Intelligent biochip detection all-in-one machine control system

Abstract

This invention discloses an integrated control system for an intelligent biochip detection system, relating to the field of biochip detection technology. It includes: an information matching module for collecting information on the type of chip to be detected and the detection requirements, and matching the corresponding target workstation and detection process parameters; a transport and dispensing module for performing chip gripping, transporting, and precise positioning between the chip chamber and the target workstation, and performing sample needle assembly, reagent aspiration, chip reaction well dispensing, and post-use sample needle removal and recovery; and a reaction waste liquid module for outputting the temperature and mixing conditions required for the chip reaction according to the matched detection process parameters, and performing the extraction and directional delivery of waste liquid from the chip reaction wells. This invention achieves integrated adaptive control for multi-type chip detection through chip information recognition, process matching, and unified coordination of the entire process execution mechanism, eliminating the need for manual parameter setting and providing a single system compatible with detection processes for different chips.

Description

Technical Field

[0001] This invention relates to the field of biochip detection technology, specifically to a smart biochip detection integrated machine control system. Background Technology

[0002] The intelligent biochip testing integrated machine's control system is the core hub ensuring stable operation and testing accuracy. It coordinates the various functional modules of the equipment and the actions of actuators such as temperature control, pipetting, and mechanical transfer. This system undertakes key tasks such as timing control of the testing process, contamination risk prevention, dynamic optimization of testing parameters, and handling of abnormal situations. It advances the testing process through data interaction and command transmission between modules, and its performance directly determines the equipment's testing accuracy, operational stability, and adaptability to different scenarios.

[0003] While existing intelligent biochip detection integrated machine control systems possess basic automation control capabilities, they suffer from significant shortcomings in terms of precision, fault tolerance, and high-throughput adaptability, making it difficult to meet current diverse testing needs. The core pain points are rigid anomaly response strategies and weak process fault tolerance: when faced with non-serious anomalies such as insufficient consumables or minor parameter fluctuations, a complete process shutdown is still employed, requiring restarting testing after troubleshooting. This prevents the use of historical data for continuation, significantly reducing the efficiency of high-throughput batch testing. To address these issues, this solution proposes an intelligent biochip detection integrated machine control system. Summary of the Invention

[0004] Technical problems to be solved To address the shortcomings of existing technologies, this invention provides an intelligent biochip detection integrated machine control system, which solves the problems mentioned in the background section.

[0005] Technical solution To achieve the above objectives, the present invention provides the following technical solution: a control system for an intelligent biochip detection integrated machine, comprising: The information matching module is used to collect information on the type and testing requirements of the chip to be tested, and to match the corresponding target workbench and testing process parameters. The transfer and dispensing module is used to perform the gripping, transfer and precise positioning of the chip between the chip chamber and the target worktable, as well as to perform the assembly of the dispensing needle, reagent aspiration, dispensing of the chip reaction wells and the removal and recycling of the dispensing needle after use. The reaction waste liquid module is used to output the temperature and mixing conditions required for the chip reaction according to the matched detection process parameters, and to perform the extraction and directional delivery of waste liquid in the chip reaction well. The imaging output module is used to perform focus calibration and aperture-by-aperture imaging of the chip reaction apertures, and to receive the acquired image data, perform analysis and processing, and output the chip detection results and a complete report.

[0006] Preferably, the specific steps for matching the corresponding target workbench with the detection process parameters are as follows: Receive the chip placement signal from the chip compartment, read the pre-entered basic chip information, extract the corresponding test items, reaction conditions and process requirements of the chip, and complete the integration of test requirement information; The system compares the target workbench with the preset workbench adaptation rules and simultaneously retrieves the temperature control parameters, hybridization parameters, reagent dispensing parameters, and reaction time parameters corresponding to the chip type.

[0007] Preferably, the specific steps for grasping, transferring, and precisely positioning the execution chip between the chip compartment and the target worktable are as follows: The system receives the target workbench information output by the information matching module, moves the gripper structure to directly above the chip to be tested, and performs a stable gripping action on the chip. The grasped chip is transported to the top of the target workbench, where it is precisely placed in the positioning slot of the workbench.

