Bacillus velezensis hp-bl2 and application thereof
By combining Bacillus vesiculus HP-BL2 with corn complex, processed soybean meal and wheat bran, the limited growth effect of Bacillus vesiculus in poultry and livestock farming in existing technologies has been solved, achieving improved intestinal health and enhanced immunity, and providing stable nutrient supply and antibacterial effect.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- QINGDAO HELP BIOSCI
- Filing Date
- 2026-03-11
- Publication Date
- 2026-06-09
AI Technical Summary
In existing technologies, Bacillus vesiculosus, as a microbial agent, has limited growth effects in poultry and livestock farming, especially in improving intestinal health and enhancing immunity.
By using Bacillus vesiculus HP-BL2, a stable feed formula is formed by combining microbial powder with corn complex, processed soybean meal and wheat bran, etc. Fermented Lactobacillus and chitosan are used to improve the intestinal environment, provide a variety of nutrients and antibacterial effects, and replace some antibiotics.
It significantly improves the intestinal health of poultry and livestock, enhances feed digestibility and immunity, reduces intestinal diseases, achieves a balanced supply and stable colonization of nutrients, and reduces dependence on antibiotics.
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Abstract
Description
Technical Field
[0001] This application relates to the field of microbial technology, and more specifically, to a Bacillus belesii HP-BL2 and its applications. Background Technology
[0002] Bacillus belyssus is a strain of Bacillus with broad-spectrum antibacterial, growth-promoting, and environmental adaptability capabilities. It is a Gram-positive bacterium that is aerobic or facultative anaerobic and can produce highly resistant spores. It has attracted attention due to its strong biocontrol capabilities, growth-promoting effects, and environmental adaptability. It is mainly used in green agriculture as a biopesticide or biofertilizer.
[0003] In the livestock and poultry farming sector, Bacillus vesiculosus, as a feed additive, regulates the intestinal flora, inhibits harmful bacteria such as Escherichia coli and Salmonella, improves feed digestibility, reduces the feed conversion ratio, enhances the immunity of livestock and poultry, and replaces some antibiotics.
[0004] In existing technologies, microbial agents made from Bacillus belyss as the main active ingredient through activation and fermentation processes have the advantages of strong stress resistance, stable activity, broad-spectrum function, and multiple effects from a single bacterium. However, their effects on the growth of poultry and livestock are limited. Summary of the Invention
[0005] In order to improve the limited effect of microbial agents on the growth of poultry and livestock, this application provides Bacillus vesiculosus HP-BL2 and its application.
[0006] Firstly, this application provides a Bacillus berberis HP-BL2 ( Bacillus velezensis HP-BL2), the strain is deposited at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M20242677, and deposit date November 29, 2024.
[0007] By adopting the above technical solutions, Bacillus vesiculosus HP-BL2 can achieve biological control in agricultural planting, inhibiting various plant pathogens, promoting growth and increasing yield, improving crop quality and output, improving soil, and optimizing the soil micro-ecological environment. In livestock and poultry farming, it can regulate the intestinal microecology, reduce intestinal diseases, improve feed digestibility, and enhance the body's immunity.
[0008] Secondly, this application provides the application of Bacillus vesiculosus HP-BL2 in feed.
[0009] Optionally, the feed, by weight, includes the following ingredients: 5-9 parts microbial powder, 25-30 parts corn compound, 10-15 parts wheat bran, and 15-20 parts processed soybean meal.
[0010] By employing the above-mentioned technical solutions, microbial inoculum powder improves animal intestinal health, inhibits pathogens, and enhances digestibility, ensuring high activity even after feed processing and storage. Corn complex provides easily digestible starch and carbohydrates, the foundation of energy for animal growth, while also supplementing dietary fiber and promoting intestinal motility. Wheat bran provides adequate crude fiber, promoting intestinal motility, maintaining normal digestive function, and serving as a good carrier for microbial inoculum powder and other ingredients, aiding in uniform mixing. Processed soybean meal is more easily digested and absorbed, exhibiting a synergistic effect with the digestive-promoting function of the microbial inoculum powder.
[0011] The core microbial powder in this application exhibits stable activity and no antagonistic effect with the raw materials. It can stably colonize the intestines of livestock and poultry, exerting antibacterial, digestive-promoting, and immune-enhancing effects, thus replacing some antibiotics. The combination of corn complex, treated soybean meal, and wheat bran achieves a balanced supply of energy, protein, and dietary fiber.
