 1 , Figure 2 and Figure 3 As shown, the present invention provides a technical solution: an automated sampling device for the analysis of trace components in cell culture supernatant, comprising: a sampling workbench 1, a first robotic arm 2 and a second robotic arm 3 mounted on the top of the sampling workbench 1, a piston pump assembly 4 mounted at the end of the first robotic arm 2, the piston pump assembly 4 including a first hydraulic rod 401 fixedly connected to the output end of the first robotic arm 2, a fixed crossbar 402 fixedly connected to the output end of the first hydraulic rod 401, a plurality of piston cylinders 403 arranged at equal intervals at the bottom of the fixed crossbar 402, a threaded interface 408 fixedly connected to the bottom end of the piston cylinders 403, a suction piston 404 slidably connected inside the piston cylinders 403, a piston rod 405 fixedly connected to the top of the suction piston 404, a lifting crossbar 406 fixedly connected to the top end of the piston rod 405, the lifting crossbar 406 and the fixed crossbar 402 being arranged parallel to each other, and a second hydraulic rod 407 mounted at both ends of the fixed crossbar 402, and the output end of the second hydraulic rod 407 being fixedly connected to the lifting crossbar 406; The lifting crossbar 406 is slidably connected to the first hydraulic rod 401. When the second hydraulic rod 407 drives the lifting crossbar 406 to rise or fall, it can simultaneously drive multiple sets of piston rods 405 to move the suction piston 404 upward, thus playing the role of negative pressure suction. Please see Figure 3 , Figure 4 and Figure 5 As shown, a sampling component 5 is installed at the bottom of the threaded interface 408. The sampling component 5 includes a threaded connector 502 that is threaded into the inside of the threaded interface 408. A sample sampling cylinder 501 is fixedly connected to the bottom of the threaded connector 502. A conical cylinder 503 is fixedly connected to the bottom end of the sample sampling cylinder 501. A sampling head 504 is fixedly connected to the bottom end of the conical cylinder 503. A liquid extraction port 505 is opened on the side of the sampling head 504. The connection between the threaded connector 502 and the threaded interface 408 connects and links the sample sampling cylinder 501 and the piston cylinder 403. The negative pressure generated by the upward movement of the suction piston 404 inside the piston cylinder 403 is transmitted to the sample sampling cylinder 501, allowing the sample liquid to be drawn into the sample sampling cylinder 501. After sampling, the sample liquid can be directly sealed and stored in the sample sampling cylinder 501 without transferring it to an additional storage tube. The bottom end of the sampling device directly serves as the sample storage tube. This design eliminates the intermediate step of discharging the sample into an external cryopreservation tube, preventing residual liquid from adhering to the inner wall of the sampling mechanism and causing waste of the supernatant, thereby maximizing the recovery rate of the supernatant and ensuring the accuracy of subsequent quantitative analysis.

[0021] Furthermore, the sample collection tube 501 is directly connected to the bottom of the piston tube 403 as the final storage container for the sample. During the sampling process, the piston tube 403 only serves as a power source to create a negative pressure environment without any direct contact with the liquid sample. This design completely eliminates the need for tedious cleaning of the sampling pipeline or sampling tip after sample transfer, avoiding the risk of cross-contamination caused by incomplete cleaning, and saving the consumption of cleaning fluid and waste liquid treatment, significantly improving the efficiency and convenience of automated processing.

[0022] Please see Figure 4 and Figure 5 As shown, an insertion plug 506 is inserted inside the sampling head 504. An isolation balloon 507 is fixedly connected to the bottom end of the insertion plug 506. An opening is provided at the top of the isolation balloon 507. The sampling head 504 is inserted into the interior of the isolation balloon 507 through the opening. Several sets of insertion blocks 509 are arranged in a ring array at the top of the isolation balloon 507. The cross-sectional shape of the insertion block 509 is a triangle with the tip facing outward. During sampling, the isolation balloon 507 is completely immersed below the liquid surface. The supernatant flows into the isolation balloon 507 through the top opening. The suction force of the sampling head 504 does not act directly on the cell culture multi-well plate 10, but rather on the inside of the isolation balloon 507. As a physical buffer chamber, the isolation balloon 507 confines the negative pressure of the suction force within the isolation balloon 507, effectively isolating the suction force from directly disturbing the bottom cell sediment layer. This prevents cells or debris from being rolled up and mixed into the sample due to strong local suction, thus ensuring the purity of the supernatant and the representativeness of the sample.