[0008] Preferably, the specific steps for performing the assembly of the sampling needle, reagent aspiration, filling of the chip reaction wells, and removal and recycling of the sampling needle after use are as follows: Upon receiving the trigger signal indicating that chip placement is complete, the pipetting structure moves to the needle storage area to complete the needle assembly. The pipetting structure is moved to the reagent storage area to complete the aspiration of the preset volume of reagent, and then moved to the top of the chip to complete the dispensing of reagent into the reaction well. The pipetting mechanism is moved to the recovery area to complete the removal and recovery of the used sample needle.

[0009] Preferably, the specific steps for outputting the temperature and mixing conditions required for the chip reaction based on the matched detection process parameters are as follows: Upon receiving the trigger signal indicating that reagent dispensing is complete, the hot cap is lowered and closed. Based on the matched detection process parameters, the corresponding workbench's heating, hybridization, or temperature rise / fall functions are activated. After reaching the preset reaction time, the hot cap is raised and reset, stopping the workbench's temperature control and hybridization actions.

[0010] Preferably, the specific steps for extracting and directionally transporting the waste liquid within the reaction hole of the execution chip are as follows: The system receives a trigger signal indicating the end of the chip reaction process, moves the liquid suction structure to directly above the chip to be processed, executes the descent and positioning action of the liquid suction needle, activates negative pressure to complete the extraction of waste liquid from a single hole, and controls the directional transport of the extracted waste liquid to the waste liquid collection chamber.

[0011] Preferably, the specific steps for focusing calibration and aperture-by-aperture imaging acquisition of the execution chip are as follows: Upon receiving the trigger signal indicating the end of waste liquid extraction, the imaging structure is moved to directly above the chip under test, and the focusing calibration action for chip imaging is performed. Turn on the supplementary light source and take high-definition photos of all reaction holes one by one according to the matrix arrangement of the chip holes; Turn off the supplementary light source and output the acquired image data.

[0012] Preferably, the specific steps for generating the output chip detection result and the complete report are as follows: The system receives chip image data, extracts grayscale values ​​and signal intensity data of each reaction hole, compares them with preset judgment thresholds to determine the results of each reaction hole, integrates the detection data by associating the chip's basic information and reaction process parameters, and finally outputs a complete chip detection report.

[0013] Preferably, the complete report output by the imaging output module includes basic chip information, reaction process parameters, detection results of each reaction well, original image, and statistical analysis data.

[0014] Beneficial effects The present invention has the following beneficial effects: (1) The present invention provides an integrated control system for an intelligent biochip detection machine. Through chip information recognition, process matching and unified coordination of the entire process execution mechanism, it realizes integrated adaptive control for the detection of multiple types of chips. No manual parameter setting is required. One system is compatible with the detection process of different chips, which greatly improves the versatility and automation.

[0015] (2) The present invention provides an intelligent biochip detection integrated machine control system, which realizes unmanned continuous control from chip loading to report output through signal triggering linkage of each functional link, solves the problem of fragmented links and poor connection, and improves detection efficiency and result stability.

[0016] Of course, any product implementing this invention does not necessarily need to achieve all of the advantages described above at the same time. Attached Figure Description

[0017] Figure 1 This is a structural diagram of the intelligent biochip detection integrated machine control system of the present invention. Detailed Implementation