[0012] Optionally, the method for preparing the microbial powder includes the following steps: fermenting Bacillus belye HP-BL2 to obtain microbial powder.
[0013] By adopting the above technical solutions, the microbial powder prepared by Bacillus vesiculosus HP-BL2 can replace antibiotics, prevent intestinal diseases, improve intestinal health, increase feed conversion rate, enhance animal immunity, improve the breeding environment, and reduce emissions and protect the environment.
[0014] Optionally, the preparation method of the corn complex includes the following: Corn kernels, shrimp shell powder, and starch are ground into a mixture, which is then mixed with fermenting lactobacillus and fermented at 32-35℃ for 6-7 days at a pressure of 1.10-1.12×10⁻⁶. 5 Pa, yielding fermented material; Dry the fermented material in a well-ventilated place at room temperature for 2-3 hours, then add tangerine peel powder and egg white, stir for 2-3 hours to obtain a mixture, grind it into powder to obtain corn complex.
[0015] By employing the above-mentioned technical solution, grinding disrupts the structure, increases the surface area, and facilitates microbial attachment and enzymatic hydrolysis. Shrimp shell powder provides abundant minerals and high-quality animal protein and amino acids. During fermentation, the organic acids and enzymes produced by Lactobacillus fermentation partially degrade the chitin in the shrimp shell powder, generating prebiotic substances such as chitosan oligosaccharides. Simultaneously, the fermentation process releases proteins, minerals (calcium, phosphorus), and astaxanthin from the tightly bound matrix in the shrimp shell, improving bioavailability.
[0016] Lactobacillus fermentum produces large amounts of organic acids such as lactic acid and acetic acid, which have natural antibacterial and acidifying effects. Its secreted enzymes partially degrade anti-nutritional factors and complex carbohydrates in corn. It also converts some starch and protein into smaller, more easily digestible molecules. The micropressure environment inhibits aerobic bacteria, creating more favorable growth conditions for the facultative anaerobic Lactobacillus fermentum.
[0017] The fermented feed is air-dried to retain the activity of Lactobacillus fermentation, facilitating uniform mixing with subsequent powdered materials (dried tangerine peel powder) and preventing clumping. Tangerine peel powder aids digestion, and its volatile oils and flavonoids may regulate intestinal motility. It also provides antioxidants such as polyphenols, contributing to feed preservation and animal health. When combined with the fermented feed, it enhances the antioxidant and antibacterial bioactivity of the corn compound. Egg white provides a balanced amino acid profile of animal protein. During mixing and subsequent drying, it helps the components bind better, reducing dust in the finished product, creating an encapsulation effect, and protecting the active ingredients.
[0018] The resulting corn complex has a uniform particle size, improving its solubility, dispersibility, and palatability, making it easier to store, transport, and process. It maintains intestinal microecological balance, reducing reliance on antibiotics. Overall, it improves the digestibility and absorption of basic feed, enhances the animal's antioxidant and stress resistance capabilities, and increases farming efficiency.
[0019] Optionally, the mass ratio of corn kernels, shrimp shell powder, and starch is 1:0.41-0.52:0.63-0.71.
[0020] By adopting the above technical solution, corn kernels, as the core raw material base, provide natural nutrition and structural framework, offering basic components such as corn polysaccharides, dietary fiber, plant protein, and natural minerals, which are the core sources of the final product's nutrition and function. Shrimp shell powder provides chitin / protein complexes, a key source of the fermentation target products—chitosan oligosaccharides, free amino acids, and peptides; it also provides abundant calcium, phosphorus, and astaxanthin, which are converted or released during fermentation, improving bioavailability.
[0021] Starch provides a high-purity, easily degradable, high-quality carbon source for Lactobacillus fermentation, rapidly initiating strain proliferation and solving the problems of difficult carbon source degradation and slow fermentation start-up in corn kernels. The hydrophilic groups of starch can adsorb a large amount of water, maintaining a suitable moisture content in the mixture and providing a sufficient water environment for bacterial metabolism. The synergistic effect of the three components constructs a gradient carbon source system, ensuring nutrient supply throughout fermentation, achieving homogenization of the solid substrate, improving fermentation reaction efficiency, pre-embedding an acid-base buffer system, stabilizing the fermentation microenvironment in advance, regulating substrate physical properties, and adapting to fermentation process operations.
