Nucleic acid rapid extraction kit for high oil crop transgene detection and application

This rapid nucleic acid extraction kit, composed of lysis buffer, precipitation buffer, and neutralization buffer, solves the problem of low nucleic acid extraction efficiency in high-oil crops, achieving rapid and effective nucleic acid extraction and accurate PCR reaction, and is suitable for transgenic detection in high-oil crops.

CN122168724APending Publication Date: 2026-06-09大连海关技术中心
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
大连海关技术中心
Filing Date
2026-05-09
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

In existing genetically modified organism (GMO) detection methods, nucleic acid extraction efficiency is low for high-oil crops, and oil and protein inhibitors are difficult to remove, leading to PCR reaction failure or false negative results. Furthermore, existing rapid release agents cannot effectively process high-oil samples.

Method used

This rapid nucleic acid extraction kit, consisting of lysis buffer, precipitation buffer, and neutralization buffer, includes lysis buffers A and A-Plus, precipitation buffer, and neutralization buffer. Through alkaline lysis, neutralization, and precipitation purification steps, it removes oils and proteins within 15 minutes and is suitable for the detection of transgenic crops with high oil content.

Benefits of technology

It enables rapid extraction of nucleic acids from high-oil crops, removing oil and protein inhibitors within 15 minutes to ensure the accuracy of subsequent PCR reactions. It is suitable for TaqMan qPCR and SYBR Green qPCR detection, and is cost-effective and easy to operate.

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Abstract

The application belongs to the technical field of molecular biology, and particularly relates to a nucleic acid rapid extraction kit for transgenic detection of high-oil crops and application, and is particularly suitable for fluorescent quantitative PCR detection of transgenic components of high-oil crops. The kit comprises a lysis solution, a precipitation solution, a neutralization solution and an elution solution. The application can be completed within 15 minutes, effectively removes PCR inhibitors such as oil and protein, is specifically used for high-oil crops, and is a low-cost, safe and non-toxic nucleic acid rapid extraction method, and meets the requirement of on-site rapid detection of transgenic crops.
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