Beta-gelling enzyme, method for preparing beta-gelling enzyme, application and method for preparing neojosamicrose

By screening marine microbial strains Marinimicrobium sp. and Escherichia coli expression systems, and optimizing induction culture conditions, the problems of long reaction time, harsh conditions, and complex products of existing β-agarases were solved, achieving the efficient generation of a single new agarbiose.

CN122168571APending Publication Date: 2026-06-09AQUABRAIN BIOTECH XIAMEN CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
AQUABRAIN BIOTECH XIAMEN CO LTD
Filing Date
2026-05-09
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing β-agarases have long reaction times, demanding conditions, poor enzymatic hydrolysis ability, and complex products, making it difficult to efficiently generate a single new agarbiose.

Method used

By screening marine microbial strain Marinimicrobium sp., a highly efficient β-agarase was obtained and an expression strain was constructed. Using host cell expression systems such as Escherichia coli, the induction culture conditions were optimized to achieve efficient and specific degradation of agar oligosaccharides, generating a single product, neoagarbiose.

Benefits of technology

Efficient and specific degradation of agar oligosaccharides was achieved under mild conditions, generating a single product, neo-agarbiose, which has high enzyme activity and stability, making it suitable for large-scale industrial production.

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Abstract

The application belongs to the technical field of biotechnology, and discloses a beta-agarase, a method for preparing the beta-agarase, application and a method for preparing neoaarabinose. The beta-agarase provided by the application specifically includes an amino acid fragment as shown in SEQ ID NO: 1 or a variant fragment with at least 90% sequence identity with the amino acid fragment as shown in SEQ ID NO: 1, which can efficiently and specifically realize degradation of agar oligosaccharide under mild conditions and the degradation product is a single product neoaarabinose, and has the advantages of high enzyme activity, mild reaction, high stability and uniform enzymatic product, and has excellent application prospects in realizing large-scale industrial production of neoaarabinose.
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