[0022] Optionally, the method for preparing the processed soybean meal includes the following: Soybean meal is steamed for 10-15 minutes, then dispersed in water, acetic acid and chitosan are added, stirred for 1-2 hours, freeze-dried, and ground to obtain a mixture; Sodium alginate, water, microcrystalline cellulose, and sodium polyacrylate are stirred evenly to obtain a mixture. The mixture is then added to the mixture and spray-dried to obtain processed soybean meal.
[0023] By employing the above technical solution, raw soybean meal contains trypsin inhibitors and lectins, which can be denatured and inactivated by cooking, improving the digestibility of the protein, killing potential pathogenic microorganisms in the soybean meal, and loosening the protein structure to expose more reaction sites, which is beneficial for subsequent binding with substances such as chitosan. Acetic acid provides an acidic environment under which chitosan can dissolve into positively charged chain molecules. At the same time, the acidic environment also helps to further destroy residual anti-nutritional factors.
[0024] The amino groups on the chitosan molecular chain are electrostatically attracted to the negatively charged groups of soybean meal proteins, and can also bind through hydrogen bonds, thus coating the surface of soybean meal particles. Chitosan itself has natural antibacterial properties, the ability to adsorb mycotoxins, and can regulate immunity. Freeze-drying can prevent the thermal denaturation of chitosan and proteins to the greatest extent, retain their biological activity, and form a porous and fluffy solid structure.
[0025] Sodium alginate is the core film-forming material, while microcrystalline cellulose increases the viscosity of the mixture, prevents solid particles from settling, and enhances the mechanical strength of the particles after spray drying. Sodium polyacrylate helps to rapidly solidify and shape the particles during the drying process, and spray drying enables microencapsulation, resulting in a functionally stable final product.
[0026] The wall material (such as sodium alginate) quickly forms a dense or semi-permeable protective film on the surface of soybean meal particles, encapsulating active ingredients such as chitosan and protein. This results in a powder with good flowability, uniform particle size, and resistance to moisture absorption and clumping, facilitating feed processing and storage.
[0027] Optionally, the mass ratio of soybean meal, chitosan, and microcrystalline cellulose is 1:0.35-0.43:0.12-0.18.
[0028] By adopting the above technical solution and further limiting the mass ratio of soybean meal, chitosan, and microcrystalline cellulose within a certain range, the resulting treated soybean meal exhibits superior overall performance. Soybean meal suffers from poor water solubility and aqueous dispersion due to the exposed hydrophobic groups of soybean protein and the high crystallinity of its polysaccharides. When the three components are mixed, the strong hydrophilic hydroxyl / amino groups of chitosan and the hydrophilic hydroxyl groups of microcrystalline cellulose adhere together to the surface of the soybean meal particles, forming a hydrophilic film. Simultaneously, the chitosan molecular chains can penetrate into the spaces between soybean meal protein molecules, disrupting the hydrophobic aggregation structure of the protein and exposing the hydrophilic groups of the soybean meal itself. The interweaving of the hydrophilic groups of the three components forms a hydrophilic network, significantly improving the water retention, water solubility, and aqueous dispersion of the core material. This lays the foundation for the rapid dissolution of the product after subsequent spray drying and prevents precipitation and aggregation in the aqueous system.
[0029] Chitosan molecular chains form hydrogen bonds and electrostatic interactions with the amino / carboxyl groups of soybean meal protein, cross-linking and fixing the modified soybean meal protein to prevent secondary denaturation of protein molecules during subsequent processing, thus preserving its digestibility and nutritional value. The rigid structure of microcrystalline cellulose forms a physical protective layer on the surface of the core material particles, isolating some of the influence of the external environment, while reducing excessive entanglement of chitosan molecular chains and preserving the bioactive sites of chitosan. The composite structure formed by these three components prevents structural collapse of the core material in the short-term high-temperature environment of spray drying, ensuring that the porous structure of the core material is not damaged, thus ensuring the applicability of the final product. This constructs a protein-polysaccharide-dietary fiber ternary nutritional system, achieving nutritional complementarity and functional value-added benefits.
[0030] Preferably, the feed is feed for raising poultry and livestock, such as chickens, ducks, and pigs.