[0018] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0019] This invention provides a technical solution: a control system for an intelligent biochip detection integrated machine, such as... Figure 1 As shown, it includes: The information matching module is used to collect information on the type and testing requirements of the chip to be tested, and to match the corresponding target workbench and testing process parameters. The specific steps for collecting information on the type of chip to be tested and testing requirements, and matching the corresponding target workbench and testing process parameters are as follows: The unique identification code of the chip to be tested is pre-entered through the human-machine interface of the equipment. After the chip placement sensor in the chip compartment detects that the chip has been placed, it sends a chip placement trigger signal to the control system. After receiving the trigger signal, the control system retrieves the pre-stored basic chip information through the chip's unique identification code, including chip model and specifications, number of targets, reaction well matrix arrangement, and detection item category. Extract the corresponding detection reaction conditions from the chip's basic information, including whether hybridization and mixing are required, the incubation temperature range, the reaction stage duration, and the reagent volume requirements, and integrate the detection requirement information. The hybridization method is to mix by oscillation. By comparing with the preset workbench adaptation rules, the detection items that require hybridization and constant temperature heating are matched to the first workbench, and the detection items that require high-precision temperature rise and fall closed-loop incubation are matched to the second workbench. Simultaneously, the temperature control parameters, hybridization frequency parameters, reagent dispensing parameters, and reaction time parameters corresponding to the chip type are retrieved to complete the full parameter matching of the detection process. The transfer and dispensing module is used to perform the gripping, transfer and precise positioning of the chip between the chip chamber and the target worktable, as well as to perform the assembly of the dispensing needle, reagent aspiration, dispensing of the chip reaction wells and the removal and recycling of the dispensing needle after use. The specific steps for gripping, transferring, and precisely positioning the execution chip between the chip chamber and the target worktable, assembling the sampling needle, aspirating reagents, filling the chip reaction wells, and removing and recycling the sampling needle after use are as follows: Before starting the entire equipment, initialize all parts and run them once to check the status. If the status is normal and correct, then proceed with subsequent use. If the status is abnormal (such as abnormal displacement of various components, inaccurate alignment, etc.), an alarm will be issued to prompt the staff to check and debug. Continue to operate after it is normal. The system receives the target workbench coordinate information output by the information matching module, and the control system feeds back the position data in real time through the servo encoder of the displacement mechanism. It calculates the optimal moving path of the gripper structure and drives the displacement mechanism to move the gripper structure along the preset trajectory to the top of the chip to be tested in the chip compartment. Once in position, the drive gripper structure descends vertically to the preset chip clamping height, and the two sets of clamping plates of the gripper open and close synchronously in opposite directions to complete the edge clamping and positioning of the chip. After clamping in position, the pressure sensor inside the clamping plate confirms the clamping status. After confirming stable clamping, the drive displacement mechanism moves the chip to the top of the positioning slot on the target worktable. The drive gripper structure descends vertically to the placement height, controls the two sets of clamping plates to open synchronously in opposite directions, and completes the precise placement of the chip in the positioning slot of the worktable. After the positioning sensor of the worktable confirms that the chip is in place, the gripper structure resets to the standby position. After the chip is placed in place, the positioning sensor of the workbench sends a trigger signal to the control system indicating that the chip placement is complete. The control system drives the displacement mechanism to move the pipetting structure to directly above the needle storage area in the reagent consumables compartment. The pipetting structure is then driven to descend vertically. The preset downward pressure causes the needle adapter of the pipetting structure to complete the interference fit assembly with the disposable needle. After the assembly is completed, the positioning sensor confirms the assembly status of the needle. After confirming that the assembly is complete, the drive pipetting structure moves to the top of the corresponding reagent tube in the reagent storage area and descends vertically to a preset depth below the reagent liquid surface when the tip of the pipetting needle is immersed. Through the precise stroke control of the pipetting pump, a volume of reagent matching the detection process parameters is drawn. After aspiration, the drive pipetting structure moves to directly above the corresponding reaction well on the chip, and controls the pipetting pump to complete the precise dispensing of reagents, thus realizing the reagent dispensing of the chip reaction