[0023] Furthermore, this indirect aspiration method reduces the sensitivity of the sampling head 504 position during the sampling process. Even if the isolation balloon 507 is close to the bottom of the cell culture well, or if part of the isolation balloon 507 has been submerged in the cell sediment layer, its top opening is still located in the supernatant layer for collection, which can maximize the recovery of precious samples without interfering with the cell layer below.

[0024] When sampling the cell culture supernatant in a multi-well plate, the thickness or confluence of the final cell deposit layer in each well can vary due to a combination of factors, including operational factors during cell plating (such as uneven addition of cell suspension, insufficient mixing, differences in initial sedimentation density caused by the plate not being placed horizontally), cell redistribution caused by shaking during plate transfer, environmental factors during culture (such as edge effects leading to different evaporation rates and temperature fluctuations between peripheral and central wells, thus affecting cell proliferation rate), and the sensitivity of cells to differences in the microenvironment.

[0025] Because the thickness or density of the cell deposit layer varies in different pores, extracting samples from each pore individually is inefficient. Simultaneous extraction from multiple pores in a row, with the sampling tip inserted synchronously, may result in over-insertion in pores with thicker deposit layers, clogging the pipette opening or drawing in cells, while insufficient insertion in pores with thinner deposit layers may prevent effective aspiration of the supernatant. In this solution, the bottom of the isolation balloon 507 can be embedded in the cell deposit layer during extraction, allowing the supernatant to still collect from the opening at its top into the isolation balloon. Inside the balloon 507, the sampling component 5 can maintain the same descent height for cell deposits of different thicknesses without the need for individual adjustments. The height of the isolation balloon 507 itself can adaptively buffer the effects of uneven deposit height. Multiple cell culture wells can be sampled simultaneously, avoiding the burial or blockage of the sampling port due to excessively high deposits and preventing a decrease in collection efficiency due to different deposit heights. Thus, while ensuring sample purity, the consistency and reliability of multi-well sampling are significantly improved.

[0026] The insertion plug 506 is slidably connected to the sampling head 504. During sampling, the top of the insertion plug 506 is lower than the liquid extraction port 505. After sampling, the insertion plug 506 is pushed inward to completely insert into the sampling head 504 and block the liquid extraction port 505. A sealing ring 508 is fixedly connected to the inner side of the top opening of the isolation balloon 507. When the insertion plug 506 is completely inserted into the sampling head 504, the sealing ring 508 is tightly attached to the outer wall of the conical cylinder 503 for sealing. The outer wall of the conical cylinder 503 has an insertion port 510 that corresponds one-to-one with the insertion block 509. After sampling, the insert plug 506 is inserted into the sampling head 504 to seal the liquid extraction port 505. At the same time, the insert block 509 is inserted into the insertion port 510, and the sealing ring 508 is tightly attached to the outer wall of the conical cylinder 503 to complete the second layer of sealing. The liquid remaining inside the isolation balloon 507 that was not completely sucked into the liquid extraction port 505 is blocked outside the liquid extraction port 505, which plays a role in liquid sealing. In the subsequent ultra-low temperature storage or transportation process, it can effectively prevent external ultra-low temperature nitrogen or dry ice gas from entering the conical cylinder 503 through the sampling head 504. Please see Figure 6 As shown, the sampling assembly 5 also includes a sealing cap 511. The size of the sealing cap 511 is consistent with the size of the threaded interface 408. A limit block 512 is fixedly connected to the outer wall of the sample sampling cylinder 501. After sampling, the sealing cap 511 is screwed on the threaded connector 502 to seal the top of the sample sampling cylinder 501.