[0031] In summary, this application has the following beneficial effects: 1. The Bacillus vesiculosus HP-BL2 of this application can regulate the intestinal microecology, reduce intestinal diseases, improve feed digestibility, and enhance the body's immunity in the field of livestock and poultry breeding.
[0032] 2. The feed microbial powder of this application has stable activity and no antagonistic effect with raw materials. It can stably colonize in the intestines of livestock and poultry, exert antibacterial, digestive, and immune-enhancing effects, and replace some antibiotics.
[0033] 3. The microbial powder prepared by Bacillus vesiculosus HP-BL2 of this application can prevent intestinal diseases, improve intestinal health, increase feed conversion rate, enhance animal immunity, and improve the breeding environment. Detailed Implementation
[0034] Example 1 Isolation and screening of Bacillus belye: Soil samples were isolated from the root zone of a cucumber plant at a cucumber planting base in Laoshan, Qingdao. Bacillus belyssus HP-BL2 was isolated from the soil and subjected to morphological analysis.
[0035] The strain was activated on plates and incubated in an incubator for 72 hours. The physical morphology of the colonies was observed and recorded. The culture morphology of Bacillus belye HP-BL2: On LB medium, it presented as opaque colonies with a rough surface, Gram staining, and were round or nearly round.
[0036] Disperse 10g of soil that has passed through a 30-mesh sieve into 100mL of sterile deionized water, shake at 160rpm for 35min, let stand for 8min, take the supernatant, add 9mL of sterile deionized water to 1mL to prepare 10mL of solution. -1 Soil microbial suspension, serially diluted to 10... -2 10 -3 10 -4 10 -5 10 -6 Soil suspension.
[0037] The above 10 -1 -10 -7 The soil gradient dilution was treated at 85°C for 15 minutes, then cooled to room temperature, and 10 samples were taken. -3 -10 -7 50 μL of the dilution solution was spread onto LB agar plates (NaCl 10.0 g / L, tryptone 10.0 g / L, yeast extract 5.0 g / L, pH adjusted to 7.0 with NaOH solution, agar 20 g / L). The spread LB agar plates were incubated at 37℃ for 1 day. Single colonies were selected to obtain the primary screening strain. The primary screening strain was inoculated into LB agar and incubated at 37℃ for 2 days for isolation and purification to obtain the selected strain. The strain was stored at -40℃.
[0038] The purified strain was named *Bacillus belyesense* HP-BL2, deposited at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M20242677, deposited on November 29, 2024, and classified as *Bacillus belyesense* HP-BL2. Bacillus velezensis HP-BL2).
[0039] Application Example 1 The feed, by weight, includes the following ingredients: 7 kg of microbial inoculum powder, 28 kg of corn compound, 12 kg of wheat bran, and 18 kg of processed soybean meal.
[0040] The preparation method of microbial inoculum powder is as follows: After culturing Bacillus belye HP-BL2 on NA medium for 48 h, a loopful of a single colony was picked and inoculated into NB medium, incubated at 30 °C and 180 rpm. -1 After shaking culture for 24 h, the seed culture was obtained. The seed culture was then mixed with NYBD culture medium at a volume ratio of 1:100 and incubated at 30 °C and 180 r·min. -1 Fermentation broth was obtained by shaking culture under the specified conditions for 48 hours. The spore concentration was 1×10⁻⁶ using the plate spreading method. 8 CFU·mL -1 .
[0041] NA medium: 5g beef extract, 10g peptone, 5g NaCl, 20g agar powder, 20g starch, 1000mL distilled water, pH 7.0.
[0042] NB culture medium: 5g beef extract, 10g peptone, 5g NaCl, 20g molasses, 1000mL distilled water, pH 7.0.
[0043] NYBD culture medium: 8g beef extract, 5g yeast extract, 10g glucose, 2g sodium citrate, 1000mL distilled water, pH 7.0.
[0044] The fermentation broth was added to diatomaceous earth, with a fermentation broth:carrier (v / w) ratio of 1:1, and fermented (30℃, 70% ambient humidity, fermentation for 48 hours). After drying and pulverizing, Bacillus belyssus HP-BL2 bacterial powder was obtained.
