Molecular marker of high-geraniol tea tree, marker primer, kit, application of molecular marker and identification method of high-geraniol tea tree
By developing molecular markers based on MITE insertion and deletion structural variations in the CsTPS15 gene promoter region, and using PCR amplification fragment length to distinguish high-geraniol tea germplasm, the problem of identification difficulties in existing technologies has been solved, enabling rapid and accurate germplasm identification and early screening, and improving the efficiency of tea breeding.
CN122235356APending Publication Date: 2026-06-19NORTHWEST A & F UNIV
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Patent Information
- Application Number
- CN202610555386.5
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2026-04-24
- Publication Date
- 2026-06-19
AI Technical Summary
Technical Problem
Existing technologies make it difficult to quickly and accurately identify high-geraniol tea germplasm, resulting in low tea breeding efficiency, and the detection methods are complex and costly.
Method used
Molecular markers based on MITE insertion and deletion structural variations in the promoter region of the CsTPS15 gene were developed, and specific PCR primers were designed. High-aroma terpene tea germplasm was distinguished by the difference in PCR amplification length, and different genotypes were distinguished by the length of PCR amplification fragments.
Benefits of technology
It enables rapid and accurate identification of high-fragrant tea germplasm, early screening of superior materials, improved breeding efficiency, and simplified testing process.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention belongs to the field of molecular biology, and particularly relates to a molecular marker of a high-geraniol tea tree, a marker primer, a kit as well as application and an identification method of the high-geraniol tea tree. Genomic sequences of tea tree materials with different geraniol contents are compared to find that a section of 261 bp MITE transposon insertion sequence exists in a CsTPS15 gene promoter region of the high-geraniol tea tree material; the sequence is deleted from the position of a common tea tree material, and the insertion of the MITE forms stable insertion deletion structure variation which is remarkably related to the geraniol content of the tea tree.
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