[0027] Please see Figure 1 As shown, above the sampling workbench 1, along the periphery of the first robotic arm 2, are arranged a screw-on assembly 7, a capping storage mechanism 8, a sampling cylinder storage mechanism 9, and a cell culture multi-well plate 10. The screw-on assembly 7 includes a mounting base 701 fixedly connected to the top of the sampling workbench 1. Several screwing mechanisms 702 are installed at equal intervals inside the mounting base 701. Two sets of limiting slots 703 are symmetrically arranged on the top of the screwing mechanism 702. The screw-on assembly 7 is used to unscrew the sampling assembly 5 from the piston pump assembly 4. The capping storage mechanism 8 is used to store the sealing cap 511. The sampling cylinder storage mechanism 9 is used to store the sampling assembly 5 that has not yet been sampled.

[0028] Please see Figure 7 As shown, the output end of the second robotic arm 3 is equipped with a sealing assembly 6, which is used to remove the sealing cover 511 and complete the connection and sealing between the sealing cover 511 and the threaded joint 502.

[0029] Working principle: The cell culture multi-well plate 10 to be sampled slides and is locked to the side of the sampling worktable 1. The sample collection tube 501 is stored in the sample collection tube storage mechanism 9. The first robotic arm 2 first drives the piston pump assembly 4 to the sample collection tube storage mechanism 9, so that the threaded interface 408 is aligned with the threaded connector 502 at the top of each sample collection tube 501. At the same time, the rotating mechanism inside the sample collection tube storage mechanism 9 drives the sample collection tube 501 to rotate, completing the connection between the threaded connector 502 and the threaded interface 408. After the connection is completed, it moves together with the sample collection tube 501 to the cell culture multi-well plate 10 and inserts it into one row of cell culture wells. The end of the first robotic arm 2 is equipped with a vision recognition mechanism to ensure that the isolation balloon 507 is always below the liquid surface during sampling. When the isolation balloon 507 is fully open... After being submerged below the liquid surface, the supernatant flows into the interior of the isolation balloon 507 through the top opening of the isolation balloon 507. The insert block 509 is used to break the arch of the liquid and prevent it from being erected above the isolation balloon 507, so that the liquid can flow stably into the interior of the isolation balloon 507. At the same time, the top of the second hydraulic rod 407 pushes the lifting crossbar 406 to move upward. The lifting crossbar 406 pulls each set of piston rods 405 upward at the same time. The suction piston 404 slides upward inside the piston cylinder 403, forming a negative pressure at its bottom end, which is the sample sampling cylinder 501. Under the action of the negative pressure, the sampling head 504 draws the liquid inside the isolation balloon 507 into the conical cylinder 503 and the sample sampling cylinder 501 through the liquid extraction port 505. The amount of sample drawn is precisely controlled by the upward stroke of the suction piston 404. After quantitative sampling is completed, the first robotic arm 2 drives the piston pump assembly 4 and the sampling assembly 5 to the unscrewing assembly 7, so that the sampling tubes 501 of each group of samples are aligned and inserted above the screwing mechanism 702, and pressed down so that the insertion plug 506 is fully inserted into the sampling head 504, sealing the liquid extraction port 505. During the process of inserting the insertion plug 506 into the sampling head 504, its top can push the liquid originally adhering to the inner wall of the sampling head 504 upward into the conical cylinder 503. At the same time, the insertion block 509 is inserted into the insertion port 510, and the sealing ring 508 is inserted into the groove reserved on the outer wall of the conical cylinder 503 and fits tightly, completing the second layer of sealing. Meanwhile, the liquid remaining inside the isolation balloon 507 is wrapped and blocked outside the liquid extraction port 505, which plays the role of liquid seal, effectively preventing the low temperature liquid nitrogen from entering the sampling head 504 and affecting the composition of the sample liquid during subsequent transportation. Next, the screwing mechanism 702 drives the sample sampling cylinder 501 to rotate, causing the threaded connector 502 to disengage from the threaded interface 408. The second robotic arm 3 drives the capping assembly 6 to take the sealing cap 511 from the capping storage mechanism 8 and transfer it above the sample sampling cylinder 501. The screwing mechanism 702 drives the sample sampling cylinder 501 to rotate again, completing the connection and sealing between the threaded connector 502 and the sealing cap 511. After sealing, the sample sampling cylinder 501 and the liquid sample inside can be directly transferred to the freezer for storage or transport to the testing institution.