[0045] The preparation method of the corn complex includes the following: 30 kg of corn kernels, shrimp shell powder, and starch were ground into a mixture, which was then mixed with fermenting lactobacillus and fermented at 35°C for 7 days at a pressure of 1.12 × 10⁻⁶. 5 Pa, yielding fermented material; The fermented material was dried in a ventilated place at room temperature for 3 hours, and then 8 kg of dried tangerine peel powder and 15 kg of egg white were added and stirred for 2.3 hours to obtain a mixture. The mixture was then ground into powder to obtain the corn complex.
[0046] The mass ratio of corn kernels, shrimp shell powder, and starch is 1:0.41:0.71.
[0047] The methods for preparing soybean meal include the following: 20 kg of soybean meal was steamed for 13 minutes (at 100°C), then dispersed in 50 L of water, 6 L of 5% acetic acid and chitosan were added, stirred for 2 hours, freeze-dried (freezing temperature -40°C, vacuum degree 10 Pa, drying time 12 hours), and ground to obtain a mixture. 10 kg of sodium alginate, 60 L of water, microcrystalline cellulose, and 3 kg of sodium polyacrylate were stirred evenly to obtain a mixture. The mixture was then added to the mixture and spray-dried (inlet air temperature 160℃, outlet air temperature 70℃, feed rate 10 mL / min, atomization pressure 0.2 MPa) to obtain processed soybean meal.
[0048] The mass ratio of soybean meal, chitosan, and microcrystalline cellulose was 1:0.35:0.12.
[0049] Application Example 2 The feed differs from Application Example 1 in that, by weight, it includes the following ingredients: 9 kg of microbial inoculum powder, 25 kg of corn compound, 10 kg of wheat bran, and 15 kg of processed soybean meal.
[0050] Application Example 3 The feed differs from Application Example 1 in that, by weight, it includes the following ingredients: 5 kg of microbial inoculum powder, 30 kg of corn compound, 15 kg of wheat bran, and 20 kg of processed soybean meal.
[0051] Application Example 4 The difference between the feed and application example 1 lies in the preparation method of the corn compound, which includes the following: grinding 30 kg of corn kernels and starch to obtain a mixture, adding lactobacillus fermentation, and fermenting at 35°C for 7 days at a pressure of 1.12 × 10⁻⁶. 5 Pa, yielding fermented material.
[0052] Application Example 5 The feed differs from Application Example 1 in that 30 kg of corn kernels and shrimp shell powder are ground into a mixture, fermented with lactobacillus, and fermented at 35°C for 7 days at a pressure of 1.12 × 10⁻⁶. 5 Pa, yielding fermented material.
[0053] Application Example 6 The feed differs from that in Application Example 1 in that the mass ratio of corn kernels, shrimp shell powder, and starch is 1:0.52:0.63.
[0054] Application Example 7 The feed differs from that in Application Example 1 in that the mass ratio of corn kernels, shrimp shell powder, and starch is 1:0.12:0.98.
[0055] Application Example 8 The difference between the feed and application example 1 is that the fermented feed was dried in a ventilated place at room temperature for 3 hours, then 15 kg of egg white was added and stirred for 2.3 hours to obtain a mixture, which was then ground into powder to obtain a corn complex.
[0056] Application Example 9 The difference between this feed and application example 1 is that the fermented feed was dried in a ventilated place at room temperature for 3 hours, then 8 kg of dried tangerine peel powder was added and stirred for 2.3 hours to obtain a mixture, which was then ground into powder to obtain a corn complex.
[0057] Application Example 10 The difference between this feed and application example 1 lies in the method for preparing soybean meal, which includes the following: 20 kg of soybean meal was steamed for 13 minutes, then dispersed in 50 L of water, stirred for 2 hours, freeze-dried, and ground to obtain a mixture.
[0058] Application Example 11 The difference between this feed and application example 1 lies in the method for preparing soybean meal, which includes the following: 10 kg of sodium alginate, 60 L of water, and 3 kg of sodium polyacrylate were stirred evenly to obtain a mixture. The mixture was then added to the solution and spray-dried to obtain processed soybean meal.
[0059] Application Example 12 The difference between this feed and application example 1 is that the mass ratio of soybean meal, chitosan, and microcrystalline cellulose is 1:0.43:0.18.
[0060] Application Example 13 The difference between this feed and application example 1 is that the mass ratio of soybean meal, chitosan, and microcrystalline cellulose is 1:0.13:0.42.
