InDel molecular marker closely linked with citrus grandis peel color and application of InDel molecular marker

By developing an InDel molecular marker closely linked to the color of pomelo peel, and using primer pair F/R for PCR amplification and electrophoretic analysis, the problem of early screening of red-skinned pomelo peel color in the seedling stage was solved, thus shortening the breeding cycle and improving screening efficiency.

CN122168797AActive Publication Date: 2026-06-09HUAZHONG AGRI UNIV +2
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Patent Information

Application Number
CN202610647063.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-05-12
Publication Date
2026-06-09
Estimated Expiration
2046-05-12

AI Technical Summary

Technical Problem

Existing technologies cannot achieve early screening of the peel color of red-skinned pomelos during the seedling stage, resulting in a long breeding cycle and limiting the efficient screening of red-skinned pomelos with high ornamental and economic value.

Method used

A novel InDel molecular marker closely linked to the color of pomelo peel was developed. PCR amplification was performed using primer pair F/R, and the band properties of the amplified products were analyzed by electrophoresis to achieve rapid identification of peel color during the seedling stage.

Benefits of technology

It significantly shortens the breeding cycle, improves the screening efficiency of red-skinned pomelo germplasm, breaks the dependence on fruit ripening period, and achieves early and efficient screening.

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Abstract

The invention discloses an InDel molecular marker closely linked with the color of citrus grandis peel and application of the InDel molecular marker. The nucleotide sequence of the InDel molecular marker is as shown in SEQ ID No.1. According to the present invention, the kit for identifying the citrus grandis peel color is obtained, and the problems of long period, low efficiency and the like due to the fact that the traditional method depends on field fruit phenotype observation can be overcome by using the kit to identify the citrus grandis red peel and the citrus grandis clausena lansium. By using a seedling stage molecular marker diagnosis technology, early screening of pericarp color traits can be realized in a grapefruit seedling stage, the dependence on a fruit ripening season is broken through, and a breeding period is remarkably shortened. The kit is simple to operate and low in cost, can be used for rapidly screening and identifying target single plants in large-scale filial generation groups, and greatly accelerates the breeding process of grapefruit germplasm with excellent peel color.
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