[0030] The technical scope of this invention is not limited to the content described above. Those skilled in the art can make various modifications and variations to the above embodiments without departing from the technical concept of this invention, and all such modifications and variations should fall within the protection scope of this invention.

Claims

1. An automated sampling device for microconstituent analysis of cell culture supernatant, characterized by, The device includes a sampling workbench, on which a first robotic arm and a second robotic arm are mounted. A piston pump assembly is mounted at the end of the first robotic arm. The piston pump assembly includes a first hydraulic rod fixedly connected to the output end of the first robotic arm. A fixed crossbar is fixedly connected to the output end of the first hydraulic rod. A plurality of piston cylinders are arranged at equal intervals at the bottom of the fixed crossbar. A threaded interface is fixedly connected to the bottom end of the piston cylinders. A sampling component is mounted at the bottom of the threaded interface. The sampling assembly includes a threaded connector threaded into the threaded interface. A sample sampling cylinder is fixedly connected to the bottom of the threaded connector. A conical cylinder is fixedly connected to the bottom end of the sample sampling cylinder. A sampling head is fixedly connected to the bottom end of the conical cylinder. A liquid extraction port is provided on the side of the sampling head. An insertion plug is inserted into the inside of the sampling head. An isolation balloon is fixedly connected to the bottom end of the insertion plug.

2. The automated sampling device for cell culture supernatant microconstituent analysis of claim 1, wherein: The insertion plug is slidably connected to the sampling head. During sampling, the top of the insertion plug is lower than the liquid extraction port. After sampling, the insertion plug is pushed inward to completely insert into the sampling head and block the liquid extraction port.

3. The automated sampling device for cell culture supernatant microconstituent analysis of claim 1, wherein: The isolation balloon has an opening at its top, and the sampling head is inserted into the interior of the isolation balloon through the opening.

4. The automated sampling device for cell culture supernatant microconstituent analysis of claim 3, wherein: A sealing ring is fixedly connected to the inside of the top opening of the isolation balloon. When the insertion plug is fully inserted into the sampling head, the sealing ring is tightly sealed against the outer wall of the conical cylinder.

5. The automated sampling device for cell culture supernatant microconstituent analysis of claim 1, wherein: The top of the isolation balloon is arranged in a circular array with several sets of inserts. The cross-sectional shape of the inserts is a triangle with the tip pointing outward. The outer wall of the conical cylinder has an insertion port that corresponds to each insert.

6. The automated sampling device for cell culture supernatant microconstituent analysis of claim 1, wherein: The sampling assembly also includes a sealing cap, the size of which is consistent with the size of the threaded interface, and a limit block is fixedly connected to the outer wall of the sample sampling tube.

7. The automated sampling device for trace component analysis of cell culture supernatant according to claim 1, characterized in that: A suction piston is slidably connected inside the piston cylinder, and a piston rod is fixedly connected to the top of the suction piston.

8. The automated sampling device for trace component analysis of cell culture supernatant according to claim 7, characterized in that: A lifting crossbar is fixedly connected to the top of the piston rod. The lifting crossbar and the fixed crossbar are arranged parallel to each other. Second hydraulic rods are installed at both ends of the fixed crossbar, and the output end of the second hydraulic rod is fixedly connected to the lifting crossbar.

9. The automated sampling device for trace component analysis of cell culture supernatant according to claim 1, characterized in that: Above the sampling workbench, along the periphery of the first robotic arm, are arranged a screw-on assembly, a capping and storage mechanism, a sampling tube storage mechanism, and a cell culture multi-well plate. The output end of the second robotic arm is equipped with a capping assembly.

10. The automated sampling device for trace component analysis of cell culture supernatant according to claim 9, characterized in that: The unscrewing assembly includes a mounting base fixedly connected to the top of the sampling workbench. Several screwing mechanisms are installed at equal intervals inside the mounting base, and two sets of limit latches are symmetrically arranged on the top of the screwing mechanisms.

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