[0061] Application Comparative Example 1 The feed differs from that in Application Example 1 in that it does not contain corn compound.
[0062] Application Comparative Example 2 The difference between this feed and application example 1 is that no processed soybean meal is added.
[0063] Animal experiments Broiler experiment: Broilers at 11, 28, and 31 days of age were weighed on an empty stomach. Feed intake was recorded from 11 to 28 days and from 28 to 31 days of age. Average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (F / G) were calculated. Specifically, Average Daily Gain (ADG) = (body weight at the end of the experiment - body weight at the beginning of the experiment) / (number of days in the experiment × number of chickens per group); Average Daily Feed Intake (ADFI) = feed intake during the experiment / (number of days in the experiment × number of chickens per group); Feed conversion ratio (F / G) = Average daily feed intake / Average daily gain. The test results are shown in Table 1.
[0064] Table 1. Test Results
[0065] Pig experiment: Weaned pigs were fed for 7 days, 14 days, and 21 days, respectively. Feed intake was recorded from 7 to 14 days and from 14 to 21 days. Average daily gain (ADG), average daily feed intake (ADFI), and feed conversion ratio (F / G) were calculated. The results are shown in Table 2.
[0066] Table 2 Test Results
[0067] The feeds prepared in Application Examples 1-3, 6, and 12 of this application were tested on broilers and pigs. As shown in Tables 1 and 2, in Application Example 1, for broilers, the average daily weight gain was 20.1 ± 2.34 g / bird, the average daily feed intake was 26.35 ± 0.35 g, and the feed conversion ratio was 1.32; for pigs, the average daily weight gain was 334.16 ± 12.6 g / bird, the average daily feed intake was 672.35 ± 14.36 g, and the feed conversion ratio was 2.01. This indicates that the feed prepared in this application has a good promoting effect on the growth of poultry and livestock. The microbial powder has stable activity and no antagonistic effect with the raw materials. It can stably colonize in the intestines of livestock and poultry, exerting antibacterial, digestive-promoting, and immune-enhancing effects. The combination of corn complex, treated soybean meal, and wheat bran achieves a balanced supply of energy, protein, and dietary fiber.
[0068] In Application Examples 4-5, shrimp shell powder and starch were not added to the preparation method of the corn complex. In Application Examples 6-7, the mass ratio of corn kernels, shrimp shell powder, and starch was changed. As shown in Tables 1 and 2, the test results of Application Examples 4-5 on the average daily weight gain and feed conversion ratio of broilers and pigs were significantly worse than those of Application Examples 1-3 and Application Example 6. The test results of Application Example 7 on the average daily weight gain and feed conversion ratio of broilers and pigs were better than those of Application Examples 4-5, but worse than those of Application Examples 1-3 and Application Example 6, indicating that shrimp shell powder provides abundant calcium and phosphorus. Astaxanthin and other components may be converted or released during fermentation, improving bioavailability; starch provides high-purity, easily degradable, high-quality carbon source for fermenting lactobacillus, rapidly initiating strain proliferation and solving the problems of difficult carbon source degradation and slow fermentation start-up in corn kernels; the three components work synergistically to construct a gradient carbon source system, ensuring nutrient supply throughout fermentation, achieving homogenization of the solid matrix, improving fermentation reaction efficiency, and pre-embedding an acid-base buffer system to stabilize the fermentation microenvironment in advance, regulate the physical properties of the matrix, and adapt to fermentation process operations.
[0069] In Application Examples 8-9, the corn complex preparation methods did not include the addition of dried tangerine peel powder or egg white. As shown in Tables 1 and 2, the test results of Application Examples 8-9 on the average daily weight gain and feed conversion ratio of broilers and pigs were significantly worse than those of Application Examples 1-3. This indicates that dried tangerine peel powder aids digestion, and its volatile oils and flavonoids may have the function of regulating intestinal peristalsis, providing antioxidant components such as polyphenols, which helps maintain feed quality and animal health. When compounded with fermented feed, it enhances the antioxidant, antibacterial and other biological activities of the corn complex. Egg white provides animal protein with a balanced amino acid profile. During stirring and subsequent drying, it helps the components to bind better, reduces dust in the finished product, forms an encapsulation effect, and protects the active ingredients.