well; After dispensing, the drive pipetting mechanism moves to directly above the waste consumables recycling area, and the push block mechanism on the pipetting mechanism moves downward to detach the used needle from the adapter and let it fall into the recycling channel to complete directional recycling. The pipetting mechanism then resets to the standby position. The reaction waste liquid module is used to output the temperature and mixing conditions required for the chip reaction according to the matched detection process parameters, and to perform the extraction and directional delivery of waste liquid in the chip reaction well. The specific steps for outputting the required temperature and mixing conditions for the chip reaction based on the matched detection process parameters, and for extracting and directionally transporting the waste liquid in the chip reaction wells are as follows: Upon receiving the trigger signal indicating that reagent dispensing is complete, the control system first drives the hot cap structure to descend vertically to the upper surface of the chip. After closing, it reaches the preset sealing pressure, thereby achieving full sealing and heat preservation of the chip's reaction holes. Then, based on the detection process parameters retrieved by the information matching module, the corresponding workbench function control is activated: If the target workbench is the first workbench, the PID closed-loop temperature control module is activated to heat the workbench to the preset target temperature. At the same time, the oscillation motor is activated to run at the preset hybridization frequency and amplitude to achieve the mixing of the chip reaction system. If the target workbench is the second workbench, the semiconductor cooling / heating module is activated, and the workbench temperature is controlled according to the preset temperature rise and fall curve to stabilize the temperature control accuracy within the preset range, thus meeting the requirements for precise chip incubation. After the preset reaction time is reached, the temperature control and hybridization actions of the worktable are stopped, and the hot cover structure is driven to rise and reset, completing the full-process reaction control of the chip. After the chip reaction process is completed, the control system sends a trigger signal to the waste liquid extraction unit, which drives the displacement mechanism to move the liquid suction structure to the top of the reaction hole of the chip to be processed, and drives the liquid suction needle to descend vertically to the preset depth in the chip reaction hole, so that the needle tip can fully contact the surface of the waste liquid without touching the bottom of the hole. Start the negative pressure pump and adjust the pressure of the negative pressure pipeline to the preset working pressure. Complete the extraction of waste liquid in a single hole according to the preset liquid suction time. The extracted waste liquid is directionally transported to the waste liquid collection chamber in the equipment base through a closed delivery pipeline. After the waste liquid extraction of all reaction holes of the chip is completed, drive the liquid suction structure to reset to the standby position. The imaging output module is used to perform focus calibration and aperture-by-aperture imaging of the chip reaction apertures, and to receive the acquired image data, perform analysis and processing, and output the chip detection results and a complete report. The specific steps for performing focus calibration and aperture-by-aperture imaging on the execution chip, and for analyzing and processing the acquired image data to output the chip detection results and a complete report are as follows: Upon receiving the trigger signal indicating the end of waste liquid extraction, the control system drives the displacement mechanism to move the imaging structure directly above the chip to be tested. The vertical lead screw drives the imaging structure to move up and down. Based on the real-time imaging clarity feedback of the chip aperture, automatic focusing calibration is completed. After focusing is completed, the vertical height of the imaging structure is locked. Turn on the supplementary light source that is coaxially arranged with the camera, adjust the brightness of the light source to the preset imaging supplementary light value, and drive the displacement mechanism to move the imaging structure row by row and column by column according to the matrix arrangement order of the chip reaction holes to complete the high-definition hole-by-hole imaging of all the reaction holes of the chip. After taking the picture, the supplementary light source is turned off, and the acquired full-hole image data is transmitted to the image processing unit of the control system. After receiving the chip image data, the image processing unit first performs preprocessing on the image, including grayscale correction, background noise reduction, and precise positioning of the reaction hole. Then, it extracts the average grayscale value, effective signal strength, and background signal difference of each reaction hole. The extracted signal data is compared with the preset positive / negative judgment thresholds to determine the detection result of each reaction well. Then, the basic information of the chip, the full-process operation parameters of the reaction process, and the original imaging images are linked to generate a standardized complete chip detection report. The report can be displayed in real time through the device's human-machine interface, exported locally via USB interface, or transmitted to the corresponding laboratory information management system via Ethernet.