[0070] In Application Examples 10-11, chitosan and microcrystalline cellulose were not added to the soybean meal preparation methods, respectively. In Application Examples 12-13, the mass ratio of soybean meal, chitosan, and microcrystalline cellulose was changed. Tables 1 and 2 show that Application Examples 10-11 showed significantly worse results in terms of average daily weight gain and feed conversion ratio for broilers and pigs compared to Application Examples 1-3 and 12. Application Example 13 showed better results in terms of average daily weight gain and feed conversion ratio for broilers and pigs than Application Examples 10-11, but worse than Application Examples 1-3 and 12. This indicates that the amino groups on the chitosan molecular chain are related to the soybean meal. The negatively charged groups of soybean meal protein are electrostatically attracted and can also bind through hydrogen bonds, thus coating the surface of soybean meal particles. Chitosan itself has natural antibacterial properties, the ability to adsorb mycotoxins, and can regulate immunity. Microcrystalline cellulose increases the viscosity of the mixture, prevents solid particles from settling, and enhances the mechanical strength of the particles after spray drying. The wall material (such as sodium alginate) quickly forms a dense or semi-permeable protective film on the surface of soybean meal particles, embedding active ingredients such as chitosan and protein inside, resulting in a powder with good flowability, uniform particle size, and is not prone to moisture absorption and clumping, which is convenient for feed processing and storage.
[0071] In Comparative Examples 1-2, the feeds contained neither corn complex nor treated soybean meal. Tables 1 and 2 show that Comparative Examples 1-2 significantly outperformed Comparative Examples 1-2 in terms of average daily weight gain and feed conversion ratio for broilers and pigs. This indicates that the corn complex provides easily digestible starch and carbohydrates, which are the foundation of energy for animal growth, while also supplementing dietary fiber and promoting intestinal peristalsis. The treated soybean meal is more easily digested and absorbed, and has a synergistic effect with the digestive-promoting function of the microbial powder, jointly improving the growth of poultry and livestock.
[0072] This specific embodiment is merely an explanation of this application and is not intended to limit it. After reading this specification, those skilled in the art can make modifications to this embodiment without contributing any inventive step, but such modifications are protected by patent law as long as they fall within the scope of the claims of this application.
Claims
1. A type of Bacillus belyssus HP-BL2 ( Bacillus velezensis HP-BL2), characterized in that, The strain is deposited at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M20242677 and deposit date of November 29, 2024.
2. The application of Bacillus vesiculosus HP-BL2 in feed according to claim 1.
3. The application according to claim 2, characterized in that, The feed, by weight, comprises the following ingredients: 5-9 parts microbial powder, 25-30 parts corn compound, 10-15 parts wheat bran, and 15-20 parts processed soybean meal.
4. The application according to claim 3, characterized in that, The method for preparing the microbial powder includes the following steps: fermenting Bacillus belye HP-BL2 to obtain microbial powder.
5. The application according to claim 3, characterized in that, The preparation method of the corn complex includes the following: Corn kernels, shrimp shell powder, and starch are ground into a mixture, which is then mixed with fermenting lactobacillus and fermented at 32-35℃ for 6-7 days at a pressure of 1.10-1.12×10⁻⁶. 5 Pa, yielding fermented material; Dry the fermented material in a well-ventilated place at room temperature for 2-3 hours, then add tangerine peel powder and egg white, stir for 2-3 hours to obtain a mixture, grind it into powder to obtain corn complex.
6. The application according to claim 5, characterized in that, The mass ratio of corn kernels, shrimp shell powder, and starch is 1:0.41-0.52:0.63-0.
71.
7. The application according to claim 3, characterized in that, The method for preparing processed soybean meal includes the following: Soybean meal is steamed for 10-15 minutes, then dispersed in water, acetic acid and chitosan are added, stirred for 1-2 hours, freeze-dried, and ground to obtain a mixture; Sodium alginate, water, microcrystalline cellulose, and sodium polyacrylate are stirred evenly to obtain a mixture. The mixture is then added to the mixture and spray-dried to obtain processed soybean meal.
8. The application according to claim 7, characterized in that, The mass ratio of soybean meal, chitosan, and microcrystalline cellulose is 1:0.35-0.43:0.12-0.
18.
9. The application according to claim 2, characterized in that, The feed is for raising poultry and livestock, namely chickens, ducks, and pigs.