[0020] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0021] The preferred embodiments of the present invention disclosed above are merely illustrative of the invention. These preferred embodiments do not exhaustively describe all details, nor do they limit the invention to the specific implementations described. Clearly, many modifications and variations can be made based on the content of this specification. This specification selects and specifically describes these embodiments to better explain the principles and practical applications of the invention, thereby enabling those skilled in the art to better understand and utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims

1. A control system for an intelligent biochip detection integrated machine, characterized in that, include: The information matching module is used to collect information on the type and testing requirements of the chip to be tested, and to match the corresponding target workbench and testing process parameters. The transfer and dispensing module is used to perform the gripping, transfer and precise positioning of the chip between the chip chamber and the target worktable, as well as to perform the assembly of the dispensing needle, reagent aspiration, dispensing of the chip reaction wells and the removal and recycling of the dispensing needle after use. The reaction waste liquid module is used to output the temperature and mixing conditions required for the chip reaction according to the matched detection process parameters, and to perform the extraction and directional delivery of waste liquid in the chip reaction well. The imaging output module is used to perform focus calibration and aperture-by-aperture imaging of the chip reaction apertures, and to receive the acquired image data, perform analysis and processing, and output the chip detection results and a complete report.

2. The intelligent biochip detection integrated machine control system according to claim 1, characterized in that, The specific steps for matching the corresponding target workbench with the detection process parameters are as follows: Receive the chip placement signal from the chip compartment, read the pre-entered basic chip information, extract the corresponding test items, reaction conditions and process requirements of the chip, and complete the integration of test requirement information; The system compares the target workbench with the preset workbench adaptation rules and simultaneously retrieves the temperature control parameters, hybridization parameters, reagent dispensing parameters, and reaction time parameters corresponding to the chip type.

3. The intelligent biochip detection integrated machine control system according to claim 1, characterized in that, The specific steps for grasping, transferring, and precisely positioning the execution chip between the chip compartment and the target worktable are as follows: The system receives the target workbench information output by the information matching module, moves the gripper structure to directly above the chip to be tested, and performs a stable gripping action on the chip. The grasped chip is transported to the top of the target workbench, where it is precisely placed in the positioning slot of the workbench.

4. The intelligent biochip detection integrated machine control system according to claim 1, characterized in that, The specific steps for assembling the sampling needle, aspirating reagents, filling the chip reaction wells, and removing and recycling the sampling needle after use are as follows: Upon receiving the trigger signal indicating that chip placement is complete, the pipetting structure moves to the needle storage area to complete the needle assembly. The pipetting structure is moved to the reagent storage area to complete the aspiration of the preset volume of reagent, and then moved to the top of the chip to complete the dispensing of reagent into the reaction well. The pipetting mechanism is moved to the recovery area to complete the removal and recovery of the used sample needle.

5. The intelligent biochip detection integrated machine control system according to claim 1, characterized in that, The specific steps for outputting the temperature and mixing conditions required for the chip reaction based on the matched detection process parameters are as follows: Upon receiving the trigger signal indicating that reagent dispensing is complete, the hot cap is lowered and closed. Based on the matched detection process parameters, the corresponding workbench's heating, hybridization, or temperature rise / fall functions are activated. After reaching the preset reaction time, the hot cap is raised and reset, stopping the workbench's temperature control and hybridization actions.

6. The intelligent biochip detection integrated machine control system according to claim 1, characterized in that, The specific steps for extracting and directionally transporting the waste liquid from the reaction hole of the execution chip are as follows: The system receives a trigger signal indicating the end of the chip reaction process, moves the liquid suction structure to directly above the chip to be processed, executes the descent and positioning action of the liquid suction needle, activates negative pressure to complete the extraction of waste liquid from a single hole, and controls the directional transport of the extracted waste liquid to the waste liquid collection chamber.

7. The intelligent biochip detection integrated machine control system according to claim 1, characterized in that, The specific steps for focusing calibration and aperture-by-aperture imaging acquisition using the execution chip are as follows: Upon receiving the trigger signal indicating the end of waste liquid extraction, the imaging structure is moved to directly above the chip under test, and the focusing calibration action for chip imaging is performed. Turn on the supplementary light source and take high-definition photos of all reaction holes one by one according to the matrix arrangement of the chip holes; Turn off the supplementary light source and output the acquired image data.

8. The intelligent biochip detection integrated machine control system according to claim 1, characterized in that, The specific steps for obtaining the output chip test results and complete report are as follows: The system receives chip image data, extracts grayscale values ​​and signal intensity data of each reaction hole, compares them with preset judgment thresholds to determine the results of each reaction hole, integrates the detection data by associating the chip's basic information and reaction process parameters, and finally outputs a complete chip detection report.

9. The intelligent biochip detection integrated machine control system according to claim 1, characterized in that, The complete report output by the imaging output module includes basic chip information, reaction process parameters, detection results of each reaction well, original image, and statistical analysis data.

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